@article{dutta_altermann_crespo_olson_siletzky_kathariou_2017, title={Identification of a Campylobacter coli methyltransferase targeting adenines at GATC sites}, volume={364}, DOI={10.1093/femsle/fnw268}, abstractNote={Abstract Campylobacter coli can infect humans and colonize multiple other animals, but its host‐associated genes or adaptations are poorly understood. Adenine methylation at GATC sites, resulting in MboI resistance of genomic DNA, was earlier frequently detected among C. coli from swine but not among turkey‐derived isolates. The underlying genetic basis has remained unknown. Comparative genome sequence analyses of C. coli 6461, a swine‐derived strain with MboI‐resistant DNA, revealed two chromosomal ORFs, 0059 and 0060, encoding a putative DNA methyltransferase and a conserved hypothetical protein, respectively, which were lacking from the genome of the turkey‐derived C. coli strain 11601, which had MboI‐susceptible DNA. To determine whether ORF0059 mediated MboI resistance and hence encoded a putative N6‐adenine DNA methyltransferase, the gene was cloned immediately upstream of a chloramphenicol resistance cassette (cat) and a PCR fragment harboring ORF0059‐cat was transformed into C. coli 11601. The transformants had MboI‐resistant DNA, suggesting a direct role of this gene in methylation of adenines at GATC sites. In silico analyses suggested that the ORF0059‐ORF0060 cassette was more frequent among C. coli from swine than certain other sources (e.g. cattle, humans). Potential impacts of ORF0059‐mediated methylation on C. coli host preference and other adaptations remain to be elucidated.}, number={7}, journal={FEMS Microbiology Letters}, author={Dutta, V. and Altermann, E. and Crespo, M. D. and Olson, J. W. and Siletzky, R. M. and Kathariou, S.}, year={2017} }
 @article{dutta_elhanafi_osborne_martinez_kathariou_2014, title={Genetic Characterization of Plasmid-Associated Triphenylmethane Reductase in Listeria monocytogenes}, volume={80}, ISSN={["1098-5336"]}, DOI={10.1128/aem.01398-14}, abstractNote={ABSTRACT}, number={17}, journal={APPLIED AND ENVIRONMENTAL MICROBIOLOGY}, author={Dutta, Vikrant and Elhanafi, Driss and Osborne, Jason and Martinez, Mira Rakic and Kathariou, Sophia}, year={2014}, month={Sep}, pages={5379–5385} }
 @article{dutta_elhanafi_kathariou_2013, title={Conservation and Distribution of the Benzalkonium Chloride Resistance Cassette bcrABC in Listeria monocytogenes}, volume={79}, ISSN={["1098-5336"]}, DOI={10.1128/aem.01751-13}, abstractNote={ABSTRACT}, number={19}, journal={APPLIED AND ENVIRONMENTAL MICROBIOLOGY}, author={Dutta, Vikrant and Elhanafi, Driss and Kathariou, Sophia}, year={2013}, month={Oct}, pages={6067–6074} }
 @article{ratani_siletzky_dutta_yildirim_osborne_lin_hitchins_ward_kathariou_2012, title={Heavy Metal and Disinfectant Resistance of Listeria monocytogenes from Foods and Food Processing Plants}, volume={78}, ISSN={["0099-2240"]}, DOI={10.1128/aem.01553-12}, abstractNote={ABSTRACT}, number={19}, journal={APPLIED AND ENVIRONMENTAL MICROBIOLOGY}, author={Ratani, Shakir S. and Siletzky, Robin M. and Dutta, Vikrant and Yildirim, Suleyman and Osborne, Jason A. and Lin, Wen and Hitchins, Anthony D. and Ward, Todd J. and Kathariou, Sophia}, year={2012}, month={Oct}, pages={6938–6945} }
 @article{huff_dutta_huff_rath_2011, title={Effects of dietary yeast extract on turkey stress response and heterophil oxidative burst activity}, volume={52}, ISSN={["1466-1799"]}, DOI={10.1080/00071668.2011.600753}, abstractNote={1. Effective nutritional approaches to counteract the negative effects of stress may provide food animal producers with useful alternatives to antibiotics. In this study, turkeys were fed on a standard diet, or the same diet supplemented with yeast extract (YE), to determine if YE would improve disease resistance in a stress model. 2. At 16 weeks of age, half of the birds were exposed to a bacterial challenge using a coarse spray of the pen environment. A subset of control and challenged birds was also treated with dexamethasone (Dex) prior to challenge (Dex/challenge). At 18 weeks, another subset was subjected to a 12 h transport stress protocol (Challenge/transport). All birds were bled and necropsied the morning after transport. The numbers and proportions of blood cells and the heterophil oxidative burst activity (OBA) were determined. Serum corticosterone (Cort) levels of male birds were measured using a commercial ELISA kit. Body weight and gain were increased by YE during week 1. 3. YE decreased mortality and bacterial isolation following Dex/challenge only in females. Cort levels in male turkeys were decreased by YE and Dex treatment. OBA was higher in males and in birds given YE and was reduced by challenge and transport. 4. These results suggest there may be gender differences in the turkey stress response and that dietary YE has potential for modulating the impact of stress on innate immunity of turkeys.}, number={4}, journal={BRITISH POULTRY SCIENCE}, author={Huff, G. R. and Dutta, V. and Huff, W. E. and Rath, N. C.}, year={2011}, pages={446–455} }
