@article{liu_xu_amanullah_du_che_zhang_jiang_zhu_wang_2024, title={Deciphering the Enhancing Impact of Exogenous Brassinolide on Physiological Indices of Melon Plants under Downy Mildew-Induced Stress}, url={https://doi.org/10.3390/plants13060779}, DOI={10.3390/plants13060779}, abstractNote={Melon (Cucumis melo L.) is a valuable horticultural crop of the Cucurbitaceae family. Downy mildew (DM), caused by Pseudoperonospora cubensis, is a significant inhibitor of the production and quality of melon. Brassinolide (BR) is a new type of phytohormone widely used in cultivation for its broad spectrum of resistance- and defense-mechanism-improving activity. In this study, we applied various exogenous treatments (0.5, 1.0, and 2.0 mg·L−1) of BR at four distinct time periods (6 h, 12 h, 24 h, and 48 h) and explored the impact of BR on physiological indices and the genetic regulation of melon seedling leaves infected by downy-mildew-induced stress. It was mainly observed that a 2.0 mg·L−1 BR concentration effectively promoted the enhanced photosynthetic activity of seedling leaves, and quantitative real-time polymerase chain reaction (qRT-PCR) analysis similarly exhibited an upregulated expression of the predicted regulatory genes of photosystem II (PSII) CmHCF136 (MELO3C023596.2) and CmPsbY (MELO3C010708.2), thus indicating the stability of the PSII reaction center. Furthermore, 2.0 mg·L−1 BR resulted in more photosynthetic pigments (nearly three times more than the chlorophyll contents (264.52%)) as compared to the control and other treatment groups and similarly upregulated the expression trend of the predicted key enzyme genes CmLHCP (MELO3C004214.2) and CmCHLP (MELO3C017176.2) involved in chlorophyll biosynthesis. Meanwhile, the maximum contents of soluble sugars and starch (186.95% and 164.28%) were also maintained, which were similarly triggered by the upregulated expression of the predicted genes CmGlgC (MELO3C006552.2), CmSPS (MELO3C020357.2), and CmPEPC (MELO3C018724.2), thereby maintaining osmotic adjustment and efficiency in eliminating reactive oxygen species. Overall, the exogenous 2.0 mg·L−1 BR exhibited maintained antioxidant activities, plastid membranal stability, and malondialdehyde (MDA) content. The chlorophyll fluorescence parameter values of F0 (42.23%) and Fv/Fm (36.67%) were also noticed to be higher; however, nearly three times higher levels of NPQ (375.86%) and Y (NPQ) (287.10%) were observed at 48 h of treatment as compared to all other group treatments. Increased Rubisco activity was also observed (62.89%), which suggested a significant role for elevated carbon fixation and assimilation and the upregulated expression of regulatory genes linked with Rubisco activity and the PSII reaction process. In short, we deduced that the 2.0 mg·L−1 BR application has an enhancing effect on the genetic modulation of physiological indices of melon plants against downy mildew disease stress.}, journal={Plants}, author={Liu, Tai and Xu, Huichun and Amanullah, Sikandar and Du, Zhiqiang and Che, Ye and Zhang, Ling and Jiang, Zeyu and Zhu, Lei and Wang, Di}, year={2024}, month={Mar} }
 @article{yang_amanullah_li_gao_bai_li_ma_luan_wang_2024, title={Deciphering the Genomic Characterization of the GGP Gene Family and Expression Verification of CmGGP1 Modulating Ascorbic Acid Biosynthesis in Melon Plants}, url={https://doi.org/10.3390/antiox13040397}, DOI={10.3390/antiox13040397}, abstractNote={Ascorbic acid (AsA), also known as vitamin C, is a well-known antioxidant found in living entities that plays an essential role in growth and development, as well as in defensive mechanisms. GDP-L-galactose phosphorylase (GGP) is a candidate gene regulating AsA biosynthesis at the translational and transcriptional levels in plants. In the current study, we conducted genome-wide bioinformatic analysis and pinpointed a single AsA synthesis rate-limiting enzyme gene in melon (CmGGP1). The protein prediction analysis depicted that the CmGGP1 protein does not have a signaling peptide or transmembrane structure and mainly functions in the chloroplast or nucleus. The constructed phylogenetic tree analysis in multispecies showed that the CmGGP1 protein has a highly conserved motif in cucurbit crops. The structural variation analysis of the CmGGP1 gene in different domesticated melon germplasms showed a single non-synonymous type-base mutation and indicated that this gene was selected by domestication during evolution. Wild-type (WT) and landrace (LDR) germplasms of melon depicted close relationships to each other, and improved-type (IMP) varieties showed modern domestication selection. The endogenous quantification of AsA content in both the young and old leaves of nine melon varieties exhibited the major differentiations for AsA synthesis and metabolism. The real-time quantitative polymerase chain reaction (qRT-PCR) analysis of gene co-expression showed that AsA biosynthesis in leaves was greater than AsA metabolic consumption, and four putative interactive genes (MELO3C025552.2, MELO3C007440.2, MELO3C023324.2, and MELO3C018576.2) associated with the CmGGP1 gene were revealed. Meanwhile, the CmGGP1 gene expression pattern was noticed to be up-regulated to varying degrees in different acclimated melons. We believe that the obtained results would provide useful insights for an in-depth genetic understanding of the AsA biosynthesis mechanism, aimed at the development of improving crop plants for melon.}, journal={Antioxidants}, author={Yang, Tiantian and Amanullah, Sikandar and Li, Shenglong and Gao, Peng and Bai, Junyu and Li, Chang and Ma, Jie and Luan, Feishi and Wang, Xuezheng}, year={2024}, month={Mar} }
 @article{yang_amanullah_duan_guo_xu_bao_an_yuan_liu_liu_et al._2024, title={Fine genetic mapping and transcriptomic analysis revealed major gene modulating the clear stripe margin pattern of watermelon peel}, volume={15}, ISSN={["1664-462X"]}, url={http://dx.doi.org/10.3389/fpls.2024.1462141}, DOI={10.3389/fpls.2024.1462141}, abstractNote={The peel stripe margin pattern is one of the most important quality traits of watermelon. In this study, two contrasted watermelon lines [slb line (P}, journal={FRONTIERS IN PLANT SCIENCE}, author={Yang, Shao and Amanullah, Sikandar and Duan, Yaru and Guo, Yu and Xu, Ming and Bao, Xiuping and An, Bohan and Yuan, Chengzhi and Liu, Xiujie and Liu, Jixiu and et al.}, year={2024}, month={Sep} }
 @article{a recessive gene <i>cmpmr2f</i> confers powdery mildew resistance in melon (cucumis melo l.) (vol 136, 4, 2023)_2023, url={https://publons.com/wos-op/publon/68299263/}, DOI={10.1007/S00122-023-04324-Y}, journal={Theoretical And Applied Genetics}, year={2023} }
