Wei-Chen Chang

Comparison of a Nonheme Iron Cyclopropanase with a Homologous Hydroxylase Reveals Mechanistic Features Associated with Distinct Reaction Outcomes

Zheng, Y.-C., Li, X., Cha, L., Paris, J. C., Michael, C., Ushimaru, R., … Chang, W.-chen. (2025, February 4). JOURNAL OF THE AMERICAN CHEMICAL SOCIETY.

A survey of C-C bond formation strategies and mechanism deployed by iron-containing enzymes in natural products

TETRAHEDRON, 161.

Bioinformatic, structural, and biochemical analysis leads to the discovery of novel isonitrilases and decodes their substrate selectivity

Hostetler, T., Chen, T.-Y., & Chang, W.-chen. (2025, January 29). RSC CHEMICAL BIOLOGY, Vol. 1.

New Frontiers in Nonheme Enzymatic Oxyferryl Species

Paris, J. C., Cheung, Y. H., Zhang, T., Chang, W.-chen, Liu, P., & Guo, Y. (2024, August 7). CHEMBIOCHEM, Vol. 8.

Optimized Substrate Positioning Enables Switches in the C-H Cleavage Site and Reaction Outcome in the Hydroxylation-Epoxidation Sequence Catalyzed by Hyoscyamine 6<i>β</i>-Hydroxylase

Wenger, E. S., Martinie, R. J., Ushimaru, R., Pollock, C. J., Sil, D., Li, A., … Bollinger, J. M. (2024, August 22). JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol. 8.

Poly-γ-glutamylation of biomolecules

NATURE COMMUNICATIONS, 15(1).

Variation in Biosynthesis and Metal-Binding Properties of Isonitrile-Containing Peptides Produced by Mycobacteria versus Streptomyces

Chen, T.-Y., Chen, J., Ruszczycky, M. W., Hilovsky, D., Hostetler, T., Liu, X., … Chang, W.-chen. (2024, March 19). ACS CATALYSIS, Vol. 3.

A Ferric-Superoxide Intermediate Initiates P450-Catalyzed Cyclic Dipeptide Dimerization

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 8.

An <i>S</i>=1 Iron(IV) Intermediate Revealed in a Non-Heme Iron Enzyme-Catalyzed Oxidative C-S Bond Formation

Paris, J. C., Hu, S., Wen, A., Weitz, A. C., Cheng, R., Gee, L. B., … Guo, Y. (2023, September 13). ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol. 9.

Current understanding of lignan biosynthesis

[Review of ]. ARKIVOC.

Excision of a Protein-Derived Amine for <i>p</i>-Aminobenzoate Assembly by the Self-Sacrificial Heterobimetallic Protein CADD

BIOCHEMISTRY, 62(22), 3276–3282.

Mechanistic Analysis of Stereodivergent Nitroalkane Cyclopropanation Catalyzed by Nonheme Iron Enzymes

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 145(44), 24210–24217.

Mechanistic Studies of Aziridine Formation Catalyzed by Mononuclear Non-Heme Iron Enzymes

Cha, L., Paris, J. C., Zanella, B., Spletzer, M., Yao, A., Guo, Y., & Chang, W.-chen. (2023, March 13). JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol. 3.

Repurposing Iron- and 2-Oxoglutarate-Dependent Oxygenases to Catalyze Olefin Hydration

Wang, B., Lu, Y., Cha, L., Chen, T.-Y., Palacios, P. M., Li, L., … Chen, C. (2023, September 6). ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol. 9.

Deciphering the Reaction Pathway of Mononuclear Iron Enzyme-Catalyzed N C Triple Bond Formation in Isocyanide Lipopeptide and Polyketide Biosynthesis

ACS CATALYSIS, 12(4), 2270–2279.

Elucidation of divergent desaturation pathways in the formation of vinyl isonitrile and isocyanoacrylate

NATURE COMMUNICATIONS, 13(1).

Mechanism of Methyldehydrofosmidomycin Maturation: Use Olefination to Enable Chain Elongation

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 144(18), 8257–8266.

Mechanistic analysis of carbon-carbon bond formation by deoxypodophyllotoxin synthase

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 119(1).

Self-sacrificial tyrosine cleavage by an Fe:Mn oxygenase for the biosynthesis of para-aminobenzoate in Chlamydia trachomatis

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 119(39).

A postreplicative C5-cytosine hypermodification triggered by bacteriophage methyltransferase and hydroxylase

Chen, T.-Y., & Chang, W.-chen. (2021, July 13). PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol. 118.

BesC Initiates C-C Cleavage through a Substrate-Triggered and Reactive Diferric-Peroxo Intermediate

Manley, O. M., Tang, H., Xue, S., Guo, Y., Chang, W.-chen, & Makris, T. M. (2021, December 13). JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol. 12.

