@article{perosa_gris_piva_menegatt_schwertz_sonego_watanabe_pavarini_driemeier_panziera_2024, title={Acute Oral Chromium Exposure Resulting in Ulcerative Gastritis and Perforated Ulcers in Swine}, volume={14}, ISSN={["2076-2615"]}, DOI={10.3390/ani14010063}, abstractNote={Heavy metal poisoning poses a challenge in diagnostic practices and environmental safety. This study describes the epidemiological, clinical, and pathological aspects of a chromium (Cr) poisoning outbreak in growing/finishing pigs housed in pens with bedding of pine wood shavings containing Cr. A visit to the affected farm was conducted. Epidemiological data were collected, and necropsy and histopathological examinations and heavy metal quantifications were performed. Up to 30% of the animals from the affected pens displayed clinical signs 48 h after housing, characterized by apathy, rigid gait, distended abdomen, pain to abdominal palpation, fever, vomiting, and skin cyanosis. The lethality rate reached 76.6%. Main postmortem findings consisted of ulcerative gastritis with perforation of the glandular stomach in all necropsied swine. Heavy metal analysis revealed a higher concentration of Cr in the bedding of the affected pens, along with elevated levels of Cr in the livers of the affected swine. Given that Cr is a known cause of poisoning in humans (with acute oral exposure resulting in corrosive lesions in the gastrointestinal tract), this study marks the first diagnosis of acute oral natural Cr poisoning in animals. This diagnosis was established through the association of epidemiological, pathological, and heavy metal quantification data.}, number={1}, journal={ANIMALS}, author={Perosa, Fernanda Felicetti and Gris, Anderson Hentz and Piva, Manoela Marchezan and Menegatt, Jean Carlo Olivo and Schwertz, Claiton Ismael and Sonego, Paola and Watanabe, Tatiane Terumi Negrao and Pavarini, Saulo Petinatti and Driemeier, David and Panziera, Welden}, year={2024}, month={Jan} }
 @article{frigerio_seraguci_machado_barrantes murillo_negrao watanabe_2024, title={Anorexia, lethargy, weakness, and acute respiratory distress in a 5-year-old male intact mixed-breed dog}, volume={262}, ISSN={["1943-569X"]}, DOI={10.2460/javma.23.09.0498}, number={1}, journal={JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION}, author={Frigerio, Edenilson Dona and Seraguci, Tulio Faria and Machado, Gisele Fabrino and Barrantes Murillo, Daniel Felipe and Negrao Watanabe, Tatiane Terumi}, year={2024}, month={Jan} }
 @article{carnevale_muro_leal_alves_monteiro_gomes_pereira_ferreira_neta_watanabe_et al._2024, title={The effects of feeding sows at onset of farrowing supplemental energy (blend of carbohydrates and glycerol) on farrowing kinetics and piglet vitality}, volume={18}, ISSN={["1751-732X"]}, DOI={10.1016/j.animal.2024.101104}, abstractNote={Delivering piglets is one of the most energy demanding activities sows undergo in their lifetime. Sows can have myometrial contractions from two to 12 hours before the first piglet is expelled as well as a nest building behavior. Thus, when the first piglet is delivered the female has already used part of her energy supply. When the sow gets exhausted due to lack of energy, the farrowing process can be interrupted, causing damage to the viability and vitality of the piglets. In the present study, we evaluated the effects of feeding sows an energy supplement at onset of farrowing on farrowing kinetics and piglet vitality. The energy supplement consisted of a blend of carbohydrates and glycerol which provides 43,9 kJ of metabolizable energy per kg of metabolic weight. A total of 180 sows were used. At onset of farrowing, sows were assigned to one of the following treatments: sows that were not supplied energy at the onset of farrowing, serving as controls (CON, n = 85); sows fed the energy supplement at the onset of farrowing (ESP, n = 95). Farrowing kinetics, blood glucose concentration, and piglet vitality were recorded for each sow. Blood glucose concentration was assessed by puncturing the auricular vein and using a portable glucometer at four different time points: after the birth of the 1st piglet (T0), and at 20 (T20), 40 (T40), 80 (T80), and 180 (T180) minutes after the birth of the 1st piglet. The vitality of the 1st, 6th, 12th, 17th, and 20th piglet born was evaluated using the Apgar score. Piglet birth weight and average colostrum intake were measured. The farrowing duration was 20 min shorter (P < 0.05) for ESP sows in comparison with CON sows. Sows from ESP treatment had higher (P ≤ 0.05) blood glucose concentration at T20 and T40 compared to the CON sows. The inter-piglet birth interval was shorten (P < 0.05) by 14 min between the 1st and 2nd piglet for the ESP treatment. The 17th and 20th piglets born from ESP sows had higher (P < 0.05) Apgar score compared to piglets of the same birth order from CON sows. Colostrum intake was higher (P < 0.01) for piglets born from ESP sows. Litter growth performance did not differ (P > 0.05). In conclusion, feeding a blend of carbohydrates and glycerol as energy supplement for farrowing sows improved farrowing kinetics and piglet vitality score.}, number={3}, journal={ANIMAL}, author={Carnevale, R. F. and Muro, B. B. D. and Leal, D. F. and Alves, L. K. S. and Monteiro, M. S. and Gomes, N. A. C. and Pereira, F. A. and Ferreira, F. N. A. and Neta, C. S. S. and Watanabe, T. T. N. and et al.}, year={2024}, month={Mar} }
