Yong-Jun Goh

Works (35)

Updated: April 25th, 2025 12:45

2023 article

Lactobacillus acidophilus Expressing Murine Rotavirus VP8 and Mucosal Adjuvants Induce Virus-Specific Immune Responses

Gilfillan, D., Vilander, A. C., Pan, M., Goh, Y. J., Sarah, O. F., Feng, N., … Dean, G. A. (2023, November 28). Vaccines, Vol. 11.

By: D. Gilfillan*, A. Vilander*, M. Pan  n, Y. Goh n, O. Sarah n, N. Feng *, B. Fox *, C. Lang* ...

author keywords: rotavirus; Lactobacillus acidophilus; next-generation vaccine
topics (OpenAlex): Viral gastroenteritis research and epidemiology; Animal Virus Infections Studies; Viral Infections and Immunology Research
TL;DR: It is indicated that novel rLA constructs expressing VP8 can be successfully constructed and used to generate modest homotypic protection from rotavirus challenge in an adult murine model, indicating the potential for a probiotic next-generation vaccine construct against human rotav virus. (via Semantic Scholar)
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Added: February 1, 2024

2022 article

Genomic and epigenetic landscapes drive CRISPR-based genome editing inBifidobacterium

Pan, M., Morovic, W., Hidalgo-Cantabrana, C., Roberts, A., Walden, K. K. O., Goh, Y. J., & Barrangou, R. (2022, July 20). Proceedings of the National Academy of Sciences, Vol. 7.

By: M. Pan  n, W. Morovic *, C. Hidalgo-Cantabrana  n, A. Roberts n, K. Walden *, Y. Goh n, R. Barrangou n 

author keywords: CRISPR-Cas; bifidobacterium; genomics; epigenomics; probiotics
MeSH headings : Bifidobacterium / genetics; CRISPR-Cas Systems; Gene Editing / methods; Genome, Bacterial / genetics; Genomics; Humans; Probiotics
topics (OpenAlex): CRISPR and Genetic Engineering; Bacteriophages and microbial interactions; RNA and protein synthesis mechanisms
TL;DR: This study adapted existing CRISPR effectors and editing strategies to generate a variety of editing outcomes in B. animalis subsp. (via Semantic Scholar)
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Added: July 21, 2022

2021 article

In Vivo Transcriptome of Lactobacillus acidophilus and Colonization Impact on Murine Host Intestinal Gene Expression

Goh, Y. J., Barrangou, R., & Klaenhammer, T. R. (2021, January 25). (G. B. Huffnagle, Ed.). MBio, Vol. 12.

By: Y. Goh n, R. Barrangou n  & T. Klaenhammer n

Ed(s): G. Huffnagle

author keywords: Lactobacillus; acidophilus; gut adaptation; in vivo gene expression; mouse colonization; probiotic
MeSH headings : Animals; Bacterial Adhesion; Bacterial Proteins / genetics; Gene Expression Profiling; Host Microbial Interactions / genetics; Immunity; Intestines / microbiology; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / physiology; Mice; Probiotics / administration & dosage; Sequence Analysis, RNA; Signal Transduction; Transcriptome
topics (OpenAlex): Probiotics and Fermented Foods; Infant Nutrition and Health; Viral gastroenteritis research and epidemiology
TL;DR: Metagenomic analysis of NCFM populations postcolonization demonstrated the genomic stability of L. acidophilus as a gut transient and further established its safety as a probiotic and biotherapeutic delivery platform. (via Semantic Scholar)
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Added: January 26, 2021

2021 article

Portable CRISPR-Cas9 N System for Flexible Genome Engineering in Lactobacillus acidophilus, Lactobacillus gasseri, and Lactobacillus paracasei

Goh, Y. J., & Barrangou, R. (2021, January 5). (E. G. Dudley, Ed.). Applied and Environmental Microbiology, Vol. 87.

By: Y. Goh n & R. Barrangou n 

Ed(s): E. Dudley

author keywords: Lactobacillus acidophilus; CRISPR; Cas9; nickase; genome editing; probiotic; lactic acid bacteria; Lactobacillus
MeSH headings : CRISPR-Associated Protein 9; CRISPR-Cas Systems; Deoxyribonuclease I; Gene Editing / methods; Genome, Bacterial; Lactobacillus acidophilus / genetics; Lactobacillus gasseri / genetics; Lacticaseibacillus paracasei / genetics
topics (OpenAlex): CRISPR and Genetic Engineering; RNA and protein synthesis mechanisms; Bacteriophages and microbial interactions
TL;DR: This work describes the development of a lactobacillus CRISPR-based editing system for genome manipulations in three Lactobacilli species belonging to the lactic acid bacteria (LAB), which are commonly known for their long history of use in food fermentations and as indigenous members of healthy microbiotas and for their emerging roles in human and animal commercial health-promoting applications. (via Semantic Scholar)
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Added: February 26, 2021

2020 article

An 1,4-α-Glucosyltransferase Defines a New Maltodextrin Catabolism Scheme in Lactobacillus acidophilus

Andersen, S., Møller, M. S., Poulsen, J.-C. N., Pichler, M. J., Svensson, B., Leggio, L. L., … Hachem, M. A. (2020, May 21). Applied and Environmental Microbiology.

