Robert Riehn
Nanofluidics, DNA, biopolymers, nanophotonics
Works (76)
Updated: July 1st, 2024 05:07
2024 article
Plasmonic interferometer for nanoscale refractometry
Ghaffari, A., & Riehn, R. (2024, June 10).
Source: ORCID
Added: July 1, 2024
2023 journal article
A nanophotonic interferometer
NANOTECHNOLOGY, 34(18).
author keywords: zero mode waveguide; sub-wavelength aperture; interferometer
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: January 19, 2023
2023 journal article
Assembly path dependence of telomeric DNA compaction by TRF1, TIN2, and SA1
BIOPHYSICAL JOURNAL, 122(10), 1822–1832.
TL;DR:
It is found that TRF1, TIN2, and SA1 work synergistically to cause a compaction of the DNA substrate, and that maximal compaction occurs if all three proteins are present.
(via Semantic Scholar)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: April 21, 2023
2022 article
A Nanophotonic Interferometer for small particle detection
INTERFEROMETRY XXI, Vol. 12223.
author keywords: zero mode waveguide; sub-wavelength aperture; interferometer
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: November 7, 2022
2022 journal article
Densely methylated DNA traps Methyl-CpG-binding domain protein 2 but permits free diffusion by Methyl-CpG-binding domain protein 3
JOURNAL OF BIOLOGICAL CHEMISTRY, 298(10).
MeSH headings : CpG Islands; DNA Methylation; DNA-Binding Proteins / metabolism; Mi-2 Nucleosome Remodeling and Deacetylase Complex / genetics; Mi-2 Nucleosome Remodeling and Deacetylase Complex / metabolism; Nucleosomes; Protein Binding; Transcription Factors / metabolism; Humans; Single Molecule Imaging
TL;DR:
These studies support a model in which MBD2-NuRD strongly localizes to and compacts methylated CpG islands while MBD3-NURD can freely mobilize nucleosomes independent of methylation status.
(via Semantic Scholar)
www.jbc.org (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: August 29, 2022
2022 article
Nanofluidic Device for Manipulation and Modification of DNA by Proteins
Dangi, S., Liu, M., Azad, Z., Countryman, P., Roushan, M., Livshits, G. I., … Riehn, R. (2022, December 30).
Source: ORCID
Added: July 1, 2024
2021 journal article
Structure, dynamics, and regulation of TRF1-TIN2-mediated trans- and cis-interactions on telomeric DNA
JOURNAL OF BIOLOGICAL CHEMISTRY, 297(3).
MeSH headings : Cell Adhesion Molecules / metabolism; Cell Adhesion Molecules / physiology; DNA / metabolism; DNA-Binding Proteins / metabolism; Humans; Microscopy, Atomic Force / methods; Models, Molecular; Multiprotein Complexes / metabolism; Protein Binding; Protein Isoforms / metabolism; Shelterin Complex / metabolism; Shelterin Complex / physiology; Telomere / metabolism; Telomere-Binding Proteins / metabolism; Telomere-Binding Proteins / physiology; Telomeric Repeat Binding Protein 1 / metabolism; Telomeric Repeat Binding Protein 1 / physiology; Telomeric Repeat Binding Protein 2 / metabolism; Telomeric Repeat Binding Protein 2 / physiology
TL;DR:
Analysis of DNA molecular structures promoted by TRF1-TIN2 interaction supports a molecular model in which protein assemblies at telomeres are heterogeneous with distinct subcomplexes and full shelterin complexes playing distinct roles in telomere protection and elongation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 26, 2021
2021 journal article
TIN2 is an architectural protein that facilitates TRF2-mediated trans- and cis-interactions on telomeric DNA
NUCLEIC ACIDS RESEARCH, 49(22), 13000–13018.
MeSH headings : Animals; DNA / chemistry; DNA / genetics; DNA / metabolism; HeLa Cells; Humans; Mice, Inbred C57BL; Microscopy, Atomic Force; Nucleic Acid Conformation; Protein Binding; Shelterin Complex / genetics; Shelterin Complex / metabolism; Telomere / genetics; Telomere / metabolism; Telomere-Binding Proteins / genetics; Telomere-Binding Proteins / metabolism; Telomeric Repeat Binding Protein 2 / genetics; Telomeric Repeat Binding Protein 2 / metabolism
TL;DR:
A molecular model in which TIN2 functions as an architectural protein to promote TRF2-mediated trans and cis higher-order nucleic acid structures at telomeres is proposed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 18, 2022
2020 journal article
Cohesin SA1 and SA2 are RNA binding proteins that localize to RNA containing regions on DNA
NUCLEIC ACIDS RESEARCH, 48(10), 5639–5655.