 @misc{muro_carnevale_monteiro_yao_ferreira_neta_pereira_maes_janssens_almond_et al._2023, title={A Systematic Review and Meta-Analysis of Creep Feeding Effects on Piglet Pre- and Post-Weaning Performance}, volume={13}, ISSN={["2076-2615"]}, DOI={10.3390/ani13132156}, abstractNote={In the present systematic review and meta-analysis, we evaluated the effects of providing piglets with creep feed during lactation on piglet pre- and post-weaning performance. A total of 20 articles met the inclusion criteria. Creep feeding in lactation improved pre-weaning piglet performance in 46% of the studies selected, while 58% of the included studies reported that creep feeding in lactation improved piglet performance during the nursery phase. Creep feeding increased the average piglet body weight (creep = 7.23 ± 0.30, no creep = 6.96 ± 0.31; p = 0.03) and litter weight (creep = 81.2 ± 4.18, no creep = 76.4 ± 4.22; p < 0.001) at weaning. The average piglet body weight and litter weight were positively associated (p < 0.001 and p < 0.001, respectively) with total creep feed intake. Creep feeding of piglets for more than 14 days increased (p = 0.003) the litter weight at weaning compared to litters not provided or provided for shorter periods with creep feed. The present work strengthened the notion that creep feeding during lactation presents opportunities for improving weaning weights and post-weaning piglet performance compared to litters not provided or provided for shorter periods with creep feed.}, number={13}, journal={ANIMALS}, author={Muro, Bruno B. D. and Carnevale, Rafaella F. and Monteiro, Matheus S. and Yao, Renjie and Ferreira, Felipe N. A. and Neta, Clarice S. S. and Pereira, Francisco A. and Maes, Dominiek and Janssens, Geert P. J. and Almond, Glen W. and et al.}, year={2023}, month={Jul} }
 @misc{rasche_murillo_watanabe_2023, title={Beak fracture associated with leiomyosarcoma in a budgerigar (<i>Melopsittacus undulatus</i>): a case report and literature review}, volume={10}, ISSN={["2297-1769"]}, DOI={10.3389/fvets.2023.1309185}, abstractNote={A 2-year-old male budgerigar (Melopsittacus undulatus) died after a 1-day history of fracture of the rostral rhinotheca with pale mucous membranes, dyspnea, dull mentation, and ataxia. Histopathology revealed an infiltrative neoplasm composed of interweaving streams of spindle cells effacing the dermis and bone of the rostral upper beak as well as a ganglion and two cranial nerves. No visceral metastasis was observed. Neoplastic cells exhibited strong cytoplasmic immunolabeling for alpha-smooth muscle actin (α-SMA) and lacked immunolabeling for S100, Melan-A, PNL2, and cytokeratin AE1/AE3. These findings were consistent with a locally invasive leiomyosarcoma Leiomyosarcomas arise from the smooth muscle and are locally invasive with rare metastases. In birds, leiomyosarcomas are mostly reported to arise from the spleen, gastrointestinal, and reproductive tracts. In the case report herein, we describe the histological and immunohistochemical features of a primary beak leiomyosarcoma in a budgerigar associated with a fracture located at the rostral rhinotheca. Leiomyosarcoma arising from the beak has not been described in the literature.}, journal={FRONTIERS IN VETERINARY SCIENCE}, author={Rasche, Brittany L. and Murillo, Daniel Felipe Barrantes and Watanabe, Tatiane Terumi Negrao}, year={2023}, month={Dec} }
 @article{menegatt_perosa_gris_piva_serena_bordignon_reck_menin_watanabe_driemeier_2023, title={Main Causes of Death in Piglets from Different Brazilian Nursery Farms Based on Clinical, Microbiological, and Pathological Aspects}, volume={13}, ISSN={["2076-2615"]}, DOI={10.3390/ani13243819}, abstractNote={Necropsies can reveal herd problems or comorbidities that can lead to management corrections, improvements in animal performance, and better decision making. Furthermore, the pattern and causes of mortality might differ when different systems are evaluated. The present study was conducted to establish the main causes of death in nursery pigs from different systems in Brazil, as well as the clinical, microbiological, and pathological aspects of these mortalities. Eighteen nurseries were analyzed (a total of 120,243 housed