Works (56)

Updated: May 20th, 2024 08:03

2024 journal article

A TriAdj-Adjuvanted <i>Chlamydia trachomatis</i> CPAF Protein Vaccine Is Highly Immunogenic in Pigs

VACCINES, 12(4).

By: J. Proctor n, M. Stadler*, L. Cortes n, D. Brodsky n, L. Poisson n, V. Gerdts*, A. Smirnov n, T. Smirnova n ...

author keywords: Chlamydia trachomatis; vaccination; adjuvant; chlamydial-protease-like activity factor; animal model; swine; one health
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: April 22, 2024

2024 article

Measuring local pH at interfaces from molecular tumbling: A concept for designing EPR-active pH-sensitive labels and probes

Voinov, M. A., Nunn, N., Rana, R., Davidsson, A., Smirnov, A. I., & Smirnova, T. I. (2024, April 18). ORGANIC & BIOMOLECULAR CHEMISTRY, Vol. 4.

By: M. Voinov n, N. Nunn n, R. Rana n, A. Davidsson n, A. Smirnov n & T. Smirnova n

Sources: ORCID, Web Of Science, NC State University Libraries
Added: May 1, 2024

2023 article

Changes in metabolic landscapes shape divergent but distinct mutational signatures and cytotoxic consequences of redox stress

Degtyareva, N. P., Placentra, V. C., Gabel, S. A., Klimczak, L. J., Gordenin, D. A., Wagner, B. A., … Doetsch, P. W. (2023, April 20). NUCLEIC ACIDS RESEARCH, Vol. 4.

MeSH headings : Humans; Antioxidants; Hydrogen Peroxide / toxicity; Mutation; Oxidation-Reduction; Neoplasms / genetics; Oxidants
TL;DR: This study provides the framework for understanding multidimensional processes triggered by agents collectively known as oxidants, and detects increased mutational loads associated with potassium bromate-related mutational motifs in human tumors may be clinically relevant as a biomarker of this distinct type of redox stress. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: May 1, 2023

2021 article

EPR Oximetry with Nitroxides: Effects of Molecular Structure, pH, and Electrolyte Concentration

Voinov, M. A., Smirnova, T. I., & Smirnov, A. I. (2021, November 20). APPLIED MAGNETIC RESONANCE, Vol. 11.

By: M. Voinov n, T. Smirnova n & A. Smirnov n

UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: November 20, 2021

2021 journal article

Integrative structural dynamics probing of the conformational heterogeneity in synaptosomal-associated protein 25

CELL REPORTS PHYSICAL SCIENCE, 2(11).

By: N. Saikia*, I. Yanez-Orozco*, R. Qiu n, P. Hao n, S. Milikisiyants n, E. Ou n, G. Hamilton*, K. Weninger n ...

TL;DR: An integrative method is reported to probe the structural dynamics of SNAP-25 by combining replica-exchange discrete molecular dynamics simulations and label-based experiments at ensemble and single-molecule levels and it is expected that this integrative approach may help further the understanding of IDPs. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: March 14, 2022

2021 journal article

Measuring plasma-generated center dot OH and O atoms in liquid using EPR spectroscopy and the non-selectivity of the HTA assay

JOURNAL OF PHYSICS D-APPLIED PHYSICS, 54(14).

By: B. Myers n, P. Ranieri n, T. Smirnova n, P. Hewitt n, D. Peterson n, M. Quesada n, E. Lenker n, K. Stapelmann n

author keywords: atmospheric pressure plasma jet; atomic oxygen; hydroxyl radicals; hydrogen peroxide; reactive oxygen species; plasma-liquid chemistry; EPR spectroscopy
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: January 9, 2021

2020 journal article

Alternative Reactivity of Leucine 5-Hydroxylase Using an Olefin-Containing Substrate to Construct a Substituted Piperidine Ring

BIOCHEMISTRY, 59(21), 1961–1965.

Contributors: L. Cha n, S. Milikisiyants n, M. Davidson n, S. Xue*, T. Smirnova n, A. Smirnov n, Y. Guo*, W. Chang n

MeSH headings : Alkenes / chemistry; Alkenes / metabolism; Leucine / chemistry; Leucine / metabolism; Mixed Function Oxygenases / chemistry; Mixed Function Oxygenases / metabolism; Molecular Conformation; Nostoc / enzymology; Substrate Specificity
TL;DR: It is demonstrated that the activity of an Fe/2OG enzyme can be redirected when changing the targeted carbon hybridization from sp3 to sp2 and the resulting epoxide subsequently undergoes intramolecular cyclization to form the substituted piperidine, 2S,5S-hydroxypipecolic acid. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: June 22, 2020

2019 journal article

Dielectric and Electrostatic Properties of the Silica Nanoparticle-Water Interface by EPR of pH-Sensitive Spin Probes

JOURNAL OF PHYSICAL CHEMISTRY C, 123(49), 29972–29985.

