Todd Robert Klaenhammer

A double-blind, 377-subject randomized study identifies Ruminococcus, Coprococcus, Christensenella, and Collinsella as long-term potential key players in the modulation of the gut microbiome of lactose intolerant individuals by galacto-oligosaccharides

GUT MICROBES, 13(1).

In Vivo Transcriptome of Lactobacillus acidophilus and Colonization Impact on Murine Host Intestinal Gene Expression

MBIO, 12(1).

Get Cultured: Eat Bacteria

ANNUAL REVIEW OF FOOD SCIENCE AND TECHNOLOGY, VOL 10, Vol. 10, pp. 1–20.

Deletion-based escape of CRISPR-Cas9 targeting in Lactobacillus gasseri

MICROBIOLOGY-SGM, 164(9), 1098–1111.

Nod2 is required for antigen-specific humoral responses against antigens orally delivered using a recombinant Lactobacillus vaccine platform

PLOS ONE, 13(5).

Phenotypic and genotypic diversity of Lactobacillus buchneri strains isolated from spoiled, fermented cucumber

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 280, 46–56.

An Extracellular Cell-Attached Pullulanase Confers Branched alpha-Glucan Utilization in Human Gut Lactobacillus acidophilus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(12).

CRISPR-Cas Technologies and Applications in Food Bacteria

ANNUAL REVIEW OF FOOD SCIENCE AND TECHNOLOGY, VOL 8, Vol. 8, pp. 413–437.

Deletion of Lipoteichoic Acid Synthase Impacts Expression of Genes Encoding Cell Surface Proteins in Lactobacillus acidophilus

Frontiers in Microbiology, 8.

Lactobacillus acidophilus Metabolizes Dietary Plant Glucosides and Externalizes Their Bioactive Phytochemicals

MBIO, 8(6).

The S-layer Associated Serine Protease Homolog PrtX Impacts Cell Surface-Mediated Microbe-Host Interactions of Lactobacillus acidophilus NCFM

FRONTIERS IN MICROBIOLOGY, 8.

AcmB Is an S-Layer-Associated beta-N-Acetylglucosaminidase and Functional Autolysin in Lactobacillus acidophilus NCFM

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(18), 5687–5697.

Conserved S-Layer-Associated Proteins Revealed by Exoproteomic Survey of S-Layer-Forming Lactobacilli

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(1), 134–145.

Development of a Transportation System Simulation Manual Framework From Theory to Practice

TRANSPORTATION RESEARCH RECORD, (2561), 45–52.

Differential proteome and cellular adhesion analyses of the probiotic bacterium Lactobacillus acidophilus NCFM grown on raffinose - an emerging prebiotic

PROTEOMICS, 16(9), 1361–1375.

Functional Analysis of an S-Layer-Associated Fibronectin-Binding Protein in Lactobacillus acidophilus NCFM

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(9), 2676–2685.

Genomic evolution of lactic acid bacteria: From single gene function to the pan-genome

Biotechnology of Lactic Acid Bacteria: Novel Applications, 2nd Edition, 32–54.

Intracellular and Extracellular Expression of Bacillus thuringiensis Crystal Protein Cry5B in Lactococcus lactis for Use as an Anthelminthic

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(4), 1286–1294.

Multivalent Chromosomal Expression of the Clostridium botulinum Serotype A Neurotoxin Heavy-Chain Antigen and the Bacillus anthracis Protective Antigen in Lactobacillus acidophilus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(20), 6091–6101.

Stuck in the Middle: Fibronectin-Binding Proteins in Gram-Positive Bacteria

[Review of ]. FRONTIERS IN MICROBIOLOGY, 7.

CRISPR-based screening of genomic island excision events in bacteria

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 112(26), 8076–8081.

Expanding the biotechnology potential of lactobacilli through comparative genomics of 213 strains and associated genera

NATURE COMMUNICATIONS, 6.

Genetic Mechanisms of Prebiotic Oligosaccharide Metabolism in Probiotic Microbes

ANNUAL REVIEW OF FOOD SCIENCE AND TECHNOLOGY, VOL 6, Vol. 6, pp. 137–156.

Mucosal Immunogenicity of Genetically Modified Lactobacillus acidophilus Expressing an HIV-1 Epitope within the Surface Layer Protein

PLOS ONE, 10(10).

