Works (57)

Updated: September 18th, 2023 08:06

2023 journal article

A Ferric-Superoxide Intermediate Initiates P450-Catalyzed Cyclic Dipeptide Dimerization

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 8.

By: H. Gering n, X. Li n, H. Tang n, P. Swartz n, W. Chang n & T. Makris n

Sources: Web Of Science, ORCID
Added: September 11, 2023

2023 article

Mechanistic Studies of Aziridine Formation Catalyzed by Mononuclear Non-Heme Iron Enzymes

Cha, L., Paris, J. C., Zanella, B., Spletzer, M., Yao, A., Guo, Y., & Chang, W.-chen. (2023, March 13). JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol. 3.

By: L. Cha n, J. Paris*, B. Zanella n, M. Spletzer*, A. Yao n, Y. Guo*, W. Chang n

MeSH headings : Iron / chemistry; Aziridines; Hydroxylation; Catalysis
Sources: Web Of Science, ORCID
Added: April 4, 2023

2023 article

Repurposing Iron- and 2-Oxoglutarate-Dependent Oxygenases to Catalyze Olefin Hydration

Wang, B., Lu, Y., Cha, L., Chen, T.-Y., Palacios, P. M., Li, L., … Chen, C. (2023, September 6). ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol. 9.

By: B. Wang*, Y. Lu*, L. Cha n, T. Chen n, P. Palacios*, L. Li*, Y. Guo*, W. Chang n, C. Chen*

author keywords: Biocatalysis; Hydrogen Atom Transfer; Mukaiyama Hydration; Nonheme Fe/2OG-Dependent Oxygenase
Sources: Web Of Science, ORCID
Added: September 11, 2023

2022 journal article

Deciphering the Reaction Pathway of Mononuclear Iron Enzyme-Catalyzed N C Triple Bond Formation in Isocyanide Lipopeptide and Polyketide Biosynthesis

ACS CATALYSIS, 12(4), 2270–2279.

By: T. Chen n, Z. Zheng*, X. Zhang*, J. Chen*, L. Cha n, Y. Tang*, Y. Guo*, J. Zhou* ...

author keywords: isocyanide; oxygenase; natural product; reaction mechanism; desaturation; C-N bond formation
Sources: Web Of Science, ORCID
Added: March 28, 2022

2022 journal article

Elucidation of divergent desaturation pathways in the formation of vinyl isonitrile and isocyanoacrylate

NATURE COMMUNICATIONS, 13(1).

By: W. Kim*, T. Chen n, L. Cha n, G. Zhou*, K. Xing*, N. Canty n, Y. Zhang*, W. Chang n

MeSH headings : Iron / metabolism; Ketoglutaric Acids / metabolism; Tyrosine
Sources: Web Of Science, ORCID
Added: September 26, 2022

2022 journal article

Mechanism of Methyldehydrofosmidomycin Maturation: Use Olefination to Enable Chain Elongation

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 144(18), 8257–8266.

By: X. Li n, S. Xue*, Y. Guo* & W. Chang n

MeSH headings : Alkenes / chemistry; Catalysis; Hydroxylation; Iron / chemistry; Ketoglutaric Acids / metabolism
Sources: Web Of Science, ORCID
Added: June 6, 2022

2022 journal article

Mechanistic analysis of carbon-carbon bond formation by deoxypodophyllotoxin synthase

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 119(1).

By: H. Tang n, M. Wu*, H. Lin*, M. Han*, Y. Tu*, Z. Yang*, T. Chien*, N. Chan*, W. Chang n

author keywords: C-C coupling; cyclization; oxygenase; natural product; reaction mechanism
MeSH headings : Berberidaceae / enzymology; Drugs, Chinese Herbal / chemistry; Ligases / chemistry; Oxidation-Reduction; Plant Proteins / chemistry; Podophyllotoxin / analogs & derivatives; Podophyllotoxin / chemistry
Sources: Web Of Science, ORCID
Added: February 21, 2022

2022 journal article

Self-sacrificial tyrosine cleavage by an Fe:Mn oxygenase for the biosynthesis of para-aminobenzoate in Chlamydia trachomatis

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 119(39).

By: O. Manley n, H. Phan n, A. Stewart n, D. Mosley n, S. Xue*, L. Cha n, H. Bai n, V. Lightfoot* ...

Contriutors: T. Williams n

author keywords: folate biosynthesis; radical transfer; spectroscopy; heterobinuclear cluster
MeSH headings : Bacterial Proteins / metabolism; Chlamydia trachomatis / enzymology; Folic Acid; Iron / metabolism; Manganese / metabolism; Oxygen / metabolism; Oxygenases / metabolism; Tyrosine / metabolism; para-Aminobenzoates / metabolism
Sources: Web Of Science, ORCID
Added: September 21, 2022

2021 article

A postreplicative C5-cytosine hypermodification triggered by bacteriophage methyltransferase and hydroxylase

Chen, T.-Y., & Chang, W.-chen. (2021, July 13). PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol. 118.

By: T. Chen n & W. Chang n

MeSH headings : Bacteriophages / enzymology; Cytosine / metabolism; DNA Replication; Glycosylation; Glycosyltransferases / metabolism; Methyltransferases / metabolism; Mixed Function Oxygenases / metabolism; Substrate Specificity; Viral Proteins / metabolism
Sources: Web Of Science, ORCID
Added: August 23, 2021

2021 article

BesC Initiates C-C Cleavage through a Substrate-Triggered and Reactive Diferric-Peroxo Intermediate

Manley, O. M., Tang, H., Xue, S., Guo, Y., Chang, W.-chen, & Makris, T. M. (2021, December 13). JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol. 12.