By: V. Perelygin n, M. Voinov n, A. Marek n, E. Ou n, J. Krim n, D. Brenner n, T. Smirnova n, A. Smirnov n

Contributors: V. Perelygin n, M. Voinov n, A. Marek n, E. Ou n, J. Krim n, D. Brenner n, T. Smirnova n, A. Smirnov n

UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: January 6, 2020

2019 article

EPR studies of bionanomaterials

ELECTRON MAGNETIC RESONANCE - APPLICATIONS IN PHYSICAL SCIENCES AND BIOLOGY, Vol. 50, pp. 129–159.

By: T. Smirnova n & A. Smirnov n

TL;DR: This chapter discusses some recent applications of EPR in studies bionanomaterials with particular emphasis on the interfacial properties of the nanoobjects and their interactions with biomacromolecules. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: November 18, 2019

2019 journal article

Effect of Silica Support on Electrostatics of Lipid Interfaces in Nano-Bio Hybrid Systems

Biophysical Journal, 116(3), 81a.

By: E. Ou n, M. Voinov n, A. Irving n, A. Smirnov n & T. Smirnova n

Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2019 review

Spontaneous Switching among Conformational Ensembles in Intrinsically Disordered Proteins

[Review of ]. BIOMOLECULES, 9(3).

By: U. Choi*, H. Sanabria*, T. Smirnova n, M. Bowen* & K. Weninger n

author keywords: dynamic configuration; free energy landscape; intrinsically disordered protein; IDP
MeSH headings : Humans; Intrinsically Disordered Proteins / chemistry; Protein Conformation
TL;DR: A growing number of proteins that appear intrinsically disordered by biochemical and bioinformatic characterization but switch between restricted regions of conformational space are described. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: April 29, 2019

2018 journal article

Elucidating the Reaction Pathway of Decarboxylation-Assisted Olefination Catalyzed by a Mononuclear Non-Heme Iron Enzyme

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 140(45), 15190–15193.

By: C. Yu n, Y. Tang*, L. Cha n, S. Milikisiyants n, T. Smirnova n, A. Smirnov n, Y. Guo*, W. Chang n

Contributors: C. Yu n, Y. Tang*, L. Cha n, S. Milikisiyants n, T. Smirnova n, A. Smirnov n, Y. Guo*, W. Chang n

MeSH headings : Alkenes / chemistry; Alkenes / metabolism; Biocatalysis; Decarboxylation; Molecular Structure; Nonheme Iron Proteins / metabolism
TL;DR: Experimental observations imply that a pathway involving an initial C-H activation followed by a benzylic carbocation species or by electron transfer coupled β-scission is likely utilized to complete C═C bond formation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: December 10, 2018

2018 journal article

Silica-Supported Lipid Bilayers: Electrostatic Effects at Lipid Interfaces as Reported by Spin-Labeling EPR

Biophysical Journal, 114(3), 96a.

By: E. Ou n, M. Voinov n, A. Smirnov n & T. Smirnova n

Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2018 journal article

Using Hyscore Spectroscopy of Nitroxides to Profile Water Content of Lipid Bilayers with 2 Å Spatial Resolution

Biophysical Journal, 114(3), 16a.

By: M. Chestnut n, S. Milikisiyants n, A. Koolivand n, M. Voynov n, T. Smirnova n & A. Smirnov n

UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020

2017 journal article

Effects of Silica Support on Dynamics of Transmembrane Peptides and Effective p K a of Ionisable Sidechains

Biophysical Journal, 112(3), 175a.

By: E. Ou n, M. Voinov n, A. Smirnov n & T. Smirnova n

Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2017 journal article

Glycol Chitosan Engineered Autoregenerative Antioxidant Significantly Attenuates Pathological Damages in Models of Age-Related Macular Degeneration

ACS Nano, 11(5), 4669–4685.

By: R. Mitra, R. Gao*, M. Zheng, M. Wu, M. Voinov n, A. Smirnov n, T. Smirnova n, K. Wang, S. Chavala*, Z. Han

Contributors: R. Mitra, R. Gao*, M. Zheng, M. Wu, M. Voinov n, A. Smirnov n, T. Smirnova n, K. Wang, S. Chavala*, Z. Han

author keywords: age-related macular degeneration; cerium oxide nanoparticles; antioxidant; reactive oxygen species; laser-induced choroidal neovascularization
MeSH headings : Animals; Antioxidants / pharmacology; Cerium / chemistry; Cerium / pharmacology; Choroidal Neovascularization / drug therapy; Choroidal Neovascularization / prevention & control; Disease Models, Animal; Human Umbilical Vein Endothelial Cells; Humans; Macular Degeneration / physiopathology; Macular Degeneration / therapy; Male; Mice; Mice, Inbred C57BL; Oxidative Stress / drug effects; Reactive Oxygen Species / metabolism; Reactive Oxygen Species / pharmacology; Vascular Endothelial Growth Factor A / metabolism
TL;DR: An engineered water-soluble, biocompatible, trackable nanoceria with enriched antioxidant activity to scavenge intracellular reactive oxygen species (ROS) is reported, which may offer a superior therapeutic effect to deal with oxidative stress induced pathogeneses, such as AMD. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2017 journal article

Oligomeric Structure of Anabaena Sensory Rhodopsin in a Lipid Bilayer Environment by Combining Solid-State NMR and Long-range DEER Constraints

JOURNAL OF MOLECULAR BIOLOGY, 429(12), 1903–1920.

