Works Published in 2012
Displaying all 9 works
Sorted by most recent date added to the index first, which may not be the same as publication date order.
2012 journal article
Purification of polyclonal antibodies from Cohn fraction II + III, skim milk, and whey by affinity chromatography using a hexamer peptide ligand
Journal of Separation Science, 35(22), 3139–3148.
author keywords: Affinity chromatography; Immunoglobulin G; Milk; Peptide ligands; Plasma
MeSH headings : Animals; Antibodies / chemistry; Antibodies / isolation & purification; Blood Proteins / chemistry; Blood Proteins / isolation & purification; Cattle; Chromatography, Affinity / instrumentation; Chromatography, Affinity / methods; Humans; Ligands; Milk / chemistry; Milk Proteins / chemistry; Milk Proteins / isolation & purification; Peptides / chemistry; Whey Proteins
TL;DR:
HWRGWV, a peptide that binds specifically to the Fc fragment of human immunoglobulin G (IgG), was used for the purification of IgG from Cohn fraction II + III of human plasma and from bovine skim milk and whey.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Source: Crossref
Added: August 28, 2020
2012 journal article
Bulky Adamantanethiolate and Cyclohexanethiolate Ligands Favor Smaller Gold Nanoparticles with Altered Discrete Sizes
ACS NANO, 6(6), 4903–4911.
author keywords: steric effects; bulky ligands; magic sizes; discrete sizes; clusters; gold; thiol
MeSH headings : Adamantane; Crystallization / methods; Cyclohexanes / chemistry; Gold / chemistry; Ligands; Macromolecular Substances / chemistry; Materials Testing; Molecular Conformation; Nanoparticles / chemistry; Nanoparticles / ultrastructure; Particle Size; Sulfhydryl Compounds / chemistry; Surface Properties
TL;DR:
Use of bulky ligands in the synthesis of metal nanoparticles (NPs) gives smaller core sizes, sharpens the size distribution, and alters the discrete sizes, which may also lead to the discovery of new discrete sizes in other systems.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Design of pH Sensitive Binding Proteins from the Hyperthermophilic Sso7d Scaffold
PLOS ONE, 7(11).
MeSH headings : Carrier Proteins / genetics; Carrier Proteins / metabolism; Epitopes / genetics; Escherichia coli / genetics; Escherichia coli / metabolism; Histidine / genetics; Histidine / metabolism; Humans; Hydrogen-Ion Concentration; Immunoglobulin Fc Fragments / genetics; Immunoglobulin Fc Fragments / metabolism; Immunoglobulin G / genetics; Immunoglobulin G / metabolism; Mutagenesis; Sulfolobus solfataricus / genetics; Sulfolobus solfataricus / metabolism
TL;DR:
P pH sensitive hFc binders are attractive candidates for use in chromatography, to achieve elution of IgG under milder pH conditions, but further studies are needed to evaluate if the Sso7d mutants identified in this study are indeed useful as affinity ligands in Chromatography.
(via Semantic Scholar)
journals.plos.org (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Shift in the Equilibrium between On and Off States of the Allosteric Switch in Ras-GppNHp Affected by Small Molecules and Bulk Solvent Composition
BIOCHEMISTRY, 51(31), 6114–6126.
MeSH headings : Acetates / metabolism; Acetates / pharmacology; Allosteric Regulation / drug effects; Allosteric Site / drug effects; Calcium Compounds / metabolism; Calcium Compounds / pharmacology; Catalytic Domain; Dithioerythritol / metabolism; Dithioerythritol / pharmacology; Dithiothreitol / metabolism; Dithiothreitol / pharmacology; Enzyme Stability / drug effects; Guanosine Triphosphate / metabolism; Guanylyl Imidodiphosphate / chemistry; Hydrolysis; Isoenzymes / chemistry; Isoenzymes / metabolism; Models, Molecular; Polyethylene Glycols / pharmacology; Protein Structure, Secondary; Small Molecule Libraries / metabolism; Small Molecule Libraries / pharmacology; Solutions; Solvents / chemistry; Solvents / pharmacology; ras Proteins / chemistry; ras Proteins / metabolism
TL;DR:
It is shown with high-resolution structures that calcium acetate and either dithioerythritol (DTE) or dithiothreitol (DTT) soaked into H-Ras-GppNHp crystals in the presence of a moderate amount of poly(ethylene glycol) (PEG) can selectively shift the equilibrium to the "on" state, where the active site appears to be poised for catalysis (calcium acetate), or to what
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
8. Decent Work and Economic Growth
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Purification of human immunoglobulins A, G and M from Cohn fraction II/III by small peptide affinity chromatography
JOURNAL OF CHROMATOGRAPHY A, 1262, 169–179.
