Golden Leaf Biomanufacturing Training and Education Center

Recent Works

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Sorted by most recent date added to the index first, which may not be the same as publication date order.

2026 article

Non‐Functionalized Graphene as an Electrical Sensing Surface for Bacterial Detection

Berthe, J., Giancaterino, S., Castillo, L., Boi, C., & Barbosa, S. (2026, February 13). Advanced Materials Interfaces.

By: J. Berthe*, S. Giancaterino*, L. Castillo*, C. Boi n & S. Barbosa*

co-author countries: Argentina 🇦🇷 Italy 🇮🇹
topics (OpenAlex): Graphene and Nanomaterials Applications; Polydiacetylene-based materials and applications; Biosensors and Analytical Detection
Source: ORCID
Added: February 19, 2026

2026 article

Adaptive Machine Learning Framework for Optimizing the Affinity Purification of Adeno‐Associated Viral Vectors

Idanwekhai, K. P., Shastry, S., Hurst, M. R., Minzoni, A., Barbieri, E., Remmler, L., … Tropsha, A. (2026, January 19). Biotechnology and Bioengineering, Vol. 1.

By: K. Idanwekhai*, S. Shastry n, M. Hurst n, A. Minzoni n, E. Barbieri*, L. Remmler*, E. Muratov, M. Daniele n, S. Menegatti n, A. Tropsha

co-author countries: Spain 🇪🇸

Contributors: K. Idanwekhai*, S. Shastry n, M. Hurst n, A. Minzoni n, E. Barbieri*, L. Remmler*, E. Muratov, . al.

topics (OpenAlex): Protein purification and stability; Virus-based gene therapy research; Viral Infectious Diseases and Gene Expression in Insects
Sources: NC State University Libraries, NC State University Libraries, ORCID
Added: January 21, 2026

2025 article

Engineering thermphilic enzymes of a synthetic rTCA cycle to increase carbon fixation in plants

Rose, R., Grunden, A., Buhrman, G., & Katta, V. (2025, January 1). SSRN Electronic Journal, Vol. 1.

By: R. Rose n, A. Grunden n, G. Buhrman n & V. Katta

topics (OpenAlex): Photosynthetic Processes and Mechanisms; Metalloenzymes and iron-sulfur proteins; Enzyme Catalysis and Immobilization
Sources: NC State University Libraries, NC State University Libraries
Added: January 27, 2026

2025 article

Production and characterization of redox enzymes from Methylorubrum extorquens and Paenibacilllus polymyxa for use in enzyme immobilization and electrochemical applications

Hunter, C., Rondon-Berrio, V., Buhrman, G., Crook, N., & Grunden, A. (2025, January 1). SSRN Electronic Journal, Vol. 1.

By: C. Hunter n, V. Rondon-Berrio n, G. Buhrman n, N. Crook n & A. Grunden n

topics (OpenAlex): Electrochemical sensors and biosensors
Sources: NC State University Libraries, NC State University Libraries
Added: January 27, 2026

2025 article

CAD design of a 3D structure made by individual fibers for immersion precipitation 3D printing

Boi, C., Onesti, R., & Malagoli, G. (2025, December 18). Zenodo (CERN European Organization for Nuclear Research), Vol. 12.

By: C. Boi n, R. Onesti* & G. Malagoli*

co-author countries: Italy 🇮🇹
topics (OpenAlex):
Sources: NC State University Libraries, NC State University Libraries
Added: January 17, 2026

2025 article

CAD design of a 3D structure made by individual fibers for immersion precipitation 3D printing

Boi, C., Onesti, R., & Malagoli, G. (2025, December 18). Zenodo (CERN European Organization for Nuclear Research), Vol. 12.

By: C. Boi n, R. Onesti* & G. Malagoli*

co-author countries: Italy 🇮🇹
topics (OpenAlex):
Sources: NC State University Libraries, NC State University Libraries
Added: January 17, 2026

2025 article

Scanning Electron Microscopy (SEM) images showing the internal and external morphology of 3D structures and individual fibers composing the structures fabricated via immersion precipitation 3D printing (ip3D printing)

Onesti, R., Boi, C., & Malagoli, G. (2025, December 18). Zenodo (CERN European Organization for Nuclear Research), Vol. 12.

