Yuxuan Wu

College of Engineering

Works (6)

Updated: January 23rd, 2026 04:00

2025 journal article

Developing a process of lentivirus purification from recombinant fluids using peptide affinity ligands

Bioengineering & Translational Medicine, 10(6).

By: E. Barbieri n, G. Mollica n, S. Sripada n, S. Shastry n, Y. Wu n, A. Minzoni n, W. Smith n, E. Wuestenhagen* ...

author keywords: affinity chromatography; lentivirus; membrane chromatography; peptide ligands; product polishing
topics (OpenAlex): Virus-based gene therapy research; CAR-T cell therapy research; Viral Infectious Diseases and Gene Expression in Insects
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Sources: Web Of Science, NC State University Libraries, Crossref
Added: April 14, 2025

2025 journal article

Integrating Affinity Chromatography in the Platform Process for Adenovirus Purification

Biotechnology and Bioengineering, 122(7), 1780–1792.

By: Y. Wu n, E. Barbieri n, W. Smith n, A. Minzoni n, R. Kilgore n, W. Chu n, M. Daniele n , S. Menegatti n 

Contributors: Y. Wu n, E. Barbieri n, W. Smith n, A. Minzoni n, R. Kilgore n, W. Chu n, M. Daniele n , S. Menegatti n 

author keywords: adenovirus; affinity chromatography; biomanufacturing; bioseparations; gene therapy
topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; Viral gastroenteritis research and epidemiology
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: May 6, 2025

2025 article

Integrating affinity chromatography in the platform process for Adenovirus purification

Wu, Y., Barbieri, E., Smith, W. K., Minzoni, A., Kilgore, R. E., Chu, W., … Menegatti, S. (2025, April 5). (Vol. 4). Vol. 4.

By: Y. Wu n, E. Barbieri*, W. Smith n, A. Minzoni n, R. Kilgore n, W. Chu n, M. Daniele n , S. Menegatti n 

Contributors: Y. Wu n, E. Barbieri*, W. Smith n, A. Minzoni n, R. Kilgore n, W. Chu n, M. Daniele n , S. Menegatti n 

topics (OpenAlex): Viral Infectious Diseases and Gene Expression in Insects; Protein purification and stability; Virus-based gene therapy research
Sources: NC State University Libraries, NC State University Libraries, ORCID
Added: January 18, 2026

2025 article

Purification of CRISPR Cas12a from E. coli cell lysates using peptide affinity ligands

Wu, Y., Kilgore, R., Hetzler, Z., Chu, W., Moore, B., Barbieri, E., … Menegatti, S. (2025, May 17). Journal of Chromatography A, Vol. 1755.

By: Y. Wu n, R. Kilgore n, Z. Hetzler n, W. Chu n, B. Moore n, E. Barbieri n, Q. Wei n , S. Menegatti n 

author keywords: CRISPR Cas12a; Gene editing; Peptide ligands; Affinity chromatography; Protein purification
MeSH headings : Escherichia coli / chemistry; Escherichia coli / genetics; Escherichia coli / enzymology; Chromatography, Affinity / methods; Ligands; CRISPR-Associated Proteins / isolation & purification; CRISPR-Associated Proteins / metabolism; CRISPR-Associated Proteins / chemistry; Peptides / chemistry; Peptides / metabolism; CRISPR-Cas Systems; Bacterial Proteins / isolation & purification; Bacterial Proteins / metabolism; Bacterial Proteins / chemistry; Endodeoxyribonucleases / isolation & purification; Endodeoxyribonucleases / metabolism; Endodeoxyribonucleases / chemistry; Acidaminococcus / enzymology
topics (OpenAlex): CRISPR and Genetic Engineering; RNA and protein synthesis mechanisms; Advanced biosensing and bioanalysis techniques
Sources: Web Of Science, NC State University Libraries
Added: June 9, 2025

2024 journal article

Peptide ligands for the affinity purification of adenovirus from HEK293 and vero cell lysates

Journal of Chromatography A, 1736, 465396.

By: Y. Wu n, E. Barbieri n, R. Kilgore n, B. Moore n, W. Chu n, G. Mollica n, M. Daniele n , S. Menegatti n 

Contributors: Y. Wu n, E. Barbieri n, R. Kilgore n, B. Moore n, W. Chu n, G. Mollica n, M. Daniele n , S. Menegatti n 

author keywords: Affinity chromatography; Adenovirus; Viral vectors; Gene therapy; Peptide ligands
MeSH headings : Humans; Chromatography, Affinity / methods; Chlorocebus aethiops; Animals; Ligands; Vero Cells; HEK293 Cells; Peptides / chemistry; Peptides / isolation & purification; Peptides / metabolism; Adenoviridae / chemistry; Adenoviridae / isolation & purification; Capsid Proteins / isolation & purification; Capsid Proteins / metabolism; Capsid Proteins / chemistry
topics (OpenAlex): Monoclonal and Polyclonal Antibodies Research; Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects
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UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID, Crossref
Added: October 14, 2024

2023 article

The downstream bioprocess toolbox for therapeutic viral vectors

Kilgore, R., Minzoni, A., Shastry, S., Smith, W., Barbieri, E., Wu, Y., … Menegatti, S. (2023, September 9). Journal of Chromatography A, Vol. 1709.

By: R. Kilgore n, A. Minzoni n, S. Shastry n, W. Smith n, E. Barbieri n, Y. Wu n, J. LeBarre n, W. Chu n, J. O'Brien n, S. Menegatti n 

author keywords: Viral vectors; Gene therapy; Affinity ligands; Chromatography; Adsorbents; AAV
topics (OpenAlex): Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects; Bacteriophages and microbial interactions
TL;DR: This study presents a comprehensive overview of the downstream bioprocess toolbox for viral vectors established in the last decade, and discusses present efforts and future directions contributing to the success of this promising class of biological medicines. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: October 30, 2023

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