Zach Hetzler

College of Engineering

Works (8)

Updated: June 14th, 2025 05:01

2025 article

Purification of CRISPR Cas12a from E. coli cell lysates using peptide affinity ligands

Wu, Y., Kilgore, R., Hetzler, Z., Chu, W., Moore, B., Barbieri, E., … Menegatti, S. (2025, May 17). Journal of Chromatography A, Vol. 1755.

By: Y. Wu n, R. Kilgore n, Z. Hetzler n, W. Chu n, B. Moore n, E. Barbieri n, Q. Wei n , S. Menegatti n 

author keywords: CRISPR Cas12a; Gene editing; Peptide ligands; Affinity chromatography; Protein purification
MeSH headings : Escherichia coli / chemistry; Escherichia coli / genetics; Escherichia coli / enzymology; Chromatography, Affinity / methods; Ligands; CRISPR-Associated Proteins / isolation & purification; CRISPR-Associated Proteins / metabolism; CRISPR-Associated Proteins / chemistry; Peptides / chemistry; Peptides / metabolism; CRISPR-Cas Systems; Bacterial Proteins / isolation & purification; Bacterial Proteins / metabolism; Bacterial Proteins / chemistry; Endodeoxyribonucleases / isolation & purification; Endodeoxyribonucleases / metabolism; Endodeoxyribonucleases / chemistry; Acidaminococcus / enzymology
topics (OpenAlex): CRISPR and Genetic Engineering; RNA and protein synthesis mechanisms; Advanced biosensing and bioanalysis techniques
Sources: Web Of Science, NC State University Libraries
Added: June 9, 2025

2024 article

Adeno-associated virus genome quantification with amplification-free CRISPR-Cas12a

Hetzler, Z., Marinakos, S. M., Lott, N., Mohammad, N., Lass-Napiorkowska, A., Kolbe, J., … Wei, Q. (2024, March 25). Gene Therapy, Vol. 3.

By: Z. Hetzler n, S. Marinakos, N. Lott n, N. Mohammad n , A. Lass-Napiorkowska *, J. Kolbe n, L. Turrentine n, D. Fields n ...

MeSH headings : Dependovirus / genetics; CRISPR-Cas Systems; Humans; Genome, Viral; Genetic Vectors / genetics; HEK293 Cells
topics (OpenAlex): CRISPR and Genetic Engineering; Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects
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Sources: Web Of Science, NC State University Libraries
Added: April 15, 2024

2024 article

Liquid Metal‐Based Biosensors: Fundamentals and Applications

Jamalzadegan, S., Kim, S., Mohammad, N., Koduri, H., Hetzler, Z., Lee, G., … Wei, Q. (2024, January 23). Advanced Functional Materials, Vol. 1.

By: S. Jamalzadegan n, S. Kim n , N. Mohammad n , H. Koduri n, Z. Hetzler n, G. Lee n, M. Dickey n , Q. Wei n 

author keywords: biosensors; liquid metals; wearable sensors; soft materials; precision health; point-of-care
topics (OpenAlex): Advanced Sensor and Energy Harvesting Materials; Nanomaterials and Printing Technologies
TL;DR: The recent progress of LM‐based biosensors for personalized healthcare and disease monitoring via both physical and biochemical signaling is survey and the challenges and future directions of LM‐integrated biosensor platforms is discussed. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 12, 2024

2024 article

Ratiometric nonfluorescent CRISPR assay utilizing Cas12a-induced plasmid supercoil relaxation

Mohammad, N., Talton, L., Dalgan, S., Hetzler, Z., Steksova, A., & Wei, Q. (2024, June 8). Communications Chemistry, Vol. 7.

By: N. Mohammad n , L. Talton n, S. Dalgan n, Z. Hetzler n, A. Steksova n & Q. Wei n 

topics (OpenAlex): CRISPR and Genetic Engineering; Advanced biosensing and bioanalysis techniques; RNA and protein synthesis mechanisms
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: June 24, 2024

2023 article

Low-rate smartphone videoscopy for microsecond luminescence lifetime imaging with machine learning

Wang, Y., Sadeghi, S., Velayati, A., Paul, R., Hetzler, Z., Danilov, E., … Wei, Q. (2023, September 27). (R. Reis, Ed.). PNAS Nexus, Vol. 2.

By: Y. Wang  n, S. Sadeghi n, A. Velayati n, R. Paul  n, Z. Hetzler n, E. Danilov n , F. Ligler*, Q. Wei n 

Ed(s): R. Reis

author keywords: smartphone videoscopy; V-chopper; time-resolved; lifetime imaging; machine learning
topics (OpenAlex): Lanthanide and Transition Metal Complexes; Optical Imaging and Spectroscopy Techniques; Advanced MRI Techniques and Applications
TL;DR: A cost-effective and miniaturized smartphone lifetime imaging system integrated with a pulsed ultraviolet (UV) light-emitting diode (LED) for 2D luminescence lifetime imaging using a videoscopy-based virtual chopper (V-chopper) mechanism combined with machine learning. (via Semantic Scholar)
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7. Affordable and Clean Energy (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: November 6, 2023

2023 article

Single-use biosensors for biomanufacturing: Perspective on the state-of-the-art

Hetzler, Z., Lott, N., Poonam, A. D., Dalgan, S., & Wei, Q. (2023, October 31). Current Opinion in Biomedical Engineering, Vol. 28.

By: Z. Hetzler n, N. Lott n, A. Poonam n, S. Dalgan n & Q. Wei n 

author keywords: Biomanufacturing; Gene therapy; Process monitoring; Process analytical technology (PAT); Single-use sensors
topics (OpenAlex): Viral Infectious Diseases and Gene Expression in Insects; 3D Printing in Biomedical Research; bioluminescence and chemiluminescence research
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: January 2, 2024

2023 article

Unidirectional trans-cleaving behavior of CRISPR-Cas12a unlocks for an ultrasensitive assay using hybrid DNA reporters containing a 3′ toehold

Mohammad, N., Talton, L., Hetzler, Z., Gongireddy, M., & Wei, Q. (2023, August 31). Nucleic Acids Research, Vol. 8.

By: N. Mohammad n , L. Talton n, Z. Hetzler n, M. Gongireddy n & Q. Wei n 

topics (OpenAlex): CRISPR and Genetic Engineering; Advanced biosensing and bioanalysis techniques; RNA Interference and Gene Delivery
TL;DR: This study not only unfolded new insight into the trans-cleavage behavior of Cas12a but also demonstrated a sensitive CRISPR-Cas12a assay by using a hybrid dsDNA reporter molecule. (via Semantic Scholar)
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: September 18, 2023

2022 article

Flexible sensor patch for continuous carbon dioxide monitoring

Hetzler, Z., Wang, Y., Krafft, D., Jamalzadegan, S., Overton, L., Kudenov, M. W., … Wei, Q. (2022, September 27). Frontiers in Chemistry, Vol. 10.

By: Z. Hetzler n, Y. Wang n, D. Krafft n, S. Jamalzadegan n, L. Overton n, M. Kudenov n , F. Ligler*, Q. Wei n 

author keywords: CO2; in-line sensor; flexible sensor; process analytical technology; cell culture; biomanufacturing
topics (OpenAlex): Analytical Chemistry and Sensors; Gas Sensing Nanomaterials and Sensors; Advanced Chemical Sensor Technologies
Sources: Web Of Science, NC State University Libraries
Added: March 6, 2023

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