Works (17)
Updated: April 22nd, 2024 05:56
2024 journal article
"R" U ready?: a case study using R to analyze changes in gene expression during evolution
FRONTIERS IN EDUCATION, 9.
author keywords: high-throughput data analysis; R programming; case studies; evolutionary biology; data cleaning; data visualization; summary statistics; reproducibility
www.frontiersin.org (publisher PDF)
Sources: Web Of Science, NC State University Libraries
Added: April 15, 2024
2023 article
Innovating Life Sciences Laboratory Training: Molecular Biology Laboratory Education Modules (MBLEMs) as a Model for Advanced Training at Diverse Institutions
JOURNAL OF BIOLOGICAL CHEMISTRY, Vol. 299, pp. S260–S260.
Sources: Web Of Science, NC State University Libraries
Added: September 11, 2023
2023 article
What Do You Find Beautiful about Viruses? A Post-COVID Assessment Strategy
Chen, S. H. (2023, February 14). (M. K. Barker, Ed.). JOURNAL OF MICROBIOLOGY & BIOLOGY EDUCATION, Vol. 2.
Ed(s): M. Barker
author keywords: virus; beauty; assessment; student engagement; student well-being
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: February 15, 2023
2022 article
Learning lab skills online: Lessons from implementing video-based instruction for a remote biotechnology lab
Chen, S. H. (2022, September 2). BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION, Vol. 9.
author keywords: biochemistry; COVID-19; teaching online; virtual labs
MeSH headings : Humans; Laboratories; Pandemics; COVID-19 / epidemiology; Students; Biotechnology
TL;DR:
Student perspectives and assessments were collected on course participants from Fall 2019 and Fall 2020 to compare experiences and outcomes, and analysis of the data shows that the online students appreciated the videos and gained self‐confidence in the procedures, but maintained more misconceptions about the material.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
4. Quality Education
(Web of Science)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: September 4, 2022
2021 journal article
Integrating Bioinformatics Tools into Inquiry-based Molecular Biology Laboratory Education Modules
Frontiers in Education, 6.
author keywords: bioinformatics; science education; molecular biotechnology; software tools; case studies
TL;DR:
This new paradigm for biology laboratory education that embraces bioinformatics as a critical component of inquiry-based learning for undergraduate and graduate students representing the life sciences, the physical sciences, and engineering is described.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 23, 2021
2020 journal article
Frequent template switching in postreplication gaps: suppression of deleterious consequences by the Escherichia coli Uup and RadD proteins
NUCLEIC ACIDS RESEARCH, 48(1), 212–230.
MeSH headings : ATP-Binding Cassette Transporters / deficiency; ATP-Binding Cassette Transporters / genetics; Adenosine Triphosphatases / deficiency; Adenosine Triphosphatases / genetics; Anti-Bacterial Agents / pharmacology; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Ciprofloxacin / pharmacology; DNA Replication; DNA, Bacterial / genetics; DNA, Bacterial / metabolism; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Drug Resistance, Bacterial / genetics; Escherichia coli / drug effects; Escherichia coli / genetics; Escherichia coli / metabolism; Escherichia coli Proteins / genetics; Gene Expression Regulation, Bacterial; Genome, Bacterial; Plasmids / chemistry; Plasmids / metabolism; Replication Origin; Sequence Deletion
TL;DR:
Uup and RadD are presented as genomic guardians that govern two pathways for resolution of branched DNA intermediates such that potentially deleterious genome rearrangements arising from frequent template switching are averted.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: April 27, 2020
2020 journal article
Harnessing single‐stranded DNA binding protein to explore protein–protein and protein–DNA interactions
Biochemistry and Molecular Biology Education, 48(2), 181–190.
author keywords: ChIP; gamification; interaction; protein-ligand; yeast two-hybrid
MeSH headings : Binding Sites; Blotting, Western; Chromatin Immunoprecipitation; Curriculum; DNA, Single-Stranded / chemistry; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / isolation & purification; Educational Measurement; Female; Humans; Male; Microscopy, Fluorescence; Students; Surveys and Questionnaires
TL;DR:
A series of five experiments based on the robust bacterial single‐stranded DNA binding protein (SSB) to characterize both known and unknown protein–protein and protein–DNA interactions, which indicate a clear increase in both content knowledge and confidence in the topics presented.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: ORCID, Crossref, NC State University Libraries
Added: December 20, 2019
2020 journal article
Integrating research into a molecular cloning course to address the evolving biotechnology landscape
Biochemistry and Molecular Biology Education, 7.