Current Understanding toward Isonitrile Group Biosynthesis and Mechanism

CHINESE JOURNAL OF CHEMISTRY, 39(2), 463–472.

Harnessing the Substrate Promiscuity of Dioxygenase AsqJ and Developing Efficient Chemoenzymatic Synthesis for Quinolones

ACS CATALYSIS, 11(12), 7186–7192.

Identification of Cyclopropane Formation in the Biosyntheses of Hormaomycins and Belactosins: Sequential Nitration and Cyclopropanation by Metalloenzymes

Li, X., Shimaya, R., Dairi, T., Chang, W.-chen, & Ogasawara, Y. (2021, December 27). ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol. 12.

Alternative Reactivity of Leucine 5-Hydroxylase Using an Olefin-Containing Substrate to Construct a Substituted Piperidine Ring

BIOCHEMISTRY, 59(21), 1961–1965.

An Effective Strategy to Introduce Carbon Isotopes by Simple Swaps of CO2

Cha, L., & Chang, W.-chen. (2020, December). TRENDS IN CHEMISTRY, Vol. 2, pp. 1040–1042.

Deciphering Pyrrolidine and Olefin Formation Mechanism in Kainic Acid Biosynthesis

ACS CATALYSIS, 11(1), 278–282.

Epoxidation Catalyzed by the Nonheme Iron(II)- and 2-Oxoglutarate-Dependent Oxygenase, AsqJ: Mechanistic Elucidation of Oxygen Atom Transfer by a Ferryl Intermediate

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 142(13), 6268–6284.

Pathway from N-Alkylglycine to Alkylisonitrile Catalyzed by Iron(II) and 2-Oxoglutarate-Dependent Oxygenases

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 59(19), 7367–7371.

A New Microbial Pathway for Organophosphonate Degradation Catalyzed by Two Previously Misannotated Non-Heme-Iron Oxygenases

BIOCHEMISTRY, 58(12), 1627–1647.

Reaction Mechanism of a Nonheme Iron Enzyme Catalyzed Oxidative Cyclization via C-C Bond Formation

ORGANIC LETTERS, 21(1), 228–232.

Repurposing Nonheme Iron Hydroxylases To Enable Catalytic Nitrile Installation through an Azido Group Assistance

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 141(8), 3419–3423.

Unraveling flavoenzyme reaction mechanisms using flavin analogues and linear free energy relationships

NEW APPROACHES FOR FLAVIN CATALYSIS, Vol. 620, pp. 167–188.

Elucidating the Reaction Pathway of Decarboxylation-Assisted Olefination Catalyzed by a Mononuclear Non-Heme Iron Enzyme

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 140(45), 15190–15193.

Insights into the Desaturation of Cyclopeptin and its C3 Epimer Catalyzed by a non-Heme Iron Enzyme: Structural Characterization and Mechanism Elucidation

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 57(7), 1831–1835.

Installation of the Ether Bridge of Lolines by the Iron- and 2-Oxoglutarate-Dependent Oxygenase, LolO: Regio- and Stereochemistry of Sequential Hydroxylation and Oxacyclization Reactions

Biochemistry, 57(14), 2074–2083.

Mechanistic Elucidation of Two Catalytically Versatile Iron(II)- and -Ketoglutarate-Dependent Enzymes: Cases Beyond Hydroxylation

COMMENTS ON INORGANIC CHEMISTRY, 38(4), 127–165.

Mechanistic Investigation of Oxidative Decarboxylation Catalyzed by Two Iron(II)- and 2-Oxoglutarate-Dependent Enzymes

BIOCHEMISTRY, 57(12), 1838–1841.

Structural Basis for Superoxide Activation of Flavobacterium johnsoniae Class i Ribonucleotide Reductase and for Radical Initiation by Its Dimanganese Cofactor

Biochemistry, 57(18), 2679–2693.

Substrate Conformation Correlates with the Outcome of Hyoscyamine 6β-Hydroxylase Catalyzed Oxidation Reactions

Journal of the American Chemical Society, 140(24), 7433–7436.

Two Distinct Mechanisms for C-C Desaturation by Iron(II)- and 2-(Oxo)glutarate-Dependent Oxygenases: Importance of alpha-Heteroatom Assistance

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 140(23), 7116–7126.

In Vitro Stepwise Reconstitution of Amino Acid Derived Vinyl Isocyanide Biosynthesis: Detection of an Elusive Intermediate

ORGANIC LETTERS, 19(5), 1208–1211.

Structure-Guided Reprogramming of a Hydroxylase To Halogenate Its Small Molecule Substrate

BIOCHEMISTRY, 56(3), 441–444.