By: S. Andersen*, M. Møller *, J. Poulsen *, M. Pichler *, B. Svensson *, L. Leggio *, Y. Goh n, M. Hachem *

author keywords: carbohydrate metabolism; Lactobacillus; disproportionating enzyme; gut microbiota; maltooligosaccharides; microbiota; oligosaccharide 1,4-alpha-glucanotransferase; prebiotic; probiotic; starch
MeSH headings : Amino Acid Sequence; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Glycogen Debranching Enzyme System / chemistry; Glycogen Debranching Enzyme System / genetics; Glycogen Debranching Enzyme System / metabolism; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / metabolism; Polysaccharides / metabolism
topics (OpenAlex): Enzyme Production and Characterization; Probiotics and Fermented Foods; Microbial Metabolites in Food Biotechnology
TL;DR: The maltooligosaccharide (MOS) utilization locus in Lactobacillus acidophilus NCFM, a model for human small-intestine lactobacilli, is revealed to be a 1,4-α-glucosyltransferase that disproportionates MOS with a degree of polymerization of ≥2, with a preference for maltotriose. (via Semantic Scholar)
Source: Web Of Science
Added: August 24, 2020

2020 article

Comparative Analysis of Lactobacillus gasseri and Lactobacillus crispatus Isolated From Human Urogenital and Gastrointestinal Tracts

Pan, M., Hidalgo-Cantabrana, C., Goh, Y. J., Sanozky-Dawes, R., & Barrangou, R. (2020, January 22). Frontiers in Microbiology, Vol. 10.

By: M. Pan  n, C. Hidalgo-Cantabrana n, Y. Goh n, R. Sanozky-Dawes n & R. Barrangou n 

author keywords: probiotics; niche-specific adaptation; Lactobacillus; women's health; in vitro vaginal fluid model
topics (OpenAlex): Gut microbiota and health; Probiotics and Fermented Foods; Reproductive tract infections research
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Added: February 17, 2020

2020 article

Deletion of S-Layer Associated Ig-Like Domain Protein Disrupts the Lactobacillus acidophilus Cell Surface

Klotz, C., Goh, Y. J., Sarah, O. F., Johnson, B., & Barrangou, R. (2020, March 17). Frontiers in Microbiology, Vol. 11.

By: C. Klotz n, Y. Goh n, O. Sarah n, B. Johnson n & R. Barrangou n 

author keywords: Lactobacillus; probiotic; S-layer; cell surface; S-layer associated protein; Ig-like domain
topics (OpenAlex): Probiotics and Fermented Foods; Microbial Metabolites in Food Biotechnology; Gut microbiota and health
TL;DR: Identification of similar proteins in alternative bacterial species may help pinpoint next-generation host-adapted probiotic candidates. (via Semantic Scholar)
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Added: April 27, 2020

2019 article

Outcomes and characterization of chromosomal self-targeting by native CRISPR-Cas systems in Streptococcus thermophilus

Cañez, C., Selle, K., Goh, Y. J., & Barrangou, R. (2019, May 1). FEMS Microbiology Letters, Vol. 366.

By: C. Cañez n, K. Selle n, Y. Goh n & R. Barrangou n 

author keywords: CRISPR; lactic acid bacteria; Cas9; Streptococcus; genome editing
MeSH headings : Bacteriophages / genetics; CRISPR-Cas Systems; Chromosomes, Bacterial / genetics; Gene Editing; Genome, Bacterial; Homologous Recombination; Plasmids / genetics; Streptococcus thermophilus / genetics; Streptococcus thermophilus / virology
topics (OpenAlex): CRISPR and Genetic Engineering; RNA and protein synthesis mechanisms; Bacterial Genetics and Biotechnology
TL;DR: Results suggest that HR contributes to the plasticity and remodeling of bacterial genomes, leading to evasion of genome targeting by CRISPR-Cas systems. (via Semantic Scholar)
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Added: September 3, 2019

2018 article

Characterization and Repurposing of Type I and Type II CRISPR–Cas Systems in Bacteria

Hidalgo-Cantabrana, C., Goh, Y. J., & Barrangou, R. (2018, September 24). Journal of Molecular Biology, Vol. 431, pp. 21–33.

By: C. Hidalgo-Cantabrana n, Y. Goh n & R. Barrangou n 

author keywords: CRISPR-Cas systems; CRISPR characterization; endogenous systems; genome editing
MeSH headings : Archaea / genetics; Bacteria / genetics; CRISPR-Cas Systems / genetics; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; Gene Editing / methods; Plasmids / genetics; RNA, Guide, CRISPR-Cas Systems / genetics
topics (OpenAlex): CRISPR and Genetic Engineering; Insect symbiosis and bacterial influences; Bacterial Genetics and Biotechnology
TL;DR: There is a wide diversity of native CRISPR-Cas systems in many bacteria and most archaea that await functional characterization and repurposing for genome editing applications in prokaryotes. (via Semantic Scholar)
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Added: February 11, 2019

2018 article

Deletion-based escape of CRISPR-Cas9 targeting in Lactobacillus gasseri

Stout, E. A., Sanozky-Dawes, R., Goh, Y. J., Crawley, A. B., Klaenhammer, T. R., & Barrangou, R. (2018, July 19). Microbiology, Vol. 164, pp. 1098–1111.

