Steven Nagar

Works (17)

Updated: April 5th, 2024 01:23

2016 journal article

Evaluation of the proliferative capacity of canine retinal pigment epithelial cells harvested from different regions of the fundus

Investigative Ophthalmology and Visual Science, 57(12).

By: F. Mowat, J. Hash, P. Mzyk, J. Harned, S. Nagar & M. McGahan

Source: NC State University Libraries
Added: August 6, 2018

2014 journal article

Hypoxia controls iron metabolism and glutamate secretion in retinal pigmented epithelial cells

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1840(10), 3138–3144.

By: J. Harned n, S. Nagar n & M. McGahan n

author keywords: Iron transport; Glutamate; Hypoxia; Blood-ocular barriers
MeSH headings : Animals; Cell Hypoxia; Cells, Cultured; Dogs; Epithelial Cells / metabolism; Epithelial Cells / pathology; Glutamic Acid / metabolism; Humans; Iron / metabolism; Oxidation-Reduction; Retinal Pigment Epithelium / metabolism; Retinal Pigment Epithelium / pathology; Stroke / metabolism; Stroke / pathology
TL;DR: Iron, a key catalyst of free radical generation, is removed from RPE under hypoxic conditions which may help protect RPE from oxidative stress and indicate the importance of using polarized tight junctional cells as more physiologically relevant models for blood-barrier-like systems. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Source-Dependent Intracellular Distribution of Iron in Lens Epithelial Cells Cultured Under Normoxic and Hypoxic Conditions

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 54(12), 7666–7673.

By: M. Goralska n, S. Nagar*, L. Fleisher*, P. Mzyk* & M. McGahan

author keywords: iron trafficking; transferrin; NTBI; lens epithelial cells; hypoxia
MeSH headings : Animals; Biological Transport; Cell Nucleus / metabolism; Cell Proliferation; Cell Survival; Cells, Cultured; Chlorides / metabolism; Dogs; Epithelial Cells / metabolism; Ferric Compounds / metabolism; Hypoxia / metabolism; Intracellular Fluid / metabolism; Iron Radioisotopes / metabolism; Lens, Crystalline / cytology; Lens, Crystalline / metabolism; Mitochondria / metabolism; Transferrin / metabolism
TL;DR: Uptake and distribution of NTBI are not as strictly regulated as that of iron from transferrin, and source-dependent differences in iron uptake and trafficking are found. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Ceruloplasmin alters intracellular iron regulated proteins and pathways: Ferritin, transferrin receptor, glutamate and hypoxia-inducible factor-1α

Experimental Eye Research, 97(1), 90–97.

By: J. Harned n, J. Ferrell n, S. Nagar n, M. Goralska n, L. Fleisher n & M. McGahan n

author keywords: ceruloplasmin; iron; lens epithelial cells; retinal pigmented epithelial cells glutamate; hypoxia-inducible factor; transferrin receptor; ferritin
MeSH headings : Animals; Cells, Cultured; Ceruloplasmin / metabolism; Ceruloplasmin / pharmacology; Dogs; Epithelial Cells / drug effects; Epithelial Cells / metabolism; Ferritins / metabolism; Fluorescent Antibody Technique, Indirect; Glutamic Acid / metabolism; Hypoxia-Inducible Factor 1, alpha Subunit / metabolism; Iron Chelating Agents / pharmacology; Lens, Crystalline / cytology; Oxalates / pharmacology; Receptors, Transferrin / metabolism; Retinal Pigment Epithelium / drug effects; Retinal Pigment Epithelium / metabolism
TL;DR: The Cp increased production and secretion of the neurotransmitter, glutamate, is a substantive finding of clinical relevance because of the neural and retinal degeneration found in aceruloplasminemia patients. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref
Added: August 6, 2018

2010 journal article

Altered Ferritin Subunit Composition: Change in Iron Metabolism in Lens Epithelial Cells and Downstream Effects on Glutathione Levels and VEGF Secretion

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 51(9), 4437–4446.

