Works (188)

Updated: April 4th, 2024 17:38

2021 journal article

A double-blind, 377-subject randomized study identifies Ruminococcus, Coprococcus, Christensenella, and Collinsella as long-term potential key players in the modulation of the gut microbiome of lactose intolerant individuals by galacto-oligosaccharides

GUT MICROBES, 13(1).

By: M. Azcarate-Peril*, J. Roach*, A. Marsh*, W. Chey*, W. Sandborn*, A. Ritter, D. Savaiano*, T. Klaenhammer n

author keywords: Prebiotics; microbiome modulation; lactose intolerance; human lactase; Bifidobacterium; short chain fatty acids; galacto-oligosaccharides; GOS
MeSH headings : Actinobacteria / classification; Actinobacteria / genetics; Actinobacteria / isolation & purification; Clostridiales / classification; Clostridiales / genetics; Clostridiales / isolation & purification; Double-Blind Method; Gastrointestinal Microbiome / physiology; Humans; Lactobacillus / growth & development; Lactobacillus / metabolism; Lactose Intolerance / microbiology; Oligosaccharides / administration & dosage; Oligosaccharides / metabolism; Placebos / administration & dosage; Prebiotics / administration & dosage; RNA, Ribosomal, 16S / genetics
TL;DR: An in-depth analysis of the fecal microbiome of the 377 LI patients randomized to one of two GOS doses or placebo in a multi-center, double-blinded, placebo-controlled trial confirmed and expanded data on GOS microbiome modulation in LI individuals. (via Semantic Scholar)
Source: Web Of Science
Added: August 16, 2021

2021 journal article

In Vivo Transcriptome of Lactobacillus acidophilus and Colonization Impact on Murine Host Intestinal Gene Expression

MBIO, 12(1).

By: Y. Goh n, R. Barrangou n & T. Klaenhammer n

Ed(s): G. Huffnagle

author keywords: Lactobacillus; acidophilus; gut adaptation; in vivo gene expression; mouse colonization; probiotic
MeSH headings : Animals; Bacterial Adhesion; Bacterial Proteins / genetics; Gene Expression Profiling; Host Microbial Interactions / genetics; Immunity; Intestines / microbiology; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / physiology; Mice; Probiotics / administration & dosage; Sequence Analysis, RNA; Signal Transduction; Transcriptome
TL;DR: Metagenomic analysis of NCFM populations postcolonization demonstrated the genomic stability of L. acidophilus as a gut transient and further established its safety as a probiotic and biotherapeutic delivery platform. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: January 26, 2021

2019 article

Get Cultured: Eat Bacteria

ANNUAL REVIEW OF FOOD SCIENCE AND TECHNOLOGY, VOL 10, Vol. 10, pp. 1–20.

By: T. Klaenhammer n

author keywords: Klaenhammer; food science; microbiology; genomics; biotechnology; lactic acid bacteria; probiotics; prebiotics
MeSH headings : Awards and Prizes; Bacteria / genetics; Biological Transport; Biotechnology; Food Microbiology; Genome, Bacterial; Humans; Prebiotics; Probiotics
TL;DR: The Klaenhammer group at North Carolina State University pioneered genomic applications in food microbiology and beneficial lactic acid bacteria used as starter cultures and probiotics and as oral delivery vehicles for vaccines and biotherapeutics. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: April 22, 2019

2018 journal article

Deletion-based escape of CRISPR-Cas9 targeting in Lactobacillus gasseri

MICROBIOLOGY-SGM, 164(9), 1098–1111.

By: E. Stout n, R. Sanozky-Dawes n, Y. Goh n, A. Crawley n, T. Klaenhammer n & R. Barrangou n

author keywords: Lactobacillus; CRISPR; Cas; Cas9; interference; spacer
MeSH headings : CRISPR-Associated Protein 9 / metabolism; Clustered Regularly Interspaced Short Palindromic Repeats; Interspersed Repetitive Sequences; Lactobacillus gasseri / genetics; Mutation; Plasmids; Recombination, Genetic; Sequence Deletion; Transformation, Bacterial
TL;DR: It is shown that deletions of spacers within CRISPR arrays constitute a key escape mechanism to evadeCRISPR targeting, while preserving the functionality of the CRISpr-Cas system. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: December 31, 2018

2018 journal article

Nod2 is required for antigen-specific humoral responses against antigens orally delivered using a recombinant Lactobacillus vaccine platform

PLOS ONE, 13(5).

By: S. Bumgardner n, L. Zhang*, A. LaVoy*, B. Andre*, C. Frank*, A. Kajikawa*, T. Klaenhammer n, G. Dean*

MeSH headings : Administration, Oral; Animals; Antigens / administration & dosage; Caspase 1 / genetics; Caspase 1 / immunology; Genes, gag / genetics; HIV-1 / immunology; HIV-1 / pathogenicity; Humans; Immunity, Humoral / genetics; Immunity, Humoral / immunology; Inflammasomes / genetics; Inflammasomes / immunology; Interleukin-1beta / genetics; Interleukin-1beta / immunology; Lactobacillus acidophilus / immunology; Lipopolysaccharides / immunology; Lipopolysaccharides / metabolism; Macrophages / immunology; Macrophages / microbiology; Mice; Nod2 Signaling Adaptor Protein / genetics; Nod2 Signaling Adaptor Protein / immunology; Teichoic Acids / immunology; Teichoic Acids / metabolism; Toll-Like Receptor 2 / genetics; Toll-Like Receptor 2 / immunology; Vaccines / administration & dosage; Vaccines / immunology
TL;DR: It is demonstrated that NOD2 signaling is required for antigen-specific mucosal and systemic humoral responses to a NCFM-based mucosal vaccine platform with surface expression of human immunodeficiency virus type 1 (HIV-1) Gag or membrane proximal external region (MPER). (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2018 journal article

Phenotypic and genotypic diversity of Lactobacillus buchneri strains isolated from spoiled, fermented cucumber

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 280, 46–56.

By: K. Daughtry n, S. Johanningsmeier n, R. Sanozky-Dawes n, T. Klaenhammer n & R. Barrangou n

author keywords: Lactic acid bacteria; Pickled vegetables; Spoilage organisms; Lactobacillus buchneri; Strain diversity
MeSH headings : Acetic Acid / metabolism; Bioreactors; Cucumis sativus / microbiology; Fermentation; Genotype; Lactic Acid / metabolism; Lactobacillus / classification; Lactobacillus / genetics; Lactobacillus / isolation & purification; Lactobacillus / metabolism; Propylene Glycol / metabolism; RNA, Ribosomal, 16S / genetics
TL;DR: Describing isolates of L. buchneri purified from both industrial and experimental fermented cucumber after the onset of secondary fermentation provided the basis for further analysis of metabolic and genomic capabilities of this species to enable control of lactic acid degradation in fermented plant materials. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2017 journal article

An Extracellular Cell-Attached Pullulanase Confers Branched alpha-Glucan Utilization in Human Gut Lactobacillus acidophilus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 83(12).

author keywords: glycoside hydrolase family 13; human gut microbiota; starch; starch binding modules
MeSH headings : Amylopectin / chemistry; Amylopectin / metabolism; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Enzyme Stability; Gastrointestinal Tract / microbiology; Glucans / chemistry; Glucans / metabolism; Glycoside Hydrolases / chemistry; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Humans; Hydrolysis; Kinetics; Lactobacillus acidophilus / enzymology; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / metabolism; Substrate Specificity
TL;DR: The pivotal role of debranching enzymes in the breakdown of starchy branched α-glucan oligomers by human gut lactobacilli exemplified by Lactobacillus acidophilus NCFM, which is one of the best-characterized strains used as probiotics, is highlighted. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2017 article

CRISPR-Cas Technologies and Applications in Food Bacteria

ANNUAL REVIEW OF FOOD SCIENCE AND TECHNOLOGY, VOL 8, Vol. 8, pp. 413–437.

By: E. Stout n, T. Klaenhammer n & R. Barrangou n

author keywords: probiotics; cultures; strain-typing; phage resistance; plasmid vaccination; antimicrobial; genome editing
MeSH headings : Bacteria / genetics; Bacteria / immunology; Bacterial Vaccines; Bacteriophages / genetics; CRISPR-Cas Systems; Food Industry; Food Microbiology; Gene Editing; Genes, Bacterial; Interspersed Repetitive Sequences; Probiotics
TL;DR: The applications of CRISPR-Cas systems and tools in starter cultures and probiotics are introduced, encompassing strain-typing, phage resistance, plasmid vaccination, genome editing, and antimicrobial activity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2017 journal article

Deletion of Lipoteichoic Acid Synthase Impacts Expression of Genes Encoding Cell Surface Proteins in Lactobacillus acidophilus

Frontiers in Microbiology, 8.

By: K. Selle n, Y. Goh n, B. Johnson n, O. Sarah n, J. Andersen n, R. Barrangou n, T. Klaenhammer n

TL;DR: Results elucidate the impact of ltaS deletion on the transcriptome and extracellular proteins of L. acidophilus, suggesting roles of LTA in cell morphology and ion homeostasis as a structural component of the Gram positive cell wall. (via Semantic Scholar)
Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2017 journal article

Lactobacillus acidophilus Metabolizes Dietary Plant Glucosides and Externalizes Their Bioactive Phytochemicals

MBIO, 8(6).

By: M. Theilmann*, Y. Goh n, K. Nielsen*, T. Klaenhammer n, R. Barrangou n & M. Abou Hachem*

Ed(s): E. Martens & M. McFall-Ngai

author keywords: Lactobacillus; beta-glucoside; bioavailability; gut microbiota; phytochemical; polydatin; polyphenols; resveratrol; xenobiotic metabolism
MeSH headings : Biological Availability; Dietary Carbohydrates / metabolism; Gastrointestinal Microbiome / physiology; Glucosides / metabolism; Glucosides / pharmacology; Humans; Hydrolysis; Lactobacillus acidophilus / drug effects; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / growth & development; Lactobacillus acidophilus / metabolism; Phytochemicals / metabolism; Polyphenols / metabolism; Resveratrol; Stilbenes / metabolism
TL;DR: This work demonstrates the growth of Lactobacillus acidophilus on mono- and diglucosyl dietary plant glycosides (PGs) possessing small aromatic aglycones and highlights an important role of lactobacilli in the bioconversion of dietary PG and presents a framework from which to derive molecular insights into their metabolism by members of the human gut microbiota. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2017 journal article

The S-layer Associated Serine Protease Homolog PrtX Impacts Cell Surface-Mediated Microbe-Host Interactions of Lactobacillus acidophilus NCFM

FRONTIERS IN MICROBIOLOGY, 8.

By: B. Johnson n, S. O'Flaherty n, Y. Goh n, I. Carroll*, R. Barrangou n & T. Klaenhammer n

author keywords: serine protease; S-layer; S-layer associated proteins; Lactobacillus; probiotic; intestinal barrier integrity; mucin; fibronectin
TL;DR: The absence of PrtX within the exoproteome of a ΔprtX strain caused morphological changes, resulting in a pleiotropic increase of the organisms’ immunomodulatory properties and interactions with some intestinal epithelial cell components. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2016 journal article

AcmB Is an S-Layer-Associated beta-N-Acetylglucosaminidase and Functional Autolysin in Lactobacillus acidophilus NCFM

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(18), 5687–5697.

By: B. Johnson n & T. Klaenhammer n

MeSH headings : Acetylglucosaminidase / genetics; Acetylglucosaminidase / metabolism; Bacterial Adhesion; Bacteriolysis; Cell Division; Cell Wall / chemistry; Computational Biology; Gene Deletion; Genetic Complementation Test; Lactobacillus acidophilus / enzymology; Lactobacillus acidophilus / genetics; Membrane Glycoproteins / genetics; Membrane Glycoproteins / metabolism; N-Acetylmuramoyl-L-alanine Amidase / genetics; N-Acetylmuramoyl-L-alanine Amidase / metabolism; Peptidoglycan / analysis
TL;DR: It is demonstrated that multiple autolysins, responsible for breaking down the cell wall during cell division, are associated with the S-layer, the most widely used probiotic microbes incorporated in many dairy foods and dietary supplements. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Conserved S-Layer-Associated Proteins Revealed by Exoproteomic Survey of S-Layer-Forming Lactobacilli

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(1), 134–145.

By: B. Johnson n, J. Hymes n, R. Sanozky-Dawes n, E. Henriksen n, R. Barrangou n & T. Klaenhammer n

Ed(s): H. Nojiri

MeSH headings : Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Lactobacillus / chemistry; Lactobacillus / classification; Lactobacillus / genetics; Lactobacillus / metabolism; Lactobacillus acidophilus / chemistry; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / metabolism; Membrane Glycoproteins / chemistry; Membrane Glycoproteins / genetics; Membrane Glycoproteins / metabolism; Molecular Sequence Data; Phylogeny; Proteomics
TL;DR: Proteomic complexity and differences in the cell surface properties between S-layer- and non-S- layer-forming lactobacilli reveal the potential for SLAPs to mediate intimate probiotic interactions and signaling with the host intestinal mucosa. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2016 journal article

Development of a Transportation System Simulation Manual Framework From Theory to Practice

TRANSPORTATION RESEARCH RECORD, (2561), 45–52.

By: G. List n, M. Mashayekhi n, R. Bertini* & B. Nevers

TL;DR: A possible structure for a transportation system simulation manual that would cover the limitations and gaps in the existing literature is proposed and would consist of five volumes: concepts, model building, verification and validation, results analysis, and case studies and supplementary materials. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Differential proteome and cellular adhesion analyses of the probiotic bacterium Lactobacillus acidophilus NCFM grown on raffinose - an emerging prebiotic

PROTEOMICS, 16(9), 1361–1375.

By: H. Celebioglu*, M. Ejby*, A. Majumder*, C. Kobler*, Y. Goh n, K. Thorsen*, B. Schmidt*, S. O'Flaherty n ...

author keywords: Adhesion; HT-29 cells; Moonlighting proteins; Probiotics; Raffinose; Surface and whole cell proteomes
TL;DR: Insight at the molecular and cellular levels contributed to the understanding of the interplay of a synbiotic composed of NCFM and raffinose with the host. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Functional Analysis of an S-Layer-Associated Fibronectin-Binding Protein in Lactobacillus acidophilus NCFM

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(9), 2676–2685.

By: J. Hymes n, B. Johnson n, R. Barrangou n & T. Klaenhammer n

Ed(s): E. Dudley

MeSH headings : Bacterial Adhesion / physiology; Bacterial Proteins / chemistry; Bacterial Proteins / metabolism; Fibronectins / metabolism; Intestines / microbiology; Lactobacillus acidophilus / chemistry; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / metabolism; Membrane Glycoproteins / metabolism; Mutation; Phylogeny; Protein Binding
TL;DR: To investigate the functional role of LBA0191 (designated FbpB) in L. acidophilus NCFM, an fbpB-deficient strain was constructed and a mutant with a deletion of fBPB lost the ability to adhere to mucin and fibronectin in vitro. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2016 journal article

Genomic evolution of lactic acid bacteria: From single gene function to the pan-genome

Biotechnology of Lactic Acid Bacteria: Novel Applications, 2nd Edition, 32–54.

By: G. Douglas*, M. Azcarate-Peril* & T. Klaenhammer n

UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018

2016 journal article

Intracellular and Extracellular Expression of Bacillus thuringiensis Crystal Protein Cry5B in Lactococcus lactis for Use as an Anthelminthic

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(4), 1286–1294.

By: E. Durmaz n, Y. Hu*, R. Aroian* & T. Klaenhammer n

MeSH headings : Animals; Anthelmintics / metabolism; Anthelmintics / pharmacology; Bacillus thuringiensis Toxins; Bacterial Proteins / biosynthesis; Bacterial Proteins / genetics; Bacterial Proteins / pharmacology; Blotting, Western; Caenorhabditis elegans / drug effects; Cloning, Molecular; Endotoxins / biosynthesis; Endotoxins / genetics; Endotoxins / pharmacology; Gene Dosage; Gene Expression; Genetic Vectors; Hemolysin Proteins / biosynthesis; Hemolysin Proteins / genetics; Hemolysin Proteins / pharmacology; Lactococcus lactis / genetics; Lactococcus lactis / metabolism; Microbial Viability; Nisin / metabolism; Plasmids; Promoter Regions, Genetic; Protein Transport; Recombinant Proteins / biosynthesis; Recombinant Proteins / genetics; Recombinant Proteins / pharmacology; Survival Analysis; Transcriptional Activation / drug effects
TL;DR: Results indicate that active Cry5B proteins can be expressed intracellularly in and released extracellularly from L. lactis, showing potential for future use as an anthelminthic that could be delivered orally in a food-grade microbe. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2016 journal article

Multivalent Chromosomal Expression of the Clostridium botulinum Serotype A Neurotoxin Heavy-Chain Antigen and the Bacillus anthracis Protective Antigen in Lactobacillus acidophilus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(20), 6091–6101.

