Wei-Chen Chang

Correction to “Regio- and Stereoselective Halogenation by an Iron(II)- and 2-Oxoglutarate-Dependent Halogenase in the Biosynthesis of Halogenated Nucleosides”

Palacios, P. M., Li, X., Rifayee, S. B. J. S., Tang, H., Karabencheva-Christova, T., Christov, C., … Guo, Y. (2026, January 24). Correction to “Regio- and Stereoselective Halogenation by an Iron(II)- and 2-Oxoglutarate-Dependent Halogenase in the Biosynthesis of Halogenated Nucleosides.” Journal of the American Chemical Society, Vol. 1.

An Alternative Mechanism for C–C Desaturation Underscores a Dual-Controlled Mechanism for the Fate of Radical Intermediate in Iron(II)- and 2-(Oxo)glutarate-Dependent Oxygenase DfmD

Zhang, X., Wang, L., Liu, J., Chen, T.-Y., Yan, S., Chang, W.-chen, … Wang, B. (2025, June 6). Journal of the American Chemical Society, Vol. 6.

Beyond the β–α–β Fold: Characterization of a SnoaL Domain in the Tautomerase Superfamily

Melkonian, T. R., Vuksanovic, N., Person, M. D., Chen, T.-Y., Chang, W.-chen, Allen, K. N., & Whitman, C. P. (2025, April 15). Biochemistry, Vol. 4.

Bioinformatic, structural, and biochemical analysis leads to the discovery of novel isonitrilases and decodes their substrate selectivity

Hostetler, T., Chen, T.-Y., & Chang, W.-C. (2025, January 1). RSC Chemical Biology, Vol. 1.

Comparison of a Nonheme Iron Cyclopropanase with a Homologous Hydroxylase Reveals Mechanistic Features Associated with Distinct Reaction Outcomes

Zheng, Y.-C., Li, X., Cha, L., Paris, J. C., Michael, C., Ushimaru, R., … Chang, W.-chen. (2025, February 4). Journal of the American Chemical Society, Vol. 2.

Discovery and Mechanism of a Diiron Enzyme in Ethylidene Azetidine Formation

Shen, Y., Zheng, Y.-C., Palacios, P. M., Wang, K., Maggiolo, A. O., Guo, Y., & Chang, W.-chen. (2025, October 13). Journal of the American Chemical Society, Vol. 10.

Discovery of Noncanonical Iron and 2-Oxoglutarate Dependent Enzymes Involved in C–C and C–N Bond Formation in Biosynthetic Pathways

Shen, Y., Sun, A., Guo, Y., & Chang, W.-chen. (2025, March 10). ACS Bio & Med Chem Au, Vol. 3.

Multiple Catalytic Branch Points in the Mechanism of Pyrrolidine Formation During Kainoid Biosynthesis Leads to Diverse Reaction Outcomes

Chen, T.-Y., Ruszczycky, M. W., Yao, A., Li, X., Chien, T.-C., & Chang, W.-chen. (2025, August 5). Journal of the American Chemical Society, Vol. 8.

Provision of a Redox Donor to Oxy-Ferrous PrnB Is Required for Pyrrolnitrin Synthesis

McMillan, R. B., Gering, H. E., Ledinina, A. E., Xue, S., Karandikar, S., Lin, S., … Makris, T. M. (2025, August 19). Biochemistry, Vol. 8.

Regio- and Stereoselective Halogenation by an Iron(II)- and 2-Oxoglutarate-Dependent Halogenase in the Biosynthesis of Halogenated Nucleosides

Palacios, P. M., Li, X., Rifayee, S. B. J. S., Tang, H., Karabencheva-Christova, T., Christov, C., … Guo, Y. (2025, December 16). Journal of the American Chemical Society.

Studies of Isonitrile Synthases Help Elucidate the Mechanism of Isonitrile Installation

Chen, T.-Y., Kim, W., Guo, M., Yao, A., Zhang, Y. J., & Chang, W.-chen. (2025, June 30). ACS Catalysis, Vol. 6.

Testing the Luedemann hypothesis: the discovery of novel antimicrobials from slow-growing microbes from nutrient-limited environments

Lin, B., Woo, S., Philbrick, A., Bacsa, J., Laskey, E., Mehra, N., … Luedemann, G. (2025, September 23). MSphere, Vol. 9.