By: O. Manley n, H. Tang n, S. Xue*, Y. Guo*, W. Chang n & T. Makris n

MeSH headings : Carbon / chemistry; Carbon / metabolism; Ferric Compounds / chemistry; Oxidoreductases / metabolism; Oxygen / chemistry; Spectroscopy, Mossbauer; Streptomyces / enzymology; Substrate Specificity
Sources: Web Of Science, ORCID
Added: January 3, 2022

2021 journal article

Current Understanding toward Isonitrile Group Biosynthesis and Mechanism

CHINESE JOURNAL OF CHEMISTRY, 39(2), 463–472.

author keywords: Biosynthesis; Enzymes; Natural products; Reaction mechanisms; Biotransformations
Sources: Web Of Science, ORCID
Added: March 1, 2021

2021 journal article

Deciphering Pyrrolidine and Olefin Formation Mechanism in Kainic Acid Biosynthesis

ACS CATALYSIS, 11(1), 278–282.

By: T. Chen n, S. Xue*, W. Tsai*, T. Chien, Y. Guo* & W. Chang n

author keywords: metalloenzyme; C-H activation; cyclization; desaturation; enzyme mechanism
Sources: Web Of Science, ORCID
Added: January 11, 2021

2021 journal article

Harnessing the Substrate Promiscuity of Dioxygenase AsqJ and Developing Efficient Chemoenzymatic Synthesis for Quinolones

ACS CATALYSIS, 11(12), 7186–7192.

By: H. Tang n, Y. Tang*, I. Kurnikov*, H. Liao*, N. Chan*, M. Kurnikova*, Y. Guo*, W. Chang n

author keywords: Fe/2OG enzyme; AsqJ; chemoenzymatic synthesis; rearrangement; quinolone
Sources: Web Of Science, ORCID
Added: July 19, 2021

2021 article

Identification of Cyclopropane Formation in the Biosyntheses of Hormaomycins and Belactosins: Sequential Nitration and Cyclopropanation by Metalloenzymes

Li, X., Shimaya, R., Dairi, T., Chang, W.-chen, & Ogasawara, Y. (2021, December 27). ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol. 12.

By: X. Li n, R. Shimaya*, T. Dairi*, W. Chang n & Y. Ogasawara

author keywords: Biosynthesis; Cyclopropane; Metalloenzymes; Natural products; Peptides
MeSH headings : Catalysis; Cyclopropanes / chemistry; Cyclopropanes / metabolism; Depsipeptides / chemistry; Depsipeptides / metabolism; Intercellular Signaling Peptides and Proteins / chemistry; Intercellular Signaling Peptides and Proteins / metabolism; Metalloproteins / chemistry; Metalloproteins / metabolism; Molecular Structure
Sources: Web Of Science, ORCID
Added: January 10, 2022

2020 journal article

Alternative Reactivity of Leucine 5-Hydroxylase Using an Olefin-Containing Substrate to Construct a Substituted Piperidine Ring

BIOCHEMISTRY, 59(21), 1961–1965.

Contriutors: L. Cha n, S. Milikisiyants n, M. Davidson n, S. Xue*, T. Smirnova n, A. Smirnov n, Y. Guo*, W. Chang n

MeSH headings : Alkenes / chemistry; Alkenes / metabolism; Leucine / chemistry; Leucine / metabolism; Mixed Function Oxygenases / chemistry; Mixed Function Oxygenases / metabolism; Molecular Conformation; Nostoc / enzymology; Substrate Specificity
Sources: Web Of Science, ORCID
Added: June 22, 2020

2020 article

An Effective Strategy to Introduce Carbon Isotopes by Simple Swaps of CO2

Cha, L., & Chang, W.-chen. (2020, December). TRENDS IN CHEMISTRY, Vol. 2, pp. 1040–1042.

By: L. Cha n & W. Chang n

Contriutors: L. Cha n & W. Chang n

Sources: Web Of Science, ORCID
Added: January 4, 2021

2020 journal article

Epoxidation Catalyzed by the Nonheme Iron(II)- and 2-Oxoglutarate-Dependent Oxygenase, AsqJ: Mechanistic Elucidation of Oxygen Atom Transfer by a Ferryl Intermediate

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 142(13), 6268–6284.

By: J. Li*, H. Liao*, Y. Tang*, J. Huang n, L. Cha n, T. Lin*, J. Lee*, I. Kurnikov* ...

Contriutors: J. Li*, H. Liao*, Y. Tang*, J. Huang n, L. Cha n, T. Lin*, J. Lee*, I. Kurnikov* ...

MeSH headings : Aspergillus nidulans / chemistry; Aspergillus nidulans / enzymology; Aspergillus nidulans / metabolism; Crystallography, X-Ray; Epoxy Compounds / chemistry; Epoxy Compounds / metabolism; Fungal Proteins / chemistry; Fungal Proteins / metabolism; Iron / chemistry; Iron / metabolism; Ketoglutaric Acids / metabolism; Models, Molecular; Oxygen / chemistry; Oxygen / metabolism; Oxygenases / chemistry; Oxygenases / metabolism
Sources: Web Of Science, ORCID
Added: May 26, 2020

2020 journal article

Pathway from N-Alkylglycine to Alkylisonitrile Catalyzed by Iron(II) and 2-Oxoglutarate-Dependent Oxygenases

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 59(19), 7367–7371.