piglets), and 557 necropsies were performed. Streptococcus suis infection was the most prevalent cause of death (21.2%), followed by bacterial polyserositis (16.7%), chronic atrophic enteritis (13.5%), salmonellosis (8.8%), pneumonia (8.6%), and colibacillosis (6.1%). The increase in mortality rate in individual nurseries and, consequently, in the diagnoses was commonly associated with disease outbreaks. Infectious diseases constituted the largest portion of the diagnoses, making a great opportunity for improving production rates in herds. Moreover, the extensive range of observed diagnoses highlights the importance of conducting preliminary diagnostic investigations based on necropsy to determine the causes of death. This approach allows for the direction of complementary tests, which can diagnose agents with greater specificity. As a result, this allows for the implementation of more effective prevention and control strategies.}, number={24}, journal={ANIMALS}, author={Menegatt, Jean Carlo Olivo and Perosa, Fernanda Felicetti and Gris, Anderson Hentz and Piva, Manoela Marchezan and Serena, Guilherme Carvalho and Bordignon, Diego Luiz and Reck, Carolina and Menin, Alvaro and Watanabe, Tatiane Terumi Negrao and Driemeier, David}, year={2023}, month={Dec} }
 @article{molossi_almeida_cecco_pissetti_ventura_brandalise_simao_vanucci_watababe_vaz jr_et al._2023, title={Porcine circovirus type 3: immunohistochemical detection in lesions of naturally affected piglets}, volume={10}, ISSN={["2297-1769"]}, DOI={10.3389/fvets.2023.1174718}, abstractNote={This study aimed to evaluate the relationship between porcine circovirus type 3 (PCV3) viral load and histopathological findings in perinatal piglet tissues and to develop an immunohistochemical method for detecting the virus in lesions. The quantitative polymerase chain reaction (qPCR) cycle threshold (Ct) when amplifying PCV3 DNA and the area of perivascular inflammatory infiltrates in different organs [central nervous system (CNS), lung, heart, liver, spleen, and lymph nodes] were compared. To develop an immunohistochemistry technique, rabbit sera were produced against PCV3-capsid protein peptides selected using bioinformatic analyses. The assay was initially implemented using a tissue sample previously tested using qPCR and in situ hybridization to optimize the procedure and reagent dilutions. To evaluate immunohistochemistry performance, tissue samples from another 17 cases were analyzed using standardized parameters. The most common microscopic lesion was multisystemic periarteritis, with associated vasculitis, as the mesenteric vascular plexus is one of the most affected organs. Other tissues, such as the heart, lung, CNS, and skeletal muscle, were also affected. Comparison of the Ct values for different tissues showed no significant difference, except in lymphoid organs (spleen and lymph nodes), which had significantly higher viral loads than the CNS tissues. There was no correlation between Ct values and perivascular inflammatory infiltrates. PCV3 immunohistochemistry revealed granular immunolabeling, mainly in the cytoplasm of cells in the vascular mesenteric plexus, heart, lung, kidney, and spleen.}, journal={FRONTIERS IN VETERINARY SCIENCE}, author={Molossi, Francieli Adriane and Almeida, Bruno Albuquerque and Cecco, Bianca Santana and Pissetti, Caroline and Ventura, Lauren and Brandalise, Luciano and Simao, Gustavo and Vanucci, Fabio and Watababe, Tatiane Terumi Negrao and Vaz Jr, Itabajara da Silva and et al.}, year={2023}, month={May} }
 @article{murillo_ramaker_burke_walker_negrao watanabe_2023, title={Severe unilateral nephrolithiasis and recurrent colic in a horse}, volume={1}, ISSN={["2052-6121"]}, url={http://dx.doi.org/10.1002/vrc2.567}, DOI={10.1002/vrc2.567}, abstractNote={Abstract}, journal={VETERINARY RECORD CASE REPORTS}, publisher={Wiley}, author={Murillo, Daniel Felipe Barrantes and Ramaker, Erica and Burke, Megan and Walker, Ryan and Negrao Watanabe, Tatiane Terumi}, year={2023}, month={Jan} }
 @article{murillo_watanabe_2023, title={Spontaneous neoplasms in captive red-bellied lemurs (Eulemur rubriventer)}, volume={201}, ISSN={["1532-3129"]}, DOI={10.1016/j.jcpa.2022.12.010}, abstractNote={Red-bellied lemurs (Eulemur rubriventer: genus Eulemur, family Lemuridae, Suborder Strepsirrhini) are non-human primates endemic to the forests of Madagascar and listed as 'vulnerable' on the IUCN Red List of Threatened Species. Currently, descriptions of neoplasia in this species are extremely scarce, with only one case of hepatocellular adenoma reported. Prosimian submissions received by the Anatomic Pathology Service at North Carolina State University from January 2010 to January 2021 were retrieved. A total of 200 cases of Strepsirrhini prosimians were identified, representing 57 (28.5%) individuals from the genus Eulemur of which seven (12.3%) cases were red-bellied lemurs. Neoplasia was identified in two of the seven (28.57%) cases. The first case was a 25-year-old, intact female with hepatocellular carcinoma and the second a 33-year-old, intact female with a cervical mass consistent with osteoblastic osteosarcoma arising from the left clavicular bone. Hepatocellular neoplasms are commonly reported in prosimians, with carcinomas more frequent than adenomas. In contrast, osteosarcoma has been rarely described in lemuriformes, with four cases reported. To our knowledge, this is the first report of osteosarcoma and hepatocellular carcinoma in a red-bellied lemur.