By: E. Stout n, R. Sanozky-Dawes n, Y. Goh n, A. Crawley n, T. Klaenhammer n & R. Barrangou n 

author keywords: Lactobacillus; CRISPR; Cas; Cas9; interference; spacer
MeSH headings : CRISPR-Associated Protein 9 / metabolism; Clustered Regularly Interspaced Short Palindromic Repeats; Interspersed Repetitive Sequences; Lactobacillus gasseri / genetics; Mutation; Plasmids; Recombination, Genetic; Sequence Deletion; Transformation, Bacterial
topics (OpenAlex): CRISPR and Genetic Engineering; Bacteriophages and microbial interactions; Insect symbiosis and bacterial influences
TL;DR: It is shown that deletions of spacers within CRISPR arrays constitute a key escape mechanism to evadeCRISPR targeting, while preserving the functionality of the CRISpr-Cas system. (via Semantic Scholar)
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Added: December 31, 2018

2018 article

Harnessing CRISPR-Cas systems for precision engineering of designer probiotic lactobacilli

Goh, Y. J., & Barrangou, R. (2018, December 11). Current Opinion in Biotechnology, Vol. 56, pp. 163–171.

By: Y. Goh n & R. Barrangou n 

MeSH headings : CRISPR-Cas Systems / genetics; Drug Delivery Systems; Gene Editing; Genetic Engineering / methods; Lactobacillus / genetics; Probiotics / metabolism
topics (OpenAlex): CRISPR and Genetic Engineering; Transgenic Plants and Applications; Bacteriophages and microbial interactions
TL;DR: How the rising adoption of CRISPR-based technologies for prokaryotic engineering and tunable transcriptional regulation can be translated into next-generation development of probiotic lactobacilli with enhanced robustness and designer functionalities is discussed. (via Semantic Scholar)
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Added: June 17, 2019

2017 article

Lactobacillus acidophilus Metabolizes Dietary Plant Glucosides and Externalizes Their Bioactive Phytochemicals

Theilmann, M. C., Goh, Y. J., Nielsen, K. F., Klaenhammer, T. R., Barrangou, R., & Hachem, M. A. (2017, November 22). (E. Martens & M. J. McFall-Ngai, Eds.). MBio, Vol. 8.

By: M. Theilmann *, Y. Goh n, K. Nielsen *, T. Klaenhammer n, R. Barrangou n  & M. Hachem *

Ed(s): E. Martens & M. McFall-Ngai

author keywords: Lactobacillus; beta-glucoside; bioavailability; gut microbiota; phytochemical; polydatin; polyphenols; resveratrol; xenobiotic metabolism
MeSH headings : Biological Availability; Dietary Carbohydrates / metabolism; Gastrointestinal Microbiome / physiology; Glucosides / metabolism; Glucosides / pharmacology; Humans; Hydrolysis; Lactobacillus acidophilus / drug effects; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / growth & development; Lactobacillus acidophilus / metabolism; Phytochemicals / metabolism; Polyphenols / metabolism; Resveratrol; Stilbenes / metabolism
topics (OpenAlex): Probiotics and Fermented Foods; Microbial Metabolites in Food Biotechnology; Gut microbiota and health
TL;DR: This work demonstrates the growth of Lactobacillus acidophilus on mono- and diglucosyl dietary plant glycosides (PGs) possessing small aromatic aglycones and highlights an important role of lactobacilli in the bioconversion of dietary PG and presents a framework from which to derive molecular insights into their metabolism by members of the human gut microbiota. (via Semantic Scholar)
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Added: August 6, 2018

2017 article

An Extracellular Cell-Attached Pullulanase Confers Branched α-Glucan Utilization in Human Gut Lactobacillus acidophilus

Møller, M. S., Goh, Y. J., Rasmussen, K. B., Cypryk, W., Celebioglu, H. U., Klaenhammer, T. R., … Hachem, M. A. (2017, April 15). Applied and Environmental Microbiology.

By: M. Møller *, Y. Goh n, K. Rasmussen*, W. Cypryk *, H. Celebioglu *, T. Klaenhammer n, B. Svensson *, M. Hachem *

author keywords: glycoside hydrolase family 13; human gut microbiota; starch; starch binding modules
MeSH headings : Amylopectin / chemistry; Amylopectin / metabolism; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Enzyme Stability; Gastrointestinal Tract / microbiology; Glucans / chemistry; Glucans / metabolism; Glycoside Hydrolases / chemistry; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Humans; Hydrolysis; Kinetics; Lactobacillus acidophilus / enzymology; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / metabolism; Substrate Specificity
topics (OpenAlex): Enzyme Production and Characterization; Microbial Metabolites in Food Biotechnology; Food composition and properties
TL;DR: The pivotal role of debranching enzymes in the breakdown of starchy branched α-glucan oligomers by human gut lactobacilli exemplified by Lactobacillus acidophilus NCFM, which is one of the best-characterized strains used as probiotics, is highlighted. (via Semantic Scholar)
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Added: August 6, 2018

2017 article

Deletion of Lipoteichoic Acid Synthase Impacts Expression of Genes Encoding Cell Surface Proteins in Lactobacillus acidophilus

Selle, K., Goh, Y. J., Johnson, B. R., Sarah, O. F., Andersen, J. M., Barrangou, R., & Klaenhammer, T. R. (2017, April 11). Frontiers in Microbiology, Vol. 8.

