Works (7)

Updated: July 31st, 2024 05:04

2024 article

Characterization of <i>Ligilactobacillus salivarius</i> CRISPR-Cas systems

Roberts, A., Spang, D., Sanozky-Dawes, R., Nethery, M. A., & Barrangou, R. (2024, July 11). (C. D. Ellermeier, Ed.). MSPHERE, Vol. 7.

By: A. Roberts n, D. Spang n, R. Sanozky-Dawes n, M. Nethery n & R. Barrangou n

Ed(s): C. Ellermeier

author keywords: Ligilactobacillus; salivarius; CRISPR; Cas
Sources: Web Of Science, ORCID, NC State University Libraries
Added: July 15, 2024

2020 journal article

Comparative Analysis of Lactobacillus gasseri and Lactobacillus crispatus Isolated From Human Urogenital and Gastrointestinal Tracts

FRONTIERS IN MICROBIOLOGY, 10.

By: M. Pan n, C. Hidalgo-Cantabrana n, Y. Goh n, R. Sanozky-Dawes n & R. Barrangou n

author keywords: probiotics; niche-specific adaptation; Lactobacillus; women's health; in vitro vaginal fluid model
Sources: Web Of Science, NC State University Libraries
Added: February 17, 2020

2018 journal article

Deletion-based escape of CRISPR-Cas9 targeting in Lactobacillus gasseri

MICROBIOLOGY-SGM, 164(9), 1098–1111.

By: E. Stout n, R. Sanozky-Dawes n, Y. Goh n, A. Crawley n, T. Klaenhammer n & R. Barrangou n

author keywords: Lactobacillus; CRISPR; Cas; Cas9; interference; spacer
MeSH headings : CRISPR-Associated Protein 9 / metabolism; Clustered Regularly Interspaced Short Palindromic Repeats; Interspersed Repetitive Sequences; Lactobacillus gasseri / genetics; Mutation; Plasmids; Recombination, Genetic; Sequence Deletion; Transformation, Bacterial
TL;DR: It is shown that deletions of spacers within CRISPR arrays constitute a key escape mechanism to evadeCRISPR targeting, while preserving the functionality of the CRISpr-Cas system. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: December 31, 2018

2018 review

Insights into the Human Virome Using CRISPR Spacers from Microbiomes

[Review of ]. VIRUSES-BASEL, 10(9).

By: C. Hidalgo-Cantabrana n, R. Sanozky-Dawes n & R. Barrangou n

author keywords: CRISPR-Cas systems; CRISPR spacers; virome; phages; microbiome
MeSH headings : Biodiversity; Clustered Regularly Interspaced Short Palindromic Repeats; Humans; Metagenomics; Microbiota; Viruses / classification; Viruses / genetics; Viruses / isolation & purification
TL;DR: It is shown how the CRISPR spacer content of commensal and pathogenic bacteria can be used to determine the evidence of their phage exposure, which opens new opportunities for investigating host-virus dynamics in metagenomic data, and highlights the need for more efforts for virome sampling and sequencing. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: November 26, 2018

2018 journal article

Phenotypic and genotypic diversity of Lactobacillus buchneri strains isolated from spoiled, fermented cucumber

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 280, 46–56.

By: K. Daughtry n, S. Johanningsmeier n, R. Sanozky-Dawes n, T. Klaenhammer n & R. Barrangou n

author keywords: Lactic acid bacteria; Pickled vegetables; Spoilage organisms; Lactobacillus buchneri; Strain diversity
MeSH headings : Acetic Acid / metabolism; Bioreactors; Cucumis sativus / microbiology; Fermentation; Genotype; Lactic Acid / metabolism; Lactobacillus / classification; Lactobacillus / genetics; Lactobacillus / isolation & purification; Lactobacillus / metabolism; Propylene Glycol / metabolism; RNA, Ribosomal, 16S / genetics
TL;DR: Describing isolates of L. buchneri purified from both industrial and experimental fermented cucumber after the onset of secondary fermentation provided the basis for further analysis of metabolic and genomic capabilities of this species to enable control of lactic acid degradation in fermented plant materials. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2015 journal article

Conserved S-Layer-Associated Proteins Revealed by Exoproteomic Survey of S-Layer-Forming Lactobacilli

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(1), 134–145.

By: B. Johnson n, J. Hymes n, R. Sanozky-Dawes n, E. Henriksen n, R. Barrangou n & T. Klaenhammer n

Ed(s): H. Nojiri

MeSH headings : Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Lactobacillus / chemistry; Lactobacillus / classification; Lactobacillus / genetics; Lactobacillus / metabolism; Lactobacillus acidophilus / chemistry; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / metabolism; Membrane Glycoproteins / chemistry; Membrane Glycoproteins / genetics; Membrane Glycoproteins / metabolism; Molecular Sequence Data; Phylogeny; Proteomics
TL;DR: Proteomic complexity and differences in the cell surface properties between S-layer- and non-S- layer-forming lactobacilli reveal the potential for SLAPs to mediate intimate probiotic interactions and signaling with the host intestinal mucosa. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2015 journal article

Occurrence and activity of a type II CRISPR-Cas system in Lactobacillus gasseri

MICROBIOLOGY-SGM, 161, 1752–1761.

By: R. Sanozky-Dawes n, K. Selle n, S. O'Flaherty n, T. Klaenhammer n & R. Barrangou n

MeSH headings : Base Sequence; CRISPR-Cas Systems / genetics; Clustered Regularly Interspaced Short Palindromic Repeats; Gene Order; Genome, Bacterial; Lactobacillus / genetics; Molecular Sequence Data; Plasmids / genetics
TL;DR: The distribution and function of a native type II-A CRISPR-Cas system in the commensal species L. gasseri is reported, which open avenues for applications for bacteriophage protection and genome modification in L.gasseri, and contribute to the fundamental understanding of CRISpr-Cas systems in bacteria. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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