}, journal={JOURNAL OF COMPARATIVE PATHOLOGY}, author={Murillo, Daniel F. Barrantes and Watanabe, Tatiane Negrao}, year={2023}, month={Feb}, pages={33–36} }
 @misc{murillo_anderson_capobianco_lewbart_wiederhold_canete-gibas_watanabe_2023, title={Systemic Exophiala equina infection in an Eastern box turtle (Terrapene carolina carolina): a case report and literature review}, volume={10}, ISSN={["2297-1769"]}, DOI={10.3389/fvets.2023.1158393}, abstractNote={Phaeohyphomycosis is an infection caused by melanized fungi. This disease has been reported in several animal species including invertebrates, cold-blooded vertebrates, mammals, and humans. Melanized fungi have similar phenotypical features and confirmation requires culture and molecular diagnostics. To exemplify this we present a case of a 333 g adult of unknown age, free-ranging, male Eastern box turtle (Terrapene carolina carolina) that was referred to the Turtle Rescue Team at North Carolina State University for evaluation of multilobulated masses occupying the entire left orbit and at the right forelimb on the plantarolateral aspect of the foot. A fine needle aspirate cytologic examination of the mass on the right forelimb revealed large numbers of inflammatory cells and fungal organisms. Histopathology of the skin biopsies from the right forefoot was consistent with phaeohyphomycosis. A course of antifungal medication was started (Fluconazole 21 mg/kg loading dose IV then 5 mg/kg PO SID q 30 days). Due to concern for the patient's quality of life and the lack of a curative treatment plan, humane euthanasia was elected. Gross and histological postmortem examination confirmed the presence of multiple coelomic masses similar in appearance to those observed in the left orbit and right forefoot indicating disseminated phaeohyphomycosis. A swab of the periocular mass was submitted for fungal culture and phenotypic identification. The isolate was later identified as Exophiala equina through a combination of phenotypic characterization and sequencing of the ITS region of the nuclear rDNA. Exophiala is a genus in the family Herpotrichiellaceae, order Chaetothyriales and is considered an opportunistic “black yeast” causing infection in aquatic invertebrates, fish, amphibians, reptiles, and mammals including humans. Exophiala equina is infrequently reported in animals, with only three cases in the literature including the herein report.}, journal={FRONTIERS IN VETERINARY SCIENCE}, author={Murillo, Daniel Felipe Barrantes and Anderson, Stephanie and Capobianco, Christian and Lewbart, Gregory A. and Wiederhold, Nathan P. and Canete-Gibas, Connie F. and Watanabe, Tatiane Terumi Negrao}, year={2023}, month={May} }
 @article{anderson_murillo_womble_gibbs_harrell_watanabe_2022, title={Case Report: Novel Disseminated Paecilomyces formosus Infection in a Dog}, volume={9}, ISSN={["2297-1769"]}, DOI={10.3389/fvets.2022.878327}, abstractNote={A 2.5-year-old, 25.5 kg, spayed female Australian Shepherd dog had a 2-month history of shifting leg lameness in all limbs, tetraparesis, progressive lethargy, and severe pain. On the physical examination, fever (40.61°C), tachycardia, tachypnea, mild diffuse pelvic limb muscular atrophy, left prescapular and right popliteal lymphadenomegaly were observed. Due to the poor prognosis and difficult pain management, humane euthanasia was elected. Macroscopic and histological findings revealed multifocal to coalescing granulomas with central areas of lytic necrosis within the right femur, left humerus, left scapula, left biceps brachii, right semimembranosus muscle, liver, spleen, and lymph nodes. The necrotic areas contained myriad intralesional, intracellular, and extracellular negatively stained, non-pigmented, septate acute angle branching hyphae with parallel walls measuring 3–6 μm in width with polar bulbous projections measuring 7–13 μm in width. Fresh samples of the liver were submitted for fungal culture. Panfungal PCR targeting the major conserved genes-ITS, TUB, CAL-confirmed Paecilomyces formosus. Paecilomyces spp. are members of anamorphic fungi classified under the phylum Ascomycota. Paecilomycosis is an uncommon fungal infection caused by Paecilomyces spp with a disease reported in humans and animals ranging from superficial to systemic clinical forms affecting both immunocompromised and immunocompetent individuals. In dogs, disseminated paecilomycosis has been reported, but the species of fungi are not always determined. To our knowledge, this is the first case of disseminated paecilomycosis caused by P. formosus infection in a dog.}, journal={FRONTIERS IN VETERINARY SCIENCE}, author={Anderson, Stephanie and Murillo, Daniel Felipe Barrantes and Womble, Mandy and Gibbs, Nicole and Harrell, Karyn and Watanabe, Tatiane Terumi Negrao}, year={2022}, month={May} }