The Mosher Method in the Study of Natural Products

Michael, C., Ruszczycky, M. W., & Chang, W.-chen. (2025, December 19). ACS in Focus.

A survey of C–C bond formation strategies and mechanism deployed by iron-containing enzymes in natural products

Michael, C., & Chang, W.-chen. (2024, June 1). Tetrahedron, Vol. 161.

New Frontiers in Nonheme Enzymatic Oxyferryl Species

Paris, J. C., Cheung, Y. H., Zhang, T., Chang, W., Liu, P., & Guo, Y. (2024, June 20). ChemBioChem, Vol. 8.

Optimized Substrate Positioning Enables Switches in the C–H Cleavage Site and Reaction Outcome in the Hydroxylation–Epoxidation Sequence Catalyzed by Hyoscyamine 6β-Hydroxylase

Wenger, E. S., Martinie, R. J., Ushimaru, R., Pollock, C. J., Sil, D., Li, A., … Bollinger, J. M. (2024, August 22). Journal of the American Chemical Society, Vol. 8.

Poly-γ-glutamylation of biomolecules

Bashiri, G., Bulloch, E. M. M., Bramley, W. R., Davidson, M., Stuteley, S. M., Young, P. G., … Squire, C. J. (2024, February 12). Nature Communications, Vol. 15.

Variation in Biosynthesis and Metal-Binding Properties of Isonitrile-Containing Peptides Produced by Mycobacteria versus Streptomyces

Chen, T.-Y., Chen, J., Ruszczycky, M. W., Hilovsky, D., Hostetler, T., Liu, X., … Chang, W.-chen. (2024, March 19). ACS Catalysis, Vol. 3.

A Ferric-Superoxide Intermediate Initiates P450-Catalyzed Cyclic Dipeptide Dimerization

Gering, H. E., Li, X., Tang, H., Swartz, P. D., Chang, W.-C., & Makris, T. M. (2023, August 23). Journal of the American Chemical Society, Vol. 8.

An S=1 Iron(IV) Intermediate Revealed in a Non‐Heme Iron Enzyme‐Catalyzed Oxidative C−S Bond Formation

Paris, J. C., Hu, S., Wen, A., Weitz, A. C., Cheng, R., Gee, L. B., … Guo, Y. (2023, August 28). Angewandte Chemie International Edition, Vol. 9.

Current understanding of lignan biosynthesis

Canty, N. K., & Chang, W.-chen. (2023, August 28). ARKIVOC.

Excision of a Protein-Derived Amine for p-Aminobenzoate Assembly by the Self-Sacrificial Heterobimetallic Protein CADD

Phan, H. N., Manley, O. M., Skirboll, S. S., Cha, L., Hilovsky, D., Chang, W.-chen, … Makris, T. M. (2023, November 7). Biochemistry, Vol. 62, pp. 3276–3282.

Mechanistic Analysis of Stereodivergent Nitroalkane Cyclopropanation Catalyzed by Nonheme Iron Enzymes

Ushimaru, R., Cha, L., Shimo, S., Li, X., Paris, J. C., Mori, T., … Abe, I. (2023, October 24). Journal of the American Chemical Society, Vol. 145, pp. 24210–24217.

Mechanistic Studies of Aziridine Formation Catalyzed by Mononuclear Non-Heme Iron Enzymes

Cha, L., Paris, J. C., Zanella, B., Spletzer, M., Yao, A., Guo, Y., & Chang, W.-chen. (2023, March 13). Journal of the American Chemical Society, Vol. 3.

Repurposing Iron‐ and 2‐Oxoglutarate‐Dependent Oxygenases to Catalyze Olefin Hydration

Wang, B., Lu, Y., Cha, L., Chen, T. Y., Palacios, P. M., Li, L., … Chen, C. (2023, August 28). Angewandte Chemie International Edition, Vol. 9.

Deciphering the Reaction Pathway of Mononuclear Iron Enzyme-Catalyzed N≡C Triple Bond Formation in Isocyanide Lipopeptide and Polyketide Biosynthesis

Chen, T.-Y., Zheng, Z., Zhang, X., Chen, J., Cha, L., Tang, Y., … Chang, W.-chen. (2022, January 31). ACS Catalysis, Vol. 2.

Elucidation of divergent desaturation pathways in the formation of vinyl isonitrile and isocyanoacrylate

Kim, W., Chen, T.-Y., Cha, L., Zhou, G., Xing, K., Canty, N. K., … Chang, W.-chen. (2022, September 12). Nature Communications, Vol. 9.

