William Anthony Klobasa

Works (6)

Updated: June 18th, 2024 05:04

2024 article

CRISPR/Cas9-mediated genome editing of <i>Frankliniella occidentalis</i>, the western flower thrips, via embryonic microinjection

Han, J., Klobasa, W., Oliveira, L., Rotenberg, D., Whitfield, A. E., & Lorenzen, M. D. (2024, April 27). INSECT MOLECULAR BIOLOGY, Vol. 4.

By: J. Han  n, W. Klobasa n, L. Oliveira  n, D. Rotenberg n , A. Whitfield n & M. Lorenzen n 

author keywords: CRISPR; eye; gene knockout; insect vector; Thysanoptera
Sources: Web Of Science, NC State University Libraries
Added: May 7, 2024

2024 journal article

Evaluation of <i>Peregrinus maidis transformer-2</i> as a target for CRISPR-based control

PLOS ONE, 19(4).

By: Y. Wang n, D. Rivera n, W. Klobasa n & M. Lorenzen n 

UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: June 17, 2024

2023 journal article

Structural and functional insights into the ATP-binding cassette transporter family in the corn planthopper, Peregrinus maidis

Insect Molecular Biology, 32(4), 412–423.

By: Y. Wang n, W. Klobasa n, F. Chu n, O. Huot n, A. Whitfield n & M. Lorenzen n 

author keywords: ABC transporters; Hemiptera; RNA interference (RNAi); transcriptome
TL;DR: Transcriptomic data and RNAi results presented here will no doubt assist with the development of new control methods for this pest and each phenotype is similar to that found when targeting the orthologous gene in other species and it demonstrates their potential in RNAi‐based P. maidis control. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: May 1, 2023

2021 journal article

Microinjection of Corn Planthopper, <em>Peregrinus maidis</em>, Embryos for CRISPR/Cas9 Genome Editing

Journal of Visualized Experiments, 3(169).

By: W. Klobasa n, F. Chu n, O. Huot n, N. Grubbs n, D. Rotenberg n , A. Whitfield n, M. Lorenzen  n

MeSH headings : Animals; CRISPR-Cas Systems / genetics; Endonucleases / genetics; Female; Gene Editing / methods; Zea mays / chemistry
TL;DR: Detailed protocols for collecting and microinjecting precellular P. Maidis embryos with CRISPR components (Cas9 nuclease that has been complexed with guide RNAs), and results of Cas9-based gene knockout of a P. maidis eye-color gene, white, are presented. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: May 17, 2021

2017 journal article

Development and use of a piggyBac -based jumpstarter system in Drosophila suzukii

Archives of Insect Biochemistry and Physiology, 97(3), e21439.

By: F. Chu n, W. Klobasa n, N. Grubbs n & M. Lorenzen  n

author keywords: germline transformation; Minos; mutagenesis; piggyBac; transgenesis
MeSH headings : Animals; Animals, Genetically Modified; DNA Transposable Elements; Drosophila / genetics; Genetic Engineering / methods; Mutagenesis; Transposases
TL;DR: It is demonstrated that there is a dramatic increase in transformation rates when germline transformation is performed in a transposase-expressing background, and it is shown that these jumpstarter strains could be used in conjunction with a piggyBac-based donor strain for genome-wide mutagenesis of D. suzukii. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

2017 journal article

Germline transformation of the western corn rootworm, Diabrotica virgifera virgifera

Insect Molecular Biology, 26(4), 440–452.

By: F. Chu n, W. Klobasa n, P. Wu n, S. Pinzi n, N. Grubbs n, S. Gorski  n, Y. Cardoza n, M. Lorenzen n 

author keywords: Minos; piggyBac; small-scale rearing; embryonic microinjection
MeSH headings : Animals; Coleoptera / genetics; Female; Gene Transfer Techniques; Luminescent Proteins; Male; Transposases
TL;DR: Establishing transgenic technologies for this beetle is the first step towards bringing a wide range of transformation‐based tools to bear on understanding WCR biology. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, Crossref
Added: August 6, 2018

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