Vincent L C Chiang

Works (118)

Updated: April 5th, 2024 09:50

2024 journal article

Ectopic Expression of <i>PtrLBD39</i> Retarded Primary and Secondary Growth in <i>Populus trichocarpa</i>

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 25(4).

By: J. Yu *, B. Gao*, D. Li *, S. Li *, V. Chiang n, W. Li *, C. Zhou *

author keywords: PtrLBD39; wood formation; secondary cell wall; primary growth; Populus trichocarpa
TL;DR: This study indicates that, following ectopic expression in P. trichocarpa, PtrLBD39 functions as a repressor influencing both primary and secondary growth, causing growth inhibition, decreased fibrocyte secondary cell wall thickness, and reduced wood production. (via Semantic Scholar)
Source: Web Of Science
Added: March 11, 2024

2023 article

Cell-type-specific PtrWOX4a and PtrVCS2 form a regulatory nexus with a histone modification system for stem cambium development in Populus trichocarpa

Dai, X., Zhai, R., Lin, J., Wang, Z., Meng, D., Li, M., … Li, W. (2023, January 9). NATURE PLANTS.

By: X. Dai *, R. Zhai *, J. Lin *, Z. Wang *, D. Meng*, M. Li *, Y. Mao*, B. Gao* ...

MeSH headings : Cambium / genetics; Populus / genetics; Epigenesis, Genetic; Histone Code; Wood; Gene Expression Regulation, Plant
TL;DR: A regulatory system in which transcription factor and histone acetylation functions are coordinated for maintaining normal stem vascular cambium development for wood formation in Populus trichocarpa is revealed. (via Semantic Scholar)
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2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Source: Web Of Science
Added: January 23, 2023

2023 article

Exceptionally high genetic variance of the doubled haploid (DH) population of poplar

Liu, C., Wang, S., Liu, Y., Wang, M., Fan, E., Liu, C., … Qu, G. (2023, May 4). JOURNAL OF FORESTRY RESEARCH, Vol. 5.

By: C. Liu *, S. Wang *, Y. Liu *, M. Wang *, E. Fan *, C. Liu *, S. Zhang*, C. Yang* ...

author keywords: Populus; Anther culture; Doubled haploid; Genetic variance; Flow cytometry
TL;DR: This study provides an effective protocol for poplar haploid induction based on an anther culture method and shows that some excellent homozygous lines have the potential to be model plants in genetic and breeding studies. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: May 30, 2023

2023 journal article

Multiplex CRISPR editing of wood for sustainable fiber production

SCIENCE, 381(6654), 216-+.

By: D. Sulis  n, X. Jiang  n, C. Yang n, B. Marques  n, M. Matthews n, Z. Miller n, K. Lan n , C. Cofre-Vega  n ...

TL;DR: This work demonstrates that genome editing can be harnessed for breeding more efficient trees, which will provide timely opportunities for sustainable forestry and a more efficient bioeconomy. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 9, 2023

2022 article

Dimerization of PtrMYB074 and PtrWRKY19 mediates transcriptional activation of PtrbHLH186 for secondary xylem development in Populus trichocarpa

Liu, H., Gao, J., Sun, J., Li, S., Zhang, B., Wang, Z., … Li, W. (2022, March 3). NEW PHYTOLOGIST, Vol. 5.

By: H. Liu *, J. Gao *, J. Sun *, S. Li *, B. Zhang *, Z. Wang *, C. Zhou *, D. Sulis  n ...

author keywords: Populus trichocarpa; protein-protein interaction; secondary xylem development; transcription factor; wood formation
MeSH headings : Cell Wall / metabolism; Dimerization; Gene Expression Regulation, Plant; Plant Proteins / genetics; Plant Proteins / metabolism; Populus; Transcriptional Activation; Wood; Xylem / metabolism
TL;DR: Knowledge of the PtrMYB074‐PtrWRKY19‐PtrbHLH186 regulation may help design genetic controls of optimal growth and wood formation to maximize beneficial wood properties while minimizing negative effects on growth. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: March 21, 2022

2022 journal article

The Manchurian Walnut Genome: Insights into Juglone and Lipid Biosynthesis

GIGASCIENCE, 11.

By: X. Li *, K. Cai *, Q. Zhang *, X. Pei*, S. Chen *, L. Jiang *, Z. Han *, M. Zhao * ...

author keywords: Juglans mandshurica; PacBio SMART; Hi-C; HiFi; genome assembly; comparative genomics; juglone; lipid
MeSH headings : Chromosomes; Genome; Juglans / genetics; Lipids; Naphthoquinones
TL;DR: Combining the high-quality genome, transcriptome, and metabolomics data, a gene-to-metabolite network was constructed and identified 566 core and conserved differentially expressed genes, which may be involved in juglone biosynthesis. (via Semantic Scholar)
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Source: Web Of Science
Added: September 19, 2022

2021 review

An Overview of the Practices and Management Methods for Enhancing Seed Production in Conifer Plantations for Commercial Use

[Review of ]. HORTICULTURAE, 7(8).

By: Y. Li *, X. Li *, M. Zhao *, Z. Pang, J. Wei*, M. Tigabu *, V. Chiang n, H. Sederoff n , R. Sederoff n, X. Zhao *

author keywords: conifers; flowering; seed production; pollination; phytohormones; tree management; nutrient fertilization
TL;DR: The review revealed that flowering and seed yields in conifers are affected by a variety of factors, such as pollen, temperature, light, water availability, nutrients, etc., and a number of management techniques, including topping off, pruning, fertilization, hormone treatment, supplementary pollination, etc. has been developed for improving cone yields. (via Semantic Scholar)
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13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: September 7, 2021

2021 article

CRISPR-Cas9 editing of CAFFEOYL SHIKIMATE ESTERASE 1 and 2 shows their importance and partial redundancy in lignification in Populus tremula x P. alba

Vries, L., Brouckaert, M., Chanoca, A., Kim, H., Regner, M. R., Timokhin, V. I., … Boerjan, W. (2021, August 6). PLANT BIOTECHNOLOGY JOURNAL, Vol. 11.

By: L. Vries *, M. Brouckaert *, A. Chanoca *, H. Kim *, M. Regner*, V. Timokhin *, Y. Sun *, B. De Meester * ...

author keywords: poplar; CRISPR-Cas9; CSE; lignin; phenylpropanoids; metabolic engineering
MeSH headings : CRISPR-Cas Systems / genetics; Carboxylesterase; Gene Expression Regulation, Plant; Lignin / metabolism; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Populus / genetics; Populus / metabolism
TL;DR: The results suggest an alternative path within the phenylpropanoid pathway at the level of the hydroxycinnamoyl‐shikimates, and show that CSE is a promising target to improve plants for the biorefinery. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 23, 2021

2021 review

Cooperative Regulation of Flavonoid and Lignin Biosynthesis in Plants

[Review of ]. CRITICAL REVIEWS IN PLANT SCIENCES, 40(2), 109–126.

By: S. Zhang *, J. Yang *, H. Li *, V. Chiang n & Y. Fu *

author keywords: Cooperative regulation; transcription factor; flavonoid; lignin; phenylpropanoid pathway
TL;DR: Recent progress on function and mechanism of transcription regulatory networks sharing certain specific regulators, such as transcription factors, mediators and microRNAs are summarized and a simplified discussion for the different co-regulation networks involved with flavonoid and lignin biosynthesis is proposed. (via Semantic Scholar)
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Source: Web Of Science
Added: April 19, 2021

2021 journal article

Enzyme Complexes of Ptr4CL and PtrHCT Modulate Co-enzyme A Ligation of Hydroxycinnamic Acids for Monolignol Biosynthesis in Populus trichocarpa

FRONTIERS IN PLANT SCIENCE, 12.

By: C. Lin n, Y. Sun *, J. Song n, H. Chen n, R. Shi n, C. Yang n, J. Liu  n, S. Tunlaya-Anukit n ...

author keywords: protein interaction; monolignol biosynthesis; wood formation; Populus trichocarpa; BiFC; metabolic flux
TL;DR: It is proposed that Ptr4CL/PtrHCT complexes modulate the metabolic flux of CoA ligation for monolignol biosynthesis during wood formation in P. trichocarpa. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: November 8, 2021

2021 review

Histone Acetylation Changes in Plant Response to Drought Stress

[Review of ]. GENES, 12(9).

By: S. Li *, X. He *, Y. Gao *, C. Zhou *, V. Chiang n & W. Li*

author keywords: drought stress; histone acetylation; histone acetyltransferases (HATs); histone deacetylases (HDACs)
MeSH headings : Acetylation; Adaptation, Biological / genetics; Adaptation, Biological / physiology; Dehydration / genetics; Dehydration / metabolism; Dehydration / physiopathology; Droughts; Histone Acetyltransferases / metabolism; Histone Deacetylases / metabolism; Histones / metabolism; Plant Development / genetics; Plant Development / physiology; Protein Processing, Post-Translational
TL;DR: This review summarizes histone acetylation changes in plant response to drought stress, and reviews the functions of HATs and HDACs in drought response and resistance. (via Semantic Scholar)
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6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: October 4, 2021

2021 review

MYB-Mediated Regulation of Anthocyanin Biosynthesis

[Review of ]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22(6).

By: H. Yan *, X. Pei*, H. Zhang *, X. Li*, X. Zhang *, M. Zhao *, V. Chiang n, R. Sederoff n, X. Zhao *

author keywords: MYB; transcription factor; anthocyanin; positive regulation; negative regulation; environment; MBW complexes
MeSH headings : Anthocyanins / biosynthesis; Gene Expression Regulation, Plant; Gene Regulatory Networks; Phylogeny; Plant Proteins / chemistry; Plant Proteins / metabolism; Transcription Factors / chemistry; Transcription Factors / metabolism
TL;DR: Some prospects for MYB-TF to modulate the comprehensive regulation of anthocyanins are put forward to provide a more relevant basis for further research in this field, and to guide the directed genetic modification of anthcyanins for the improvement of crops for food quality, nutrition and human health. (via Semantic Scholar)
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Source: Web Of Science
Added: May 24, 2021

2021 journal article

Molecular and Metabolic Insights into Anthocyanin Biosynthesis for Leaf Color Change in Chokecherry (Padus virginiana)

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22(19).

By: X. Li *, Y. Li *, M. Zhao *, Y. Hu *, F. Meng *, X. Song *, M. Tigabu *, V. Chiang n ...

author keywords: Padus virginiana; leaf color; anthocyanin biosynthesis; transcriptomics; metabolomic
MeSH headings : Anthocyanins / biosynthesis; Biosynthetic Pathways; Chlorophyll / biosynthesis; Color; Computational Biology / methods; Gene Expression Profiling; Gene Expression Regulation, Plant; Metabolome; Metabolomics / methods; Pigmentation / genetics; Plant Leaves / genetics; Plant Leaves / metabolism; Prunus / physiology; Transcriptome
TL;DR: In PRL, the expression of the genes encoding for UGFT and BZ1 enzymes was substantially higher than in leaves of other colors and may be related with the purple-red color change, which may facilitate genetic modification or selection for further improvement in ornamental qualities of P. virginiana. (via Semantic Scholar)
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15. Life on Land (OpenAlex)
Source: Web Of Science
Added: October 26, 2021

2021 journal article

Transcriptional reprogramming of xylem cell wall biosynthesis in tension wood

PLANT PHYSIOLOGY, 186(1), 250–269.

By: B. Liu *, J. Liu *, J. Yu *, Z. Wang *, Y. Sun *, S. Li *, Y. Lin*, V. Chiang n, W. Li*, J. Wang n 

MeSH headings : Cell Wall / metabolism; Gene Expression Regulation, Plant; Populus / anatomy & histology; Populus / genetics; Transcription, Genetic; Wood / genetics; Wood / metabolism; Xylem / metabolism
TL;DR: A transcriptional regulatory network is established in which PtrHSFB3-1 and PtrMYB092 directly activate 8 and 11 monolignol genes, respectively in P. trichocarpa, to further uncover how metabolism, growth, and stress adaptation are coordinately regulated in trees. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: May 27, 2021

2021 journal article

p A PtrLBD39-mediated transcriptional network regulates tension wood formation in Populus trichocarpa

PLANT COMMUNICATIONS, 3(1).

By: J. Yu *, C. Zhou *, D. Li *, S. Li *, Y. Lin*, J. Wang n , V. Chiang n, W. Li*

author keywords: tension wood; cellulose; lignin; transcriptional regulatory network; transcriptomics; chromatin immunoprecipitation sequencing
MeSH headings : Biomechanical Phenomena; Gene Expression Regulation, Plant / physiology; Gene Regulatory Networks / physiology; Genes, Plant / genetics; Genes, Plant / physiology; Laser Capture Microdissection; Populus / genetics; Populus / physiology; Wood / genetics; Wood / physiology; Xylem
TL;DR: This work suggests that P trLBD39 and PtrLBD22 specifically control TW formation by mediating a TW-specific TRN in Populus by directly regulating 26 novel TW-responsive transcription factor genes. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: January 31, 2022

2020 journal article

A multiscale model of lignin biosynthesis for predicting bioenergy traits in Populus trichocarpa

COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL, 19, 168–182.