By: J. Harned n, J. Ferrell n, M. Lall n, L. Fleisher n, S. Nagar n, M. Goralska n, M. McGahan n

MeSH headings : Animals; Apoferritins / genetics; Apoferritins / metabolism; Cell Nucleus / metabolism; Cells, Cultured; Culture Media, Conditioned / pharmacology; Cystine / pharmacokinetics; Dogs; Epithelial Cells / cytology; Epithelial Cells / metabolism; Glutathione / metabolism; Homeostasis / physiology; Hypoxia-Inducible Factor 1, alpha Subunit / metabolism; Iron / metabolism; Lens, Crystalline / cytology; Lens, Crystalline / metabolism; Oxidative Stress / physiology; Protein Subunits / genetics; Protein Subunits / metabolism; RNA, Small Interfering; Transfection; Vascular Endothelial Growth Factor A / metabolism
TL;DR: Iron storage in ferritin was greatly reduced by FHsiRNA, resulting in increased iron availability, as noted by a decrease in transferrin receptor levels and iron uptake from transferrin, and profound effects clearly demonstrate that maintenance of a specific H:L ratio is part of a basic cellular homeostatic mechanism. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Changes in Ferritin H- and L-Chains in Canine Lenses with Age-Related Nuclear Cataract

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 50(1), 305–310.

By: M. Goralska n, S. Nagar n, C. Colitz n, L. Fleisher n & M. McGahan n

MeSH headings : Aging; Animals; Apoferritins / metabolism; Cataract / metabolism; Cataract / veterinary; Dog Diseases / metabolism; Dogs; Electrophoresis, Polyacrylamide Gel / veterinary; Enzyme-Linked Immunosorbent Assay / veterinary; Fluorescent Antibody Technique, Indirect / veterinary; Lens, Crystalline / metabolism; Microscopy, Fluorescence / veterinary
TL;DR: There are significant differences in the characteristics of ferritin H-chain and its distribution in canine cataractous lenses compared with noncataractsous lenses, which allows this molecule to sequester more iron. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Distribution of ferritin chains in canine lenses with and without age-related nuclear cataracts

Molecular Vision, 15(256-59), 2404–2410.

By: M. Goralska, S. Nagar, L. Fleisher & M. McGahan

Source: NC State University Libraries
Added: August 6, 2018

2009 journal article

Iron metabolism in the eye: A review

Experimental Eye Research, 88(2), 204–215.

By: M. Goralska n, J. Ferrell n, J. Harned n, M. Lall n, S. Nagar n, L. Fleisher n, M. McGahan n

author keywords: iron; ferritin; transferrin; lens; retinal pigmented epithelium; retina
MeSH headings : Animals; Eye / metabolism; Eye Diseases / metabolism; Homeostasis; Humans; Iron / metabolism; Oxidative Stress; Signal Transduction / physiology
TL;DR: This review article covers all aspects of iron metabolism, which include studies of iron levels within the eye and the processes used to maintain normal levels of iron in ocular tissues. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, Crossref
Added: August 6, 2018

2008 journal article

Iron regulates L-cystine uptake and glutathione levels in lens epithelial and retinal pigment epithelial cells by its effect on cytosolic aconitase

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 49(1), 310–319.

By: M. Lall n, J. Ferrell n, S. Nagar n, L. Fleisher n & M. McGahan n

MeSH headings : 2,2'-Dipyridyl / pharmacology; Aconitate Hydratase / antagonists & inhibitors; Aconitate Hydratase / metabolism; Amino Acid Transport System y+ / genetics; Amino Acid Transport System y+ / metabolism; Animals; Cells, Cultured; Chelating Agents / pharmacology; Cystine / metabolism; Cytosol; Dogs; Enzyme Inhibitors / pharmacology; Epithelial Cells / drug effects; Epithelial Cells / metabolism; Fluorescent Antibody Technique, Indirect; Gene Expression; Glutathione / metabolism; Immunoblotting; Lens, Crystalline / drug effects; Lens, Crystalline / metabolism; Oxalates / pharmacology; Pigment Epithelium of Eye / metabolism; RNA, Messenger / metabolism; Reverse Transcriptase Polymerase Chain Reaction; Transferrin / pharmacology
TL;DR: The authors found that the iron-induced increase in L-glutamate availability increased L-cystine uptake, with subsequent increases in GSH levels, demonstrating for the first time that iron regulates L-cycline uptake and the downstream production of GSH in two mammalian cell types. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Differential degradation of ferritin H- and L-chains: Accumulation of L-chain-rich ferritin in lens epithelial cells

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 46(10), 3521–3529.