 @article{zhang_cui_luan_liu_ding_amanullah_zhang_ma_gao_2023, title={A recessive gene Cmpmr2F confers powdery mildew resistance in melon (Cucumis melo L.)}, volume={136}, url={http://dx.doi.org/10.1007/s00122-023-04269-2}, DOI={10.1007/s00122-023-04269-2}, number={1}, journal={Theoretical and Applied Genetics}, publisher={Springer Science and Business Media LLC}, author={Zhang, Taifeng and Cui, Haonan and Luan, Feishi and Liu, Hongyu and Ding, Zhuo and Amanullah, Sikandar and Zhang, Manlin and Ma, Tingting and Gao, Peng}, year={2023}, month={Jan}, pages={1–16} }
 @article{duan_li_amanullah_bao_guo_liu_xu_liu_gao_yuan_et al._2023, title={A single nucleotide mutation in ClphyB gene is associated with a short lateral branch phenotype in watermelon}, volume={321}, url={http://dx.doi.org/10.1016/j.scienta.2023.112378}, DOI={10.1016/j.scienta.2023.112378}, journal={Scientia Horticulturae}, publisher={Elsevier BV}, author={Duan, Yaru and Li, Hewei and Amanullah, Sikandar and Bao, Xiuping and Guo, Yu and Liu, Xiujie and Xu, Hongguo and Liu, Jixiu and Gao, Yue and Yuan, Chengzhi and et al.}, year={2023}, month={Nov}, pages={112378} }
 @article{xu_gao_amanullah_guo_bao_duan_liu_liu_gao_luan_2023, title={Fine genetic mapping confers a major gene controlling leaf shape variation in watermelon}, volume={219}, url={http://dx.doi.org/10.1007/s10681-023-03222-0}, DOI={10.1007/s10681-023-03222-0}, number={9}, journal={Euphytica}, publisher={Springer Science and Business Media LLC}, author={Xu, Ming and Gao, Meiling and Amanullah, Sikandar and Guo, Yu and Bao, Xiuping and Duan, Yaru and Liu, Xiujie and Liu, Jixiu and Gao, Yue and Luan, Feishi}, year={2023}, month={Aug} }
 @article{zhang_xu_amanullah_gao_2023, title={Genome-wide identification, evolution, and expression analysis of MLO gene family in melon (Cucumis melo L.)}, volume={14}, url={http://dx.doi.org/10.3389/fpls.2023.1144317}, DOI={10.3389/fpls.2023.1144317}, abstractNote={Powdery mildew (PM) is one of the main fungal diseases that appear during the cultivation of the melon fruit crop. Mildew Resistance Locus "O" (MLO) is known as a gene family and has seven conserved transmembrane domains. An induced functional loss of a specific MLO gene could mainly confer PM resistance to melons. However, the genomic structure of MLO genes and its main role in PM resistance still remain unclear in melon. In this study, bioinformatic analysis identified a total of 14 MLO gene family members in the melon genome sequence, and these genes were distributed in an uneven manner on eight chromosomes. The phylogenetic analysis divided the CmMLO genes into five different clades, and gene structural analysis showed that genes in the same clade had similar intron and exon distribution patterns. In addition, by cloning the CmMLO gene sequence in four melon lines, analyzing the CmMLO gene expression pattern after infection, and making microscopic observations of the infection pattern of PM, we concluded that the CmMLO5 (MELO3C012438) gene plays a negative role in regulating PM-resistance in the susceptible melon line (Topmark), and the critical time point for gene function was noticed at 24 and 72 hours after PM infection. The mutational analysis exhibited a single base mutation at 572 bp, which further results in loss of protein function, thus conferring PM resistance in melon. In summary, our research evidence provides a thorough understanding of the CmMLO gene family and demonstrates their potential role in disease resistance, as well as a theoretical foundation for melon disease resistance breeding.}, journal={Frontiers in Plant Science}, publisher={Frontiers Media SA}, author={Zhang, Taifeng and Xu, Nan and Amanullah, Sikandar and Gao, Peng}, year={2023}, month={Feb} }
 @article{yang_amanullah_li_cheng_zhang_zhao_liu_luan_wang_2023, title={Molecular Mapping of Putative Genomic Regions Controlling Fruit and Seed Morphology of Watermelon}, url={https://www.mdpi.com/1422-0067/24/21/15755}, DOI={10.3390/ijms242115755}, abstractNote={The genetic regulatory basis of qualitative and quantitative phenotypes of watermelon is being investigated in different types of molecular and genetic breeding studies around the world. In this study, biparental F2 mapping populations were developed over two experimental years, and the collected datasets of fruit and seed traits exhibited highly significant correlations. Whole-genome resequencing of comparative parental lines was performed and detected single nucleotide polymorphism (SNP) loci were converted into cleaved amplified polymorphic sequence (CAPS) markers. The screened polymorphic markers were genotyped in segregating populations and two genetic linkage maps were constructed, which covered a total of 2834.28 and 2721.45 centimorgan (cM) genetic lengths, respectively. A total of 22 quantitative trait loci (QTLs) for seven phenotypic traits were mapped; among them, five stable and major-effect QTLs (PC-8-1, SL-9-1, SWi-9-1, SSi-9-1, and SW-6-1) and four minor-effect QTLs (PC-2-1 and PC-2-2; PT-2-1 and PT-2-2; SL-6-1 and SSi-6-2; and SWi-6-1 and SWi-6-2) were observed with 3.77-38.98% PVE. The adjacent QTL markers showed a good fit marker-trait association, and a significant allele-specific contribution was also noticed for genetic inheritance of traits. Further, a total of four candidate genes (Cla97C09G179150, Cla97C09G179350, Cla97C09G180040, and Cla97C09G180100) were spotted in the stable colocalized QTLs of seed size linked traits (SL-9-1 and SWi-9-1) that showed non-synonymous type mutations. The gene expression trends indicated that the seed morphology had been formed in the early developmental stage and showed the genetic regulation of seed shape formation. Hence, we think that our identified QTLs and genes would provide powerful genetic insights for marker-assisted breeding aimed at improving the quality traits of watermelon.}, journal={International Journal of Molecular Sciences}, author={Yang, Tiantian and Amanullah, Sikandar and Li, Shenglong and Cheng, Rui and Zhang, Chen and Zhao, Zhengxiang and Liu, Hongyu and Luan, Feishi and Wang, Xuezheng}, year={2023}, month={Oct} }