By: S. O'Flaherty n & T. Klaenhammer n

MeSH headings : Anthrax / microbiology; Anthrax / prevention & control; Bacillus anthracis / genetics; Bacillus anthracis / metabolism; Bacterial Vaccines / genetics; Bacterial Vaccines / metabolism; Botulinum Toxins, Type A / genetics; Botulinum Toxins, Type A / metabolism; Botulism / microbiology; Botulism / prevention & control; Chromosomes, Bacterial / genetics; Chromosomes, Bacterial / metabolism; Clostridium botulinum / genetics; Clostridium botulinum / metabolism; Gene Expression; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / metabolism
TL;DR: It is demonstrated that chromosomal integrative recombinant strains are promising vaccine delivery vehicles when targeted into high-expression chromosomal regions and eliminate the requirement for antibiotic selective maintenance of recombinant plasmids. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2016 review

Stuck in the Middle: Fibronectin-Binding Proteins in Gram-Positive Bacteria

[Review of ]. FRONTIERS IN MICROBIOLOGY, 7.

By: J. Hymes n & T. Klaenhammer n

author keywords: fibronectin; Lactobacillus; Acidophifus; lactobacilli; streptococci
TL;DR: The interaction between human fibronectin and bacterial adhesins is described by highlighting the FnBPs of Gram-positive pathogens and commensals by providing an overview of the occurrence and diversity of FnBBP with a focus on the model pathogenic organisms in which FnBPS are most characterized. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2015 journal article

CRISPR-based screening of genomic island excision events in bacteria

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 112(26), 8076–8081.

By: K. Selle n, T. Klaenhammer n & R. Barrangou n

author keywords: CRISPR; lactic acid bacteria; transposons; IS-elements; Cas9
MeSH headings : Clustered Regularly Interspaced Short Palindromic Repeats; Gene Deletion; Genome, Bacterial; Homeostasis; Homologous Recombination; Streptococcus thermophilus / genetics; Streptococcus thermophilus / growth & development
TL;DR: This study shows that CRISPR-Cas systems can be directed to target and delete genomic islands that are flanked by insertion-sequence elements and devoid of essential genes, and established that self-targeting CRISpr-cas systems may direct significant evolution of bacterial genomes on a population level, influencing genome homeostasis and remodeling. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2015 journal article

Expanding the biotechnology potential of lactobacilli through comparative genomics of 213 strains and associated genera

NATURE COMMUNICATIONS, 6.

By: Z. Sun*, H. Harris*, A. McCann*, C. Guo*, S. Argimon*, W. Zhang*, X. Yang*, I. Jeffery* ...

MeSH headings : Biotechnology; Genome, Bacterial; Lactobacillus / enzymology; Lactobacillus / genetics; Leuconostoc / genetics; Pediococcus / genetics; Phylogeny; Sequence Analysis, DNA
TL;DR: The genome sequences of 213 different Lactobacilli strains and associated genera are reported, and new insight is provided into phylogenomic organization and adaptive immunity elements in this bacteria family, presenting a robust phylogenomic framework of existing species and for classifying new species. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2015 article

Genetic Mechanisms of Prebiotic Oligosaccharide Metabolism in Probiotic Microbes

ANNUAL REVIEW OF FOOD SCIENCE AND TECHNOLOGY, VOL 6, Vol. 6, pp. 137–156.

By: Y. Goh n & T. Klaenhammer n

author keywords: Lactobacillus; Bifidobacterium; fructooligosaccharide; galactooligosaccharide; prebiotic; probiotic
MeSH headings : Bifidobacterium / metabolism; Lactobacillus / metabolism; Oligosaccharides / metabolism; Prebiotics; Probiotics
TL;DR: This review highlights recent findings on the genetic mechanisms involved in the utilization of prebiotic fructooligosaccharides, β-galactooligOSaccharide, human milk oligosac charides, and other prebiotics candidates by these probiotic microbes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Mucosal Immunogenicity of Genetically Modified Lactobacillus acidophilus Expressing an HIV-1 Epitope within the Surface Layer Protein

PLOS ONE, 10(10).

By: A. Kajikawa*, L. Zhang*, A. LaVoy*, S. Bumgardner n, T. Klaenhammer n & G. Dean*

MeSH headings : Adaptive Immunity; Animals; Antibodies, Bacterial / immunology; Antibody Formation / immunology; Antibody Specificity / immunology; Bacterial Proteins / genetics; Bacterial Proteins / immunology; Cytokines / metabolism; Epitopes / genetics; Epitopes / immunology; HIV-1 / genetics; HIV-1 / immunology; Humans; Immunity, Mucosal; Immunization; Immunoglobulin A / immunology; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / immunology; Mice; Models, Animal; Mucous Membrane / immunology; Mucous Membrane / microbiology; Mucous Membrane / virology; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / immunology
TL;DR: The constructed genetically modified Lactobacillus acidophilus strains expressing the membrane proximal external region (MPER) from human immunodeficiency virus type 1 (HIV-1) within the context of the major S-layer protein, SlpA, demonstrated the potential use of the LactOBacillus S- layer protein for development of oral vaccines targeting specific peptides. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2015 journal article

Occurrence and activity of a type II CRISPR-Cas system in Lactobacillus gasseri

MICROBIOLOGY-SGM, 161, 1752–1761.

By: R. Sanozky-Dawes n, K. Selle n, S. O'Flaherty n, T. Klaenhammer n & R. Barrangou n

MeSH headings : Base Sequence; CRISPR-Cas Systems / genetics; Clustered Regularly Interspaced Short Palindromic Repeats; Gene Order; Genome, Bacterial; Lactobacillus / genetics; Molecular Sequence Data; Plasmids / genetics
TL;DR: The distribution and function of a native type II-A CRISPR-Cas system in the commensal species L. gasseri is reported, which open avenues for applications for bacteriophage protection and genome modification in L.gasseri, and contribute to the fundamental understanding of CRISpr-Cas systems in bacteria. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2015 journal article

SIGNR3-dependent immune regulation by Lactobacillus acidophilus surface layer protein A in colitis

EMBO JOURNAL, 34(7), 881–895.

By: Y. Lightfoot*, K. Selle n, T. Yang*, Y. Goh n, B. Sahay*, M. Zadeh*, J. Owen*, N. Colliou* ...

author keywords: colitis; immune regulation; Lactobacillus acidophilus; SIGNR3; surface layer protein A
MeSH headings : Animals; Antigens, CD / genetics; Antigens, CD / immunology; Bacterial Proteins / genetics; Bacterial Proteins / immunology; Inflammatory Bowel Diseases / genetics; Inflammatory Bowel Diseases / immunology; Inflammatory Bowel Diseases / microbiology; Inflammatory Bowel Diseases / pathology; Intestinal Mucosa / immunology; Intestinal Mucosa / pathology; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / immunology; Lectins, C-Type / genetics; Lectins, C-Type / immunology; Lipopolysaccharides / genetics; Lipopolysaccharides / immunology; Mice; Mice, Knockout; Protein Binding / genetics; Protein Binding / immunology; Teichoic Acids / genetics; Teichoic Acids / immunology
TL;DR: This work presents critical insights into SlpA/SIGNR3‐induced responses that are integral to the potential development of novel biological therapies for autoinflammatory diseases, including IBD. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2015 article

Sortase-deficient lactobacilli: effect on immunomodulation and gut retention

Call, E. K., Goh, Y. J., Selle, K., Klaenhammer, T. R., & O'Flaherty, S. (2015, February). MICROBIOLOGY-SGM, Vol. 161, pp. 311–321.

By: E. Call n, Y. Goh n, K. Selle n, T. Klaenhammer n & S. O'Flaherty n

MeSH headings : Aminoacyltransferases / genetics; Aminoacyltransferases / immunology; Aminoacyltransferases / metabolism; Animals; Bacterial Adhesion; Bacterial Proteins / genetics; Bacterial Proteins / immunology; Bacterial Proteins / metabolism; Caco-2 Cells; Cysteine Endopeptidases / genetics; Cysteine Endopeptidases / immunology; Cysteine Endopeptidases / metabolism; Dendritic Cells / immunology; Dendritic Cells / microbiology; Gastrointestinal Tract / immunology; Gastrointestinal Tract / microbiology; Humans; Immunomodulation; Lactobacillus / enzymology; Lactobacillus / genetics; Lactobacillus / growth & development; Lactobacillus / physiology; Lactobacillus acidophilus / enzymology; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / growth & development; Lactobacillus acidophilus / physiology; Mice; Swine; Tumor Necrosis Factor-alpha / immunology
TL;DR: It is shown for the first time that sortase-dependent proteins contribute to gut retention of probiotic microbes in the gastrointestinal tract. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2014 article

Effects of genetic, processing, or product formulation changes on efficacy and safety of probiotics

ANNALS REPORTS, Vol. 1309, pp. 1–18.

By: M. Sanders, T. Klaenhammer n, A. Ouwehand*, B. Pot*, E. Johansen*, J. Heimbach, M. Marco*, J. Tennila* ...

author keywords: probiotic; genetics; gene expression; regulatory; safety; efficacy
MeSH headings : Genetic Variation; Humans; Patient Safety; Probiotics / adverse effects; Probiotics / standards; Probiotics / therapeutic use; Treatment Outcome
TL;DR: This review explores whether genetic or phenotypic changes, by accident or design, might affect the efficacy or safety of commercial probiotics, and highlights key issues important to determining the need to re‐confirm efficacy orSafety after strain improvement, process optimization, or product formulation changes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2014 review

Impact of genomics on the field of probiotic research: historical perspectives to modern paradigms

[Review of ]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 106(1), 141–156.

By: B. Johnson n & T. Klaenhammer n

author keywords: Probiotic; Lactic acid bacteria; Fermentation; Genomics; Lactobacilli; Bifidobacteria
MeSH headings : Bacteria / classification; Bacteria / genetics; Bacteria / isolation & purification; Bacteria / metabolism; Biomedical Research / history; Canthaxanthin / history; Genomics / history; History, 19th Century; History, 20th Century; History, 21st Century; Humans; Probiotics / chemistry; Probiotics / history
TL;DR: The history of the probiotic concept and the current standard of integrated genomic techniques to discern the complex, beneficial relationships between probiotic microbes and their hosts are explored. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2014 review

Insights into glycogen metabolism in Lactobacillus acidophilus: impact on carbohydrate metabolism, stress tolerance and gut retention

[Review of ]. MICROBIAL CELL FACTORIES, 13.

By: Y. Goh n & T. Klaenhammer n

author keywords: Lactobacilli; Glycogen; Probiotic; Raffinose; Gastrointestinal retention
MeSH headings : Adaptation, Physiological / drug effects; Animals; Carbohydrate Metabolism / drug effects; Carbon / pharmacology; Colony Count, Microbial; Gastrointestinal Tract / drug effects; Gastrointestinal Tract / microbiology; Glycogen / biosynthesis; Glycogen / metabolism; Humans; Lactobacillus acidophilus / drug effects; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / growth & development; Lactobacillus acidophilus / metabolism; Metabolic Networks and Pathways / drug effects; Metabolic Networks and Pathways / genetics; Mice; Models, Biological; Mutation / genetics; Operon / genetics; Prokaryotic Cells / drug effects; Prokaryotic Cells / metabolism; Raffinose / pharmacology; Species Specificity; Stress, Physiological / drug effects; Trehalose / pharmacology
TL;DR: The present in vivo studies established the significance of glycogen biosynthesis on the competitive retention of L. acidophilus in the mouse intestinal tract, demonstrating for the first time that the ability to synthesize intracellular glycogen contributes to gut fitness and retention among probiotic microorganisms. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Microbiology bacteria get vaccinated

Nature, 513(7517), 175–176.

By: R. Barrangou & T. Klaenhammer

Source: NC State University Libraries
Added: August 6, 2018

2014 journal article

Recent insight in alpha-glucan metabolism in probiotic bacteria

BIOLOGIA, 69(6), 713–721.

By: M. Moller*, Y. Goh n, A. Viborg*, J. Andersen*, T. Klaenhammer n, B. Svensson*, M. Abou Hachem*

author keywords: prebiotic; oligosaccharide uptake; ATP-binding cassette transport system; phosphotransferase system; transcriptional analysis; isomalto-oligosaccharide; malto-oligosaccharide
TL;DR: This review presents some aspects of α-glucan metabolism in probiotic bacteria and highlights vague issues that merit experimental effort, especially oligosaccharide uptake and the functionally unassigned enzymes, featuring in this important facet of glycan turnover by members of the gut microbiota. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Spontaneously Induced Prophages in Lactobacillus gasseri Contribute to Horizontal Gene Transfer

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 80(11), 3508–3517.

By: J. Baugher n, E. Durmaz n & T. Klaenhammer n

MeSH headings : Bacteriophages / genetics; Bacteriophages / isolation & purification; Bacteriophages / physiology; DNA, Viral / chemistry; DNA, Viral / genetics; Gene Transfer, Horizontal; Host Specificity; Lactobacillus / virology; Molecular Sequence Data; Plasmids; Prophages / genetics; Prophages / isolation & purification; Prophages / physiology; Sequence Analysis, DNA; Transduction, Genetic; Virus Activation
TL;DR: The common occurrence of multiple prophages in L. gasseri genomes, their propensity for spontaneous induction, and the high degree of homology among phages within multiple species of Lactobacillus suggest that temperate bacteriophages likely contribute to horizontal gene transfer (HGT) in commensal lactobacilli. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

A functional glycogen biosynthesis pathway in Lactobacillus acidophilus: expression and analysis of the glg operon

MOLECULAR MICROBIOLOGY, 89(6), 1187–1200.

By: Y. Goh n & T. Klaenhammer n

MeSH headings : 1,4-alpha-Glucan Branching Enzyme / genetics; 1,4-alpha-Glucan Branching Enzyme / metabolism; Bile / metabolism; Biosynthetic Pathways / genetics; Carbon / metabolism; Energy Metabolism; Gene Deletion; Gene Expression Regulation, Bacterial; Glucose / metabolism; Glycogen / biosynthesis; Glycogen Phosphorylase / genetics; Glycogen Phosphorylase / metabolism; Glycogen Synthase / genetics; Glycogen Synthase / metabolism; Humans; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / growth & development; Lactobacillus acidophilus / metabolism; Operon; Raffinose / metabolism; Trehalose / metabolism
TL;DR: The data indicate that glycogen metabolism is involved in growth maintenance, bile tolerance and complex carbohydrate utilization in L. acidophilus, a probiotic microbe in the human gastrointestinal environment. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2013 review

Genomic and phenotypic evidence for probiotic influences of Lactobacillus gasseri on human health

[Review of ]. FEMS MICROBIOLOGY REVIEWS, 37(6), 915–935.

By: K. Selle n & T. Klaenhammer n

author keywords: gastrointestinal tract; acidophilus complex; bacteriocin; lactic acid bacteria; autochthonous; microbiota
MeSH headings : Animals; Bacteriocins / metabolism; Female; Gastrointestinal Tract / microbiology; Gastrointestinal Tract / physiology; Genome, Bacterial; Health; Helicobacter Infections / diet therapy; Helicobacter pylori; Homeostasis; Humans; Hypersensitivity / prevention & control; Immune System / physiology; Lactobacillus / classification; Lactobacillus / genetics; Lactobacillus / physiology; Probiotics / therapeutic use; Vagina / microbiology; Vagina / physiology; Virus Diseases / diet therapy
TL;DR: The genomic and empirical evidence supporting use of L. gasseri in probiotic applications is substantiated by clinical trial data displaying maintenance of vaginal homeostasis, mitigation of Helicobacter pylori infection, and amelioration of diarrhea. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Identification of extracellular surface-layer associated proteins in Lactobacillus acidophilus NCFM

MICROBIOLOGY-SGM, 159, 2269–2282.

