Golden Leaf Biomanufacturing Training and Education Center

Works Published in 2021

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Displaying all 9 works

Sorted by most recent date added to the index first, which may not be the same as publication date order.

2021 book

Biopharmaceutical Manufacturing

By: G. Gilleskie*, C. Rutter & B. McCuen

Contributors: G. Gilleskie*, C. Rutter & B. McCuen

Sources: ORCID, Crossref
Added: October 16, 2022

2021 review

Protein engineering for natural product biosynthesis and synthetic biology applications

[Review of ]. PROTEIN ENGINEERING DESIGN & SELECTION, 34.

By: M. Calzini n, A. Malico n, M. Mitchler n & G. Williams n

author keywords: biosynthesis; natural products; peptides; polyketides; synthetic biology; terpenes
MeSH headings : Biocatalysis; Biological Products; Peptides / metabolism; Protein Engineering; Synthetic Biology
TL;DR: This work details the most recent advances, focusing on polyketides, non-ribosomal peptides and isoprenoids, including their native biosynthetic logic to provide clarity for future applications of these technologies for natural product synthetic biology. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: September 27, 2021

2021 article

Development of Peptide Ligands for Targeted Capture of Host Cell Proteins from Cell Culture Production Harvests

PROTEOMIC PROFILING, 2 EDITION, Vol. 2261, pp. 489–506.

By: R. Lavoie n, T. Islam n, W. Blackburn*, R. Carbonell n & S. Menegatti n

author keywords: Proteomics; Mass spectrometry; Host cell proteins; Monoclonal antibodies; Peptide ligands; Flow-through chromatography
MeSH headings : Animals; CHO Cells; Cricetulus; Enzyme-Linked Immunosorbent Assay; Humans; Ligands; Microscopy, Fluorescence; Peptide Library; Peptides / metabolism; Protein Binding; Proteins / isolation & purification; Proteins / metabolism; Proteomics; Solid Phase Extraction; Tandem Mass Spectrometry
TL;DR: This work describes the development of HCP-binding peptide ligands, especially focusing on the steps of peptide selection via library screening and quantification of H CP removal via proteomics by mass spectrometry. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: September 20, 2021

2021 article

Mutant Construction and Integration Vector-Mediated Genetic Complementation in Listeria monocytogenes

LISTERIA MONOCYTOGENES, 2 EDITION, Vol. 2220, pp. 177–185.

author keywords: Transposon; Mutant; Listeria monocytogenes; Complementation
MeSH headings : Electroporation / methods; Genetic Vectors / genetics; Humans; Listeria monocytogenes / genetics; Listeriosis / microbiology; Mutagenesis, Insertional / methods; Mutation; Plasmids / genetics; Polymerase Chain Reaction / methods; Retroelements
TL;DR: This chapter describes the construction and screening of mutant libraries of L. monocytogenes using the plasmid pMC38, carrying a mariner-based transposon system (TC1/mariner) and constructed by Cao et al. (via Semantic Scholar)
Source: Web Of Science
Added: August 30, 2021

2021 journal article

Iminodiacetic Acid (IDA) Cation-Exchange Nonwoven Membranes for Efficient Capture of Antibodies and Antibody Fragments

MEMBRANES, 11(7).

author keywords: membrane adsorbers; membrane chromatography; nonwoven membranes; cation-exchange; UV grafting; monoclonal antibodies (mAbs); single-chain variable fragment (scFv)
TL;DR: There is significant evidence that this weak cation-exchange nonwoven fabric platform might be a suitable alternative to packed resin chromatography for low-cost, higher productivity manufacturing of therapeutic mAbs and antibody fragments. (via Semantic Scholar)
Source: Web Of Science
Added: August 2, 2021

2021 journal article

Hyperglycemia minimally alters primary self-renewing human colonic epithelial cells while TNF alpha-promotes severe intestinal epithelial dysfunction

INTEGRATIVE BIOLOGY, 13(6), 139–152.

By: J. Dutton n, S. Hinman*, R. Kim*, P. Attayek n, M. Maurer n, C. Sims*, N. Allbritton*

author keywords: hyperglycemia; glucose; TNF alpha; colon; large intestine; intestinal epithelium; primary tissue
MeSH headings : Colon; Epithelial Cells; Humans; Hyperglycemia; Intestinal Mucosa; Tumor Necrosis Factor-alpha
TL;DR: Results suggest that TNFα acted as the dominant modulator of the epithelium relative to glucose, and that control of inflammation rather than glucose may be key to maintaining intestinal homeostasis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: July 26, 2021

2021 journal article

Nonwoven Ion-Exchange Membranes with High Protein Binding Capacity for Bioseparations

MEMBRANES, 11(3).

author keywords: nonwoven membrane; UV-grafting; membrane chromatography; ion-exchange membrane; protein purification; membrane adsorbers
TL;DR: The preparation and characterization of UV-grafted polybutylene terepthalate ion exchange nonwoven membranes for chromatographic purification of biomolecules indicate that these membranes have significant potential for use in downstream purifying of biology. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: April 19, 2021

2021 journal article

Structure, Function, and Thermal Adaptation of the Biotin Carboxylase Domain Dimer from Hydrogenobacter thermophilus 2-Oxoglutarate Carboxylase

BIOCHEMISTRY, 60(4), 324–345.

By: G. Buhrman n, P. Enriquez n, L. Dillard n, H. Baer n, V. Truong n, A. Grunden n, R. Rose n

MeSH headings : Bacteria / enzymology; Bacterial Proteins / chemistry; Carbon-Nitrogen Ligases / chemistry; Crystallography, X-Ray; Enzyme Stability; Hot Temperature; Protein Domains; Structure-Activity Relationship
TL;DR: Structural data reveal that the OGC BC dimer comprises a "wet" dimerization interface that is dominated by hydrophilic interactions and structural water molecules common to all BC domains and likely facilitates the conformational changes associated with the catalytic cycle. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: March 15, 2021

2021 journal article

Removal of host cell proteins from cell culture fluids by weak partitioning chromatography using peptide-based adsorbents

SEPARATION AND PURIFICATION TECHNOLOGY, 257.

By: R. Lavoie n, W. Chu n, J. Lavoie n, Z. Hetzler n, T. Williams n, R. Carbonell n, S. Menegatti n

author keywords: Host cell proteins; Monoclonal antibodies; Flow-through chromatography; Weak partitioning mode; Polishing; Proteomics; Peptide ligands
TL;DR: Proteomic analysis of the effluents demonstrated that 6HP+4MP-Toyopearl adsorbent removes HCPs known for their immunogenicity or IgG co-elution or degradation, demonstrating the potential of these peptide-based resins as HCP scrubbers in mAb purification processes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: January 11, 2021

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