Works Published in 2001

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Displaying works 21 - 40 of 156 in total

Sorted by most recent date added to the index first, which may not be the same as publication date order.

2001 journal article

β-Mannanases from Thermotoga species

Methods in Enzymology, 330, 224–238.

By: S. Chhabra, K. Parker, D. Lam, W. Callen, M. Snead, E. Mathur, J. Short, R. Kelly*

MeSH headings : Amino Acid Sequence; Carbohydrate Conformation; Electrophoresis, Polyacrylamide Gel; Enzyme Stability; Galactose / analogs & derivatives; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / enzymology; Mannans / chemistry; Mannosidases / chemistry; Mannosidases / isolation & purification; Mannosidases / metabolism; Molecular Sequence Data; Sequence Homology, Amino Acid; beta-Mannosidase
TL;DR: This chapter describes the purification and characterization of a β -mannanase from T. neapolitana, as well as cloning and sequencing of this enzyme from T., which are useful in several industrial applications where thermostability and thermoactivity are desirable. (via Semantic Scholar)
Source: NC State University Libraries
Added: September 11, 2021

2001 report

Methods and compositions for fracturing subterranean formations

(U.S. Patent No. 6,197,730).

By: R. Kelly, S. Khan, P. Leduc, A. Tayal & R. Prud'homme

Source: NC State University Libraries
Added: September 11, 2021

2001 chapter

Homomultimeric protease and putative bacteriocin homolog from Thermotoga maritima

In Methods in Enzymology (pp. 455–460).

By: P. Hicks*, L. Chang & R. Kelly*

MeSH headings : Amino Acid Sequence; Biopolymers; Chromatography, Liquid / methods; Endopeptidases / chemistry; Endopeptidases / isolation & purification; Endopeptidases / metabolism; Microscopy, Electron; Molecular Sequence Data; Sequence Homology, Amino Acid; Thermotoga maritima / enzymology
TL;DR: Although T. maritima appears to be motile, migrating at a speed proportional to the temperature, the reduction of sulfur does not provide an energetic boost as seen by the lack of effect on growth yields and fermentation balances. (via Semantic Scholar)
Source: Crossref
Added: August 7, 2021

2001 chapter

Protease I from Pyrococcus furiosus

In Methods in Enzymology (pp. 403–413).

By: L. Chang, P. Hicks & R. Kelly*

MeSH headings : Archaeal Proteins; Chromatography, Liquid / methods; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Molecular Weight; Peptide Hydrolases / genetics; Peptide Hydrolases / isolation & purification; Peptide Hydrolases / metabolism; Pyrococcus furiosus / enzymology; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / isolation & purification; Recombinant Fusion Proteins / metabolism
TL;DR: This chapter describes several methods that have been used to purify PfpI directly from P. furiosus cell extracts, together with an assay to detect proteolytic activity, a procedure to determine its molecular mass, and approaches to minimize PfPI-catalyzed proteolysis of other P. furious proteins. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Source: Crossref
Added: August 7, 2021

2001 chapter

β-Endoglucanase from Pyrococcus furiosus

In Methods in Enzymology (pp. 346–354).

By: S. Cady n, M. Bauer, W. Callen, M. Snead, E. Mathur, J. Short, R. Kelly*

MeSH headings : Amino Acid Sequence; Base Sequence; Cellulase / biosynthesis; Cellulase / chemistry; Cellulase / isolation & purification; Cellulase / metabolism; DNA Primers; Hydrolysis; Molecular Sequence Data; Polysaccharides / chemistry; Polysaccharides / metabolism; Pyrococcus furiosus / enzymology; Recombinant Proteins / biosynthesis; Recombinant Proteins / chemistry; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Substrate Specificity
TL;DR: This chapter describes the approaches used for the cloning and expression in Escherichia coli of the eglA gene, which encodes the P. furiosus endoglucanase, and protocols used to study the biochemical properties of the recombinant enzyme. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Source: Crossref
Added: August 7, 2021

2001 chapter

αa-D-Galactosidases from Thermotoga species

In Methods in Enzymology (pp. 246–260).

By: E. Miller*, K. Parker, W. Liebl, D. Lam, W. Callen, M. Snead, E. Mathur, J. Short, R. Kelly*

MeSH headings : Base Sequence; Cloning, Molecular; DNA, Bacterial; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / isolation & purification; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / metabolism; Hydrogen-Ion Concentration; Recombinant Proteins / genetics; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Temperature; alpha-Galactosidase / genetics; alpha-Galactosidase / isolation & purification; alpha-Galactosidase / metabolism
TL;DR: This chapter discusses the purification, cloning, and expression of recombinant α Gal from T. neapolitana and T. maritima, which exhibit different biochemical properties in terms of pH optima, activity, and thermostability. (via Semantic Scholar)
Source: Crossref
Added: August 7, 2021

2001 chapter

Xylose isomerases from Thermotoga

In Methods in Enzymology (pp. 215–224).

By: C. Vieille*, D. Sriprapundh, R. Kelly* & J. Zeikus

MeSH headings : Aldose-Ketose Isomerases / chemistry; Aldose-Ketose Isomerases / genetics; Aldose-Ketose Isomerases / isolation & purification; Aldose-Ketose Isomerases / metabolism; Amino Acid Sequence; Calorimetry; Cloning, Molecular; Enzyme Stability; Escherichia coli / genetics; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / enzymology; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / growth & development; Hydrogen-Ion Concentration; Kinetics; Molecular Sequence Data
TL;DR: XIs from the hyperthermophilic eubacteria Thermotoga maritima andThermotoga neapolitana are the most thermostable yet characterized XIs. (via Semantic Scholar)
Source: Crossref
Added: August 7, 2021

2001 chapter

Carboxylesterase from Sulfolobus solfataricus P1

In Methods in Enzymology (pp. 461–471).