By: S. Milikisiyants n, S. Wang*, R. Munro*, M. Donohue n, M. Ward*, D. Bolton*, L. Brown*, T. Smirnova n, V. Ladizhansky*, A. Smirnov n

Contributors: S. Milikisiyants n, S. Wang*, R. Munro*, M. Donohue n, M. Ward*, D. Bolton*, L. Brown*, T. Smirnova n, V. Ladizhansky*, A. Smirnov n

author keywords: double electron electron resonance (DEER); site-directed spin labeling; solid-state NMR; paramagnetic relaxation enhancement; membrane protein structure
MeSH headings : Anabaena / chemistry; Lipid Bilayers / chemistry; Magnetic Resonance Spectroscopy; Membrane Proteins / chemistry; Membrane Proteins / metabolism; Models, Biological; Models, Molecular; Protein Multimerization; Sensory Rhodopsins / chemistry; Sensory Rhodopsins / metabolism
TL;DR: The combined approach of using complementary DEER and NMR measurements for the determination of oligomeric structures would be widely applicable to membrane proteins where paramagnetic tags can be introduced. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2017 journal article

Oxidation of pyrrole by dehaloperoxidase-hemoglobin: chemoenzymatic synthesis of pyrrolin-2-ones

CATALYSIS SCIENCE & TECHNOLOGY, 7(14), 3104–3118.

By: N. McCombs n, T. Smirnova n & R. Ghiladi n

TL;DR: The results demonstrate the breadth of chemical reactivity afforded by dehaloperoxidase, and provide further evidence for establishing DHP as a multifunctional globin with practical applications as a biocatalyst. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2016 journal article

Synthesis of New Mixed-Metal Ammonium Vanadates: Cation Order versus Disorder, and Optical and Photocatalytic Properties

CRYSTAL GROWTH & DESIGN, 16(10), 5762–5770.

By: L. Luo n, E. Ou n, T. Smirnova n & P. Maggard n

Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2016 journal article

The Hydroxyl Radical is a Critical Intermediate in the Voltammetric Detection of Hydrogen Peroxide

Journal of the American Chemical Society, 138(8), 2516–2519.

By: J. Roberts n, M. Voinov n, A. Schmidt n, T. Smirnova n & L. Sombers n

Contributors: J. Roberts n, M. Voinov n, A. Schmidt n, T. Smirnova n & L. Sombers n

MeSH headings : Electrochemical Techniques / methods; Electron Spin Resonance Spectroscopy / methods; Hydrogen Peroxide / analysis; Hydrogen Peroxide / chemistry; Hydroxyl Radical / analysis; Hydroxyl Radical / chemistry; Pyrroles / chemistry; Spin Labels
TL;DR: Voltammetry was combined with electron paramagnetic resonance spectroscopy to identify and define the role of an oxygen-centered radical liberated during the oxidation of H2O2, and it was demonstrated that the hydroxyl radical is a principal contributor to the voltammetry of H1O2 as signal is attenuated when this species is trapped. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref, ORCID
Added: August 6, 2018

2016 journal article

smFRET and DEER Distance Measurements as Applied to Disordered and Structured Proteins

Biophysical Journal, 110(3), 559a.

By: K. Weninger n, R. Qiu n, E. Ou n, S. Milikisiyants n, H. Sanabria* & T. Smirnova n

TL;DR: Single molecule (sm) FRET reports on conformation of individual protein molecules whereas DEER provides information on ensemble average while the distance ranges of these two methods overlaps, and direct comparison of FRET and DEER data is rarely found in the literature. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 5, 2020

2015 journal article

Determining Oligomeric Order of a Membrane Protein by Double Electron-Electron Resonance Spectroscopy

Biophysical Journal, 108(2), 93a.

By: S. Milikisiyants n, S. Wang*, R. Munro*, M. Donohue n, L. Brown*, T. Smirnova n, V. Ladizhansky*, A. Smirnov n

TL;DR: The use of spin-labeling and Double Electron-Electron Resonance (DEER) spectroscopy to determine the oligomeric order of a membrane protein and the results demonstrate that DEER restraints can not only differentiate between the dimer and trimer models that have very different interfaces, but further rule out hypothetical tetramer and other higher order polygon models. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: February 24, 2020

2015 journal article

Ferromagnetic coupling in d(1)-d(3) linear oxido-bridged heterometallic complexes: ground-state models of metal-to-metal charge transfer excited states

DALTON TRANSACTIONS, 44(43), 18937–18944.

By: T. Huang n, X. Wu n, X. Song n, H. Xu n, T. Smirnova n, W. Weare n, R. Sommer n

TL;DR: A model that links either ferromagnetic or antiferromagentic exchange coupling with long excited state lifetimes in metal-to-metal charge transfer (MMCT) chromophores is proposed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2015 journal article

Manganese-Vanadate Hybrids: Impact of Organic Ligands on Their Structures, Thermal Stabilities, Optical Properties, and Photocatalytic Activities

INORGANIC CHEMISTRY, 54(15), 7388–7401.

