Golden Leaf Biomanufacturing Training and Education Center

Works Published in 2011

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Displaying all 11 works

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2011 journal article

Effects of peptide density and elution pH on affinity chromatographic purification of human immunoglobulins A and M

JOURNAL OF CHROMATOGRAPHY A, 1218(46), 8344–8352.

author keywords: Human immunoglobulin; IgG; IgA; IgM; Affinity chromatography; Binding site
MeSH headings : Amino Acid Sequence; Binding Sites; Chromatography, Affinity / instrumentation; Chromatography, Affinity / methods; Electrophoresis, Polyacrylamide Gel; Humans; Hydrogen-Ion Concentration; Immunoglobulin A / chemistry; Immunoglobulin A / isolation & purification; Immunoglobulin M / chemistry; Immunoglobulin M / isolation & purification; Immunosorbent Techniques; Molecular Sequence Data; Oligopeptides / chemistry; Protein Conformation; Sequence Alignment
TL;DR: Although HYFKFD and HFRRHL exhibited their ability for isolation of human IgG, IgA and IgM from cMEM as well, HWRGWV is the best option among them for large-scale purification (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Selectively solvated triblock copolymer networks under biaxial strain

APPLIED PHYSICS LETTERS, 99(10).

By: A. Krishnan n, J. Zanten n, S. Seifert*, B. Lee* & R. Spontak n

UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Effects of UV Exposure and Initiator Concentration on the Spatial Variation of Poly(glycidyl methacrylate) Grafts on Nonwoven Fabrics

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 50(10), 6115–6123.

By: Y. Zheng, P. Gurgel* & R. Carbonell n

UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Polystyrene/Decahydronaphthalene/Propane Phase Equilibria and Polymer Conformation Properties from Intrinsic Viscosities

JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS, 49(15), 1093–1100.

By: N. Cain n, A. Haywood n, G. Roberts n, D. Kiserow n & R. Carbonell n

author keywords: conformational analysis; decahydronaphthalene; intrinsic viscosity; miscibility; phase behavior; phase diagrams; phase separation; polystyrene; viscosity
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Performance of hexamer peptide ligands for affinity purification of immunoglobulin G from commercial cell culture media

Journal of Chromatography A, 1218(13), 1691–1700.

author keywords: Hexapeptide ligand; IgG purification; Monoclonal antibody; Affinity chromatography; Regeneration
MeSH headings : Adsorption; Animals; Antibodies, Monoclonal / chemistry; Antibodies, Monoclonal / isolation & purification; CHO Cells; Caprylates; Cell Culture Techniques; Chromatography, Affinity / methods; Cricetinae; Cricetulus; Culture Media; Electrophoresis, Polyacrylamide Gel; Humans; Immunoglobulin G / chemistry; Immunoglobulin G / isolation & purification; Oligopeptides / chemistry; Phosphoric Acids; Protein Binding; Sodium Chloride; Urea
TL;DR: Issues related to the successful application of the hexapeptide ligands, on a commercial methacrylate chromatographic resin, for the purification of IgG from mammalian cell culture fluids are addressed. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Comparative Analysis of the 15.5kD Box C/D snoRNP Core Protein in the Primitive Eukaryote Giardia lamblia Reveals Unique Structural and Functional Features

BIOCHEMISTRY, 50(14), 2907–2918.

MeSH headings : Amino Acid Sequence; Animals; Binding Sites / genetics; Circular Dichroism; Crystallography, X-Ray; Giardia lamblia / metabolism; Humans; Methyltransferases / chemistry; Methyltransferases / metabolism; Models, Molecular; Molecular Sequence Data; Molecular Weight; Phylogeny; Protein Binding; Protein Stability; Protein Structure, Secondary; Protein Structure, Tertiary; Protozoan Proteins / chemistry; Protozoan Proteins / genetics; Protozoan Proteins / metabolism; RNA / genetics; RNA / metabolism; Ribonucleoproteins, Small Nucleolar / chemistry; Ribonucleoproteins, Small Nucleolar / classification; Ribonucleoproteins, Small Nucleolar / metabolism; Sequence Homology, Amino Acid; Temperature
TL;DR: Comp comparative sequence analysis indicated a distinct evolutionary position between Archaea and Eukarya, and the Giardia 15.5kD protein occupies a unique position in the evolution of this box C/D RNP core protein retaining structural and functional features characteristic of both archaeal L7Ae and higher eukaryotic 15. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Microviscoelasticity of soft repulsive sphere dispersions: Tracer particle microrheology of triblock copolymer micellar liquids and soft crystals

JOURNAL OF CHEMICAL PHYSICS, 134(17).