 @article{leal_viana_almond_monteiro_garbossa_carnevale_muro_poor_pugliesi_nichi_et al._2022, title={Estrus Synchronization of Replacement Gilts Using Estradiol Cipionate and PGF(2 alpha) and Its Effects on Reproductive Outcomes}, volume={12}, ISSN={["2076-2615"]}, DOI={10.3390/ani12233393}, abstractNote={In this study, we evaluated the effectiveness of using estrogen-induced prolonged luteal function followed by prostaglandin F2 alpha (PGF2α) treatment to synchronize estrus in gilts. On day12 of the estrus cycle (D0 = first day of standing estrus), 52 gilts were assigned at random to two experimental groups: non-treated gilts (CON, n = 22), serving as controls, and prolonged luteal function group (CYP, n = 30), receiving a single treatment with 10 mg of estradiol cypionate intramuscularly Starting on day 12, blood samples were collected for estradiol and progesterone assays. Estrus detection started on day 17. Gilts from the CON group were inseminated at the onset of natural estrus. On day 28 CYP gilts were treated with PGF2α to induce luteolysis and inseminated at the onset of estrus. Gilts were slaughtered 5 d after the last insemination. A single treatment with estradiol cypionate prolonged luteal function in 90% of treated gilts. The duration of the estrous cycle was longer (p < 0.0001) for CYP gilts compared to CON gilts. CYP gilts showed synchronized estrus 3.96 ± 0.19 d after induction of luteolysis. The conception rate was similar (p = 0.10) for CON and CYP gilts. No difference was observed in the embryo recovery rate (p = 0.18) and total number of embryos per female (p = 0.06). The percentage of unfertilized oocytes, fragmented embryos and viable embryos was similar among females from CON and CYP groups (p > 0.05). The treatment of gilts with a single application of 10 mg of estradiol cypionate on day 12 of the estrous cycle was effective in prolonging luteal function and treatment with PGF2α resulted in synchronized estrus. Additionally, the synchronization protocol had no deleterious effect on fertility and embryonic development.}, number={23}, journal={ANIMALS}, author={Leal, Diego Feitosa and Viana, Carlos Henrique Cabral and Almond, Glen William and Monteiro, Matheus Saliba and Garbossa, Cesar Augusto Pospissil and Carnevale, Rafaella Fernandes and Muro, Bruno Bracco Donatelli and Poor, Andre Pegoraro and Pugliesi, Guilherme and Nichi, Marcilio and et al.}, year={2022}, month={Dec} }
 @article{proctor_wolf_brodsky_cortes_frias-de-diego_almond_crisci_negrão watanabe_hammer_käser_2022, title={Heterologous vaccine immunogenicity, efficacy, and immune correlates of protection of a modified-live virus porcine reproductive and respiratory syndrome virus vaccine}, volume={13}, ISSN={1664-302X}, url={http://dx.doi.org/10.3389/fmicb.2022.977796}, DOI={10.3389/fmicb.2022.977796}, abstractNote={Although porcine reproductive and respiratory syndrome virus (PRRSV) vaccines have been available in North America for almost 30 years, many vaccines face a significant hurdle: they must provide cross-protection against the highly diverse PRRSV strains. This cross-protection, or heterologous vaccine efficacy, relies greatly on the vaccine’s ability to induce a strong immune response against various strains—heterologous immunogenicity. Thus, this study investigated vaccine efficacy and immunogenicity of a modified live virus (MLV) against four heterologous type 2 PRRSV (PRRSV-2) strains. In this study, 60 pigs were divided into 10 groups. Half were MOCK-vaccinated, and the other half vaccinated with the Prevacent® PRRS MLV vaccine. Four weeks after vaccination, groups were challenged with either MOCK, or four PRRSV-2 strains from three different lineages—NC174 or NADC30 (both lineage 1), VR2332 (lineage 5), or NADC20 (lineage 8). Pre-and post-challenge, lung pathology, viral loads in both nasal swabs and sera, anti-PRRSV IgA/G, neutralizing antibodies, and the PRRSV-2 strain-specific T-cell response were evaluated. At necropsy, the lung samples were collected to assess viral loads, macroscopical and histopathological findings, and IgA levels in bronchoalveolar lavage. Lung lesions were only induced by NC174, NADC20, and NADC30; within