By: L. Luo n, Y. Zeng n, L. Li n, Z. Luo*, T. Smirnova n & P. Maggard n

TL;DR: Manganese(II)-vanadate(V)/organic hybrids were prepared in high purity using four different N-donor organic ligands and their crystalline structures, thermal stabilities, optical properties, photocatalytic activities and electronic structures were investigated as a function of the organic ligand. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2015 journal article

“Snorkeling” of the Charged Sidechain of a Transmembrane Peptide as Directly Observed by Double Electron-Electron Resonance Experiment

Biophysical Journal, 108(2), 203a.

By: M. Donohue n, M. Voynov n, S. Milikisiyants n, A. Smirnov n & T. Smirnova n

TL;DR: Experimental observations of “snorkeling” for nitroxide-modified side-chains upon protonation, its dependence upon the location along the transmembrane peptide helix, and how this snorkeling is affected by the membrane electrostatic surface potential are reported. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Crossref, NC State University Libraries, ORCID
Added: August 28, 2020

2014 journal article

Copper-Organic/Octamolybdates: Structures, Bandgap Sizes, and Photocatalytic Activities

Inorganic Chemistry, 53(7), 3464–3470.

By: L. Luo n, H. Lin n, L. Li n, T. Smirnova n & P. Maggard n

TL;DR: The structures, optical bandgap sizes, and photocatalytic activities are described for three copper-octamolybdate hybrid solids prepared using hydrothermal methods, and the dissolution of the clusters in III is found to occur with the reduction of Cu(II) to Cu(I), followed by subsequent detachment from the octamoly bdate cluster. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries, Web Of Science
Added: August 6, 2018

2014 journal article

Identification of free radicals in pyrolysis oil and their impact on bio-oil stability

RSC ADVANCES, 4(56), 29840–29846.

By: J. Meng n, T. Smirnova n, X. Song n, A. Moore n, X. Ren n, S. Kelley n, S. Park n, D. Tilotta n

UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Profiling the Dielectric Constant at the Membrane-Peptide Interface using Ionizable EPR Probes

Biophysical Journal, 106(2), 508a.

By: M. Donohue n, M. Voynov n, A. Smirnov n & T. Smirnova n

TL;DR: This work reports on employing pH-sensitive ionizable EPR labels to profile a heterogeneous dielectric environment along the α-helix of a WALP peptide integrated in a lipid bilayer, and for the first time measurements of local electrostatics at peptide-bilayer interface were based on direct distance measurements rather than on assumptions on the probe location. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: February 24, 2020

2014 journal article

Structure, Dynamics, and Electrostatic Effects on Membrane Binding of Nod Peptides

Biophysical Journal, 106(2), 295a.

By: M. Quinones n, V. Bankaitis* & T. Smirnova n

TL;DR: Three peptides mimicking the sequence thought to be responsible for the targeted membrane binding containing single Cys substitutions are synthesized and substantial differences in binding behavior of these charged peptides were observed despite the similarity in sequences. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2013 journal article

Isoprenoid Biosynthesis: Ferraoxetane or Allyl Anion Mechanism for IspH Catalysis?

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 52(25), 6522–6525.

author keywords: biosynthesis; enzyme catalysis; iron-sulfur cluster; metalloproteins; terpenes
MeSH headings : Anions; Catalysis; Ferric Compounds / metabolism; Models, Molecular; Proteins / metabolism; Terpenes / metabolism
TL;DR: In plant plastids, IPP and DMAPP are produced primarily via the 2-C-methylerythritol 4-phosphate (MEP) pathway from E-1-hydroxy- 2-methyl-but-2-enyl 4-diph phosphate (HMBPP) in a reaction catalyzed by the enzyme IspH, and this route is also used in most bacteria, as well as in malaria parasites. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

Molecular pH Probes at a Protein-Lipid Interface: Assessment of Local Dielectric Environment for Transmembrane Peptide

Biophysical Journal, 104(2), 373a.

By: M. Donohue n, M. Voynov n, A. Smirnov n & T. Smirnova n

TL;DR: Measurements of local electrostatics at peptide-bilayer interface were based on direct distance measurements rather than on assumptions on the probe location, allowing us to estimate effective dielectric constant. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: February 24, 2020

2013 journal article

The UDP-diacylglucosamine Pyrophosphohydrolase LpxH in Lipid A Biosynthesis Utilizes Mn2+ Cluster for Catalysis

JOURNAL OF BIOLOGICAL CHEMISTRY, 288(38), 26987–27001.

