Works (5)

Updated: July 5th, 2023 15:38

2017 journal article

Crystallization of a fungal lytic polysaccharide monooxygenase expressed from glycoengineered Pichia pastoris for X-ray and neutron diffraction

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 73(2), 70–78.

author keywords: lytic polysaccharide monooxygenases; Pichia pastoris; glycoengineering; neutron proteincrystallography
MeSH headings : Amino Acid Sequence; Catalytic Domain; Cloning, Molecular; Crystallography; Fungal Polysaccharides / chemistry; Fungal Polysaccharides / metabolism; Fungal Proteins / chemistry; Fungal Proteins / genetics; Fungal Proteins / metabolism; Gene Expression; Genetic Engineering; Mixed Function Oxygenases / chemistry; Mixed Function Oxygenases / genetics; Mixed Function Oxygenases / metabolism; Models, Molecular; Neurospora crassa / chemistry; Neurospora crassa / enzymology; Neutron Diffraction; Pichia / enzymology; Pichia / genetics; Plasmids / chemistry; Plasmids / metabolism; Protein Binding; Protein Conformation, beta-Strand; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Substrate Specificity; X-Ray Diffraction
TL;DR: Diffraction resolution and crystal morphology were improved by expressing NcPMO-2 from a glycoengineered strain of P. pastoris and by the use of crystal seeding methods, respectively, which resulted in high-resolution X-ray diffraction data collection at 100 K and the production of a large Nc PMO- 2 crystal suitable for room-temperature neutron diffractionData collection to 2.12 Å resolution. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2017 journal article

Oxygen Activation at the Active Site of a Fungal Lytic Polysaccharide Monooxygenase

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 56(3), 767–770.

author keywords: copper; oxidoreductases; oxygen activation; polysaccharide monooxygenases; protein structures
MeSH headings : Catalytic Domain; Mixed Function Oxygenases / chemistry; Mixed Function Oxygenases / metabolism; Models, Molecular; Neurospora crassa / enzymology; Oxygen / chemistry; Oxygen / metabolism; Polysaccharides / chemistry; Polysaccharides / metabolism
TL;DR: High-resolution X-ray crystal structures of an enzyme from Neurospora crassa in the resting state and of a copper(II) dioxo intermediate complex formed in the absence of substrate are determined, providing a new structural description of oxygen activation by substrate free lytic polysaccharide monooxygenases. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2017 journal article

Structural studies of Neurospora crassa LPMO9D and redox partner CDHIIA using neutron crystallography and small-angle scattering

CARBOHYDRATE RESEARCH, 448, 200–204.

author keywords: Polysaccharide monooxygenases; Cellobiose dehydrogenases; Neutron diffraction; Neutron scattering; X-ray induced photoreduction
MeSH headings : Carbohydrate Dehydrogenases / chemistry; Carbohydrate Dehydrogenases / metabolism; Catalytic Domain; Crystallography, X-Ray; Mixed Function Oxygenases / chemistry; Mixed Function Oxygenases / metabolism; Models, Molecular; Neurospora crassa / enzymology; Oxidation-Reduction; Scattering, Small Angle
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2016 journal article

Neutron protein crystallography: A complementary tool for locating hydrogens in proteins

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 602, 48–60.

author keywords: Neutron; Crystallography; Hydrogen; Enzyme; Protonation; Water
TL;DR: Methods to produce isotopically-substituted proteins and to grow large crystals are provided in the context of neutron structures reported in the literature along with technique-specific strategies including joint X-ray/neutron structure refinement. (via Semantic Scholar)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2013 journal article

On the structure of water and chloride ion interactions with a peptide backbone in solution

Physical Chemistry Chemical Physics, 15(48), 21023–21033.

By: S. Busch, L. Pardo, W. O'Dell, C. Bruce, C. Lorenz & S. McLain

Source: NC State University Libraries
Added: August 6, 2018

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