By: M. Matthews *, J. Wang n , R. Sederoff n, V. Chiang n & C. Williams n 

author keywords: Lignin biosynthesis; Multiscale modeling; Cross-regulatory influences; Random forests
Sources: Web Of Science, NC State University Libraries, ORCID
Added: September 20, 2021

2020 journal article

Fungal Pretreatment and Enzymatic Hydrolysis of Genetically-modified Populus trichocarpa

BIORESOURCES, 15(3), 6488–6505.

By: C. Edmunds  n, P. Peralta n, R. Sharma-Shivappa n, S. Kelley n , V. Chiang n, Z. Miller n, R. Giles *, R. Sykes * ...

author keywords: Transgenic poplar; Fungal treatment; Ceriporiopsis; Pretreatment; Enzymatic hydrolysis; Lignin
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6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 24, 2020

2020 review

Microbial Interactions Within Multiple-Strain Biological Control Agents Impact Soil-Borne Plant Disease

[Review of ]. FRONTIERS IN MICROBIOLOGY, 11.

By: B. Niu *, W. Wang*, Z. Yuan*, R. Sederoff n, H. Sederoff n , V. Chiang n, R. Borriss *

Contributors: B. Niu *, W. Wang*, Z. Yuan*, R. Sederoff n, H. Sederoff n , V. Chiang n, R. Borriss *

author keywords: microbial interaction; biological control agents; soil-borne disease; consortia; microbiome and community
TL;DR: The current state of the use of single strain and multi-strain biological control consortia in the management of soil-borne diseases and potential mechanisms used by microbial components to improve the disease suppressing efficacy are summarized. (via Semantic Scholar)
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2. Zero Hunger (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: November 16, 2020

2020 journal article

Qu-2, a robust poplar suspension cell line for molecular biology

JOURNAL OF FORESTRY RESEARCH, 32(2), 733–740.

By: C. Liu *, K. Li *, M. Wang *, E. Fan *, C. Yang*, J. Wang *, P. Fu*, X. Ge* ...

Contributors: C. Liu *, K. Li *, M. Wang *, E. Fan *, C. Yang*, J. Wang *, P. Fu*, X. Ge* ...

author keywords: Qu-2 cell line; Suspension cell; Poplar; Protoplast isolation; Transient transformation
TL;DR: A hormone autotrophic poplar suspension cell line from a hybrid of Populus alba × P. berolinensis ‘Yinzhong’, named Qu-2, which has the great potential to be an excellent model cell line in tree molecular biological research, ranging from physiology to gene function. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: January 4, 2021

2020 journal article

Use of the lignocellulose-degrading bacterium Caldicellulosiruptor bescii to assess recalcitrance and conversion of wild-type and transgenic poplar

BIOTECHNOLOGY FOR BIOFUELS, 13(1).

By: C. Straub n, R. Bing n , J. Wang n , V. Chiang n, M. Adams * & R. Kelly n 

author keywords: Caldicellulosiruptor; Extreme thermophiles; Lignocellulose; Biofuel; Poplar
TL;DR: Utilizing C. bescii as a screening assay for recalcitrance, poplar lines with down-regulation of coumarate 3-hydroxylase 3 (C3H3) resulted in the highest degrees of solubilization and conversion by C. Bescii, suggesting that excellent digestibility can be achieved without compromising fitness of the tree. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: April 6, 2020

2019 journal article

A novel synthetic-genetic-array-based yeast one-hybrid system for high discovery rate and short processing time

GENOME RESEARCH, 29(8), 1343–1351.

By: C. Yeh *, Z. Wang *, F. Miao*, H. Ma *, C. Kao *, T. Hsu*, J. Yu *, E. Hung* ...

Contributors: C. Yeh *, Z. Wang *, F. Miao*, H. Ma *, C. Kao *, T. Hsu*, J. Yu *, E. Hung* ...

MeSH headings : Animals; DNA / genetics; DNA / metabolism; Gene Expression Regulation; Gene Regulatory Networks; Genetic Markers; Humans; Meiosis; Microarray Analysis / instrumentation; Microarray Analysis / methods; Plasmids / chemistry; Plasmids / metabolism; Ploidies; Populus / cytology; Protein Binding; Protoplasts / cytology; Protoplasts / metabolism; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism; Time Factors; Transcription Factors / genetics; Transcription Factors / metabolism; Two-Hybrid System Techniques
TL;DR: A high-throughput meiosis-directed yeast one-hybrid system using the Magic Markers of the synthetic genetic array analysis that has a transcription factor–DNA interaction discovery rate twice as high as the conventional diploid-mating approach and a processing time nearly one-tenth of the haploid-transformation method. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: September 16, 2019

2019 journal article

ANATOMICAL, PHYSICAL, AND MECHANICAL PROPERTIES OF TRANSGENIC LOBLOLLY PINE (PINUS TAEDA L.) MODIFIED FOR INCREASED DENSITY

WOOD AND FIBER SCIENCE, 51(2), 173–182.

By: Z. Miller  n, P. Peralta n, P. Mitchell n, S. Kelley* , V. Chiang n, L. Pearson, W. Rottmann, M. Cunningham, I. Peszlen n 

author keywords: Density; specific gravity; quantitative wood anatomy; tracheid; MOE; MOR; transgenic; Pinus taeda L.
TL;DR: Transgenic trees from sets OX41 and OX55, modified for increased density using two variants of the same HAP5 gene, exhibited higher mechanical properties with smaller stem diameter and tracheid lumen diameter than their set of control trees. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
13. Climate Action (Web of Science)
15. Life on Land (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 12, 2019

2019 journal article

Anatomy and Chemistry of Populus trichocarpa with Genetically Modified Lignin Content

BIORESOURCES, 14(3), 5729–5746.

By: Z. Miller  n, P. Peralta n, P. Mitchell n, V. Chiang n, S. Kelley n , C. Edmunds  n, I. Peszlen n 

author keywords: Populus trichocarpa; Lignin content; Syringyl-to-guaiacyl (S/G) ratio; Stem diameter; CAD; C3H; C4H
TL;DR: Changes in lignin structure from modifications to PtrCAD resulted in reductions to the number of vessels, increases in vessel and fiber diameters, and had no consistent impact on stem diameter. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: July 22, 2019

2019 personal communication

Certification for gene-edited forests

Strauss, S. H., Boerjan, W., Chiang, V., Costanza, A., Coleman, H., Davis, J. M., … Valenzuela, S. (2019, August 23).

By: S. Strauss *, W. Boerjan *, V. Chiang n, A. Costanza*, H. Coleman *, J. Davis *, M. Lu *, S. Mansfield * ...

MeSH headings : Certification; Climate Change; Consumer Behavior; Forests; Gene Editing; Pest Control, Biological; Trees / genetics; Wood
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2. Zero Hunger (Web of Science)
Source: Web Of Science
Added: September 16, 2019

2019 review

Enzyme-Enzyme Interactions in Monolignol Biosynthesis

[Review of ]. FRONTIERS IN PLANT SCIENCE, 9.

By: J. Wang n , B. Liu *, Y. Sun *, V. Chiang n & R. Sederoff n

Contributors: J. Wang n , B. Liu *, Y. Sun *, V. Chiang n & R. Sederoff n

author keywords: monolignol biosynthesis; protein-protein interaction; lignin; enzyme kinetics; BiFC; co-immunoprecipitation
TL;DR: Support for interactions comes from new studies of enzyme activity, co-immunoprecipitation, chemical crosslinking, bimolecular fluorescence complementation, yeast 2-hybrid functional screening, and cell type–specific gene expression based on light amplification by stimulated emission of radiation capture microdissection. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: January 28, 2019

2019 journal article

Quantitative fermentation of unpretreated transgenic poplar by Caldicellulosiruptor bescii

NATURE COMMUNICATIONS, 10(1).

By: C. Straub n, P. Khatibi n, J. Wang n , J. Conway n, A. Williams-Rhaesa *, I. Peszlen n , V. Chiang n, M. Adams *, R. Kelly n 

Contributors: C. Straub n, P. Khatibi n, J. Wang n , J. Conway n, A. Williams-Rhaesa *, I. Peszlen n , V. Chiang n, M. Adams *, R. Kelly n 

MeSH headings : Biomass; Cellulose / metabolism; Clostridiales / genetics; Clostridiales / metabolism; Ethanol / metabolism; Fermentation; Industrial Microbiology; Lignin / metabolism; Metabolic Engineering; Plants, Genetically Modified / chemistry; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Polysaccharides / metabolism; Populus / chemistry; Populus / genetics; Populus / metabolism
TL;DR: The authors pair transgenic lignin modified poplar lines with engineered Caldicellusiruptor bescii to achieve biomass solubilization and ethanol conversion without pretreatment and the nearly quantitative microbial conversion of the carbohydrate content of unpretreated transgen lignocellulosic biomass bodes well for full utilization of renewable biomass feedstocks. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 26, 2019

2019 journal article

Thermo-mechanical Behavior of Genetically Modified Populus trichocarpa

BIORESOURCES, 14(2), 4760–4773.

By: C. Edmunds  n, I. Peszlen n , V. Chiang n, S. Kelley n , Z. Miller n, M. Davis *, E. Gjersing*, P. Peralta n

author keywords: Genetically modified poplar; Lignin; Viscoelastic properties; Glass transition temperature; Dynamic mechanical analysis; Cinnamyl alcohol dehydrogenase (CAD); Cinnamate 3-hydroxylase (C3H); Cinnamate 4-hydroxylase (C4H)
Sources: Web Of Science, NC State University Libraries
Added: April 20, 2020

2018 journal article

Altered Lignin Content and Composition in Transgenic Populus trichocarpa Results in a Decrease of Modulus of Elasticity

BIORESOURCES, 13(4), 7698–7708.

By: Z. Miller, P. Peralta*, P. Mitchell*, V. Chiang*, C. Edmunds & I. Peszlen* 

author keywords: CAD; C3H; C4H; Lignin; Glucose; Xylose; Modulus of Elasticity
TL;DR: Significant changes in chemical composition, created by genetic manipulations of the lignin biosynthetic pathway, affect the mechanical properties of young small-diameter transgenic trees. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: January 14, 2019

2018 journal article

Assessing the impact of the 4CL enzyme complex on the robustness of monolignol biosynthesis using metabolic pathway analysis

PLOS ONE, 13(3), e0193896.

By: P. Naik n, J. Wang n , R. Sederoff n, V. Chiang n, C. Williams n  & J. Ducoste n 

Contributors: P. Naik n, J. Wang n , R. Sederoff n, V. Chiang n, C. Williams n  & J. Ducoste n 

Ed(s): D. Cullen

MeSH headings : Biosynthetic Pathways; Homeostasis; Lignin / biosynthesis; Metabolic Networks and Pathways; Monte Carlo Method; Plant Proteins / metabolism; Populus / enzymology; Populus / metabolism
TL;DR: Results from robustness and stability analyses suggest that the monolignol biosynthetic pathway is resilient to mild perturbations in the presence of the Ptr4CL3-Ptr4CL5 complex, which increased the stability of the pathway by 22%. (via Semantic Scholar)
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7. Affordable and Clean Energy (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018

2018 journal article

CAD1 and CCR2 protein complex formation in monolignol biosynthesis in Populus trichocarpa

NEW PHYTOLOGIST, 222(1), 244–260.

By: X. Yan *, J. Liu  n, H. Kim *, B. Liu *, X. Huang *, Z. Yang n, Y. Lin*, H. Chen n ...

Contributors: X. Yan *, J. Liu  n, H. Kim *, B. Liu *, X. Huang *, Z. Yang n, Y. Lin *, H. Chen n ...

author keywords: co-immunoprecipitation; enzyme activity; monolignol biosynthetic pathway; nuclear magnetic resonance (NMR); Populus trichocarpa; stem-differentiating xylem protein
MeSH headings : Aldehyde Oxidoreductases / genetics; Aldehyde Oxidoreductases / metabolism; Down-Regulation / genetics; Gene Expression Regulation, Plant; Lignin / metabolism; Magnetic Resonance Spectroscopy; Plant Proteins / genetics; Plant Proteins / metabolism; Plants, Genetically Modified; Populus / genetics; Populus / metabolism; RNA Interference; Recombinant Proteins / metabolism; Xylem / metabolism
TL;DR: Evidence is provided for the formation of PtrCAD1/PtrCCR2 protein complexes in monolignol biosynthesis in planta, and biomolecular fluorescence complementation and pull-down/co-immunoprecipitation experiments supported a hypothesis of P Tr trichocarpa heterodimer formation. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: December 17, 2018

2018 journal article

Flux modeling for monolignol biosynthesis

Current Opinion in Biotechnology, 56, 187–192.

