Will Smith

College of Engineering

Works (6)

Updated: January 23rd, 2026 04:00

2025 journal article

Developing a process of lentivirus purification from recombinant fluids using peptide affinity ligands

Bioengineering & Translational Medicine, 10(6).

By: E. Barbieri n, G. Mollica n, S. Sripada n, S. Shastry n, Y. Wu n, A. Minzoni n, W. Smith n, E. Wuestenhagen* ...

author keywords: affinity chromatography; lentivirus; membrane chromatography; peptide ligands; product polishing
topics (OpenAlex): Virus-based gene therapy research; CAR-T cell therapy research; Viral Infectious Diseases and Gene Expression in Insects
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: April 14, 2025

2025 journal article

Integrating Affinity Chromatography in the Platform Process for Adenovirus Purification

Biotechnology and Bioengineering, 122(7), 1780–1792.

By: Y. Wu n, E. Barbieri n, W. Smith n, A. Minzoni n, R. Kilgore n, W. Chu n, M. Daniele n , S. Menegatti n 

Contributors: Y. Wu n, E. Barbieri n, W. Smith n, A. Minzoni n, R. Kilgore n, W. Chu n, M. Daniele n , S. Menegatti n 

author keywords: adenovirus; affinity chromatography; biomanufacturing; bioseparations; gene therapy
topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; Viral gastroenteritis research and epidemiology
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: May 6, 2025

2025 article

Integrating affinity chromatography in the platform process for Adenovirus purification

Wu, Y., Barbieri, E., Smith, W. K., Minzoni, A., Kilgore, R. E., Chu, W., … Menegatti, S. (2025, April 5). (Vol. 4). Vol. 4.

By: Y. Wu n, E. Barbieri*, W. Smith n, A. Minzoni n, R. Kilgore n, W. Chu n, M. Daniele n , S. Menegatti n 

Contributors: Y. Wu n, E. Barbieri*, W. Smith n, A. Minzoni n, R. Kilgore n, W. Chu n, M. Daniele n , S. Menegatti n 

topics (OpenAlex): Viral Infectious Diseases and Gene Expression in Insects; Protein purification and stability; Virus-based gene therapy research
Sources: NC State University Libraries, NC State University Libraries, ORCID
Added: January 18, 2026

2024 article

Novel multimodal cation-exchange membrane for the purification of a single-chain variable fragment from Pichia pastoris supernatant

Pham, D. N., Linova, M. Y., Smith, W. K., Brown, H., Elhanafi, D., Fan, J., … Carbonell, R. G. (2024, February 1). Journal of Chromatography A.

By: D. Pham *, M. Linova *, W. Smith n, H. Brown n, D. Elhanafi n, J. Fan n, J. Lavoie n, J. Woodley *, R. Carbonell n

author keywords: Multimodal ligand; Membrane chromatography; Nonwoven membranes; Single-chain variable fragment (scFv); Pichia pastoris fermentation
MeSH headings : Single-Chain Antibodies; Pichia / metabolism; Saccharomycetales / metabolism; Fermentation; Recombinant Proteins / metabolism
topics (OpenAlex): Protein purification and stability; Monoclonal and Polyclonal Antibodies Research; Enzyme Catalysis and Immobilization; Membrane-based Ion Separation Techniques; Innovative Microfluidic and Catalytic Techniques Innovation
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2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: April 8, 2024

2023 journal article

Peptide ligands for the affinity purification of adeno‐associated viruses from HEK 293 cell lysates

Biotechnology and Bioengineering, 120(8), 2283–2300.

By: W. Chu n, S. Shastry n, E. Barbieri n, R. Prodromou n, P. Greback‐Clarke n, W. Smith n, B. Moore n, R. Kilgore n ...

Contributors: W. Chu n, S. Shastry n, E. Barbieri n, R. Prodromou n, P. Greback-Clarke n, W. Smith n, B. Moore n, . al.

author keywords: adeno-associated virus; affinity chromatography; HEK 293 lysate; peptide ligands; virus purification
MeSH headings : Humans; Dependovirus / genetics; HEK293 Cells; Ligands; Peptides / genetics; Peptides / metabolism; Amino Acid Sequence; Genetic Vectors
topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; RNA Interference and Gene Delivery
TL;DR: Peptide ligands that selectively capture AAVs and release them under mild conditions are introduced, affording high recovery, high recovery of host cell proteins (HCPs), and high transduction activity of the purified viruses. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: August 7, 2023

2023 article

The downstream bioprocess toolbox for therapeutic viral vectors

Kilgore, R., Minzoni, A., Shastry, S., Smith, W., Barbieri, E., Wu, Y., … Menegatti, S. (2023, September 9). Journal of Chromatography A, Vol. 1709.

By: R. Kilgore n, A. Minzoni n, S. Shastry n, W. Smith n, E. Barbieri n, Y. Wu n, J. LeBarre n, W. Chu n, J. O'Brien n, S. Menegatti n 

author keywords: Viral vectors; Gene therapy; Affinity ligands; Chromatography; Adsorbents; AAV
topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; Bacteriophages and microbial interactions
TL;DR: This study presents a comprehensive overview of the downstream bioprocess toolbox for viral vectors established in the last decade, and discusses present efforts and future directions contributing to the success of this promising class of biological medicines. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: October 30, 2023

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