Contriutors: T. Chen n, J. Chen*, Y. Tang*, J. Zhou*, Y. Guo* & W. Chang n

author keywords: catalysis; decarboxylation; enzymes; iron; reaction mechanisms
MeSH headings : Catalysis; Glycine / analogs & derivatives; Glycine / chemical synthesis; Hydroxylation; Iron / chemistry; Models, Molecular; Nitriles / chemical synthesis; Nuclear Magnetic Resonance, Biomolecular; Oxidation-Reduction; Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / chemistry; X-Ray Diffraction
Sources: Web Of Science, ORCID
Added: May 8, 2020

2019 journal article

A New Microbial Pathway for Organophosphonate Degradation Catalyzed by Two Previously Misannotated Non-Heme-Iron Oxygenases

BIOCHEMISTRY, 58(12), 1627–1647.

By: L. Rajkovich, M. Pandelia*, A. Mitchell*, W. Chang*, B. Zhang*, A. Boal*, C. Krebs*, J. Bollinger*

MeSH headings : Aminoethylphosphonic Acid / analogs & derivatives; Aminoethylphosphonic Acid / chemistry; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Escherichia coli / genetics; Humans; Iron / chemistry; Ketoglutaric Acids / chemistry; Organophosphonates; Organophosphorus Compounds / chemistry; Oxidation-Reduction; Oxygenases / chemistry; Oxygenases / genetics; Pseudomonas / enzymology; Rhodobacteraceae / enzymology; Substrate Specificity
Sources: Web Of Science, ORCID
Added: April 15, 2019

2019 journal article

Reaction Mechanism of a Nonheme Iron Enzyme Catalyzed Oxidative Cyclization via C-C Bond Formation

ORGANIC LETTERS, 21(1), 228–232.

By: W. Chang n, Z. Yang*, Y. Tu* & T. Chien*

Contriutors: W. Chang n, Z. Yang*, Y. Tu* & T. Chien*

MeSH headings : Biocatalysis; Cyclization; Molecular Conformation; Nonheme Iron Proteins / metabolism; Oxidation-Reduction; Podophyllotoxin / biosynthesis; Podophyllotoxin / chemistry; Stereoisomerism
Sources: Web Of Science, ORCID
Added: January 28, 2019

2019 journal article

Repurposing Nonheme Iron Hydroxylases To Enable Catalytic Nitrile Installation through an Azido Group Assistance

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 141(8), 3419–3423.

By: M. Davidson n, M. McNamee n, R. Fan*, Y. Guo* & W. Chang n

Contriutors: M. Davidson n, M. McNamee n, R. Fan*, Y. Guo* & W. Chang n

Sources: Web Of Science, ORCID
Added: March 25, 2019

2019 article

Unraveling flavoenzyme reaction mechanisms using flavin analogues and linear free energy relationships

NEW APPROACHES FOR FLAVIN CATALYSIS, Vol. 620, pp. 167–188.

By: C. Thibodeaux*, W. Chang n & H. Liu*

Contriutors: C. Thibodeaux*, W. Chang n & H. Liu*

MeSH headings : Enzyme Assays / methods; Enzymes / chemistry; Flavins / chemistry; Flavoproteins / chemistry; Kinetics; Oxidation-Reduction; Thermodynamics
Sources: Web Of Science, ORCID
Added: December 30, 2019

2018 journal article

Elucidating the Reaction Pathway of Decarboxylation-Assisted Olefination Catalyzed by a Mononuclear Non-Heme Iron Enzyme

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 140(45), 15190–15193.

Contriutors: C. Yu n, Y. Tang*, L. Cha n, S. Milikisiyants n, T. Smirnova n, A. Smirnov n, Y. Guo*, W. Chang n

MeSH headings : Alkenes / chemistry; Alkenes / metabolism; Biocatalysis; Decarboxylation; Molecular Structure; Nonheme Iron Proteins / metabolism
Sources: Web Of Science, ORCID
Added: December 10, 2018

2018 journal article

Insights into the Desaturation of Cyclopeptin and its C3 Epimer Catalyzed by a non-Heme Iron Enzyme: Structural Characterization and Mechanism Elucidation

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 57(7), 1831–1835.

By: H. Liao*, J. Li*, J. Huang n, M. Davidson n, I. Kurnikov*, T. Lin*, J. Lee*, M. Kurnikova* ...

Contriutors: H. Liao*, J. Li*, J. Huang n, M. Davidson n, I. Kurnikov*, T. Lin*, J. Lee*, M. Kurnikova* ...

author keywords: carbocations; C-C bond formation; desaturation; enzyme mechanisms; viridicatin
MeSH headings : Aspergillus nidulans / enzymology; Biocatalysis; Catalytic Domain; Cytochrome P-450 Enzyme System / metabolism; Epoxy Compounds / chemistry; Epoxy Compounds / metabolism; Ferric Compounds / chemistry; Fungal Proteins / chemistry; Fungal Proteins / metabolism; Ketoglutaric Acids / chemistry; Ketoglutaric Acids / metabolism; Molecular Dynamics Simulation; Peptides / chemistry; Peptides / metabolism; Quantum Theory; Stereoisomerism
Sources: Web Of Science, ORCID
Added: August 6, 2018

2018 journal article

Installation of the Ether Bridge of Lolines by the Iron- and 2-Oxoglutarate-Dependent Oxygenase, LolO: Regio- and Stereochemistry of Sequential Hydroxylation and Oxacyclization Reactions

Biochemistry, 57(14), 2074–2083.