By: B. Johnson n, K. Selle n, S. O'Flaherty n, Y. Goh n & T. Klaenhammer n

MeSH headings : Animals; Cells, Cultured; Chromatography, Liquid; Dendritic Cells / drug effects; Lactobacillus acidophilus / chemistry; Lactobacillus acidophilus / immunology; Mass Spectrometry; Membrane Glycoproteins / analysis; Membrane Glycoproteins / immunology; Mice; Tumor Necrosis Factor-alpha / metabolism
TL;DR: P phenotypic characterization of the deletion mutant demonstrated that the SLAP LBA1029 contributes to a pro-inflammatory TNF-α response from murine DCs, and appears to impart important surface display features and immunological properties to microbes that are coated by S-layers. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Improving lactose digestion and symptoms of lactose intolerance with a novel galacto-oligosaccharide (RP-G28): a randomized, double-blind clinical trial

NUTRITION JOURNAL, 12.

By: D. Savaiano*, A. Ritter, T. Klaenhammer n, G. James*, A. Longcore, J. Chandler, W. Walker*, H. Foyt

author keywords: Lactose intolerance; Colonic adaptation; Galacto-oligosaccharides; GOS; Hydrogen breath test; RP-G28; Microbiome; Microflora
MeSH headings : Abdominal Pain / epidemiology; Abdominal Pain / etiology; Abdominal Pain / prevention & control; Adult; Colon / microbiology; Dairy Products / adverse effects; Digestion; Double-Blind Method; Female; Follow-Up Studies; Gastrointestinal Agents / administration & dosage; Gastrointestinal Agents / adverse effects; Gastrointestinal Agents / therapeutic use; Humans; Incidence; Intestinal Mucosa / microbiology; Lactase / deficiency; Lactose / metabolism; Lactose Intolerance / diet therapy; Lactose Intolerance / microbiology; Lactose Intolerance / physiopathology; Male; Oligosaccharides / administration & dosage; Oligosaccharides / adverse effects; Oligosaccharides / therapeutic use; Prebiotics / adverse effects; Prebiotics / analysis; Severity of Illness Index; Trisaccharides / administration & dosage; Trisaccharides / adverse effects; Trisaccharides / therapeutic use; United States / epidemiology
TL;DR: RP-G28 appears to be a potentially useful approach for improving lactose digestion and LI symptoms and the concurrent reduction in abdominal pain and improved overall tolerance could be a meaningful benefit to lactose intolerant individuals. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Probiotics, prebiotics, and the host microbiome: the science of translation

ANNALS REPORTS, 1306, 1–17.

By: B. Petschow, J. Dore*, P. Hibberd*, T. Dinan*, G. Reid*, M. Blaser*, P. Cani*, F. Degnan* ...

author keywords: microbiome; probiotics; prebiotics; intestinal microbiota; health disorders
MeSH headings : Cognition; Congresses as Topic; Depression / therapy; Diabetes Mellitus / therapy; Humans; Intestines / microbiology; Lactose Intolerance / therapy; Mental Health; Microbiota; Obesity / therapy; Prebiotics / microbiology; Probiotics / therapeutic use; Translational Research, Biomedical
TL;DR: A group of experts recently met to review the latest advances in microbiota/microbiome research and discuss the implications for development of probiotics and prebiotics, primarily as they relate to effects mediated via the intestine. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2013 review

Recent insight into oligosaccharide uptake and metabolism in probiotic bacteria

[Review of ]. BIOCATALYSIS AND BIOTRANSFORMATION, 31(4), 226–235.

By: M. Abou Hachem*, J. Andersen*, R. Barrangou n, M. Moller*, F. Fredslund*, A. Majumder*, M. Ejby*, S. Lahtinen* ...

author keywords: Prebiotic; oligosaccharide uptake; ATP-binding cassette transport system; phosphotransferase system; transcriptional analysis
TL;DR: It is demonstrated for the first time that non-naturally occurring tri- and tetra-saccharides are internalized and efficiently utilized by probiotic bacteria in some cases better than well-established natural prebiotics. (via Semantic Scholar)
UN Sustainable Development Goal Categories
10. Reduced Inequalities (OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2013 review

Relevance and application of sortase and sortase-dependent proteins in lactic acid bacteria

[Review of ]. FRONTIERS IN MICROBIOLOGY, 4.

By: E. Call & T. Klaenhammer*

author keywords: probiotic; sortase; sortase-dependent protein; LPXTG; lactic acid bacteria; lactobacillus; lactococcus
TL;DR: This review examines the collection of studies which evaluate sortase and SDPs in select species of dairy-associated and health promoting LAB and sheds light on their important roles in mediating nutrient acquisition through proteinase P as well as positive probiotic attributes. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Transcriptional analysis of oligosaccharide utilization by Bifidobacterium lactis Bl-04

BMC GENOMICS, 14(1).

By: J. Andersen*, R. Barrangou*, M. Abou Hachem*, S. Lahtinen*, Y. Goh n, B. Svensson*, T. Klaenhammer n

author keywords: Bifidobacterium lactis; Transcriptomics; ABC transporter; GPH transporter; Prebiotics; Glycoside hydrolase
MeSH headings : Bifidobacterium / drug effects; Bifidobacterium / genetics; Cell Membrane / drug effects; Cell Membrane / metabolism; Data Mining; Gene Expression Profiling; Genetic Variation / genetics; Genomics; Multigene Family / genetics; Oligosaccharides / pharmacology; Prebiotics; Transcription, Genetic / drug effects; Up-Regulation / drug effects
TL;DR: This study identified the differential gene expression for utilization of potential prebiotics highlighting the extensive capabilities of Bifidobacterium lactis Bl-04 to utilize oligosaccharides. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2012 journal article

Abating colon cancer polyposis by Lactobacillus acidophilus deficient in lipoteichoic acid

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109(26), 10462–10467.

By: K. Khazaie*, M. Zadeh*, M. Khan, P. Bere*, F. Gounari*, K. Dennis, N. Blatner, J. Owen*, T. Klaenhammer n, M. Mohamadzadeh

author keywords: dendritic cells; regulatory T cells; anti-inflammatory
MeSH headings : Adenomatous Polyposis Coli / immunology; Adenomatous Polyposis Coli / pathology; Animals; Lactobacillus acidophilus / genetics; Lipopolysaccharides / genetics; Mice; T-Lymphocytes, Regulatory / immunology; Teichoic Acids / genetics
TL;DR: It is reported that oral treatment with LTA-deficient NCK2025 normalizes innate and adaptive pathogenic immune responses and causes regression of established colonic polyps in a unique mouse model. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Characterization of the fecal microbiota using high-throughput sequencing reveals a stable microbial community during storage

PLoS One, 7(10).

By: I. Carroll*, T. Ringel-Kulka*, J. Siddle*, T. Klaenhammer n & Y. Ringel*

MeSH headings : Bacteria / classification; Bacteria / genetics; Bacteria / growth & development; Biodiversity; DNA, Bacterial / chemistry; DNA, Bacterial / genetics; Feces / microbiology; Freezing; Genetic Variation; Humans; Intestines / microbiology; Irritable Bowel Syndrome / microbiology; Metagenome / genetics; RNA, Ribosomal, 16S / genetics; Sequence Analysis, DNA / methods; Specimen Handling / methods; Time Factors
TL;DR: Fecal samples exposed to room or deep freezing temperatures for up to twenty four hours and six months, respectively, exhibit a microbial composition and diversity that shares more identity with its host of origin than any other sample. (via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018

2012 journal article

Construction and Immunological Evaluation of Dual Cell Surface Display of HIV-1 Gag and Salmonella enterica Serovar Typhimurium FliC in Lactobacillus acidophilus for Vaccine Delivery

CLINICAL AND VACCINE IMMUNOLOGY, 19(9), 1374–1381.

By: A. Kajikawa*, L. Zhang*, J. Long n, S. Nordone n, L. Stoeker n, A. LaVoy*, S. Bumgardner n, T. Klaenhammer n, G. Dean*

MeSH headings : AIDS Vaccines / administration & dosage; AIDS Vaccines / genetics; AIDS Vaccines / immunology; Administration, Oral; Animals; Bacterial Vaccines / administration & dosage; Bacterial Vaccines / genetics; Bacterial Vaccines / immunology; Cell Surface Display Techniques / methods; Cells, Cultured; Dendritic Cells / immunology; Female; Flagellin / genetics; Flagellin / immunology; HIV-1 / genetics; HIV-1 / immunology; Humans; Intestinal Mucosa / immunology; Lactobacillus acidophilus / genetics; Leukocytes, Mononuclear / immunology; Mice; Mice, Inbred BALB C; Salmonella typhimurium / genetics; Salmonella typhimurium / immunology; Vaccines, Synthetic / administration & dosage; Vaccines, Synthetic / genetics; Vaccines, Synthetic / immunology; gag Gene Products, Human Immunodeficiency Virus / genetics; gag Gene Products, Human Immunodeficiency Virus / immunology
TL;DR: Results suggested that the Gag-displaying L. acidophilus elicited specific immune responses and the coexistence of FliC conferred an adjuvant effect on local IgA production. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Influence of Exposure Time on Gene Expression by Human Intestinal Epithelial Cells Exposed to Lactobacillus acidophilus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 78(14), 5028–5032.

By: S. O'Flaherty n & T. Klaenhammer n

MeSH headings : Caco-2 Cells; Epithelial Cells / immunology; Epithelial Cells / metabolism; Epithelial Cells / microbiology; Flagellin / metabolism; Flagellin / pharmacology; Gene Expression Profiling; Gene Expression Regulation; Humans; Intestinal Mucosa / metabolism; Intestines / cytology; Intestines / immunology; Intestines / microbiology; Lactobacillus acidophilus / immunology; Molecular Sequence Data; NF-kappa B / genetics; NF-kappa B / metabolism; Probiotics / pharmacology; Recombinant Proteins / metabolism; Recombinant Proteins / pharmacology; Time Factors
TL;DR: Analysis of global temporal gene expression by human intestinal cells when exposed to Lactobacillus acidophilus revealed induction of immune-related pathways and NF-κB target genes after a 1-h exposure, compared to a 4- or 8-H exposure. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Raffinose family oligosaccharide utilisation by probiotic bacteria: insight into substrate recognition, molecular architecture and diversity of GH36 alpha-galactosidases

BIOCATALYSIS AND BIOTRANSFORMATION, 30(3), 316–325.

By: M. Abou Hachem*, F. Fredslund*, J. Andersen*, R. Larsen*, A. Majumder*, M. Ejby*, G. Van Zanten*, S. Lahtinen* ...

author keywords: prebiotic; probiotic; tetrameric alpha-galactosidases; raffinose; clan GH-D
TL;DR: The structure of the GH36 homotetrameric α-galactosidase from L. acidophilus NCFM (LaMel36A) was determined, which explains the preference of tetrameric GH36 enzymes for RFO and their lack of activity on polymeric galacto(gluco)mannan. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2012 journal article

The impact of probiotics and prebiotics on the immune system

NATURE REVIEWS IMMUNOLOGY, 12(10), 728–734.

By: T. Klaenhammer n, M. Kleerebezem, M. Kopp & M. Rescigno

MeSH headings : Animals; Humans; Immune System; Immunomodulation; Prebiotics; Probiotics
TL;DR: Four experts are asked to discuss the future application of probiotics as a disease therapy and provide their thoughts on the growth and function of beneficial intestinal microorganisms. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Transcriptional Analysis of Prebiotic Uptake and Catabolism by Lactobacillus acidophilus NCFM

PLOS ONE, 7(9).

By: J. Andersen n, R. Barrangou*, M. Abou Hachem*, S. Lahtinen*, Y. Goh n, B. Svensson*, T. Klaenhammer n

Ed(s): C. Gibas

MeSH headings : ATP-Binding Cassette Transporters / metabolism; Cellobiose / pharmacology; Gene Expression Regulation, Bacterial / drug effects; Glucans / pharmacology; Isomaltose / analogs & derivatives; Isomaltose / pharmacology; Lactobacillus acidophilus / drug effects; Lactobacillus acidophilus / enzymology; Lactobacillus acidophilus / metabolism; Oligosaccharides / pharmacology; Phosphoenolpyruvate Sugar Phosphotransferase System / metabolism; Prebiotics; Raffinose / pharmacology; Sugar Alcohols / pharmacology
TL;DR: Differential transcriptomics and functional genomics were used to identify genes in Lactobacillus acidophilus NCFM involved in the uptake and catabolism of 11 potential prebiotic compounds consisting of α- and β- linked galactosides and glucosides, highlighting the broad oligosaccharide metabolic repertoire of L. acidophillus NCFM. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2011 article

A rendezvous with our microbes

Gordon, J. I., & Klaenhammer, T. R. (2011, March 15). PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol. 108, pp. 4513–4515.

By: J. Gordon & T. Klaenhammer n

MeSH headings : Bacteria; Biological Evolution; Humans; Microbiology / trends; Symbiosis
TL;DR: On November 2–3, 2009 an international group of scientists representing multiple disciplines gathered to consider the current state of the authors' understanding of the symbiotic and beneficial relationships between microbes and humans and to define the challenges, gaps in knowledge, and opportunities that this exciting field of study now offers. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Assessment of Lactobacillus gasseri as a Candidate Oral Vaccine Vector

CLINICAL AND VACCINE IMMUNOLOGY, 18(11), 1834–1844.

By: L. Stoeker n, S. Nordone n, S. Gunderson n, L. Zhang n, A. Kajikawa n, A. LaVoy n, M. Miller n, T. Klaenhammer n, G. Dean n

MeSH headings : Adjuvants, Immunologic / genetics; Administration, Oral; Animals; B-Lymphocytes / immunology; Bacterial Vaccines / administration & dosage; Bacterial Vaccines / genetics; Bacterial Vaccines / immunology; Blood / immunology; Colon / immunology; Cytokines / metabolism; Dendritic Cells / immunology; Dendritic Cells / microbiology; Drug Carriers / administration & dosage; Female; Flagellin / genetics; Genetic Vectors; Humans; Intestinal Mucosa / immunology; Lactobacillus / genetics; Lactobacillus / immunology; Mice; Mice, Inbred BALB C; Plasmids; Toll-Like Receptor 5 / immunology
TL;DR: In vivo studies established that treatment with L. gasseri led to a diversification of B-cell populations in the lamina propria of the murine colon and a significant decrease in the percentage of FoxP3+ colonic lymphocytes, and in vivo data clarify the interaction of L. Gasseri with the host immune system and support further investigation of the in vivo immunogenicity of the vector. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Directed Chromosomal Integration and Expression of the Reporter Gene gusA3 in Lactobacillus acidophilus NCFM

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 77(20), 7365–7371.

By: G. Douglas n & T. Klaenhammer n

MeSH headings : Artificial Gene Fusion / methods; Chromosomes, Bacterial; Gene Expression Profiling; Genes, Reporter; Genetic Vectors; Genetics, Microbial / methods; Glucuronidase / genetics; Glucuronidase / metabolism; Humans; Lactobacillus acidophilus / genetics; Mutagenesis, Insertional / methods; Plasmids; Probiotics; Recombination, Genetic
TL;DR: The differences in expression levels between integration locations highlights the importance of rational targeting with gene cassettes intended for chromosomal expression. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Dissimilar Properties of Two Recombinant Lactobacillus acidophilus Strains Displaying Salmonella FliC with Different Anchoring Motifs

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 77(18), 6587–6596.

By: A. Kajikawa n, S. Nordone, L. Zhang*, L. Stoeker*, A. LaVoy*, T. Klaenhammer n, G. Dean*

MeSH headings : Acids / metabolism; Antigens, Bacterial / genetics; Antigens, Bacterial / metabolism; Cells, Cultured; Cytokines / metabolism; Dendritic Cells / immunology; Dendritic Cells / microbiology; Drug Carriers; Drug Stability; Flagellin / genetics; Flagellin / metabolism; Genetic Vectors; Humans; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / metabolism; Peptide Hydrolases / metabolism; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Salmonella Vaccines / genetics; Salmonella Vaccines / immunology; Vaccines, Synthetic / genetics; Vaccines, Synthetic / immunology
TL;DR: Two recombinant L. acidophilus derivatives displaying Salmonella flagellin were constructed using different anchor motifs, and the surface-associated antigen was highly sensitive to simulated gastric and small intestinal juices. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Group-specific comparison of four lactobacilli isolated from human sources using differential blast analysis

GENES AND NUTRITION, 6(3), 319–340.

By: E. Altermann n & T. Klaenhammer n

author keywords: In silico analysis; PAU; Adhesion; Stress response; Bacteriophage
TL;DR: A comparative genomic analysis of four completely sequenced Lactobacillus strains, isolated from the human gastrointestinal tract, versus 25 lactic acid bacterial genomes present in the public database at the time of analysis. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2011 review

Invited review: Application of omics tools to understanding probiotic functionality

[Review of ]. JOURNAL OF DAIRY SCIENCE, 94(10), 4753–4765.