By: J. Wang n , M. Matthews  n, P. Naik n, C. Williams n , J. Ducoste n , R. Sederoff  n, V. Chiang n

Contributors: J. Wang n , M. Matthews  n, P. Naik n, C. Williams n , J. Ducoste n , R. Sederoff  n, V. Chiang n

MeSH headings : Biosynthetic Pathways; Kinetics; Lignin / biosynthesis; Metabolic Engineering; Metabolic Flux Analysis; Models, Biological
TL;DR: This review summarizes the recent progress in flux modeling and its application to lignin engineering for improved plant development and utilization. (via Semantic Scholar)
Sources: Crossref, ORCID, NC State University Libraries
Added: April 24, 2019

2018 journal article

Improving wood properties for wood utilization through multi-omics integration in lignin biosynthesis

Nature Communications, 9(1), 1579.

By: J. Wang n , M. Matthews  n, C. Williams n , R. Shi n, C. Yang  n, S. Tunlaya-Anukit  n, H. Chen n, Q. Li * ...

Contributors: J. Wang n , M. Matthews  n, C. Williams n , R. Shi n, C. Yang  n, S. Tunlaya-Anukit  n, H. Chen n, Q. Li * ...

MeSH headings : Gene Expression Regulation, Plant; Lignin / biosynthesis; Lignin / genetics; Plants, Genetically Modified / genetics; Populus / genetics; Populus / metabolism; Transcriptome / genetics; Trees / genetics; Trees / metabolism; Wood / chemistry; Wood / physiology; Xylem / metabolism
TL;DR: A multi-omics quantitative integrative analysis of lignin biosynthesis can advance the strategic engineering of wood for timber, pulp, and biofuels and may lead to greater understanding of other pathways for improved growth and adaptation. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018

2017 journal article

Characterization and enzymatic hydrolysis of wood from transgenic Pinus taeda engineered with syringyl lignin or reduced lignin content

CELLULOSE, 24(4), 1901–1914.

By: C. Edmunds  n, P. Peralta n, S. Kelley n , V. Chiang n, R. Sharma-Shivappa n, M. Davis *, A. Harman-Ware *, R. Sykes * ...

author keywords: Softwood; Lignin modification; Lignocellulose; Pyrolysis-molecular beam mass spectroscopy; Solid-state NMR
TL;DR: No apparent benefit was observed by S lignin incorporation at the concentrations reported in this study, which highlights the potential for softwood cell wall properties to be improved for bioenergy/biochemical applications. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2017 journal article

Field-Grown Transgenic Hybrid Poplar with Modified Lignin Biosynthesis to Improve Enzymatic Saccharification Efficiency

ACS SUSTAINABLE CHEMISTRY & ENGINEERING, 5(3), 2407–2414.

By: Z. Xiang *, S. Sen, D. Min*, D. Savithri*, F. Lu *, H. Jameel*, V. Chiang*, H. Chang*

author keywords: Transgenic; 4CL gene; Enzymatic saccharification; Hybrid poplar; Field-grown; Lignin
Source: Web Of Science
Added: August 6, 2018

2017 journal article

Reciprocal cross-regulation of VND and SND multigene TF families for wood formation in Populus trichocarpa

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 114(45), E9722–E9729.

By: Y. Lin  n, H. Chen n, Q. Li *, W. Li n, J. Wang n , R. Shi n, S. Tunlaya-Anukit n, P. Shuai n ...

Contributors: Y. Lin  n, H. Chen n, Q. Li *, W. Li n, J. Wang n , R. Shi n, S. Tunlaya-Anukit n, P. Shuai n ...

author keywords: reciprocal cross-regulation; NAC transcription factors; alternative splicing; wood formation; Populus trichocarpa
MeSH headings : Alternative Splicing; Cell Wall / genetics; Cell Wall / metabolism; Cloning, Molecular; DNA, Plant; Gene Expression Regulation, Plant / genetics; Gene Regulatory Networks; Genes, Plant; Homeostasis; Multigene Family; Nuclear Proteins; Plant Leaves / genetics; Plant Leaves / metabolism; Plant Proteins / genetics; Plant Proteins / metabolism; Plant Shoots / genetics; Plant Shoots / metabolism; Populus / genetics; Populus / metabolism; Recombinant Proteins / genetics; Transcription Factors / genetics; Transcription Factors / metabolism; Transcriptional Activation / genetics; Transcriptome; Wood / genetics; Wood / growth & development; Xylem / genetics; Xylem / growth & development
TL;DR: The results indicate that the splice variants from the PtrVND6 and PtrSND1 family may exert reciprocal cross-regulation for complete transcriptional regulation of these two families in wood formation, suggesting a general mechanism among NAC domain proteins and likely other transcription factors. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2017 journal article

Takayoshi Higuchi (1927–2017)

Journal of Wood Chemistry and Technology, 37(5), 405–406.

By: H. Chang, R. Sederoff, V. Chiang* & T. Umezawa

Source: Crossref
Added: August 28, 2020

2017 journal article

Tissue and cell-type co-expression networks of transcription factors and wood component genes in Populus trichocarpa

PLANTA, 245(5), 927–938.

By: R. Shi n, J. Wang n , Y. Lin n, Q. Li *, Y. Sun *, H. Chen n, R. Sederoff n, V. Chiang*

Contributors: R. Shi n, J. Wang n , Y. Lin n, Q. Li *, Y. Sun *, H. Chen n, R. Sederoff n, V. Chiang*

author keywords: Wood formation; Transcriptome; Fiber cells; Vessel elements; Cell wall biosynthesis; Co-expression network
MeSH headings : Cell Wall / metabolism; Cellulose / metabolism; Cluster Analysis; Gene Expression Regulation, Plant; Lignin / metabolism; Molecular Sequence Annotation; Organ Specificity; Phloem / genetics; Phloem / growth & development; Plant Leaves / genetics; Plant Leaves / growth & development; Plant Proteins / genetics; Populus / genetics; Populus / growth & development; Sequence Analysis, RNA; Transcription Factors / genetics; Transcriptome; Wood / genetics; Wood / growth & development; Xylem / genetics; Xylem / growth & development
TL;DR: The co-expression networks revealed here suggest a well-structured transcriptional homeostasis for cell wall component biosynthesis during wood formation. (via Semantic Scholar)
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2. Zero Hunger (OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2016 journal article

A cell wall-bound anionic peroxidase, PtrPO21, is involved in lignin polymerization in Populus trichocarpa

Tree Genetics & Genomes, 12(2).

By: C. Lin  n, Q. Li *, S. Tunlaya-Anukit *, R. Shi n, Y. Sun *, J. Wang n , J. Liu  n, P. Loziuk n ...

Contributors: C. Lin  n, Q. Li *, S. Tunlaya-Anukit *, R. Shi n, Y. Sun *, J. Wang n , J. Liu  n, P. Loziuk n ...

author keywords: Lignin polymerization; Populus trichocarpa; Lignin peroxidase; LC-MS/MS; Lignin systems biology
TL;DR: Differentially expressed gene (DEG) analysis of PtrPO21 downregulated transgenics identified a significant overexpression of PtPrx35, suggesting a compensatory effect within the peroxidase family. (via Semantic Scholar)
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15. Life on Land (OpenAlex)
Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: August 6, 2018

2016 journal article

Bottom-up GGM algorithm for constructing multilayered hierarchical gene regulatory networks that govern biological pathways or processes

BMC BIOINFORMATICS, 17.

By: S. Kumari *, W. Deng *, C. Gunasekara*, V. Chiang n, H. Chen *, H. Ma *, X. Davis n, H. Wei *

author keywords: Multilayered gene regulatory network; Pathway; Microarray or RNA-seq data
MeSH headings : Algorithms; Arabidopsis / genetics; Arabidopsis / metabolism; Arabidopsis Proteins / genetics; Computational Biology / methods; Gene Regulatory Networks; Humans; Metabolic Networks and Pathways / genetics; Signal Transduction / genetics; Transcriptome
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Source: Web Of Science
Added: August 6, 2018

2015 journal article

4-Coumaroyl and Caffeoyl Shikimic Acids Inhibit 4-Coumaric Acid: Coenzyme A Ligases and Modulate Metabolic Flux for 3-Hydroxylation in Monolignol Biosynthesis of Populus trichocarpa

MOLECULAR PLANT, 8(1), 176–187.

By: C. Lin n, J. Wang n , Q. Li *, H. Chen n, J. Liu  n, P. Loziuk n, J. Song  n, C. Williams n  ...

Contributors: C. Lin n, J. Wang n , Q. Li *, H. Chen n, J. Liu  n, P. Loziuk n, J. Song  n, C. Williams n  ...

author keywords: monolignol biosynthesis; Populus trichocarpa; metabolic flux; reaction and inhibition kinetics; LC-MS/MS; 4-coumaroyl and caffeoyl shikimic acids
MeSH headings : Acyl Coenzyme A / metabolism; Coenzyme A Ligases / metabolism; Coumaric Acids / metabolism; Coumaric Acids / pharmacology; Hydroxylation / drug effects; Plant Proteins / metabolism; Populus / drug effects; Populus / enzymology; Populus / metabolism; Propionates; Shikimic Acid / pharmacology
TL;DR: 4-coumaroyl and caffeoyl shikimic acids are inhibitors of Ptr4CL3 and Ptr 4CL5, which can reduce lignin content in a number of plant species and play significant regulatory roles when these inhibitors accumulate. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2015 journal article

Elucidation of Xylem-Specific Transcription Factors and Absolute Quantification of Enzymes Regulating Cellulose Biosynthesis in Populus trichocarpa

JOURNAL OF PROTEOME RESEARCH, 14(10), 4158–4168.

By: P. Loziuk n, J. Parker  n, W. Li n, C. Lin n, J. Wang n , Q. Li *, R. Sederoff n, V. Chiang n, D. Muddiman n 

Contributors: P. Loziuk n, J. Parker  n, W. Li n, C. Lin n, J. Wang n , Q. Li *, R. Sederoff n, V. Chiang n, D. Muddiman n 

author keywords: shotgun discovery proteomics; absolute quantification; PC-IDMS; cellulose biosynthesis; transcription factor; targeted mass spectrometiry; SRM
MeSH headings : Carbohydrate Metabolism; Cellulose / biosynthesis; Cellulose / genetics; Chromatography, Liquid; Gene Expression Regulation, Plant; Molecular Sequence Annotation; Plant Proteins / genetics; Plant Proteins / isolation & purification; Plant Proteins / metabolism; Populus / genetics; Populus / metabolism; Proteome / genetics; Proteome / isolation & purification; Proteome / metabolism; Proteomics; Tandem Mass Spectrometry; Transcription Factors / genetics; Transcription Factors / isolation & purification; Transcription Factors / metabolism; Wood / chemistry; Wood / metabolism; Xylem / genetics; Xylem / metabolism
TL;DR: The study described herein sought to identify the proteins directly involved in cellulose biosynthesis during wood formation in Populus trichocarpa along with known xylem-specific transcription factors involved in regulating these key proteins. (via Semantic Scholar)
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9. Industry, Innovation and Infrastructure (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2015 journal article

Phosphorylation is an on/off switch for 5-hydroxyconiferaldehyde O-methyltransferase activity in poplar monolignol biosynthesis

Proceedings of the National Academy of Sciences, 112(27), 8481–8486.

By: J. Wang n , L. Chuang n, P. Loziuk n, H. Chen n, Y. Lin n, R. Shi n, G. Qu *, D. Muddiman n , R. Sederoff n, V. Chiang n

Contributors: J. Wang n , L. Chuang n, P. Loziuk n, H. Chen n, Y. Lin n, R. Shi n, G. Qu *, D. Muddiman n , R. Sederoff n, V. Chiang n

author keywords: AldOMT; COMT; lignin; phosphoproteomics; phosphoregulation
MeSH headings : Acrolein / analogs & derivatives; Acrolein / metabolism; Amino Acid Sequence; Binding Sites / genetics; Biocatalysis; Catechols / metabolism; Chromatography, Liquid; Electrophoresis, Polyacrylamide Gel; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Isoenzymes / genetics; Isoenzymes / metabolism; Lignin / biosynthesis; Methyltransferases / genetics; Methyltransferases / metabolism; Microscopy, Confocal; Molecular Sequence Data; Mutation; Phosphoproteins / metabolism; Phosphorylation; Plant Proteins / genetics; Plant Proteins / metabolism; Populus / enzymology; Populus / genetics; Populus / metabolism; Proteomics / methods; Protoplasts / enzymology; Protoplasts / metabolism; Sequence Homology, Amino Acid; Tandem Mass Spectrometry
TL;DR: It is shown that reversible monophosphorylation at Ser123 or Ser125 acts as an on/off switch for the activity of 5-hydroxyconiferaldehyde O-methyltransferase 2 (PtrAldOMT2), an enzyme central to monolignol biosynthesis for lignification in stem-differentiating xylem of Populus trichocarpa. (via Semantic Scholar)
Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: August 6, 2018

2015 journal article

Wood characteristics and enzymatic saccharification efficiency of field-grown transgenic black cottonwood with altered lignin content and structure

CELLULOSE, 22(1), 683–693.