MeSH headings : Alkaloids / chemistry; Epichloe / enzymology; Fungal Proteins / chemistry; Iron / chemistry; Ketoglutaric Acids / chemistry; Oxygenases / chemistry
Sources: Crossref, ORCID
Added: December 11, 2020

2018 journal article

Mechanistic Elucidation of Two Catalytically Versatile Iron(II)- and -Ketoglutarate-Dependent Enzymes: Cases Beyond Hydroxylation

COMMENTS ON INORGANIC CHEMISTRY, 38(4), 127–165.

By: W. Chang n, P. Liu* & Y. Guo*

Contriutors: W. Chang n, P. Liu* & Y. Guo*

author keywords: alpha-ketoglutarate-dependentenzyme; Ferryl intermediate; Endoperoxidation; Epoxidation; Desaturation
Sources: Web Of Science, ORCID
Added: October 29, 2018

2018 journal article

Mechanistic Investigation of Oxidative Decarboxylation Catalyzed by Two Iron(II)- and 2-Oxoglutarate-Dependent Enzymes

BIOCHEMISTRY, 57(12), 1838–1841.

By: J. Huang n, Y. Tang*, C. Yu n, D. Sanyal n, X. Jia n, X. Liu*, Y. Guo*, W. Chang n

Contriutors: J. Huang n, Y. Tang*, C. Yu n, D. Sanyal n, X. Jia n, X. Liu*, Y. Guo*, W. Chang n

MeSH headings : Carboxy-Lyases / chemistry; Catalysis; Iron / chemistry; Ketoglutaric Acids / chemistry
Sources: Web Of Science, ORCID
Added: August 6, 2018

2018 journal article

Structural Basis for Superoxide Activation of Flavobacterium johnsoniae Class i Ribonucleotide Reductase and for Radical Initiation by Its Dimanganese Cofactor

Biochemistry, 57(18), 2679–2693.

By: H. Rose*, M. Ghosh*, A. Maggiolo*, C. Pollock*, E. Blaesi*, V. Hajj*, Y. Wei*, L. Rajakovich* ...

Contriutors: H. Rose*, M. Ghosh*, A. Maggiolo*, C. Pollock*, E. Blaesi*, V. Hajj*, Y. Wei*, L. Rajakovich* ...

MeSH headings : Catalysis; Catalytic Domain; Flavobacterium / chemistry; Flavobacterium / enzymology; Flavoproteins / chemistry; Flavoproteins / metabolism; Iron / chemistry; Manganese / chemistry; Oxidation-Reduction; Oxygen / chemistry; Ribonucleotide Reductases / chemistry; Ribonucleotide Reductases / classification; Ribonucleotide Reductases / metabolism; Superoxides / chemistry; Tyrosine / chemistry
Sources: NC State University Libraries, ORCID
Added: August 6, 2018

2018 journal article

Substrate Conformation Correlates with the Outcome of Hyoscyamine 6β-Hydroxylase Catalyzed Oxidation Reactions

Journal of the American Chemical Society, 140(24), 7433–7436.

By: R. Ushimaru*, M. Ruszczycky*, W. Chang*, F. Yan*, Y. Liu* & H. Liu*

Contriutors: R. Ushimaru*, M. Ruszczycky*, W. Chang*, F. Yan*, Y. Liu* & H. Liu*

MeSH headings : Atropine Derivatives / chemistry; Catalysis; Cyclization; Hydroxylation; Mixed Function Oxygenases / chemistry; Models, Chemical; Molecular Conformation; Oxidation-Reduction
Sources: Crossref, ORCID
Added: December 11, 2020

2018 journal article

Two Distinct Mechanisms for C-C Desaturation by Iron(II)- and 2-(Oxo)glutarate-Dependent Oxygenases: Importance of alpha-Heteroatom Assistance

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 140(23), 7116–7126.

MeSH headings : Binding Sites; Deuterium / chemistry; Homoarginine / chemistry; Hydroxylation; Iron / chemistry; Ketoglutaric Acids / chemistry; Kinetics; Mixed Function Oxygenases / chemistry; Models, Chemical; Oxidation-Reduction; Stereoisomerism
Sources: Web Of Science, ORCID
Added: August 6, 2018

2017 journal article

In Vitro Stepwise Reconstitution of Amino Acid Derived Vinyl Isocyanide Biosynthesis: Detection of an Elusive Intermediate

ORGANIC LETTERS, 19(5), 1208–1211.

By: W. Chang n, D. Sanyal n, J. Huang n, K. Ittiamornkui, Q. Zhu* & X. Liu*

Contriutors: W. Chang n, D. Sanyal n, J. Huang n, K. Ittiamornkul*, Q. Zhu* & X. Liu*

MeSH headings : Amino Acids; Cyanides / chemistry; Molecular Structure
Sources: Web Of Science, ORCID
Added: August 6, 2018

2017 journal article

Structure-Guided Reprogramming of a Hydroxylase To Halogenate Its Small Molecule Substrate

BIOCHEMISTRY, 56(3), 441–444.

