Works Published in 2021
Displaying all 9 works
Sorted by most recent date added to the index first, which may not be the same as publication date order.
2021 book
Sources: ORCID, Crossref, NC State University Libraries
Added: October 16, 2022
2021 review
Protein engineering for natural product biosynthesis and synthetic biology applications
[Review of ]. PROTEIN ENGINEERING DESIGN & SELECTION, 34.
author keywords: biosynthesis; natural products; peptides; polyketides; synthetic biology; terpenes
MeSH headings : Biocatalysis; Biological Products; Peptides / metabolism; Protein Engineering; Synthetic Biology
TL;DR:
This work details the most recent advances, focusing on polyketides, non-ribosomal peptides and isoprenoids, including their native biosynthetic logic to provide clarity for future applications of these technologies for natural product synthetic biology.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: September 27, 2021
2021 article
Development of Peptide Ligands for Targeted Capture of Host Cell Proteins from Cell Culture Production Harvests
PROTEOMIC PROFILING, 2 EDITION, Vol. 2261, pp. 489–506.
author keywords: Proteomics; Mass spectrometry; Host cell proteins; Monoclonal antibodies; Peptide ligands; Flow-through chromatography
MeSH headings : Animals; CHO Cells; Cricetulus; Enzyme-Linked Immunosorbent Assay; Humans; Ligands; Microscopy, Fluorescence; Peptide Library; Peptides / metabolism; Protein Binding; Proteins / isolation & purification; Proteins / metabolism; Proteomics; Solid Phase Extraction; Tandem Mass Spectrometry
TL;DR:
This work describes the development of HCP-binding peptide ligands, especially focusing on the steps of peptide selection via library screening and quantification of H CP removal via proteomics by mass spectrometry.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: September 20, 2021
2021 article
Mutant Construction and Integration Vector-Mediated Genetic Complementation in Listeria monocytogenes
LISTERIA MONOCYTOGENES, 2 EDITION, Vol. 2220, pp. 177–185.
author keywords: Transposon; Mutant; Listeria monocytogenes; Complementation
MeSH headings : Electroporation / methods; Genetic Vectors / genetics; Humans; Listeria monocytogenes / genetics; Listeriosis / microbiology; Mutagenesis, Insertional / methods; Mutation; Plasmids / genetics; Polymerase Chain Reaction / methods; Retroelements
TL;DR:
This chapter describes the construction and screening of mutant libraries of L. monocytogenes using the plasmid pMC38, carrying a mariner-based transposon system (TC1/mariner) and constructed by Cao et al.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 30, 2021
2021 journal article
Iminodiacetic Acid (IDA) Cation-Exchange Nonwoven Membranes for Efficient Capture of Antibodies and Antibody Fragments
MEMBRANES, 11(7).
author keywords: membrane adsorbers; membrane chromatography; nonwoven membranes; cation-exchange; UV grafting; monoclonal antibodies (mAbs); single-chain variable fragment (scFv)
TL;DR:
There is significant evidence that this weak cation-exchange nonwoven fabric platform might be a suitable alternative to packed resin chromatography for low-cost, higher productivity manufacturing of therapeutic mAbs and antibody fragments.
(via Semantic Scholar)
www.mdpi.com (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 2, 2021
2021 journal article
Hyperglycemia minimally alters primary self-renewing human colonic epithelial cells while TNF alpha-promotes severe intestinal epithelial dysfunction
INTEGRATIVE BIOLOGY, 13(6), 139–152.
author keywords: hyperglycemia; glucose; TNF alpha; colon; large intestine; intestinal epithelium; primary tissue
MeSH headings : Colon; Epithelial Cells; Humans; Hyperglycemia; Intestinal Mucosa; Tumor Necrosis Factor-alpha
TL;DR:
Results suggest that TNFα acted as the dominant modulator of the epithelium relative to glucose, and that control of inflammation rather than glucose may be key to maintaining intestinal homeostasis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: July 26, 2021
2021 journal article
Nonwoven Ion-Exchange Membranes with High Protein Binding Capacity for Bioseparations
MEMBRANES, 11(3).
author keywords: nonwoven membrane; UV-grafting; membrane chromatography; ion-exchange membrane; protein purification; membrane adsorbers
TL;DR:
The preparation and characterization of UV-grafted polybutylene terepthalate ion exchange nonwoven membranes for chromatographic purification of biomolecules indicate that these membranes have significant potential for use in downstream purifying of biology.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Source: Web Of Science
Added: April 19, 2021
2021 journal article
Structure, Function, and Thermal Adaptation of the Biotin Carboxylase Domain Dimer from Hydrogenobacter thermophilus 2-Oxoglutarate Carboxylase
BIOCHEMISTRY, 60(4), 324–345.
MeSH headings : Bacteria / enzymology; Bacterial Proteins / chemistry; Carbon-Nitrogen Ligases / chemistry; Crystallography, X-Ray; Enzyme Stability; Hot Temperature; Protein Domains; Structure-Activity Relationship
TL;DR:
Structural data reveal that the OGC BC dimer comprises a "wet" dimerization interface that is dominated by hydrophilic interactions and structural water molecules common to all BC domains and likely facilitates the conformational changes associated with the catalytic cycle.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Source: Web Of Science
Added: March 15, 2021
2021 journal article
Removal of host cell proteins from cell culture fluids by weak partitioning chromatography using peptide-based adsorbents
SEPARATION AND PURIFICATION TECHNOLOGY, 257.
author keywords: Host cell proteins; Monoclonal antibodies; Flow-through chromatography; Weak partitioning mode; Polishing; Proteomics; Peptide ligands
TL;DR:
Proteomic analysis of the effluents demonstrated that 6HP+4MP-Toyopearl adsorbent removes HCPs known for their immunogenicity or IgG co-elution or degradation, demonstrating the potential of these peptide-based resins as HCP scrubbers in mAb purification processes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Source: Web Of Science
Added: January 11, 2021
Citation Index includes data from a number of different sources. If you have questions about the sources of data in the Citation Index or need a set of data which is free to re-distribute, please contact us.
Certain data included herein are derived from the Web of Science© and InCites© (2024) of Clarivate Analytics. All rights reserved. You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.