By: R. Onesti*, C. Boi n & G. Malagoli*

co-author countries: Italy 🇮🇹
topics (OpenAlex):
Sources: NC State University Libraries, NC State University Libraries
Added: January 17, 2026

2025 article

Scanning Electron Microscopy (SEM) images showing the internal and external morphology of 3D structures and individual fibers composing the structures fabricated via immersion precipitation 3D printing (ip3D printing)

Onesti, R., Boi, C., & Malagoli, G. (2025, December 18). Zenodo (CERN European Organization for Nuclear Research), Vol. 12.

By: R. Onesti*, C. Boi n & G. Malagoli*

co-author countries: Italy 🇮🇹
topics (OpenAlex):
Sources: NC State University Libraries, NC State University Libraries
Added: January 17, 2026

2025 article

G-code for the production of 3D structures made by individual filaments through immersion precipitation 3D printing

Malagoli, G., Boi, C., & Onesti, R. (2025, December 18). Zenodo (CERN European Organization for Nuclear Research), Vol. 12.

By: G. Malagoli*, C. Boi n & R. Onesti n

co-author countries: Italy 🇮🇹
topics (OpenAlex):
Sources: NC State University Libraries, NC State University Libraries
Added: January 15, 2026

2025 article

G-code for the production of 3D structures made by individual filaments through immersion precipitation 3D printing

Malagoli, G., Boi, C., & Onesti, R. (2025, December 18). Zenodo (CERN European Organization for Nuclear Research), Vol. 12.

By: G. Malagoli*, C. Boi n & R. Onesti n

co-author countries: Italy 🇮🇹
topics (OpenAlex):
Sources: NC State University Libraries, NC State University Libraries
Added: January 15, 2026

2025 article

Comparison of anion-exchange chromatography matrices for purification of linear and supercoiled plasmid in a direct lysate workflow

Stoops, M., Shastry, S., Martinez-Mata, C., Elhanafi, D., & Pancorbo, J. (2025, October 24). Journal of Chromatography A.

topics (OpenAlex): Protein purification and stability; Viral Infectious Diseases and Gene Expression in Insects; Monoclonal and Polyclonal Antibodies Research
Source: NC State University Libraries
Added: November 22, 2025

2025 article proceedings

Machine Learning-Enabled Immunosensor for Label-Free Quantification of Virus Titers

By: R. Shukla n, H. Sun n, J. Wang n, J. Twiddy n, S. Shastry n, S. Menegatti n, M. Daniele n

Contributors: R. Shukla n, H. Sun n, J. Wang n, J. Twiddy n, S. Shastry n, S. Menegatti n, M. Daniele n

topics (OpenAlex): Advanced Biosensing Techniques and Applications; Biosensors and Analytical Detection; Advanced biosensing and bioanalysis techniques; Virus-based gene therapy research
Sources: NC State University Libraries, NC State University Libraries, ORCID, Crossref
Added: November 19, 2025

2025 article

Nanofiltration efficiency in the purification of lactose from ultrafiltered acid whey

Onesti, R., Roselli, M., Boi, C., & Bandini, S. (2025, October 29). Journal of Food Engineering.

By: R. Onesti*, M. Roselli*, C. Boi n & S. Bandini*

co-author countries: Italy 🇮🇹
topics (OpenAlex): Membrane Separation Technologies; Electrohydrodynamics and Fluid Dynamics; Electrospun Nanofibers in Biomedical Applications; Membrane-based Ion Separation Techniques; Extraction and Separation Processes
Source: ORCID
Added: November 1, 2025

2025 article

Flow-through polishing of Protein A eluates: Removal of high and low molecular weight impurities by size-exclusion-mixed-mode chromatography

LeBarre, J. P., Chu, W., Zhang, K., Bhandari, D., Boi, C., Carbonell, R., … Menegatti, S. (2025, September 12). Journal of Chromatography A.