these, vaccination resulted in lower gross and microscopic lung lesion scores of the NADC20 and NADC30 strains. All pigs became viremic and vaccinated pigs had decreased viremia upon challenge with NADC20, NADC30, and VR2332. Regarding vaccine immunogenicity, vaccination induced a strong systemic IgG response and boosted the post-challenge serum IgG levels for all strains. Furthermore, vaccination increased the number of animals with neutralizing antibodies against three of the four challenge strains—NADC20, NADC30, and VR2332. The heterologous T-cell response was also improved by vaccination: Not only did vaccination increase the induction of heterologous effector/memory CD4 T cells, but it also improved the heterologous CD4 and CD8 proliferative and/or IFN-γ response against all strains. Importantly, correlation analyses revealed that the (non-PRRSV strain-specific) serum IgG levels and the PRRSV strain-specific CD4 T-cell response were the best immune correlates of protection. Overall, the Prevacent elicited various degrees of efficacy and immunogenicity against four heterologous and phylogenetically distant strains of PRRSV-2.}, journal={Frontiers in Microbiology}, publisher={Frontiers Media SA}, author={Proctor, Jessica and Wolf, Iman and Brodsky, David and Cortes, Lizette M. and Frias-De-Diego, Alba and Almond, Glen W. and Crisci, Elisa and Negrão Watanabe, Tatiane Terumi and Hammer, James M. and Käser, Tobias}, year={2022}, month={Sep} }
 @article{silva_toedoro_wouters_wouters_abreu_neto_watanabe_2022, title={Metastatic Digital Chondrogenic Melanocytic Tumour in a Dog}, volume={190}, ISSN={["1532-3129"]}, DOI={10.1016/j.jcpa.2021.10.010}, abstractNote={A 9-year-old intact male Brazilian Mastiff dog with a 1-year history of progressive severe swelling in all four limbs and significant locomotory difficulty was euthanized due to unresponsiveness to medical management. Macroscopically, the distal phalanx of the 5th digit of the left hindlimb was replaced by a cutaneous, non-ulcerated, 3.0 cm diameter, multilobulated, black, firm nodule. The cortical bones of the appendicular skeleton were thickened and partially effaced by infiltrative coalescing nodules (0.1-1.0 cm diameter). The lungs, heart, pleura, mesentery, adrenal glands and kidneys were infiltrated by similar nodules (0.5-3.5 cm diameter). Histological evaluation of the masses revealed a non-encapsulated malignant neoplasm composed of spindloid to polygonal cells that contained variable amounts of intracytoplasmic melanin and were arranged in haphazardly interlacing streams and bundles supported by scant fibrovascular stroma with myxoid and chondroid matrix formation. Neoplastic cells had intense cytoplasmic labeling for S100 and 10% had moderate cytoplasmic immunoreactivity for Melan-A. To the authors' knowledge, this is the first description of a canine metastatic digital chondrogenic melanocytic tumour with strongly suggestive chondroid differentiation of neoplastic melanocytes.}, journal={JOURNAL OF COMPARATIVE PATHOLOGY}, author={Silva, Laice A. and Toedoro, Tamires G. W. and Wouters, Angelica T. B. and Wouters, Flademir and Abreu, Daniela S. and Neto, Daniel M. G. P. and Watanabe, Tatiane T. Negrao}, year={2022}, month={Jan}, pages={13–18} }
 @article{womble_cabot_harrison_watanabe_2022, title={Outbreak in African lions of Yersinia pseudotuberculosis infection, with aberrant bacterial morphology}, volume={1}, ISSN={["1943-4936"]}, DOI={10.1177/10406387211072822}, abstractNote={ A concurrent outbreak of infection with Yersinia pseudotuberculosis occurred in adult captive African lions ( Panthera leo). Two 17-y-old male lions and one 14-y-old female lion developed respiratory distress, lethargy, ataxia, and hyporexia. Within 3–5 d of the onset of clinical signs, one male and the female lion died and were submitted for postmortem examination. Macroscopically, the liver and spleen had multifocal-to-coalescing, semi-firm, pale-tan nodules throughout the parenchyma. The lungs were non-collapsed and marked by petechiae. Histologic examination identified lytic, necrosuppurative foci in the liver, spleen, lungs, and kidney, with abundant intralesional gram-negative coccobacilli in the male lion. Similar findings were seen in the female lion in the liver, spleen, kidney, and mesenteric lymph node; however, the intralesional bacterial colonies were more pleomorphic, comprising rod and filamentous morphologies. Aerobic bacterial culture of the liver, spleen, and lung revealed Y. pseudotuberculosis growth. The source of infection is unknown, and an epidemiologic study was performed. Sources to be considered are from the predation of rodent and/or bird reservoirs, or contaminated soil or water. Mortality associated with Y. pseudotuberculosis has been described in an African lion cub, however, to our knowledge, Y. pseudotuberculosis has not been reported in adult African lions, and this is only the second report of Y. pseudotuberculosis with aberrant bacterial morphology observed histologically. }, journal={JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION}, author={Womble, Mandy and Cabot, Megan L. and Harrison, Tara and Watanabe, Tatiane Terumi Negrao}, year={2022}, month={Jan} }
