Golden Leaf Biomanufacturing Training and Education Center

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2025 journal article

NANOFILTRATION EFFICIENCY IN THE PURIFICATION OF LACTOSE FROM ULTRAFILTERED ACID WHEY

Journal of Food Engineering.

By: R. Onesti, M. Roselli, C. Boi*  & S. Bandini

topics (OpenAlex): Membrane Separation Technologies; Electrohydrodynamics and Fluid Dynamics; Electrospun Nanofibers in Biomedical Applications
Source: ORCID
Added: November 1, 2025

2025 journal article

Flow-through polishing of Protein A eluates: Removal of high and low molecular weight impurities by size-exclusion-mixed-mode chromatography

Journal of Chromatography A.

By: J. LeBarre  n, W. Chu  n, K. Zhang  n, D. Bhandari, C. Boi n , R. Carbonell  n, X. Li *, D. Chandra * ...

topics (OpenAlex): Protein purification and stability; Monoclonal and Polyclonal Antibodies Research; Viral Infectious Diseases and Gene Expression in Insects
Source: ORCID
Added: September 16, 2025

2025 journal article

Graphene Oxide-Activated Cellular Ceramic Composite Monoliths for Protein Purification

Industrial & Engineering Chemistry Research.

By: E. Lalli*, R. Onesti *, A. Kovalevsky *, C. Portugal *, J. Crespo *, G. Sarti *, C. Boi n 

co-author countries: Italy 🇮🇹 Portugal 🇵🇹
topics (OpenAlex): Graphene and Nanomaterials Applications; Graphene research and applications; Electrohydrodynamics and Fluid Dynamics
Source: ORCID
Added: September 16, 2025

2025 journal article

Flow-driven data intensification to accelerate autonomous inorganic materials discovery

NATURE CHEMICAL ENGINEERING, 2(7), 436–446.

By: F. Delgado-Licona, A. Alsaiari, H. Dickerson*, P. Klem, A. Ghorai*, R. Canty, J. Bennett*, P. Jha* ...

topics (OpenAlex): Innovative Microfluidic and Catalytic Techniques Innovation; Machine Learning in Materials Science; Catalytic Processes in Materials Science
Sources: Web Of Science, NC State University Libraries
Added: September 2, 2025

2025 article

<i>P.</i><i> pastoris</i> (K.<i> phaffi)</i> perfusion processes: evaluation of cell retention techniques and integrated purification strategies

Linova, M., Kodiripaka, S., Martins, E., Pham, D., Sripada, S., Menegatti, S., … Woodley, J. (2025, March 25). NEW BIOTECHNOLOGY, Vol. 85, pp. 210–210.

By: M. Linova, S. Kodiripaka, E. Martins, D. Pham, S. Sripada, S. Menegatti* , R. Carbonell*, J. Woodley

topics (OpenAlex): Molecular Biology Techniques and Applications; Viral Infectious Diseases and Gene Expression in Insects
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 4, 2025

2025 article

Continuous Tangential Flow Filtration to Isolate Extracellular Vesicles from Dairy By-Products

Onesti, R., Giancaterino, S., Masciolini, L., Piunti, C., Cimetta, E., & Boi, C. (2025, July 30). INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, Vol. 7.

By: R. Onesti *, S. Giancaterino *, L. Masciolini n, C. Piunti *, E. Cimetta * & C. Boi n 

co-author countries: Italy 🇮🇹
topics (OpenAlex): Extracellular vesicles in disease; Aerosol Filtration and Electrostatic Precipitation; Lattice Boltzmann Simulation Studies
Sources: ORCID, Web Of Science, NC State University Libraries
Added: August 1, 2025

2025 journal article

<i>In vitro</i> demonstration and <i>in planta</i> characterization of a condensed, reverse TCA (crTCA) cycle

FRONTIERS IN PLANT SCIENCE, 16.

By: N. Wilson  n, C. Smith-Moore, Y. Xu *, B. Edwards n, C. La Hovary n, K. Li  n, D. Aslett n, M. Ji n ...

author keywords: photosynthesis; CO2 fixation; synthetic biology; Camelina sativa; reverse TCA cycle; carbon capture
topics (OpenAlex): Plant tissue culture and regeneration; Plant Virus Research Studies; Plant Pathogens and Resistance
Sources: Web Of Science, NC State University Libraries
Added: June 30, 2025

2025 journal article

Dynamics of batch protein precipitation

COMPUTERS & CHEMICAL ENGINEERING, 200.