By: K. Selle n, Y. Goh n, B. Johnson n, O. Sarah n, J. Andersen n, R. Barrangou n , T. Klaenhammer n

topics (OpenAlex): Probiotics and Fermented Foods; Gut microbiota and health; Bacterial Genetics and Biotechnology
TL;DR: Results elucidate the impact of ltaS deletion on the transcriptome and extracellular proteins of L. acidophilus, suggesting roles of LTA in cell morphology and ion homeostasis as a structural component of the Gram positive cell wall. (via Semantic Scholar)
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Added: August 6, 2018

2017 article

Investigating the Effect of Growth Phase on the Surface-Layer Associated Proteome of Lactobacillus acidophilus Using Quantitative Proteomics

Klotz, C., O'Flaherty, S., Goh, Y. J., & Barrangou, R. (2017, November 7). Frontiers in Microbiology, Vol. 8.

By: C. Klotz n, S. O'Flaherty n, Y. Goh n & R. Barrangou n 

author keywords: Lactobacillus; probiotic; cell surface; S-layer; quantitative proteomics
topics (OpenAlex): Probiotics and Fermented Foods; Bacteriophages and microbial interactions; Genomics and Phylogenetic Studies; Advanced Proteomics Techniques and Applications; Glycosylation and Glycoproteins Research
TL;DR: This study establishes a framework for quantifying condition-dependent changes to cell surface proteins that can easily be applied to other S-layer forming bacteria. (via Semantic Scholar)
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Added: August 6, 2018

2017 article

The S-layer Associated Serine Protease Homolog PrtX Impacts Cell Surface-Mediated Microbe-Host Interactions of Lactobacillus acidophilus NCFM

Johnson, B. R., Sarah, O. F., Goh, Y. J., Carroll, I., Barrangou, R., & Klaenhammer, T. R. (2017, June 30). Frontiers in Microbiology, Vol. 8.

By: B. Johnson n, O. Sarah n, Y. Goh n, I. Carroll *, R. Barrangou n  & T. Klaenhammer n

author keywords: serine protease; S-layer; S-layer associated proteins; Lactobacillus; probiotic; intestinal barrier integrity; mucin; fibronectin
topics (OpenAlex): Probiotics and Fermented Foods; Antimicrobial Peptides and Activities; Infant Nutrition and Health
TL;DR: The absence of PrtX within the exoproteome of a ΔprtX strain caused morphological changes, resulting in a pleiotropic increase of the organisms’ immunomodulatory properties and interactions with some intestinal epithelial cell components. (via Semantic Scholar)
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Added: August 6, 2018

2016 article

Differential proteome and cellular adhesion analyses of the probiotic bacterium Lactobacillus acidophilus NCFM grown on raffinose – an emerging prebiotic

Celebioglu, H. U., Ejby, M., Majumder, A., Købler, C., Goh, Y. J., Thorsen, K., … Svensson, B. (2016, March 9). PROTEOMICS.

By: H. Celebioglu *, M. Ejby *, A. Majumder*, C. Købler*, Y. Goh n, K. Thorsen *, B. Schmidt*, S. O'Flaherty n ...

author keywords: Adhesion; HT-29 cells; Moonlighting proteins; Probiotics; Raffinose; Surface and whole cell proteomes
MeSH headings : Bacterial Adhesion; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Galactose / metabolism; Gene Expression Regulation, Bacterial; Gene Ontology; Glucosyltransferases / genetics; Glucosyltransferases / metabolism; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; HT29 Cells; Humans; Lactobacillus acidophilus / drug effects; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / growth & development; Lactobacillus acidophilus / metabolism; Molecular Sequence Annotation; Peptide Elongation Factor G / genetics; Peptide Elongation Factor G / metabolism; Prebiotics; Probiotics / metabolism; Proteome / genetics; Proteome / metabolism; Raffinose / pharmacology; Staining and Labeling; alpha-Galactosidase / genetics; alpha-Galactosidase / metabolism
topics (OpenAlex): Probiotics and Fermented Foods; Microbial Metabolites in Food Biotechnology; Digestive system and related health; Gut microbiota and health
TL;DR: Insight at the molecular and cellular levels contributed to the understanding of the interplay of a synbiotic composed of NCFM and raffinose with the host. (via Semantic Scholar)
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Added: August 6, 2018

2015 article

SIGNR 3‐dependent immune regulation by Lactobacillus acidophilus surface layer protein A in colitis

Lightfoot, Y. L., Selle, K., Yang, T., Goh, Y. J., Sahay, B., Zadeh, M., … Mohamadzadeh, M. (2015, February 9). The EMBO Journal.

By: Y. Lightfoot *, K. Selle n, T. Yang *, Y. Goh n, B. Sahay *, M. Zadeh *, J. Owen*, N. Colliou* ...

author keywords: colitis; immune regulation; Lactobacillus acidophilus; SIGNR3; surface layer protein A
MeSH headings : Animals; Antigens, CD / genetics; Antigens, CD / immunology; Bacterial Proteins / genetics; Bacterial Proteins / immunology; Inflammatory Bowel Diseases / genetics; Inflammatory Bowel Diseases / immunology; Inflammatory Bowel Diseases / microbiology; Inflammatory Bowel Diseases / pathology; Intestinal Mucosa / immunology; Intestinal Mucosa / pathology; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / immunology; Lectins, C-Type / genetics; Lectins, C-Type / immunology; Lipopolysaccharides / genetics; Lipopolysaccharides / immunology; Mice; Mice, Knockout; Protein Binding / genetics; Protein Binding / immunology; Teichoic Acids / genetics; Teichoic Acids / immunology
topics (OpenAlex): Clostridium difficile and Clostridium perfringens research; Helicobacter pylori-related gastroenterology studies; Probiotics and Fermented Foods
TL;DR: This work presents critical insights into SlpA/SIGNR3‐induced responses that are integral to the potential development of novel biological therapies for autoinflammatory diseases, including IBD. (via Semantic Scholar)
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3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2015 article

Prebiotics: why definitions matter

Hutkins, R. W., Krumbeck, J. A., Bindels, L. B., Cani, P. D., Fahey, G., Goh, Y. J., … Sanders, M. E. (2015, September 29). Current Opinion in Biotechnology.