 @article{amanullah_li_osae_yang_abbas_gao_wang_liu_gao_luan_2023, title={Primary mapping of quantitative trait loci regulating multivariate horticultural phenotypes of watermelon (Citrullus lanatus L.)}, volume={13}, url={http://dx.doi.org/10.3389/fpls.2022.1034952}, DOI={10.3389/fpls.2022.1034952}, abstractNote={Watermelon fruits exhibit a remarkable diversity of important horticultural phenotypes. In this study, we initiated a primary quantitative trait loci (QTL) mapping to identify the candidate regions controlling the ovary, fruit, and seed phenotypes. Whole genome sequencing (WGS) was carried out for two differentiated watermelon lines, and 350 Mb (96%) and 354 Mb (97%) of re-sequenced reads covered the reference de novo genome assembly, individually. A total of 45.53% non-synonymous single nucleotide polymorphism (nsSNPs) and 54.47% synonymous SNPs (sSNPs) were spotted, which produced 210 sets of novel SNP-based cleaved amplified polymorphism sequence (CAPS) markers by depicting 46.25% co-dominant polymorphism among parent lines and offspring. A biparental F2:3 mapping population comprised of 100 families was used for trait phenotyping and CAPS genotyping, respectively. The constructed genetic map spanned a total of 2,398.40 centimorgans (cM) in length and averaged 11.42 cM, with 95.99% genome collinearity. A total of 33 QTLs were identified at different genetic positions across the eight chromosomes of watermelon (Chr-01, Chr-02, Chr-04, Chr-05, Chr-06, Chr-07, Chr-10, and Chr-11); among them, eight QTLs of the ovary, sixteen QTLs of the fruit, and nine QTLs of the seed related phenotypes were classified with 5.32-25.99% phenotypic variance explained (PVE). However, twenty-four QTLs were identified as major-effect and nine QTLs were mapped as minor-effect QTLs across the flanking regions of CAPS markers. Some QTLs were exhibited as tightly localized across the nearby genetic regions and explained the pleiotropic effects of multigenic nature. The flanking QTL markers also depicted significant allele specific contributions and accountable genes were predicted for respective traits. Gene Ontology (GO) functional enrichment was categorized in molecular function (MF), cellular components (CC), and biological process (BP); however, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were classified into three main classes of metabolism, genetic information processing, and brite hierarchies. The principal component analysis (PCA) of multivariate phenotypes widely demonstrated the major variability, consistent with the identified QTL regions. In short, we assumed that our identified QTL regions provide valuable genetic insights regarding the watermelon phenotypes and fine genetic mapping could be used to confirm them.}, number={1034952}, journal={Frontiers in Plant Science}, author={Amanullah, Sikandar and Li, Shenglong and Osae, Benjamin Agyei and Yang, Tiantian and Abbas, Farhat and Gao, Meiling and Wang, Xuezheng and Liu, Hongyu and Gao, Peng and Luan, Feishi}, year={2023}, month={Jan} }
 @article{liu_amanullah_xu_gao_du_han_che_zhang_qi_wang_2023, title={RNA-Seq Identified Putative Genes Conferring Photosynthesis and Root Development of Melon under Salt Stress}, url={https://www.mdpi.com/2073-4425/14/9/1728}, DOI={10.3390/genes14091728}, abstractNote={Melon is an important fruit crop of the Cucurbitaceae family that is being cultivated over a large area in China. Unfortunately, salt stress has crucial effects on crop plants and damages photosynthesis, membranal lipid components, and hormonal metabolism, which leads to metabolic imbalance and retarded growth. Herein, we performed RNA-seq analysis and a physiological parameter evaluation to assess the salt-induced stress impact on photosynthesis and root development activity in melon. The endogenous quantification analysis showed that the significant oxidative damage in the membranal system resulted in an increased ratio of non-bilayer/bilayer lipid (MGDG/DGDG), suggesting severe irregular stability in the photosynthetic membrane. Meanwhile, root development was slowed down by a superoxidized membrane system, and downregulated genes showed significant contributions to cell wall biosynthesis and IAA metabolism. The comparative transcriptomic analysis also exhibited that major DEGs were more common in the intrinsic membrane component, photosynthesis, and metabolism. These are all processes that are usually involved in negative responses. Further, the WGCN analysis revealed the involvement of two main network modules: the thylakoid membrane and proteins related to photosystem II. The qRT-PCR analysis exhibited that two key genes (MELO3C006053.2 and MELO3C023596.2) had significant variations in expression profiling at different time intervals of salt stress treatments (0, 6, 12, 24, and 48 h), which were also consistent with the RNA-seq results, denoting the significant accuracy of molecular dataset analysis. In summary, we performed an extensive molecular and metabolic investigation to check the salt-stress-induced physiological changes in melon and proposed that the PSII reaction centre may likely be the primary stress target.}, journal={Genes}, author={Liu, Tai and Amanullah, Sikandar and Xu, Huichun and Gao, Peng and Du, Zhiqiang and Han, Mo and Che, Ye and Zhang, Ling and Qi, Guochao and Wang, Di}, year={2023}, month={Aug} }
 @article{osae_amanullah_liu_liu_saroj_zhang_liu_gao_luan_2022, title={CAPS marker-base genetic linkage mapping and QTL analysis for watermelon ovary, fruit and seed-related traits}, volume={218}, url={http://dx.doi.org/10.1007/s10681-022-02990-5}, DOI={10.1007/s10681-022-02990-5}, number={4}, journal={Euphytica}, publisher={Springer Science and Business Media LLC}, author={Osae, Benjamin Agyei and Amanullah, Sikandar and Liu, Hongyu and Liu, Shi and Saroj, Arvind and Zhang, Chen and Liu, Tai and Gao, Peng and Luan, Feishi}, year={2022}, month={Apr} }
 @article{lv_amanullah_liu_zhang_liu_zhu_zhang_gao_luan_2022, title={Comparative Transcriptome Analysis Identified Key Pathways and Genes Regulating Differentiated Stigma Color in Melon (Cucumis melo L.)}, volume={23}, url={https://doi.org/10.3390/ijms23126721}, DOI={10.3390/ijms23126721}, abstractNote={Stigma color is an important morphological trait in many flowering plants. Visual observations in different field experiments have shown that a green stigma in melons is more attractive to natural pollinators than a yellow one. In the current study, we evaluated the characterization of two contrasted melon lines (MR-1 with a green stigma and M4-7 with a yellow stigma). Endogenous quantification showed that the chlorophyll and carotenoid content in the MR-1 stigmas was higher compared to the M4-7 stigmas. The primary differences in the chloroplast ultrastructure at different developmental stages depicted that the stigmas of both melon lines were mainly enriched with granum, plastoglobulus, and starch grains. Further, comparative transcriptomic analysis was performed to identify the candidate pathways and genes regulating melon stigma color during key developmental stages (S1-S3). The obtained results indicated similar biological processes involved in the three stages, but major differences were observed in light reactions and chloroplast pathways. The weighted gene co-expression network analysis (WGCNA) of differentially expressed genes (DEGs) uncovered a "black" network module (655 out of 5302 genes), mainly corresponding to light reactions, light harvesting, the chlorophyll metabolic process, and the chlorophyll biosynthetic process, and exhibited a significant contribution to stigma color. Overall, the expression of five key genes of the chlorophyll synthesis pathway-CAO (MELO03C010624), CHLH (MELO03C007233), CRD (MELO03C026802), HEMA (MELO03C011113), POR (MELO03C016714)-were checked at different stages of stigma development in both melon lines using quantitative real time polymerase chain reaction (qRT-PCR). The results exhibited that the expression of these genes gradually increased during the stigma development of the MR-1 line but decreased in the M4-7 line at S2. In addition, the expression trends in different stages were the same as RNA-seq, indicating data accuracy. To sum up, our research reveals an in-depth molecular mechanism of stigma coloration and suggests that chlorophyll and related biological activity play an important role in differentiating melon stigma color.}, number={12}, journal={International Journal of Molecular Sciences}, publisher={MDPI AG}, author={Lv, Yuanzuo and Amanullah, Sikandar and Liu, Shi and Zhang, Chen and Liu, Hongyu and Zhu, Zicheng and Zhang, Xian and Gao, Peng and Luan, Feishi}, year={2022}, month={Jun}, pages={6721} }
 @article{amanullah_osae_yang_li_abbas_liu_liu_song_wang_gao_et al._2022, title={Development of Whole Genome SNP-CAPS Markers and Preliminary QTL Mapping of Fruit Pedicel Traits in Watermelon}, volume={13}, url={http://dx.doi.org/10.3389/fpls.2022.879919}, DOI={10.3389/fpls.2022.879919}, abstractNote={Fruit pedicel (FP) is an important determinant of premium fruit quality that directly affects commercial market value. However, in-depth molecular and genetic basis of pedicel-related traits has not been identified in watermelon. Herein, a quantitative trait locus (QTL) mapping strategy was used to identify the potential genetic regions controlling FP traits based on newly derived whole-genome single nucleotide polymorphism based cleaved amplified polymorphism sequence (SNP-CAPS) markers. Next-generation sequencing based whole-genome re-sequencing of two watermelon parent lines revealed 98.30 and 98.40% of average coverage, 4,989,869 SNP variants, and 182,949 CAPS loci pairs across the reference genome, respectively. A total of 221 sets of codominant markers exhibited 46.42% polymorphism rate and were effectively genotyped within 100-F2:3 derived mapping population. The developed linkage map covered a total of 2,630.49 cM genetic length with averaged 11.90 cM, and depicted a valid marker-trait association. In total, 6 QTLs (qFPL4.1, qFPW4.1, qFPD2.1, qFPD2.2, qFPD8.1, qFPD10.1) were mapped with five major effects and one minor effect between the whole genome adjacent markers positioned over distinct chromosomes (02, 04, 08, 10), based on the ICIM-ADD mapping approach. These significant QTLs were similarly mapped in delimited flanking regions of 675.10, 751.38, 859.24, 948.39, and 947.51 kb, which collectively explained 8.64-13.60% PVE, respectively. A highly significant and positive correlation was found among the observed variables. To our knowledge, we first time reported the mapped QTLs/genes affecting FP traits of watermelon, and our illustrated outcomes will deliver the potential insights for fine genetic mapping as well as functional gene analysis through MAS-based breeding approaches.}, journal={Frontiers in Plant Science}, publisher={Frontiers Media SA}, author={Amanullah, Sikandar and Osae, Benjamin Agyei and Yang, Tiantian and Li, Shenglong and Abbas, Farhat and Liu, Shi and Liu, Shusen and Song, Zhengfeng and Wang, Xuezheng and Gao, Peng and et al.}, year={2022}, month={May} }
 @article{kanu_ashraf_mansaray_abbas_fiaz_amanullah_charley_tang_2022, title={Exogenous Methyl Jasmonate Application Improved Physio-Biochemical Attributes, Yield, Quality, and Cadmium Tolerance in Fragrant Rice}, volume={13}, url={http://dx.doi.org/10.3389/fpls.2022.849477}, DOI={10.3389/fpls.2022.849477}, abstractNote={Cadmium (Cd) has detrimental effects on crop plants, whereas, jasmonates (JAs) play a vital role in abiotic stress tolerance in plants. The present study investigated the effects of exogenous application of methyl jasmonate (MeJa) on the physio-biochemical attributes, yield, and quality of two fragrant rice cultivars, i.e., Xiangyaxiangzhan and Meixiangzhan-2 under Cd stress. The experiment was comprised of four treatments, i.e., CK, control (normal conditions); Cd: 100 mg Cd kg-1 of soil; MeJa: exogenous application of MeJa at 20 mM; and Cd + MeJa: 100 mg Cd kg-1 of soil + exogenous MeJa application at 20 mM. Results depicted that Cd toxicity resulted in a substantial reduction of enzymatic activities and non-enzymatic antioxidants, chlorophyll contents, while enhanced oxidative damage in the terms of lipid peroxidation (higher malondialdehyde (MDA) contents), H2O2, and electrolyte leakage. Proline contents were found higher whereas protein and soluble sugars were lower under Cd stress as compared with Ck and Cd + MeJa. Exogenous MeJa application further improved the panicles per pot, spikelets per panicle, seed setting (%), 1,000 grain weight, and yield per pot under Cd stress conditions as compared with non-MeJa applied plant under Cd stress. In addition, exogenous MeJa application enhanced the accumulation of macro (N, P, K, Mg, and Ca) and micronutrients (Mn, Zn, Fe, and Cr) in both cultivars under Cd stress, while reduced the Cd contents in different plant parts. Overall, the contents of Cd in different plant organs were recorded as: root > stem > leaves > grains for all treatments. Comparing both cultivars, the grain Cd contents were higher in Meixiangzhan 2 than Xiangyaxianzhan under Cd contaminated conditions. Conclusively, Cd toxicity impaired growth in rice by affecting physio-biochemical attributes, however, Xiangyaxiangzhan performed better than Meixiangzhan-2 cultivar.}, journal={Frontiers in Plant Science}, publisher={Frontiers Media SA}, author={Kanu, Adam Sheka and Ashraf, Umair and Mansaray, Lamin R. and Abbas, Farhat and Fiaz, Sajid and Amanullah, Sikandar and Charley, Christen Shaka and Tang, Xiangru}, year={2022}, month={Apr} }