By: J. Baugher n & T. Klaenhammer n

author keywords: probiotics; omics; function; mechanism
MeSH headings : Bacterial Physiological Phenomena; Computational Biology; Humans; Intestines / microbiology; Probiotics / metabolism
TL;DR: The ability to examine fully sequenced and annotated genomes has greatly accelerated the application of genetic approaches to elucidate many important functional roles of probiotic microbes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
10. Reduced Inequalities (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2011 personal communication

Probiotic and prebiotic claims in Europe: seeking a clear roadmap

Guarner, F., Sanders, M. E., Gibson, G., Klaenhammer, T., Cabana, M., Scott, K., … Hill, C. (2011, December).

By: F. Guarner, M. Sanders, G. Gibson*, T. Klaenhammer n, M. Cabana*, K. Scott*, G. Reid*, N. Delzenne*, G. Fahey*, C. Hill*

MeSH headings : Europe; Prebiotics; Probiotics
TL;DR: The scientists and clinical investigators belonging to the Board of Directors of the International Scientific Association for Probiotics and Prebiotics (ISAPP) are concerned that claims supported by solid scientific evidence are also being rejected, and there is a lack of clarity regarding the criteria – from study design through wording of the claim – for a dossier suitable for a positive regulatory opinion. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Regulation of induced colonic inflammation by Lactobacillus acidophilus deficient in lipoteichoic acid

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 108, 4623–4630.

By: M. Mohamadzadeh*, E. Pfeiler n, J. Brown*, M. Zadeh, M. Gramarossa*, E. Managlia*, P. Bere*, B. Sarraj* ...

author keywords: antiinflammatory; lactobacilli; Toll-like receptor 2; innate immunity
MeSH headings : Animals; Autoimmunity / immunology; CD4-Positive T-Lymphocytes / immunology; Colitis / chemically induced; Colitis / immunology; Colitis / microbiology; DNA Primers / genetics; Dextran Sulfate / toxicity; Flow Cytometry; Fluorescent Antibody Technique; Gene Deletion; Gene Expression Regulation / immunology; Homeodomain Proteins / genetics; Interleukin-10 / genetics; Lactobacillus acidophilus / metabolism; Lipopolysaccharides / deficiency; Mice; Mice, Inbred C57BL; Mice, Knockout; Myeloid Differentiation Factor 88 / genetics; Polymerase Chain Reaction; Teichoic Acids; Transferases (Other Substituted Phosphate Groups) / genetics
TL;DR: Directed alteration of cell surface components of L. acidophilus NCFM establishes a potential strategy for the treatment of inflammatory intestinal disorders and significantly mitigated dextran sulfate sodium and CD4+CD45RBhighT cell-induced colitis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2011 article

The Impact of Omic Technologies on the Study of Food Microbes

ANNUAL REVIEW OF FOOD SCIENCE AND TECHNOLOGY, VOL 2, Vol. 2, pp. 353–371.

By: S. O'Flaherty n & T. Klaenhammer n

author keywords: sequencing; genome; probiotic; pathogen; microbiome; transcriptome
MeSH headings : Animals; Bioengineering; Food Microbiology / methods; Foodborne Diseases / prevention & control; Gene Expression Profiling; Genomics / methods; Gram-Negative Bacteria / classification; Gram-Negative Bacteria / genetics; Gram-Negative Bacteria / isolation & purification; Gram-Negative Bacteria / metabolism; Gram-Positive Bacteria / classification; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / isolation & purification; Gram-Positive Bacteria / metabolism; High-Throughput Nucleotide Sequencing; Humans; Metagenomics / methods; Molecular Typing; Proteomics / methods; Transcriptome
TL;DR: This review outlines advances in omic technologies and how these have impacted food microbiology through providing examples of recently published landmark work. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Transcriptional and functional analysis of galactooligosaccharide uptake by lacS in Lactobacillus acidophilus

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 108(43), 17785–17790.

By: J. Andersen n, R. Barrangou*, M. Abou Hachem*, S. Lahtinen, Y. Goh n, B. Svensson*, T. Klaenhammer n

author keywords: lactose permease; catabolite repression element
MeSH headings : DNA, Complementary / genetics; Gene Expression Regulation, Bacterial / physiology; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / metabolism; Membrane Transport Proteins / metabolism; Microarray Analysis; Oligonucleotide Array Sequence Analysis; Oligosaccharides / pharmacokinetics; Prebiotics; Reverse Transcriptase Polymerase Chain Reaction; beta-Galactosidase / genetics; beta-Galactosidase / metabolism
TL;DR: P phenotypic confirmation of the functional LacS permease not only for the utilization of lactose and GOS but also lactitol is confirmed, suggesting a prominent role of LacS in the metabolism of a broad range of prebiotic β-galactosides, known to selectively modulate the beneficial gut microbiota. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2010 journal article

Construction of vectors for inducible and constitutive gene expression in Lactobacillus

Microbial Biotechnology, 4(3), 357–367.

By: T. Duong n, M. Miller n, R. Barrangou n, M. Azcarate-Peril n & T. Klaenhammer n

MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Gene Expression; Gene Expression Regulation, Bacterial; Genetic Vectors / genetics; Genetic Vectors / metabolism; Lactobacillus / genetics; Lactobacillus / metabolism; Operon; Plasmids / genetics; Plasmids / metabolism
TL;DR: Development of these expression vectors could support several novel applications, including the expression of enzymes, proteins, vaccines and biotherapeutics by intestinal lactobacilli. (via Semantic Scholar)
Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2010 journal article

Functional Roles of Aggregation-Promoting-Like Factor in Stress Tolerance and Adherence of Lactobacillus acidophilus NCFM

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76(15), 5005–5012.

By: Y. Goh n & T. Klaenhammer n

MeSH headings : Adhesins, Bacterial / chemistry; Adhesins, Bacterial / genetics; Adhesins, Bacterial / metabolism; Anti-Bacterial Agents / toxicity; Bacterial Adhesion; Bile; Detergents / toxicity; Epithelial Cells / microbiology; Fibronectins / metabolism; Gastric Juice; Gene Deletion; Gene Expression Profiling; Lactobacillus acidophilus / drug effects; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / physiology; Microbial Viability / drug effects; Molecular Weight; Mucins / metabolism; Phylogeny; Protein Binding; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Amino Acid; Sodium Dodecyl Sulfate / toxicity
TL;DR: R reverse transcriptase-quantitative PCR analysis revealed that the apf gene was highly induced during the stationary phase compared to that during the logarithmic phase, suggesting that the Apf-like proteins may contribute to the survival of L. acidophilus during transit through the digestive tract and, potentially, participate in the interactions with the host intestinal mucosa. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Functional and phenotypic characterization of a protein from Lactobacillus acidophilus involved in cell morphology, stress tolerance and adherence to intestinal cells

MICROBIOLOGY-SGM, 156, 3360–3367.

By: . Sarah J. O'Flaherty & T. Klaenhammer n

MeSH headings : Bacterial Adhesion; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Caco-2 Cells; Cell Wall / metabolism; Gene Deletion; Humans; Intestines / microbiology; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / growth & development; Lactobacillus acidophilus / metabolism; Membrane Proteins / genetics; Membrane Proteins / metabolism; Mutation; Stress, Physiological
TL;DR: Results indicate a role for LBA649 in stress tolerance, cell wall division and adherence to Caco-2 cells in L. acidophilus NCFM. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2010 article

Genomic Evolution of Domesticated Microorganisms

ANNUAL REVIEW OF FOOD SCIENCE AND TECHNOLOGY, VOL 1, Vol. 1, pp. 397–414.

By: G. Douglas n & T. Klaenhammer n

author keywords: lactic acid bacteria; fermentation; food; adaptation; mutation
MeSH headings : Adaptation, Biological; Evolution, Molecular; Fermentation; Food Handling; Food Microbiology / methods; Fungi / genetics; Fungi / metabolism; Genome, Bacterial; Humans; Lactobacillales / genetics; Lactobacillales / metabolism; Microbial Viability; Selection, Genetic
TL;DR: This review highlights the evolutionary traits influenced by the domestication process as these microbes adapted to nutrient-rich foods developed by humans. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Plasmid Transduction Using Bacteriophage Phi adh for Expression of CC Chemokines by Lactobacillus gasseri ADH

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76(12), 3878–3885.

By: L. Damelin, D. Mavri-Damelin*, T. Klaenhammer n & C. Tiemessen

MeSH headings : Bacteriophages / genetics; Chemokines, CC / biosynthesis; Chemokines, CC / genetics; Chemokines, CC / metabolism; Genetic Engineering; Humans; Lactobacillus / genetics; Lactobacillus / metabolism; Plasmids; Recombinant Proteins / biosynthesis; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Transduction, Genetic
TL;DR: The findings of this study lend support to the potential use of transduction in vaginal Lactobacillus species as a novel strategy for the prevention of HIV infection across mucosal membranes. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Targeted expression of anthrax protective antigen by Lactobacillus gasseri as an anthrax vaccine

FUTURE MICROBIOLOGY, 5(8), 1289–1296.

By: M. Mohamadzadeh*, E. Durmaz n, M. Zadeh, K. Pakanati*, M. Gramarossa*, V. Cohran*, T. Klaenhammer n

author keywords: anthrax vaccine; dendritic cell; high copy expression; Lactobacillus gasseri; oral vaccine delivery; probiotic
MeSH headings : Administration, Oral; Animals; Anthrax / prevention & control; Anthrax Vaccines / administration & dosage; Anthrax Vaccines / genetics; Anthrax Vaccines / immunology; Antibodies, Bacterial / blood; Antibodies, Neutralizing / blood; Antigens, Bacterial / genetics; Antigens, Bacterial / immunology; Antitoxins / blood; Bacterial Toxins / genetics; Bacterial Toxins / immunology; Genetic Vectors; Lactobacillus / genetics; Lactobacillus / immunology; Mice; Plasmids; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / immunology; Survival Analysis; T-Lymphocytes / immunology
TL;DR: Improvement of this improved expression vaccine strategy reduced the number of inoculations and length of the boosting period, leading to animal protection via efficacious bacterial adjuvanticity and safe oral delivery of this vaccine to mucosal immune cells, including dendritic cells. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2010 article

The role and potential of probiotic bacteria in the gut, and the communication between gut microflora and gut/host

O'Flaherty, S., & Klaenhammer, T. R. (2010, April). INTERNATIONAL DAIRY JOURNAL, Vol. 20, pp. 262–268.

By: S. O'Flaherty n & T. Klaenhammer n

TL;DR: This review outlines the recent important advances in this exciting area of research, which has led to a greater understanding of the critical interface between gut microbiota, probiotic bacteria and the host. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2009 patent

Compositions comprising promoter sequences and methods of use

By: R. Barrangou, A. Azcarate-Peril, E. Altermann, T. Duong & T. Klaenhammer

Source: NC State University Libraries
Added: August 6, 2018

2009 journal article

Dendritic cell targeting of Bacillus anthracis protective antigen expressed by Lactobacillus acidophilus protects mice from lethal challenge

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 106(11), 4331–4336.

By: M. Mohamadzadeh*, T. Duong n, S. Sandwick*, T. Hoover* & T. Klaenhammer n

author keywords: anthrax; lactic acid bacteria; mucosal immunity; oral; vaccine
MeSH headings : Administration, Oral; Animals; Anthrax / prevention & control; Anthrax Vaccines / administration & dosage; Anthrax Vaccines / immunology; Antibody Formation; Antigen Presentation; Antigens, Bacterial / administration & dosage; Antigens, Bacterial / genetics; Antigens, Bacterial / therapeutic use; Bacillus anthracis / immunology; Dendritic Cells / immunology; Immunity; Lactobacillus acidophilus / genetics; Mice
TL;DR: Development of this strategy for oral delivery of DC-targeted antigens provides a safe and protective vaccine via a bacterial adjuvant that may potentiate mucosal immune responses against deadly pathogens. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Development and Application of a upp-Based Counterselective Gene Replacement System for the Study of the S-Layer Protein SlpX of Lactobacillus acidophilus NCFM

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(10), 3093–3105.

By: Y. Goh n, M. Azcarate-Peril n, S. O'Flaherty n, E. Durmaz n, F. Valence*, J. Jardin*, S. Lortal*, T. Klaenhammer n

MeSH headings : Anti-Bacterial Agents / pharmacology; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Drug Resistance, Bacterial; Fluorouracil / pharmacology; Gene Deletion; Genetic Complementation Test; Lactobacillus acidophilus / genetics; Membrane Glycoproteins / genetics; Molecular Sequence Data; Mutagenesis, Insertional / methods; Pentosyltransferases / genetics; Pentosyltransferases / metabolism; Plasmids; Recombination, Genetic; Selection, Genetic
TL;DR: Use of the upp-encoded uracil phosphoribosyltransferase (UPRTase) of L. acidophilus NCFM was explored as a counterselection marker to positively select for recombinants that have resolved from chromosomal integration of pORI-based plasmids. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2009 review

Genome-scale analyses of health-promoting bacteria: probiogenomics

[Review of ]. NATURE REVIEWS MICROBIOLOGY, 7(1), 61–U77.

MeSH headings : Bifidobacterium / genetics; Bifidobacterium / metabolism; Genomics; Humans; Lactobacillus / genetics; Lactobacillus / metabolism; Probiotics
TL;DR: The application of probiogenomics in the elucidation of the molecular basis of probiosis is discussed using the well-recognized model probiotic bacteria genera Bifidobacterium and Lactobacillus as examples. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2009 review

Genomic features of Lactobacillus species

[Review of ]. Frontiers in Bioscience, 14, 1362–1386.

By: Y. Goh & T. Klaenhammer

Source: NC State University Libraries
Added: August 6, 2018

2009 review

Genomics of lactic acid bacteria

[Review of ]. FEMS MICROBIOLOGY LETTERS, 292(1), 1–6.

By: J. Schroeter n & T. Klaenhammer n

author keywords: lactic acid bacteria; genomics; fermentation; probiotics
MeSH headings : Evolution, Molecular; Genome, Bacterial; Genomics; Lactobacillaceae / genetics; Lactobacillaceae / physiology
TL;DR: Although a general trend of genome reduction was observed, certain niche-specific genes appear to be recently acquired and appear on plasmids or adjacent to prophages. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2009 article

Immunogenicity of Lactobacillus gasseri-FliC as an oral mucosal vaccine adjuvant for HIV

RETROVIROLOGY, Vol. 6.

By: S. Gunderson n, S. Nordone n, A. LaVoy n, L. Zhang n, T. Klaenhammer n & G. Dean n

TL;DR: The immunogenicity of a novel Lactobacillus gasseri mucosal vaccine vector for use in oral delivery of HIV antigens is investigated. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2009 patent

Lactobacillus acidophillus nucleic acid sequences encoding cell surface protein homologues and uses therefore

By: T. Klaenhammer, W. Russell, E. Altermann & B. Buck

Source: NC State University Libraries
Added: August 6, 2018

2009 patent

Lactobacillus acidophilus nucleic acid sequences encoding stress-related proteins and uses therefor

By: T. Klaenhammer, E. Altermann, M. Azcarate-Peril, O. McAuliffe & W. Russell

Source: NC State University Libraries
Added: August 6, 2018

2009 patent

Nucleic acid sequences encoding two-component sensing and regulatory proteins, antimicrobial proteins and uses therefor

By: T. Klaenhammer, W. Russell, E. Altermann & A. Azcarate-Peril

Source: NC State University Libraries
Added: August 6, 2018

2009 journal article

Probiotics to minimize the disruption of faecal microbiota in healthy subjects undergoing antibiotic therapy

JOURNAL OF MEDICAL MICROBIOLOGY, 58(5), 663–670.

By: A. Engelbrektson*, J. Korzenik*, A. Pittler*, M. Sanders, T. Klaenhammer n, G. Leyer, C. Kitts*

MeSH headings : Amoxicillin / therapeutic use; Anti-Bacterial Agents / pharmacology; Bacteroides / drug effects; Bacteroides / genetics; Bifidobacterium / drug effects; Bifidobacterium / genetics; Clavulanic Acid / therapeutic use; Clostridium / drug effects; Clostridium / genetics; Conserved Sequence; DNA Primers; DNA, Bacterial / genetics; Enterobacteriaceae / drug effects; Enterobacteriaceae / genetics; Eubacterium / drug effects; Eubacterium / genetics; Feces / microbiology; Humans; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Probiotics / therapeutic use
TL;DR: This study indicates this mixture of probiotics promotes a more rapid return to pre-antibiotic baseline faecal bacterial microbiota. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Role of Transporter Proteins in Bile Tolerance of Lactobacillus acidophilus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75(18), 6013–6016.