By: R. Hutkins *, J. Krumbeck *, L. Bindels *, P. Cani *, G. Fahey*, Y. Goh n, B. Hamaker *, E. Martens * ...

MeSH headings : Animals; Community Participation; Food Industry; Gastrointestinal Microbiome; Humans; Prebiotics
topics (OpenAlex): Microbial Metabolites in Food Biotechnology; Gut microbiota and health; Probiotics and Fermented Foods
TL;DR: Developments in community-wide sequencing and glycomics have revealed that more complex interactions occur between putative prebiotic substrates and the gut microbiota than previously considered. (via Semantic Scholar)
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Added: August 6, 2018

2014 article

Genetic Mechanisms of Prebiotic Oligosaccharide Metabolism in Probiotic Microbes

Goh, Y. J., & Klaenhammer, T. R. (2014, December 23). Annual Review of Food Science and Technology.

By: Y. Goh n & T. Klaenhammer n

author keywords: Lactobacillus; Bifidobacterium; fructooligosaccharide; galactooligosaccharide; prebiotic; probiotic
MeSH headings : Bifidobacterium / metabolism; Lactobacillus / metabolism; Oligosaccharides / metabolism; Prebiotics; Probiotics
topics (OpenAlex): Infant Nutrition and Health; Microbial Metabolites in Food Biotechnology; Gut microbiota and health
TL;DR: This review highlights recent findings on the genetic mechanisms involved in the utilization of prebiotic fructooligosaccharides, β-galactooligOSaccharide, human milk oligosac charides, and other prebiotics candidates by these probiotic microbes. (via Semantic Scholar)
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Added: August 6, 2018

2014 article

Insights into glycogen metabolism in Lactobacillus acidophilus: impact on carbohydrate metabolism, stress tolerance and gut retention

Goh, Y. J., & Klaenhammer, T. R. (2014, August 8). Microbial Cell Factories.

By: Y. Goh n & T. Klaenhammer n

author keywords: Lactobacilli; Glycogen; Probiotic; Raffinose; Gastrointestinal retention
MeSH headings : Adaptation, Physiological / drug effects; Animals; Carbohydrate Metabolism / drug effects; Carbon / pharmacology; Colony Count, Microbial; Gastrointestinal Tract / drug effects; Gastrointestinal Tract / microbiology; Glycogen / biosynthesis; Glycogen / metabolism; Humans; Lactobacillus acidophilus / drug effects; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / growth & development; Lactobacillus acidophilus / metabolism; Metabolic Networks and Pathways / drug effects; Metabolic Networks and Pathways / genetics; Mice; Models, Biological; Mutation / genetics; Operon / genetics; Prokaryotic Cells / drug effects; Prokaryotic Cells / metabolism; Raffinose / pharmacology; Species Specificity; Stress, Physiological / drug effects; Trehalose / pharmacology
topics (OpenAlex): Probiotics and Fermented Foods; Digestive system and related health; Infant Nutrition and Health
TL;DR: The present in vivo studies established the significance of glycogen biosynthesis on the competitive retention of L. acidophilus in the mouse intestinal tract, demonstrating for the first time that the ability to synthesize intracellular glycogen contributes to gut fitness and retention among probiotic microorganisms. (via Semantic Scholar)
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Added: August 6, 2018

2014 article

Recent insight in α-glucan metabolism in probiotic bacteria

Møller, M. S., Goh, Y. J., Viborg, A. H., Andersen, J. M., Klaenhammer, T. R., Svensson, B., & Hachem, M. A. (2014, May 3). Biologia.

By: M. Møller *, Y. Goh n, A. Viborg*, J. Andersen *, T. Klaenhammer n, B. Svensson *, M. Hachem *

author keywords: prebiotic; oligosaccharide uptake; ATP-binding cassette transport system; phosphotransferase system; transcriptional analysis; isomalto-oligosaccharide; malto-oligosaccharide
topics (OpenAlex): Enzyme Production and Characterization; Microbial Metabolites in Food Biotechnology; Food composition and properties
TL;DR: This review presents some aspects of α-glucan metabolism in probiotic bacteria and highlights vague issues that merit experimental effort, especially oligosaccharide uptake and the functionally unassigned enzymes, featuring in this important facet of glycan turnover by members of the gut microbiota. (via Semantic Scholar)
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Added: August 6, 2018

2014 article

Sortase-deficient lactobacilli: effect on immunomodulation and gut retention

Call, E. K., Goh, Y. J., Selle, K., Klaenhammer, T. R., & Sarah, O. F. (2014, December 13). Microbiology.