 @article{lv_gao_liu_fang_zhang_liu_amanullah_wang_luan_2022, title={Genetic Mapping and QTL Analysis of Stigma Color in Melon (Cucumis melo L.)}, volume={13}, url={http://dx.doi.org/10.3389/fpls.2022.865082}, DOI={10.3389/fpls.2022.865082}, abstractNote={Melon is an important Cucurbitaceae crop. Field observations had shown that the green stigmas of melon are more attractive to pollinators than yellow stigmas. In this study, F 2 and F 2:3 populations obtained by crossing MR-1 (green stigma) and M4-7 (yellow stigma) were used for genetic analysis and mapping. A genetic map of 1,802.49 cm was constructed with 116 cleaved amplified polymorphism sequence (CAPS) markers. Two stable quantitative trait loci (QTLs) linked to the trait of stigma color were identified on chromosomes 2 ( SC2.1 ) and 8 ( SC8.1 ), respectively. An expanded F 2 population was used to narrow down the confidence regions of SC2.1 and SC8.1 . As a result, SC2.1 was further mapped to a 3.6 cm region between CAPS markers S2M3 and S2B1-3, explaining 9.40% phenotypic variation. SC8.1 was mapped to a 3.7-cm region between CAPS markers S8E7 and S8H-1, explaining 25.92% phenotypic variation. This study broadens our understanding of the mechanisms of stigma color regulation and will be of benefit to the breeding of melon.}, journal={Frontiers in Plant Science}, publisher={Frontiers Media SA}, author={Lv, Yuanzuo and Gao, Peng and Liu, Shi and Fang, Xufeng and Zhang, Taifeng and Liu, Tai and Amanullah, Sikandar and Wang, Xinying and Luan, Feishi}, year={2022}, month={May} }
 @article{yang_zhang_pan_amanullah_luan_han_liu_wang_2022, title={Genome-Wide Analysis of the Peroxidase Gene Family and Verification of Lignin Synthesis-Related Genes in Watermelon}, url={https://www.mdpi.com/1422-0067/23/2/642}, DOI={10.3390/ijms23020642}, abstractNote={Watermelon (Citrullus lanatus) is an important horticultural crop worldwide, but peel cracking caused by peel hardness severely decreases its quality. Lignification is one of the important functions of class III peroxidase (PRX), and its accumulation in the plant cell wall leads to cell thickening and wood hardening. For in-depth physiological and genetical understanding, we studied the relationship between peel hardness and lignin accumulation and the role of PRXs affecting peel lignin biosynthesis using genome-wide bioinformatics analysis. The obtained results showed that lignin accumulation gradually increased to form the peel stone cell structure, and tissue lignification led to peel hardness. A total of 79 ClPRXs (class III) were identified using bioinformatics analysis, which were widely distributed on 11 chromosomes. The constructed phylogenetics indicated that ClPRXs were divided into seven groups and eleven subclasses, and gene members of each group had highly conserved intron structures. Repeated pattern analysis showed that deletion and replication events occurred during the process of ClPRX amplification. However, in the whole-protein sequence alignment analysis, high homology was not observed, although all contained four conserved functional sites. Repeated pattern analysis showed that deletion and replication events occurred during ClPRXs' amplification process. The prediction of the promoter cis-acting element and qRT-PCR analysis in four tissues (leaf, petiole, stem, and peel) showed different expression patterns for tissue specificity, abiotic stress, and hormone response by providing a genetic basis of the ClPRX gene family involved in a variety of physiological processes in plants. To our knowledge, we for the first time report the key roles of two ClPRXs in watermelon peel lignin synthesis. In conclusion, the extensive data collected in this study can be used for additional functional analysis of ClPRXs in watermelon growth and development and hormone and abiotic stress response.}, journal={International Journal of Molecular Sciences}, author={Yang, Tiantian and Zhang, Pengyu and Pan, Jiahui and Amanullah, Sikandar and Luan, Feishi and Han, Wenhao and Liu, Hongyu and Wang, Xuezheng}, year={2022}, month={Jan} }
 @article{liang_gao_amanullah_guo_liu_xu_liu_gao_yuan_luan_2022, title={Identification of QTLs linked with watermelon fruit and seed traits using GBS-based high-resolution genetic mapping}, volume={303}, url={http://dx.doi.org/10.1016/j.scienta.2022.111237}, DOI={10.1016/j.scienta.2022.111237}, journal={Scientia Horticulturae}, publisher={Elsevier BV}, author={Liang, Xiaoxue and Gao, Meiling and Amanullah, Sikandar and Guo, Yu and Liu, Xiujie and Xu, Hongguo and Liu, Jixiu and Gao, Yue and Yuan, Chengzhi and Luan, Feishi}, year={2022}, month={Sep}, pages={111237} }
 @article{amanullah_osae_yang_abbas_liu_liu_wang_gao_luan_2022, title={Mapping of genetic loci controlling fruit linked morphological traits of melon using developed CAPS markers}, volume={3}, url={http://dx.doi.org/10.1007/s11033-022-07263-x}, DOI={10.1007/s11033-022-07263-x}, journal={Molecular Biology Reports}, publisher={Springer Science and Business Media LLC}, author={Amanullah, Sikandar and Osae, Benjamin Agyei and Yang, Tiantian and Abbas, Farhat and Liu, Shi and Liu, Hongyu and Wang, Xuezheng and Gao, Peng and Luan, Feishi}, year={2022}, month={Mar} }
 @article{abbas_guo_zhou_wu_amanullah_wang_shen_2022, title={Metabolome and transcriptome analysis of terpene synthase genes and their putative role in floral aroma production in
            <i>Litchi chinensis</i>}, volume={174}, url={http://dx.doi.org/10.1111/ppl.13796}, DOI={10.1111/ppl.13796}, abstractNote={Volatile organic compounds (VOCs) are essential traits of flowers since they attract pollinators, aid in seed distribution, protect the plant from internal and external stimuli, and are involved in plant-plant and plant-environment interactions. Apart from their role in plants, VOCs are used in pharmaceuticals, fragrances, cosmetics, and flavorings. Litchi (Litchi chinensis Sonn.) is a popular fruit due to its enticing red appearance, exotic taste, and high nutritional qualities. Litchi flowers bloom as inflorescences primarily on the shoot terminals. There are three distinct flower types, two male and one female, all of which are produced on the same panicle and rely on insect pollination. Herein, we used a comprehensive metabolomic approach to examine the volatile profile of litchi fruit (green pericarp, yellow pericarp, and red pericarp) as well as male and female flowers (bud stage, half open and full bloom). From a quantitative examination of the volatiles in L. chinensis, a total of 19, 22, and 21 VOCs were discovered from female flowers, male flowers, and fruits, with the majority of them belonging to sesquiterpenes. Multivariate analysis revealed that the volatile profiles of fruits differ from those of male and female flowers. Three VOCs were unique to male flowers and ten to the fruit, while eight VOCs were shared by both male and female flowers and eleven by both male and female flowers and the fruit. Furthermore, for the first time, we identified and comprehensively studied the TERPENE SYNTHASE genes (TPS) using the litchi genome and transcriptome database, which revealed 38 TPS genes unevenly distributed across the 15 chromosomes. A phylogenetic study showed that LcTPS were grouped into TPS-b, TPS-c, TPS-e, TPS-f, and TPS-g subfamilies, with TPS-b having the most genes. The conserved motifs (RRX8 W, NSE/DTE, and DDXX D) were studied in LcTPSs, and significant variation between subfamilies was discovered. Furthermore, after integrating the metabolome and transcriptome datasets, several VOCs were shown to be development-specific and highly linked with distinct LcTPS genes, making them promising biomarkers. Interestingly, LcTPS17/20/23/24/31 were associated with monoterpene edges, while the rest were connected to sesquiterpene edges, indicating their probable participation in the aroma biosynthesis mechanism of certain compounds.}, number={6}, journal={Physiologia Plantarum}, publisher={Wiley}, author={Abbas, Farhat and Guo, Shaoying and Zhou, Yiwei and Wu, Jing and Amanullah, Sikandar and Wang, Hui-Cong and Shen, Jiyuan}, year={2022}, month={Nov} }