By: A. Mitchell, N. Dunham, J. Bergman, B. Wang, Q. Zhu*, W. Chang*, X. Liu*, A. Boal

Contriutors: A. Mitchell, N. Dunham, J. Bergman, B. Wang, Q. Zhu*, W. Chang*, X. Liu*, A. Boal

MeSH headings : Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Biocatalysis; Burkholderia / enzymology; Burkholderia / genetics; Gene Expression; Green Chemistry Technology; Halogenation; Halogens / chemistry; Halogens / metabolism; Iron / chemistry; Iron / metabolism; Ketoglutaric Acids / chemistry; Ketoglutaric Acids / metabolism; Mixed Function Oxygenases / chemistry; Mixed Function Oxygenases / genetics; Mixed Function Oxygenases / metabolism; Oxidoreductases / chemistry; Oxidoreductases / genetics; Oxidoreductases / metabolism; Protein Engineering; Structural Homology, Protein; Substrate Specificity; Succinic Acid / chemistry; Succinic Acid / metabolism
Sources: Web Of Science, ORCID
Added: August 6, 2018

2017 journal article

Visualizing the Reaction Cycle in an Iron(II)- and 2-(Oxo)-glutarate-Dependent Hydroxylase

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 139(39), 13830–13836.

By: A. Mitchell*, N. Dipham, R. Martinie*, J. Bergman*, C. Pollock*, K. Hu*, B. Allen*, W. Chang* ...

MeSH headings : Biocatalysis; Crystallography, X-Ray; Ferrous Compounds / chemistry; Ferrous Compounds / metabolism; Glutarates / chemistry; Glutarates / metabolism; Mixed Function Oxygenases / chemistry; Mixed Function Oxygenases / metabolism; Models, Molecular
Sources: Web Of Science, ORCID
Added: August 6, 2018

2016 journal article

Mechanistic Investigation of a Non-Heme Iron Enzyme Catalyzed Epoxidation in (-)-4 '-Methoxycyclopenin Biosynthesis

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 138(33), 10390–10393.

By: W. Chang n, J. Li*, J. Lee*, A. Cronican* & Y. Guo*

Contriutors: W. Chang n, J. Li*, J. Lee*, A. Cronican* & Y. Guo*

MeSH headings : Alkenes / chemistry; Aspergillus nidulans / enzymology; Benzodiazepinones / chemistry; Benzodiazepinones / metabolism; Biocatalysis; Enzymes / metabolism; Epoxy Compounds / chemistry; Iron; Kinetics; Oxygen / chemistry
Sources: Web Of Science, ORCID
Added: August 6, 2018

2016 journal article

Spectroscopic Evidence for the Two C–H-Cleaving Intermediates of Aspergillus nidulans Isopenicillin N Synthase

Journal of the American Chemical Society, 138(28), 8862–8874.

By: E. Tamanaha*, B. Zhang*, Y. Guo*, W. Chang*, E. Barr*, G. Xing*, J. St. Clair*, S. Ye* ...

Contriutors: E. Tamanaha*, B. Zhang*, Y. Guo*, W. Chang*, E. Barr*, G. Xing*, J. St Clair*, S. Ye* ...

MeSH headings : Aspergillus nidulans / enzymology; Kinetics; Oxidoreductases / chemistry; Oxidoreductases / metabolism; Oxygen / metabolism; Quantum Theory; Spectrum Analysis
Sources: ORCID, Crossref
Added: January 11, 2021

2015 journal article

Assembly of the unusual oxacycles in the orthosomycin antibiotics

Proceedings of the National Academy of Sciences, 112(39), 11989–11990.

By: A. Boal*, J. Bollinger* & W. Chang*

Contriutors: A. Boal*, J. Bollinger* & W. Chang*

MeSH headings : Aminoglycosides / chemistry; Anti-Bacterial Agents / chemistry; Oxygen / chemistry; Oxygenases / chemistry
Sources: ORCID, Crossref
Added: January 11, 2021

2015 journal article

Experimental correlation of substrate position with reaction outcome in the aliphatic halogenase, SyrB2

Journal of the American Chemical Society, 137(21), 6912–6919.

Contriutors: R. Martinie*, J. Livada*, W. Chang*, M. Green*, C. Krebs*, J. Bollinger*, A. Silakov*

MeSH headings : Bacterial Proteins / chemistry; Bacterial Proteins / metabolism; Molecular Conformation; Pseudomonas syringae / enzymology; Substrate Specificity
Source: ORCID
Added: January 11, 2021

2015 chapter

Mechanisms of 2-oxoglutarate-dependent oxygenases: The hydroxylation paradigm and beyond

In RSC Metallobiology: Vol. 2015-January (pp. 95–122).

By: J. Bollinger, W. Chang*, M. Matthews*, R. Martinie*, A. Boal & C. Krebs

Contriutors: J. Bollinger, W. Chang*, M. Matthews*, R. Martinie*, A. Boal & C. Krebs

Source: ORCID
Added: January 11, 2021

2015 journal article

Rapid Reduction of the Diferric-Peroxyhemiacetal Intermediate in Aldehyde-Deformylating Oxygenase by a Cyanobacterial Ferredoxin: Evidence for a Free-Radical Mechanism

Journal of the American Chemical Society, 137(36), 11695–11709.