By: A. Sehgal n, W. Callen, E. Mathur, J. Short & R. Kelly*

MeSH headings : Amino Acid Sequence; Calorimetry, Differential Scanning; Carboxylesterase; Carboxylic Ester Hydrolases / chemistry; Carboxylic Ester Hydrolases / isolation & purification; Carboxylic Ester Hydrolases / metabolism; Electrophoresis, Polyacrylamide Gel; Molecular Sequence Data; Recombinant Proteins / chemistry; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Sulfolobus / enzymology
TL;DR: This chapter describes the cloning, expression, purification, and characterization of a recombinant carboxylesterase (Sso P1 carboxesterase) from the extreme thermoacidophile Sulfolobus solfataricus P1. (via Semantic Scholar)
Source: Crossref
Added: July 21, 2021

2001 chapter

α-Glucosidase from Pyrococcus furiosus

In Methods in Enzymology (pp. 260–269).

By: S. Chang, K. Parker, M. Bauer & R. Kelly*

MeSH headings : Chromatography, Liquid / methods; Enzyme Stability; Escherichia coli / genetics; Hydrogen-Ion Concentration; Molecular Weight; Pyrococcus furiosus / enzymology; Recombinant Proteins / isolation & purification; Recombinant Proteins / metabolism; Temperature; alpha-Glucosidases / isolation & purification; alpha-Glucosidases / metabolism
TL;DR: Despite the potential impact of hyperthermophilic enzymes on industrial processes, including starch conversion, their application is still largely unrealized, one readily apparent obstacle is developing a costefficient method for producing sufficient quantities of enzyme either directly from the source organism or through recombinant means. (via Semantic Scholar)
Source: Crossref
Added: July 21, 2021

2001 chapter

β-Mannosidase from Thermotoga species

In Methods in Enzymology (pp. 238–246).

By: K. Parker, S. Chhabra, D. Lam, M. Snead, E. Mathur & R. Kelly*

MeSH headings : Chromatography, Gel; Chromatography, Ion Exchange; Cloning, Molecular; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / enzymology; Mannosidases / genetics; Mannosidases / isolation & purification; Mannosidases / metabolism; beta-Mannosidase
TL;DR: This chapter presents the protocols used to purify the β -mannosidase from T. Neapolitana together with the results of cloning and expression of genes encoding the analogous enzyme of T. maritima in Escherichia coli. (via Semantic Scholar)
Source: Crossref
Added: July 21, 2021

2001 chapter

The Dark Side of the Force: Economic Restructuring and Social Capital in a Company Town

In B. Edwards, M. Foley, & M. Diani (Eds.), Beyond Tocqueville: Civil Society and the Social Capital Debate in Contemporary Perspective (pp. 112–124). Hanover, NH: University Press of New England.

By: M. Schulman & C. Anderson

Ed(s): B. Edwards, M. Foley & M. Diani

Source: NC State University Libraries
Added: March 26, 2021

2001 report

Risk Assessment of the Public Health Impact of Escherichia coli 0157:H7 in Ground Beef

[E. coli 0157:H7 Risk Assessment Team Report]. Washington, DC: USDA Food Safety and Inspection Service.

By: J. Kuzma

Source: NC State University Libraries
Added: March 4, 2021

2001 journal article

Helping teachers and students use advanced technology in teaching high school science: A preliminary feasibility study of the use of a WWW-controlled atomic force microscope in high school science

Technology and Teacher Education Annual, 3, 2510–2515.

By: T. Andre, M. Jones, R. Superfine & R. Taylor

Source: NC State University Libraries
Added: March 4, 2021

2001 chapter

Problems in methodology of teaching philosophy

In Filozofija – problemski pristup (pp. 206–210). Novi Sad: SSFF.

By: V. Dubljević

Source: NC State University Libraries
Added: February 27, 2021

2001 chapter

Philosophy and modern science

In Filozofija – problemski pristup (pp. 140–143). Novi Sad: SSFF.

By: V. Dubljević

Source: NC State University Libraries
Added: February 27, 2021

2001 chapter

Socrates

In Filozofija – problemski pristup (pp. 34–44). Novi Sad: SSFF.

By: V. Dubljević

Source: NC State University Libraries
Added: February 27, 2021

2001 journal article

Realistic Distributions of Infectious Periods in Epidemic Models: Changing Patterns of Persistence and Dynamics

Theoretical Population Biology, 60(1), 59–71.

By: A. Lloyd*

UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: February 22, 2021

2001 journal article

Infection dynamics on scale-free networks

Physical Review E, 64(6).

By: R. May* & A. Lloyd*

UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: ORCID
Added: February 22, 2021

2001 conference paper

The noncooperative pragmatics of arguing

In E. T. Nemeth (Ed.), Pragmatics in 2000 (pp. 263–277). Antwerp, Belgium: International Pragmatics Association.

By: J. Goodwin

Ed(s): E. Nemeth

Event: 7th International Pragmatics Conference at Budapest, Hungary

Source: NC State University Libraries
Added: February 6, 2021

2001 book

Ecological Monitoring of Genetically Modified Crops: A Workshop Summary

Jennifer Kuzma

Sources: Crossref, NC State University Libraries
Added: January 17, 2021

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