MeSH headings : Binding Sites; Cell Cycle Proteins / metabolism; Chromosomal Proteins, Non-Histone / metabolism; DNA / metabolism; R-Loop Structures; RNA / metabolism; RNA-Binding Proteins / metabolism
TL;DR:
It is discovered that both SA1 and SA2 bind to various RNA containing substrates, including ssRNA, dsRNA, RNA:DNA hybrids, and R-loops, and the majority of R-loop-localized SA1and SA2 are also sites where other subunits of the cohesin complex bind.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: October 5, 2020
2020 journal article
DNA looping by two 5-methylcytosine-binding proteins quantified using nanofluidic devices
EPIGENETICS & CHROMATIN, 13(1).
author keywords: DNA methylation; MeCP2; MBD2; DNA compaction
MeSH headings : 5-Methylcytosine / chemistry; 5-Methylcytosine / metabolism; Binding Sites; DNA / chemistry; DNA / metabolism; DNA Methylation; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / metabolism; Epigenomics / methods; Humans; Methyl-CpG-Binding Protein 2 / chemistry; Methyl-CpG-Binding Protein 2 / metabolism; Microfluidics / instrumentation; Microfluidics / methods; Microscopy, Atomic Force / methods; Protein Binding
TL;DR:
It is demonstrated that ATTO 565-labeled MBD2 is a good candidate as a staining agent for epigenetic mapping and determined that the mechanism for compaction by MeCP2 is the formation of bridges between distant DNA stretches and theformation of loops.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: April 20, 2020
2019 journal article
Cohesin SA2 and EWSR1 in R-Loop Regulation
Biophysical Journal, 116(3), 505a.
Sources: Crossref, NC State University Libraries
Added: January 25, 2020
2019 journal article
Direct observation of confinement-induced diffusophoresis
Nanotechnology, 7.
author keywords: nanofluidics; DNA; diffusophoresis
MeSH headings : DNA / chemistry; Diffusion; Ions / chemistry; Macromolecular Substances / chemistry; Microfluidics / methods; Nanotechnology / methods; Osmolar Concentration
TL;DR:
It is shown that long-range ionic strength gradients intrinsic to all nanofluidic devices, even at equilibrium, also drive a drift of macromolecules, which is the dominant cause of the drift diffusophoresis.
(via Semantic Scholar)
Source: ORCID
Added: July 23, 2019
2019 journal article
Nanoplumbing with 2D Metamaterials
SMALL, 15(2).
author keywords: confined polymers; DNA; nanofluidics
MeSH headings : DNA / chemistry; Hydrodynamics; Nanotechnology / methods
TL;DR:
A dynamic mean-field model of DNA at a nanochannel junction is proposed and it is shown that the dynamics of DNA in a nanofluidic device with branched channels and junctions is well described by the model.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: January 28, 2019
2019 journal article
Single-Molecule Study of TRF2 Mediated DNA Compaction using Physiologically Relevant Long Telomeric DNA
Biophysical Journal, 116(3), 505a.
Sources: Crossref, NC State University Libraries
Added: January 25, 2020
2019 journal article
TIN2 is an Architectural Protein Stabilizing TRF1 at Telomere
Biophysical Journal, 116(3), 211a–212a.
Sources: Crossref, NC State University Libraries
Added: January 25, 2020
2018 journal article
Cohesin SA2 is a sequence-independent DNA-binding protein that recognizes DNA replication and repair intermediates
JOURNAL OF BIOLOGICAL CHEMISTRY, 293(3), 1054–1069.
MeSH headings : Cell Cycle Proteins / chemistry; Cell Cycle Proteins / metabolism; Chromosomal Proteins, Non-Histone / chemistry; Chromosomal Proteins, Non-Histone / metabolism; DNA Repair / genetics; DNA Repair / physiology; DNA Replication / physiology; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / metabolism; Fluorescence Polarization; Genomic Instability / genetics; Genomic Instability / physiology; Microscopy, Atomic Force; Microscopy, Fluorescence; Protein Binding / genetics; Protein Binding / physiology
TL;DR:
The results suggest that SA2 functions at intermediate DNA structures during DNA transactions in genome maintenance pathways, and has important implications for understanding the function of cohesin in these pathways.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2018 journal article
DNA Methylation Detection Using Resonance and Nanobowtie-Antenna-Enhanced Raman Spectroscopy
BIOPHYSICAL JOURNAL, 114(11), 2498–2506.