By: H. Young*, M. Donohue n, T. Smirnova n, A. Smirnov n & P. Zhou*

Contributors: H. Young*, M. Donohue n, T. Smirnova n, A. Smirnov n & P. Zhou*

MeSH headings : Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / isolation & purification; Bacterial Proteins / metabolism; Catalysis; Cloning, Molecular; Gene Expression; Haemophilus influenzae / enzymology; Haemophilus influenzae / genetics; Lipid A / biosynthesis; Lipid A / chemistry; Lipid A / metabolism; Manganese / chemistry; Manganese / metabolism; Pyrophosphatases / chemistry; Pyrophosphatases / genetics; Pyrophosphatases / isolation & purification; Pyrophosphatases / metabolism
TL;DR: Overall, this work unambiguously establishes LpxH as a calcineurin-like phosphoesterase containing a Mn2+ cluster coordinated by conserved residues, which sets the scene for further structural investigation of the enzyme and for design of novel antibiotics targeting lipid A biosynthesis. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2013 journal article

Tyrosyl Radicals in Dehaloperoxidase

Journal of Biological Chemistry, 288(46), 33470–33482.

By: R. Dumarieh n, J. D'Antonio n, A. Deliz-Liang n, T. Smirnova n, D. Svistunenko* & R. Ghiladi n

Contributors: R. Dumarieh n, . J. D'Antonio, A. Deliz-Liang n, T. Smirnova n, D. Svistunenko* & R. Ghiladi n

author keywords: Hemoglobin; Iron; Peroxidase; Radicals; Tyrosine; Compound ES; Compound I; Compound II; Dehaloperoxidase; Ferryl
MeSH headings : Adaptation, Physiological; Animals; Hemoglobins / chemistry; Hemoglobins / genetics; Hemoglobins / metabolism; Oxidation-Reduction; Oxygen / chemistry; Oxygen / metabolism; Peroxidase / chemistry; Peroxidase / genetics; Peroxidase / metabolism; Peroxidases / chemistry; Peroxidases / genetics; Peroxidases / metabolism; Polychaeta / enzymology; Protein Binding; Tyrosine / analogs & derivatives; Tyrosine / chemistry; Tyrosine / genetics; Tyrosine / metabolism
TL;DR: The results indicate that radicals form on all three tyrosines in DHP, likely an evolutionary adaptation allowing DHP to avoid self-inflicted damage in the oxidative environment. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: February 24, 2020

2013 journal article

Tyrosyl radicals in dehaloperoxidase how nature deals with evolving an oxygen-binding globin to a biologically relevant peroxidase

Journal of Biological Chemistry, 288(46), 33470–33482.

By: R. Dumarieh, J. D'Antonio, A. Deliz-Liang, T. Smirnova, D. Svistunenko & R. Ghiladi

Source: NC State University Libraries
Added: August 6, 2018

2012 journal article

Probing Dielectric and Hydrogen Bonding Gradients in Biological Membranes

Biophysical Journal, 102(3), 414a.

By: T. Smirnova n, M. Voynov n, O. Poluektov* & A. Smirnov n

TL;DR: An arsenal of advanced spin-labeling EPR methods are employed to profile heterogeneous dielectric and hydrogen bonding environment along the α-helical chain of an alanine-rich WALP peptide that is anchored in a lipid bilayer in a transmembrane orientation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries, ORCID
Added: February 24, 2020

2011 journal article

An ENDOR and HYSCORE Investigation of a Reaction Intermediate in IspG (GcpE) Catalysis

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 133(22), 8400–8403.

MeSH headings : Alkyl and Aryl Transferases / biosynthesis; Alkyl and Aryl Transferases / chemistry; Catalysis; Electron Spin Resonance Spectroscopy; Enzymes / chemistry; Models, Molecular; Molecular Structure
TL;DR: Using electron-nuclear double resonance and hyperfine sublevel correlation spectroscopies on labeled samples, results help clarify the nature of the reaction intermediate, supporting a direct interaction between the unique fourth Fe in the cluster and C2 and O3 of the ligand. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Pyridine Inhibitor Binding to the 4Fe-4S Protein A. aeolicus IspH (LytB): A HYSCORE Investigation

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 133(17), 6525–6528.

By: W. Wang, J. Li, K. Wang*, T. Smirnova n & E. Oldfield

MeSH headings : Anti-Bacterial Agents / chemistry; Anti-Bacterial Agents / pharmacology; Bacteria / drug effects; Bacteria / metabolism; Bacterial Proteins / metabolism; Drug Design; Iron-Sulfur Proteins / metabolism; Models, Molecular; Protein Binding; Pyridines / chemistry; Pyridines / pharmacology; Terpenes / metabolism
TL;DR: It is shown that pyridine inhibitors of IspH directly bind to the unique fourth Fe in the 4Fe-4S cluster, opening up new routes to inhibitor design, of interest in the context of both anti-bacterial as well as anti-malarial drug discovery. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Resurrection of a functional phosphatidylinositol transfer protein from a pseudo-Sec14 scaffold by directed evolution

MOLECULAR BIOLOGY OF THE CELL, 22(6), 892–905.

By: G. Schaaf*, M. Dynowski*, C. Mousley*, S. Shah*, P. Yuan*, E. Winklbauer*, M. Campos*, K. Trettin* ...