By: A. Ehtesham, A. Sivaram, S. Siegel*, J. Zanten*  & S. Mansouri

author keywords: Protein precipitation; Batch process; Population balance model; Machine learning
topics (OpenAlex): Protein purification and stability; Proteins in Food Systems; Microfluidic and Bio-sensing Technologies
Sources: Web Of Science, NC State University Libraries
Added: June 16, 2025

2025 journal article

Optimization of Bipolar Microsecond Electric Pulses for DNA Vaccine Delivery

IEEE Transactions on Biomedical Engineering, 72(8), 1–12.

By: R. Williamson*, M. DeWitt, D. Elhanafi*, D. Zaharoff* & M. Sano

author keywords: DNA; Electroporation; Vaccines; Protocols; Electric fields; Transfection; Three-dimensional displays; Solid modeling; Electrodes; Muscles; BJ fibroblast; DNA delivery; electroporation; HEK293; pulsed electric field; transfection
topics (OpenAlex): Microbial Inactivation Methods; Bacteriophages and microbial interactions; Toxin Mechanisms and Immunotoxins
Sources: ORCID, Crossref, NC State University Libraries, Web Of Science
Added: June 13, 2025

2025 journal article

Scalable membrane-based processes to isolate extracellular vesicles from lemon juice

SEPARATION AND PURIFICATION TECHNOLOGY, 369.

By: S. Giancaterino, R. Onesti & C. Boi* 

author keywords: Extracellular vesicles; Membrane processes; Scalable purification; Ultrafiltration/diafiltration; TFF; SEC; Ultracentrifugation
topics (OpenAlex): Extracellular vesicles in disease; MicroRNA in disease regulation; RNA regulation and disease
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: April 18, 2025

2025 article

Developing a process of lentivirus purification from recombinant fluids using peptide affinity ligands

Barbieri, E., Mollica, G. N., Sripada, S. A., Shastry, S., Wu, Y., Minzoni, A., … Menegatti, S. (2025, April 7). BIOENGINEERING & TRANSLATIONAL MEDICINE, Vol. 4.

By: E. Barbieri n, G. Mollica n, S. Sripada n, S. Shastry n, Y. Wu n, A. Minzoni n, W. Smith n, E. Wuestenhagen* ...

co-author countries: Germany 🇩🇪
author keywords: affinity chromatography; lentivirus; membrane chromatography; peptide ligands; product polishing
topics (OpenAlex): Virus-based gene therapy research; CAR-T cell therapy research; Viral Infectious Diseases and Gene Expression in Insects
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: April 14, 2025

2025 journal article

Structural and kinetic characterization of an acetoacetyl-Coenzyme A: acetate Coenzyme A transferase from the extreme thermophile Thermosipho melanesiensis

BIOCHEMICAL JOURNAL, 482(4), 225–240.

By: R. Bing n , G. Buhrman n, K. Ford n, C. Straub n, T. Laemthong n, R. Rose  n, M. Adams, R. Kelly n 

MeSH headings : Kinetics; Acyl Coenzyme A / metabolism; Acyl Coenzyme A / chemistry; Coenzyme A-Transferases / chemistry; Coenzyme A-Transferases / metabolism; Coenzyme A-Transferases / genetics; Bacterial Proteins / chemistry; Bacterial Proteins / metabolism; Bacterial Proteins / genetics; Crystallography, X-Ray; Acetyl Coenzyme A / metabolism; Acetyl Coenzyme A / chemistry; Acetates / metabolism; Substrate Specificity; Recombinant Proteins / chemistry; Recombinant Proteins / metabolism; Recombinant Proteins / genetics; Firmicutes / enzymology
topics (OpenAlex): Microbial Metabolic Engineering and Bioproduction; Enzyme Structure and Function; Biochemical and Molecular Research
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: April 14, 2025

2025 journal article

High-capacity nonwoven increases productivity of mAb purification in an all-membrane process

SEPARATION AND PURIFICATION TECHNOLOGY, 364.

By: J. Fan*, J. Lavoie*, J. LeBarre*, S. Menegatti* , B. Pourdeyhimi* , C. Boi* , R. Carbonell*

author keywords: Multimodal anion exchange; Membrane adsorber; Membrane chromatography; Nonwoven membrane; monoclonal antibodies (mAbs)
topics (OpenAlex): Recycling and Waste Management Techniques; Extraction and Separation Processes; Protein purification and stability
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: ORCID, Web Of Science, NC State University Libraries
Added: March 19, 2025

2025 journal article

CO2 Monitoring in Non-Invasive Ventilation (NIV) Helmets: A Bench Study Assessment of Sensor Integration

Heliyon.

