Stefanie Chen

Works (21)

Updated: February 20th, 2026 15:40

2025 article

A 3D-printed Sanger sequencing activity addresses student misconceptions

Brown, P. E., & Chen, S. H. (2025, April 16). (J. L. Hsu, Ed.). Journal of Microbiology and Biology Education, Vol. 4.

By: P. Brown n  & S. Chen n 

Ed(s): J. Hsu

author keywords: Sanger sequencing; misconceptions; 3D printing; model; tactile; hands-on; Universal Design for Learning
topics (OpenAlex): Biomedical and Engineering Education; Problem and Project Based Learning; Innovative Teaching Methods
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
4. Quality Education (OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: April 16, 2025

2025 article

Xylanolytic metabolism is regulated by coordination of transcription factors XynR and XylR in extremely thermophilic Caldicellulosiruptorales

Manesh, M. J. H., Crosby, J. R., Laemthong, T., Bing, R. G., Chen, S. H., Vailionis, J., … Kelly, R. M. (2025, June 4). (H. Atomi, Ed.). Applied and Environmental Microbiology, Vol. 6.

By: M. Manesh  n, J. Crosby n, T. Laemthong n, R. Bing n , S. Chen n , J. Vailionis*, T. Tanwee *, Y. Zhang * ...

Ed(s): H. Atomi

author keywords: <italic>Caldicellulosiruptorales</italic>; hemicellulose; transcriptional regulator; fluorescence polarization; Hill coefficient
topics (OpenAlex): Biofuel production and bioconversion; Microbial Metabolic Engineering and Bioproduction; Enzyme Production and Characterization
Sources: ORCID, Web Of Science, NC State University Libraries
Added: June 4, 2025

2024 article

Building a valuable professional development course: instructor effort vs. perceived student value of activities in an online biotechnology course

Chen, S. H., Dums, J. T., & Hasley, A. O. (2024, November 13). Frontiers in Education, Vol. 9.

By: S. Chen n , J. Dums n & A. Hasley n

author keywords: professional development; career exploration; social cognitive career theory; cognitive information processing; biotechnology; stem; course development; student perspectives
topics (OpenAlex): Innovative Teaching Methods; Experimental Learning in Engineering; Engineering Education and Curriculum Development
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
4. Quality Education (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: December 2, 2024

2024 journal article

Implementation of electronic lab notebooks (ELNs) in science laboratory classes: student response and lessons learned

Discover Education, 3(1).

By: S. Chen n , C. Garcia *, E. Lazear  n, T. Lentz  n, S. Robertson *, Z. Li  n, C. Goller n 

topics (OpenAlex): Research Data Management Practices; Scientific Computing and Data Management; Species Distribution and Climate Change
Sources: ORCID, Crossref, NC State University Libraries
Added: June 15, 2024

2024 article

“R” U ready?: a case study using R to analyze changes in gene expression during evolution

Pomeroy, A. E., Bixler, A., Chen, S. H., Kerr, J. E., Levine, T. D., & Ryder, E. F. (2024, March 22). Frontiers in Education, Vol. 9.

By: A. Pomeroy *, A. Bixler *, S. Chen n , J. Kerr *, T. Levine* & E. Ryder *

author keywords: high-throughput data analysis; R programming; case studies; evolutionary biology; data cleaning; data visualization; summary statistics; reproducibility
topics (OpenAlex): Genetics, Bioinformatics, and Biomedical Research; Genomics and Phylogenetic Studies; Gene expression and cancer classification
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: April 15, 2024

2023 article

Abstract 1931: Innovating Life Sciences Laboratory Training: Molecular Biology Laboratory Education Modules (MBLEMs) as a Model for Advanced Training at Diverse Institutions

Srougi, M., Corbett, A., Garcia, C., Sabaoun, M., Santisteban, M., Sivaraman, V., … Kelly, R. (2023, January 1). Journal of Biological Chemistry, Vol. 299, pp. S260–S260.

By: M. Srougi* , A. Corbett, C. Garcia, M. Sabaoun, M. Santisteban, V. Sivaraman, S. Chen* , C. Goller* , R. Kelly* 

topics (OpenAlex): Biomedical and Engineering Education; Genetics, Bioinformatics, and Biomedical Research
Sources: Web Of Science, NC State University Libraries
Added: September 11, 2023

2023 article

What Do You Find Beautiful about Viruses? A Post-COVID Assessment Strategy

Chen, S. H. (2023, February 14). (M. K. Barker, Ed.). Journal of Microbiology and Biology Education, Vol. 2.