MeSH headings : Amino Acid Sequence; Cell Cycle Proteins / chemistry; Cell Cycle Proteins / genetics; Cell Cycle Proteins / metabolism; Chromosomal Proteins, Non-Histone / chemistry; Chromosomal Proteins, Non-Histone / genetics; Chromosomal Proteins, Non-Histone / metabolism; Directed Molecular Evolution; Endosomes / metabolism; Golgi Apparatus / metabolism; Models, Molecular; Molecular Dynamics Simulation; Molecular Sequence Data; Phenotype; Phosphatidylcholines / chemistry; Phosphatidylcholines / metabolism; Phosphatidylinositols / chemistry; Phosphatidylinositols / metabolism; Phospholipid Transfer Proteins / chemistry; Phospholipid Transfer Proteins / genetics; Phospholipid Transfer Proteins / metabolism; Protein Conformation; Saccharomyces cerevisiae / cytology; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism; Saccharomyces cerevisiae Proteins / chemistry; Saccharomyces cerevisiae Proteins / genetics; Saccharomyces cerevisiae Proteins / metabolism; Sequence Alignment; Signal Transduction; trans-Golgi Network / metabolism
TL;DR: A directed evolution approach is used as a novel prism through which the functional engineering of a Sec14-like phosphatidylinositol transfer protein can be observed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Role of Electrostatic and Hydrogen Bonding Environment in Sequestering Lipids from Membranes Into the Sec14 Protein Cavity

Biophysical Journal, 100(3), 552a–553a.

By: T. Smirnova*, T. Chadwick, V. Bankaitis, G. Schaaf, O. Poluektov & A. Smirnov

TL;DR: An arsenal of advanced spin-labeling EPR methods are employed to probe local electrostatic and hydrogen bonding environment that govern binding of lipids by Sec14 protein and indicate that the phospholipid-binding cavity of Sec14p with the likely sequestered water molecules provides a close match for the polarity profile along the bound PtdCho molecule and the one determined for membrane bilayers. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Crossref, NC State University Libraries, ORCID
Added: February 24, 2020

2011 journal article

Surface-Mediated Production of Hydroxyl Radicals as a Mechanism of Iron Oxide Nanoparticle Biotoxicity

Journal of the American Chemical Society, 133(1), 35–41.

By: M. Voinov n, J. Pagán n, E. Morrison n, T. Smirnova n & A. Smirnov n

Contributors: M. Voinov n, J. Pagán n, E. Morrison n, T. Smirnova n & A. Smirnov n

MeSH headings : Animals; Catalysis; Cattle; Cell Survival / drug effects; Electron Spin Resonance Spectroscopy; Ferric Compounds / chemistry; Ferric Compounds / toxicity; HeLa Cells; Humans; Hydrogen Peroxide / chemistry; Hydroxyl Radical / chemistry; Iron / chemistry; Nanoparticles / chemistry; Nanoparticles / toxicity; Oleic Acid / chemistry; Serum Albumin, Bovine / chemistry; Surface Properties
TL;DR: The experimental protocols developed in this study could be used as one of the approaches for developing analytical assays for assessing the free radical generating activity of a variety of nanomaterials that is potentially related to their biotoxicity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2010 journal article

Heterogeneous Dielectric and Hydrogen Bonding Environment of Transmembrane Peptides

Biophysical Journal, 98(3), 87a.

By: T. Smirnova n, M. Voynov n, O. Poluektov* & A. Smirnov n

TL;DR: It was concluded that such combination of EPR techniques significantly expands the capabilities of spin-labeling methods in studies of membrane proteins as demonstrated by deriving profiles of heterogeneous dielectric and hydrogen bonding environment along a typical transmembrane alpha-helix. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries, ORCID
Added: February 24, 2020

2010 journal article

Spectroscopic and Mechanistic Investigations of Dehaloperoxidase B fromAmphitrite ornata

Biochemistry, 49(31), 6600–6616.

By: J. D’Antonio n, E. D’Antonio n, M. Thompson n, E. Bowden n, S. Franzen n, T. Smirnova n, R. Ghiladi n

Contributors: . J. D'Antonio, . E.L. D'Antonio n, M. Thompson n, E. Bowden n, S. Franzen n, T. Smirnova n, R. Ghiladi n

MeSH headings : Animals; Cloning, Molecular; Halogenation; Hemoglobins; Iron; Kinetics; Oxidation-Reduction; Oxygen / metabolism; Peroxidases / genetics; Peroxidases / metabolism; Polychaeta / enzymology; Spectrum Analysis
TL;DR: A novel reaction pathway is demonstrated in which the products of the oxidative dehalogenation of trihalophenols (dihaloquinones) are themselves capable of inducing formation of oxyferrous DHP B, and an updated catalytic cycle for DHP is proposed. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2009 journal article

Characterization of Dehaloperoxidase Compound ES and Its Reactivity with Trihalophenols†

Biochemistry, 48(5), 995–1005.