MeSH headings : 5-Methylcytosine / metabolism; DNA Methylation; Nanotechnology; Spectrum Analysis, Raman
TL;DR:
It is shown that DNA carrying 5-methylcytosine modifications or methylated DNA (m-DNA) can be distinguished from DNA with unmodified cytosine by Raman spectroscopy enhanced by both a bowtie nanoantenna and excitation resonance, and the Raman resonance gain profile is consistent with A-term expectations.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2018 article
DNA methylation detection using UV nano bowtie antenna enhanced Raman spectroscopy
UV AND HIGHER ENERGY PHOTONICS: FROM MATERIALS TO APPLICATIONS 2018, Vol. 10727.
author keywords: Resonance Raman; methylation; cytosine; plasmonics; nano-antenna
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: December 3, 2018
2018 journal article
Manipulation and control of the electrokinetic motion of a non-conductive micro-particle in microchannel by generating lateral temperature gradient
International Journal of Heat and Mass Transfer, 126, 861–870.
author keywords: Charged particle; Electrophoresis; Temperature gradient; Lateral and rotational motion
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: December 10, 2020
2018 journal article
Motor-like DNA motion due to an ATP-hydrolyzing protein under nanoconfinement
Scientific Reports, 8(1).
MeSH headings : Adenosine Triphosphate / metabolism; DNA / metabolism; DNA Ligases / metabolism; DNA Ligases / physiology; DNA-Binding Proteins / genetics; Diffusion; Escherichia coli / genetics; Escherichia coli / metabolism; Escherichia coli Proteins / metabolism; Escherichia coli Proteins / physiology; Motion; MutS DNA Mismatch-Binding Protein / metabolism; MutS DNA Mismatch-Binding Protein / physiology; NAD / metabolism
TL;DR:
It is reported that long double-stranded DNA confined to quasi-1D nanochannels undergoes superdiffusive motion under the action of the enzyme T4 DNA ligase in the presence of necessary co-factors.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID, NC State University Libraries
Added: August 6, 2018
2017 journal article
CpG and methylation-dependent DNA binding and dynamics of the methylcytosine binding domain 2 protein at the single-molecule level
NUCLEIC ACIDS RESEARCH, 45(15), 9164–9177.
MeSH headings : 5-Methylcytosine / chemistry; 5-Methylcytosine / metabolism; Animals; Binding Sites; Chickens; Cloning, Molecular; CpG Islands; DNA / chemistry; DNA / metabolism; DNA Methylation; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Escherichia coli / genetics; Escherichia coli / metabolism; Fluorescence Polarization; Gene Expression; Humans; Magnetic Resonance Spectroscopy; Mi-2 Nucleosome Remodeling and Deacetylase Complex / metabolism; Mi-2 Nucleosome Remodeling and Deacetylase Complex / ultrastructure; Nucleic Acid Conformation; Nucleosomes / metabolism; Nucleosomes / ultrastructure; Protein Binding; Protein Isoforms / chemistry; Protein Isoforms / genetics; Protein Isoforms / metabolism; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Single Molecule Imaging
TL;DR:
The results suggest that MBD2 targets NuRD to unmethylated or methylated CpG islands where its distinct dynamic binding modes help maintain open or closed chromatin, respectively.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
14. Life Below Water
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2016 journal article
Dynamics of Large DNA Loops
Biophysical Journal, 110(3), 565a.
TL;DR:
This work uses a nanofluidic device to manipulate fluid flow to drive DNA into large loops, on the order of kilobasepairs, in a range of buffer concentrations and protein backgrounds, and quantifies an energy landscape to characterize activation barriers for proteins to form DNA loops.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: January 25, 2020
2016 journal article
Enhanced electrostatic force microscopy reveals higher-order DNA looping mediated by the telomeric protein TRF2
Scientific Reports, 6(1).
MeSH headings : DNA / chemistry; DNA / metabolism; HeLa Cells; Humans; Microscopy; Multiprotein Complexes / chemistry; Multiprotein Complexes / metabolism; Nucleic Acid Conformation; Static Electricity; Telomeric Repeat Binding Protein 2 / chemistry; Telomeric Repeat Binding Protein 2 / metabolism
TL;DR:
Supporting coarse-grained molecular dynamics simulations uncover the structural requirement and sequential steps during TRF2-mediated DNA compaction and result in folded DNA structures with protruding DNA loops as seen in DREEM imaging.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, NC State University Libraries, NC State University Libraries
Added: August 6, 2018
2016 journal article
Functional interplay between SA1 and TRF1 in telomeric DNA binding and DNA-DNA pairing
NUCLEIC ACIDS RESEARCH, 44(13), 6363–6376.