By: S. Chen n 

Ed(s): M. Barker

author keywords: virus; beauty; assessment; student engagement; student well-being
topics (OpenAlex): Innovative Teaching Methods; Problem and Project Based Learning; Biomedical and Engineering Education
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: February 15, 2023

2022 article

Learning lab skills online: Lessons from implementing video‐based instruction for a remote biotechnology lab

Chen, S. H. (2022, September 2). Biochemistry and Molecular Biology Education, Vol. 9.

By: S. Chen n 

author keywords: biochemistry; COVID-19; teaching online; virtual labs
MeSH headings : Humans; Laboratories; Pandemics; COVID-19 / epidemiology; Students; Biotechnology
topics (OpenAlex): Innovative Teaching Methods; Experimental Learning in Engineering; Various Chemistry Research Topics
TL;DR: Student perspectives and assessments were collected on course participants from Fall 2019 and Fall 2020 to compare experiences and outcomes, and analysis of the data shows that the online students appreciated the videos and gained self‐confidence in the procedures, but maintained more misconceptions about the material. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
4. Quality Education (Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: September 4, 2022

2021 journal article

Integrating Bioinformatics Tools into Inquiry-based Molecular Biology Laboratory Education Modules

Frontiers in Education, 6.

By: C. Goller n , M. Srougi n , S. Chen n , L. Schenkman n & R. Kelly n 

Contributors: C. Goller n , M. Srougi n , S. Chen n , L. Schenkman n & R. Kelly n 

author keywords: bioinformatics; science education; molecular biotechnology; software tools; case studies
topics (OpenAlex): Genetics, Bioinformatics, and Biomedical Research; Biomedical and Engineering Education
TL;DR: This new paradigm for biology laboratory education that embraces bioinformatics as a critical component of inquiry-based learning for undergraduate and graduate students representing the life sciences, the physical sciences, and engineering is described. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
4. Quality Education (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 23, 2021

2020 journal article

Integrating research into a molecular cloning course to address the evolving biotechnology landscape

Biochemistry and Molecular Biology Education, 7(1), 115–128.

By: C. Garcia *, I. Chapman *, S. Chen n , E. Lazear *, T. Lentz  n, C. Williams n, J. Dums *, C. Goller n , S. Robertson *

Contributors: C. Garcia *, I. Chapman *, S. Chen n , E. Lazear *, T. Lentz  n, C. Williams n, J. Dums *, C. Goller n , S. Robertson *

author keywords: calcium signaling; cellular biology; cloning; experimental design; inquiry-based laboratory; molecular biology
MeSH headings : Animals; Biotechnology / education; Calcium / metabolism; Cloning, Molecular; Curriculum; Fluorescent Dyes / chemistry; Humans; Proteins / analysis; Research; Students
topics (OpenAlex): Viral Infectious Diseases and Gene Expression in Insects; CRISPR and Genetic Engineering; bioluminescence and chemiluminescence research
TL;DR: This existing molecular cloning course is redesigned to incorporate an independent research project assessing calcium signaling, and the framework described here provides a versatile structure that can be adapted to merge diverse instructor interests with evolving educational needs. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
4. Quality Education (Web of Science; OpenAlex)
Sources: ORCID, Crossref, NC State University Libraries
Added: July 28, 2020

2020 article

Resolving Toxic DNA repair intermediates in every E. coli replication cycle: critical roles for RecG, Uup and RadD

Romero, Z. J., Chen, S. H., Armstrong, T., Wood, E. A., van Oijen, A., Robinson, A., & Cox, M. M. (2020, June 26). Nucleic Acids Research, Vol. 48, pp. 8445–8460.