By: J. Feducia n, R. Dumarieh n, L. Gilvey n, T. Smirnova n, S. Franzen n & R. Ghiladi n

Contributors: J. Feducia n, R. Dumarieh n, L. Gilvey n, T. Smirnova n, S. Franzen n & R. Ghiladi n

MeSH headings : Animals; Binding Sites; Chlorophenols / chemistry; Chlorophenols / metabolism; Crystallography, X-Ray; Cytochrome-c Peroxidase / chemistry; Cytochrome-c Peroxidase / metabolism; Electron Transport Complex IV / chemistry; Electron Transport Complex IV / metabolism; Hemoglobins / chemistry; Hemoglobins / metabolism; Peroxidases / chemistry; Peroxidases / metabolism; Polychaeta / enzymology
TL;DR: It is demonstrated that the first detectable intermediate following the addition of hydrogen peroxide to ferric DHP contains both a ferryl heme and a tyrosyl radical, analogous to Compound ES of cytochrome c peroxidase. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 6, 2018

2009 journal article

Membrane insertion of peptides mimicking E2 domain of Sindbis virus is modulated by cholesterol

Biophysical Journal, 96(3), 389a–390a.

By: T. Chadwick n, G. Rabah n, B. Davies n & T. Smirnova n

TL;DR: Investigation of the interaction of synthetic peptides mimicking E2 domain mutants with lipid bilayers with the goal to understand constraints placed upon membrane spanning domains for correct integration into the bilayer shows that cholesterol content of the lipid mixture modulates insertion of the peptides into bilayer mimicking mammalian cell membrane. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Crossref, NC State University Libraries, ORCID
Added: February 24, 2020

2009 journal article

Spectroscopic Probes of the Reactive Intermediates of Dehaloperoxidase from Amphitrite ornata

Biophysical Journal, 96(3), 437a.

By: R. Ghiladi n, R. Dumarieh n, M. Thompson n, Z. Wang n, T. Smirnova n & S. Franzen n

TL;DR: While unreactive toward further oxidation, Compound RH can be reduced and subsequently bind dioxygen, generating oxyferrous DHP, which may represent the catalytic link between the peroxidase and oxygen-transport activities in this bifunctional protein. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2009 journal article

Substrate binding triggers a switch in the iron coordination in dehaloperoxidase from Amphitrite Ornate

Biophysical Journal, 96(3), 437a.

By: T. Smirnova n, M. Davis n, R. Weber & S. Franzen n

TL;DR: Hyperfine sublevel correlation spectroscopic analysis of the ferric form of DHP was carried out to characterize effects of the substrate 2,4,6-trifluorophenol (TFP) binding on the iron coordination in order to elucidate molecular mechanisms responsible for switching the protein function from a globin to a peroxidase. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020

2008 journal article

Characterization of magnetic and electronic properties of trimetallic nitride endohedral fullerenes by SQUID magnetometry and electron paramagnetic resonance

CHEMICAL PHYSICS LETTERS, 453(4-6), 233–237.

By: T. Smirnova n, A. Smirnov n, T. Chadwick n & K. Walker*

Contributors: T. Smirnova n, A. Smirnov n, T. Chadwick n & K. Walker*

UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2008 journal article

Substrate binding triggers a switch in the iron coordination in dehaloperoxidase from Amphitrite ornata: HYSCORE experiments

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 130(7), 2128-+.

By: T. Smirnova n, R. Weber n, M. Davis n & S. Franzen n

MeSH headings : Animals; Electron Spin Resonance Spectroscopy; Ferric Compounds / chemistry; Ferric Compounds / metabolism; Heme / chemistry; Heme / metabolism; Hemoglobins / chemistry; Hemoglobins / metabolism; Iron / chemistry; Iron / metabolism; Models, Molecular; Peroxidases / chemistry; Peroxidases / metabolism; Polychaeta / enzymology; Protein Binding; Spectrum Analysis / methods; Water / chemistry; Water / metabolism
TL;DR: Continuous wave EPR spectra show that heme iron of DHP at pH 6.0 exists in the high spin state, consistent with a water molecule being the sixth ligand in the iron coordination, and highlights the proposed role of the substrate as a trigger for the switch from hemoglobin to peroxidase. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Binding of MRI contrast agents to albumin: a high-field EPR study

APPLIED MAGNETIC RESONANCE, 31(3-4), 431–446.

By: T. Smirnova n

TL;DR: Analysis of the line width broadening effects for spectra from a series ofn-doxyl-stearic acids bound to HSA indicated a progressive decrease of spin label-Gd(III) magnetic interactions along the fatty acid (FA) binding channel, consistent with binding of Gd-DOTAP complex in the vicinity of the main FA binding site. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Geometry of hydrogen bonds formed by lipid bilayer nitroxide probes: A high-frequency pulsed ENDOR/EPR study

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 129(12), 3476-+.

