Shan M. Randall

Works (8)

Updated: July 5th, 2023 15:46

2016 journal article

Composition of Rosenthal Fibers, the Protein Aggregate Hallmark of Alexander Disease

JOURNAL OF PROTEOME RESEARCH, 15(7), 2265–2282.

By: M. Heaven, D. Flint, S. Randall n, A. Sosunov, L. Wilson, S. Barnes, J. Goldman, D. Muddiman n , M. Brenner

author keywords: Rosenthal fiber; Alexander disease; protein aggregates; neurodegenerative disease; astrocyte; proteomics; mass spectrometry (MS); stress granules
MeSH headings : Alexander Disease; Animals; Astrocytes; Cyclin D2 / analysis; DEAD-box RNA Helicases / analysis; GTP-Binding Proteins / analysis; Humans; Immunohistochemistry; Mice; Neoplasm Proteins / analysis; Neuropeptides / analysis; Protein Aggregates; Protein Aggregation, Pathological; Proteome / analysis; RNA Helicases / analysis; Receptors for Activated C Kinase; Receptors, Cell Surface / analysis
TL;DR: No support was obtained for the sequestration hypothesis for AxD, but the association of several of the RF proteins with stress granules suggests a role for stressgranules in the origin of RFs. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

Identification of Epigenetic Factor Proteins Expressed in Human Embryonic Stem Cell-Derived Trophoblasts and in Human Placental Trophoblasts

JOURNAL OF PROTEOME RESEARCH, 15(8), 2433–2444.

By: P. Sarkar n, A. Mischler n, S. Randall n, T. Collier n, K. Dorman*, K. Boggess*, D. Muddiman n , B. Rao n

author keywords: quantitative mass spectrometry; SILAC; human embryonic stem cell; trophoblast; epigenetic factor
MeSH headings : Cell Differentiation; DNA (Cytosine-5-)-Methyltransferases / genetics; Epigenomics; Female; Gene Expression / genetics; Human Embryonic Stem Cells / cytology; Humans; Placenta / chemistry; Placenta / cytology; Pregnancy; Transcription Factors / genetics; Trophoblasts / chemistry; Trophoblasts / cytology
TL;DR: The results support the use of hESC-derived trophoblasts as a model for placental troPHoblasts, which will enable further investigation of epigenetic factors involved in human trophoblast development, and hypothesized that nuclear proteomics of h ESPCs may be used for screening epigenetic factor proteins expressed by primary trophblasts in human placental tissue. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Evaluating nonpolar surface area and liquid chromatography/mass spectrometry response: an application for site occupancy measurements for enzyme intermediates in polyketide biosynthesis

Rapid Communications in Mass Spectrometry, 28(23), 2511–2522.

By: S. Randall n, I. Koryakina n, G. Williams n  & D. Muddiman n 

MeSH headings : Acyltransferases / metabolism; Chromatography, Liquid / methods; Filtration; Hydrophobic and Hydrophilic Interactions; Mass Spectrometry / methods; Oligopeptides / chemistry; Oligopeptides / metabolism; Peptide Fragments / chemistry; Peptide Fragments / metabolism; Polyketides / chemistry; Polyketides / metabolism
TL;DR: The MS signal as a function of nonpolar surface area (NPSA) is explored in order to better understand this bias in electrospray response and to determine relative LC/MS response for peptides. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2013 journal article

Accurate Identification of Deamidated Peptides in Global Proteomics Using a Quadrupole Orbitrap Mass Spectrometer

JOURNAL OF PROTEOME RESEARCH, 13(2), 777–785.

By: A. Nepomuceno n, R. Gibson n, S. Randall n & D. Muddiman n 

author keywords: deamidation; post-translational modification; quadrupole orbitrap mass spectrometry
MeSH headings : Amides / chemistry; Chromatography, Liquid; Peptides / chemistry; Proteomics; Tandem Mass Spectrometry / instrumentation
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

Factorial Experimental Designs Elucidate Significant Variables Affecting Data Acquisition on a Quadrupole Orbitrap Mass Spectrometer

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 24(10), 1501–1512.

