@article{gregory_livingston_hawkins_loyola_cave_vaden_deresienski_breen_riofrio-lazo_lewbart_et al._2023, title={Dirofilaria immitis Identified in Galapagos Sea Lions (Zalophus wollebaeki): A Wildlife Health and Conservation Concern}, volume={59}, ISSN={["1943-3700"]}, DOI={10.7589/JWD-D-22-00119}, abstractNote={Abstract: The Galapagos sea lion (Zalophus wollebaeki), an endemic and endangered pinniped, faces an increasing threat due to infectious diseases related to domestic animals. Dirofilaria immitis, the parasite responsible for canine heartworm disease, is one such threat, as canine infections on the archipelago have been documented. We used a canine heartworm antigen test kit to analyze the blood from 25 juvenile Galapagos sea lions for D. immitis. Two (8%) sea lions tested positive for D. immitis antigen. Using morphologic and genetic assessments, we evaluated 20 filarial-like worms collected from within the heart of an adult male Galapagos sea lion during a previous routine postmortem examination. The intracardiac worms were morphologically consistent with adult D. immitis, and sequence analysis of targeted PCR amplicons confirmed their identity. This is the first report of D. immitis infection in Galapagos sea lions, which could become a major health problem for these pinnipeds. Further studies are necessary to confirm the level of threat from this parasite; however, widespread adoption of routine heartworm testing, prevention, and treatment in the canine population, and the control of mosquitos, could potentially reduce the disease impact on this endangered pinniped species.}, number={3}, journal={JOURNAL OF WILDLIFE DISEASES}, author={Gregory, Taylor M. and Livingston, Isabella and Hawkins, Eleanor C. and Loyola, Andrea and Cave, Ashley and Vaden, Shelly L. and Deresienski, Diane and Breen, Matthew and Riofrio-Lazo, Marjorie and Lewbart, Gregory A. and et al.}, year={2023}, month={Jul}, pages={487–494} }
 @article{gregory_heniff_gorges_lathan_lewbart_balko_2022, title={Development and Evaluation of a Serum Transfusion Process in the Thorny Devil Stick Insect (Eurycantha calcarata)}, volume={9}, ISSN={["2297-1769"]}, DOI={10.3389/fvets.2022.847043}, abstractNote={The thorny devil stick insect (Eurycantha calcarata) is a common invertebrate maintained under human care. Blood (hemolymph) transfusions are a widely used therapeutic tool in other species, but investigation in terrestrial arthropods remains scarce. Study objectives were development and evaluation of a serum transfusion process in the thorny devil stick insect. Twenty-five clinically healthy adult insects (9.9–23.0 g) were enrolled and baseline body weights were collected. Hemolymph collection was not successful in males, thus, all were recipient only (n = 12, MR). Females were divided into donor (n = 4, FD) or donor and recipient (n = 9, FDR) groups. Females were manually restrained and up to 1 mL of hemolymph was collected from the abdominal segment caudal to the proximal hindlimb using an 18 g hypodermic needle and passive collection via gravity. Hemolymph was quantified, centrifuged, and the serum separated. Insects were then injected superficially at the lateral aspect of the abdomen with 0.01 mL/g FD serum (MR), lactated Ringer's solution (LRS) equal to collected hemolymph volume (FD), or a combination of FDR serum and LRS equal to collected hemolymph volume (FDR). Response to stimulation, surface temperature, and righting reflex and mortality were serially assessed for up to 24 h and 7 days, respectively, following injection. In FD, median (range) injected LRS dose was 0.04 (0.03–0.06) mL/g. In FDR, median (range) injected serum, LRS, and combined serum and LRS dose was 0.03 (0.02–0.04), 0.01 (0–0.04), and 0.04 (0.02–0.06) mL/g, respectively. A mild temperature increase (maximum +2.9°C) (MR n = 10, FD n = 3, FDR n = 8) and delayed righting reflex (MR n = 4, FD n = 3, FDR n = 7) occurred in a subset of insects following injection. Two deaths occurred at 2 min (n = 1, FDR) and 96 h (n = 1, FD) post-injection. This is the first report of serum transfusions in thorny devil stick insects, and while largely successful, minor to severe transfusion reactions may occur.}, journal={FRONTIERS IN VETERINARY SCIENCE}, author={Gregory, Taylor M. and Heniff, Ashlyn C. and Gorges, Melinda A. and Lathan, Andrew W. and Lewbart, Gregory A. and Balko, Julie A.}, year={2022}, month={Apr} }
 @article{gregory_hubbard_schlake_mejia_passingham_lewbart_harrison_2022, title={EVALUATION OF PROGNOSTIC INDICATORS FOR INJURED TURTLES PRESENTING TO A WILDLIFE CLINIC}, volume={53}, ISSN={["1937-2825"]}, DOI={10.1638/2020-0200}, abstractNote={Abstract: The Turtle Rescue Team (TRT) at North Carolina State University College of Veterinary Medicine currently sees over 500 chelonian cases a year. Each turtle receives a thorough physical examination as the main evaluation of its health because further diagnostics are cost prohibitive in a donation-based facility. Thus, there is a need to further understand how a few economical diagnostics can evaluate the prognosis of chelonians. The objectives were to evaluate lactate, PCV, total solids, glucose, temperature, and HR as prognostic indicators in multiple chelonian species and to determine how the change in lactate concentrations over a 24 h period affects prognosis. Eastern box turtles (Terrapene carolina carolina), yellow-bellied sliders (Trachemys scripta scripta), river cooters (Pseudemys concinna), painted turtles (Chrysemys picta), and common snapping turtles (Chelydra serpentina) were evaluated because of prevalence of presentation to the TRT, differences in habitat, and differences in physiology. Hyperlactatemia, an increased lactate over 24 h when the initial lactate concentration was greater than 5 mM/L, and anemia were determined to be significant prognostic indicators of mortality for all turtles, and hypoglycemia was a significant prognostic indicator of mortality for male turtles (P < 0.05).}, number={1}, journal={JOURNAL OF ZOO AND WILDLIFE MEDICINE}, author={Gregory, Taylor M. and Hubbard, Cassidy and Schlake, Emily and Mejia, Daniel and Passingham, Kent R. and Lewbart, Gregory A. and Harrison, Tara M.}, year={2022}, month={Mar}, pages={209–213} }
 @article{gregory_harms_gorges_lewbart_papich_2021, title={Pharmacokinetics of ketorolac in juvenile loggerhead sea turtles (Caretta caretta) after a single intramuscular injection}, volume={44}, ISSN={["1365-2885"]}, url={https://doi.org/10.1111/jvp.12952}, DOI={10.1111/jvp.12952}, abstractNote={Abstract}, number={4}, journal={JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS}, author={Gregory, Taylor M. and Harms, Craig A. and Gorges, Melinda A. and Lewbart, Gregory A. and Papich, Mark G.}, year={2021}, month={Jul}, pages={583–589} }