MeSH headings : Acetals / chemistry; Aldehydes / chemistry; Cyanobacteria / chemistry; Electron Spin Resonance Spectroscopy; Ferredoxins / chemistry; Ferric Compounds / chemistry; Free Radicals / chemistry; Oxidation-Reduction; Oxygenases / chemistry; Spectroscopy, Mossbauer
Sources: ORCID, Crossref
Added: January 11, 2021

2014 journal article

Direct nitration and azidation of aliphatic carbons by an iron-dependent halogenase

Nature Chemical Biology, 10(3), 209–215.

By: M. Matthews*, W. Chang*, A. Layne*, L. Miles*, C. Krebs* & J. Bollinger*

Contriutors: M. Matthews*, W. Chang*, A. Layne*, L. Miles*, C. Krebs* & J. Bollinger*

MeSH headings : Azides / chemistry; Chromatography, Liquid; Coordination Complexes / chemistry; Enzymes / metabolism; Iron / chemistry; Ketoglutaric Acids / chemistry; Mass Spectrometry; Mutation; Nitrates / chemistry; Pseudomonas syringae / enzymology; Pseudomonas syringae / genetics
Sources: ORCID, Crossref
Added: January 11, 2021

2014 journal article

Mechanism of the C5 stereoinversion reaction in the biosynthesis of carbapenem antibiotics

Science, 343(6175), 1140–1144.

Contriutors: W. Chang*, Y. Guo*, C. Wang*, S. Butch*, A. Rosenzweig*, A. Boal*, C. Krebs*, J. Bollinger Jr.

MeSH headings : Carbapenems / biosynthesis; Carbapenems / chemistry; Catalysis; Crystallography, X-Ray; Enzymes / chemistry; Enzymes / genetics; Escherichia coli; Hydrogen / chemistry; Oxidation-Reduction; Pectobacterium carotovorum / enzymology; Stereoisomerism; Tyrosine / chemistry
Source: ORCID
Added: January 11, 2021

2014 journal article

Mechanistic Consequences of Chiral Radical Clock Probes: Analysis of the Mononuclear Non-Heme Iron Enzyme HppE with 2-Hydroxy-3-methylenecyclopropyl Radical Clock Substrates

Journal of the American Chemical Society, 136(8), 2944–2947.

By: H. Huang*, W. Chang*, G. Lin*, A. Romo*, P. Pai*, W. Russell*, D. Russell*, H. Liu*

Contriutors: H. Huang*, W. Chang*, G. Lin*, A. Romo*, P. Pai*, W. Russell*, D. Russell*, H. Liu*

MeSH headings : Fosfomycin / biosynthesis; Organophosphonates / chemistry; Oxidoreductases / chemistry; Stereoisomerism
Sources: ORCID, Crossref
Added: January 11, 2021

2013 journal article

Current development in isoprenoid precursor biosynthesis and regulation

Current Opinion in Chemical Biology, 17(4), 571–579.

By: W. Chang*, H. Song*, H. Liu* & P. Liu*

Contriutors: W. Chang*, H. Song*, H. Liu* & P. Liu*

MeSH headings : Animals; Biosynthetic Pathways; Hemiterpenes / biosynthesis; Hemiterpenes / chemistry; Humans; Mevalonic Acid / metabolism; Organophosphorus Compounds / chemistry; Terpenes / chemistry; Terpenes / metabolism
Sources: ORCID, Crossref
Added: January 11, 2021

2013 journal article

Evidence that the fosfomycin-producing epoxidase, HppE, is a non-heme-iron peroxidase

Science, 342(6161), 991–995.

Contriutors: C. Wang*, W. Chang*, Y. Guo*, H. Huang*, S. Peck*, M. Pandelia*, G. Lin*, H. Liu*, C. Krebs*, J. Bollinger Jr.

MeSH headings : Anti-Bacterial Agents / biosynthesis; Fosfomycin / biosynthesis; Hydrogen Peroxide / chemistry; Nonheme Iron Proteins / chemistry; Nonheme Iron Proteins / classification; Oxidoreductases / chemistry; Oxidoreductases / classification; Peroxidases / chemistry; Peroxidases / classification; Yersinia pseudotuberculosis / enzymology
Source: ORCID
Added: January 11, 2021

2013 journal article

Mechanistic studies of an unprecedented enzyme-catalysed 1,2-phosphono-migration reaction

Nature, 496(7443), 114–118.

By: W. Chang*, M. Dey*, P. Liu*, S. Mansoorabadi*, S. Moon*, Z. Zhao*, C. Drennan*, H. Liu*

Contriutors: W. Chang*, M. Dey*, P. Liu*, S. Mansoorabadi*, S. Moon*, Z. Zhao*, C. Drennan*, H. Liu*

MeSH headings : Biocatalysis; Biological Products / chemistry; Biological Products / metabolism; Crystallography, X-Ray; Fosfomycin / biosynthesis; Fosfomycin / chemistry; Fosfomycin / metabolism; Hydrogenation; Iron; Magnetic Resonance Spectroscopy; Models, Molecular; Nonheme Iron Proteins / chemistry; Nonheme Iron Proteins / metabolism; Organophosphonates / chemistry; Organophosphonates / metabolism; Oxidoreductases / chemistry; Oxidoreductases / metabolism; Substrate Specificity; Time Factors
Sources: ORCID, Crossref
Added: January 11, 2021

2013 book

Methylerythritol phosphate pathway of isoprenoid biosynthesis

In Annual Review of Biochemistry (Vol. 82, pp. 497–530).