By: E. Pfeiler n & T. Klaenhammer n

MeSH headings : Anti-Bacterial Agents / metabolism; Anti-Bacterial Agents / pharmacology; Bile / chemistry; Bile / metabolism; Ciprofloxacin / metabolism; Ciprofloxacin / pharmacology; Detergents / metabolism; Detergents / pharmacology; Gene Deletion; Genes, Bacterial; Lactobacillus acidophilus / drug effects; Lactobacillus acidophilus / physiology; Membrane Transport Proteins / genetics; Membrane Transport Proteins / metabolism; Microbial Viability; Taurocholic Acid / metabolism; Taurocholic Acid / pharmacology
TL;DR: These strains showed unique patterns of sensitivity to a variety of inhibitory compounds, as well as differential accumulations of ciprofloxacin and taurocholate. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Role of autoinducer-2 on the adhesion ability of Lactobacillus acidophilus

JOURNAL OF APPLIED MICROBIOLOGY, 107(1), 269–279.

By: B. Buck n, M. Azcarate-Peril n & T. Klaenhammer n

author keywords: autoinducer-2; Caco-2; microarray; probiotic
MeSH headings : Bacterial Adhesion / genetics; Bacterial Proteins / genetics; Caco-2 Cells / microbiology; Carbon-Sulfur Lyases / genetics; DNA, Bacterial / genetics; Gene Expression Regulation, Bacterial; Homoserine / analogs & derivatives; Homoserine / genetics; Homoserine / metabolism; Humans; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / metabolism; Lactones / metabolism; Microarray Analysis; Mutation; Phylogeny; Quorum Sensing / genetics; RNA, Bacterial / genetics
TL;DR: Investigating whether or not the quorum‐sensing signalling molecule, autoinducer (AI)‐2, was produced by Lactobacillus acidophilus and affected adherence to intestinal epithelial cells found it likely promotes retention in the gastrointestinal tract and communication with the immune system. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Temporal gene expression and probiotic attributes of Lactobacillus acidophilus during growth in milk

JOURNAL OF DAIRY SCIENCE, 92(3), 870–886.

By: M. Azcarate-Peril n, R. Tallon n & T. Klaenhammer n

author keywords: Lactobacillus acidophilus; probiotic; microarray analysis; dairy
MeSH headings : Animals; Bacterial Adhesion / genetics; Caco-2 Cells; Carbohydrate Metabolism / genetics; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Humans; Hydrogen-Ion Concentration; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / growth & development; Lactobacillus acidophilus / metabolism; Milk / microbiology; Mucins / metabolism; Mutation; Peptide Hydrolases / genetics; Probiotics; Quorum Sensing / genetics; Stress, Physiological / genetics; Time Factors
TL;DR: Some of the most interesting genes found to be expressed in milk were correlated with signaling (autoinducer-2) and adherence to mucin and intestinal epithelial cells, in vitro. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2009 article

Toll-like receptor activation profiles of wild-type, recombinant, and mutant Lactobacillus: implications for vaccine design

RETROVIROLOGY, Vol. 6.

By: L. Stoeker n, S. Nordone n, A. LaVoy n, R. Tallon n, T. Klaenhammer n & G. Dean n

TL;DR: This work is investigating the use of Lactobacillus spp. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2008 article

Analysis of the genome sequence of Lactobacillus gasseri ATCC 33323 reveals the molecular basis of an autochthonous intestinal organism

Azcarate-Peril, M. A., Altermann, E., Goh, Y. J., Tallon, R., Sanozky-Dawes, R. B., Pfeiler, E. A., … Klaenhammer, T. R. (2008, August). APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol. 74, pp. 4610–4625.

By: M. Azcarate-Peril n, E. Altermann n, Y. Goh n, R. Tallon n, R. Sanozky-Dawes n, E. Pfeiler n, S. O'Flaherty n, B. Buck n ...

MeSH headings : Adult; Bacterial Adhesion / genetics; Bacterial Adhesion / physiology; Bacterial Proteins / genetics; Child; DNA Primers; Gastrointestinal Tract / microbiology; Genes, Bacterial; Genome, Bacterial; Humans; Infant, Newborn; Intestines / microbiology; Lactobacillus / genetics; Lactobacillus / isolation & purification; Lactobacillus / pathogenicity; Open Reading Frames
TL;DR: The results from this study indicated high intraspecies variability from a genome encoding traits important for survival and retention in the gastrointestinal tract. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2008 article

Functional genomics of probiotic Lactobacilli

Klaenhammer, T. R., Altermann, E., Pfeiler, E., Buck, B. L., Goh, Y.-J., O'Flaherty, S., … Duong, T. (2008, September). JOURNAL OF CLINICAL GASTROENTEROLOGY, Vol. 42, pp. S160–S162.

By: T. Klaenhammer n, E. Altermann n, E. Pfeiler n, B. Buck n, Y. Goh n, S. O'Flaherty n, R. Barrangou n, T. Duong n

author keywords: Lactobacillus acidophilus; Lactobacillus johnsonii; Lactobacillus plantarum; Lactobacillus gasseri; genomics; probiotic cultures; lactic acid bacteria
MeSH headings : Animals; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Evolution, Molecular; Genome, Bacterial; Genomics; Humans; Lactobacillus / classification; Lactobacillus / genetics; Lactobacillus / metabolism; Lactobacillus / physiology; Probiotics / metabolism; Species Specificity
TL;DR: A comparative genomic analysis of 4 completely sequenced Lactobacillus strains versus 25 lactic acid bacterial genomes present in the public database at thetime of analysis is conducted. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2008 article

Genome sequence and characteristics of Lrm1, a prophage from industrial Lactobacillus rhamnosus strain M1

Durmaz, E., Miller, M. J., Azcarate-Peril, M. A., Toon, S. P., & Klaenhammer, T. R. (2008, August). APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol. 74, pp. 4601–4609.

By: E. Durmaz*, M. Miller*, M. Azcarate-Peril*, S. Toon & T. Klaenhammer n

MeSH headings : Aerobiosis; Bacillus / growth & development; Bacillus / virology; DNA, Viral / genetics; DNA, Viral / isolation & purification; DNA, Viral / ultrastructure; Gene Amplification; Genes, Viral / genetics; Genome, Viral; Industry; Kinetics; Microscopy, Electron; Mitomycin / pharmacology; Polymerase Chain Reaction; Prophages / drug effects; Prophages / genetics; Prophages / growth & development; Prophages / ultrastructure
TL;DR: The presence of a defective prophage in an industrial strain could provide superinfection immunity to the host but could also contribute DNA in recombination events to produce new phages potentially infective for the host strain in a large-scale fermentation environment. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2008 patent

Lactobacillus acidophilus nucleic acid sequences encoding carbohydrate utilization-related proteins and uses therefor

By: T. Klaenhammer, E. Altermann, R. Barrangou, W. Russell & T. Duong

Source: NC State University Libraries
Added: August 6, 2018

2008 patent

Lactobacillus acidophilus nucleic acid sequences encoding cell surface protein homologues and uses therefore

Washington, DC: U.S. Patent and Trademark Office.

By: T. Klaenhammer, E. Altermann, B. Buck & W. Russell

Source: NC State University Libraries
Added: August 6, 2018

2008 patent

Lactobacillus acidophilus nucleic acid sequences encoding protease homologues and uses therefore

By: T. Klaenhammer, E. Altermann & W. Russell

Source: NC State University Libraries
Added: August 6, 2018

2008 patent

Lactobacillus acidophilus nucleic acids and uses thereof

By: T. Klaenhammer, A. Azcarate-Peril & E. Altermann

Source: NC State University Libraries
Added: August 6, 2018

2008 journal article

S layer protein A of Lactobacillus acidophilus NCFM regulates immature dendritic cell and T cell functions

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 105(49), 19474–19479.

By: S. Konstantinov*, H. Smidt*, W. Vos*, S. Bruijns*, S. Singh*, F. Valence, D. Molle, S. Lortal ...

author keywords: lactobacilli; DC-SIGN
MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / immunology; Bacterial Proteins / metabolism; Cell Differentiation / immunology; Cell Line; Cytokines / metabolism; Dendritic Cells / immunology; Dendritic Cells / microbiology; Immune Tolerance / immunology; Kidney / cytology; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / immunology; Lactobacillus acidophilus / metabolism; Macrophages / immunology; Macrophages / microbiology; Membrane Glycoproteins / genetics; Membrane Glycoproteins / immunology; Membrane Glycoproteins / metabolism; Mutagenesis; Probiotics; T-Lymphocytes / immunology; T-Lymphocytes / microbiology; Toll-Like Receptor 2 / genetics; Toll-Like Receptor 2 / metabolism; Toll-Like Receptor 4 / genetics; Toll-Like Receptor 4 / metabolism; Transfection
TL;DR: The major S layer protein, SlpA, of L. acidophilus NCFM is the first probiotic bacterial DC-SIGN ligand identified that is functionally involved in the modulation of DCs and T cells functions. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Specific Lactobacillus species differentially activate Toll-like receptors and downstream signals in dendritic cells

EXPERT REVIEW OF VACCINES, 7(8), 1155–1164.

By: M. Mohamadzadeh* & T. Klaenhammer n

author keywords: adjuvant; dendritic cell; Lactobacillus; MAP kinase; Toll-like receptor
MeSH headings : Cells, Cultured; Dendritic Cells / immunology; Gene Expression Profiling; Humans; Interleukin-12 / biosynthesis; Lactobacillus / immunology; Phosphorylation; Toll-Like Receptors / biosynthesis; p38 Mitogen-Activated Protein Kinases / metabolism
TL;DR: It is shown that lactobacilli activate DCs by differentially inducing the expression of Toll-like receptors and bioactive IL-12 in Lactobacillus-treated DCs and may determine the immunological outcome in an environment wherein innate cells reside. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2008 review

Targeting mucosal dendritic cells with microbial antigens from probiotic lactic acid bacteria

[Review of ]. EXPERT REVIEW OF VACCINES, 7(2), 163–174.

By: M. Mohamadzadeh, T. Duong*, T. Hoover & T. Klaenhammer*

MeSH headings : Administration, Oral; Antigens, Bacterial / genetics; Antigens, Bacterial / immunology; Antigens, Bacterial / metabolism; Bacterial Vaccines / administration & dosage; Bacterial Vaccines / genetics; Bacterial Vaccines / metabolism; Dendritic Cells / immunology; Dendritic Cells / microbiology; Genetic Vectors / administration & dosage; Genetic Vectors / genetics; Genetic Vectors / metabolism; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / metabolism; Humans; Immunity, Mucosal; Intestinal Mucosa / immunology; Intestinal Mucosa / microbiology; Lactic Acid / metabolism; Probiotics / administration & dosage; Probiotics / metabolism
TL;DR: This overview of mucosal immunity and how its cellular component(s), particularly dendritic cells, can be specifically targeted to deliver immunogenic subunits, such as the protective antigen from Bacillus anthracis (the causative agent of anthrax) is focused on. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2007 patent

AbiZ phage resistance gene

Washington, DC: U.S. Patent and Trademark Office.

By: E. Durmaz & T. Klaenhammer

Source: NC State University Libraries
Added: August 6, 2018

2007 journal article

Abortive phage resistance mechanism AbiZ speeds the lysis clock to cause premature lysis of phage-infected Lactococcus lactis

JOURNAL OF BACTERIOLOGY, 189(4), 1417–1425.

By: E. Durmaz* & T. Klaenhammer n

MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Bacteriophages; Cloning, Molecular; Lactococcus lactis / metabolism; Lactococcus lactis / virology; Open Reading Frames; Plasmids / genetics; Plasmids / physiology
TL;DR: Results suggest that AbiZ may interact cooperatively with holin to cause premature lysis in phages of the P335 group, through reduction in either plaque size, EOP, or both. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Anti-inflammatory properties of Lactobacillus gasseri expressing manganese superoxide dismutase using the interleukin 10-deficient mouse model of colitis

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 293(4), G729–G738.

By: I. Carroll*, J. Andrus*, J. Bruno-Barcena n, T. Klaenhammer n, H. Hassan n & D. Threadgill

Contributors: I. Carroll*, J. Andrus*, J. Bruno-Bárcena n, T. Klaenhammer n, H. Hassan n & D. Threadgill

author keywords: probiotic; Lactobacilli; oxidative stress; inflammatory bowel disease
MeSH headings : Animals; Anti-Inflammatory Agents / therapeutic use; Colitis / drug therapy; Colitis / pathology; Colon / pathology; Cyclooxygenase 2 / metabolism; Disease Models, Animal; Immunohistochemistry; Interleukin-10 / deficiency; Lactobacillus / enzymology; Mice; Peroxidase / metabolism; Probiotics / therapeutic use; Specific Pathogen-Free Organisms; Superoxide Dismutase / biosynthesis; Superoxide Dismutase / therapeutic use
TL;DR: This study demonstrates that L. gasseri producing MnSOD has significant anti-inflammatory activity that reduces the severity of colitis in the IL-10-deficient mouse. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2007 journal article

Characterization of a novel bile-inducible operon encoding a two-component regulatory system in Lactobacillus acidophilus

JOURNAL OF BACTERIOLOGY, 189(13), 4624–4634.

By: E. Pfeiler n, M. Azcarate-Peril n & T. Klaenhammer n

MeSH headings : Bacterial Proteins / genetics; Base Sequence; Bile Acids and Salts / pharmacology; Gene Expression Regulation, Bacterial / drug effects; Gene Order; Genes, Bacterial / genetics; Lactobacillus acidophilus / drug effects; Lactobacillus acidophilus / genetics; Models, Genetic; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Oligonucleotide Array Sequence Analysis; Reverse Transcriptase Polymerase Chain Reaction; Transcription, Genetic / drug effects
TL;DR: Functional analysis of the effects of bile on the global transcriptional profile of lactobacillus acidophilus NCFM showed that the operon encoded proteins involved in both bile tolerance and bile sensitivity, indicating that the 2CRS plays a role in bileolerance and that theOperon it resides in is negatively controlled by the RR. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Identification of an operon and inducing peptide involved in the production of lactacin B by Lactobacillus acidophilus

JOURNAL OF APPLIED MICROBIOLOGY, 103(5), 1766–1778.

By: A. Dobson n, R. Sanozky-Dawes n & T. Klaenhammer n

author keywords: bacteriocin; class II; inducing peptide; Lactobacillus; two-component regulatory system
MeSH headings : ATP-Binding Cassette Transporters / genetics; Amino Acid Sequence; Bacteriocins / analysis; Bacteriocins / biosynthesis; Bacteriological Techniques; Base Sequence; Cloning, Molecular; Gene Expression Regulation, Bacterial; Intracellular Signaling Peptides and Proteins; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / metabolism; Molecular Sequence Data; Mutagenesis, Insertional; Operon; Peptides / genetics; Sequence Alignment; Sequence Analysis, DNA; Transcription, Genetic
TL;DR: Aim: To determine if a 9·5‐kb region on the Lactobacillus acidophilus NCFM genome, encoded the genetic determinants for regulation and production of lactacin B, a class II bacteriocin. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2007 article

Influence of the dairy environment on gene expression and substrate(1-3)

Klaenhammer, T. R., Azcarate-Peril, M. A., Altermann, E., & Barrangou, R. (2007, March). JOURNAL OF NUTRITION, Vol. 137, pp. 748S–750S.

By: T. Klaenhammer n, M. Azcarate-Peril n, E. Altermann n & R. Barrangou n

MeSH headings : Animals; Cattle; Dairy Products; Dairying; Environment; Gene Expression Regulation, Bacterial; Lactobacillaceae / genetics; Lactobacillaceae / metabolism; Probiotics
TL;DR: Comparative genomics between multiple genomes is providing insights into genes that are important in metabolic, physiological, and functional roles for different LAB in the environments they inhabit, ranging from the gastrointestinal tract to milk and acidified dairy products. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2007 journal article

Inhibition of bacteriophage replication in Streptococcus thermophilus by subunit poisoning of primase

MICROBIOLOGY-SGM, 153, 3295–3302.