By: E. Call n, Y. Goh n, K. Selle n, T. Klaenhammer n & O. Sarah n

MeSH headings : Aminoacyltransferases / genetics; Aminoacyltransferases / immunology; Aminoacyltransferases / metabolism; Animals; Bacterial Adhesion; Bacterial Proteins / genetics; Bacterial Proteins / immunology; Bacterial Proteins / metabolism; Caco-2 Cells; Cysteine Endopeptidases / genetics; Cysteine Endopeptidases / immunology; Cysteine Endopeptidases / metabolism; Dendritic Cells / immunology; Dendritic Cells / microbiology; Gastrointestinal Tract / immunology; Gastrointestinal Tract / microbiology; Humans; Immunomodulation; Lactobacillus / enzymology; Lactobacillus / genetics; Lactobacillus / growth & development; Lactobacillus / physiology; Lactobacillus acidophilus / enzymology; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / growth & development; Lactobacillus acidophilus / physiology; Mice; Swine; Tumor Necrosis Factor-alpha / immunology
topics (OpenAlex): Biochemical and Structural Characterization; Probiotics and Fermented Foods; Biochemical Analysis and Sensing Techniques
TL;DR: It is shown for the first time that sortase-dependent proteins contribute to gut retention of probiotic microbes in the gastrointestinal tract. (via Semantic Scholar)
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Added: August 6, 2018

2013 article

A functional glycogen biosynthesis pathway in Lactobacillus acidophilus: expression and analysis of the glg operon

Goh, Y. J., & Klaenhammer, T. R. (2013, July 24). Molecular Microbiology.

By: Y. Goh n & T. Klaenhammer n

MeSH headings : 1,4-alpha-Glucan Branching Enzyme / genetics; 1,4-alpha-Glucan Branching Enzyme / metabolism; Bile / metabolism; Biosynthetic Pathways / genetics; Carbon / metabolism; Energy Metabolism; Gene Deletion; Gene Expression Regulation, Bacterial; Glucose / metabolism; Glycogen / biosynthesis; Glycogen Phosphorylase / genetics; Glycogen Phosphorylase / metabolism; Glycogen Synthase / genetics; Glycogen Synthase / metabolism; Humans; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / growth & development; Lactobacillus acidophilus / metabolism; Operon; Raffinose / metabolism; Trehalose / metabolism
topics (OpenAlex): Probiotics and Fermented Foods; Microbial Metabolites in Food Biotechnology; Digestive system and related health
TL;DR: The data indicate that glycogen metabolism is involved in growth maintenance, bile tolerance and complex carbohydrate utilization in L. acidophilus, a probiotic microbe in the human gastrointestinal environment. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2013 article

Identification of extracellular surface-layer associated proteins in Lactobacillus acidophilus NCFM

Johnson, B., Selle, K., Sarah, O. F., Goh, Y. J., & Klaenhammer, T. (2013, September 4). Microbiology.

By: B. Johnson n, K. Selle n, O. Sarah n, Y. Goh n & T. Klaenhammer n

MeSH headings : Animals; Cells, Cultured; Chromatography, Liquid; Dendritic Cells / drug effects; Lactobacillus acidophilus / chemistry; Lactobacillus acidophilus / immunology; Mass Spectrometry; Membrane Glycoproteins / analysis; Membrane Glycoproteins / immunology; Mice; Tumor Necrosis Factor-alpha / metabolism
topics (OpenAlex): Probiotics and Fermented Foods; Bacterial Genetics and Biotechnology; Antimicrobial Peptides and Activities
TL;DR: P phenotypic characterization of the deletion mutant demonstrated that the SLAP LBA1029 contributes to a pro-inflammatory TNF-α response from murine DCs, and appears to impart important surface display features and immunological properties to microbes that are coated by S-layers. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2013 article

Recent insight into oligosaccharide uptake and metabolism in probiotic bacteria

Hachem, M. A., Andersen, J. M., Barrangou, R., Møller, M. S., Fredslund, F., Majumder, A., … Svensson, B. (2013, August 1). Biocatalysis and Biotransformation, Vol. 31, pp. 226–235.

By: M. Hachem *, J. Andersen *, R. Barrangou n , M. Møller *, F. Fredslund *, A. Majumder*, M. Ejby *, S. Lahtinen* ...

author keywords: Prebiotic; oligosaccharide uptake; ATP-binding cassette transport system; phosphotransferase system; transcriptional analysis
topics (OpenAlex): Probiotics and Fermented Foods; Gut microbiota and health; Microbial Metabolites in Food Biotechnology; Food composition and properties
TL;DR: It is demonstrated for the first time that non-naturally occurring tri- and tetra-saccharides are internalized and efficiently utilized by probiotic bacteria in some cases better than well-established natural prebiotics. (via Semantic Scholar)
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10. Reduced Inequalities (OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2013 article

Transcriptional analysis of oligosaccharide utilization by Bifidobacterium lactisBl-04

Andersen, J. M., Barrangou, R., Hachem, M. A., Lahtinen, S. J., Goh, Y. J., Svensson, B., & Klaenhammer, T. R. (2013, May 10). BMC Genomics, Vol. 14, p. 312.