Occurrence and activity of a type II CRISPR-Cas system in Lactobacillus gasseri

MICROBIOLOGY-SGM, 161, 1752–1761.

SIGNR3-dependent immune regulation by Lactobacillus acidophilus surface layer protein A in colitis

EMBO JOURNAL, 34(7), 881–895.

Sortase-deficient lactobacilli: effect on immunomodulation and gut retention

Call, E. K., Goh, Y. J., Selle, K., Klaenhammer, T. R., & O'Flaherty, S. (2015, February). MICROBIOLOGY-SGM, Vol. 161, pp. 311–321.

Effects of genetic, processing, or product formulation changes on efficacy and safety of probiotics

ANNALS REPORTS, Vol. 1309, pp. 1–18.

Impact of genomics on the field of probiotic research: historical perspectives to modern paradigms

[Review of ]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 106(1), 141–156.

Insights into glycogen metabolism in Lactobacillus acidophilus: impact on carbohydrate metabolism, stress tolerance and gut retention

[Review of ]. MICROBIAL CELL FACTORIES, 13.

Microbiology bacteria get vaccinated

Nature, 513(7517), 175–176.

Recent insight in alpha-glucan metabolism in probiotic bacteria

BIOLOGIA, 69(6), 713–721.

Spontaneously Induced Prophages in Lactobacillus gasseri Contribute to Horizontal Gene Transfer

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 80(11), 3508–3517.

A functional glycogen biosynthesis pathway in Lactobacillus acidophilus: expression and analysis of the glg operon

MOLECULAR MICROBIOLOGY, 89(6), 1187–1200.

Genomic and phenotypic evidence for probiotic influences of Lactobacillus gasseri on human health

[Review of ]. FEMS MICROBIOLOGY REVIEWS, 37(6), 915–935.

Identification of extracellular surface-layer associated proteins in Lactobacillus acidophilus NCFM

MICROBIOLOGY-SGM, 159, 2269–2282.

Improving lactose digestion and symptoms of lactose intolerance with a novel galacto-oligosaccharide (RP-G28): a randomized, double-blind clinical trial

NUTRITION JOURNAL, 12.

Probiotics, prebiotics, and the host microbiome: the science of translation

ANNALS REPORTS, 1306, 1–17.

Recent insight into oligosaccharide uptake and metabolism in probiotic bacteria

[Review of ]. BIOCATALYSIS AND BIOTRANSFORMATION, 31(4), 226–235.

Relevance and application of sortase and sortase-dependent proteins in lactic acid bacteria

[Review of ]. FRONTIERS IN MICROBIOLOGY, 4.

Transcriptional analysis of oligosaccharide utilization by Bifidobacterium lactis Bl-04

BMC GENOMICS, 14(1).

Abating colon cancer polyposis by Lactobacillus acidophilus deficient in lipoteichoic acid

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109(26), 10462–10467.

Characterization of the fecal microbiota using high-throughput sequencing reveals a stable microbial community during storage

PLoS One, 7(10).

Construction and Immunological Evaluation of Dual Cell Surface Display of HIV-1 Gag and Salmonella enterica Serovar Typhimurium FliC in Lactobacillus acidophilus for Vaccine Delivery

CLINICAL AND VACCINE IMMUNOLOGY, 19(9), 1374–1381.

Influence of Exposure Time on Gene Expression by Human Intestinal Epithelial Cells Exposed to Lactobacillus acidophilus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 78(14), 5028–5032.

Raffinose family oligosaccharide utilisation by probiotic bacteria: insight into substrate recognition, molecular architecture and diversity of GH36 alpha-galactosidases

BIOCATALYSIS AND BIOTRANSFORMATION, 30(3), 316–325.

The impact of probiotics and prebiotics on the immune system

NATURE REVIEWS IMMUNOLOGY, 12(10), 728–734.

Transcriptional Analysis of Prebiotic Uptake and Catabolism by Lactobacillus acidophilus NCFM

PLOS ONE, 7(9).

A rendezvous with our microbes

Gordon, J. I., & Klaenhammer, T. R. (2011, March 15). PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol. 108, pp. 4513–4515.

Assessment of Lactobacillus gasseri as a Candidate Oral Vaccine Vector

CLINICAL AND VACCINE IMMUNOLOGY, 18(11), 1834–1844.