MeSH headings : AT-Hook Motifs / genetics; Chromatids / genetics; Chromatids / ultrastructure; Chromosome Segregation / genetics; DNA-Binding Proteins / genetics; Humans; Microscopy, Atomic Force; Mitosis / genetics; Nuclear Proteins / genetics; Nuclear Proteins / metabolism; Telomere / genetics; Telomere / ultrastructure; Telomere-Binding Proteins / genetics; Telomere-Binding Proteins / metabolism; Telomeric Repeat Binding Protein 1 / genetics; Telomeric Repeat Binding Protein 1 / metabolism
TL;DR:
The results suggest that at telomeres cohesion relies on the molecular interplay between TRF1 and SA1 to promote DNA–DNA pairing, while along chromosomal arms the core cohesin assembly might also depend on SA1 1D diffusion on DNA and sequence-specific DNA binding.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2016 journal article
Nonaffine deformation under compression and decompression of a flow-stabilized solid
JOURNAL OF STATISTICAL MECHANICS-THEORY AND EXPERIMENT, 2016(8).
author keywords: Brownian motion; colloidal glasses; elasticity; plasticity
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2015 journal article
Collapse of DNA under alternating electric fields
PHYSICAL REVIEW E, 92(1).
MeSH headings : DNA / chemistry; DNA Contamination; Electromagnetic Phenomena; Ions / chemistry; Microscopy, Fluorescence; Video Recording
TL;DR:
An in-depth study of the collapse of DNA under weak confinement in microchannels as a function of buffer strength, driving frequency, applied electric-field strength, and molecule size is provided.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2015 journal article
DNA Brushing Shoulders: Targeted Looping and Scanning of Large DNA Strands
Nano Letters, 15(8), 5641–5646.
Contributors: Z. Azad n, M. Roushan n & n
author keywords: Nanofluidics; DNA; DNA loops; fluorescence microscopy; nanoconfinement; single-molecule
MeSH headings : DNA / chemistry; Equipment Design; Lab-On-A-Chip Devices; Microscopy, Fluorescence; Nanotechnology / instrumentation; Nucleic Acid Conformation
TL;DR:
The folding and unfolding of hairpin-shaped regions, similar to chromatin loops, are demonstrated and a model governing the stability of loop folds is presented and how the system achieves specific DNA configurations without operator intervention is discussed.
(via Semantic Scholar)
Sources: ORCID, NC State University Libraries, NC State University Libraries
Added: August 6, 2018
2015 journal article
Interference of ATP with the fluorescent probes YOYO-1 and YOYO-3 modifies the mechanical properties of intercalator-stained DNA confined in nanochannels
Microchimica Acta, 182(7-8), 1561–1565.
author keywords: DNA; ATP; Intercalating dye; YOYO-1; DAPI; Nanofluidics
TL;DR:
Investigation of the mechanical properties of DNA stained with the fluorescent intercalating dyes YOYO-1 and YoyO-3 as a function of ATP concentrations by stretching single molecules in nanofluidic channels finds negligible effects if DNA is visualized with the minor groove-binding probe 4′,6-diamidino-2-phenylindole.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018
2014 journal article
Nonlinear elasticity of microsphere heaps
Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics, 90(2), 9.
Contributors: C. Ortiz n, K. Daniels n & n
MeSH headings : Elasticity; Microfluidic Analytical Techniques; Microscopy, Fluorescence; Microspheres; Models, Theoretical; Nonlinear Dynamics
TL;DR:
This analysis indicates that many-body interactions contribute a significant fraction of the stress supported by the heap, and the measured stress-strain curves exhibit a universal nonlinear shape, which can be predicted from a thermal van der Waals equation of state with excluded volume.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
10. Reduced Inequalities
(OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2014 journal article
Probing transient protein-mediated DNA linkages using nanoconfinement
Biomicrofluidics, 8(3), 034113.
Contributors: M. Roushan n, P. Kaur n, A. Karpusenko n, P. Countryman n, C. Ortiz n, S. Lim n , H. Wang n , n
TL;DR:
The study of the interaction between T4 DNA ligase and DNA concludes that ligase can transiently stabilize folded DNA configurations by coordinating genetically distant DNA stretches.
(via Semantic Scholar)
Sources: ORCID, NC State University Libraries, Crossref, NC State University Libraries
Added: August 6, 2018
2014 conference paper
Single-molecule imaging reveals DNA-binding properties of cohesin proteins SA1 and SA2.
Environmental and Molecular Mutagenesis, 55, S29–29.
Source: NC State University Libraries
Added: August 6, 2018
2013 journal article
Chromatin modification mapping in nanochannels
Biomicrofluidics, 7(6), 064105.
Contributors: S. Lim n , A. Karpusenko n, A. Blumers n, D. Streng n & n
TL;DR:
It is determined that the major difficulty in transitioning the mapping method to site-specific profiling within single genomic molecules is the interference of naturally aggregating, off-the shelf antibodies with the internal structure of chromatin.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water
(OpenAlex)
Sources: ORCID, NC State University Libraries, Crossref, NC State University Libraries
Added: August 6, 2018
2013 journal article
DNA Looping Induced by Tubular Confinement
Biophysical Journal, 104(2), 253a–254a.