By: J. Andersen  n, R. Barrangou* , M. Hachem *, S. Lahtinen*, Y. Goh n, B. Svensson *, T. Klaenhammer n

author keywords: Bifidobacterium lactis; Transcriptomics; ABC transporter; GPH transporter; Prebiotics; Glycoside hydrolase
MeSH headings : Bifidobacterium / drug effects; Bifidobacterium / genetics; Cell Membrane / drug effects; Cell Membrane / metabolism; Data Mining; Gene Expression Profiling; Genetic Variation / genetics; Genomics; Multigene Family / genetics; Oligosaccharides / pharmacology; Prebiotics; Transcription, Genetic / drug effects; Up-Regulation / drug effects
topics (OpenAlex): Microbial Metabolites in Food Biotechnology; Digestive system and related health; Infant Nutrition and Health; Enzyme Production and Characterization; Biofuel production and bioconversion
TL;DR: This study identified the differential gene expression for utilization of potential prebiotics highlighting the extensive capabilities of Bifidobacterium lactis Bl-04 to utilize oligosaccharides. (via Semantic Scholar)
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3. Good Health and Well-being (Web of Science; OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2012 article

Transcriptional Analysis of Prebiotic Uptake and Catabolism by Lactobacillus acidophilus NCFM

Andersen, J. M., Barrangou, R., Hachem, M. A., Lahtinen, S. J., Goh, Y.-J., Svensson, B., & Klaenhammer, T. R. (2012, September 19). (C. Gibas, Ed.). PLoS ONE, Vol. 7, p. e44409.

By: J. Andersen  n, R. Barrangou* , M. Hachem *, S. Lahtinen*, Y. Goh n, B. Svensson *, T. Klaenhammer n

Ed(s): C. Gibas

MeSH headings : ATP-Binding Cassette Transporters / metabolism; Cellobiose / pharmacology; Gene Expression Regulation, Bacterial / drug effects; Glucans / pharmacology; Isomaltose / analogs & derivatives; Isomaltose / pharmacology; Lactobacillus acidophilus / drug effects; Lactobacillus acidophilus / enzymology; Lactobacillus acidophilus / metabolism; Oligosaccharides / pharmacology; Phosphoenolpyruvate Sugar Phosphotransferase System / metabolism; Prebiotics; Raffinose / pharmacology; Sugar Alcohols / pharmacology
topics (OpenAlex): Probiotics and Fermented Foods; Microbial Metabolites in Food Biotechnology; Digestive system and related health
TL;DR: Differential transcriptomics and functional genomics were used to identify genes in Lactobacillus acidophilus NCFM involved in the uptake and catabolism of 11 potential prebiotic compounds consisting of α- and β- linked galactosides and glucosides, highlighting the broad oligosaccharide metabolic repertoire of L. acidophillus NCFM. (via Semantic Scholar)
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Sources: Web Of Science, ORCID
Added: August 6, 2018

2011 article

Specialized adaptation of a lactic acid bacterium to the milk environment: the comparative genomics of Streptococcus thermophilus LMD-9

Goh, Y. J., Goin, C., Sarah, O. F., Altermann, E., & Hutkins, R. (2011, August 30). Microbial Cell Factories.

By: Y. Goh*, C. Goin*, O. Sarah n, E. Altermann * & R. Hutkins *

MeSH headings : Animals; Cattle; Computational Biology; Dairy Products / microbiology; Genomics; Humans; Lactic Acid / metabolism; Milk / microbiology; Streptococcus thermophilus / genetics; Streptococcus thermophilus / metabolism
topics (OpenAlex): Probiotics and Fermented Foods; Genomics and Phylogenetic Studies; Bacteriophages and microbial interactions
TL;DR: The genome of S. thermophilus LMD-9 is shaped by its domestication in the dairy environment, with gene features that conferred rapid growth in milk, stress response mechanisms and host defense systems that are relevant to its industrial applications. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2011 article

Transcriptional and functional analysis of galactooligosaccharide uptake bylacSinLactobacillus acidophilus

Andersen, J. M., Barrangou, R., Hachem, M. A., Lahtinen, S., Goh, Y. J., Svensson, B., & Klaenhammer, T. R. (2011, October 17). Proceedings of the National Academy of Sciences, Vol. 108, pp. 17785–17790.

By: J. Andersen  n, R. Barrangou* , M. Hachem *, S. Lahtinen, Y. Goh n, B. Svensson *, T. Klaenhammer n

author keywords: lactose permease; catabolite repression element
MeSH headings : DNA, Complementary / genetics; Gene Expression Regulation, Bacterial / physiology; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / metabolism; Membrane Transport Proteins / metabolism; Microarray Analysis; Oligonucleotide Array Sequence Analysis; Oligosaccharides / pharmacokinetics; Prebiotics; Reverse Transcriptase Polymerase Chain Reaction; beta-Galactosidase / genetics; beta-Galactosidase / metabolism
topics (OpenAlex): Probiotics and Fermented Foods; Microbial Metabolites in Food Biotechnology; Gut microbiota and health; Enzyme Production and Characterization
TL;DR: P phenotypic confirmation of the functional LacS permease not only for the utilization of lactose and GOS but also lactitol is confirmed, suggesting a prominent role of LacS in the metabolism of a broad range of prebiotic β-galactosides, known to selectively modulate the beneficial gut microbiota. (via Semantic Scholar)
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Sources: Web Of Science, ORCID
Added: August 6, 2018

2010 article

Functional Roles of Aggregation-Promoting-Like Factor in Stress Tolerance and Adherence of Lactobacillus acidophilus NCFM

Goh, Y. J., & Klaenhammer, T. R. (2010, June 21). Applied and Environmental Microbiology.