By: S. Randall n, H. Cardasis* & D. Muddiman n 

author keywords: Design of experiments; Data dependent acquisition; Proteome coverage; Q Exactive; FTMS
MeSH headings : Models, Chemical; Proteome / analysis; Proteome / chemistry; Proteomics / methods; Research Design; Saccharomyces cerevisiae Proteins / analysis; Saccharomyces cerevisiae Proteins / chemistry; Tandem Mass Spectrometry / methods
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Targeted Proteomics of the Secretory Pathway Reveals the Secretome of Mouse Embryonic Fibroblasts and Human Embryonic Stem Cells

MOLECULAR & CELLULAR PROTEOMICS, 11(12), 1829–1839.

By: P. Sarkar n, S. Randall n, D. Muddiman n  & B. Rao n

MeSH headings : Animals; Cell Differentiation; Cells, Cultured; Culture Media, Conditioned / analysis; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Fibroblasts / cytology; Fibroblasts / metabolism; Humans; Mice; Proteins / analysis; Secretory Pathway; Subcellular Fractions / chemistry
TL;DR: Targeted proteomics of secretory pathway organelles is shown to be a powerful alternate approach for interrogating the cellular secretome and expected to be particularly valuable in two contexts highly relevant to hESC biology: obtaining a temporal snapshot of proteins secreted in response to a differentiation trigger, and identifying proteinssecreted by cells that are isolated from a heterogeneous population. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Comparison of stable-isotope labeling with amino acids in cell culture and spectral counting for relative quantification of protein expression

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 25(17), 2524–2532.

By: T. Collier n, S. Randall n, P. Sarkar n, B. Rao n, R. Dean n  & D. Muddiman n 

MeSH headings : Amino Acid Sequence; Amino Acids / chemistry; Amino Acids / metabolism; Animals; Cell Differentiation; Cell Line; Ectoderm; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Humans; Isotope Labeling / methods; Mass Spectrometry; Mice; Molecular Sequence Data; Peptide Fragments; Peptide Mapping; Proteins / analysis; Proteins / chemistry; Proteins / metabolism; Proteomics / methods; Sensitivity and Specificity; Tandem Mass Spectrometry; Time Factors
TL;DR: The comparison of stable-isotope labeling of amino acids in cell culture (SILAC) with spectral counting for the quantification of human embryonic stem cells as they differentiate toward the trophectoderm at three time points is presented. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

The subcellular proteome of undifferentiated human embryonic stem cells

PROTEOMICS, 12(3), 421–430.

By: P. Sarkar n, T. Collier n, S. Randall n, D. Muddiman n  & B. Rao n

author keywords: Cell biology; Extracellular proteins; Human embryonic stem cells; Subcellular fractionation; Subcellular proteomics
MeSH headings : Animals; Cell Differentiation; Cells, Cultured; Culture Media, Conditioned; Cytosol / metabolism; Embryonic Stem Cells / cytology; Embryonic Stem Cells / metabolism; Endoplasmic Reticulum / genetics; Endoplasmic Reticulum / metabolism; Gene Expression Regulation, Developmental; Golgi Apparatus / genetics; Golgi Apparatus / metabolism; Humans; Membrane Proteins / genetics; Membrane Proteins / metabolism; Mice; Nuclear Proteins / genetics; Nuclear Proteins / metabolism; Signal Transduction / genetics; Subcellular Fractions / metabolism
TL;DR: The results show that systematic subcellular proteome analysis is a valuable tool for studying hESC biology and shows that proteomic analysis of the endoplasmic reticulum and Golgi compartments is a powerful alternative approach to identify secreted proteins since these are synthesized in the ER and transit through the Golgi. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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