By: Z. Xiang n, S. Sen  n, A. Roy  n, D. Min n, D. Savithri n, H. Jameel n, V. Chiang n, H. Chang n

author keywords: Black cotton wood; Enzymatic saccharification; Field-grown; Lignin; Pretreatment; S/V ratio; Sugar yield; Transgenic
Source: Web Of Science
Added: August 6, 2018

2014 journal article

A new O-methyltransferase for monolignol synthesis in Carthamus tinctorius

Plant Biotechnology, 31(5), 545–553.

By: T. Nakatsubo*, S. Ragamustari *, T. Hattori*, E. Ono *, M. Yamamura *, L. Li n, V. Chiang n, T. Umezawa *

author keywords: O-Methyltransferase; Carthamus tinctorius; lignin; monolignol; flavonoid
TL;DR: The data strongly suggest that along with CtCAldOMT1, CtAAOMT is involved in biosynthesis of syringyl lignin in Carthamus tinctorius. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2014 journal article

A robust chromatin immunoprecipitation protocol for studying transcription factor-DNA interactions and histone modifications in wood-forming tissue

NATURE PROTOCOLS, 9(9), 2180–2193.

By: W. Li n, Y. Lin n, Q. Li n, R. Shi n, C. Lin  n, H. Chen n, L. Chuang n, G. Qu *, R. Sederoff n, V. Chiang n

MeSH headings : Base Sequence; Chromatin Immunoprecipitation / methods; DNA / metabolism; DNA Fragmentation; Histones / metabolism; Immunoprecipitation; Molecular Sequence Data; Populus / cytology; Populus / metabolism; Sequence Analysis, DNA; Transcription Factors / metabolism; Wood / cytology; Xylem / genetics; Xylem / metabolism
TL;DR: This protocol is the only working ChIP system for wood-forming tissue and uses stem-differentiating xylem to identify genome-wide specific TF-DNA interactions during wood formation and histone modifications associated with regulation of wood formation. (via Semantic Scholar)
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2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2014 journal article

A simple improved-throughput xylem protoplast system for studying wood formation

NATURE PROTOCOLS, 9(9), 2194–2205.

By: Y. Lin n, W. Li n, H. Chen n, Q. Li n, Y. Sun *, R. Shi n, C. Lin n, J. Wang n  ...

Contributors: Y. Lin n, W. Li n, H. Chen n, Q. Li n, Y. Sun *, R. Shi n, C. Lin n, J. Wang n  ...

MeSH headings : Cell Wall / metabolism; Gene Expression Regulation, Plant / genetics; Gene Regulatory Networks / genetics; Green Fluorescent Proteins; Populus / cytology; Populus / growth & development; Protoplasts / metabolism; Transfection / methods; Wood / cytology; Wood / growth & development; Xylem / chemistry
TL;DR: A protocol for the isolation and transfection of protoplasts from wood-forming tissue, the stem-differentiating xylem (SDX), in the model woody plant Populus trichocarpa is presented. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2014 journal article

Establishing ion ratio thresholds based on absolute peak area for absolute protein quantification using protein cleavage isotope dilution mass spectrometry

ANALYST, 139(21), 5439–5450.

By: P. Loziuk n, R. Sederoff n, V. Chiang n & D. Muddiman n 

MeSH headings : Chromatography, Liquid; Isotopes / chemistry; Proteins / analysis; Tandem Mass Spectrometry / methods
TL;DR: It is suggested that evaluation of the absolute ion abundance for any given transition is necessary in order to effectively implement RA thresholds, and these RA values should be assessed as close as possible to the time at which data is collected for quantification. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2014 journal article

Improved Protocol for Alkaline Nitrobenzene Oxidation of Woody and Non-Woody Biomass

Journal of Wood Chemistry and Technology, 35(1), 52–61.

By: D. Min n, Z. Xiang n, J. Liu  n, H. Jameel n, V. Chiang n, Y. Jin *, H. Chang n

author keywords: Nitrobenzene oxidation; survivival factors; lignin model compounds; Sr/V and B/V ratios
Sources: Crossref, Web Of Science
Added: August 6, 2018

2014 journal article

Phenolic Compounds and Expression of 4CL Genes in Silver Birch Clones and Pt4CL1a Lines

PLOS ONE, 9(12).

By: S. Sutela *, T. Hahl*, H. Tiimonen, T. Aronen, T. Ylioja, T. Laakso, P. Saranpaa, V. Chiang n, R. Julkunen-Tiitto *, H. Haggman *

MeSH headings : Betula / enzymology; Betula / genetics; Betula / metabolism; Biomechanical Phenomena; Cloning, Molecular; Coenzyme A Ligases / deficiency; Coenzyme A Ligases / genetics; Gene Expression Regulation, Plant; Phenols / chemistry; Phenols / metabolism; Plant Leaves / genetics; Plants, Genetically Modified; Populus / genetics; RNA, Messenger / genetics; RNA, Messenger / metabolism; Solubility
TL;DR: The results indicate that by reducing the Bp4CL1 transcript levels lignin composition could be modified, as an overall trend the wounding decreased the flavonoid content in silver birches and increased the content of soluble condensed tannins. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Pop's Pipes: poplar gene expression data analysis pipelines

TREE GENETICS & GENOMES, 10(4), 1093–1101.

By: X. Li *, C. Gunasekara n, Y. Guo*, H. Zhang *, L. Lei *, S. Tunlaya-Anukit n, V. Busov *, V. Chiang n, H. Wei *

author keywords: Poplar; Microarray; RNA-seq data; Differentially expressed genes; Pathway enrichment analysis; Gene ontology enrichment analysis; Protein domain enrichment analysis; Pipeline; Gene ontology tree
TL;DR: Pop’s Pipes can be used to identify differentially expressed genes between one or multiple paired tissues, time points, or treatment-vs-control conditions in a single in silico analysis and enables scrutiny to create spatiotemporal models and hypotheses to understand how poplar develops and functions. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2014 article

Populus trichocarpa

AGROBACTERIUM PROTOCOLS, VOLUME 2, THIRD EDITION, Vol. 1224, pp. 357–363.

By: Q. Li*, T. Yeh *, C. Yang*, J. Song *, Z. Chen*, R. Sederoff*, V. Chiang*

author keywords: Agrobacterium tumefaciens; Genetic transformation; Populus trichocarpa; Transgenic trees
MeSH headings : Agrobacterium tumefaciens / genetics; Agrobacterium tumefaciens / growth & development; Coculture Techniques; Genetic Engineering / methods; Plant Roots / growth & development; Plant Shoots / growth & development; Populus / genetics; Populus / growth & development; Populus / physiology; Regeneration; Transformation, Genetic
TL;DR: A modified step-by-step protocol for Agrobacterium-mediated transformation of stem explants is described here and the health of the tissue explants and the time of cocultivation are among the critical steps in the protocol for successful transformation. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2014 journal article

Systems Biology of Lignin Biosynthesis in Populus trichocarpa: Heteromeric 4-Coumaric Acid:Coenzyme A Ligase Protein Complex Formation, Regulation, and Numerical Modeling

The Plant Cell, 26(3), 876–893.

By: H. Chen n, J. Song  n, J. Wang n , Y. Lin  n, J. Ducoste n , C. Shuford n, J. Liu  n, Q. Li n ...

Contributors: H. Chen n, J. Song  n, J. Wang n , Y. Lin  n, J. Ducoste n , C. Shuford n, J. Liu  n, Q. Li n ...

MeSH headings : Coenzyme A Ligases / genetics; Coenzyme A Ligases / metabolism; Coumaric Acids / metabolism; Immunoprecipitation; Lignin / biosynthesis; Mass Spectrometry; Models, Biological; Populus / metabolism; Propionates; RNA, Messenger / genetics; Substrate Specificity; Systems Biology
TL;DR: This work shows that 4CL, an enzyme in monolignol biosynthesis, is found as a heterotetrameric complex of two isoforms in Populus trichocarpa, where the two 4-coumaric acid:CoA ligase (4CL) isoforms, 4CL3 and 4CL5, interact in vivo and in vitro to form a heterosymmetric protein complex. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries, Crossref
Added: August 6, 2018

2014 journal article

Vibrational sum-frequency-generation (SFG) spectroscopy study of the structural assembly of cellulose microfibrils in reaction woods

CELLULOSE, 21(4), 2219–2231.

By: K. Kafle*, R. Shi n, C. Lee *, A. Mittal *, Y. Park *, Y. Sun n, S. Park n , V. Chiang n, S. Kim *

author keywords: Reaction wood; Tension wood; Compression wood; Cellulose microfibril assembly; Sum; frequency; generation spectroscopy; X-ray; diffraction
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2013 journal article

A lignan O-methyltransferase catalyzing the regioselective methylation of matairesinol in Carthamus tinctorius

PLANT BIOTECHNOLOGY, 30(2), 97–109.

By: T. Umezawa *, S. Ragamustari *, T. Nakatsubo*, S. Wada*, L. Li n, M. Yamamura *, N. Sakakibara *, T. Hattori*, S. Suzuki *, V. Chiang n

author keywords: Carthamus tinctorius; lignan; matairesinol; O-methyltransferase; regioselective methylation
TL;DR: Quantitative real-time polymerase chain reaction analysis demonstrated that expression of the CtMROMT gene was synchronized with the O-methyltransferase (OMT) activity profile and arctigenin accumulation in the plant, and demonstrated that CtMRomT is a novel plant OMT for lignan methylation. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

High-level gene expression in differentiating xylem of tobacco driven by a 2.0 kb Poplar COMT2 promoter and a 4 x 35S enhancer

PLANT BIOTECHNOLOGY, 30(2), 191–198.

By: E. Liu n, S. Peng, Q. Li n, Y. Sun *, V. Chiang n & R. Sederoff n

author keywords: PtrCOMT2 promoter; xylem-specific; 4 x 35S enhancer; woody plants; genetic engineering
TL;DR: While expression of some of the enhancer-plus-promoter constructs increased expression, there was a loss of specificity in the PtrCOMT2 promoter. (via Semantic Scholar)
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3. Good Health and Well-being (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Monolignol Pathway 4-Coumaric Acid: Coenzyme A Ligases in Populus trichocarpa: Novel Specificity, Metabolic Regulation, and Simulation of Coenzyme A Ligation Fluxes

PLANT PHYSIOLOGY, 161(3), 1501–1516.

By: H. Chen n, J. Song  n, C. Williams n , C. Shuford n, J. Liu  n, J. Wang n , Q. Li n, R. Shi n ...

Contributors: H. Chen n, J. Song n, C. Williams n , C. Shuford n, J. Liu  n, J. Wang n , Q. Li n, R. Shi n ...

MeSH headings : Allosteric Regulation / drug effects; Binding Sites; Biosynthetic Pathways / drug effects; Blotting, Western; Caffeic Acids / pharmacology; Coenzyme A / metabolism; Coenzyme A Ligases / antagonists & inhibitors; Coenzyme A Ligases / metabolism; Computer Simulation; Coumaric Acids / chemistry; Coumaric Acids / metabolism; Coumaric Acids / pharmacology; Kinetics; Lignin / biosynthesis; Lignin / chemistry; Phenylpropionates / metabolism; Phosphoproteins / metabolism; Phosphorylation / drug effects; Plant Extracts; Populus / drug effects; Populus / enzymology; Propionates; Proteomics; Recombinant Fusion Proteins / metabolism; Sequence Homology, Amino Acid; Substrate Specificity / drug effects; Xylem / drug effects; Xylem / metabolism
TL;DR: Evidence is presented of two monolignol 4CLs, Ptr4CL3 and P tr4CL5, in Populus trichocarpa that act to mitigate the inhibition by caffeic acid to maintain a proper ligation flux and coenzyme A flux with novel specificity. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2013 journal article

Ptr-miR397a is a negative regulator of laccase genes affecting lignin content in Populus trichocarpa

Proceedings of the National Academy of Sciences, 110(26), 10848–10853.

By: S. Lu *, Q. Li n, H. Wei *, M. Chang*, S. Tunlaya-Anukit  n, H. Kim *, J. Liu  n, J. Song * ...

MeSH headings : Base Sequence; Down-Regulation / genetics; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Gene Regulatory Networks; Genes, Plant; Laccase / antagonists & inhibitors; Laccase / genetics; Lignin / antagonists & inhibitors; Lignin / chemistry; Lignin / metabolism; MicroRNAs / genetics; MicroRNAs / metabolism; Phylogeny; Plant Proteins / genetics; Populus / enzymology; Populus / genetics; RNA, Plant / genetics; RNA, Plant / metabolism
TL;DR: A hierarchical genetic regulatory network (GRN) built by a bottom-up graphic Gaussian model algorithm provides additional support for a role of ptr-miR397a as a negative regulator of laccases for lignin biosynthesis in wood formation. (via Semantic Scholar)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

2013 journal article

Regulation of phenylalanine ammonia-lyase (PAL) gene family in wood forming tissue of Populus trichocarpa

Planta, 238(3), 487–497.