By: Y. Goh n & T. Klaenhammer n

MeSH headings : Adhesins, Bacterial / chemistry; Adhesins, Bacterial / genetics; Adhesins, Bacterial / metabolism; Anti-Bacterial Agents / toxicity; Bacterial Adhesion; Bile; Detergents / toxicity; Epithelial Cells / microbiology; Fibronectins / metabolism; Gastric Juice; Gene Deletion; Gene Expression Profiling; Lactobacillus acidophilus / drug effects; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / physiology; Microbial Viability / drug effects; Molecular Weight; Mucins / metabolism; Phylogeny; Protein Binding; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Amino Acid; Sodium Dodecyl Sulfate / toxicity
topics (OpenAlex): Probiotics and Fermented Foods; Infant Nutrition and Health; Digestive system and related health
TL;DR: R reverse transcriptase-quantitative PCR analysis revealed that the apf gene was highly induced during the stationary phase compared to that during the logarithmic phase, suggesting that the Apf-like proteins may contribute to the survival of L. acidophilus during transit through the digestive tract and, potentially, participate in the interactions with the host intestinal mucosa. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2009 article

Development and Application of a upp -Based Counterselective Gene Replacement System for the Study of the S-Layer Protein SlpX of Lactobacillus acidophilus NCFM

Goh, Y. J., Azcárate-Peril, M. A., O'Flaherty, S., Durmaz, E., Valence, F., Jardin, J., … Klaenhammer, T. R. (2009, March 21). Applied and Environmental Microbiology.

By: Y. Goh n, M. Azcárate-Peril n, S. O'Flaherty n, E. Durmaz n, F. Valence *, J. Jardin *, S. Lortal*, T. Klaenhammer n

MeSH headings : Anti-Bacterial Agents / pharmacology; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Drug Resistance, Bacterial; Fluorouracil / pharmacology; Gene Deletion; Genetic Complementation Test; Lactobacillus acidophilus / genetics; Membrane Glycoproteins / genetics; Molecular Sequence Data; Mutagenesis, Insertional / methods; Pentosyltransferases / genetics; Pentosyltransferases / metabolism; Plasmids; Recombination, Genetic; Selection, Genetic
topics (OpenAlex): Probiotics and Fermented Foods; Bacteriophages and microbial interactions; Bacterial Genetics and Biotechnology
TL;DR: Use of the upp-encoded uracil phosphoribosyltransferase (UPRTase) of L. acidophilus NCFM was explored as a counterselection marker to positively select for recombinants that have resolved from chromosomal integration of pORI-based plasmids. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2009 review

Genomic features of Lactobacillus species

[Review of ]. Frontiers in Bioscience, 14, 1362–1386.

By: Y. Goh & T. Klaenhammer

Source: NC State University Libraries
Added: August 6, 2018

2008 article

Analysis of the Genome Sequence ofLactobacillus gasseriATCC 33323 Reveals the Molecular Basis of an Autochthonous Intestinal Organism

Azcarate-Peril, M. A., Altermann, E., Goh, Y. J., Tallon, R., Sanozky-Dawes, R. B., Pfeiler, E. A., … Klaenhammer, T. R. (2008, June 7). Applied and Environmental Microbiology, Vol. 74, pp. 4610–4625.

By: M. Azcarate-Peril  n, E. Altermann n, Y. Goh n, R. Tallon n, R. Sanozky-Dawes n, E. Pfeiler n, S. O'Flaherty n, B. Buck n ...

MeSH headings : Adult; Bacterial Adhesion / genetics; Bacterial Adhesion / physiology; Bacterial Proteins / genetics; Child; DNA Primers; Gastrointestinal Tract / microbiology; Genes, Bacterial; Genome, Bacterial; Humans; Infant, Newborn; Intestines / microbiology; Lactobacillus / genetics; Lactobacillus / isolation & purification; Lactobacillus / pathogenicity; Open Reading Frames
topics (OpenAlex): Probiotics and Fermented Foods; Microbial Metabolites in Food Biotechnology; Gut microbiota and health
TL;DR: The results from this study indicated high intraspecies variability from a genome encoding traits important for survival and retention in the gastrointestinal tract. (via Semantic Scholar)
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UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2008 article

Functional Genomics of Probiotic Lactobacilli

Klaenhammer, T. R., Altermann, E., Pfeiler, E., Buck, B. L., Goh, Y.-J., O'Flaherty, S., … Duong, T. (2008, September 1). Journal of Clinical Gastroenterology, Vol. 42, pp. S160–S162.

By: T. Klaenhammer n, E. Altermann n, E. Pfeiler n, B. Buck n, Y. Goh n, S. O'Flaherty n, R. Barrangou n , T. Duong n

author keywords: Lactobacillus acidophilus; Lactobacillus johnsonii; Lactobacillus plantarum; Lactobacillus gasseri; genomics; probiotic cultures; lactic acid bacteria
MeSH headings : Animals; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Evolution, Molecular; Genome, Bacterial; Genomics; Humans; Lactobacillus / classification; Lactobacillus / genetics; Lactobacillus / metabolism; Lactobacillus / physiology; Probiotics / metabolism; Species Specificity
topics (OpenAlex): Probiotics and Fermented Foods; Microbial Metabolites in Food Biotechnology; Gut microbiota and health
TL;DR: A comparative genomic analysis of 4 completely sequenced Lactobacillus strains versus 25 lactic acid bacterial genomes present in the public database at thetime of analysis is conducted. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

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