Mechanism of Methyldehydrofosmidomycin Maturation: Use Olefination to Enable Chain Elongation

Li, X., Xue, S., Guo, Y., & Chang, W.-chen. (2022, April 28). Journal of the American Chemical Society, Vol. 5.

Self-sacrificial tyrosine cleavage by an Fe:Mn oxygenase for the biosynthesis of para -aminobenzoate in Chlamydia trachomatis

Manley, O. M., Phan, H. N., Stewart, A. K., Mosley, D. A., Xue, S., Cha, L., … Makris, T. M. (2022, September 19). Proceedings of the National Academy of Sciences, Vol. 119.

A postreplicative C5-cytosine hypermodification triggered by bacteriophage methyltransferase and hydroxylase

Chen, T.-Y., & Chang, W.-chen. (2021, July 2). Proceedings of the National Academy of Sciences, Vol. 7.

BesC Initiates C–C Cleavage through a Substrate-Triggered and Reactive Diferric-Peroxo Intermediate

Manley, O. M., Tang, H., Xue, S., Guo, Y., Chang, W.-chen, & Makris, T. M. (2021, December 13). Journal of the American Chemical Society, Vol. 12.

Harnessing the Substrate Promiscuity of Dioxygenase AsqJ and Developing Efficient Chemoenzymatic Synthesis for Quinolones

Tang, H., Tang, Y., Kurnikov, I. V., Liao, H.-J., Chan, N.-L., Kurnikova, M. G., … Chang, W.-chen. (2021, June 6). ACS Catalysis, Vol. 6.

Identification of Cyclopropane Formation in the Biosyntheses of Hormaomycins and Belactosins: Sequential Nitration and Cyclopropanation by Metalloenzymes

Li, X., Shimaya, R., Dairi, T., Chang, W., & Ogasawara, Y. (2021, December 14). Angewandte Chemie International Edition, Vol. 12.

Mechanistic analysis of carbon–carbon bond formation by deoxypodophyllotoxin synthase

Tang, H., Wu, M.-H., Lin, H.-Y., Han, M.-R., Tu, Y.-H., Yang, Z.-J., … Chang, W.-chen. (2021, December 28). Proceedings of the National Academy of Sciences, Vol. 1.

Alternative Reactivity of Leucine 5-Hydroxylase Using an Olefin-Containing Substrate to Construct a Substituted Piperidine Ring

Cha, L., Milikisiyants, S., Davidson, M., Xue, S., Smirnova, T., Smirnov, A., … Chang, W.-chen. (2020, May 13). Biochemistry, Vol. 59, pp. 1961–1965.

An Effective Strategy to Introduce Carbon Isotopes by Simple Swaps of CO2

Cha, L., & Chang, W.-chen. (2020, October 23). Trends in Chemistry, Vol. 2, pp. 1040–1042.

Current Understanding toward Isonitrile Group Biosynthesis and Mechanism

Chen, T. Y., Chen, J., Tang, Y., Zhou, J., Guo, Y., & Chang, W. (2020, September 24). Chinese Journal of Chemistry, Vol. 39, pp. 463–472.

Deciphering Pyrrolidine and Olefin Formation Mechanism in Kainic Acid Biosynthesis

Chen, T.-Y., Xue, S., Tsai, W.-C., Chien, T.-C., Guo, Y., & Chang, W.-chen. (2020, December 18). ACS Catalysis, Vol. 12, pp. 278–282.

Epoxidation Catalyzed by the Nonheme Iron(II)- and 2-Oxoglutarate-Dependent Oxygenase, AsqJ: Mechanistic Elucidation of Oxygen Atom Transfer by a Ferryl Intermediate

Li, J., Liao, H.-J., Tang, Y., Huang, J.-L., Cha, L., Lin, T.-S., … Guo, Y. (2020, March 4). Journal of the American Chemical Society, Vol. 142, pp. 6268–6284.

Pathway from N‐Alkylglycine to Alkylisonitrile Catalyzed by Iron(II) and 2‐Oxoglutarate‐Dependent Oxygenases

Chen, T. Y., Chen, J., Tang, Y., Zhou, J., Guo, Y., & Chang, W. (2020, February 19). Angewandte Chemie International Edition, Vol. 59, pp. 7367–7371.