By: R. Shi n, C. Shuford n, J. Wang n , Y. Sun n, Z. Yang n, H. Chen n, S. Tunlaya-Anukit n, Q. Li n ...

Contributors: R. Shi n, C. Shuford n, J. Wang n , Y. Sun n, Z. Yang n, H. Chen n, S. Tunlaya-Anukit n, Q. Li n ...

MeSH headings : Gene Expression Regulation, Plant / physiology; Mass Spectrometry; Phenylalanine Ammonia-Lyase / metabolism; Populus / enzymology; Populus / metabolism
TL;DR: A greater functional significance of subgroup A PtrPALs for stem wood formation is indicated, and the role of gene post-transcriptional regulation is highlighted. (via Semantic Scholar)
Sources: NC State University Libraries, ORCID, NC State University Libraries
Added: August 6, 2018

2013 journal article

SND1 Transcription Factor-Directed Quantitative Functional Hierarchical Genetic Regulatory Network in Wood Formation in Populus trichocarpa

PLANT CELL, 25(11), 4324–4341.

By: Y. Lin n, W. Li n, Y. Sun *, S. Kumari *, H. Wei *, Q. Li n, S. Tunlaya-Anukit n, R. Sederoff n, V. Chiang n

MeSH headings : Algorithms; Cell Wall / genetics; Chromatin Immunoprecipitation; Gene Expression Regulation, Plant; Gene Ontology; Gene Regulatory Networks; High-Throughput Nucleotide Sequencing; Models, Genetic; Plant Proteins / genetics; Plant Proteins / metabolism; Plants, Genetically Modified; Polymerase Chain Reaction / methods; Populus / genetics; Populus / metabolism; Protoplasts; Reproducibility of Results; Transcription Factors / genetics; Transcription Factors / metabolism; Transfection; Wood / genetics; Wood / metabolism; Xylem / cytology; Xylem / genetics
TL;DR: A genome-wide high-throughput system for the discovery and validation of specific transcription factor (TF)–directed hierarchical gene regulatory networks (hGRNs) in wood formation is developed and may be particularly useful to study hGRNs in complex processes in plant species resistant to stable genetic transformation and where mutants are unavailable. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

The Amborella Genome and the Evolution of Flowering Plants

Science, 342(6165), 1456–1457.

By: J. Pacelli, R. Sederoff, V. Chiang & J. Acosta Jaramillo  (4 connected NC State authors and many others, 148 authors in total )

Contributors: C. DePamphilis *, J. Palmer, S. Rounsley*, D. Sankoff, S. Schuster *, J. Ammiraju*, W. Barbazuk *, S. Chamala ...

MeSH headings : Conservation of Natural Resources; Contig Mapping; Evolution, Molecular; Flowers / genetics; Gene Duplication; Genetic Variation; Genome, Plant; Metagenomics / statistics & numerical data; Molecular Sequence Annotation; Multigene Family; New Caledonia; Phylogeny; Sequence Analysis, DNA; Tracheophyta / classification; Tracheophyta / genetics; Tracheophyta / growth & development
TL;DR: Genome structure and phylogenomic analyses indicate that the ancestral Angiosperm was a polyploid with a large constellation of both novel and ancient genes that survived to play key roles in angiosperm biology. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2013 journal article

The elucidation of the lignin structure effect on the cellulase-mediated saccharification by genetic engineering poplars (Populus nigra L. x Populus maximowiczii A.)

BIOMASS & BIOENERGY, 58, 52–57.

By: D. Min n, C. Yang n, R. Shi n, H. Jameel n, V. Chiang n & H. Chang n

author keywords: Transgenic hybrid poplar; Pretreatment; S to V Ratio; Delignification; Enzymatic saccharification
TL;DR: The decrease of the lignin and the hemicelluloses benefited the enzymatic saccharification with or without the pretreatment, and the high S to V ratio retarded the enzyme. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2013 journal article

The influence of lignin-carbohydrate complexes on the cellulase-mediated saccharification II: Transgenic hybrid poplars (Populus nigra L. and Populus maximowiczii A.)

FUEL, 116, 56–62.

By: D. Min n, C. Yang  n, V. Chiang n, H. Jameel n & H. Chang n

author keywords: Hybrid P. trichocarpa; Lignin carbohydrate complexes (LCCs); Enzymatic saccharification; Syringaldehyde/vanillin ratio; 1H-13C NMR
TL;DR: It was found that the samples showed different levels of the three LCC linkages, depending on the lignin content and/or the S/V ratio, demonstrated that the LCCs accounted for the recalcitrance of lignocellulosic biodegradation. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2013 journal article

Understanding the Role of Proteolytic Digestion on Discovery and Targeted Proteomic Measurements Using Liquid Chromatography Tandem Mass Spectrometry and Design of Experiments

JOURNAL OF PROTEOME RESEARCH, 12(12), 5820–5829.

By: P. Loziuk n, J. Wang n , Q. Li n, R. Sederoff n, V. Chiang n & D. Muddiman n 

Contributors: P. Loziuk n, J. Wang n , Q. Li n, R. Sederoff n, V. Chiang n & D. Muddiman n 

author keywords: design of experiments; proteolytic digestion; LC-MS/MS; Populus trichocarpa
MeSH headings : Amino Acid Sequence; Animals; Cattle; Chromatography, Liquid / standards; Factor Analysis, Statistical; Hydrogen-Ion Concentration; Isotope Labeling; Molecular Sequence Annotation; Molecular Sequence Data; Peptide Fragments / chemistry; Peptide Fragments / isolation & purification; Plant Proteins / chemistry; Plant Proteins / isolation & purification; Populus / chemistry; Protein Stability; Proteolysis; Proteomics / methods; Proteomics / standards; Research Design; Swine; Tandem Mass Spectrometry / standards; Trypsin / chemistry; Xylem / chemistry
TL;DR: The tryptic digest optimization described here has eliminated this previously observed peptide decay as well as provided a greater understanding and standardization for a common but critical sample treatment used across the field of proteomics. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2013 journal article

nnThe influence of lignin-carbohydrate complexes on the cellulase-mediated saccharification I: Transgenic black cottonwood (western balsam poplar, California poplar) P-trichocarpa including the xylan down-regulated and the lignin down-regulated lines

FUEL, 119, 207–213.

By: D. Min n, Q. Li n, V. Chiang n, H. Jameel n, H. Chang n & L. Lucia n 

author keywords: Transgenic P. trichocarpa; Lignin-carbohydrate complexes (LCCs); Enzymatic saccharification; Fermentable sugars; H-1-C-13 HSQC NMR
TL;DR: The correlation between the LCCs and enzymatic saccharification nearly conclusively demonstrated that the L CCs accounting for the recalcitrance of lignocellulosic biodegradation. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

A standard reaction condition and a single HPLC separation system are sufficient for estimation of monolignol biosynthetic pathway enzyme activities

PLANTA, 236(3), 879–885.

By: J. Liu  n, R. Shi n, Q. Li n, R. Sederoff n & V. Chiang n

author keywords: Monolignols; Enzyme activity; Populus trichocarpa; Pinus taeda; Angiosperm; Gymnosperm
MeSH headings : Biosynthetic Pathways; Chromatography, High Pressure Liquid; Enzyme Assays; Lignin / biosynthesis; Methyltransferases / metabolism; Mixed Function Oxygenases / metabolism; Oxidoreductases / metabolism; Pinus taeda / enzymology; Plant Proteins / analysis; Plant Stems / enzymology; Populus / enzymology; Substrate Specificity; Xylem / enzymology
TL;DR: This work developed a method using a total protein extract (soluble and microsomal) for the comprehensive and simultaneous analysis of these ten enzyme activities in a single target tissue, stem differentiating xylem (SDX) of Populustrichocarpa. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2012 journal article

Comprehensive Quantification of Monolignol-Pathway Enzymes in Populus trichocarpa by Protein Cleavage Isotope Dilution Mass Spectrometry

Journal of Proteome Research, 11(6), 3390–3404.

By: C. Shuford  n, Q. Li n, Y. Sun n, H. Chen n, J. Wang n , R. Shi n, R. Sederoff n, V. Chiang n, D. Muddiman n 

Contributors: C. Shuford  n, Q. Li n, Y. Sun n, H. Chen n, J. Wang n , R. Shi n, R. Sederoff n, V. Chiang n, D. Muddiman n 

author keywords: quantification; isotope dilution mass spectrometry; selected reaction monitoring; filter-aided sample preparation; monolignol biosynthesis
MeSH headings : Amino Acid Sequence; Biosynthetic Pathways; Isotope Labeling; Molecular Sequence Data; Plant Proteins / chemistry; Plant Proteins / metabolism; Populus / enzymology; Proteome / chemistry; Proteome / metabolism; Reference Standards; Spectrometry, Mass, Electrospray Ionization / standards; Xylem / enzymology
TL;DR: This represents the most comprehensive assay for quantification of proteins regulating monolignol biosynthesis and will lead to a better understanding of lignin formation at a systems level. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, ORCID, Crossref, NC State University Libraries
Added: August 6, 2018

2012 journal article

Functional redundancy of the two 5-hydroxylases in monolignol biosynthesis of Populus trichocarpa: LC-MS/MS based protein quantification and metabolic flux analysis

PLANTA, 236(3), 795–808.

By: J. Wang n , C. Shuford n, Q. Li n, J. Song  n, Y. Lin n, Y. Sun n, H. Chen n, C. Williams n  ...

Contributors: J. Wang n , C. Shuford n, Q. Li n, J. Song  n, Y. Lin n, Y. Sun n, H. Chen n, C. Williams n  ...

author keywords: Lignin; Syringyl lignin; Angiosperm; Kinetics; Subcellular localization
MeSH headings : Cloning, Molecular; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Hydroxylation; Kinetics; Lignin / analysis; Lignin / biosynthesis; Mixed Function Oxygenases / metabolism; Plants, Genetically Modified; Populus / metabolism; Xylem / enzymology; Yeasts / metabolism
TL;DR: The combination of genetic analysis, absolute protein quantitation-based enzyme kinetics, homologous CPR specificity, SNP characterization, and ER localization provides a more rigorous basis for a comprehensive systems understanding of 5-hydroxylation in lignin biosynthesis. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2012 journal article

Peptide Production and Decay Rates Affect the Quantitative Accuracy of Protein Cleavage Isotope Dilution Mass Spectrometry (PC-IDMS)

Molecular & Cellular Proteomics, 11(9), 814–823.

By: C. Shuford n, R. Sederoff  n, V. Chiang n & D. Muddiman n 

MeSH headings : Indicator Dilution Techniques; Isotope Labeling; Mass Spectrometry; Peptide Biosynthesis; Peptides / analysis; Peptides / chemistry; Peptides / metabolism; Populus / enzymology; Populus / metabolism; Proteolysis; Xylem / enzymology
TL;DR: It is concluded that the instability of peptides can often significantly bias the protein quantities measured in protein cleavage isotope dilution mass spectrometry-based assays and suggest peptide stability be made a priority when selecting peptides to use for quantification. (via Semantic Scholar)
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Sources: Crossref, Web Of Science, NC State University Libraries
Added: August 6, 2018

2012 journal article

Splice variant of the SND1 transcription factor is a dominant negative of SND1 members and their regulation in Populus trichocarpa

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109(36), 14699–14704.

By: Q. Li n, Y. Lin n, Y. Sun n, J. Song  n, H. Chen n, X. Zhang *, R. Sederoff n, V. Chiang n

MeSH headings : Blotting, Western; Cloning, Molecular; DNA Primers / genetics; Dimerization; Electrophoresis, Polyacrylamide Gel; Gene Regulatory Networks / genetics; Homeostasis / physiology; Models, Biological; Plasmids / genetics; Populus / genetics; Protein Isoforms / genetics; Protein Transport; Real-Time Polymerase Chain Reaction; Transcription Factors / genetics
TL;DR: A stem-differentiating xylem (SDX)-specific alternative SND1 splice variant, PtrSND1-A2IR, that acts as a dominant negative of S ND1 transcriptional network genes in Populus trichocarpa is discovered and may contribute to transcriptional homeostasis to avoid deleterious effects on xylogenesis and plant growth. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2012 journal article

The Cellulase-Mediated Saccharification on Wood Derived from Transgenic Low-Lignin Lines of Black Cottonwood (Populus trichocarpa)

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 168(4), 947–955.

