@article{roode_shive_hoorntje_bernard_stowe_pool_grindem_2018, title={Multiloculated solitary (unicameral) bone cyst in a young dog}, volume={47}, ISSN={0275-6382}, url={http://dx.doi.org/10.1111/vcp.12618}, DOI={10.1111/vcp.12618}, abstractNote={AbstractA 20‐month‐old female spayed Staffordshire Terrier (22.3 kg) presented to the Orthopedic Surgery Service at North Carolina State University Veterinary Teaching Hospital for evaluation of a 6‐week history of toe‐touching to nonweight‐bearing lameness in the right hind limb. Radiographs of the right stifle revealed a multiloculated lytic lesion of the distal femur, with a large open lytic zone centrally, numerous osseous septations peripherally, and focal areas of cortical thinning and loss. An aspirate of the right distal femoral lesion yielded mildly cloudy serosanguineous fluid. Cytologic examination of the fluid revealed a pleomorphic population of discrete cells that exhibited marked anisocytosis and anisokaryosis and a variable nuclear‐to‐cytoplasmic (N:C) ratio, which were interpreted as probable neoplastic cells, with few macrophages, and evidence of hemorrhage. Given the clinical signs of pain, lesion size, and concern for malignant neoplasia, amputation of the right hind limb was performed. Histologically, the lesion had undulating walls 1‐3 mm thick with a continuous outer layer of dense fibrous tissue and an inner layer composed of reactive cancellous bone with no cortical compacta remaining. Remnants of thin fibrous or fibro‐osseous septa projected from the bony wall into the cyst lumen. The final histologic diagnosis was a benign multiloculated solitary (unicameral) bone cyst of the distal right femur. Based on the histopathologic findings, it was speculated that the cells identified on cytology were a mixture of developing osteoclasts, osteoblasts, endothelial, and stromal cells. This is the first report describing the cytologic examination of a solitary bone cyst in veterinary medicine.}, number={3}, journal={Veterinary Clinical Pathology}, publisher={Wiley}, author={Roode, Sarah C. and Shive, Heather R. and Hoorntje, Willemijn and Bernard, Jennifer and Stowe, Devorah M. and Pool, Roy R. and Grindem, Carol B.}, year={2018}, month={May}, pages={484–488} } @article{roode_rotroff_richards_moore_motsinger-reif_okamura_mizuno_tsujimoto_suter_breen_2016, title={Comprehensive genomic characterization of five canine lymphoid tumor cell lines}, volume={12}, journal={BMC Veterinary Research}, author={Roode, S. C. and Rotroff, D. and Richards, K. L. and Moore, P. and Motsinger-Reif, A. and Okamura, Y. and Mizuno, T. and Tsujimoto, H. and Suter, S. E. and Breen, M.}, year={2016} } @article{culver_ito_borst_bell_modiano_breen_2013, title={Molecular characterization of canine BCR-ABL-positive chronic myelomonocytic leukemia before and after chemotherapy}, volume={42}, ISSN={0275-6382}, url={http://dx.doi.org/10.1111/vcp.12055}, DOI={10.1111/vcp.12055}, abstractNote={AbstractGenetic aberrations linked to tumorigenesis have been identified in both canine and human hematopoietic malignancies. While the response of human patients to cancer treatments is often evaluated using cytogenetic techniques, this approach has not been used for dogs with comparable neoplasias. The aim of this study was to demonstrate the applicability of cytogenetic techniques to evaluate the cytogenetic response of canine leukemia to chemotherapy. Cytology and flow cytometric techniques were used to diagnose chronic myelomonocytic leukemia in a dog. High‐resolution oligonucleotide array comparative genomic hybridization (oaCGH) and multicolor fluorescence in situ hybridization (FISH) were performed to identify and characterize DNA copy number aberrations (CNAs) and targeted structural chromosome aberrations in peripheral blood WBC at the time of diagnosis and following one week of chemotherapy. At the time of diagnosis, oaCGH indicated the presence of 22 distinct CNAs, of which trisomy of dog chromosome 7 (CFA 7) was the most evident. FISH analysis revealed