By: Z. Romero*, S. Chen n , T. Armstrong *, E. Wood *, A. van Oijen *, A. Robinson *, M. Cox *

MeSH headings : ATP-Binding Cassette Transporters / genetics; Adenosine Triphosphatases / genetics; DNA Repair / genetics; DNA Replication / genetics; DNA, Bacterial / genetics; DNA-Binding Proteins / genetics; Escherichia coli / genetics; Escherichia coli Proteins / genetics; Mutation / genetics; Recombination, Genetic / genetics
topics (OpenAlex): DNA Repair Mechanisms; Bacterial Genetics and Biotechnology; DNA and Nucleic Acid Chemistry
TL;DR: It is demonstrated that RecG function is at least partially complemented by another SF2 helicase, RadD, and the results highlight the importance of the RadD protein for general chromosome maintenance and repair and implicate Uup as a new modulator of RecGfunction. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: October 19, 2020

2019 article

Frequent template switching in postreplication gaps: suppression of deleterious consequences by the Escherichia coli Uup and RadD proteins

Romero, Z. J., Armstrong, T. J., Henrikus, S. S., Chen, S. H., Glass, D. J., Ferrazzoli, A. E., … Cox, M. M. (2019, October 17). Nucleic Acids Research, Vol. 48, pp. 212–230.

By: Z. Romero*, T. Armstrong*, S. Henrikus *, S. Chen n , D. Glass *, A. Ferrazzoli*, E. Wood *, S. Chitteni-Pattu* ...

MeSH headings : ATP-Binding Cassette Transporters / deficiency; ATP-Binding Cassette Transporters / genetics; Adenosine Triphosphatases / deficiency; Adenosine Triphosphatases / genetics; Anti-Bacterial Agents / pharmacology; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Ciprofloxacin / pharmacology; DNA Replication; DNA, Bacterial / genetics; DNA, Bacterial / metabolism; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Drug Resistance, Bacterial / genetics; Escherichia coli / drug effects; Escherichia coli / genetics; Escherichia coli / metabolism; Escherichia coli Proteins / genetics; Gene Expression Regulation, Bacterial; Genome, Bacterial; Plasmids / chemistry; Plasmids / metabolism; Replication Origin; Sequence Deletion
topics (OpenAlex): DNA Repair Mechanisms; Bacterial Genetics and Biotechnology; Carcinogens and Genotoxicity Assessment
TL;DR: Uup and RadD are presented as genomic guardians that govern two pathways for resolution of branched DNA intermediates such that potentially deleterious genome rearrangements arising from frequent template switching are averted. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: April 27, 2020

2019 journal article

Harnessing single‐stranded DNA binding protein to explore protein–protein and protein–DNA interactions

Biochemistry and Molecular Biology Education, 48(2), 181–190.

By: S. Chen n  & C. Goller n 

Contributors: S. Chen n  & C. Goller n 

author keywords: ChIP; gamification; interaction; protein-ligand; yeast two-hybrid
MeSH headings : Binding Sites; Blotting, Western; Chromatin Immunoprecipitation; Curriculum; DNA, Single-Stranded / chemistry; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / isolation & purification; Educational Measurement; Female; Humans; Male; Microscopy, Fluorescence; Students; Surveys and Questionnaires
topics (OpenAlex): Educational Games and Gamification; Teaching and Learning Programming; Genetics, Bioinformatics, and Biomedical Research
TL;DR: A series of five experiments based on the robust bacterial single‐stranded DNA binding protein (SSB) to characterize both known and unknown protein–protein and protein–DNA interactions, which indicate a clear increase in both content knowledge and confidence in the topics presented. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: ORCID, Crossref, NC State University Libraries
Added: December 20, 2019

2019 journal article

Shifting Faculty Approaches to Pedagogy through Structured Teaching Postdoc Experiences

Journal of Microbiology & Biology Education, 20(2).

By: S. Chen n  & C. Goller n 

Contributors: S. Chen n  & C. Goller n 

topics (OpenAlex): Innovative Teaching Methods; Biomedical and Engineering Education; Interdisciplinary Research and Collaboration
TL;DR: The benefits of providing pedagogical training at the postdoctoral level are outlined and a framework for structuring an impactful teaching postdoc program is presented. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
4. Quality Education (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: February 27, 2020

2016 article

Escherichia coli RadD Protein Functionally Interacts with the Single-stranded DNA-binding Protein

Chen, S. H., Byrne-Nash, R. T., & Cox, M. M. (2016, August 13). Journal of Biological Chemistry.