author keywords: calcium signaling; cellular biology; cloning; experimental design; inquiry-based laboratory; molecular biology
MeSH headings : Animals; Biotechnology / education; Calcium / metabolism; Cloning, Molecular; Curriculum; Fluorescent Dyes / chemistry; Humans; Proteins / analysis; Research; Students
TL;DR:
This existing molecular cloning course is redesigned to incorporate an independent research project assessing calcium signaling, and the framework described here provides a versatile structure that can be adapted to merge diverse instructor interests with evolving educational needs.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
4. Quality Education
(Web of Science; OpenAlex)
Sources: ORCID, Crossref, NC State University Libraries
Added: July 28, 2020
2020 journal article
Resolving Toxic DNA repair intermediates in every E. coli replication cycle: critical roles for RecG, Uup and RadD
NUCLEIC ACIDS RESEARCH, 48(15), 8445–8460.
MeSH headings : ATP-Binding Cassette Transporters / genetics; Adenosine Triphosphatases / genetics; DNA Repair / genetics; DNA Replication / genetics; DNA, Bacterial / genetics; DNA-Binding Proteins / genetics; Escherichia coli / genetics; Escherichia coli Proteins / genetics; Mutation / genetics; Recombination, Genetic / genetics
TL;DR:
It is demonstrated that RecG function is at least partially complemented by another SF2 helicase, RadD, and the results highlight the importance of the RadD protein for general chromosome maintenance and repair and implicate Uup as a new modulator of RecGfunction.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: October 19, 2020
2019 journal article
Shifting Faculty Approaches to Pedagogy through Structured Teaching Postdoc Experiences
Journal of Microbiology & Biology Education, 20(2).
TL;DR:
The benefits of providing pedagogical training at the postdoctoral level are outlined and a framework for structuring an impactful teaching postdoc program is presented.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
4. Quality Education
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: February 27, 2020
2016 journal article
Escherichia coli RadD Protein Functionally Interacts with the Single-stranded DNA-binding Protein
Journal of Biological Chemistry.
author keywords: ATPase; DNA repair; protein-DNA interaction; protein-protein interaction; radiation biology; SSB; helicase
MeSH headings : Adenosine Triphosphatases / genetics; Adenosine Triphosphatases / metabolism; DNA Damage; DNA, Bacterial / genetics; DNA, Bacterial / metabolism; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Escherichia coli / genetics; Escherichia coli / metabolism; Escherichia coli Proteins / genetics; Escherichia coli Proteins / metabolism; Protein Binding
TL;DR:
The RadD gene was recently identified as having an unspecified role in repair of radiation damage and, more specifically, DNA double-strand breaks, and it is likely that the repair function of RadD is mediated through interaction with SSB at the site of damage.
(via Semantic Scholar)
Source: ORCID
Added: September 12, 2019
2015 journal article
Active displacement of RecA filaments by UvrD translocase activity
Nucleic Acids Research.
MeSH headings : Adenosine Triphosphatases / metabolism; Adenosine Triphosphate / metabolism; Binding Sites; DNA / metabolism; DNA Helicases / metabolism; DNA, Single-Stranded / metabolism; Escherichia coli Proteins / metabolism; Rec A Recombinases / antagonists & inhibitors; Rec A Recombinases / chemistry; Rec A Recombinases / metabolism; Rec A Recombinases / ultrastructure; Sequence Deletion
TL;DR:
It is demonstrated that UvrD utilizes an active mechanism to remove RecA from the DNA and may operate in a specialized disassembly mode.
(via Semantic Scholar)
Source: ORCID
Added: September 12, 2019
2015 journal article
Escherichia coli radD (yejH) gene: a novel function involved in radiation resistance and double-strand break repair
Molecular Microbiology.