TL;DR:
The fact that the formation of hairpins and the 1-d scanning of strands past each other solves the kinetic problem of forming a loop in a maze-like environment is pointed to.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 8, 2020
2013 journal article
Flow-driven formation of solid-like microsphere heaps
SOFT MATTER, 9(2), 543–549.
Contributors: C. Ortiz n
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2013 journal article
SENSING DNA WITH ALTERNATING CURRENTS USING A NANOGAP SENSOR EMBEDDED IN A NANOCHANNEL DEVICE
Nano LIFE, 03(01), 1340007.
Contributors: J. H. Davis n
author keywords: DNA sequencing; nanochannels; nanoelectrodes
TL;DR:
An integrated nanochannel/nanoelectrode sensor for the detection of DNA using alternating currents is reported, and it is found that DNA can be detected using platinum as the metal for the detecting electrodes, with a signal to noise ratio exceeding 10.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Source: ORCID
Added: July 23, 2019
2012 journal article
Fluctuation modes of nanoconfined DNA
Journal of Applied Physics, 111(2), 024701.
TL;DR:
An experimental investigation of the magnitude of length and density fluctuations in DNA that has been stretched in nanofluidic channels finds that the experimental data can be described using a one-dimensional overdamped oscillator chain with nonzero equilibrium spring length and that a chain of discrete oscillators yields a better description than a continuous chain.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries, NC State University Libraries
Added: August 6, 2018
2012 journal article
Near-field enhanced ultraviolet resonance Raman spectroscopy using aluminum bow-tie nano-antenna
Applied Physics Letters, 101(11).
Contributors: L. Li n
TL;DR:
An aluminum bow-tie nano-antenna is combined with the resonance Raman effect in the deep ultraviolet to dramatically increase the sensitivity of Raman spectra to a small volume of material, such as benzene used here.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: July 23, 2019
2012 journal article
Near-field enhanced ultraviolet resonance Raman spectroscopy using aluminum bow-tie nano-antenna
Applied Physics Letters, 101(11).
Source: NC State University Libraries
Added: August 6, 2018
2011 journal article
MeSH headings : DNA / chemistry; Electricity; Microfluidics; Models, Molecular
TL;DR:
It is reported that double-stranded DNA collapses in the presence of ac electric fields at frequencies of a few hundred Hertz, and does not stretch as commonly assumed.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2011 journal article
DNA methylation profiling in nanochannels
Biomicrofluidics, 5(3).
Contributors: S. Fang Lim n
author keywords: biochemistry; DNA; genetics; molecular biophysics; proteins
TL;DR:
The profiling of the 5-methyl cytosine distribution within single genomic-sized DNA molecules at a gene-relevant resolution is reported, and single-molecule read-lengths exceeding 140 kbp have been achieved.
(via Semantic Scholar)
Sources: ORCID, NC State University Libraries, NC State University Libraries
Added: August 6, 2018
2011 journal article
Density fluctuations dispersion relationship for a polymer confined to a nanotube
Applied Physics Letters, 98(25), 253704.
Contributors: J. Carpenter n
TL;DR:
It is found that for highly elongated molecules a Rouse description is consistent with observations at sufficiently large length scales and that for strongly fluctuating molecules, or short length scales, such Rouse modes cannot be detected due to strong mixing of fluctuation modes.
(via Semantic Scholar)
Sources: ORCID, NC State University Libraries, Crossref, NC State University Libraries
Added: August 6, 2018
2010 journal article
Epigenetic Analysis of Chromatin in Nanochannels
Biophysical Journal, 98(3), 600a.
TL;DR:
It is shown here that the concept by nanoconfinement can be extended to chromatin, or DNA complexed to histones, and that the stretching is within the range expected from the de Gennes theory.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: September 8, 2020
2009 journal article
Complementary metal oxide semiconductor compatible fabrication and characterization of parylene-C covered nanofluidic channels with integrated nanoelectrodes
BIOMICROFLUIDICS, 3(3).
Contributors: C. Tung*
TL;DR:
A near-room-temperature fabrication technology involving parylene-C conformal deposition that is compatible with complementary metal oxide semiconductor electronic devices is presented and an analysis of the initial impedance measurements of conformally paryene-C coated nanochannels with integrated gold nanoelectrodes is presented.
(via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2009 journal article
DNA Condensation by Field-Induced Non-Equilibrium Noise
CHEMPHYSCHEM, 10(16), 2871–2875.
Contributors: R. Bruinsma *
author keywords: DNA; electric field; equilibrium; gel electrophoresis; noise
MeSH headings : Algorithms; DNA / chemistry; Solvents / chemistry; Static Electricity; Temperature
TL;DR:
It is shown that AC electrical fields couple charge fluctuations to molecular shape fluctuations, which produces a non-equilibrium noise source that can dominate over equilibrium thermal noise in the long-wavelength limit.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2009 journal article
Stretching chromatin through confinement
LAB ON A CHIP, 9(19), 2772–2774.