By: S. Chen* , R. Byrne-Nash * & M. Cox *

author keywords: ATPase; DNA repair; protein-DNA interaction; protein-protein interaction; radiation biology; SSB; helicase
MeSH headings : Adenosine Triphosphatases / genetics; Adenosine Triphosphatases / metabolism; DNA Damage; DNA, Bacterial / genetics; DNA, Bacterial / metabolism; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Escherichia coli / genetics; Escherichia coli / metabolism; Escherichia coli Proteins / genetics; Escherichia coli Proteins / metabolism; Protein Binding
topics (OpenAlex): DNA Repair Mechanisms; Bacterial Genetics and Biotechnology; CRISPR and Genetic Engineering
TL;DR: The RadD gene was recently identified as having an unspecified role in repair of radiation damage and, more specifically, DNA double-strand breaks, and it is likely that the repair function of RadD is mediated through interaction with SSB at the site of damage. (via Semantic Scholar)
Source: ORCID
Added: September 12, 2019

2015 article

Active displacement of RecA filaments by UvrD translocase activity

Petrova, V., Chen, S. H., Molzberger, E. T., Tomko, E., Chitteni-Pattu, S., Jia, H., … Cox, M. M. (2015, March 30). Nucleic Acids Research.

By: V. Petrova*, S. Chen* , E. Molzberger*, E. Tomko *, S. Chitteni-Pattu*, H. Jia *, Y. Ordabayev *, T. Lohman *, M. Cox *

MeSH headings : Adenosine Triphosphatases / metabolism; Adenosine Triphosphate / metabolism; Binding Sites; DNA / metabolism; DNA Helicases / metabolism; DNA, Single-Stranded / metabolism; Escherichia coli Proteins / metabolism; Rec A Recombinases / antagonists & inhibitors; Rec A Recombinases / chemistry; Rec A Recombinases / metabolism; Rec A Recombinases / ultrastructure; Sequence Deletion
topics (OpenAlex): DNA Repair Mechanisms; Bacterial Genetics and Biotechnology; RNA Interference and Gene Delivery
TL;DR: It is demonstrated that UvrD utilizes an active mechanism to remove RecA from the DNA and may operate in a specialized disassembly mode. (via Semantic Scholar)
Source: ORCID
Added: September 12, 2019

2014 article

Escherichia coli radD (yejH) gene: a novel function involved in radiation resistance and double‐strand break repair

Chen, S. H., Byrne, R. T., Wood, E. A., & Cox, M. M. (2014, November 26). Molecular Microbiology.

By: S. Chen* , R. Byrne*, E. Wood * & M. Cox *

MeSH headings : Adenosine Triphosphatases / chemistry; Adenosine Triphosphatases / genetics; Adenosine Triphosphatases / metabolism; Alleles; Anti-Bacterial Agents / pharmacology; Ciprofloxacin / pharmacology; DNA Repair / genetics; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Escherichia coli / genetics; Escherichia coli / metabolism; Escherichia coli / radiation effects; Escherichia coli Proteins / chemistry; Escherichia coli Proteins / genetics; Escherichia coli Proteins / metabolism; Genes, Bacterial; Inverted Repeat Sequences; Sequence Alignment; Sequence Deletion; Ultraviolet Rays; Zinc Fingers
topics (OpenAlex): DNA Repair Mechanisms; Bacterial Genetics and Biotechnology; Carcinogens and Genotoxicity Assessment
TL;DR: The yejH gene, which exhibits significant homology to the human transcription‐coupled DNA repair gene XPB, is involved in the repair of double‐strand DNA breaks and should be renamed radD, reflecting its role in the DNA repair of radiation damage. (via Semantic Scholar)
Source: ORCID
Added: September 12, 2019

2014 article

Escherichia coli Genes and Pathways Involved in Surviving Extreme Exposure to Ionizing Radiation

Byrne, R. T., Chen, S. H., Wood, E. A., Cabot, E. L., & Cox, M. M. (2014, July 21). Journal of Bacteriology.

