@article{jang_zhao_kim_pasquetti_kim_2023, title={Effects of bacterial β-mannanase on apparent total tract digestibility of nutrients in various feedstuffs fed to growing pigs}, volume={36}, ISSN={["2765-0235"]}, url={https://doi.org/10.5713/ab.23.0158}, DOI={10.5713/ab.23.0158}, abstractNote={Objective: The objective of this study was to determine the effects of β-mannanase on metabolizable energy (ME) and apparent total tract digestibility (ATTD) of protein in various feedstuffs including barley, copra meal, corn, corn distillers dried grains with solubles (DDGS), palm kernel meal, sorghum, and soybean meal.Methods: A basal diet was formulated with 94.8% corn and 0.77% amino acids, minerals, and vitamins and test diets replacing corn-basal diets with barley, corn DDGS, sorghum, soybean meal, or wheat (50%, respectively) and copra meal or palm kernel meal (30%, respectively). The basal diet and test diets were evaluated by using triplicated or quadruplicated 2×2 Latin square designs consisting of 2 diets and 2 periods with a total of 54 barrows at 20.6±0.6 kg (9 wk of age). Dietary treatments were levels of β-mannanase supplementation (0 or 800 U/kg of feed). Fecal and urine samples were collected for 4 d following a 4-d adaptation period. The ME and ATTD of crude protein (CP) in feedstuffs were calculated by a difference procedure. Data were analyzed using Proc general linear model of SAS.Results: Supplementation of β-mannanase improved (p<0.05) ME of barley (10.4%), palm kernel meal (12.4%), sorghum (6.0%), and soybean meal (2.9%) fed to growing pigs. Supplementation of β-mannanase increased (p<0.05) ATTD of CP in palm kernel meal (8.8%) and tended to increase (p = 0.061) ATTD of CP in copra meal (18.0%) fed to growing pigs.Conclusion: This study indicates that various factors such as the structure and the amount of β-mannans, water binding capacity, and the level of resistant starch vary among feedstuffs and the efficacy of supplemental β-mannanase may be influenced by these factors.}, number={11}, journal={ANIMAL BIOSCIENCE}, author={Jang, Ki Beom and Zhao, Yan and Kim, Young Ihn and Pasquetti, Tiago and Kim, Sung Woo}, year={2023}, month={Nov}, pages={1700–1708} }
 @article{guo_pasquetti_kim_2019, title={Lowering dietary cation-anion difference increases sow blood and milk calcium concentrations}, volume={97}, ISSN={0021-8812 1525-3163}, url={http://dx.doi.org/10.1093/jas/skz146}, DOI={10.1093/jas/skz146}, abstractNote={AbstractA study was conducted to evaluate the effects of feeding an acidogenic diet with a low dietary cation-anion difference (DCAD) on acid-base balance, blood, milk, and urine Ca concentrations of sows during lactation. A total of 30 multiparous sows (parity: 4.5 ± 2.9, Smithfield Premium Genetic, Rose Hill, NC) were allotted to 1 of 2 dietary treatments: CON (control diets were corn-soybean meal based with a calculated DCAD of 170 and 226 mEq/kg during late gestation and lactation, respectively) or ACI (acidogenic diets had a DCAD 100 mEq/kg lower than the control diets). The lower DCAD was achieved by the addition of an acidogenic mineral. The DCAD was calculated as mEq (Na + K – Cl)/kg diet. Sows had a daily access to 2-kg feed from day 94 of gestation to parturition and ad libitum access to feed during lactation. Blood and urine pH and Ca, serum macrominerals, serum biochemistry, Ca-regulating hormones, and milk composition were measured. Sows in ACI had a lower (P < 0.05) blood pH than sows in CON at day 1 of lactation. Sows in ACI had a lower (P < 0.05) urine pH at day 108 of gestation, days 1, 9, and 18 of lactation compared with sows in CON. Sows in ACI had greater (P < 0.05) concentrations of serum total Ca at days 1 and 18 of lactation than sows in CON. There was a greater (P < 0.05) concentration of colostrum Ca in ACI than in CON. There was no difference in urine Ca concentration between treatments during lactation. Concentrations of parathyroid hormone and 1,25-dihydroxycholecalciferol were not different between treatments at either day 1 or 18 of lactation. Sows in ACI tended to have a smaller (P = 0.086) concentration of total alkaline phosphatase in serum at day 18 of lactation compared with sows in CON. At day 1 of lactation, the concentration of serum Cl in ACI was greater (P < 0.05) than that in CON. Feed intake, BW loss, and litter performance were not different between treatments. Collectively, feeding an acidogenic diet with a low DCAD to sows can induce a mild metabolic acidosis at farrowing, reduce the urine pH consistently, and increase serum total Ca and colostrum Ca concentrations during lactation but without altering the parathyroid hormone and 1,25-dihydroxycholecalciferol levels during lactation.}, number={7}, journal={Journal of Animal Science}, publisher={Oxford University Press (OUP)}, author={Guo, Ji Yao and Pasquetti, Tiago Junior and Kim, Sung Woo}, year={2019}, month={Apr}, pages={2927–2939} }
 @article{park_pasquetti_malheiros_ferket_kim_2018, title={Effects of supplemental L-methionine on growth performance and redox status of turkey poults compared with the use of DL-methionine}, volume={97}, ISSN={0032-5791}, url={http://dx.doi.org/10.3382/ps/pex259}, DOI={10.3382/ps/pex259}, abstractNote={ABSTRACT This study was conducted to test the effects of dietary supplementation of feed grade L‐Met on growth performance and redox status of turkey poults compared with the use of conventional DL‐Met. Three hundred and eighty five newly hatched turkey poults were weighed and allotted to 5 treatments in a completely randomized design and the birds were fed dietary treatments for 28 d, including a basal diet (BD), the BD + 0.17 or 0.33% DL‐Met or L‐Met (representing 60, 75, and 90% of the requirement by National Research Council (NRC) for S containing AA, respectively). Increasing Met supplementation from 0 to 0.33% increased (P < 0.05) weight gain (690 to 746 g) and feed intake (1,123 to 1,248 g) of turkey poults. Supplementing L‐Met tended (P = 0.053) to reduce feed to gain ratio (1.70 to 1.63) compared with DL‐Met. The relative bioavailability of L‐Met to DL‐Met was 160% based on a multilinear regression analysis of weight gain. Supplementing Met regardless of its sources decreased (P < 0.05) malondialdehyde (3.29 to 2.47 nmol/mg protein) in duodenal mucosa compared with birds in the BD. Supplementing L‐Met tended (P = 0.094) to decrease malondialdehyde (1.27 to 1.16 nmol/mg protein) and increase glutathione (3.21 to 3.45 nmol/mg protein) in the liver compared with DL‐Met. Total antioxidant capacity, protein carbonyl, and morphology of duodenum and jejunum were not affected by Met sources. In conclusion, dietary supplementation of 0.33% Met to a diet with S containing AA meeting 60% of the NRC requirement enhanced weight gain, feed intake, and redox status by reducing oxidative stress in the gut and liver of turkey poults during the first 28 d of age. Use of L‐Met tended to enhance feed efficiency and was more effective in reducing oxidative stress and increasing glutathione in the liver compared with the use of DL‐Met. The use of L‐Met as a source of Met replacing DL‐Met seems to be beneficial to turkey poults during the first 28 d of age.}, number={1}, journal={Poultry Science}, publisher={Elsevier BV}, author={Park, I. and Pasquetti, T. and Malheiros, R.D. and Ferket, P.R. and Kim, S.W.}, year={2018}, month={Jan}, pages={102–109} }