By: D. Min n, Q. Li n, H. Jameel n, V. Chiang n & H. Chang n

author keywords: Transgenic hardwood; Downregulated lignin; Pretreatment; Enzymatic saccharification
MeSH headings : Cellulase / metabolism; Down-Regulation; Hydrogen-Ion Concentration; Hydrolysis; Lignin / chemistry; Lignin / metabolism; Ozone / chemistry; Plants, Genetically Modified; Polysaccharides / chemistry; Populus / genetics; Populus / metabolism; Sulfuric Acids / chemistry; Wood / chemistry
TL;DR: The results indicated that lignin is the main recalcitrance of biomass degradation, and pretreatment with ozone delignification had lower saccharification efficiency than the other pretreatment methods due to higher xylan content. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2012 conference paper

The effects of transgenic samples on the enzymatic saccharification

Proceeding of the 4th International Conference on Pulping, Papermaking and Biotechnology (ICPPB '12), vols. I and II, 1058–1062.

By: D. Min, Q. Li, C. Yang, H. Chang, V. Chiang & H. Jameel

Source: NC State University Libraries
Added: August 6, 2018

2012 journal article

effect of lignin on enzymatic saccharification of hardwood after green liquor and sulfuric acid pretreatments

BioResources, 7(2), 2272–2283.

By: D. Min*, H. Jameel n, V. Chiang* & H. Chang*

Source: NC State University Libraries
Added: August 6, 2018

2011 journal article

Chemical and Spatial Differentiation of Syringyl and Guaiacyl Lignins in Poplar Wood via Time-of-Flight Secondary Ion Mass Spectrometry

ANALYTICAL CHEMISTRY, 83(18), 7020–7026.

By: C. Zhou  n, Q. Li  n, V. Chiang n, L. Lucia n  & D. Griffis n

MeSH headings : Cell Wall / chemistry; Fullerenes / chemistry; Lignin / analysis; Plant Stems / chemistry; Populus / chemistry; Spectrometry, Mass, Secondary Ion / methods
TL;DR: The spatial distribution of the syringyl (S) and guaiacyl (G) lignins, both over larger regions and within a single cell wall, on poplar ( Populus trichocarpa ) wood cross-sections was determined via time-of-flight secondary ion mass spectrometry (ToF-SIMS). (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 article

Comparison of pretreatment protocols for cellulase-mediated saccharification of wood derived from transgenic low-xylan lines of cottonwood (P. trichocarpa)

Min, D., Li, Q., Jameel, H., Chiang, V., & Chang, H.-min. (2011, August). BIOMASS & BIOENERGY, Vol. 35, pp. 3514–3521.

By: D. Min n, Q. Li  n, H. Jameel n, V. Chiang n & H. Chang n

author keywords: Transgenic tree; Low xylan content; Pretreatment; Enzymatic saccharification; Fermentable sugar
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Source: Web Of Science
Added: August 6, 2018

2011 journal article

Conservation and Diversity of MicroRNA-associated Copper-regulatory Networks in Populus trichocarpa

JOURNAL OF INTEGRATIVE PLANT BIOLOGY, 53(11), 879–891.

By: S. Lu n, C. Yang n & V. Chiang n

author keywords: copper; Cu-response element; microRNA; Populus; SPB domain protein
MeSH headings : Copper / pharmacology; Gene Expression Regulation, Plant / drug effects; Gene Expression Regulation, Plant / genetics; MicroRNAs / metabolism; Plant Proteins / genetics; Plant Proteins / metabolism; Populus / genetics; Populus / metabolism; Promoter Regions, Genetic / genetics
TL;DR: It is reported that Cu-responsive miRNAs in Populus trichocarpa include not only conserved miR397, miR398 and miR408, but also Populus-specific miR1444, suggesting the conservation and diversity of Cu- Response elements in plants. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Down-regulation of glycosyltransferase 8D genes in Populus trichocarpa caused reduced mechanical strength and xylan content in wood

TREE PHYSIOLOGY, 31(2), 226–236.

By: Q. Li n, D. Min n, J. Wang n , I. Peszlen n , L. Horvath n, B. Horvath n, Y. Nishimura n, H. Jameel n, H. Chang n, V. Chiang n

Contributors: Q. Li n, D. Min n, J. Wang  n, I. Peszlen n , L. Horvath n, B. Horvath n, Y. Nishimura n, H. Jameel n, H. Chang n, V. Chiang n

author keywords: glycosyltransferase; Populus trichocarpa; wood formation; xylan
MeSH headings : Arabidopsis / genetics; Arabidopsis / metabolism; Cell Wall / metabolism; Down-Regulation; Elastic Modulus; Gene Expression Regulation, Plant; Genes, Plant; Genetic Variation; Glycosyltransferases / genetics; Glycosyltransferases / metabolism; Plant Stems / chemistry; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Populus / genetics; Populus / metabolism; Stress, Mechanical; Transcription Factors; Wood / analysis; Xylans / biosynthesis; Xylans / genetics
TL;DR: Results suggest that PtrGT8Ds play key roles in xylan biosynthesis in wood, and may be a more important factor than lignin affecting the stiffness and fracture strength of wood. (via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2011 journal article

Effects of lignin-modified Populus tremuloides on soil organic carbon

JOURNAL OF PLANT NUTRITION AND SOIL SCIENCE, 174(5), 818–826.

By: R. Roque-Rivera*, A. Talhelm *, D. Johnson *, V. Chiang n & K. Pregitzer*

author keywords: C-13; carbon storage; soil C formation; transgenic aspen; lignin modifications
TL;DR: The effects of lignin modification on SOC are driven by changes in plant growth, and differences in biomass between genetically modified lines of aspen resulted in differences in the accrual of as pen-derived SOC and total mesocosm C. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Membrane protein complexes catalyze both 4-and 3-hydroxylation of cinnamic acid derivatives in monolignol biosynthesis

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 108(52), 21253–21258.

By: H. Chen*, Q. Li*, C. Shuford n, J. Liu, D. Muddiman n , R. Sederoff*, V. Chiang*

MeSH headings : Carboxylic Ester Hydrolases / chemistry; Carboxylic Ester Hydrolases / metabolism; Chromatography, Liquid; Coumaric Acids; DNA Primers / genetics; Hydroxylation; Immunoprecipitation; Kinetics; Lignin / biosynthesis; Mass Spectrometry; Membrane Proteins / chemistry; Membrane Proteins / metabolism; Microscopy, Confocal; Molecular Structure; Multiprotein Complexes / chemistry; Multiprotein Complexes / metabolism; Phenols; Phenylpropionates; Plasmids / genetics; Populus / metabolism; Propionates; Trans-Cinnamate 4-Monooxygenase / chemistry; Trans-Cinnamate 4-Monooxygenase / metabolism; Xylem / metabolism; Yeasts
TL;DR: Affinity purification-quantitative mass spectrometry, bimolecular fluorescence complementation, chemical cross-linking, and reciprocal coimmunoprecipitation provide further evidence for functional involvement of membrane protein complexes in monolignol biosynthesis. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

MicroRNAs in trees

PLANT MOLECULAR BIOLOGY, 80(1), 37–53.

By: Y. Sun n, R. Shi n, X. Zhang  n, V. Chiang n & R. Sederoff n

author keywords: MicroRNA; Woody plant; Gene regulation; Plant development; Plant stress response; Artificial microRNA
MeSH headings : Gene Expression Regulation, Plant; MicroRNAs / genetics; MicroRNAs / metabolism; Models, Genetic; Phylogeny; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; RNA, Messenger / genetics; RNA, Messenger / metabolism; RNA, Plant / genetics; RNA, Plant / metabolism; Trees / classification; Trees / genetics; Trees / metabolism
TL;DR: The purpose of this review is to summarize the current status of information on mi RNAs in trees and to guide future studies on the role of miRNAs in the biology of woody perennials and to illustrate their utility in directed genetic modification of trees. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Raman-spectroscopy-based noninvasive microanalysis of native lignin structure

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 402(2), 983–987.

By: P. Perera *, M. Schmidt *, V. Chiang n, P. Schuck * & P. Adams *

author keywords: Lignin; Entropy minimization; Chemometrics; Cell wall; Monolignol; Raman
MeSH headings : Arabidopsis / chemistry; Lignin / analysis; Lignin / chemistry; Molecular Structure; Spectrum Analysis, Raman / methods
TL;DR: A new robust, noninvasive, Raman microspectroscopic method is introduced to analyze the structure of native lignin and found similar distributions of S and G monolignols in xylary fiber cells and interfascicular cells. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2010 journal article

Lignin and Biomass: A Negative Correlation for Wood Formation and Lignin Content in Trees

PLANT PHYSIOLOGY, 154(2), 555–561.

By: E. Novaes  n, M. Kirst  n, V. Chiang n, H. Winter-Sederoff & R. Sederoff n

Contributors: E. Novaes  n, M. Kirst  n, V. Chiang n, H. Winter-Sederoff & R. Sederoff n

MeSH headings : Biomass; Gene Expression Regulation, Plant; Lignin / biosynthesis; Lignin / chemistry; Transcription Factors / metabolism; Trees / genetics; Trees / growth & development; Trees / metabolism; Wood / genetics; Wood / growth & development; Wood / metabolism
TL;DR: Studies in populations of forest tree hybrids have shown a negative correlation of biomass growth and lignin content, implying that selection for biomass growth may be highly regulated. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2010 journal article

Specific down-regulation of PAL genes by artificial microRNAs in Populus trichocarpa

PLANTA, 232(6), 1281–1288.

By: R. Shi n, C. Yang n, S. Lu n, R. Sederoff n & V. Chiang n

author keywords: Artificial microRNA; Phenylalanine ammonia-lyase; Populus trichocarpa transformation; Down-regulation; PolyA tailing-based real time RT-PCR; Quantitation
MeSH headings : Base Sequence; DNA Primers; Down-Regulation; Genes, Plant; MicroRNAs / physiology; Mutation; Populus / genetics; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Nucleic Acid
TL;DR: This paper describes the specific down-regulation of multiple genes in the phenylalanine ammonia-lyase (PAL) gene family of Populus trichocarpa using amiRNA sequences incorporated in a ptc-MIR408 miRNA-producing precursor, revealing differential regulation within the gene family. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Adenylation of plant miRNAs

NUCLEIC ACIDS RESEARCH, 37(6), 1878–1885.

By: S. Lu n, Y. Sun n & V. Chiang n

MeSH headings : Adenosine Monophosphate / analysis; Genetic Variation; MicroRNAs / chemistry; MicroRNAs / metabolism; Populus / genetics; RNA 3' End Processing; RNA Stability; RNA, Plant / chemistry; RNA, Plant / metabolism; Sequence Analysis, RNA
TL;DR: It is shown that a significant portion of the isolated miRNAs contains, at the 3′-end, one or a few post-transcriptionally added adenylic acid residues, which are distinct in length from the polyadenylate tail added to other plant RNAs for exosome-mediated degradation. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2009 review

An update on the nomenclature for the cellulose synthase genes in Populus

[Review of ]. TRENDS IN PLANT SCIENCE, 14(5), 248–254.

By: M. Kumar *, S. Thammannagowda*, V. Bulone *, V. Chiang n, K. Han *, C. Joshi*, S. Mansfield *, E. Mellerowicz * ...

MeSH headings : Glucosyltransferases / genetics; Phylogeny; Plant Proteins / classification; Populus / enzymology; Terminology as Topic
TL;DR: A new phylogeny-based CesA nomenclature is proposed that aligns the Populus CesA gene family with the established Arabidopsis thaliana CesA family structure. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Characterization of the tissue-specific expression of phenylalanine ammonia-lyase gene promoter from loblolly pine (Pinus taeda) in Nicotiana tabacum

PLANT CELL REPORTS, 28(9), 1309–1317.

By: Y. Osakabe *, K. Osakabe * & V. Chiang n

author keywords: PtaPAL; Lignin; Pinus taeda; AC element
MeSH headings : Base Sequence; DNA, Plant / genetics; Gene Expression Regulation, Plant; Genomic Library; Molecular Sequence Data; Phenylalanine Ammonia-Lyase / genetics; Pinus taeda / genetics; Plant Proteins / genetics; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Promoter Regions, Genetic; Tobacco / genetics; Tobacco / metabolism; Xylem / metabolism
TL;DR: The results suggested that AC elements of PtaPAL have multiple functions in the expression under the various developmental stages and stress conditions in the transgenic tobacco. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Isolation of 4-coumarate Co-A ligase gene promoter from loblolly pine (Pinus taeda) and characterization of tissue-specific activity in transgenic tobacco

PLANT PHYSIOLOGY AND BIOCHEMISTRY, 47(11-12), 1031–1036.