By: J. LeBarre n, W. Chu n, K. Zhang, D. Bhandari, C. Boi*, R. Carbonell, X. Li, D. Chandra ...

topics (OpenAlex): Protein purification and stability; Monoclonal and Polyclonal Antibodies Research; Viral Infectious Diseases and Gene Expression in Insects
Source: ORCID
Added: September 16, 2025

2025 article

Graphene Oxide-Activated Cellular Ceramic Composite Monoliths for Protein Purification

Lalli, E., Onesti, R., Kovalevsky, A. V., Portugal, C. A. M., Crespo, J. G., Sarti, G. C., & Boi, C. (2025, September 8). Industrial & Engineering Chemistry Research.

co-author countries: Italy 🇮🇹 Portugal 🇵🇹
topics (OpenAlex): Graphene and Nanomaterials Applications; Graphene research and applications; Electrohydrodynamics and Fluid Dynamics
Source: ORCID
Added: September 16, 2025

2025 article

Flow-driven data intensification to accelerate autonomous inorganic materials discovery

Delgado-Licona, F., Alsaiari, A., Dickerson, H., Klem, P., Ghorai, A., Canty, R. B., … Abolhasani, M. (2025, July 14). Nature Chemical Engineering, Vol. 2, pp. 436–446.

By: F. Delgado-Licona n, A. Alsaiari n, H. Dickerson n, P. Klem n, A. Ghorai n, R. Canty n, J. Bennett n, P. Jha n ...

co-author countries: Mexico 🇲🇽
topics (OpenAlex): Innovative Microfluidic and Catalytic Techniques Innovation; Machine Learning in Materials Science; Catalytic Processes in Materials Science
Sources: Web Of Science, NC State University Libraries
Added: September 2, 2025

2025 article

P. pastoris (K. phaffi) perfusion processes: evaluation of cell retention techniques and integrated purification strategies

Linova, M., Kodiripaka, S., Martins, E., Pham, D., Sripada, S., Menegatti, S., … Woodley, J. (2025, March 1). New Biotechnology, Vol. 85, pp. 210–210.

By: M. Linova, S. Kodiripaka*, E. Martins*, D. Pham*, S. Sripada n, S. Menegatti n, R. Carbonell n, J. Woodley*

co-author countries: Denmark 🇩🇰
topics (OpenAlex): Molecular Biology Techniques and Applications; Viral Infectious Diseases and Gene Expression in Insects
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 4, 2025

2025 article

Continuous Tangential Flow Filtration to Isolate Extracellular Vesicles from Dairy By-Products

Onesti, R., Giancaterino, S., Masciolini, L., Piunti, C., Cimetta, E., & Boi, C. (2025, July 30). Industrial & Engineering Chemistry Research, Vol. 7.

co-author countries: Italy 🇮🇹
topics (OpenAlex): Extracellular vesicles in disease; Aerosol Filtration and Electrostatic Precipitation; Lattice Boltzmann Simulation Studies
Sources: ORCID, Web Of Science, NC State University Libraries
Added: August 1, 2025

2025 article

In vitro demonstration and in planta characterization of a condensed, reverse TCA (crTCA) cycle

Wilson, N., Smith-Moore, C., Xu, Y., Edwards, B., Hovary, C. L., Li, K., … Sederoff, H. (2025, June 9). Frontiers in Plant Science, Vol. 16.

By: N. Wilson n, C. Smith-Moore n, Y. Xu*, B. Edwards n, C. Hovary n, K. Li n, D. Aslett n, M. Ji n ...

author keywords: photosynthesis; CO2 fixation; synthetic biology; Camelina sativa; reverse TCA cycle; carbon capture
topics (OpenAlex): Plant tissue culture and regeneration; Plant Virus Research Studies; Plant Pathogens and Resistance
Sources: Web Of Science, NC State University Libraries
Added: June 30, 2025

2025 article

Dynamics of batch protein precipitation

Ehtesham, A., Sivaram, A., Siegel, S. D., Zanten, J., & Mansouri, S. S. (2025, May 29). Computers & Chemical Engineering, Vol. 200.

By: A. Ehtesham*, A. Sivaram*, S. Siegel n, J. Zanten n & S. Mansouri*

co-author countries: Denmark 🇩🇰
author keywords: Protein precipitation; Batch process; Population balance model; Machine learning
topics (OpenAlex): Protein purification and stability; Proteins in Food Systems; Microfluidic and Bio-sensing Technologies
Sources: Web Of Science, NC State University Libraries
Added: June 16, 2025

2025 journal article

Optimization of Bipolar Microsecond Electric Pulses for DNA Vaccine Delivery

IEEE Transactions on Biomedical Engineering, 1–12.