By: R. Byrne*, S. Chen* , E. Wood *, E. Cabot* & M. Cox *

MeSH headings : Escherichia coli / genetics; Escherichia coli / metabolism; Escherichia coli / radiation effects; Escherichia coli Proteins / genetics; Escherichia coli Proteins / metabolism; Gene Expression Regulation, Bacterial / radiation effects; Radiation, Ionizing
topics (OpenAlex): DNA Repair Mechanisms; Bacterial Genetics and Biotechnology; Mitochondrial Function and Pathology
TL;DR: The results reinforced the notion that survival after high doses of ionizing radiation does not depend on a single mechanism or process, but instead is multifaceted. (via Semantic Scholar)
Source: ORCID
Added: September 12, 2019

2014 article

Top3α Is Required during the Convergent Migration Step of Double Holliday Junction Dissolution

Chen, S. H., Plank, J. L., Willcox, S., Griffith, J. D., & Hsieh, T.-shih. (2014, January 2). PLoS ONE.

By: S. Chen* , J. Plank *, S. Willcox*, J. Griffith * & T. Hsieh*

MeSH headings : Animals; DNA Helicases / metabolism; DNA Topoisomerases, Type I / metabolism; DNA, Cruciform / metabolism; DNA, Cruciform / ultrastructure; Drosophila; Drosophila Proteins / metabolism; Protein Binding; Substrate Specificity
topics (OpenAlex): Microtubule and mitosis dynamics; DNA Repair Mechanisms; Plant Disease Resistance and Genetics
TL;DR: It is shown that the presence of a topoisomerase is required for Blm to substantially migrate a topologically constrained Holliday junction and when Blm is together with Top3α, the dissolution reaction is processive with no pausing at a partially migrated structure. (via Semantic Scholar)
Source: ORCID
Added: September 12, 2019

2013 article

New Mechanistic and Functional Insights into DNA Topoisomerases

Chen, S. H., Chan, N.-L., & Hsieh, T.-shih. (2013, March 15). Annual Review of Biochemistry.

By: S. Chen* , N. Chan * & T. Hsieh*

author keywords: antibiotics; anticancer drugs; chromosome segregation; DNA cleavage; DNA religation; DNA strand passage; resolution of recombination intermediates
MeSH headings : Antineoplastic Agents / pharmacology; Catalytic Domain; DNA / genetics; DNA / metabolism; DNA Replication; DNA Topoisomerases, Type I / chemistry; DNA Topoisomerases, Type I / genetics; DNA Topoisomerases, Type I / metabolism; DNA Topoisomerases, Type II / chemistry; DNA Topoisomerases, Type II / genetics; DNA Topoisomerases, Type II / metabolism; Humans; Protein Structure, Tertiary
topics (OpenAlex): Cancer therapeutics and mechanisms; Bioactive Compounds and Antitumor Agents; Synthesis and Biological Evaluation
TL;DR: Recent advances in mechanistic insights into topoisomerases are covered, including a DNA helicase capable of modulating the directionality of strand passage, enabling important functions like reannealing denatured DNA and resolving recombination intermediates. (via Semantic Scholar)
Source: ORCID
Added: September 12, 2019

2012 article

Essential Functions of C Terminus of Drosophila Topoisomerase IIIα in Double Holliday Junction Dissolution

Chen, S. H., Wu, C.-H., Plank, J. L., & Hsieh, T.-shih. (2012, April 18). Journal of Biological Chemistry.

By: S. Chen* , C. Wu*, J. Plank * & T. Hsieh*

MeSH headings : Animals; DNA Helicases / genetics; DNA Helicases / metabolism; DNA Topoisomerases, Type I / genetics; DNA Topoisomerases, Type I / metabolism; DNA, Cruciform / genetics; DNA, Cruciform / metabolism; Drosophila Proteins; Drosophila melanogaster; Mutation; Protein Structure, Tertiary
topics (OpenAlex): Cancer therapeutics and mechanisms; DNA Repair Mechanisms; Biochemical and Molecular Research
TL;DR: The data suggest that the Top3α C terminus has an important role in dHJ dissolution (by providing an interaction interface for Blm and DNA, which are critical to the dissolution reaction) and an essential function in vivo. (via Semantic Scholar)
Source: ORCID
Added: September 12, 2019

Employment

Updated: August 1st, 2019 21:18

2019 - present

North Carolina State University Raleigh, NC, US
Teaching Assistant Professor Biological Sciences/Biotechnology

Education

Updated: August 1st, 2019 21:21

2005 - 2012

Duke University School of Medicine Durham, NC, US
PhD Biochemistry

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