Works (5)
Updated: July 5th, 2023 15:38
2017 journal article
Crystallization of a fungal lytic polysaccharide monooxygenase expressed from glycoengineered Pichia pastoris for X-ray and neutron diffraction
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 73(2), 70–78.
author keywords: lytic polysaccharide monooxygenases; Pichia pastoris; glycoengineering; neutron proteincrystallography
MeSH headings : Amino Acid Sequence; Catalytic Domain; Cloning, Molecular; Crystallography; Fungal Polysaccharides / chemistry; Fungal Polysaccharides / metabolism; Fungal Proteins / chemistry; Fungal Proteins / genetics; Fungal Proteins / metabolism; Gene Expression; Genetic Engineering; Mixed Function Oxygenases / chemistry; Mixed Function Oxygenases / genetics; Mixed Function Oxygenases / metabolism; Models, Molecular; Neurospora crassa / chemistry; Neurospora crassa / enzymology; Neutron Diffraction; Pichia / enzymology; Pichia / genetics; Plasmids / chemistry; Plasmids / metabolism; Protein Binding; Protein Conformation, beta-Strand; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Substrate Specificity; X-Ray Diffraction
TL;DR:
Diffraction resolution and crystal morphology were improved by expressing NcPMO-2 from a glycoengineered strain of P. pastoris and by the use of crystal seeding methods, respectively, which resulted in high-resolution X-ray diffraction data collection at 100 K and the production of a large Nc PMO- 2 crystal suitable for room-temperature neutron diffractionData collection to 2.12 Å resolution.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2017 journal article
Oxygen Activation at the Active Site of a Fungal Lytic Polysaccharide Monooxygenase
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 56(3), 767–770.
author keywords: copper; oxidoreductases; oxygen activation; polysaccharide monooxygenases; protein structures
MeSH headings : Catalytic Domain; Mixed Function Oxygenases / chemistry; Mixed Function Oxygenases / metabolism; Models, Molecular; Neurospora crassa / enzymology; Oxygen / chemistry; Oxygen / metabolism; Polysaccharides / chemistry; Polysaccharides / metabolism
TL;DR:
High-resolution X-ray crystal structures of an enzyme from Neurospora crassa in the resting state and of a copper(II) dioxo intermediate complex formed in the absence of substrate are determined, providing a new structural description of oxygen activation by substrate free lytic polysaccharide monooxygenases.
(via Semantic Scholar)
europepmc.org (repository)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2017 journal article
Structural studies of Neurospora crassa LPMO9D and redox partner CDHIIA using neutron crystallography and small-angle scattering
CARBOHYDRATE RESEARCH, 448, 200–204.
author keywords: Polysaccharide monooxygenases; Cellobiose dehydrogenases; Neutron diffraction; Neutron scattering; X-ray induced photoreduction
MeSH headings : Carbohydrate Dehydrogenases / chemistry; Carbohydrate Dehydrogenases / metabolism; Catalytic Domain; Crystallography, X-Ray; Mixed Function Oxygenases / chemistry; Mixed Function Oxygenases / metabolism; Models, Molecular; Neurospora crassa / enzymology; Oxidation-Reduction; Scattering, Small Angle
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
7. Affordable and Clean Energy
(Web of Science)
12. Responsible Consumption and Production
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2016 journal article
Neutron protein crystallography: A complementary tool for locating hydrogens in proteins
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 602, 48–60.
author keywords: Neutron; Crystallography; Hydrogen; Enzyme; Protonation; Water
TL;DR:
Methods to produce isotopically-substituted proteins and to grow large crystals are provided in the context of neutron structures reported in the literature along with technique-specific strategies including joint X-ray/neutron structure refinement.
(via Semantic Scholar)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2013 journal article
On the structure of water and chloride ion interactions with a peptide backbone in solution
Physical Chemistry Chemical Physics, 15(48), 21023–21033.
Source: NC State University Libraries
Added: August 6, 2018
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