Directed Chromosomal Integration and Expression of the Reporter Gene gusA3 in Lactobacillus acidophilus NCFM

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 77(20), 7365–7371.

Dissimilar Properties of Two Recombinant Lactobacillus acidophilus Strains Displaying Salmonella FliC with Different Anchoring Motifs

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 77(18), 6587–6596.

Group-specific comparison of four lactobacilli isolated from human sources using differential blast analysis

GENES AND NUTRITION, 6(3), 319–340.

Invited review: Application of omics tools to understanding probiotic functionality

[Review of ]. JOURNAL OF DAIRY SCIENCE, 94(10), 4753–4765.

Probiotic and prebiotic claims in Europe: seeking a clear roadmap

Guarner, F., Sanders, M. E., Gibson, G., Klaenhammer, T., Cabana, M., Scott, K., … Hill, C. (2011, December).

Regulation of induced colonic inflammation by Lactobacillus acidophilus deficient in lipoteichoic acid

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 108, 4623–4630.

The Impact of Omic Technologies on the Study of Food Microbes

ANNUAL REVIEW OF FOOD SCIENCE AND TECHNOLOGY, VOL 2, Vol. 2, pp. 353–371.

Transcriptional and functional analysis of galactooligosaccharide uptake by lacS in Lactobacillus acidophilus

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 108(43), 17785–17790.

Construction of vectors for inducible and constitutive gene expression in Lactobacillus

Microbial Biotechnology, 4(3), 357–367.

Functional Roles of Aggregation-Promoting-Like Factor in Stress Tolerance and Adherence of Lactobacillus acidophilus NCFM

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76(15), 5005–5012.

Functional and phenotypic characterization of a protein from Lactobacillus acidophilus involved in cell morphology, stress tolerance and adherence to intestinal cells

MICROBIOLOGY-SGM, 156, 3360–3367.

Genomic Evolution of Domesticated Microorganisms

ANNUAL REVIEW OF FOOD SCIENCE AND TECHNOLOGY, VOL 1, Vol. 1, pp. 397–414.

Plasmid Transduction Using Bacteriophage Phi adh for Expression of CC Chemokines by Lactobacillus gasseri ADH

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76(12), 3878–3885.

Targeted expression of anthrax protective antigen by Lactobacillus gasseri as an anthrax vaccine

FUTURE MICROBIOLOGY, 5(8), 1289–1296.

The role and potential of probiotic bacteria in the gut, and the communication between gut microflora and gut/host

O'Flaherty, S., & Klaenhammer, T. R. (2010, April). INTERNATIONAL DAIRY JOURNAL, Vol. 20, pp. 262–268.

Compositions comprising promoter sequences and methods of use

Dendritic cell targeting of Bacillus anthracis protective antigen expressed by Lactobacillus acidophilus protects mice from lethal challenge

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 106(11), 4331–4336.

Development and Application of a upp-Based Counterselective Gene Replacement System for the Study of the S-Layer Protein SlpX of Lactobacillus acidophilus NCFM

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(10), 3093–3105.

Genome-scale analyses of health-promoting bacteria: probiogenomics

[Review of ]. NATURE REVIEWS MICROBIOLOGY, 7(1), 61–U77.

Genomic features of Lactobacillus species

[Review of ]. Frontiers in Bioscience, 14, 1362–1386.

Genomics of lactic acid bacteria

[Review of ]. FEMS MICROBIOLOGY LETTERS, 292(1), 1–6.

Immunogenicity of Lactobacillus gasseri-FliC as an oral mucosal vaccine adjuvant for HIV

RETROVIROLOGY, Vol. 6.

Lactobacillus acidophillus nucleic acid sequences encoding cell surface protein homologues and uses therefore

Lactobacillus acidophilus nucleic acid sequences encoding stress-related proteins and uses therefor

Nucleic acid sequences encoding two-component sensing and regulatory proteins, antimicrobial proteins and uses therefor

Probiotics to minimize the disruption of faecal microbiota in healthy subjects undergoing antibiotic therapy

JOURNAL OF MEDICAL MICROBIOLOGY, 58(5), 663–670.

Role of Transporter Proteins in Bile Tolerance of Lactobacillus acidophilus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(18), 6013–6016.