A New Microbial Pathway for Organophosphonate Degradation Catalyzed by Two Previously Misannotated Non-Heme-Iron Oxygenases

Rajakovich, L. J., Pandelia, M.-E., Mitchell, A. J., Chang, W.-chen, Zhang, B., Boal, A. K., … Bollinger, J. M. (2019, February 21). Biochemistry, Vol. 58, pp. 1627–1647.

Repurposing Nonheme Iron Hydroxylases To Enable Catalytic Nitrile Installation through an Azido Group Assistance

Davidson, M., McNamee, M., Fan, R., Guo, Y., & Chang, W.-chen. (2019, February 13). Journal of the American Chemical Society, Vol. 141, pp. 3419–3423.

Unraveling flavoenzyme reaction mechanisms using flavin analogues and linear free energy relationships

Thibodeaux, C. J., Chang, W.-chen, & Liu, H.-wen. (2019, January 1). Methods in Enzymology on CD-ROM/Methods in Enzymology, Vol. 620, pp. 167–188.

Elucidating the Reaction Pathway of Decarboxylation-Assisted Olefination Catalyzed by a Mononuclear Non-Heme Iron Enzyme

Yu, C.-P., Tang, Y., Cha, L., Milikisiyants, S., Smirnova, T. I., Smirnov, A. I., … Chang, W.-chen. (2018, October 30). Journal of the American Chemical Society, Vol. 140, pp. 15190–15193.

Insights into the Desaturation of Cyclopeptin and its C3 Epimer Catalyzed by a non‐Heme Iron Enzyme: Structural Characterization and Mechanism Elucidation

Liao, H. J., Li, J., Huang, J. L., Davidson, M., Kurnikov, I., Lin, T. S., … Chang, W. (2018, January 3). Angewandte Chemie International Edition, Vol. 57, pp. 1831–1835.

Installation of the Ether Bridge of Lolines by the Iron- and 2-Oxoglutarate-Dependent Oxygenase, LolO: Regio- and Stereochemistry of Sequential Hydroxylation and Oxacyclization Reactions

Biochemistry, 57(14), 2074–2083.

Mechanistic Elucidation of Two Catalytically Versatile Iron(II)- and α-Ketoglutarate-Dependent Enzymes: Cases Beyond Hydroxylation

Chang, W.-chen, Liu, P., & Guo, Y. (2018, July 4). Comments on Inorganic Chemistry, Vol. 38, pp. 127–165.

Mechanistic Investigation of Oxidative Decarboxylation Catalyzed by Two Iron(II)- and 2-Oxoglutarate-Dependent Enzymes

Huang, J.-L., Tang, Y., Yu, C.-P., Sanyal, D., Jia, X., Liu, X., … Chang, W.-chen. (2018, February 27). Biochemistry, Vol. 57, pp. 1838–1841.

Reaction Mechanism of a Nonheme Iron Enzyme Catalyzed Oxidative Cyclization via C–C Bond Formation

Chang, W.-chen, Yang, Z.-J., Tu, Y.-H., & Chien, T.-C. (2018, December 14). Organic Letters, Vol. 21, pp. 228–232.

Structural Basis for Superoxide Activation of Flavobacterium johnsoniae Class I Ribonucleotide Reductase and for Radical Initiation by Its Dimanganese Cofactor

Rose, H. R., Ghosh, M. K., Maggiolo, A. O., Pollock, C. J., Blaesi, E. J., Hajj, V., … Boal, A. K. (2018, April 2). Biochemistry, Vol. 57, pp. 2679–2693.

Substrate Conformation Correlates with the Outcome of Hyoscyamine 6β-Hydroxylase Catalyzed Oxidation Reactions

Journal of the American Chemical Society, 140(24), 7433–7436.

Two Distinct Mechanisms for C–C Desaturation by Iron(II)- and 2-(Oxo)glutarate-Dependent Oxygenases: Importance of α-Heteroatom Assistance

Dunham, N. P., Chang, W.-chen, Mitchell, A. J., Martinie, R. J., Zhang, B., Bergman, J. A., … Bollinger, J. M. (2018, April 30). Journal of the American Chemical Society, Vol. 140, pp. 7116–7126.

In Vitro Stepwise Reconstitution of Amino Acid Derived Vinyl Isocyanide Biosynthesis: Detection of an Elusive Intermediate

Chang, W.-chen, Sanyal, D., Huang, J.-L., Ittiamornkul, K., Zhu, Q., & Liu, X. (2017, February 17). Organic Letters, Vol. 19, pp. 1208–1211.