Contributors: D. Streng n
MeSH headings : Chromatin / chemistry; Chromatin / genetics; DNA / chemistry; DNA / genetics; Microfluidic Analytical Techniques / methods
TL;DR:
The stretching of chromatin to about 12 basepairs/nm enables location-resolved optical investigation of the nucleic material with a resolution of up to 6 kbp and finds that chromatin is 2.5 times more compact in its stretched state.
(via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2009 journal article
MeSH headings : Diagnostic Imaging / methods; Nanoparticles / chemistry; Nanotechnology / methods
TL;DR:
Sub-10 nm, upconverting rare earth oxide UCNPs synthesized by a combustion method that can be stably suspended in water when amine modified are demonstrated and show specific surface immobilization onto patterned gold surfaces.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
Sources: ORCID, NC State University Libraries, Crossref, NC State University Libraries
Added: August 6, 2018
2008 journal article
Single molecule correlation spectroscopy in continuous flow mixers with zero-mode waveguides
Optics Express, 16(14), 10077–10090.
Contributors: D. Liao
MeSH headings : Equipment Design; Fluorescent Dyes / pharmacology; Kinetics; Light; Models, Statistical; Models, Theoretical; Nanotechnology / methods; Optics and Photonics; Photons; Spectrometry, Fluorescence / instrumentation; Spectrometry, Fluorescence / methods; Time Factors
TL;DR:
It is shown that combining zero-mode waveguides with fluorescence correlation spectroscopy in a continuous flow mixer avoids the compression of the FCS signal due to fluid transport at channel velocities up to approximately 17 mm/s, making FCS suitable for direct kinetics measurements in rapid continuous flow.
(via Semantic Scholar)
Source: ORCID
Added: July 23, 2019
2008 review
The potential and challenges of nanopore sequencing
[Review of ]. NATURE BIOTECHNOLOGY, 26(10), 1146–1153.
Contributors: D. Branton*
MeSH headings : Chromosome Mapping / trends; DNA / chemistry; DNA / genetics; Forecasting; Genomics / trends; Nanostructures / chemistry; Nanostructures / ultrastructure; Nanotechnology / trends; Sequence Alignment / trends; Sequence Analysis, DNA / trends
TL;DR:
A nanopore-based device provides single-molecule detection and analytical capabilities that are achieved by electrophoretically driving molecules in solution through a nano-scale pore, a unique analytical capability that makes inexpensive, rapid DNA sequencing a possibility.
(via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2006 journal article
MeSH headings : DNA / chemistry; DNA / radiation effects; Electromagnetic Fields; Electrophoresis / methods; Microfluidics / instrumentation; Microfluidics / methods; Motion; Nanostructures / chemistry; Nucleic Acid Conformation / radiation effects; Silicon Dioxide / chemistry; Surface Properties
TL;DR:
A metamaterial consisting of a two-dimensional, asymmetric lattice of crossed nanochannels in fused silica, with channel diameters of 80 nm to 140 nm, shows that the asymmetry in channel dimensions gives rise to a preferred direction for DNA orientation and a preferreddirection for transport under dc electrophoresis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Source: ORCID
Added: July 23, 2019
2006 journal article
Diffusion mechanisms of localised knots along a polymer
Europhysics Letters, 76(4), 696–702.
Contributors: R. Metzler *
UN Sustainable Development Goal Categories
11. Sustainable Cities and Communities
(OpenAlex)
Source: ORCID
Added: July 23, 2019
2006 journal article
In Vivo and Scanning Electron Microscopy Imaging of Upconverting Nanophosphors inCaenorhabditiselegans
Nano Letters, 6(2), 169–174.
Contributors: S. Lim*
MeSH headings : Animals; Caenorhabditis elegans / chemistry; Microscopy, Electron, Scanning / methods; Nanostructures / chemistry; Particle Size; Phosphorus / chemistry; Sensitivity and Specificity; Spectrometry, X-Ray Emission / methods; Surface Properties
TL;DR:
It is shown here that upconversion phosphors can be imaged both by infrared excitation and in a scanning electron microscope, and it is confirmed using energy-dispersive X-ray analysis that the up-conversion nanoparticles can be identified in a scan electron microscope at high spatial resolution.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Sources: ORCID, Crossref, NC State University Libraries
Added: July 23, 2019
2006 journal article
Local probing of photocurrent and photoluminescence in a phase-separated conjugated-polymer blend by means of near-field excitation
Advanced Functional Materials, 16(4), 469–476.
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Source: ORCID
Added: July 23, 2019
2006 journal article
Microfluidic high gradient magnetic cell separation
Journal of Applied Physics, 99(8).