 @article{huang_abbas_rothenberg_imran_fiaz_rehman_amanullah_younas_ding_cai_et al._2022, title={Molecular cloning, characterization and expression analysis of two 12-oxophytodienoate reductases (NtOPR1 and NtOPR2) from Nicotiana tabacum}, volume={2}, url={http://dx.doi.org/10.1007/s11033-022-07114-9}, DOI={10.1007/s11033-022-07114-9}, journal={Molecular Biology Reports}, publisher={Springer Science and Business Media LLC}, author={Huang, Feiyan and Abbas, Farhat and Rothenberg, Dylan O’Neill and Imran, Muhammad and Fiaz, Sajid and Rehman, Naveed and Amanullah, Sikandar and Younas, Afifa and Ding, Yan and Cai, Xianjie and et al.}, year={2022}, month={Feb} }
 @article{liang_gao_amanullah_guo_xu_liu_liu_liu_gao_yuan_et al._2022, title={Molecular mapping of candidate gene regulating fruit stripe trait in watermelon}, volume={218}, url={http://dx.doi.org/10.1007/s10681-022-03128-3}, DOI={10.1007/s10681-022-03128-3}, number={12}, journal={Euphytica}, publisher={Springer Science and Business Media LLC}, author={Liang, Xiaoxue and Gao, Meiling and Amanullah, Sikandar and Guo, Yu and Xu, Hongguo and Liu, Xiaosong and Liu, Xiujie and Liu, Jixiu and Gao, Yue and Yuan, Chengzhi and et al.}, year={2022}, month={Dec} }
 @article{study on the cultivation of seedlings using buds of potato (solanum tuberosum l.)_2022, journal={PeerJ}, year={2022}, month={Sep} }
 @article{yang_liu_li_amanullah_lu_zhang_zhang_luan_liu_wang_2022, title={Transcriptomic Analysis of Fusarium oxysporum Stress-Induced Pathosystem and Screening of Fom-2 Interaction Factors in Contrasted Melon Plants}, volume={13}, url={http://dx.doi.org/10.3389/fpls.2022.961586}, DOI={10.3389/fpls.2022.961586}, abstractNote={Fusarium wilt is one of the most destructive and less controllable diseases in melon, which is usually caused by fusarium oxysporum . In this study, transcriptome sequencing and Yeast Two-Hybrid (Y2H) methods were used for quantification of differentially expressed genes (DEGs) involved in fusarium oxysporum (f. sp. melonis race 1) stress-induced mechanisms in contrasted melon varieties (M4-45 “susceptible” and MR-1 “resistant”). The interaction factors of Fom-2 resistance genes were also explored in response to the plant-pathogen infection mechanism. Transcriptomic analysis exhibited total 1,904 new genes; however, candidate DEGs analysis revealed a total of 144 specific genes (50 upregulated and 94 downregulated) for M4-45 variety and 104 specific genes (71 upregulated and 33 downregulated) for MR-1 variety, respectively. The analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway depicted some candidate DEGs, including Phenylalanine metabolism, phenylpropane biosynthesis, plants-pathogen interaction, and signal transduction of plant hormones, which were mainly involved in disease resistance metabolic pathways. The weighted gene co-expression network analysis (WGCNA) analysis revealed a strong correlation module and exhibited the disease resistance-related genes encoding course proteins, transcription factors, protein kinase, benzene propane biosynthesis path, plants-pathogen interaction pathway, and glutathione S-transferase. Meanwhile, the resistance-related specific genes expression was relatively abundant in MR-1 compared to the M4-45, and cell wall-associated receptor kinases ( MELO3C008452 and MELO3C008453 ), heat shock protein (Cucumis_melo_newGene_172), defensin-like protein (Cucumis_melo_newGene_5490), and disease resistance response protein ( MELO3C016325 ), activator response protein ( MELO3C021623 ), leucine-rich repeat receptor protein kinase ( MELO3C024412 ), lactyl glutathione ligase (Cucumis_melo_newGene_36), and unknown protein ( MELO3C007588 ) were persisted by exhibiting the upregulated expressions. At the transcription level, the interaction factors between the candidate genes in response to the fusarium oxysporum induced stress, and Y2H screening signified the main contribution of MYB transcription factors ( MELO3C009678 and MELO3C014597 ), BZIP ( MELO3C011839 and MELO3C019349 ), unknown proteins, and key enzymes in the ubiquitination process ( 4XM334FK014 ). The candidate genes were further verified in exogenously treated melon plants with f. oxysporum ( Fom-2 , Race 1), Abscisic acid (ABA), Methyl Jasmonite (MeJA), and Salicylic acid (SA), using the fluorescence quantitative polymerase chain reaction (qRT-PCR) analysis. The overall expression results indicated that the SA signal pathway is involved in effective regulation of the Fom-2 gene activity.}, journal={Frontiers in Plant Science}, publisher={Frontiers Media SA}, author={Yang, Tiantian and Liu, Jiajun and Li, Xiaomei and Amanullah, Sikandar and Lu, Xueyan and Zhang, Mingchong and Zhang, Yanhang and Luan, Feishi and Liu, Hongyu and Wang, Xuezheng}, year={2022}, month={Jul} }
 @article{assessment on seed oil percentage and physicochemical properties of watermelon (<i>citrullus lanatus</i>)_2021, url={https://publons.com/wos-op/publon/70483058/}, journal={Rivista Italiana Delle Sostanze Grasse}, year={2021} }
 @article{assessment on seed oil percentege and physicochemical properties of watermelon (citrullus lanatus)_2021, journal={Rivista Italiana Delle Sostanze Grasse}, year={2021} }