By: R. Williamson*, M. DeWitt, D. Elhanafi*, D. Zaharoff* & M. Sano*

author keywords: DNA; Electroporation; Vaccines; Protocols; Electric fields; Transfection; Three-dimensional displays; Solid modeling; Electrodes; Muscles; BJ fibroblast; DNA delivery; electroporation; HEK293; pulsed electric field; transfection
topics (OpenAlex): Microbial Inactivation Methods; Bacteriophages and microbial interactions; Toxin Mechanisms and Immunotoxins
Sources: ORCID, Crossref, NC State University Libraries, Web Of Science
Added: June 13, 2025

2025 article

Scalable membrane-based processes to isolate extracellular vesicles from lemon juice

Giancaterino, S., Onesti, R., & Boi, C. (2025, April 18). Separation and Purification Technology, Vol. 369.

co-author countries: Italy 🇮🇹
author keywords: Extracellular vesicles; Membrane processes; Scalable purification; Ultrafiltration/diafiltration; TFF; SEC; Ultracentrifugation
topics (OpenAlex): Extracellular vesicles in disease; MicroRNA in disease regulation; RNA regulation and disease
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: April 18, 2025

2025 journal article

Developing a process of lentivirus purification from recombinant fluids using peptide affinity ligands

Bioengineering & Translational Medicine, 10(6).

By: E. Barbieri n, G. Mollica n, S. Sripada n, S. Shastry n, Y. Wu n, A. Minzoni n, W. Smith n, E. Wuestenhagen* ...

co-author countries: Germany 🇩🇪
author keywords: affinity chromatography; lentivirus; membrane chromatography; peptide ligands; product polishing
topics (OpenAlex): Virus-based gene therapy research; CAR-T cell therapy research; Viral Infectious Diseases and Gene Expression in Insects
Sources: Web Of Science, NC State University Libraries, Crossref
Added: April 14, 2025

2025 article

Structural and kinetic characterization of an acetoacetyl-Coenzyme A: acetate Coenzyme A transferase from the extreme thermophile Thermosipho melanesiensis

Bing, R. G., Buhrman, G. K., Ford, K. C., Straub, C. T., Laemthong, T., Rose, R. B., … Kelly, R. M. (2025, January 27). Biochemical Journal, Vol. 482, pp. 225–240.

By: R. Bing n, G. Buhrman n, K. Ford n, C. Straub n, T. Laemthong n, R. Rose n, M. Adams, R. Kelly n

co-author countries: Canada 🇨🇦 Thailand 🇹🇭
MeSH headings : Kinetics; Acyl Coenzyme A / metabolism; Acyl Coenzyme A / chemistry; Coenzyme A-Transferases / chemistry; Coenzyme A-Transferases / metabolism; Coenzyme A-Transferases / genetics; Bacterial Proteins / chemistry; Bacterial Proteins / metabolism; Bacterial Proteins / genetics; Crystallography, X-Ray; Acetyl Coenzyme A / metabolism; Acetyl Coenzyme A / chemistry; Acetates / metabolism; Substrate Specificity; Recombinant Proteins / chemistry; Recombinant Proteins / metabolism; Recombinant Proteins / genetics; Firmicutes / enzymology
topics (OpenAlex): Microbial Metabolic Engineering and Bioproduction; Enzyme Structure and Function; Biochemical and Molecular Research
Sources: Web Of Science, NC State University Libraries
Added: April 14, 2025

2025 article

High-capacity nonwoven increases productivity of mAb purification in an all-membrane process

Fan, J., Lavoie, J., LeBarre, J., Menegatti, S., Pourdeyhimi, B., Boi, C., & Carbonell, R. G. (2025, March 19). Separation and Purification Technology, Vol. 364.

co-author countries: Italy 🇮🇹
author keywords: Multimodal anion exchange; Membrane adsorber; Membrane chromatography; Nonwoven membrane; monoclonal antibodies (mAbs)
topics (OpenAlex): Recycling and Waste Management Techniques; Extraction and Separation Processes; Protein purification and stability
Sources: ORCID, Web Of Science, NC State University Libraries
Added: March 19, 2025

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