MeSH headings : Adenosine Triphosphatases / chemistry; Adenosine Triphosphatases / genetics; Adenosine Triphosphatases / metabolism; Alleles; Anti-Bacterial Agents / pharmacology; Ciprofloxacin / pharmacology; DNA Repair / genetics; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Escherichia coli / genetics; Escherichia coli / metabolism; Escherichia coli / radiation effects; Escherichia coli Proteins / chemistry; Escherichia coli Proteins / genetics; Escherichia coli Proteins / metabolism; Genes, Bacterial; Inverted Repeat Sequences; Sequence Alignment; Sequence Deletion; Ultraviolet Rays; Zinc Fingers
TL;DR:
The yejH gene, which exhibits significant homology to the human transcription‐coupled DNA repair gene XPB, is involved in the repair of double‐strand DNA breaks and should be renamed radD, reflecting its role in the DNA repair of radiation damage.
(via Semantic Scholar)
Source: ORCID
Added: September 12, 2019
2014 journal article
Escherichia coli Genes and Pathways Involved in Surviving Extreme Exposure to Ionizing Radiation
Journal of Bacteriology.
MeSH headings : Escherichia coli / genetics; Escherichia coli / metabolism; Escherichia coli / radiation effects; Escherichia coli Proteins / genetics; Escherichia coli Proteins / metabolism; Gene Expression Regulation, Bacterial / radiation effects; Radiation, Ionizing
TL;DR:
The results reinforced the notion that survival after high doses of ionizing radiation does not depend on a single mechanism or process, but instead is multifaceted.
(via Semantic Scholar)
Source: ORCID
Added: September 12, 2019
2014 journal article
Top3α Is Required during the Convergent Migration Step of Double Holliday Junction Dissolution
PLoS ONE.
MeSH headings : Animals; DNA Helicases / metabolism; DNA Topoisomerases, Type I / metabolism; DNA, Cruciform / metabolism; DNA, Cruciform / ultrastructure; Drosophila; Drosophila Proteins / metabolism; Protein Binding; Substrate Specificity
TL;DR:
It is shown that the presence of a topoisomerase is required for Blm to substantially migrate a topologically constrained Holliday junction and when Blm is together with Top3α, the dissolution reaction is processive with no pausing at a partially migrated structure.
(via Semantic Scholar)
Source: ORCID
Added: September 12, 2019
2013 journal article
New Mechanistic and Functional Insights into DNA Topoisomerases
Annual Review of Biochemistry.
author keywords: antibiotics; anticancer drugs; chromosome segregation; DNA cleavage; DNA religation; DNA strand passage; resolution of recombination intermediates
MeSH headings : Antineoplastic Agents / pharmacology; Catalytic Domain; DNA / genetics; DNA / metabolism; DNA Replication; DNA Topoisomerases, Type I / chemistry; DNA Topoisomerases, Type I / genetics; DNA Topoisomerases, Type I / metabolism; DNA Topoisomerases, Type II / chemistry; DNA Topoisomerases, Type II / genetics; DNA Topoisomerases, Type II / metabolism; Humans; Protein Structure, Tertiary
TL;DR:
Recent advances in mechanistic insights into topoisomerases are covered, including a DNA helicase capable of modulating the directionality of strand passage, enabling important functions like reannealing denatured DNA and resolving recombination intermediates.
(via Semantic Scholar)
Source: ORCID
Added: September 12, 2019
2012 journal article
Essential Functions of C Terminus of Drosophila Topoisomerase IIIα in Double Holliday Junction Dissolution
Journal of Biological Chemistry.
MeSH headings : Animals; DNA Helicases / genetics; DNA Helicases / metabolism; DNA Topoisomerases, Type I / genetics; DNA Topoisomerases, Type I / metabolism; DNA, Cruciform / genetics; DNA, Cruciform / metabolism; Drosophila Proteins; Drosophila melanogaster; Mutation; Protein Structure, Tertiary
TL;DR:
The data suggest that the Top3α C terminus has an important role in dHJ dissolution (by providing an interaction interface for Blm and DNA, which are critical to the dissolution reaction) and an essential function in vivo.
(via Semantic Scholar)
Source: ORCID
Added: September 12, 2019
Employment
Updated: August 1st, 2019 21:18
2019 - present
North Carolina State University Raleigh, NC, US
Teaching Assistant Professor
Biological Sciences/Biotechnology
Education
Updated: August 1st, 2019 21:21
2005 - 2012
Duke University School of Medicine Durham, NC, US
PhD
Biochemistry
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