author keywords: Hexamer peptide; IVIG; Cohn fraction; Elution additive; PEG; Ammonium sulfate
MeSH headings : Ammonium Sulfate / chemistry; Arginine / chemistry; Blood Proteins / chemistry; Caprylates / chemistry; Chromatography, Affinity / methods; Ethylene Glycols / chemistry; Humans; Immunoglobulin Isotypes / blood; Immunoglobulin Isotypes / isolation & purification; Peptides / chemistry; Salts / chemistry
TL;DR:
Attempts to purify human IgG, IgA and IgM from Cohn fraction II/III using HWRGWV affinity peptide resin using pretreatment methods comparable to current chromatographic purification methods involving two chromatography steps when hIgG is isolated from plasma fractions are described.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Process for purification of monoclonal antibody expressed in transgenic Lemna plant extract using dextran-coated charcoal and hexamer peptide affinity resin
Journal of Chromatography A, 1260, 61–66.
author keywords: Dextran-coated charcoal; Transgenic plants; Hexapeptide ligand; IgG purification; Monoclonal antibody; Phenolics; Lemna minor
MeSH headings : Antibodies, Monoclonal / isolation & purification; Antibodies, Monoclonal / metabolism; Araceae / chemistry; Araceae / metabolism; Charcoal / chemistry; Chromatography, Affinity / methods; Dextrans / chemistry; Humans; Immunoglobulin G / isolation & purification; Immunoglobulin G / metabolism; Peptides / chemistry; Peptides / metabolism; Plant Extracts / chemistry; Plants, Genetically Modified / chemistry; Plants, Genetically Modified / metabolism; Protein Binding; Staphylococcal Protein A / chemistry; Staphylococcal Protein A / isolation & purification
TL;DR:
The development of a pretreatment and affinity separation process for the purification of a monoclonal antibody from transgenic Lemna plant extract and the performance of the hexamer peptide resin after the pretreatment step was found to be similar to that obtained with a commercial Protein A resin.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Coselection of Cadmium and Benzalkonium Chloride Resistance in Conjugative Transfers from Nonpathogenic Listeria spp. to Other Listeriae
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 78(21), 7549–7556.
MeSH headings : Anti-Infective Agents, Local / pharmacology; Bacterial Proteins / genetics; Benzalkonium Compounds / pharmacology; Cadmium / pharmacology; Conjugation, Genetic; Drug Resistance, Bacterial / genetics; Food Handling; Gene Transfer, Horizontal; Listeria / drug effects; Listeria / genetics; Listeria / pathogenicity; Temperature
TL;DR:
The results suggest that nonpathogenic Listeria spp.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Porcine parvovirus removal using trimer and biased hexamer peptides
BIOTECHNOLOGY JOURNAL, 7(4), 558–565.
author keywords: Affinity adsorption; Biosafety; Bioseparation; Ligand; Virus removal
MeSH headings : Adsorption; Chromatography, Affinity; Humans; Parvovirus, Porcine / chemistry; Parvovirus, Porcine / isolation & purification; Peptides / chemical synthesis; Peptides / chemistry; Plasma / chemistry; Plasma / virology
TL;DR:
This study demonstrates that trimeric and hexameric peptides may have different, matrix‐specific roles to play in virus removal applications, and in general, the hexamer ligand may perform better for binding of specific viruses, whereas the trimer ligand might have more broadly reactive virus‐binding properties.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2012 journal article
Alkaline-stable peptide ligand affinity adsorbents for the purification of biomolecules
Journal of Chromatography A, 1245, 55–64.
author keywords: Peptide ligand; Affinity adsorbent; NaOH stability; Modification of surface chemistry; Solid-phase peptide synthesis; Cleaning-in-place
MeSH headings : Adsorption; Alkalies / chemistry; Chromatography, Affinity / instrumentation; Chromatography, Affinity / methods; Immunoglobulin G / chemistry; Immunoglobulin G / isolation & purification; Ligands; Peptides / chemistry; Protein Binding
TL;DR:
It was found that the ligand leaching was caused by the coupling of a significant amount of peptide by alkaline-labile ester bonds, and the method presented has a broad application since it is valid for any peptide ligand identified for the purification of a biopharmaceutical target.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
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