Role of autoinducer-2 on the adhesion ability of Lactobacillus acidophilus

JOURNAL OF APPLIED MICROBIOLOGY, 107(1), 269–279.

Temporal gene expression and probiotic attributes of Lactobacillus acidophilus during growth in milk

JOURNAL OF DAIRY SCIENCE, 92(3), 870–886.

Toll-like receptor activation profiles of wild-type, recombinant, and mutant Lactobacillus: implications for vaccine design

RETROVIROLOGY, Vol. 6.

Analysis of the genome sequence of Lactobacillus gasseri ATCC 33323 reveals the molecular basis of an autochthonous intestinal organism

Azcarate-Peril, M. A., Altermann, E., Goh, Y. J., Tallon, R., Sanozky-Dawes, R. B., Pfeiler, E. A., … Klaenhammer, T. R. (2008, August). APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol. 74, pp. 4610–4625.

Functional genomics of probiotic Lactobacilli

Klaenhammer, T. R., Altermann, E., Pfeiler, E., Buck, B. L., Goh, Y.-J., O'Flaherty, S., … Duong, T. (2008, September). JOURNAL OF CLINICAL GASTROENTEROLOGY, Vol. 42, pp. S160–S162.

Genome sequence and characteristics of Lrm1, a prophage from industrial Lactobacillus rhamnosus strain M1

Durmaz, E., Miller, M. J., Azcarate-Peril, M. A., Toon, S. P., & Klaenhammer, T. R. (2008, August). APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol. 74, pp. 4601–4609.

Lactobacillus acidophilus nucleic acid sequences encoding carbohydrate utilization-related proteins and uses therefor

Lactobacillus acidophilus nucleic acid sequences encoding cell surface protein homologues and uses therefore

Washington, DC: U.S. Patent and Trademark Office.

Lactobacillus acidophilus nucleic acid sequences encoding protease homologues and uses therefore

Lactobacillus acidophilus nucleic acids and uses thereof

S layer protein A of Lactobacillus acidophilus NCFM regulates immature dendritic cell and T cell functions

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 105(49), 19474–19479.

Specific Lactobacillus species differentially activate Toll-like receptors and downstream signals in dendritic cells

EXPERT REVIEW OF VACCINES, 7(8), 1155–1164.

Targeting mucosal dendritic cells with microbial antigens from probiotic lactic acid bacteria

[Review of ]. EXPERT REVIEW OF VACCINES, 7(2), 163–174.

AbiZ phage resistance gene

Washington, DC: U.S. Patent and Trademark Office.

Abortive phage resistance mechanism AbiZ speeds the lysis clock to cause premature lysis of phage-infected Lactococcus lactis

JOURNAL OF BACTERIOLOGY, 189(4), 1417–1425.

Anti-inflammatory properties of Lactobacillus gasseri expressing manganese superoxide dismutase using the interleukin 10-deficient mouse model of colitis

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 293(4), G729–G738.

Characterization of a novel bile-inducible operon encoding a two-component regulatory system in Lactobacillus acidophilus

JOURNAL OF BACTERIOLOGY, 189(13), 4624–4634.

Identification of an operon and inducing peptide involved in the production of lactacin B by Lactobacillus acidophilus

JOURNAL OF APPLIED MICROBIOLOGY, 103(5), 1766–1778.

Influence of the dairy environment on gene expression and substrate(1-3)

Klaenhammer, T. R., Azcarate-Peril, M. A., Altermann, E., & Barrangou, R. (2007, March). JOURNAL OF NUTRITION, Vol. 137, pp. 748S–750S.

Inhibition of bacteriophage replication in Streptococcus thermophilus by subunit poisoning of primase

MICROBIOLOGY-SGM, 153, 3295–3302.

Modification of Lactobacillus beta-glucuronidase activity by random mutagenesis

GENE, 389(2), 122–127.

The genomics of lactic acid bacteria

[Review of ]. TRENDS IN MICROBIOLOGY, 15(12), 546–553.

Analysis of treatment effects on the microbial ecology of the human intestine

FEMS MICROBIOLOGY ECOLOGY, 57(2), 239–250.

Characterization of the tre locus and analysis of trehalose cryoprotection in Lactobacillus acidophilus NCFM

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72(2), 1218–1225.