By: Y. Osakabe *, K. Osakabe * & V. Chiang n

author keywords: Pta4CL alpha; Lignin; Pinus taeda; AC element
MeSH headings : Adaptation, Physiological / genetics; Base Sequence; Coenzyme A Ligases / genetics; Coenzyme A Ligases / metabolism; DNA, Plant; Gene Expression; Gene Expression Regulation, Plant; Gene Library; Genes, Plant; Molecular Sequence Data; Nuclear Proteins / metabolism; Pinus taeda / enzymology; Pinus taeda / genetics; Plant Stems / cytology; Plant Stems / genetics; Plants, Genetically Modified / genetics; Promoter Regions, Genetic / physiology; Sequence Homology, Nucleic Acid; Tobacco / genetics; Tobacco / metabolism; Xylem / enzymology; Xylem / genetics; Xylem / growth & development
TL;DR: Promoter activity of a phenylpropanoid biosynthetic gene encoding 4-coumarate Co-A ligase (4CL), Pta4Clalpha, from Pinus taeda was characterized and the AC elements appeared to have individual roles during xylem development that are activated in a coordinated manner in response to stress in transgenic tobacco. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Label-free in situ imaging of lignification in the cell wall of low lignin transgenic Populus trichocarpa

PLANTA, 230(3), 589–597.

By: M. Schmidt*, A. Schwartzberg *, P. Perera *, A. Weber-Bargioni *, A. Carroll *, P. Sarkar *, E. Bosneaga*, J. Urban * ...

author keywords: Populus trichocarpa (black cottonwood); Cell wall; Lignin; Microspectroscopy; Raman imaging; Transgenic
MeSH headings : Cell Wall / metabolism; Lignin / metabolism; Plants, Genetically Modified / cytology; Plants, Genetically Modified / metabolism; Populus / cytology; Populus / metabolism; Spectrum Analysis, Raman
TL;DR: Lignification in wild type and lignin-reduced 4CL transgenic Populus trichocarpa stem wood with spatial resolution that is sub-μm is compared, suggesting that transgenic approach may help overcome cell wall recalcitrance to wood saccharification. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Phenolic compounds in ectomycorrhizal interaction of lignin modified silver birch

BMC PLANT BIOLOGY, 9.

By: S. Sutela *, K. Niemi*, J. Edesi *, T. Laakso, P. Saranpaa, J. Vuosku*, R. Makela*, H. Tiimonen ...

MeSH headings : Betula / genetics; Betula / metabolism; Betula / microbiology; Gene Expression Regulation, Plant; Lignin / biosynthesis; Methyltransferases / genetics; Methyltransferases / metabolism; Mycorrhizae / physiology; Phenols / metabolism; Plant Roots / genetics; Plant Roots / metabolism; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Populus / genetics; RNA, Plant / genetics; Sequence Analysis, DNA; Symbiosis; Tannins / biosynthesis
TL;DR: The down-regulation of BpCOMT in the 35S-PtCOMT lines caused a reduction in the syringyl/guaiacyl ratio of lignin, but no significant effect was seen in the composition or quantity of phenolic compounds that would have been caused by the expression of PtCOMT under the35S or UbB1 promoter. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2009 journal article

Profiling of phenylpropanoid monomers in developing xylem tissue of transgenic aspen (Populus tremuloides)

JOURNAL OF WOOD SCIENCE, 56(1), 71–76.

By: S. Suzuki n, N. Sakakibara *, L. Li n, T. Umezawa * & V. Chiang n

author keywords: Aspen; Transgenic; 4-Coumarate:CoA ligase; Coniferaldehyde 5-hydroxylase; Metabolic profiling
TL;DR: It is strongly suggested that the downregulation of 4CL and upregulation of CAld5H disrupt the metabolic flow through the cinnamate/monolignol pathway and thus alter the amount and structure of its final product, lignin. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2009 review

Towards a Systems Approach for Lignin Biosynthesis in Populus trichocarpa: Transcript Abundance and Specificity of the Monolignol Biosynthetic Genes

[Review of ]. PLANT AND CELL PHYSIOLOGY, 51(1), 144–163.

By: R. Shi n, Y. Sun n, Q. Li  n, S. Heber n , R. Sederoff n & V. Chiang n

author keywords: Lignin systems biology; Monolignol biosynthesis; Populus trichocarpa; Promoter motifs; Transcript abundance; Xylem-specific expression
MeSH headings : Amino Acid Motifs / genetics; Biosynthetic Pathways / genetics; Enzymes / biosynthesis; Enzymes / genetics; Gene Expression Regulation, Enzymologic / physiology; Gene Expression Regulation, Plant / physiology; Genome, Plant / genetics; Lignin / biosynthesis; Lignin / genetics; Phloem / enzymology; Phloem / genetics; Plant Shoots / enzymology; Plant Shoots / genetics; Populus / genetics; Populus / metabolism; Promoter Regions, Genetic / genetics; RNA, Messenger / analysis; RNA, Messenger / genetics; RNA, Messenger / metabolism; RNA, Plant / analysis; RNA, Plant / genetics; RNA, Plant / metabolism; Transcription, Genetic / physiology; Xylem / enzymology; Xylem / genetics
TL;DR: The identified 23 genes that most probably encode monolignol biosynthesis enzymes during wood formation are identified and evidence suggesting functional redundancy at the transcript level for phenylalanine ammonia-lyase and cinnamate 4-hydroxylase is found. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2008 journal article

A novel O-Methyltransferase-like gene with a drastic extopic expression in response to tension wood formation in Poupulus trichocarpa

Cellulose Chemistry and Technology, 41(9-10), 521–528.

By: T. Yeh, J. Wang, S. Rui, Y. Sun & V. Chiang

Source: NC State University Libraries
Added: August 6, 2018

2008 article

Differential expression of three eucalyptus secondary cell wall-related cellulose synthase genes in response to tension stress

Lu, S., Li, L., Yi, X., Joshi, C. P., & Chiang, V. L. (2008, February). JOURNAL OF EXPERIMENTAL BOTANY, Vol. 59, pp. 681–695.

By: S. Lu n, L. Li  n, X. Yi  n, C. Joshi* & V. Chiang n

author keywords: cellulose; cellulose synthase; cell wall; Eucalyptus grandis; tension stress; wood formation
MeSH headings : Adaptation, Physiological; Cell Wall / enzymology; Cloning, Molecular; DNA, Complementary; Eucalyptus / enzymology; Eucalyptus / genetics; Eucalyptus / physiology; Gene Expression Regulation, Plant; Glucosyltransferases / genetics; Glucosyltransferases / metabolism; Isoenzymes / genetics; Isoenzymes / metabolism; Promoter Regions, Genetic; Sequence Alignment; Sequence Homology, Nucleic Acid; Stress, Mechanical; Trees / enzymology; Trees / genetics; Trees / physiology; Wood / metabolism; Xylem / enzymology; Xylem / growth & development
TL;DR: The cloning and characterization of three CesA genes from an economically important tree species, Eucalyptus grandis, and several negative and positive regulatory regions controlling gene expression in xylem or phloem were identified and a region which is likely to contain mechanical stress-responsive elements was deduced. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Paxillus involutus Forms an Ectomycorrhizal Symbiosis and Enhances Survival of PtCOMT-modified Betula pendula in vitro

SILVAE GENETICA, 57(4-5), 235–242.

By: H. Tiimonen, T. Aronen, T. Laakso, P. Saranpaa, V. Chiang n, H. Haggman*, K. Niemi*

author keywords: Betula pendula; COMT; ecological impacts of gmtrees; ectomycorrhiza; lignin modification; Paxillus involutus
TL;DR: The results show that B. pendula plants genetically transformed with the lignin gene PtCOMT could form mycorrhizal symbiosis regardless of changes in either the root S/G ratio or development. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2008 journal article

Roles of 5-hydroxyconiferylaldehyde and caffeoyl CoA O-methyltransferases in monolignol biosynthesis in Carthamus tinctorius

Cellulose Chemistry and Technology, 41(9-10), 511–520.

By: T. Nakatsubo, L. Li, T. Hattori, S. Lu, N. Sakakibara, V. Chiang, M. Shimada, S. Suzuki, T. Umezawa

Source: NC State University Libraries
Added: August 6, 2018

2008 journal article

Stress-responsive microRNAs in Populus

PLANT JOURNAL, 55(1), 131–151.

By: S. Lu n, Y. Sun n & V. Chiang n

author keywords: microRNA; Populus trichocarpa; abiotic stress; cold stress; trees
MeSH headings : Base Sequence; Blotting, Northern; Cloning, Molecular; Cold Temperature; Gene Expression Profiling; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Genes, Plant; Hot Temperature; MicroRNAs / metabolism; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; Populus / genetics; Populus / physiology; Salinity; Stress, Mechanical; Water / physiology
TL;DR: The cloning of small RNAs from abiotic stressed tissues of Populus trichocarpa (Ptc) and the identification of 68 putative miRNA sequences that can be classified into 27 families based on sequence homology are reported, which suggests that the members of a family may have different functions. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2007 review

MicroRNAs in loblolly pine (Pinus taeda L.) and their association with fusiform rust gall development

[Review of ]. PLANT JOURNAL, 51(6), 1077–1098.

By: S. Lu n, Y. Sun n, H. Amerson n & V. Chiang n

author keywords: microRNA; Pinus taeda L.; Cronartium quercuum f. sp fusiforme; fusiform rust gall; non-protein coding transcript; ta-siRNA
MeSH headings : Base Sequence; Basidiomycota / physiology; Cloning, Molecular; Conserved Sequence; Genomics; MicroRNAs / chemistry; MicroRNAs / metabolism; MicroRNAs / physiology; Molecular Sequence Data; Pinus taeda / genetics; Pinus taeda / microbiology; Plant Diseases / genetics; Polymerase Chain Reaction; Sequence Analysis, RNA; Xylem / genetics
TL;DR: A new genetic basis for host-pathogen interactions in the development of fusiform rust gall is revealed and 82 plant disease-related transcripts that may also response to miRNA regulation in pine are predicted. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2007 conference paper

Microwave pretreatment of switchgrass to enhance enzymatic hydrolysis

Proceedings of the ASABE Annual International Meeting (Minneapolis, Minnesota), 077127.

By: D. Keshwani*, J. Cheng, L. Li*, J. Burns* & V. Chiang*

Source: NC State University Libraries
Added: August 6, 2018

2007 journal article

The seasonal activity and the effect of mechanical bending and wounding on the PtCOMT promoter in Betula pendula Roth

PLANT CELL REPORTS, 26(8), 1205–1214.

By: H. Tiimonen, H. Haggman *, C. Tsai *, V. Chiang n & T. Aronen

author keywords: Betula pendula; COMT; expression pattern; GUS; promoter
MeSH headings : Betula / genetics; Betula / metabolism; Gene Expression Regulation, Plant; Plant Roots / genetics; Plant Roots / metabolism; Plant Stems / anatomy & histology; Plant Stems / genetics; Plant Stems / metabolism; Plants, Genetically Modified; Promoter Regions, Genetic / genetics; Seasons; Stress, Mechanical
TL;DR: A strong GUS expression was induced by the PtCOMT promoters in response to mechanical stem bending but not to wounding, and may contribute to the biosynthesis of phenylpropanoid pathway-derived compounds other than lignin. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2006 review

A genomic and molecular view of wood formation

[Review of ]. CRITICAL REVIEWS IN PLANT SCIENCES, 25(3), 215–233.

By: L. Li n, S. Lu n & V. Chiang n

author keywords: secondary growth; wood formation; xylem differentiation; lignin; cellulose; hemicellulose; cambium meristem; cell wall
TL;DR: The first tree genome sequence (Populus genome) provides a valuable genomic resource for characterization of plant secondary growth and the function of many genes involved in wood biosyntheses and xylem differentiation has been characterized. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Distinct roles of cinnamate 4-hydroxylase genes in Populus

PLANT AND CELL PHYSIOLOGY, 47(7), 905–914.

By: S. Lu n, Y. Zhou  n, L. Li n & V. Chiang n

author keywords: cinnamate 4-hydroxylase (C4H); lignin; phenylpropanoid; Populus tremuloides; Populus trichocarpa
MeSH headings : DNA, Plant / genetics; Gene Expression Regulation, Enzymologic / genetics; Gene Expression Regulation, Plant / genetics; Genes, Plant / genetics; Genes, Plant / physiology; Isoenzymes / genetics; Isoenzymes / physiology; Lignin / biosynthesis; Molecular Sequence Data; Populus / enzymology; Populus / genetics; Reverse Transcriptase Polymerase Chain Reaction; Trans-Cinnamate 4-Monooxygenase / genetics; Trans-Cinnamate 4-Monooxygenase / metabolism
TL;DR: Sequential analysis of C4H promoters showed that the differential expression of C 4H genes was associated with cis-acting regulatory elements such as box L, box P and H box, suggesting that the divergent C4h isoforms played distinct roles in the production of secondary metabolites. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Genetic transformation of Populus trichocarpa genotype Nisqually-1: A functional genomic tool for woody plants

PLANT AND CELL PHYSIOLOGY, 47(11), 1582–1589.