 @article{zhang_liu_amanullah_ding_cui_luan_gao_2021, title={Fine Mapping of Cla015407 Controlling Plant Height in Watermelon}, volume={146}, url={http://dx.doi.org/10.21273/jashs04934-20}, DOI={10.21273/jashs04934-20}, abstractNote={The plant compact and dwarf growth habit is an important agronomic trait when breeding watermelon ( Citrullus lanatus ) cultivars because of their reduced vine length, high-density planting, and better land utilization; however, the genetic basis of the dwarf growth habit is not well-known. In this study, the plant population of six generations, P1, P2, F1, F2, BC1P1, and BC1P2, were studied. A genetic segregation analysis demonstrated that dwarfism is mainly controlled by a single recessive Cldw gene. Furthermore, whole-genome sequencing of two distinct watermelon cultivars, W1-1 (P1) and 812 (P2), was performed and preliminarily mapped through a bulked segregant analysis of F2 individuals that revealed the Cldw gene locus on chromosome 9. Two candidate genes, Cla015407 and Cla015408 , were discovered at the delimited region of 43.2 kb by fine mapping, and gene annotation exposed that the Cla015407 gene encodes gibberellic acid 3β-hydroxylase protein. In addition, a comparative analysis of gene sequence and cultivars sequences across the reference genome of watermelon revealed the splice site mutation in the intron region of the Cldw gene in dwarf-type cultivar 812. The quantitative real-time polymerase chain reaction exhibited a significantly higher expression of the Cla015407 gene in cultivar W1-1 compared with 812. There was no significant difference in the vine length of both cultivars after gibberellic acid treatment. In brief, our fine mapping demonstrated that Cla015407 is a candidate gene controlling dwarfism of watermelon plants.}, number={3}, journal={Journal of the American Society for Horticultural Science}, publisher={American Society for Horticultural Science}, author={Zhang, Taifeng and Liu, Jiajun and Amanullah, Sikandar and Ding, Zhuo and Cui, Haonan and Luan, Feishi and Gao, Peng}, year={2021}, month={May}, pages={196–205} }
 @article{abbas_ke_zhou_yu_imran_amanullah_rothenberg_wang_wang_fan_2021, title={Functional Characterization of Hedychium coronarium J. Koenig MYB132 Confers the Potential Role in Floral Aroma Synthesis}, volume={10}, url={http://dx.doi.org/10.3390/plants10102014}, DOI={10.3390/plants10102014}, abstractNote={The R2R3-MYB transcription factors (TFs) play several key roles in numerous plant biological processes.}, number={10}, journal={Plants}, publisher={MDPI AG}, author={Abbas, Farhat and Ke, Yanguo and Zhou, Yiwei and Yu, Rangcai and Imran, Muhammad and Amanullah, Sikandar and Rothenberg, Dylan O’Neill and Wang, Qin and Wang, Lan and Fan, Yanping}, year={2021}, month={Sep}, pages={2014} }
 @article{yang_amanullah_pan_chen_liu_ma_wang_gao_wang_2021, title={Identification of putative genetic regions for watermelon rind hardness and related traits by BSA-seq and QTL mapping}, volume={217}, url={http://dx.doi.org/10.1007/s10681-020-02758-9}, DOI={10.1007/s10681-020-02758-9}, number={2}, journal={Euphytica}, publisher={Springer Science and Business Media LLC}, author={Yang, Tiantian and Amanullah, Sikandar and Pan, Jiahui and Chen, Guixiang and Liu, Shi and Ma, Shuangwu and Wang, Jiming and Gao, Peng and Wang, Xuezheng}, year={2021}, month={Feb} }
 @article{antioxidant and anti-inflammatory capacities of pepper tissues_2020, url={https://doi.org/10.14674/IJFS-1700}, DOI={10.14674/IJFS-1700}, abstractNote={The objective of this study was to investigate the antioxidant and anti-inflammatory activities of five pepper varieties tissues. Green Bell peppers had the highest total antioxidant contents; while Red Chilli variety had the lowest antioxidant activities (ABTS was 3.89 ?mol TE/g fw, DPPH was 2.82 ?mol TE/g fw and FRAP was 16.95 ?mol TE/g fw). The methanolic extracts of different peppers showed strong but different anti-inflammatory activity values (8.22 ?g/ml - 9.52 ?g). Yellow Bell, Red and Green Chilli had the highest anti-inflammatory activity followed by Green and Red Bell extracts, respectively. The results suggest that these varieties of pepper could contribute as sources of important antioxidant and anti-inflammatory related to the oxidative stress and inflammation prevention.}, journal={Italian Journal of Food Science}, year={2020}, month={May} }
 @article{rokayya_jia_li_nie_xu_han_yu_amanullah_almatrafi_helal_2021, title={Application of nano-titanum dioxide coating on fresh Highbush blueberries shelf life stored under ambient temperature}, volume={137}, url={http://dx.doi.org/10.1016/j.lwt.2020.110422}, DOI={10.1016/j.lwt.2020.110422}, journal={LWT}, publisher={Elsevier BV}, author={Rokayya, Sami and Jia, Fuguo and Li, Yang and Nie, Xin and Xu, Jingwen and Han, Rui and Yu, Huiying and Amanullah, Sikandar and Almatrafi, Manal Mused and Helal, Mahmoud}, year={2021}, month={Feb}, pages={110422} }
 @article{amanullah_saroj_osae_liu_liu_gao_luan_2020, title={Detection of putative QTL regions associated with ovary traits in melon using SNP-CAPS markers}, volume={270}, url={https://doi.org/10.1016/j.scienta.2020.109445}, DOI={10.1016/j.scienta.2020.109445}, journal={Scientia Horticulturae}, publisher={Elsevier BV}, author={Amanullah, Sikandar and Saroj, Arvind and Osae, Benjamin Agyei and Liu, Shi and Liu, Hongyu and Gao, Peng and Luan, Feishi}, year={2020}, month={Aug}, pages={109445} }
 @article{amanullah_gao_osae_saroj_yang_liu_weng_luan_2021, title={Genetic linkage mapping and QTLs identification for morphology and fruit quality related traits of melon by SNP based CAPS markers}, volume={11}, url={https://doi.org/10.1016/j.scienta.2020.109849}, DOI={10.1016/j.scienta.2020.109849}, journal={Scientia Horticulturae}, publisher={Elsevier BV}, author={Amanullah, Sikandar and Gao, Peng and Osae, Benjamin Agyei and Saroj, Arvind and Yang, Tiantian and Liu, Shi and Weng, Yiqun and Luan, Feishi}, year={2021}, month={Feb}, pages={109849} }
 @article{qtl mapping for significant seed traits of watermelon (<i>citrullus lanatus</i> schrad.)_2021, url={https://publons.com/wos-op/publon/44106484/}, DOI={10.30848/PJB2021-3(24)}, journal={Pakistan Journal of Botany}, year={2021} }
 @article{qtl mapping for significant seed traits of watermelon (citrullus lanatus schrad)_2021, volume={53}, url={http://dx.doi.org/10.30848/pjb2021-3}, DOI={10.30848/pjb2021-3}, number={3}, journal={Pakistan Journal of Botany}, publisher={Pakistan Journal of Botany}, year={2021}, month={Jun} }
 @article{luan_fan_sun_cui_amanullah_tang_gao_2019, title={Genetic mapping reveals a candidate gene for egusi seed in watermelon}, volume={215}, url={http://dx.doi.org/10.1007/s10681-019-2504-4}, DOI={10.1007/s10681-019-2504-4}, number={11}, journal={Euphytica}, publisher={Springer Science and Business Media LLC}, author={Luan, Feishi and Fan, Chao and Sun, Lei and Cui, Haonan and Amanullah, Sikandar and Tang, Lili and Gao, Peng}, year={2019}, month={Nov} }
 @article{amanullah_liu_gao_zhu_zhu_fan_luan_2018, title={QTL mapping for melon ( Cucumis melo L.) fruit traits by assembling and utilization of novel SNPs based CAPS markers}, volume={236}, url={http://dx.doi.org/10.1016/j.scienta.2018.02.041}, DOI={10.1016/j.scienta.2018.02.041}, journal={Scientia Horticulturae}, publisher={Elsevier BV}, author={Amanullah, Sikandar and Liu, Shi and Gao, Peng and Zhu, Zicheng and Zhu, Qianglong and Fan, Chao and Luan, Feishi}, year={2018}, month={Jun}, pages={18–29} }