Contributors: D. Inglis *
TL;DR:
The basic principles of how forces are generated via the magnetic susceptibility of an object are discussed and how microfluidics can be combined with micron-scale magnetic field gradients to greatly enhance in principle the fractionating power of magnetic fields are discussed.
(via Semantic Scholar)
Source: ORCID
Added: July 23, 2019
2006 journal article
Use of sub-10 nm diameter upconversion nanophosphors as bio-labels
Materials Research Society Symposium Proceedings, 950, 174–179. http://www.scopus.com/inward/record.url?eid=2-s2.0-40949090219&partnerID=MN8TOARS
Contributors: S. Lim
Source: ORCID
Added: July 23, 2019
2006 journal article
Wetting micro- and nanofluidic devices using supercritical water
ANALYTICAL CHEMISTRY, 78(16), 5933–5934.
MeSH headings : Microfluidic Analytical Techniques; Nanotechnology; Temperature; Water
TL;DR:
Wetting of micro- and nanostructures in a fused-silica device with only a single inlet is demonstrated, which is low-cost, fast, safe, and very reliable.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2005 journal article
A two-dimensional photonic structure made from a conjugated, fluorescent polymer
Journal of Optics A: Pure and Applied Optics, 7(2).
author keywords: conjugated polymers; SNOM; near-field lithography; PPV; organic semiconductors; photonic structures
Source: ORCID
Added: July 23, 2019
2005 journal article
DNA statics and dynamics in nanoscale confinement
Proceedings of SPIE - The International Society for Optical Engineering, 5592, 193–200.
Contributors: W. Reisner *
author keywords: nanochannel; DNA
Source: ORCID
Added: July 23, 2019
2005 journal article
Restriction mapping in nanofluidic devices
Proceedings of the National Academy of Sciences, 102(29), 10012–10016.
MeSH headings : DNA / metabolism; DNA Restriction Enzymes / metabolism; Magnesium; Microscopy; Nanostructures; Nanotechnology / methods; Restriction Mapping / methods
TL;DR:
This work has performed restriction mapping of DNA molecules using restriction endonucleases in nanochannels with diameters of 100-200 nm and has been able to measure the positions of restriction sites with a precision of approximately 1.5 kbp in 1 min using single DNA molecules.
(via Semantic Scholar)
Sources: ORCID, Crossref, NC State University Libraries
Added: July 23, 2019
2005 journal article
Single-molecule studies of repressor-DNA interactions show long-range interactions
Proceedings of the National Academy of Sciences of the United States of America, 102(28), 9796–9801.
Contributors: Y. Wang*
author keywords: binding coefficient; multiple sites; transcription factors
MeSH headings : Bacteriophage lambda / genetics; Biophysical Phenomena; Biophysics; Carbocyanines; DNA, Viral / metabolism; DNA-Binding Proteins / isolation & purification; Electrophoresis / methods; Green Fluorescent Proteins / metabolism; Nanotechnology / methods; Operator Regions, Genetic / genetics; Repressor Proteins / metabolism
TL;DR:
This single-molecule studies of GFP-LacI repressor proteins bound to bacteriophage lambda DNA containing a 256 tandem lac operator insertion confined in nanochannels suggest that the repressors influence each other even when they are widely separated, at distances on the order of 200 nm, or several DNA persistence lengths.
(via Semantic Scholar)
Source: ORCID
Added: July 23, 2019
2005 journal article
Statics and dynamics of single DNA molecules confined in nanochannels
Physical Review Letters, 94(19).
Contributors: W. Reisner *
MeSH headings : Bacteriophage lambda / chemistry; Bacteriophage lambda / genetics; DNA, Viral / chemistry; DNA, Viral / immunology; Nanotechnology / methods; Nanotubes / chemistry; Silicon Dioxide / chemistry
TL;DR:
Measurements of DNA extended in nanochannels are presented and it is shown that below a critical width roughly twice the persistence length there is a crossover in the polymer physics.
(via Semantic Scholar)
Source: ORCID
Added: July 23, 2019
2004 journal article
Continuous microfluidic immunomagnetic cell separation
Applied Physics Letters, 85(21), 5093–5095.
Contributors: D. Inglis *
TL;DR:
A continuous-flow microfluidic device is presented that enables cell by cell separation of cells selectively tagged with magnetic nanoparticles and has been demonstrated by the separation of leukocytes from whole human blood.
(via Semantic Scholar)
Source: ORCID
Added: July 23, 2019
2004 journal article
Fabrication of conjugated polymers nanostructures via direct near-field optical lithography
Ultramicroscopy, 100(3-4), 449–455.
Contributors: F. Cacialli *
author keywords: conjugated polymers; nanostructures; direct near-field optical lithography
TL;DR:
The use of the technique for the direct writing of two-dimensional periodic structures with intentional defects and a periodicity relevant to applications in the visible range is demonstrated.