Comparative genomics and transcriptional analysis of prophages identified in the Genomes of Lactobacillus gasseri, Lactobacillus salivarius, and Lactobacillus casei

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72(5), 3130–3146.

Comparative genomics of the lactic acid bacteria

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 103(42), 15611–15616.

Engineered bacteriophage-defence systems in bioprocessing

[Review of ]. NATURE REVIEWS MICROBIOLOGY, 4(5), 395–404.

Global analysis of carbohydrate utilization by Lactobacillus acidophilus using cDNA microarrays

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 103(10), 3816–3821.

Transcriptional and functional analysis of oxalyl-coenzyme A (CoA) decarboxylase and formyl-CoA transferase genes from Lactobacillus acidophilus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72(3), 1891–1899.

Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 102(11), 3906–3912.

Functional analysis of putative adhesion factors in Lactobacillus acidophilus NCFM

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(12), 8344–8351.

Genetic analysis of two bile salt hydrolase activities in Lactobacillus acidophilus NCFM

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(8), 4925–4929.

Genomic Perspectives on Probiotic Lactic Acid Bacteria

Bioscience and Microflora, 24(2), 31–33.

Genomic features of lactic acid bacteria effecting bioprocessing and health

[Review of ]. FEMS MICROBIOLOGY REVIEWS, 29(3), 393–409.

Lactobacilli activate human dendritic cells that skew T cells toward T helper 1 polarization

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 102(8), 2880–2885.

Marker-free chromosomal integration of the manganese superoxide dismutase gene (sodA) from Streptococcus thermophilus into Lactobacillus gasseri

FEMS MICROBIOLOGY LETTERS, 246(1), 91–101.

Microarray analysis of a two-component regulatory system involved in acid resistance and proteolytic activity in Lactobacillus acidophilus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(10), 5794–5804.

Modulation of the microbial ecology of the human colon by probiotics, prebiotics and synbiotics to enhance human health: An overview of enabling science and potential applications

[Review of ]. FEMS MICROBIOLOGY ECOLOGY, 52(2), 145–152.

PathwayVoyager: pathway mapping using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database

BMC Genomics, 6.

Sequence analysis of the Lactobacillus plantarum bacteriophage Phi JL-1

GENE, 348, 45–54.

Antisense RNA expression strategies effective against Streptococcus thermophilus bacteriophages

Washington, DC: U.S. Patent and Trademark Office.

Antisense RNA targeting of primase interferes with bacteriophage replication in Streptococcus thermophilus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(3), 1735–1743.

Bacteriophage defense systems and strategies for lactic acid bacteria

[Review of ]. ADVANCES IN APPLIED MICROBIOLOGY, VOL 56, 56, 331-+.

DNA analysis of the genes encoding acidocin LF221 A and acidocin LF221 B, two bacteriocins produced by Lactobacillus gasseri LF221

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 63(6), 705–714.

Epidemic clone I-specific genetic markers in strains of Listeria monocytogenes serotype 4b from foods

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(7), 4158–4164.

Epidemic clone I-specific genetic markers in strains of Listeria monocytogenes serotype 4b from foods (vol 70, pg 4158, 2004)

Yildirim, S., Lin, W., Hitchins, A. D., Jaykus, L. A., Altermann, E., Klaenhammer, T. R., & Kathariou, S. (2004, December). APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol. 70, pp. 7581–7581.

Expression of a heterologous manganese superoxide dismutase gene in intestinal lactobacilli provides protection against hydrogen peroxide toxicity

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(8), 4702–4710.

Genetic markers unique to Listeria monocytogenes serotype 4b differentiate epidemic clone II (hot dog outbreak strains) from other lineages

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(4), 2383–2390.

Identification and inactivation of genetic loci involved with Lactobacillus acidophilus acid tolerance

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(9), 5315–5322.

Identification and phenotypic characterization of the cell-division protein CdpA

GENE, 342(1), 189–197.

Lactobacillus acidophilus nucleic acids encoding fructo-oligosaccharide utilization compounds and uses thereof

Washington, DC: U.S. Patent and Trademark Office.

The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 101(8), 2512–2517.

A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes

NUCLEIC ACIDS RESEARCH, 31(7), 1805–1812.