 @article{rasche_parker_lewbart_petritz_watanabe_2022, title={Pathology in Practice}, volume={260}, ISSN={["1943-569X"]}, DOI={10.2460/javma.21.07.0328}, number={3}, journal={JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION}, author={Rasche, Brittany L. and Parker, Maryanna and Lewbart, Gregory A. and Petritz, Olivia and Watanabe, Tatiane Terumi Negrao}, year={2022}, month={Feb}, pages={305–307} }
 @article{souza teixeira campos_barrantes murillo_roessner_ruff_negrao watanabe_2022, title={Pathology in Practice}, volume={260}, ISSN={["1943-569X"]}, DOI={10.2460/javma.22.03.0100}, number={11}, journal={JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION}, author={Souza Teixeira Campos, Maria Eduarda and Barrantes Murillo, Daniel Felipe and Roessner, Holly and Ruff, Jennifer and Negrao Watanabe, Tatiane Terumi}, year={2022}, month={Aug}, pages={1305–1307} }
 @article{bauer_murillo_schreeg_borst_watanabe_2022, title={Pathology in Practice}, volume={260}, ISSN={["1943-569X"]}, DOI={10.2460/javma.22.03.0119}, number={12}, journal={JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION}, author={Bauer, Katherine and Murillo, Daniel Felipe Barrantes and Schreeg, Megan E. and Borst, Luke B. and Watanabe, Tatiane Terumi Negrao}, year={2022}, month={Sep}, pages={1466–1468} }
 @article{cabot_watanabe_womble_harrison_2022, title={YERSINIA PSEUDOTUBERCULOSIS INFECTION IN LIONS (PANTHERA LEO) AT A ZOOLOGICAL PARK}, volume={53}, ISSN={["1937-2825"]}, DOI={10.1638/2021-0106}, abstractNote={Abstract: Two co-housed 17-yr-old male lions (Panthera leo) and one 15-yr-old female lion in an adjacent enclosure developed acute lethargy, depression, anorexia, and ataxia at a zoological park in central North Carolina, United States. One of the male lions and the female lion were found dead 5 and 4 d after the onset of clinical signs, respectively. The other male lion recovered without any clinical treatment. A third male lion housed with the female lion never developed clinical signs. Postmortem examination, microscopic evaluation, and bacterial culture of the liver from both deceased lions confirmed systemic Yersinia pseudotuberculosis infection. Susceptibility testing revealed resistance to amoxicillin–clavulanic acid and cefazolin. To investigate the extent of the outbreak, qualitative real-time polymerase chain reaction (PCR) for Y. pseudotuberculosis was performed on feces and substrate from 15 enclosures housing nondomestic felids and canids, resulting in a positive sample from one enclosure housing four asymptomatic lions. This enclosure was adjacent to housing of the deceased female lion. Enrofloxacin was administered to all animals in the vicinity at doses of 5 mg/kg in nondomestic felids and 10 mg/kg in nondomestic canids, orally q24h for 14 d. Repeated fecal PCR performed 1 wk after completing the antibiotic treatment protocol found no positive samples. The source of the infection was not identified despite PCR testing of environmental samples from all enclosures in the vicinity, the remains of a prey item fed out prior to the outbreak, and a single dead rodent found on grounds. No further clinical cases have occurred within the following year.}, number={3}, journal={JOURNAL OF ZOO AND WILDLIFE MEDICINE}, author={Cabot, Megan L. and Watanabe, Tatiane Terumi Negrao and Womble, Mandy and Harrison, Tara M.}, year={2022}, month={Sep}, pages={593–599} }
 @article{rasche_tucker_linder_harrison_negrao watanabe_2021, title={Case Report: Pulmonary Conidiobolomycosis in a Vietnamese Pot-Bellied Pig}, volume={8}, ISSN={["2297-1769"]}, DOI={10.3389/fvets.2021.799641}, abstractNote={An adult castrated male Vietnamese pot-bellied pig had a 1-week history of acute dyspnea and lethargy. Minimal diagnostic testing was authorized by the owner, resulting in treatment with a third-generation cephalosporin and a non-steroidal anti-inflammatory drug. Partial improvement was observed after a week; however, the pig died 2 weeks after the initial onset of clinical signs. Macroscopically, ~90% of the left lung was effaced by large masses with a caseonecrotic center. Histologic examination revealed eosinophilic granulomas with myriad, intralesional, negatively staining hyphae highlighted by “sleeves” of hypereosinophilic material (Splendore-Hoeppli material). Infection with an oomycete or “zygomycete” (i.e., organisms of the order Entomophthorales or Mucorales) was initially considered. Pan-fungal PCR and sequencing performed on formalin-fixed, paraffin-embedded lung tissue identified Conidiobolus spp., consistent with a diagnosis of primary pulmonary conidiobolomycosis. There are only a few reports of infections with Conidiobolus spp. (and other members of the order Entomophthorales) in swine. Unlike humans and other animal species, conidiobolomycosis in pigs presents more commonly as a primary pulmonary disease rather than rhinofacial or nasopharyngeal disease.}, journal={FRONTIERS IN VETERINARY SCIENCE}, author={Rasche, Brittany L. and Tucker, Samuel M. and Linder, Keith and Harrison, Tara M. and Negrao Watanabe, Tatiane Terumi}, year={2021}, month={Dec} }