By: S. Tanner n, S. Amin n, C. Kloxin n & J. Zanten n

TL;DR: The observation of subdiffusive colloidal sphere thermal motion at short time-scales is strong evidence that the observed microscopic viscoelastic properties reflect the dynamics of individual micelles rather than a dispersion of micellar crystallites. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Modeling the thermodynamic and transport properties of decahydronaphthalene/propane mixtures: Phase equilibria, density, and viscosity

FLUID PHASE EQUILIBRIA, 305(1), 25–33.

By: N. Cain n, G. Roberts n, D. Kiserow n & R. Carbonell n

author keywords: Phase equilibria; Modified Sanchez-Lacombe Equation of State; Decahydronaphthalene; Propane; density; Viscosity; Volume expansion; Viscosity reduction; Free volume model for viscosity
UN Sustainable Development Goal Categories
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Functional Analysis of a Novel Motif Conserved across Geminivirus Rep Proteins

JOURNAL OF VIROLOGY, 85(3), 1182–1192.

By: T. Nash n, M. Dallas n, M. Reyes n, G. Buhrman n, J. Ascencio-Ibanez n & L. Hanley-Bowdoin n

MeSH headings : Amino Acid Motifs; Amino Acid Sequence; Conserved Sequence; DNA Helicases / genetics; DNA Helicases / metabolism; DNA, Viral / metabolism; Geminiviridae / enzymology; Geminiviridae / genetics; Geminiviridae / physiology; Molecular Sequence Data; Mutation, Missense; Plant Diseases / virology; Protein Binding; Sequence Alignment; Tobacco / virology; Trans-Activators / genetics; Trans-Activators / metabolism; Viral Proteins / genetics; Viral Proteins / metabolism; Virulence; Virus Replication
TL;DR: It is established that the GRS is a conserved, essential motif characteristic of an ancient lineage of rolling-circle initiators and support the idea that geminiviruses may have evolved from plasmids associated with phytoplasma or algae. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Allosteric Modulation of Ras-GTP Is Linked to Signal Transduction through RAF Kinase

JOURNAL OF BIOLOGICAL CHEMISTRY, 286(5), 3323–3331.

By: G. Buhrman*, V. Kumar, M. Cirit n, J. Haugh n & C. Mattos*

MeSH headings : Allosteric Regulation; Allosteric Site; Animals; Guanosine Triphosphate / metabolism; Kinetics; Mice; Mutation, Missense; NIH 3T3 Cells; Protein Conformation; Protein Stability; Signal Transduction; raf Kinases / metabolism; ras Proteins / genetics; ras Proteins / metabolism
TL;DR: The allosteric switch is linked to the control of signaling in the Ras/Raf/MEK/ERK pathway, supporting a GTPase-activating protein-independent model for duration of the Ras-Raf complex. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Transport and Binding Characterization of a Novel Hybrid Particle Impregnated Membrane Material for Bioseparations

BIOTECHNOLOGY PROGRESS, 27(1), 129–139.

By: M. Herigstad n, P. Gurgel & R. Carbonell n

author keywords: particle-impregnated membrane; process integration; mathematical modeling
MeSH headings : Adsorption; Chromatography / instrumentation; Membranes, Artificial; Microscopy, Electron, Scanning; Models, Theoretical; Thermodynamics
TL;DR: The results suggest that PIM constructs can be designed to process viscous mobile phases containing particulates while retaining the desirable binding characteristics of the embedded chromatographic resin and could find uses in adsorption separation processes from complex feed streams such as whole blood, cell culture, and food processing. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

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