By: L. Zhao*, W. Chang*, Y. Xiao*, H. Liu & P. Liu*

Contriutors: L. Zhao*, W. Chang*, Y. Xiao*, H. Liu & P. Liu*

author keywords: isoprenoids; biosynthesis; enzyme mechanism; metabolic engineering; synthetic biology; methylerythritol phosphate pathway; mevalonic acid pathway; MVA; MEP
MeSH headings : Biosynthetic Pathways / physiology; Catalysis; Erythritol / metabolism; Hemiterpenes / biosynthesis; Humans; Organophosphorus Compounds; Terpenes / metabolism
Source: ORCID
Added: January 11, 2021

2013 journal article

Reaction of HppE with Substrate Analogues: Evidence for Carbon–Phosphorus Bond Cleavage by a Carbocation Rearrangement

Journal of the American Chemical Society, 135(22), 8153–8156.

By: W. Chang*, S. Mansoorabadi* & H. Liu*

Contriutors: W. Chang*, S. Mansoorabadi* & H. Liu*

MeSH headings : Biocatalysis; Carbon / chemistry; Carbon / metabolism; Ketones / chemistry; Ketones / metabolism; Molecular Structure; Oxidoreductases / chemistry; Oxidoreductases / metabolism; Phosphorus / chemistry; Phosphorus / metabolism; Substrate Specificity
Sources: ORCID, Crossref
Added: January 11, 2021

2013 journal article

Substrate-Triggered Addition of Dioxygen to the Diferrous Cofactor of Aldehyde-Deformylating Oxygenase to Form a Diferric-Peroxide Intermediate

Journal of the American Chemical Society, 135(42), 15801–15812.

MeSH headings : Aldehyde-Lyases / chemistry; Aldehyde-Lyases / metabolism; Aldehydes / chemistry; Aldehydes / metabolism; Formates / chemistry; Formates / metabolism; Molecular Conformation; Nostoc / enzymology; Oxygen / chemistry; Oxygen / metabolism; Peroxides / chemistry; Peroxides / metabolism; Spectroscopy, Mossbauer; Substrate Specificity
Sources: ORCID, Crossref
Added: January 11, 2021

2012 journal article

Enzymatic Chemistry of Cyclopropane, Epoxide, and Aziridine Biosynthesis

Chemical Reviews, 112(3), 1681–1709.

By: C. Thibodeaux*, W. Chang* & H. Liu*

Contriutors: C. Thibodeaux*, W. Chang* & H. Liu*

MeSH headings : Animals; Aziridines / chemistry; Aziridines / metabolism; Cyclopropanes / chemistry; Cyclopropanes / metabolism; Enzymes / metabolism; Epoxy Compounds / chemistry; Epoxy Compounds / metabolism; Humans
Sources: ORCID, Crossref
Added: January 11, 2021

2012 journal article

Evidence for Only Oxygenative Cleavage of Aldehydes to Alk(a/e)nes and Formate by Cyanobacterial Aldehyde Decarbonylases

Biochemistry, 51(40), 7908–7916.

Contriutors: N. Li*, W. Chang*, D. Warui*, S. Booker*, C. Krebs* & J. Bollinger Jr.

MeSH headings : Aldehyde-Lyases / genetics; Aldehyde-Lyases / metabolism; Aldehydes / chemistry; Aldehydes / metabolism; Chromatography, Liquid; Cyanobacteria / enzymology; Formates / metabolism; Hexanes / chemistry; Hexanes / metabolism; Hydrolysis; Mass Spectrometry; Oxygen / chemistry; Oxygen / metabolism; Oxygen Isotopes / chemistry; Oxygen Isotopes / metabolism
Sources: ORCID, Crossref
Added: January 11, 2021

2012 journal article

Evidence for Radical-Mediated Catalysis by HppE: A Study Using Cyclopropyl and Methylenecyclopropyl Substrate Analogues

Journal of the American Chemical Society, 134(39), 16171–16174.

By: H. Huang*, W. Chang*, P. Pai*, A. Romo*, S. Mansoorabadi*, D. Russell*, H. Liu*

Contriutors: H. Huang*, W. Chang*, P. Pai*, A. Romo*, S. Mansoorabadi*, D. Russell*, H. Liu*

MeSH headings : Biocatalysis; Cyclopropanes / chemistry; Cyclopropanes / metabolism; Enzyme Activation; Free Radicals / chemistry; Free Radicals / metabolism; Oxidation-Reduction; Oxidoreductases / metabolism; Phosphorous Acids / chemistry; Phosphorous Acids / metabolism
Sources: ORCID, Crossref
Added: January 11, 2021

2012 journal article

Nucleophilic Participation of Reduced Flavin Coenzyme in Mechanism of UDP-galactopyranose Mutase

Journal of Biological Chemistry, 287(7), 4602–4608.

By: H. Sun*, M. Ruszczycky*, W. Chang*, C. Thibodeaux* & H. Liu*

Contriutors: H. Sun*, M. Ruszczycky*, W. Chang*, C. Thibodeaux* & H. Liu*

MeSH headings : Catalysis; Escherichia coli / enzymology; Escherichia coli / genetics; Escherichia coli Proteins / chemistry; Escherichia coli Proteins / genetics; Escherichia coli Proteins / metabolism; Flavin-Adenine Dinucleotide / analogs & derivatives; Flavin-Adenine Dinucleotide / chemistry; Flavin-Adenine Dinucleotide / genetics; Flavin-Adenine Dinucleotide / metabolism; Intramolecular Transferases / chemistry; Intramolecular Transferases / genetics; Intramolecular Transferases / metabolism; Kinetics
Sources: ORCID, Crossref
Added: January 11, 2021

2011 journal article

Mechanistic Studies of an IspH-Catalyzed Reaction: Implications for Substrate Binding and Protonation in the Biosynthesis of Isoprenoids

Angewandte Chemie International Edition, 50(51), 12304–12307.