 @article{rakic-martinez_drevets_dutta_katic_kathariou_2011, title={Listeria monocytogenes Strains Selected on Ciprofloxacin or the Disinfectant Benzalkonium Chloride Exhibit Reduced Susceptibility to Ciprofloxacin, Gentamicin, Benzalkonium Chloride, and Other Toxic Compounds}, volume={77}, ISSN={["1098-5336"]}, DOI={10.1128/aem.05941-11}, abstractNote={ABSTRACT}, number={24}, journal={APPLIED AND ENVIRONMENTAL MICROBIOLOGY}, author={Rakic-Martinez, Mira and Drevets, Douglas A. and Dutta, Vikrant and Katic, Vera and Kathariou, Sophia}, year={2011}, month={Dec}, pages={8714–8721} }
 @article{elhanafi_dutta_kathariou_2010, title={Genetic Characterization of Plasmid-Associated Benzalkonium Chloride Resistance Determinants in a Listeria monocytogenes Strain from the 1998-1999 Outbreak}, volume={76}, ISSN={["1098-5336"]}, DOI={10.1128/aem.02056-10}, abstractNote={ABSTRACT}, number={24}, journal={APPLIED AND ENVIRONMENTAL MICROBIOLOGY}, author={Elhanafi, Driss and Dutta, Vikrant and Kathariou, Sophia}, year={2010}, month={Dec}, pages={8231–8238} }
 @article{huff_huff_dutta_johnson_nannapaneni_2008, title={Pathogenicity of Listeria monocytogenes Scott A After Oral and Oculonasal Challenges of Day-Old Turkey Poults}, volume={52}, ISSN={["1938-4351"]}, DOI={10.1637/8244-013008-Reg.1}, abstractNote={Abstract Listeria monocytogenes is a ubiquitous, environmental pathogen that has contaminated poultry ready-to-eat products resulting in large-scale recalls. Research is needed to determine the source of product and processing plant contamination with L. monocytogenes. The purpose of this study was to compare the oral and oculonasal routes of infection on the pathogenicity of L. monocytogenes in turkey poults under different housing conditions. One-day-old turkey poults were challenged by either route with the Scott A strain of L. monocytogenes and placed either in paper-lined battery-brooder cages for 1 wk or in floor pens on fresh pine-shaving litter. On day 7, birds challenged in battery cages were transferred to floor pens. Challenge by the oculonasal route resulted in higher mortality (P  =  0.05) and lower body weights (P < 0.0001) compared with both nonchallenged controls and those challenged by the oral route. Birds contained in battery cages for 1 wk had higher mortality (P  =  0.002) and higher body weights (P < 0.0001) compared with floor-pen–reared birds. Using direct plating, the challenge strain was isolated from the gall bladder, brain, and knee joint of only one dead poult challenged by the oculonasal route. These results suggest that day-old turkey poults may be more susceptible to an oculonasal challenge with L. monocytogenes than to an oral challenge and that containment in battery cages for the first week increased contact exposure to the challenge. Patogenicidad de Listeria monocytogenes Scott A luego del desafío oral y oculonasal de pavitos de un día de edad. La Listeria monocytogenes es un patógeno ambiental ubicuo que ha contaminado productos avícolas listos para consumir resultando en retiros masivos del mercado. Se requiere investigación para determinar el origen de la contaminación con L. monocytogenes de productos y de la planta de procesamiento. El propósito de este estudio fue comparar el efecto de las rutas de infección oral y óculonasal en la patogenicidad de L. monocytogenes en pavos jóvenes bajo diferentes condiciones de crianza. Se desafiaron pavitos de un día de edad con L. monocytogenes por alguna de las dos rutas y se colocaron en jaulas de crianza con papel en el piso por una semana o se colocaron en corrales con cama de viruta de pino. Al día siete, las aves desafiadas en las jaulas se transfirieron a los corrales en piso. El desafío por la vía óculonasal resultó en mayor mortalidad (P  =  0.05) y en pesos corporales mas bajos (P < 0.0001), en comparación con los controles no desafiados y las aves desafiadas por la vía oral. Las aves mantenidas en las jaulas por una semana presentaron una mayor mortalidad (P  =  0.002) y mayor peso corporal (P < 0.0001) que las aves criadas en el piso. Utilizando siembra directa, se aisló la cepa de desafío de la vesícula biliar, cerebro y articulación de la rodilla de sólo una de las aves muertas desafiada por la vía óculonasal. Estos resultados sugieren que los pavitos de un día de edad pueden ser más susceptibles a un desafío óculonasal con L. monocytogenes que a un desafío oral y que el confinamiento en jaulas durante la primera semana incrementó la exposición por contacto al desafío. Abbreviations: Baso = basophils; CFU = colony forming units; EDTA = ethylenediaminetetraacetic acid; Eos = eosinophils; FAC = ferric ammonium citrate; Het = heterophils; Lym = lymphocytes; Mono = monocytes; plt = platelets; TPB = tryptose phosphate broth; UVM = University of Vermont medium; WBC = white blood cells}, number={3}, journal={AVIAN DISEASES}, author={Huff, G. R. and Huff, W. E. and Dutta, V. and Johnson, M. G. and Nannapaneni, R.}, year={2008}, month={Sep}, pages={444–450} }