By: C. Gironi, R. Amiri, M. Baschetti, C. Boi* , . cercenelli, B. Bortolani, E. Marcelli

topics (OpenAlex): Respiratory Support and Mechanisms; Cardiac Arrest and Resuscitation; Chronic Obstructive Pulmonary Disease (COPD) Research
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Source: ORCID
Added: March 5, 2025

2025 journal article

Microfluidic loading of verteporfin into extracellular vesicles for neuroblastoma therapy

Lab on a Chip.

By: C. Piunti *, S. Micheli *, S. Giancaterino*, P. Fusco *, C. Boi*  & E. Cimetta *

co-author countries: Italy 🇮🇹
MeSH headings : Verteporfin / pharmacology; Extracellular Vesicles / metabolism; Extracellular Vesicles / chemistry; Neuroblastoma / metabolism; Neuroblastoma / drug therapy; Neuroblastoma / pathology; Humans; Cell Line, Tumor; Lab-On-A-Chip Devices; Microfluidic Analytical Techniques / instrumentation; Mesenchymal Stem Cells / metabolism; Mesenchymal Stem Cells / cytology; Mesenchymal Stem Cells / drug effects; Cell Survival / drug effects
topics (OpenAlex): Extracellular vesicles in disease; Nanopore and Nanochannel Transport Studies; Nanoplatforms for cancer theranostics
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: March 5, 2025

2024 journal article

Sorbent-based dialysate regeneration for the wearable artificial kidney: Advancing material innovation via experimental and computational studies

Separation and Purification Technology.

By: T. Fabiani, M. Zarghamidehaghani, C. Boi* , S. Dimartino, S. Kentish & M. Angelis

topics (OpenAlex): Electrospun Nanofibers in Biomedical Applications; Supercapacitor Materials and Fabrication; Tissue Engineering and Regenerative Medicine
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
9. Industry, Innovation and Infrastructure (OpenAlex)
Source: ORCID
Added: March 5, 2025

2024 article

Draft genome sequences of <i>Listeria monocytogenes</i> strains from human listeriosis in Sweden harboring premature stop codons in the virulence determinant <i>inlA</i>

Lee, S., Brown, P., Tham, W., Danielsson-Tham, M.-L., Lopez-Valladares, G., Ward, T., … Kathariou, S. (2024, October 21). (D. Rasko, Ed.). MICROBIOLOGY RESOURCE ANNOUNCEMENTS, Vol. 10.

By: S. Lee *, P. Brown n , W. Tham *, M. Danielsson-Tham*, G. Lopez-Valladares*, T. Ward *, D. Elhanafi n, Y. Chen *, S. Kathariou n

co-author countries: Korea (Republic of) 🇰🇷 Sweden 🇸🇪

Ed(s): D. Rasko

author keywords: Listeria monocytogenes; inlA; premature stop codon; clinical; Sweden; internalin A
topics (OpenAlex): Listeria monocytogenes in Food Safety; Salmonella and Campylobacter epidemiology; Essential Oils and Antimicrobial Activity
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: October 21, 2024

2024 journal article

Nanostructured Affinity Membrane to Isolate Extracellular Vesicles from Body Fluids for Diagnostics and Regenerative Medicine

Membranes.

By: M. Torsello *, M. Animini*, C. Gualandi *, F. Perut *, A. Pollicino *, C. Boi* , M. Focarete *

co-author countries: Italy 🇮🇹
topics (OpenAlex): Extracellular vesicles in disease; Electrospun Nanofibers in Biomedical Applications; RNA Interference and Gene Delivery
Source: ORCID
Added: October 1, 2024

2024 journal article

Serotype-agnostic affinity purification of adeno-associated virus (AAV) via peptide-functionalized chromatographic resins

JOURNAL OF CHROMATOGRAPHY A, 1734.