By: J. Sturino n & T. Klaenhammer n

MeSH headings : Amino Acid Sequence; Amino Acid Substitution / genetics; Codon, Nonsense; DNA Primase / antagonists & inhibitors; DNA Primase / genetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Structure, Tertiary; Protein Subunits / genetics; Streptococcus Phages / growth & development; Streptococcus thermophilus / virology; Viral Plaque Assay; Virus Replication / physiology
TL;DR: This study appears to represent the first use of transdominant proteins to inhibit phages that are disruptive to cultures used in industrial fermentations. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2007 journal article

Modification of Lactobacillus beta-glucuronidase activity by random mutagenesis

GENE, 389(2), 122–127.

By: M. Callanan n, W. Russell n & T. Klaenhammer n

author keywords: Lactobacillus gasseri gusA; gene; reporter enzyme; pH range; Epicurian coli
MeSH headings : Amino Acid Sequence; Amino Acid Substitution; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Cloning, Molecular; DNA, Bacterial; Escherichia coli / enzymology; Escherichia coli / genetics; Genes, Bacterial; Glucuronidase / chemistry; Glucuronidase / genetics; Glucuronidase / metabolism; Hydrogen-Ion Concentration; Lactobacillus / enzymology; Lactobacillus / genetics; Molecular Sequence Data; Mutagenesis; Sequence Alignment; Sequence Analysis, DNA; Transformation, Bacterial
TL;DR: The modified GusA3 enzyme has expanded potential for use as a reporter enzyme in expression hosts that are not acidophilic, as well as lactic acid bacteria and other microorganisms that grow in acidifying environments. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2007 review

The genomics of lactic acid bacteria

[Review of ]. TRENDS IN MICROBIOLOGY, 15(12), 546–553.

By: E. Pfeiler* & T. Klaenhammer*

MeSH headings : Dairy Products / microbiology; Genome, Bacterial; Gram-Positive Bacteria / classification; Gram-Positive Bacteria / genetics; Gram-Positive Bacteria / metabolism; Humans; Lactic Acid / biosynthesis; Lactic Acid / metabolism; Probiotics
TL;DR: A review of the genome sequencing of 20 lactic acid bacteria provides an expanded view of their genetic and metabolic capacities and enables researchers to perform functional and comparative genomic studies. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Analysis of treatment effects on the microbial ecology of the human intestine

FEMS MICROBIOLOGY ECOLOGY, 57(2), 239–250.

By: A. Engelbrektson*, J. Korzenik*, M. Sanders, B. Clement*, G. Leyer, T. Klaenhammer n, C. Kitts*

author keywords: terminal restriction fragment length polymorphism; probiotics; antibiotics; prebiotics; gastrointestinal microbiota
MeSH headings : Adult; Anti-Bacterial Agents / administration & dosage; Bacteria / classification; Bacteria / drug effects; Bacteria / genetics; Cluster Analysis; DNA Fingerprinting; DNA, Bacterial / genetics; Double-Blind Method; Feces / microbiology; Humans; Intestines / microbiology; Placebos; Polymorphism, Restriction Fragment Length; Principal Component Analysis; Probiotics / administration & dosage
TL;DR: Two different human trials where the fecal terminal restriction fragment length polymorphism data sets were analyzed to search for treatment effects show a clearer indication of treatment effects than did an evaluation of data grouped before analysis. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Characterization of the tre locus and analysis of trehalose cryoprotection in Lactobacillus acidophilus NCFM

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72(2), 1218–1225.

By: T. Duong n, R. Barrangou n, W. Russell & T. Klaenhammer n

MeSH headings : Amino Acid Sequence; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Base Sequence; Cryoprotective Agents / metabolism; Cryoprotective Agents / pharmacology; DNA, Bacterial / genetics; Disaccharidases / genetics; Disaccharidases / metabolism; Fermentation; Genes, Bacterial; Lactobacillus acidophilus / drug effects; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / metabolism; Molecular Sequence Data; Mutagenesis, Insertional; Mutation; Phosphoenolpyruvate Sugar Phosphotransferase System / genetics; Phosphoenolpyruvate Sugar Phosphotransferase System / metabolism; Repressor Proteins / genetics; Repressor Proteins / metabolism; Trehalose / metabolism; Trehalose / pharmacology
TL;DR: Analysis of the Lactobacillus acidophilus NCFM genome revealed a putative trehalose utilization locus, which was found to be significantly reduced in the transporter mutant but unaffected in the hydrolase mutant, suggesting that intracellular transport and hydrolysis contribute significantly to cryoprotection. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2006 journal article

Comparative genomics and transcriptional analysis of prophages identified in the Genomes of Lactobacillus gasseri, Lactobacillus salivarius, and Lactobacillus casei

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72(5), 3130–3146.

By: M. Ventura n, C. Canchaya*, V. Bernini n, E. Altermann n, R. Barrangou n, S. McGrath*, M. Claesson*, Y. Li* ...

MeSH headings : Animals; Base Sequence; Genome, Bacterial; Genome, Viral; Genomics; Humans; Lactobacillus / genetics; Lactobacillus / virology; Lacticaseibacillus casei / virology; Molecular Sequence Data; Phylogeny; Prophages / classification; Prophages / genetics; Prophages / isolation & purification; Prophages / metabolism; Sequence Analysis, DNA; Siphoviridae / classification; Siphoviridae / genetics; Siphoviridae / isolation & purification; Siphoviridae / metabolism; Transcription, Genetic; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR: Phylogenetic investigation of these newly described prophage sequences revealed that they have not followed an evolutionary development similar to that of their bacterial hosts and that they show a high degree of diversity, even within a species. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2006 journal article

Comparative genomics of the lactic acid bacteria

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 103(42), 15611–15616.

By: K. Makarova*, A. Slesarev*, Y. Wolf*, A. Sorokin*, B. Mirkin*, E. Koonin*, A. Pavlov*, N. Pavlova ...

author keywords: evolutionary genomics; fermentation
MeSH headings : Animals; Bacterial Proteins / classification; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Biological Evolution; Food Microbiology; Gene Transfer, Horizontal; Genome, Bacterial; Genomics; Lactic Acid / metabolism; Lactobacillus / classification; Lactobacillus / genetics; Phylogeny; Streptococcaceae / classification; Streptococcaceae / genetics
TL;DR: Phylogenetic analyses, comparison of gene content across the group, and reconstruction of ancestral gene sets indicate a combination of extensive gene loss and key gene acquisitions via horizontal gene transfer during the coevolution of lactic acid bacteria with their habitats. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2006 review

Engineered bacteriophage-defence systems in bioprocessing

[Review of ]. NATURE REVIEWS MICROBIOLOGY, 4(5), 395–404.

By: J. Sturino n & T. Klaenhammer n

MeSH headings : Bacteria / genetics; Bacteria / virology; Bacteriophages / physiology; Biotechnology; Dairy Products; Fermentation; Food Microbiology; Genetic Engineering; Genomics; RNA, Antisense; Streptococcus / genetics; Streptococcus / physiology; Streptococcus / virology; Streptococcus Phages / genetics; Streptococcus Phages / physiology; Virus Replication
TL;DR: A set of genetic tools that are useful in the engineering of customized phage-defence systems are described, with special focus on the power of comparative genomics as a means of streamlining target selection, providing more widespread phage protection, and increasing the longevity of these industrially important bacteria in the bioprocessing environment. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Global analysis of carbohydrate utilization by Lactobacillus acidophilus using cDNA microarrays

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 103(10), 3816–3821.

By: R. Barrangou*, M. Azcarate-Peril*, T. Duong*, S. Conners n, R. Kelly n & T. Klaenhammer*

author keywords: ATP-binding cassette; carbon catabolite repression; fructooligosaccharide; galactoside-pentose hexuronide
MeSH headings : ATP-Binding Cassette Transporters / genetics; ATP-Binding Cassette Transporters / metabolism; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Carbohydrate Metabolism / genetics; Gastrointestinal Tract / microbiology; Gene Expression Profiling; Genes, Bacterial; Humans; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / metabolism; Models, Biological; Oligonucleotide Array Sequence Analysis; Phosphoenolpyruvate Sugar Phosphotransferase System / genetics; Reverse Transcriptase Polymerase Chain Reaction
TL;DR: The transport and catabolic machinery involved in carbohydrate utilization by Lactobacillus acidophilus was characterized genetically by using whole-genome cDNA microarrays, and genes central to glycolysis were among the most highly expressed in the genome. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2006 journal article

Transcriptional and functional analysis of oxalyl-coenzyme A (CoA) decarboxylase and formyl-CoA transferase genes from Lactobacillus acidophilus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72(3), 1891–1899.

By: M. Azcarate-Peril n, J. Bruno-Barcena*, H. Hassan* & T. Klaenhammer n

Contributors: M. Azcarate-Peril n, J. Bruno-Bárcena*, H. Hassan* & T. Klaenhammer n

MeSH headings : Carboxy-Lyases / genetics; Carboxy-Lyases / metabolism; Coenzyme A-Transferases / genetics; Coenzyme A-Transferases / metabolism; Gene Expression Regulation, Bacterial; Lactobacillus acidophilus / enzymology; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / growth & development; Oligonucleotide Array Sequence Analysis; Operon; Oxalates / metabolism; Reverse Transcriptase Polymerase Chain Reaction; Transcription, Genetic
TL;DR: Transcriptional analysis using cDNA microarrays and reverse transcription-quantitative PCR revealed that mildly acidic conditions were a prerequisite for frc and oxc transcription, andOxalate-dependent induction of these genes occurred only in cells first adapted to subinhibitory concentrations of oxalate and then exposed to pH 5.5. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2005 journal article

Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 102(11), 3906–3912.

By: E. Altermann n, W. Russell n, M. Azcarate-Peril n, R. Barrangou n, B. Buck n, O. McAuliffe n, N. Souther n, A. Dobson n ...

author keywords: adhesion; stress response; proteolytic system; sugar metabolism; in silico analysis
MeSH headings : Bacterial Adhesion / genetics; Bacteriocins / genetics; Bacteriophages / genetics; Bacteriophages / pathogenicity; Carbohydrate Metabolism; Computational Biology; Genome, Bacterial; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / metabolism; Lactobacillus acidophilus / virology; Molecular Sequence Data; Peptide Hydrolases / genetics; Prophages / genetics; Sequence Analysis, DNA; Transposases / genetics
TL;DR: These features within the genome sequence of L. acidophilus are likely to contribute to the organisms' gastric survival and promote interactions with the intestinal mucosa and microbiota. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2005 journal article

Functional analysis of putative adhesion factors in Lactobacillus acidophilus NCFM

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(12), 8344–8351.

By: B. Buck n, E. Altermann n, T. Svingerud n & T. Klaenhammer n

MeSH headings : Adhesins, Bacterial / genetics; Adhesins, Bacterial / physiology; Base Sequence; DNA Primers; DNA, Bacterial / genetics; Escherichia coli / genetics; Genome, Bacterial; Lactobacillus acidophilus / classification; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / physiology; Mucins / metabolism; Phylogeny; Plasmids; Restriction Mapping
TL;DR: It is demonstrated that multiple cell surface proteins in L. acidophilus NCFM can individually contribute to the organism's ability to attach to intestinal cells in vitro. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Genetic analysis of two bile salt hydrolase activities in Lactobacillus acidophilus NCFM

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(8), 4925–4929.

By: O. McAuliffe n, R. Cano n & T. Klaenhammer n

MeSH headings : Amidohydrolases / genetics; Amidohydrolases / metabolism; Bacterial Proteins / genetics; Bile Acids and Salts / metabolism; Bile Acids and Salts / pharmacology; Chromosomes, Bacterial; Culture Media; DNA, Bacterial / genetics; Gene Deletion; Genetic Vectors; Humans; Lactobacillus acidophilus / drug effects; Lactobacillus acidophilus / enzymology; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / growth & development; Substrate Specificity
TL;DR: Targeted inactivation of these genes via chromosomal insertion of an integration vector demonstrated different substrate specificities for these two enzymes. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Genomic Perspectives on Probiotic Lactic Acid Bacteria

Bioscience and Microflora, 24(2), 31–33.

By: T. Klaenhammer n, A. Peril n, R. Barrangou n, T. Duong n & E. Altermann n

TL;DR: The genomic content of probiotic Lactobacillus cultures is examined, compared to those lactic acid bacterial genomes that have appeared recently, to illustrate how environmental conditions encountered during biomanufacturing, fermentation, and the gastrointestinal tract can impact gene expression and culture functionality. (via Semantic Scholar)
Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2005 review

Genomic features of lactic acid bacteria effecting bioprocessing and health

[Review of ]. FEMS MICROBIOLOGY REVIEWS, 29(3), 393–409.

By: T. Klaenhammer n, R. Barrangou n, B. Buck n, M. Azcarate-Peril n & E. Altermann n

author keywords: lactic acid bacteria; genomics; bioprocessing; probiotics; comparative genomics; functional genomics
MeSH headings : Food Microbiology; Genome, Bacterial; Genomics; Health; Humans; Lactobacillus / genetics; Lactobacillus / growth & development; Lactobacillus / physiology; Lactococcus / genetics; Lactococcus / growth & development; Lactococcus / physiology; Probiotics
TL;DR: Genomic analyses of multiple members of the lactic acid bacteria have now elucidated many genetic features that direct their fermentative and probiotic roles, providing an important platform for understanding core mechanisms that control and regulate bacterial growth, survival, signaling, and fermentative processes. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2005 journal article

Lactobacilli activate human dendritic cells that skew T cells toward T helper 1 polarization

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 102(8), 2880–2885.

By: M. Mohamadzadeh n, S. Olson n, W. Kalina n, G. Ruthel n, G. Demmin n, K. Warfield n, S. Bavari n, T. Klaenhammer n

MeSH headings : Cell Polarity; Dendritic Cells / physiology; Humans; Interleukin-12 / biosynthesis; Interleukin-18 / biosynthesis; Lactobacillus / physiology; Lymphocyte Activation; Membrane Glycoproteins / genetics; Membrane Glycoproteins / physiology; Receptors, Cell Surface / genetics; Receptors, Cell Surface / physiology; T-Lymphocytes / immunology; Th1 Cells / physiology; Toll-Like Receptors; Tumor Necrosis Factor-alpha / biosynthesis
TL;DR: The results emphasize a potentially important role for lactobacilli in modulating immunological functions of DCs and suggest that certain strains could be particularly advantageous as vaccine adjuvants, by promoting DCs to regulate T cell responses toward T helper 1 and Tc1 pathways. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Marker-free chromosomal integration of the manganese superoxide dismutase gene (sodA) from Streptococcus thermophilus into Lactobacillus gasseri

FEMS MICROBIOLOGY LETTERS, 246(1), 91–101.

By: J. Bruno-Barcena n, M. Azcarate-Peril n, T. Klaenhammer n & H. Hassan n

Contributors: J. Bruno-Bárcena n, M. Azcárate-Peril n, T. Klaenhammer n & H. Hassan n

author keywords: functional gene replacement; manganese superoxide dismutase; oxidative stress; Lactobacillus gasseri; lactic acid bacteria; probiotics
MeSH headings : Anti-Bacterial Agents / pharmacology; Bacterial Proteins / analysis; Chromosomes, Bacterial / genetics; Gene Expression; Hydrogen Peroxide / pharmacology; Lactobacillus / enzymology; Lactobacillus / genetics; Lactobacillus / growth & development; Plasmids / genetics; RNA, Bacterial / analysis; RNA, Messenger / analysis; Streptococcus thermophilus / enzymology; Streptococcus thermophilus / genetics; Superoxide Dismutase / genetics; Transformation, Bacterial
TL;DR: This is the first report showing the successful utilization of the pORI plasmids system to generate marker-free gene integration in L. gasseri strains. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2005 journal article

Microarray analysis of a two-component regulatory system involved in acid resistance and proteolytic activity in Lactobacillus acidophilus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(10), 5794–5804.

By: M. Azcarate-Peril n, O. McAuliffe n, E. Altermann n, S. Lick n, W. Russell* & T. Klaenhammer n

MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Base Sequence; Culture Media; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Histidine Kinase; Hydrogen-Ion Concentration; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / growth & development; Lactobacillus acidophilus / metabolism; Lactobacillus acidophilus / physiology; Mutation; Oligonucleotide Array Sequence Analysis / methods; Peptide Hydrolases / genetics; Peptide Hydrolases / metabolism; Protein Kinases / genetics; Protein Kinases / metabolism; Signal Transduction
Source: Web Of Science
Added: August 6, 2018

2005 review

Modulation of the microbial ecology of the human colon by probiotics, prebiotics and synbiotics to enhance human health: An overview of enabling science and potential applications

[Review of ]. FEMS MICROBIOLOGY ECOLOGY, 52(2), 145–152.