Visualizing the Reaction Cycle in an Iron(II)- and 2-(Oxo)-glutarate-Dependent Hydroxylase

Mitchell, A. J., Dunham, N. P., Martinie, R. J., Bergman, J. A., Pollock, C. J., Hu, K., … Boal, A. K. (2017, August 20). Journal of the American Chemical Society, Vol. 139, pp. 13830–13836.

Mechanistic Investigation of a Non-Heme Iron Enzyme Catalyzed Epoxidation in (−)-4′-Methoxycyclopenin Biosynthesis

Chang, W.-chen, Li, J., Lee, J. L., Cronican, A. A., & Guo, Y. (2016, July 21). Journal of the American Chemical Society, Vol. 138, pp. 10390–10393.

Spectroscopic Evidence for the Two C–H-Cleaving Intermediates of Aspergillus nidulans Isopenicillin N Synthase

Journal of the American Chemical Society, 138(28), 8862–8874.

Structure-Guided Reprogramming of a Hydroxylase To Halogenate Its Small Molecule Substrate

Mitchell, A. J., Dunham, N. P., Bergman, J. A., Wang, B., Zhu, Q., Chang, W.-chen, … Boal, A. K. (2016, December 28). Biochemistry, Vol. 56, pp. 441–444.

Assembly of the unusual oxacycles in the orthosomycin antibiotics

Proceedings of the National Academy of Sciences, 112(39), 11989–11990.

Experimental Correlation of Substrate Position with Reaction Outcome in the Aliphatic Halogenase, SyrB2

Martinie, R. J., Livada, J., Chang, W.-chen, Green, M. T., Krebs, C., Bollinger, J. M., & Silakov, A. (2015, May 12). Journal of the American Chemical Society, Vol. 137, pp. 6912–6919.

Mechanisms of 2-Oxoglutarate-Dependent Oxygenases: The Hydroxylation Paradigm and Beyond

Bollinger, J. M., Jr., Chang, W.-chen, Matthews, M. L., Martinie, R. J., Boal, A. K., & Krebs, C. (2015, April 23). RSC Metallobiology, pp. 95–122.

Rapid Reduction of the Diferric-Peroxyhemiacetal Intermediate in Aldehyde-Deformylating Oxygenase by a Cyanobacterial Ferredoxin: Evidence for a Free-Radical Mechanism

Journal of the American Chemical Society, 137(36), 11695–11709.

Direct nitration and azidation of aliphatic carbons by an iron-dependent halogenase

Nature Chemical Biology, 10(3), 209–215.

Mechanism of the C5 Stereoinversion Reaction in the Biosynthesis of Carbapenem Antibiotics

Chang, W.-chen, Guo, Y., Wang, C., Butch, S. E., Rosenzweig, A. C., Boal, A. K., … Bollinger, J. M. (2014, March 7). Science, Vol. 343, pp. 1140–1144.

Mechanistic Consequences of Chiral Radical Clock Probes: Analysis of the Mononuclear Non-Heme Iron Enzyme HppE with 2-Hydroxy-3-methylenecyclopropyl Radical Clock Substrates

Journal of the American Chemical Society, 136(8), 2944–2947.

Current development in isoprenoid precursor biosynthesis and regulation

Current Opinion in Chemical Biology, 17(4), 571–579.

Evidence that the Fosfomycin-Producing Epoxidase, HppE, Is a Non–Heme-Iron Peroxidase

Wang, C., Chang, W.-chen, Guo, Y., Huang, H., Peck, S. C., Pandelia, M. E., … Bollinger, J. M. (2013, October 11). Science, Vol. 342, pp. 991–995.

Mechanistic studies of an unprecedented enzyme-catalysed 1,2-phosphono-migration reaction

Nature, 496(7443), 114–118.

Methylerythritol Phosphate Pathway of Isoprenoid Biosynthesis

Zhao, L., Chang, W.-chen, Xiao, Y., Liu, H.-wen, & Liu, P. (2013, June 2). Annual Review of Biochemistry, Vol. 82, pp. 497–530.

Reaction of HppE with Substrate Analogues: Evidence for Carbon–Phosphorus Bond Cleavage by a Carbocation Rearrangement

Journal of the American Chemical Society, 135(22), 8153–8156.