By: M. Goralska n, S. Nagar n, L. Fleisher n & M. McGahan n

MeSH headings : Aging / physiology; Animals; Apoferritins; Cells, Cultured; Cysteine Proteinase Inhibitors / pharmacology; Dogs; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Epithelial Cells / drug effects; Epithelial Cells / metabolism; Ferritins / metabolism; Iron / metabolism; Lens, Crystalline / drug effects; Lens, Crystalline / metabolism; Lysosomes / metabolism; Methionine / metabolism; Plasmids; Transfection
TL;DR: The formation of inclusion bodies in older lens epithelial cells, as demonstrated in the current investigation, is intriguing and could point to possible involvement of cytoplasmic L-chain-enriched ferritin aggregates in the formation of age-related cataract. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Host DNA replication is induced by geminivirus infection of differentiated plant cells

PLANT CELL, 14(12), 2995–3007.

By: S. Nagara, L. Hanley-Bowdoin n  & D. Robertson n

MeSH headings : Aphidicolin / pharmacology; Bromodeoxyuridine / metabolism; Cell Differentiation / genetics; Cell Differentiation / physiology; DNA Replication / drug effects; DNA Replication / genetics; DNA Replication / physiology; DNA, Viral / drug effects; DNA, Viral / metabolism; Geminiviridae / genetics; Geminiviridae / physiology; Hydroxyurea / pharmacology; Immunohistochemistry; In Situ Hybridization; Microscopy, Fluorescence; Tobacco / chemistry; Tobacco / genetics; Tobacco / virology
TL;DR: 5-bromo-2-deoxyuridine incorporation to examine DNA synthesis directly in infected Nicotiana benthamiana plants to determine if viral reprogramming of host replication controls had an impact on host DNA replication and demonstrate that chromosomal DNA is replicated in TGMV-infected cells. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2000 journal article

A geminivirus replication protein interacts with the retinoblastoma protein through a novel domain to determine symptoms and tissue specificity of infection in plants

EMBO JOURNAL, 19(13), 3485–3495.

By: L. Kong n, B. Orozco, J. Roe, S. Nagar, S. Ou, H. Feiler, T. Durfee, A. Miller ...

author keywords: AL1; cell cycle; differentiation; plant DNA virus; pRb
MeSH headings : Antigens, Polyomavirus Transforming / metabolism; Base Sequence; DNA Primers; Geminiviridae / isolation & purification; Geminiviridae / metabolism; Geminiviridae / physiology; Plants / virology; Protein Binding; Retinoblastoma Protein / metabolism; Viral Proteins / metabolism; Virus Replication
TL;DR: The pRBR binding domain of AL1 is mapped between amino acids 101 and 180 and two mutants are identified that are differentially impacted for AL1–pRBR interactions during geminivirus infection of plants. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2000 journal article

Chromosome condensation induced by geminivirus infection of mature plant cells

Journal of Cell Science, 113(7), 1149–1160.

By: H. Bass, S. Nagar, L. Hanley-Bowdoin  & D. Robertson

Source: NC State University Libraries
Added: August 6, 2018

2000 journal article

Geminiviruses - Models for plant DNA replication, transcription and cell cycle regulation ([correction to] vol 35, pg 105, 2000)

Critical Reviews in Biochemistry and Molecular Biology, 35(4), U4.

By: L. Hanley-Bowdoin , S. Settlage, B. Orozco, S. Nagar & D. Robertson

Source: NC State University Libraries
Added: August 6, 2018

2000 journal article

Geminiviruses: Models for plant DNA replication, transcription, and cell cycle regulation

Critical Reviews in Biochemistry and Molecular Biology, 35(2), 105–140.

By: L. Hanley-Bowdoin , S. Settlage, B. Orozco, S. Nagar & D. Robertson

Source: NC State University Libraries
Added: August 6, 2018

1999 journal article

Geminiviruses: Models for plant DNA replication, transcription, and cell cycle regulation {review}

Critical Reviews in Plant Sciences, 18(1), 71–106.

By: L. Hanley-Bowdoin n , S. Settlage n, B. Orozco n, S. Nagar n & D. Robertson n

UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: NC State University Libraries
Added: August 6, 2018

1995 journal article

A geminivirus induces expression of a host DNA synthesis protein in terminally differentiated plant cells

Plant Cell, 7(6), 705.

By: S. Nagar*, T. Pedersen, K. Carrick, L. Hanley-Bowdoin & D. Robertson

Source: NC State University Libraries
Added: August 6, 2018

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