 @article{womble_schreeg_hoch_meira_foster_premanandan_watanabe_2021, title={Concurrent Clostridial Enteritis and Oviductal Adenocarcinoma with Carcinomatosis in an Adult Alpaca (Vicugna pacos)}, volume={189}, ISSN={["1532-3129"]}, DOI={10.1016/j.jcpa.2021.09.007}, abstractNote={An adult alpaca (Vicugna pacos) with a history of colic and anorexia was euthanized because of failure to respond to treatment. Macroscopically, pale-tan, multifocal to coalescing, firm nodules and plaques markedly expanded the omentum, mesentery and the parietal and visceral peritoneum of multiple abdominal organs, especially the right oviduct and associated mesosalpinx. Abundant dark-red watery digesta were present in the duodenum and jejunum. Histological evaluation of the right oviduct, abdominal visceral nodules and plaques and mesenteric lymph nodes revealed transmural expansion and replacement by an epithelial malignant neoplasm, comprised of tubules and acini of ciliated columnar cells supported by abundant fibrous connective tissue. Both ovaries were histologically normal. On the basis of the ciliated morphology of the neoplastic cells, the focus on the proximal reproductive tract and the unremarkable ovaries, a reproductive tubal adenocarcinoma with carcinomatosis was diagnosed, with both the endometrium and oviduct considered as the tissues of origin. The prominent ciliated morphology of the neoplastic cells and the classification of human fallopian tube (oviduct) neoplasia lead us to propose oviductal adenocarcinoma with widespread carcinomatosis as the definitive diagnosis. The lamina propria of the small intestine was infiltrated segmentally by lymphocytes, plasma cells and neutrophils, and Clostridium perfringens with beta2 toxin production was identified by polymerase chain reaction in the small intestinal contents. To our knowledge, this is the first report of these two distinct diseases in an alpaca.}, journal={JOURNAL OF COMPARATIVE PATHOLOGY}, author={Womble, Mandy and Schreeg, Megan E. and Hoch, Allison and Meira, Enoch B. de Souza, Jr. and Foster, Derek and Premanandan, Christopher and Watanabe, Tatiane T. Negrao}, year={2021}, month={Nov}, pages={52–58} }
 @article{moura_sampaio_kobayashi_laufer-amorim_ferreira_watanabe_fonseca-alves_2021, title={Structural and Ultrastructural Morphological Evaluation of Giant Anteater (Myrmecophaga tridactyla) Prostate Gland}, volume={10}, ISSN={["2079-7737"]}, DOI={10.3390/biology10030231}, abstractNote={The giant anteater (Myrmecophaga tridactyla) is a vulnerable species from Central and South America, and is considered possibly extinct in Belize, Guatemala, El Salvador, and Uruguay. Due to the species’ conservation and reproductive importance, this research aimed to characterize the morphology, histochemical, immunohistochemical, and ultrastructural feature of the giant anteater prostate gland. For this, we collected 11 giant anteater prostate glands and performed macroscopic, morphological, histochemical, immunohistochemical, and ultrastructural analysis. Nine prostate glands from an adult subject and two from young subjects were studied. Grossly, the adult giant anteater prostate gland is divided in two distinct zones; the central zones (composed mainly of ducts) and the peripheral zones (of acini formed by secretory cells). The secretory cells showed positive periodic acid–Schiff staining. Furthermore, the immunohistochemical characterization revealed a similar human prostate pattern, with p63 staining basal cells, uroplakin III (UPIII) superficial cells of prostatic urethra, androgen receptor (AR) expressing nucleus of secretory and stromal cells, and prostatic specific antigen (PSA) staining prostatic epithelial cells. Overall, our research provided an in-depth morphological description of the giant anteater’s prostate gland, providing valuable information for futures studies focused on giant anteater conservation.}, number={3}, journal={BIOLOGY-BASEL}, author={Moura, Fernanda and Sampaio, Leticia and Kobayashi, Priscila and Laufer-Amorim, Renee and Ferreira, Joao Carlos and Watanabe, Tatiane Terumi Negrao and Fonseca-Alves, Carlos E.}, year={2021}, month={Mar} }