 @article{aloe vera coating efficiency on shelf life of eggplants at differential storage temperatures_2017, journal={Journal of Northeast Agricultural University (English Edition)}, year={2017} }
 @article{zhu_gao_liu_zhu_amanullah_davis_luan_2017, title={Comparative transcriptome analysis of two contrasting watermelon genotypes during fruit development and ripening}, volume={18}, url={http://dx.doi.org/10.1186/s12864-016-3442-3}, DOI={10.1186/s12864-016-3442-3}, abstractNote={Watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] is an economically important crop with an attractive ripe fruit that has colorful flesh. Fruit ripening is a complex, genetically programmed process. In this study, a comparative transcriptome analysis was performed to identify the regulators and pathways that are involved in the fruit ripening of pale-yellow-flesh cultivated watermelon (COS) and red-flesh cultivated watermelon (LSW177). We first identified 797 novel genes to extend the available reference gene set. Second, 3958 genes in COS and 3503 genes in LSW177 showed at least two-fold variation in expression, and a large number of these differentially expressed genes (DEGs) during fruit ripening were related to carotenoid biosynthesis, plant hormone pathways, and sugar and cell wall metabolism. Third, we noted a correlation between ripening-associated transcripts and metabolites and the key function of these metabolic pathways during fruit ripening. The results revealed several ripening-associated actions and provide novel insights into the molecular mechanisms underlying the regulation of watermelon fruit ripening.}, number={1}, journal={BMC Genomics}, publisher={Springer Science and Business Media LLC}, author={Zhu, Qianglong and Gao, Peng and Liu, Shi and Zhu, Zicheng and Amanullah, Sikandar and Davis, Angela R. and Luan, Feishi}, year={2017}, month={Dec} }
 @article{nisa_mallano_yu_chen_duan_amanullah_kousar_baloch_sun_tabys_et al._2017, title={GsSNAP33 , a novel Glycine soja SNAP25-type protein gene: Improvement of plant salt and drought tolerances in transgenic Arabidopsis thaliana}, volume={119}, url={http://dx.doi.org/10.1016/j.plaphy.2017.07.029}, DOI={10.1016/j.plaphy.2017.07.029}, journal={Plant Physiology and Biochemistry}, publisher={Elsevier BV}, author={Nisa, Zaib-un and Mallano, Ali Inayat and Yu, Yang and Chen, Chao and Duan, Xiangbo and Amanullah, Sikandar and Kousar, Abida and Baloch, Abdul Wahid and Sun, Xiaoli and Tabys, Dina and et al.}, year={2017}, month={Oct}, pages={9–20} }
 @article{li_zhao_zhu_zhang_fan_amanullah_gao_luan_2017, title={Mapping of powdery mildew resistance genes in melon ( Cucumis melo L.) by bulked segregant analysis}, volume={220}, url={http://dx.doi.org/10.1016/j.scienta.2017.04.001}, DOI={10.1016/j.scienta.2017.04.001}, journal={Scientia Horticulturae}, publisher={Elsevier BV}, author={Li, Bing and Zhao, Yulong and Zhu, Qianglong and Zhang, Zhipeng and Fan, Chao and Amanullah, Sikandar and Gao, Peng and Luan, Feishi}, year={2017}, month={Jun}, pages={160–167} }
 @article{postharvest treatment of salicylic acid on guava to enhance the shelf life at ambient temperature_2017, volume={10}, url={http://dx.doi.org/10.12692/ijb/10.3.92-106}, DOI={10.12692/ijb/10.3.92-106}, number={3}, journal={International Journal of Biosciences (IJB)}, publisher={International Journal of Biosciences}, year={2017}, month={Mar}, pages={92–106} }
 @article{abbas_ke_yu_yue_amanullah_jahangir_fan_2017, title={Volatile terpenoids: multiple functions, biosynthesis, modulation and manipulation by genetic engineering}, volume={246}, url={http://dx.doi.org/10.1007/s00425-017-2749-x}, DOI={10.1007/s00425-017-2749-x}, number={5}, journal={Planta}, publisher={Springer Science and Business Media LLC}, author={Abbas, Farhat and Ke, Yanguo and Yu, Rangcai and Yue, Yuechong and Amanullah, Sikandar and Jahangir, Muhammad Muzammil and Fan, Yanping}, year={2017}, month={Nov}, pages={803–816} }
 @article{assessment of nacl stress on morphological behavior of gazania_2016, journal={International Journal of Agronomy and Agricultural Research (IJAAR)}, year={2016} }
 @article{zhu_gao_liu_amanullah_luan_2016, title={Comparative analysis of single nucleotide polymorphisms in the nuclear, chloroplast, and mitochondrial genomes in identification of phylogenetic association among seven melon (<i>Cucumis melo</i> L.) cultivars}, volume={66}, url={http://dx.doi.org/10.1270/jsbbs.16066}, DOI={10.1270/jsbbs.16066}, abstractNote={A variety of melons are cultivated worldwide, and their specific biological properties make them an attractive model for molecular studies. This study aimed to investigate the single nucleotide polymorphisms (SNPs) from the mitochondrial, chloroplast, and nuclear genomes of seven melon accessions (Cucumis melo L.) to identify the phylogenetic relationships among melon cultivars with the Illumina HiSeq 2000 platform and bioinformatical analyses. The data showed that there were a total of 658 mitochondrial SNPs (207–295 in each), while there were 0–60 chloroplast SNPs among these seven melon cultivars, compared to the reference genome. Bioinformatical analysis showed that the mitochondrial tree topology was unable to separate the melon features, whereas the maximum parsimony/neighbor joining (MP/NJ) tree of the chloroplast SNPs could define melon features such as seed length, width, thickness, 100-seed weight, and type. SNPs of the nuclear genome were better than the mitochondrial and chloroplast SNPs in the identification of melon features. The data demonstrated the usefulness of mitochondrial, chloroplast, and nuclear SNPs in identification of phylogenetic associations among these seven melon cultivars.}, number={5}, journal={Breeding Science}, publisher={Japanese Society of Breeding}, author={Zhu, Qianglong and Gao, Peng and Liu, Shi and Amanullah, Sikandar and Luan, Feishi}, year={2016}, pages={711–719} }
 @article{effeciency of boron and magnesium levels on productivity of cauliflower (brassica oleracea l.)_2016, journal={International Journal of Advanced Research (IJAR)}, year={2016} }
 @article{exogenic function of salicylic acid to promote saline tolerance in cucumber_2016, journal={International Journal of Agricultural Sciences and Veterniray Medicine (IJASVM)}, year={2016} }
 @article{effectiveness of various salinity on leaf growth of gazania_2016, journal={International Journal of Agronomy and Agricultural Research (IJAAR)}, year={2016} }
 @article{germination response of weeds to different sources of phosphorus_2015, journal={Climate Change Outlook and Adaptation}, year={2015} }