By: S. Shastry n, E. Barbieri n, A. Minzoni n, W. Chu n, S. Johnson n, M. Stoops n, J. Pancorbo n, G. Gilleskie n  ...

author keywords: Gene therapy; Adeno-associated virus; Peptide ligands; Affinity chromatography; Transduction activity
MeSH headings : Dependovirus / isolation & purification; Dependovirus / genetics; Dependovirus / chemistry; Chromatography, Affinity / methods; Peptides / chemistry; Peptides / isolation & purification; Serogroup; Humans; Hydrogen-Ion Concentration; Genetic Vectors; HEK293 Cells
topics (OpenAlex): Virus-based gene therapy research; RNA Interference and Gene Delivery; Viral Infectious Diseases and Gene Expression in Insects
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: ORCID, Web Of Science, NC State University Libraries
Added: September 1, 2024

2024 chapter book

Ion-exchange membrane chromatography

By: R. Onesti*, S. Giancaterino*, M. Roselli *, S. Bandini* & C. Boi* 

co-author countries: Italy 🇮🇹
topics (OpenAlex): Protein purification and stability; Analytical Chemistry and Chromatography; Microfluidic and Capillary Electrophoresis Applications
Sources: Crossref, NC State University Libraries
Added: August 23, 2024

2024 journal article

Recovery of lactose from acid whey by nanofiltration: An experimental study

Separation and Purification Technology, 353, 128303.

By: M. Roselli *, R. Onesti*, C. Boi*  & S. Bandini*

co-author countries: Italy 🇮🇹
topics (OpenAlex): Membrane Separation Technologies; Membrane-based Ion Separation Techniques; Electrohydrodynamics and Fluid Dynamics
Sources: Crossref, NC State University Libraries
Added: August 23, 2024

2024 journal article

Negative Voltage Electrospinning for the Production of Highly Efficient PVDF Filters

Macromolecular Materials and Engineering.

By: C. Gotti *, M. Torsello *, R. Onesti*, G. Tanganelli*, A. Sensini *, C. Boi* , D. Fabiani *, M. Focarete *, A. Zucchelli *

co-author countries: Italy 🇮🇹 Netherlands 🇳🇱
topics (OpenAlex): Advanced Sensor and Energy Harvesting Materials; Electrospun Nanofibers in Biomedical Applications; Conducting polymers and applications
Source: ORCID
Added: August 22, 2024

2024 article

Peptide ligands for the universal purification of exosomes by affinity chromatography

Kilgore, R. E., Moore, B. D., Sripada, S. A., Chu, W., Shastry, S., Barbieri, E., … Menegatti, S. (2024, August 4). BIOTECHNOLOGY AND BIOENGINEERING, Vol. 8.

By: R. Kilgore n, B. Moore n, S. Sripada n, W. Chu n, S. Shastry n, E. Barbieri n, S. Hu n , W. Tian * ...

co-author countries: Denmark 🇩🇰
author keywords: affinity chromatography; bioseparations; exosomes; gene delivery; peptide ligands
MeSH headings : Exosomes / chemistry; Exosomes / metabolism; Chromatography, Affinity / methods; Ligands; Humans; Peptides / chemistry; Peptides / metabolism; Peptides / isolation & purification
topics (OpenAlex): Extracellular vesicles in disease; RNA Interference and Gene Delivery; Inhalation and Respiratory Drug Delivery; Lipid Membrane Structure and Behavior
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 14, 2024

2024 journal article

Editorial for the Special Issue "Preparation and Application of Advanced Functional Membranes"

MEMBRANES, 14(5).

By: A. Gugliuzza * & C. Boi n 

co-author countries: Italy 🇮🇹
topics (OpenAlex): Membrane Separation and Gas Transport; Membrane Separation Technologies; Fuel Cells and Related Materials
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: June 11, 2024

2024 article

Multiangle Light Scattering as a Lentivirus Purification Process Analytical Technology

Sripada, S. A., Barbieri, E., Shastry, S., Wuestenhagen, E., Aldinger, A., Rammo, O., … Menegatti, S. (2024, May 28). ANALYTICAL CHEMISTRY, Vol. 5.

By: S. Sripada n, E. Barbieri n, S. Shastry n, E. Wuestenhagen*, A. Aldinger*, O. Rammo*, M. Schulte*, M. Daniele n , S. Menegatti n 

co-author countries: Germany 🇩🇪
MeSH headings : Lentivirus / genetics; Lentivirus / isolation & purification; Humans; Dynamic Light Scattering; Chromatography, Gel; HEK293 Cells; Particle Size; Genetic Vectors / genetics; Genetic Vectors / isolation & purification
topics (OpenAlex): Spectroscopy Techniques in Biomedical and Chemical Research; Viral Infectious Diseases and Gene Expression in Insects; Protein purification and stability
UN Sustainable Development Goals Color Wheel
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: June 11, 2024

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