By: R. Rastall*, G. Gibson*, H. Gill, F. Guarner, T. Klaenhammer n, B. Pot*, G. Reid*, I. Rowland*, M. Sanders

author keywords: probiotics; prebiotics; synbiotics; colonic microbiota
MeSH headings : Colon / drug effects; Colon / immunology; Colon / microbiology; Developing Countries; Drug and Narcotic Control; Humans; Probiotics / chemistry; Probiotics / economics; Probiotics / pharmacology; Probiotics / therapeutic use
TL;DR: Increasing knowledge of the metabolism of prebiotics by probiotics is allowing us to consider specifically targeting such dietary intervention tools at specific population groups and specific disease states. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

PathwayVoyager: pathway mapping using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database

BMC Genomics, 6.

By: E. Altermann & T. Klaenhammer

Source: NC State University Libraries
Added: August 6, 2018

2005 journal article

Sequence analysis of the Lactobacillus plantarum bacteriophage Phi JL-1

GENE, 348, 45–54.

By: Z. Lu*, E. Altermann n, F. Breidt n, P. Predki, H. Fleming* & T. Klaenhammer n

author keywords: bacteriophage; Phi JL-1; sequence analysis; structural proteins; Lactobacillus plantarum
MeSH headings : Amino Acid Sequence; Bacteriolysis / genetics; Bacteriophages / genetics; Bacteriophages / ultrastructure; Chromosome Mapping; DNA Replication / genetics; DNA, Viral / chemistry; DNA, Viral / genetics; Genes / genetics; Genome, Viral; Lactobacillus plantarum / virology; Microscopy, Electron; Molecular Sequence Data; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Viral Structural Proteins / genetics; Virus Assembly / genetics
TL;DR: The structural gene maps revealed that the order of the head and tail genes is highly conserved among the genomes of several Siphoviridae phages, allowing the assignment of probable functions to certain uncharacterized ORFs from phage PhiJL-1 and other Siphaviridaephages. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2004 patent

Antisense RNA expression strategies effective against Streptococcus thermophilus bacteriophages

Washington, DC: U.S. Patent and Trademark Office.

By: J. Sturino & T. Klaenhammer

Source: NC State University Libraries
Added: August 6, 2018

2004 journal article

Antisense RNA targeting of primase interferes with bacteriophage replication in Streptococcus thermophilus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(3), 1735–1743.

By: J. Sturino n & T. Klaenhammer n

MeSH headings : Base Sequence; DNA Primase / genetics; DNA Replication; DNA, Viral / genetics; Genes, Viral; Genetic Vectors; Molecular Sequence Data; Mutation; RNA, Antisense / genetics; RNA, Viral / genetics; Streptococcus / virology; Streptococcus Phages / enzymology; Streptococcus Phages / genetics; Streptococcus Phages / physiology; Virus Replication
TL;DR: Targeting a key genome replication function with antisense RNA provided effective phage protection in S. thermophilus. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2004 review

Bacteriophage defense systems and strategies for lactic acid bacteria

[Review of ]. ADVANCES IN APPLIED MICROBIOLOGY, VOL 56, 56, 331-+.

By: J. Sturino* & T. Klaenhammer n

MeSH headings : Animals; Bacteriophages / genetics; Bacteriophages / physiology; Cattle; Dairying; Humans; Lactococcus lactis / genetics; Lactococcus lactis / virology; Streptococcus Phages / genetics; Streptococcus Phages / physiology; Streptococcus thermophilus / genetics; Streptococcus thermophilus / virology
TL;DR: This chapter highlights the various defense strategies and systems that have been developed to curb the propagation and evolution of lytic phages in S. thermophilus and their bacteriophages. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2004 journal article

DNA analysis of the genes encoding acidocin LF221 A and acidocin LF221 B, two bacteriocins produced by Lactobacillus gasseri LF221

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 63(6), 705–714.

By: A. Majhenic*, K. Venema*, G. Allison*, B. Matijasic*, I. Rogelj* & T. Klaenhammer n

MeSH headings : Amino Acid Sequence; Bacteriocins / chemistry; Bacteriocins / genetics; Base Sequence; Cloning, Molecular; Codon, Initiator; Codon, Terminator; DNA, Bacterial / chemistry; DNA, Bacterial / isolation & purification; Genes, Bacterial; Lactobacillus / genetics; Molecular Sequence Data; Open Reading Frames; Plasmids; Probiotics; Promoter Regions, Genetic; Protein Sorting Signals / genetics; Protein Sorting Signals / physiology; Sequence Analysis, DNA; Sequence Homology
TL;DR: Assessment of the genetic information on both acidocins of L. gasseri LF221 shows them to be members of the two-component class II bacteriocins, where acidocin LF221 A appears to be a novel bacteriOCin. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Epidemic clone I-specific genetic markers in strains of Listeria monocytogenes serotype 4b from foods

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(7), 4158–4164.

By: S. Yildirim n, W. Lin*, A. Hitchins*, L. Jaykus n, E. Altermann n, T. Klaenhammer n, S. Kathariou n

MeSH headings : Base Composition; DNA, Bacterial / analysis; Food Microbiology; Genetic Markers; Listeria monocytogenes / classification; Listeria monocytogenes / genetics; Serotyping
TL;DR: Examination of 34 serogroup 4 (putative or confirmed serotype 4b) isolates of L. monocytogenes obtained from various foods and food-processing environments, without known implication in illness, revealed that many of these strains had methylation of cytosines at GATC sites in the genome, rendering their DNA resistant to digestion by the restriction endonuclease Sau3AI. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2004 article

Epidemic clone I-specific genetic markers in strains of Listeria monocytogenes serotype 4b from foods (vol 70, pg 4158, 2004)

Yildirim, S., Lin, W., Hitchins, A. D., Jaykus, L. A., Altermann, E., Klaenhammer, T. R., & Kathariou, S. (2004, December). APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Vol. 70, pp. 7581–7581.

By: S. Yildirim n, W. Lin n, A. Hitchins n, L. Jaykus n, E. Altermann n, T. Klaenhammer n, S. Kathariou n

UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Expression of a heterologous manganese superoxide dismutase gene in intestinal lactobacilli provides protection against hydrogen peroxide toxicity

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(8), 4702–4710.

By: J. Bruno-Barcena*, J. Andrus*, S. Libby*, T. Klaenhammer n & H. Hassan n

Contributors: J. Bruno-Bárcena*, J. Andrus*, S. Libby*, T. Klaenhammer n & H. Hassan n

MeSH headings : Cloning, Molecular; Humans; Hydrogen Peroxide / pharmacology; Intestines / microbiology; Lactobacillus / classification; Lactobacillus / drug effects; Lactobacillus / enzymology; Lactobacillus / genetics; Oxidative Stress / drug effects; Protein Biosynthesis; Streptococcus / enzymology; Streptococcus / genetics; Superoxide Dismutase / genetics; Superoxide Dismutase / metabolism; Transcription, Genetic
TL;DR: The data show that MnSOD protects cells against hydrogen peroxide by removing O2·− and preventing the redox cycling of iron and is the first report of a sodA from S. thermophilus being expressed in other lactic acid bacteria. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2004 journal article

Genetic markers unique to Listeria monocytogenes serotype 4b differentiate epidemic clone II (hot dog outbreak strains) from other lineages

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(4), 2383–2390.

By: M. Evans n, B. Swaminathan*, L. Graves*, E. Altermann n, T. Klaenhammer n, R. Fink n, S. Kernodle n, S. Kathariou n

MeSH headings : Animals; Base Sequence; DNA Primers / genetics; DNA, Bacterial / genetics; Disease Outbreaks; Europe / epidemiology; Food Microbiology; Foodborne Diseases / epidemiology; Foodborne Diseases / microbiology; Genes, Bacterial; Genetic Markers; Humans; Listeria monocytogenes / classification; Listeria monocytogenes / genetics; Listeria monocytogenes / isolation & purification; Listeria monocytogenes / pathogenicity; Meat Products / microbiology; North America / epidemiology; Polymerase Chain Reaction; Serotyping
TL;DR: The findings suggest that ECII strains have undergone divergence in portions of a serotype-specific region that is conserved in other serotype 4b strains, although the mechanisms that drive this divergence remain to be identified. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Identification and inactivation of genetic loci involved with Lactobacillus acidophilus acid tolerance

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(9), 5315–5322.

MeSH headings : Base Sequence; Calorimetry; DNA Primers; Genome, Bacterial; Hydrogen-Ion Concentration; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / growth & development; Molecular Sequence Data; Polymerase Chain Reaction; Restriction Mapping
TL;DR: Interestingly, each integrant also exhibited an adaptive acid response during logarithmic growth, indicating that multiple mechanisms are present and orchestrated in L. acidophilus in response to acid challenge. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2004 journal article

Identification and phenotypic characterization of the cell-division protein CdpA

GENE, 342(1), 189–197.

By: E. Altermann n, B. Buck n, R. Cano* & T. Klaenhammer n

author keywords: lactic acid bacteria; lactobacillus; cell adhesion; stress tolerance; functional inactivation; cell wall; cell separation
MeSH headings : Bacterial Adhesion / genetics; Bacterial Adhesion / physiology; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Caco-2 Cells; Cell Cycle Proteins / genetics; Cell Division / drug effects; Cell Division / genetics; Cell Division / physiology; Cell Wall / metabolism; Culture Media / pharmacology; DNA, Bacterial / chemistry; DNA, Bacterial / genetics; DNA, Bacterial / isolation & purification; Ethanol / pharmacology; Freezing; Gene Silencing; Humans; Lactobacillus acidophilus / drug effects; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / growth & development; Membrane Glycoproteins / genetics; Molecular Sequence Data; Mutation; Phylogeny; Sequence Analysis, DNA; Sodium Chloride / pharmacology; Temperature
TL;DR: It is ascertained that this protein plays a role in cell-wall processing during the growth and cell-cell separation and is designated as cell-division protein, cdpA, based on the in silico and functional analyses. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2004 patent

Lactobacillus acidophilus nucleic acids encoding fructo-oligosaccharide utilization compounds and uses thereof

Washington, DC: U.S. Patent and Trademark Office.

By: R. Barrangou, T. Klaenhammer & E. Altermann

Source: NC State University Libraries
Added: August 6, 2018

2004 journal article

The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 101(8), 2512–2517.

By: R. Pridmore n, B. Berger n, F. Desiere n, D. Vilanova n, C. Barretto n, A. Pittet n, M. Zwahlen n, M. Rouvet n ...

MeSH headings : Biological Transport; Cell Adhesion; Energy Metabolism; Fimbriae, Bacterial / genetics; Genes, Bacterial / genetics; Genome, Bacterial; Humans; Intestinal Mucosa / microbiology; Lactobacillus / genetics; Lactobacillus / metabolism; Lactobacillus / pathogenicity; Molecular Sequence Data; Operon / genetics
TL;DR: Genome analysis predicted an abundance of large and unusual cell-surface proteins, including fimbrial subunits, which may be involved in adhesion to glycoproteins or other components of mucin, a characteristic expected to affect persistence in the gastrointestinal tract (GIT). (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2003 journal article

A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes

NUCLEIC ACIDS RESEARCH, 31(7), 1805–1812.

By: R. Roberts, M. Belfort*, T. Bestor*, A. Bhagwat*, T. Bickle*, J. Bitinaite, R. Blumenthal*, S. Degtyarev ...

MeSH headings : Base Sequence; Binding Sites; DNA / genetics; DNA / metabolism; DNA Restriction Enzymes / classification; DNA Restriction Enzymes / genetics; DNA Restriction Enzymes / metabolism; Methyltransferases / classification; Methyltransferases / genetics; Methyltransferases / metabolism; Terminology as Topic
TL;DR: A nomenclature is described for restriction endon nucleases, DNA methyltransferases, homing endonucleases and related genes and gene products that provides explicit categories for the many different Type II enzymes now identified and provides a system for naming the putative genes found by sequence analysis of microbial genomes. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Analysis, characterization, and loci of the tuf genes in Lactobacillus and Bifidobacterium species and their direct application for species identification

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 69(11), 6908–6922.

By: M. Ventura*, C. Canchaya*, V. Meylan*, T. Klaenhammer n & R. Zink*

MeSH headings : Adult; Animals; Bacterial Typing Techniques; Bifidobacterium / classification; Bifidobacterium / genetics; Cattle; Child; DNA, Bacterial / analysis; DNA, Ribosomal / analysis; Humans; Lactobacillus / classification; Lactobacillus / genetics; Molecular Sequence Data; Peptide Elongation Factor Tu / genetics; Phylogeny; RNA, Ribosomal, 16S / genetics; Sequence Analysis, DNA; Species Specificity; Transcription, Genetic
TL;DR: It is demonstrated that the synonymous substitution affecting this gene renders elongation factor Tu a reliable molecular clock for investigating evolutionary distances of lactobacilli and bifidobacteria. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Functional and comparative genomic analyses of an operon involved in fructooligosaccharide utilization by Lactobacillus acidophilus

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 100(15), 8957–8962.

By: R. Barrangou n, E. Altermann n, R. Hutkins n, R. Cano n & T. Klaenhammer n

MeSH headings : ATP-Binding Cassette Transporters / genetics; ATP-Binding Cassette Transporters / metabolism; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Base Sequence; Biological Transport, Active; Chromosome Mapping; DNA, Bacterial / genetics; Escherichia coli Proteins / genetics; Escherichia coli Proteins / metabolism; Genome, Bacterial; Lac Repressors; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / metabolism; Molecular Sequence Data; Multigene Family; Mutation; Oligosaccharides / metabolism; Operon; Phenotype; Phylogeny; Repressor Proteins / genetics; Repressor Proteins / metabolism; Sequence Homology, Nucleic Acid; Species Specificity
TL;DR: This is a description of a previously undescribed gene locus involved in transport and catabolism of FOS compounds, which can promote competition of beneficial microorganisms in the human gastrointestinal tract. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2003 journal article

Isolation and characterization of a Lactobacillus plantarum bacteriophage, Phi JL-1, from a cucumber fermentation

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 84(2), 225–235.

By: Z. Lu n, F. Breidt n, H. Fleming n, E. Altermann n & T. Klaenhammer n

author keywords: bacteriophage; Lactobacillus plantarum; lactic acid bacteria; vegetable fermentation; cucumber fermentation
MeSH headings : Adsorption; Bacteriophages / genetics; Bacteriophages / isolation & purification; Bacteriophages / physiology; Cucumis sativus / microbiology; Culture Media / chemistry; DNA, Viral / analysis; Electrophoresis, Polyacrylamide Gel; Fermentation; Food Microbiology; Genome, Viral; Hydrogen-Ion Concentration; Kinetics; Lactobacillus / virology
TL;DR: A virulent Lactobacillus plantarum bacteriophage, PhiJL-1, was isolated from a commercial cucumber fermentation and sequenced to reveal a linear, double-stranded DNA that may be important for the development of controlled vegetable fermentations. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Molecular characterization and functional analysis of the manganese-containing superoxide dismutase gene (sodA) from Streptococcus thermophilus AO54

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 420(1), 103–113.

By: J. Andrus n, S. Bowen n, T. Klaenhammer n & H. Hassan n

author keywords: Streptococcus thermophilus; superoxide dismutase; MnSOD; sodA sequence; sodA mutant; SOD-null mutant; oxygen sensitivity; manganese ions; DNA polymerase III
MeSH headings : Amino Acid Sequence; Gene Expression Regulation, Enzymologic / drug effects; Magnesium Chloride / pharmacology; Molecular Sequence Data; Mutagenesis, Site-Directed; Sequence Alignment; Sequence Analysis, Protein; Species Specificity; Streptococcus / chemistry; Streptococcus / drug effects; Streptococcus / enzymology; Streptococcus / genetics; Structure-Activity Relationship; Superoxide Dismutase / chemistry; Superoxide Dismutase / genetics; Superoxide Dismutase / metabolism
TL;DR: The results suggest that sodA is essential for the aerobic growth of S. thermophilus and in the absence of functional SodA, manganese ions may provide partial protection against oxygen toxicity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID
Added: August 6, 2018

2003 patent

Polynucleotide encoding a Lactobacillus gasseri beta-glucuronidase polypeptide

Washington, DC: U.S. Patent and Trademark Office.