By: J. Song n, S. Lu n, Z. Chen*, R. Lourenco n & V. Chiang n

author keywords: Agrobacterium tumefaciens; black cottonwood; functional genomics; genetic transformation; Nisqually-1; Populus trichocarpa
MeSH headings : Agrobacterium tumefaciens; Genome, Plant / genetics; Genomics / methods; Genotype; Glucuronidase / metabolism; Plants, Genetically Modified; Populus / genetics; Populus / microbiology; Populus / physiology; Regeneration; Transformation, Genetic; Wood / genetics; Wood / microbiology; Wood / physiology
TL;DR: The Agrobacterium-mediated genetic transformation of Nisqually-1, a Populus trichocarpa genotype whose genome was recently sequenced, is reported here to facilitate studies of gene functions in tree growth and development at a genome level. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2006 article

Monolignol biosynthesis and genetic engineering of lignin in trees, a review

Chiang, V. L. (2006, August). ENVIRONMENTAL CHEMISTRY LETTERS, Vol. 4, pp. 143–146.

By: V. Chiang n

TL;DR: This paper summarizes the previous and current research on genetic engineering of lignin biosynthesis for the purposes of improving wood pulping and bleaching efficiency and proves strong evidence for a principle phenolic flux leading to the formation of monolignols. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Plant growth, biomass partitioning and soil carbon formation in response to altered lignin biosynthesis in Populus tremuloides

NEW PHYTOLOGIST, 173(4), 732–742.

By: J. Hancock*, W. Loya*, C. Giardina *, L. Li n, V. Chiang n & K. Pregitzer*

author keywords: carbon cycling; leaf area; lignin; photosynthesis; plant production; Populus; tissue quality; transgenic aspen
MeSH headings : Biomass; Carbon / metabolism; Carbon Isotopes / metabolism; Lignin / biosynthesis; Nitrogen / metabolism; Photosynthesis; Plant Leaves / growth & development; Plant Leaves / metabolism; Plant Stems / metabolism; Plants, Genetically Modified; Populus / growth & development; Populus / metabolism; Soil; Soil Microbiology; Trees
TL;DR: The findings suggest that altered stem lignin biosynthesis in Populus may have little effect on the chemistry of fine roots or leaves, but can still have large effects on plant growth, biomass partitioning and soil C formation. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2006 journal article

Rapid analysis of transgenic trees using transmittance near-infrared spectroscopy (NIR)

HOLZFORSCHUNG, 60(1), 24–28.

By: T. Yamada n, T. Yeh n, H. Chang n, L. Li n, J. Kadla* & V. Chiang n

author keywords: cellulose; lignin; near-infrared spectroscopy (NIR); S/G ratio; transgenic tree; transmittance; xylose
Source: Web Of Science
Added: August 6, 2018

2006 journal article

The cellulose synthase gene superfamily and biochemical functions of xylem-specific cellulose synthase-like genes in Populus trichocarpa

PLANT PHYSIOLOGY, 142(3), 1233–1245.

By: S. Suzuki n, L. Li n, Y. Sun n & V. Chiang n

MeSH headings : Animals; Cell Line; Cloning, Molecular; Drosophila / cytology; Gene Expression Profiling; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Genome, Plant; Glucosyltransferases / genetics; Glucosyltransferases / metabolism; Multigene Family; Phylogeny; Populus / enzymology; Xylem / enzymology
TL;DR: Using Populus trichocarpa as a model angiosperm tree, a systematic analysis in various tissues of the absolute transcript copy numbers of cellulose synthase superfamily genes, the cellulose synthetase (CesA) and the hemicellulose-related cellulOSE synthase-like (Csl) genes is reported. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Clarification of cinnamoyl co-enzyme a reductase catalysis in monolignol biosynthesis of aspen

PLANT AND CELL PHYSIOLOGY, 46(7), 1073–1082.

By: L. Li n, X. Cheng n, S. Lu n, T. Nakatsubo*, T. Umezawa * & V. Chiang n

author keywords: cinnamoyl co-enzyme A reductase (CCR); lignin; monolignol biosynthesis; xylem
MeSH headings : Acyl Coenzyme A / chemistry; Acyl Coenzyme A / metabolism; Aldehyde Oxidoreductases / genetics; Aldehyde Oxidoreductases / isolation & purification; Aldehyde Oxidoreductases / metabolism; Catalysis; Chromatography, High Pressure Liquid; Cloning, Molecular; Esters / chemistry; Esters / metabolism; Gene Expression Profiling; Gene Expression Regulation, Plant; Genome, Plant; Hydrogen-Ion Concentration; Kinetics; Lignin / biosynthesis; Mass Spectrometry; Molecular Sequence Data; Populus / classification; Populus / enzymology; Populus / genetics; Populus / metabolism; Sequence Homology, Nucleic Acid; Substrate Specificity
TL;DR: The in vitro results revealed that the reactions mediated by these two neighboring enzymes require different pH environments, indicating that compartmentalization is probably needed for CCR and CCoAOMT to function properly in vivo. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Does lignin modification affect feeding preference or growth performance of insect herbivores in transgenic silver birch (Betula pendula Roth)?

PLANTA, 222(4), 699–708.

By: H. Tiimonen, T. Aronen, T. Laakso, P. Saranpaa, V. Chiang n, T. Ylioja *, H. Roininen, H. Haggman*

author keywords: Betula; insect herbivores; lignin modification; O-methyltransferase; syringyl/guaiacyl ratio
MeSH headings : Animals; Betula / genetics; Betula / metabolism; Coleoptera / growth & development; Coleoptera / physiology; Feeding Behavior; Genes, Plant; Insecta / growth & development; Insecta / physiology; Lepidoptera / growth & development; Lepidoptera / physiology; Lignin / metabolism; Plant Leaves / anatomy & histology; Plants, Genetically Modified / metabolism
TL;DR: The decreased syringyl/guaiacyl (S/G) ratio was found in stem and leaf lignin of 35S CaMV-Pt COMT transgenic silver birch lines when compared to non-transformed control or UbB1–PtCOMT lines. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2005 journal article

Facile means for quantifying microRNA expression by real-time PCR

BIOTECHNIQUES, 39(4), 519–525.

By: R. Shi n & V. Chiang n

MeSH headings : Arabidopsis / genetics; Base Sequence; Benzothiazoles; Blotting, Northern; DNA Primers / chemistry; DNA, Complementary / metabolism; Diamines; Fluorescent Dyes / pharmacology; Genes, Plant / genetics; MicroRNAs / analysis; Molecular Sequence Data; Organic Chemicals / pharmacology; Plant Leaves / metabolism; Plant Stems / metabolism; Polymerase Chain Reaction; Quinolines; RNA / metabolism; Reverse Transcriptase Polymerase Chain Reaction / methods; Sensitivity and Specificity; Temperature; Tissue Distribution; Transcription, Genetic
TL;DR: A simple and sensitive real-time PCR method for quantifying the expression of plant miRNAs and revealed miRNA tissue-specific expression patterns that cannot be resolved by Northern blot analysis and may therefore be widely useful for characterizing miRNA expression in plants as well as in animals. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
10. Reduced Inequalities (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2005 patent

Methods for simultaneous control of lignin content and composition, and cellulose content in plants

Washington, DC: U.S. Patent and Trademark Office.

By: V. Chiang & L. Li

Source: NC State University Libraries
Added: August 6, 2018

2005 journal article

Novel and mechanical stress-responsive microRNAs in Populus trichocarpa that are absent from Arabidopsis

Plant Cell, 17(8), 2186–2203.

By: S. Lu n, Y. Sun n, R. Shi n, C. Clark n, L. Li n & V. Chiang n

MeSH headings : Arabidopsis / genetics; Base Sequence; Conserved Sequence; Gene Expression Regulation, Plant; MicroRNAs / chemistry; MicroRNAs / genetics; Molecular Sequence Data; Plant Stems / genetics; Populus / genetics; RNA, Plant / genetics; Species Specificity; Transcription, Genetic
TL;DR: It is shown that plant miRNAs can be induced by mechanical stress and may function in one of the most critical defense systems for structural and mechanical fitness. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018

2005 journal article

Rapid screening of wood chemical component variations using transmittance near-infrared spectroscopy

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 53(9), 3328–3332.

By: T. Yeh  n, T. Yamada n, E. Capanema n, H. Chang n, V. Chiang n & J. Kadla n

author keywords: Loblolly pine (Pinus taeda); aspen (Populus trichocarpa); transmittance near-infrared spectroscopy (NIR); increment cores; wood wafer; alpha-cellulose content; lignin content; screening
MeSH headings : Cellulose / analysis; Lignin / analysis; Pinus taeda / chemistry; Populus / chemistry; Spectroscopy, Near-Infrared / methods; Wood
TL;DR: A rapid transmittance near-infrared (NIR) spectroscopy method was developed to predict the variation in chemical composition of solid wood and correlations were obtained between laboratory wet chemistry values and the NIR-predicted values. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2004 patent

Genetic engineering of syringyl-enriched lignin in plants

Washington, DC: U.S. Patent and Trademark Office.

By: V. Chiang & L. Li

Source: NC State University Libraries
Added: August 6, 2018

2004 journal article

RNA silencing in plants by the expression of siRNA duplexes

NUCLEIC ACIDS RESEARCH, 32(21).

By: S. Lu n, R. Shi, C. Tsao, X. Yi, L. Li & V. Chiang

MeSH headings : Gene Expression Regulation, Plant; Genetic Vectors; Glucuronidase / analysis; Glucuronidase / genetics; Humans; Molecular Sequence Data; Promoter Regions, Genetic; RNA / genetics; RNA Interference; RNA, Small Cytoplasmic / genetics; RNA, Small Interfering / biosynthesis; RNA, Small Interfering / chemistry; RNA, Small Interfering / genetics; Signal Recognition Particle / genetics; Tobacco / genetics; Tobacco / metabolism
TL;DR: It is reported that RNA silencing was achieved in plants through stable expression of short hairpin RNA, which was driven by Pol III RNA gene promoters, and the effectiveness of the human H1 promoter in a plant background suggested a conserved mechanism underlying Pol III complex functionality. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Combinatorial modification of multiple lignin traits in trees through multigene cotransformation

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 100(8), 4939–4944.

By: L. Li*, Y. Zhou *, X. Cheng *, J. Sun*, J. Marita*, J. Ralph *, V. Chiang*

MeSH headings : Base Sequence; Coenzyme A Ligases / genetics; Cytochrome P-450 Enzyme System / genetics; DNA, Plant / genetics; Genes, Plant; Immunohistochemistry; Lignin / chemistry; Lignin / metabolism; Magnetic Resonance Spectroscopy; Mixed Function Oxygenases / genetics; Models, Biological; Plant Proteins; Plants, Genetically Modified; Populus / genetics; Populus / metabolism; Rhizobium / genetics; Transformation, Genetic
TL;DR: The results suggest that this multigene cotransfer system should be broadly useful for plant genetic engineering and functional genomics. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2002 journal article

Differential substrate inhibition couples kinetically distinct 4-coumarate: Coenzyme A ligases with spatially distinct metabolic roles in quaking aspen

Plant Physiology, 128(2002), 428–438.

By: S. Harding *, J. Leshkevich*, V. Chiang* & C. Tsai *

MeSH headings : Acyl Coenzyme A / metabolism; Caffeic Acids / metabolism; Cloning, Molecular; Coenzyme A Ligases / genetics; Coenzyme A Ligases / metabolism; Coumaric Acids / metabolism; Flavonoids / biosynthesis; Flavonoids / chemistry; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; In Situ Hybridization; Isoenzymes / genetics; Isoenzymes / metabolism; Kinetics; Lignin / biosynthesis; Lignin / chemistry; Pinus / enzymology; Pinus / genetics; Plant Stems / cytology; Plant Stems / enzymology; Plant Stems / genetics; Promoter Regions, Genetic; Recombinant Proteins / metabolism; Salicaceae / enzymology; Salicaceae / genetics
TL;DR: Single and mixed-substrate assays were conducted to determine whether the 4CLs from aspen exhibit clear catalytic identities under certain metabolic circumstances, and to advance a mechanism by which 4CL1 can selectively utilize caffeate for the support of monolignol biosynthesis in maturing xylem and phloem fibers. (via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018

2002 journal article

From rags to riches

NATURE BIOTECHNOLOGY, 20(6), 557–558.

By: V. Chiang*

MeSH headings : Ecosystem; Environmental Pollution; Industrial Waste / prevention & control; Lignin / genetics; Lignin / metabolism; Models, Chemical; Paper; Plants, Genetically Modified; Trees / genetics; Trees / metabolism; Wood
Source: Web Of Science
Added: August 6, 2018

2002 patent

Methods of modifying lignin in plants by transformation with a 4-coumarate coenzyme a ligase nucleic acid

Washington, DC: U.S. Patent and Trademark Office.

By: V. Chiang, C. Tsai & W. Hu

Source: NC State University Libraries
Added: August 6, 2018

1999 patent

Genetic engineering of wood color in plants

Washington, DC: U.S. Patent and Trademark Office.

By: V. Chiang, C. Tsai & G. Podila

Source: NC State University Libraries
Added: August 6, 2018

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