Visualizing the Reaction Cycle in an Iron(II)- and 2-(Oxo)-glutarate-Dependent Hydroxylase

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 139(39), 13830–13836.

Mechanistic Investigation of a Non-Heme Iron Enzyme Catalyzed Epoxidation in (-)-4 '-Methoxycyclopenin Biosynthesis

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 138(33), 10390–10393.

Spectroscopic Evidence for the Two C–H-Cleaving Intermediates of Aspergillus nidulans Isopenicillin N Synthase

Journal of the American Chemical Society, 138(28), 8862–8874.

Assembly of the unusual oxacycles in the orthosomycin antibiotics

Proceedings of the National Academy of Sciences, 112(39), 11989–11990.

Experimental correlation of substrate position with reaction outcome in the aliphatic halogenase, SyrB2

Journal of the American Chemical Society, 137(21), 6912–6919.

Mechanisms of 2-oxoglutarate-dependent oxygenases: The hydroxylation paradigm and beyond

In RSC Metallobiology: Vol. 2015-January (pp. 95–122).

Rapid Reduction of the Diferric-Peroxyhemiacetal Intermediate in Aldehyde-Deformylating Oxygenase by a Cyanobacterial Ferredoxin: Evidence for a Free-Radical Mechanism

Journal of the American Chemical Society, 137(36), 11695–11709.

Direct nitration and azidation of aliphatic carbons by an iron-dependent halogenase

Nature Chemical Biology, 10(3), 209–215.

Mechanism of the C5 stereoinversion reaction in the biosynthesis of carbapenem antibiotics

Science, 343(6175), 1140–1144.

Mechanistic Consequences of Chiral Radical Clock Probes: Analysis of the Mononuclear Non-Heme Iron Enzyme HppE with 2-Hydroxy-3-methylenecyclopropyl Radical Clock Substrates

Journal of the American Chemical Society, 136(8), 2944–2947.

Current development in isoprenoid precursor biosynthesis and regulation

Current Opinion in Chemical Biology, 17(4), 571–579.

Evidence that the fosfomycin-producing epoxidase, HppE, is a non-heme-iron peroxidase

Science, 342(6161), 991–995.

Mechanistic studies of an unprecedented enzyme-catalysed 1,2-phosphono-migration reaction

Nature, 496(7443), 114–118.

Methylerythritol phosphate pathway of isoprenoid biosynthesis

In Annual Review of Biochemistry (Vol. 82, pp. 497–530).

Reaction of HppE with Substrate Analogues: Evidence for Carbon–Phosphorus Bond Cleavage by a Carbocation Rearrangement

Journal of the American Chemical Society, 135(22), 8153–8156.

Substrate-Triggered Addition of Dioxygen to the Diferrous Cofactor of Aldehyde-Deformylating Oxygenase to Form a Diferric-Peroxide Intermediate

Journal of the American Chemical Society, 135(42), 15801–15812.

Enzymatic Chemistry of Cyclopropane, Epoxide, and Aziridine Biosynthesis

Chemical Reviews, 112(3), 1681–1709.

Evidence for Only Oxygenative Cleavage of Aldehydes to Alk(a/e)nes and Formate by Cyanobacterial Aldehyde Decarbonylases

Biochemistry, 51(40), 7908–7916.

Evidence for Radical-Mediated Catalysis by HppE: A Study Using Cyclopropyl and Methylenecyclopropyl Substrate Analogues

Journal of the American Chemical Society, 134(39), 16171–16174.

Nucleophilic Participation of Reduced Flavin Coenzyme in Mechanism of UDP-galactopyranose Mutase

Journal of Biological Chemistry, 287(7), 4602–4608.

Mechanistic Studies of an IspH-Catalyzed Reaction: Implications for Substrate Binding and Protonation in the Biosynthesis of Isoprenoids

Angewandte Chemie International Edition, 50(51), 12304–12307.

Study of IspH, a Key Enzyme in the Methylerythritol Phosphate Pathway Using Fluoro-Substituted Substrate Analogues

Organic Letters, 13(21), 5912–5915.

Linear Free Energy Relationships Demonstrate a Catalytic Role for the Flavin Mononucleotide Coenzyme of the Type II Isopentenyl Diphosphate:Dimethylallyl Diphosphate Isomerase

Journal of the American Chemical Society, 132(29), 9994–9996.

Evidence for the Involvement of Acid/Base Chemistry in the Reaction Catalyzed by the Type II Isopentenyl Diphosphate/Dimethylallyl Diphosphate Isomerase fromStaphylococcus aureus

Biochemistry, 47(8), 2547–2558.

Purification and Characterization of the Epoxidase Catalyzing the Formation of Fosfomycin from Pseudomonas syringae

Biochemistry, 47(33), 8726–8735.