(via Semantic Scholar)
Source: ORCID
Added: July 23, 2019
2004 journal article
The dynamics of genomic-length DNA molecules in 100-nm channels
Proceedings of the National Academy of Sciences of the United States of America, 101(30), 10979–10983.
Contributors: J. Tegenfeldt *
MeSH headings : DNA / chemistry; DNA / genetics; Genome; Kinetics; Models, Molecular; Nucleic Acid Conformation; Sensitivity and Specificity
Source: ORCID
Added: July 23, 2019
2003 journal article
Near-field optical lithography of a conjugated polymer
Applied Physics Letters, 82(4), 526–528.
Source: ORCID
Added: July 23, 2019
2002 journal article
Near-field photoconductivity imaging of a conjugated polymer blend
Materials Research Society Symposium - Proceedings, 708, 71–76. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036054160&partnerID=MN8TOARS
Source: ORCID
Added: July 23, 2019
2001 journal article
Efficient electroluminescent poly(p-phenylene vinylene) copolymers for application in LEDs
Synthetic Metals, 119(1-3), 43–44.
Contributors: R. Martin
author keywords: electroluminescence; photoluminescence; conjugated polymers; poly(p-phenylene vinylene); light-emitting devices
Source: ORCID
Added: July 23, 2019
2001 journal article
Erratum: Ultraviolet-visible near-field microscopy of phase-separated blends of polyfluorene-based conjugated semiconductors (Applied Physics Letters (2001) 79:833)
Applied Physics Letters, 79(12).
Contributors: R. Stevenson *
Source: ORCID
Added: July 23, 2019
2001 journal article
Fluorine-substituted poly(p-phenylenes vinylenes) copolymers
Synthetic Metals, 124(1), 67–69.
author keywords: PPV; fluorine; light emitting diodes; LED
Source: ORCID
Added: July 23, 2019
2001 journal article
New routes to monomers and polymers for LEDs
Proceedings of SPIE - The International Society for Optical Engineering, 4105, 53–58.
Contributors: A. Holmes *
Source: ORCID
Added: July 23, 2019
2001 journal article
Synthesis of new building blocks for light emitting polymers
Materials Research Society Symposium - Proceedings, 660. http://www.scopus.com/inward/record.url?eid=2-s2.0-0035559523&partnerID=MN8TOARS
Contributors: C. Fischmeister
Source: ORCID
Added: July 23, 2019
2001 journal article
Ultraviolet-visible near-field microscopy of phase-separated blends of polyfluorene-based conjugated semiconductors
Applied Physics Letters, 79(6), 833–835.
Contributors: R. Stevenson *
Source: ORCID
Added: July 23, 2019
2001 journal article
Versatile synthesis of various conjugated aromatic homo- and copolymers
Synthetic Metals, 122(1), 1–5.
Contributors: R. Martin *
author keywords: gilch polymerisation; conjugated polymers; light emitting diodes
Source: ORCID
Added: July 23, 2019
2000 journal article
Application and improvement of the spreading resistance method for p-type 6H-SiC
Materials Science Forum, 338. http://www.scopus.com/inward/record.url?eid=2-s2.0-0033723197&partnerID=MN8TOARS
Contributors: T. Gebel
Source: ORCID
Added: July 23, 2019
2000 journal article
Efficient blue-green light emitting poly(1,4-phenylene vinylene) copolymers
Chemical Communications, (4), 291–292. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034696226&partnerID=MN8TOARS
Contributors: R. Martin
Source: ORCID
Added: July 23, 2019
2000 journal article
Electrochemical and electroluminescent properties of random copolymers of fluorine- and alkoxy-substituted poly(p-phenylene vinylene)s
Macromolecules, 33(9), 3337–3341.
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: July 23, 2019
2000 journal article
Versatile syntheses of various homo- and copolymers of poly(1,4-arylene vinylene)s
Materials Research Society Symposium - Proceedings, 598. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034499504&partnerID=MN8TOARS
Contributors: R. Martin
Source: ORCID
Added: July 23, 2019
Employment
Updated: January 5th, 2023 13:46
2006 - present
North Carolina State University Raleigh, NC, US
Asst. Prof/ Assoc. Prof/ Prof
Physics
2003 - 2006
Princeton University Princeton, NJ, US
Postdoc
Physics
Education
Updated: December 9th, 2020 17:54
1999 - 2003
University of Cambridge Cambridge, Cambridgeshire, GB
Ph.D.
Physics
1998 - 1998
University of Cape Town Rondebosch, Western Cape, ZA
B.Sc. (hons)
Physics
1994 - 1997
Technische Universität Clausthal Clausthal-Zellerfeld, Niedersachsen, DE
Vordiplom
Physics
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