that this CNA was present in 42% of leukemic cells; in addition, a breakpoint cluster region‐Abelson murine leukemia viral oncogene homolog (BCR‐ABL) translocation was evident in 17.3% of cells. After one week of treatment, the percentage of cells affected by trisomy of CFA7 and BCR‐ABL translocation was reduced to 2% and 3.3%, respectively. Chromosome aberrations in canine leukemic cells may be monitored by molecular cytogenetic techniques to demonstrate cytogenetic remission following treatment. Further understanding of the genetic aberrations involved in canine leukemia may be crucial to improve treatment protocols.}, number={3}, journal={Veterinary Clinical Pathology}, publisher={Wiley}, author={Culver, Sarah and Ito, Daisuke and Borst, Luke and Bell, Jerold S. and Modiano, Jaime F. and Breen, Matthew}, year={2013}, month={Jun}, pages={314–322} } @article{perez_culver_owen_dunbar_kow_breen_milner_2013, title={Partial cytogenetic response with toceranib and prednisone treatment in a young dog with chronic monocytic leukemia}, volume={24}, ISSN={["1473-5741"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84885370605&partnerID=MN8TOARS}, DOI={10.1097/cad.0000000000000018}, abstractNote={Treatment of chronic monocytic leukemia (CMoL) in dogs has traditionally consisted of hydroxyurea. The use of tyrosine kinase inhibitors has been proposed as a treatment option for dogs with CMoL but has never been reported. We report a case of CMoL in a young dog that achieved clinical remission with treatment with the tyrosine kinase inhibitor toceranib and prednisone.}, number={10}, journal={ANTI-CANCER DRUGS}, author={Perez, Mayrim L. and Culver, Sarah and Owen, Jennifer L. and Dunbar, Mark and Kow, Kelvin and Breen, Matthew and Milner, Rowan J.}, year={2013}, month={Nov}, pages={1098–1103} } @article{figueiredo_culver_behling-kelly_breen_friedrichs_2012, title={Acute myeloblastic leukemia with associated BCR-ABL translocation in a dog}, volume={41}, ISSN={["0275-6382"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-84865982358&partnerID=MN8TOARS}, DOI={10.1111/j.1939-165x.2012.00450.x}, abstractNote={AbstractAn 8‐year‐old male neutered Labrador Retriever was referred to the University of Wisconsin Veterinary Medical Teaching Hospital with a presumptive diagnosis of leukemia. Hematologic abnormalities included normal neutrophil count with a left shift, monocytosis, eosinophilia, thrombocytopenia, and circulating immature mononuclear cells. Bone marrow was effaced by immature hematopoietic cells of various morphologic appearances. In addition, large multinucleated cells were observed frequently. Flow cytometric analysis of nucleated cells in blood revealed 34% CD34+ cells, consistent with acute leukemia. By immunocytochemical analysis of cells in blood and bone marrow, some mononuclear cells expressed CD18, myeloperoxidase, and CD11b, indicating myeloid origin; some, but not all, large multinucleated cells expressed CD117 and CD42b, the latter supporting megakaryocytic lineage. The diagnosis was acute myeloblastic leukemia without maturation (AML‐M1). To identify genetic aberrations associated with this malignancy, cells from formalin‐fixed paraffin‐embedded bone marrow were analyzed cytogenetically by multicolor fluorescence in situ hybridization (FISH). Co‐localization of bacterial artificial chromosome (BAC) containing BCR and ABL was evident in 32% of cells. This confirmed the presence of the canine BCR‐ABL translocation or Raleigh chromosome. In people, the analogous translocation or Philadelphia chromosome is characteristic of chronic myelogenous leukemia (CML) and is rarely reported in AML. BCR‐ABL translocation also has been identified in dogs with CML; however, to our knowledge this is the first report of AML with a BCR‐ABL translocation in a domestic animal.}, number={3}, journal={VETERINARY CLINICAL PATHOLOGY}, author={Figueiredo, Josely F. and Culver, Sarah and Behling-Kelly, Erica and Breen, Matthew and Friedrichs, Kristen R.}, year={2012}, month={Sep}, pages={362–368} }