By: W. Russell & T. Klaenhammer

Source: NC State University Libraries
Added: August 6, 2018

2002 journal article

Characterization of six Leuconostoc fallax bacteriophages isolated from an industrial sauerkraut fermentation

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(11), 5452–5458.

By: R. Barrangou n, S. Yoon n, F. Breidt n, H. Fleming n & T. Klaenhammer n

MeSH headings : Bacteriophages / genetics; Bacteriophages / growth & development; Bacteriophages / isolation & purification; Bacteriophages / metabolism; Bacteriophages / ultrastructure; DNA Fingerprinting; DNA, Viral / analysis; Fermentation; Genome, Viral; Leuconostoc / virology; Polymorphism, Restriction Fragment Length; Random Amplified Polymorphic DNA Technique; Viral Proteins / analysis
TL;DR: Bacteriophages active against Leuconostoc fallax strains are likely to contribute to the microbial ecology of sauerkraut fermentation and could be responsible for some of the variability observed in this type of fermentation. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2002 journal article

Discovering lactic acid bacteria by genomics

Antonie Van Leeuwenhoek, 82(1-4), 29–58.

By: T. Klaenhammer n, E. Altermann n, F. Arigoni*, A. Bolotin*, F. Breidt n, J. Broadbent*, R. Cano*, S. Chaillou ...

MeSH headings : Bifidobacterium / genetics; Fermentation; Genomics; Gram-Positive Bacteria / genetics; Lactobacillus / classification; Lactobacillus / genetics; Lactococcus lactis / classification; Lactococcus lactis / genetics
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018

2002 journal article

Expression of antisense RNA targeted against Streptococcus thermophilus bacteriophages

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(2), 588–596.

By: J. Sturino n & T. Klaenhammer n

MeSH headings : Amino Acid Sequence; Blotting, Southern; DNA Helicases / genetics; DNA Helicases / metabolism; DNA Replication; Gene Silencing; Genetic Vectors; Molecular Sequence Data; RNA, Antisense / genetics; RNA, Antisense / metabolism; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA; Streptococcus / virology; Streptococcus Phages / genetics; Streptococcus Phages / physiology; Viral Plaque Assay; Virus Replication
TL;DR: These results illustrate the first successful applications of antisense RNA and explosive delivery of antisensing RNA to inhibit the proliferation of S. thermophilus bacteriophages. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Food research trends - 2003 and beyond

Food Technology, 56(12), 30-.

By: N. Mermelstein, O. Fennema, C. Batt, H. Goff, M. Griffiths, D. Hoover, F. Hsieh, V. Juneja ...

Source: NC State University Libraries
Added: August 6, 2018

2002 journal article

Identification and characterization of Leuconostoc fallax strains isolated from an industrial sauerkraut fermentation

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(6), 2877–2884.

By: R. Barrangou*, S. Yoon*, F. Breidt n, H. Fleming n & T. Klaenhammer*

MeSH headings : Brassica / microbiology; Cell Culture Techniques; DNA, Intergenic / analysis; Fermentation; Industry; Leuconostoc / genetics; Leuconostoc / isolation & purification; Plant Extracts; Polymerase Chain Reaction; RNA, Ribosomal, 16S / analysis; RNA, Ribosomal, 16S / genetics; Random Amplified Polymorphic DNA Technique
Sources: Web Of Science, ORCID
Added: August 6, 2018

2002 journal article

Isolation and characterization of bacteriophages from fermenting sauerkraut

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(2), 973–976.

By: S. Yoon*, R. Barrangou-Poueys, F. Breidt n, T. Klaenhammer n & H. Fleming n

MeSH headings : Bacteriophages / classification; Bacteriophages / isolation & purification; Brassica; Fermentation; Food-Processing Industry / instrumentation; Food-Processing Industry / methods; Lactobacillus / virology; Leuconostoc / virology; Myoviridae / classification; Myoviridae / isolation & purification; Siphoviridae / classification; Siphoviridae / isolation & purification
TL;DR: The first report of bacteriophage isolated from commercial vegetable fermentations is presented, and these phages were active against fermentation isolates and selected Leuconostoc mesenteroides and Lactobacillus plantarum strains, including a starter culture. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2002 journal article

Lactococcus lactis lytic bacteriophages of the p335 group are inhibited by overexpression of a truncated CI repressor

JOURNAL OF BACTERIOLOGY, 184(23), 6532–6543.

By: E. Durmaz n, S. Madsen n, H. Israelsen n & T. Klaenhammer n

MeSH headings : Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA-Binding Proteins; Gene Expression Regulation, Viral; Lactococcus lactis / virology; Lysogeny; Molecular Sequence Data; Mutation; Operator Regions, Genetic; Repressor Proteins / chemistry; Repressor Proteins / genetics; Repressor Proteins / metabolism; Siphoviridae / genetics; Siphoviridae / physiology; Viral Proteins / chemistry; Viral Proteins / genetics; Viral Proteins / metabolism; Viral Regulatory and Accessory Proteins
TL;DR: It is demonstrated that a number of different P335 phages, lytic for L. lactis NCK203, have a common operator region which can be targeted by a truncated derivative of a dysfunctional CI repressor. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Overexpression of peptidases in Lactococcus and evaluation of their release from leaky cells

JOURNAL OF DAIRY SCIENCE, 85(10), 2438–2450.

By: T. Tuler n, M. Callanan n & T. Klaenhammer n

author keywords: leaky Lactococcus; peptidase
MeSH headings : Aminopeptidases / genetics; Aminopeptidases / metabolism; Bacterial Proteins; Cloning, Molecular; Coumarins / metabolism; DNA Restriction Enzymes; Escherichia coli / genetics; Gene Expression; Genetic Vectors; Glutamyl Aminopeptidase; Lactococcus / enzymology; Lactococcus / genetics; Lactococcus lactis / enzymology; Lactococcus lactis / genetics; Metalloendopeptidases / genetics; Metalloendopeptidases / metabolism; Oligopeptides / metabolism; Peptide Hydrolases / genetics; Peptide Hydrolases / metabolism; Plasmids / genetics; Promoter Regions, Genetic; Substrate Specificity; Transformation, Bacterial
TL;DR: Results showed different levels of enzymatic overexpression associated with the cellular fraction and negligible amounts of activity present within the supernatant fraction of leaky lactococcal peptidases investigated in this study. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2001 patent

Acid-inducible promoters for gene expression

Washington, DC: U.S. Patent and Trademark Office.

By: M. Kullen & T. Klaenhammer

Source: NC State University Libraries
Added: August 6, 2018

2001 journal article

Analysis of the genetic switch and replication region of a P335-type bacteriophage with an obligate lytic lifestyle on Lactococcus lactis

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 67(3), 1128–1139.

By: S. Madsen*, D. Mills*, G. Djordjevic*, H. Israelsen* & T. Klaenhammer n

MeSH headings : Amino Acid Sequence; Base Sequence; DNA-Binding Proteins; Gene Expression Regulation, Viral; Genes, Switch; Genes, Viral; Lactococcus lactis / virology; Lysogeny / genetics; Molecular Sequence Data; Mucoproteins / genetics; Mucoproteins / metabolism; Promoter Regions, Genetic / genetics; Replication Origin / genetics; Repressor Proteins / genetics; Repressor Proteins / metabolism; Sequence Analysis, DNA; Siphoviridae / genetics; Siphoviridae / physiology; Viral Proteins; Viral Regulatory and Accessory Proteins
TL;DR: Several regions showing extensive DNA and protein homologies to different temperate phages ofLactococcus, Lactobacillus, andStreptococcus were also discovered, indicating the likely exchange of DNA cassettes through horizontal gene transfer in the dynamic ecological environment of dairy fermentations. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2001 journal article

Efficient system for directed integration into the Lactobacillus acidophilus and Lactobacillus gasseri chromosomes via homologous recombination

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 67(9), 4361–4364.

By: W. Russell* & T. Klaenhammer n

MeSH headings : Biotechnology / methods; Chromosomes, Bacterial / genetics; Genetic Vectors; Glucuronidase / genetics; Lactobacillus / genetics; Lactobacillus acidophilus / genetics; Plasmids / genetics; Recombination, Genetic / genetics; beta-Galactosidase / genetics
TL;DR: An efficient method is described for the generation of site-specific chromosomal integrations in Lactobacillus acidophilus and LactOBacillus gasseri using the lactococcal pORI system and relies on the simultaneous use of two plasmids. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2001 journal article

Identification and cloning of gusA, encoding a new beta-glucuronidase from Lactobacillus gasseri ADH

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 67(3), 1253–1261.

By: W. Russell & T. Klaenhammer n

MeSH headings : Amino Acid Sequence; Cloning, Molecular; DNA, Bacterial / genetics; Escherichia coli / enzymology; Escherichia coli / genetics; Glucuronidase / chemistry; Glucuronidase / genetics; Glucuronidase / metabolism; Lactobacillus / enzymology; Lactobacillus / genetics; Molecular Sequence Data; Open Reading Frames; Sequence Analysis, DNA
TL;DR: This is the first report of a β-glucuronidase gene cloned from a bacterial source other than Escherichia coli, and preliminary characterization of the GusA protein from crude cell extracts revealed that the enzyme was active across an acidic pH range and a broad temperature range. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2001 journal article

Leaky Lactococcus cultures that externalize enzymes and antigens independently of culture lysis and secretion and export pathways

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 67(1), 251–259.

By: S. Walker n & T. Klaenhammer n

MeSH headings : Antigens, Bacterial / metabolism; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Bacteriophages / enzymology; Bacteriophages / genetics; Bacteriophages / physiology; Culture Media; Genetic Engineering; Lactococcus lactis / enzymology; Lactococcus lactis / genetics; Lactococcus lactis / growth & development; Lactococcus lactis / metabolism; Lysogeny; N-Acetylmuramoyl-L-alanine Amidase; Viral Proteins / genetics; Viral Proteins / metabolism; Virus Integration; beta-Galactosidase / metabolism
TL;DR: A novel system that leaks β-galactosidase (β-gal) without a requirement for secretion or export signals was developed in lactococcus lactis by controlled expression of integrated phage holin and lysin cassettes, pointing toward the development of bacterial strains able to efficiently release relevant proteins and enzymes outside the cell in the absence of known secretion and export signals. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2001 review

The scientific basis of Lactobacillus acidophilus NCFM functionality as a probiotic

[Review of ]. JOURNAL OF DAIRY SCIENCE, 84(2), 319–331.

By: M. Sanders n & T. Klaenhammer n

author keywords: Lactobacillus acidophilus NCFM; probiotic; functional food
MeSH headings : Anticarcinogenic Agents; Bacterial Adhesion; Dairy Products / microbiology; Dietary Supplements; Gastrointestinal Agents; Genotype; Humans; Immunity; Lactobacillus acidophilus / classification; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / physiology; Lactose Intolerance; Phenotype; Probiotics / therapeutic use
TL;DR: Lactobacillus acidophilus NCFM is the progenitor of the strain being used for complete chromosome sequencing and therefore will be a cornerstone strain for understanding the relationship between genetics and probiotic functionality. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2000 journal article

An explosive antisense RNA strategy for inhibition of a lactococcal bacteriophage

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 66(1), 310–319.

By: S. Walker n & T. Klaenhammer n

MeSH headings : Bacteriophages / genetics; Bacteriophages / physiology; Cloning, Molecular; Escherichia coli / genetics; Gene Expression Regulation, Viral; Lactococcus / virology; Open Reading Frames / genetics; Plasmids / genetics; Polymerase Chain Reaction; Promoter Regions, Genetic; RNA, Antisense / metabolism; RNA, Messenger / metabolism; Replication Origin; Sequence Analysis, DNA; Transformation, Bacterial; Virus Replication
TL;DR: Growth curves performed in the presence of varying levels of phage φ31 showed that ori31plus anti-ORF 4H offered significant protection to Lactococcus lactis, even at multiplicities of infection of 0.01 and 0.1. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2000 journal article

Genetic analysis of chromosomal regions of Lactococcus lactis acquired by recombinant lytic phages

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 66(3), 895–903.

By: E. Durmaz* & T. Klaenhammer n

MeSH headings : Bacteriolysis; Bacteriophages / genetics; Chromosome Mapping; Chromosomes, Bacterial / genetics; DNA, Viral / genetics; Genetic Variation; Lactococcus lactis / genetics; Lactococcus lactis / virology; Mitomycin; Plasmids / genetics; Proviruses; Recombination, Genetic; Replication Origin; Sequence Analysis, DNA; Virus Activation; Virus Integration
TL;DR: It is demonstrated that recombinant phages can acquire DNA cassettes from different regions of the chromosome in order to overcome Abi defenses and alter the types and diversity of new phages that appear during phage-host interactions. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2000 journal article

Genetic modification of intestinal lactobacilli and bifidobacteria

Current Issues in Molecular Biology, 2(2), 41.

By: M. Kullen n & T. Klaenhammer*

TL;DR: This review will describe the tools and strategies currently available for the genetic manipulation of lactobacilli and bifidobacteria, and the ramifications and opportunities that may arise as a result of the genetic manipulations of probiotic lacto-bacteria. (via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018

2000 article

Probiotic bacteria: Today and tomorrow

Klaenhammer, T. R. (2000, February). JOURNAL OF NUTRITION, Vol. 130, pp. 415S–416S.

By: T. Klaenhammer n

author keywords: probiotics; lactic acid bacteria; lactobacillus; bifidobacterium; dairy
MeSH headings : Animals; Dairy Products / microbiology; Humans; Nutritional Physiological Phenomena; Probiotics / metabolism; Probiotics / therapeutic use
TL;DR: An overview of the key issues raised during this symposium is provided, from which there is substantial evidence for positive effects on human health and general well-being. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2000 journal article

Use of the DNA sequence of variable regions of the 16S rRNA gene for rapid and accurate identification of bacteria in the Lactobacillus acidophilus complex

JOURNAL OF APPLIED MICROBIOLOGY, 89(3), 511–516.

By: M. Kullen n, R. Sanozky-Dawes n, D. Crowell n & T. Klaenhammer n

MeSH headings : Base Sequence; DNA, Bacterial / analysis; DNA, Bacterial / genetics; Humans; Immunoglobulin Variable Region; Lactobacillus acidophilus / genetics; Molecular Sequence Data; Polymerase Chain Reaction; RNA, Ribosomal, 16S / analysis; Sequence Alignment; Sequence Homology, Nucleic Acid
TL;DR: A rapid and definitive DNA sequence‐based identification system for unknown isolates of the Lact. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

1999 journal article

Colicin V can be produced by lactic acid bacteria

LETTERS IN APPLIED MICROBIOLOGY, 29(1), 37–41.

By: J. McCormick n, T. Klaenhammer n & M. Stiles*

MeSH headings : Bacteriocins / genetics; Bacteriocins / metabolism; Colicins / biosynthesis; Colicins / genetics; Colicins / pharmacology; Escherichia coli / drug effects; Escherichia coli / genetics; Escherichia coli / growth & development; Genes, Bacterial; Lactobacillaceae / genetics; Lactobacillaceae / growth & development; Lactobacillaceae / metabolism; Lactococcus / genetics; Lactococcus / growth & development; Lactococcus / metabolism; Plasmids; Protein Sorting Signals / genetics; Recombinant Proteins / biosynthesis; Transformation, Bacterial
TL;DR: This system may serve as a model for the heterologous expression of other small bacteriocins active against Gram‐negative bacteria and other antibacterial peptides from lactic acid bacteria. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

1999 journal article

Identification of the pH-inducible, proton-translocating F1F0- ATPase (atpBEFHAGDC) operon of Lactobacillus acidophilus by differential display: gene structure, cloning and characterization

Molecular Microbiology, 33(6), 1152–1161.

By: M. Kullen n & T. Klaenhammer n

MeSH headings : Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA Primers / genetics; DNA, Bacterial / genetics; Genes, Bacterial; Hydrogen-Ion Concentration; Lactobacillus acidophilus / enzymology; Lactobacillus acidophilus / genetics; Molecular Sequence Data; Operon; Proton-Translocating ATPases / genetics; Proton-Translocating ATPases / metabolism; RNA, Bacterial / genetics; RNA, Bacterial / metabolism; RNA, Messenger / genetics; RNA, Messenger / metabolism
TL;DR: The primary structure and genetic organization indicated that the H+‐ATPase of L. acidophilus is a typical F1F0‐type ATPase, which may function in the ATP‐dependent extrusion of protons and maintenance of cytoplasmic pH. (via Semantic Scholar)