By: W. Chang*, Y. Xiao*, H. Liu* & P. Liu*

Contriutors: W. Chang*, Y. Xiao*, H. Liu* & P. Liu*

author keywords: deoxyxylulose phosphate pathway; enzyme catalysis; isoprenoid biosynthesis; IspH enzyme; reaction mechanisms
MeSH headings : Binding Sites; Catalysis; Kinetics; Models, Molecular; Molecular Structure; Oxidoreductases / metabolism; Substrate Specificity; Terpenes / metabolism
Sources: ORCID, Crossref
Added: January 11, 2021

2011 journal article

Study of IspH, a Key Enzyme in the Methylerythritol Phosphate Pathway Using Fluoro-Substituted Substrate Analogues

Organic Letters, 13(21), 5912–5915.

By: Y. Xiao*, W. Chang*, H. Liu* & P. Liu*

Contriutors: Y. Xiao*, W. Chang*, H. Liu* & P. Liu*

MeSH headings : Alkyl and Aryl Transferases / metabolism; Biocatalysis; Erythritol / chemistry; Erythritol / metabolism; Fluorine Compounds / chemistry; Fluorine Compounds / metabolism; Models, Molecular; Molecular Structure; Oxidation-Reduction; Substrate Specificity; Sugar Phosphates / chemistry; Sugar Phosphates / metabolism
Sources: ORCID, Crossref
Added: January 11, 2021

2010 journal article

Linear Free Energy Relationships Demonstrate a Catalytic Role for the Flavin Mononucleotide Coenzyme of the Type II Isopentenyl Diphosphate:Dimethylallyl Diphosphate Isomerase

Journal of the American Chemical Society, 132(29), 9994–9996.

By: C. Thibodeaux*, W. Chang* & H. Liu*

Contriutors: C. Thibodeaux*, W. Chang* & H. Liu*

MeSH headings : Biocatalysis; Carbon-Carbon Double Bond Isomerases / chemistry; Carbon-Carbon Double Bond Isomerases / metabolism; Electrons; Flavin Mononucleotide / chemistry; Flavin Mononucleotide / metabolism; Hemiterpenes; Kinetics; Linear Models; Oxidation-Reduction; Staphylococcus aureus / enzymology; Thermodynamics
Sources: ORCID, Crossref
Added: January 11, 2021

2008 journal article

Evidence for the Involvement of Acid/Base Chemistry in the Reaction Catalyzed by the Type II Isopentenyl Diphosphate/Dimethylallyl Diphosphate Isomerase fromStaphylococcus aureus

Biochemistry, 47(8), 2547–2558.

By: C. Thibodeaux*, S. Mansoorabadi*, W. Kittleman*, W. Chang* & H. Liu*

Contriutors: C. Thibodeaux*, S. Mansoorabadi*, W. Kittleman*, W. Chang* & H. Liu*

MeSH headings : Acids / chemistry; Amines / chemistry; Carbon-Carbon Double Bond Isomerases / chemistry; Carbon-Carbon Double Bond Isomerases / metabolism; Catalysis; Flavin Mononucleotide / metabolism; Hemiterpenes; Hydrogen-Ion Concentration; Isoenzymes / chemistry; Isoenzymes / metabolism; Models, Biological; Spectrophotometry, Ultraviolet; Staphylococcus aureus / enzymology
Sources: ORCID, Crossref
Added: January 11, 2021

2008 journal article

Purification and Characterization of the Epoxidase Catalyzing the Formation of Fosfomycin from Pseudomonas syringae

Biochemistry, 47(33), 8726–8735.

By: J. Munos*, S. Moon*, S. Mansoorabadi*, W. Chang*, L. Hong*, F. Yan*, A. Liu*, H. Liu*

Contriutors: J. Munos*, S. Moon*, S. Mansoorabadi*, W. Chang*, L. Hong*, F. Yan*, A. Liu*, H. Liu*

MeSH headings : Amino Acid Sequence; Cloning, Molecular; Flavin Mononucleotide / chemistry; Flavin Mononucleotide / metabolism; Fosfomycin / biosynthesis; Gene Expression Regulation, Bacterial / physiology; Iron / chemistry; Iron / metabolism; Molecular Sequence Data; NAD / chemistry; NAD / metabolism; Oxidoreductases / chemistry; Oxidoreductases / isolation & purification; Oxidoreductases / metabolism; Oxygen / chemistry; Oxygen / metabolism; Pseudomonas syringae / enzymology
Sources: ORCID, Crossref
Added: January 11, 2021

Employment

Updated: September 1st, 2018 12:54

2016 - present

North Carolina State University Raleigh, NC, US
Chemistry

2011 - 2015

Pennsylvania State University University Park, PA, US

2003 - 2005

mandatory Military Service Kinmen , TW

Education

Updated: October 11th, 2019 13:50

2005 - 2011

University of Texas at Austin Austin, TX, US

1999 - 2003

National Taiwan University Taipei, TW