By: T. Smirnova n, A. Smirnov n, S. Paschenko n & O. Poluektov n

Contributors: T. Smirnova n, A. Smirnov n, S. Paschenko n & O. Poluektov n

MeSH headings : Electron Spin Resonance Spectroscopy; Hydrogen Bonding; Lipid Bilayers / chemistry; Molecular Structure; Nitrogen Oxides / chemistry
TL;DR: The hypothesis that HF EPR spectra are exclusively sensitive to formation of hydrogen bonds and could be used for probing the hydrogen bond network in complex biomolecular assemblies and lipid bilayers with site-directed spin-labeling methods is strengthened. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2007 journal article

Local polarity and hydrogen bonding inside the Sec14p phospholipid-binding cavity: High-field multi-frequency electron paramagnetic resonance studies

BIOPHYSICAL JOURNAL, 92(10), 3686–3695.

By: T. Smirnova n, T. Chadwick n, M. Voinov n, O. Poluektov*, J. Tol*, A. Ozarowski*, G. Schaaf*, M. Ryan*, V. Bankaitis*

Contributors: T. Smirnova n, T. Chadwick n, M. Voinov n, O. Poluektov*, J. Van Tol*, A. Ozarowski*, G. Schaaf*, M. Ryan*, V. Bankaitis*

MeSH headings : Binding Sites; Computer Simulation; Electron Spin Resonance Spectroscopy / methods; Hydrogen Bonding; Models, Chemical; Models, Molecular; Phospholipid Transfer Proteins / chemistry; Phospholipids / chemistry; Protein Binding; Saccharomyces cerevisiae Proteins / chemistry; Solvents / chemistry; Static Electricity
TL;DR: The data suggest the polarity gradient inside the Sec14p cavity is a significant contributor to the driving molecular forces for extracting a phospholipid from the bilayer and the enhanced g-factor resolution of EPR provides researchers with a spectroscopic tool to deconvolute two major contributions to the x-component of the nitroxide g-matrix: hydrogen-bond formation and local electrostatic effects. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2006 journal article

Cryogen-free superconducting magnet system for multifrequency electron paramagnetic resonance up to 12.1T

Review of Scientific Instruments, 77(3), 035108.

By: A. Smirnov n, T. Smirnova n, R. MacArthur n, J. Good & R. Hall

Contributors: A. Smirnov n, T. Smirnova n, R. MacArthur n, J. Good & R. Hall

UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Crossref, NC State University Libraries, ORCID, NC State University Libraries
Added: August 6, 2018

2006 journal article

The chemistry of phospholipid binding by the Saccharomyces cerevisiae phosphatidylinositol transfer protein Sec14p as determined by EPR spectroscopy

JOURNAL OF BIOLOGICAL CHEMISTRY, 281(46), 34897–34908.

By: T. Smirnova n, T. Chadwick n, R. MacArthur n, O. Poluektov*, L. Song*, M. Ryan*, G. Schaaf*, V. Bankaitis*

MeSH headings : Binding Sites; Gene Expression Regulation; Models, Molecular; Molecular Structure; Phosphatidylcholines / chemistry; Phosphatidylcholines / metabolism; Phospholipid Transfer Proteins / chemistry; Phospholipid Transfer Proteins / metabolism; Phospholipids / chemistry; Phospholipids / metabolism; Protein Binding; Saccharomyces cerevisiae / metabolism; Saccharomyces cerevisiae Proteins / chemistry; Saccharomyces cerevisiae Proteins / metabolism; Spectrometry, Mass, Electrospray Ionization / methods
TL;DR: The application of EPR spectroscopy is described to probe the local dynamics and the electrostatic microenvironment of phosphatidylcholine bound by Sec14p in a soluble protein-PtdCho complex and suggests a headgroup-out orientation ofSec14p-bound PtdCho. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Dynamic molecular oxygen accessibility to a buried Mn2+ protein site: A high-field EPR experiment

JOURNAL OF PHYSICAL CHEMISTRY B, 107(29), 7212–7215.

By: T. Smirnova n & A. Smirnov n

UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2001 journal article

Resolving domains of interdigitated phospholipid membranes with 95 GHz spin labeling EPR

APPLIED MAGNETIC RESONANCE, 21(3-4), 453–467.

By: A. Smirnov n & T. Smirnova n

Contributors: A. Smirnov n & T. Smirnova n

UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

1998 journal article

Multi-frequency EPR determination of zero field splitting of high spin species in liquids: Gd(III) chelates in water

Molecular Physics, 95(6), 1325–1332.

By: R. Clarkson*, A. Smirnov*, T. Smirnova*, H. Kang*, R. Belford*, K. Earle*, J. Freed*

Contributors: R. Clarkson*, A. Smirnov*, T. Smirnova*, H. Kang*, R. Belford*, K. Earle*, J. Freed*

UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, NC State University Libraries, ORCID
Added: August 28, 2020

Employment

Updated: August 11th, 2021 19:08

North Carolina State University Raleigh, North Carolina, US
Associate Professor Chemistry

Education

Updated: September 30th, 2022 17:47

University of Illinois Urbana-Champaign Urbana, Illinois, US
PhD Chemistry

1997 - 2000

University of Illinois Urbana-Champaign Urbana, Illinois, US
Postdoctoral Fellow

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