Golden Leaf Biomanufacturing Training and Education Center

Works Published in 2019

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Displaying all 7 works

Sorted by most recent date added to the index first, which may not be the same as publication date order.

2019 article

Valorization of Eggshell Waste into Supported Copper Catalysts for Partial Oxidation of Methane

INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH.

By: G. Karoshi n, P. Kolar n, S. Shah n & G. Gilleskie n

author keywords: Eggshell; Copper; Catalyst; Methane; Partial oxidation
UN Sustainable Development Goal Categories
13. Climate Action (Web of Science)
Source: ORCID
Added: January 26, 2020

2019 journal article

Multiplexed Competitive Screening of One-Bead-One-Component Combinatorial Libraries Using a ClonePix 2 Colony Sorter

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 20(20).

By: R. Lavoie n, A. Fazio n, R. Carbonell n & S. Menegatti n

author keywords: library screening; bioactive peptides; ligand development; affinity chromatography; affinity ligand; ClonePix 2
MeSH headings : Animals; Cell Line; Chromatography, Affinity / methods; Drug Discovery / methods; Humans; Immunoglobulin G; Ligands; Optical Imaging / methods; Peptide Library; Small Molecule Libraries
TL;DR: This work presents a method for multiplexed solid-phase peptide library screening using a ClonePix 2 Colony Picker that integrates orthogonal fluorescent labeling for positive selection against a target protein and negative selection against competitor species with semi-quantitative tracking of target vs. competitor binding for every library bead. (via Semantic Scholar)
Source: Web Of Science
Added: December 16, 2019

2019 journal article

A Microalgae Biocomposite-Integrated Spinning Disk Bioreactor (SDBR): Toward a Scalable Engineering Approach for Bioprocess Intensification in Light-Driven CO2 Absorption Applications

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 58(15), 5936–5949.

TL;DR: Overall, the increased CO2 biofixation with a greatly reduced biocomposite surface area and the high cell retention in this proof-of-concept technology highlight the bioprocess intensificatio... (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Source: Web Of Science
Added: May 13, 2019

2019 journal article

Silica resins and peptide ligands to develop disposable affinity adsorbents for antibody purification

BIOCHEMICAL ENGINEERING JOURNAL, 145, 53–61.

author keywords: Silica resins; Peptide ligands; Monoclonal antibodies; Llama antibodies; Rabbit antibodies
TL;DR: It is demonstrated that porous silica, a non-traditional substrate for protein purification, shows great promise as potentially single-use affinity adsorbent forprotein purification. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: May 13, 2019

2019 journal article

Targeted Capture of Chinese Hamster Ovary Host Cell Proteins: Peptide Ligand Discovery

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 20(7).

By: R. Lavoie n, A. Fazio n, R. Blackburn n, M. Goshe n, R. Carbonell n & S. Menegatti n

author keywords: therapeutic antibodies; host cell proteins; protein purification; peptide ligands
MeSH headings : Animals; CHO Cells; Chromatography, Affinity; Chromatography, Liquid; Cricetinae; Cricetulus; Humans; Peptides / chemistry; Peptides / isolation & purification; Proteomics / methods
TL;DR: Development of synthetic peptide ligands capable of capturing a broad spectrum of Chinese hamster ovary (CHO) HCPs with a combination of peptide species that allow for advanced mixed-mode binding is described. (via Semantic Scholar)
Source: Web Of Science
Added: May 6, 2019

2019 journal article

Use of a Branched Linker for Enhanced Biosensing Properties in IgG Detection from Mixed Chinese Hamster Ovary Cell Cultures

BIOCONJUGATE CHEMISTRY, 30(3), 815–825.

MeSH headings : Adsorption; Amino Acid Sequence; Animals; Biosensing Techniques; CHO Cells; Complex Mixtures; Cricetinae; Cricetulus; Immunoglobulin G / analysis; Kinetics; Peptides / chemistry
TL;DR: The branched amines increased the overall sensitivity and selectivity for IgG adsorption from complex mixtures of Chinese hamster ovary supernatant (CHO) and regeneration studies were carried out. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: April 9, 2019

2019 journal article

Optimization of Sequence, Display, and Mode of Operation of IgG-Binding Peptide Ligands to Develop Robust, High-Capacity Affinity Adsorbents That Afford High IgG Product Quality

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 20(1).

author keywords: affinity chromatography; peptide ligand; antibody purification; binding capacity; process optimization; aggregate removal
TL;DR: The use of peptide ligand HWRGWV and its cognate sequences to develop affinity adsorbents that compete with Protein A in terms of binding capacity and quality of the eluted product demonstrate the potential of peptides as alternative to Protein A for the purification of therapeutic antibodies. (via Semantic Scholar)
Source: Web Of Science
Added: March 11, 2019

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