Works Published in 2016
Displaying all 7 works
Sorted by most recent date added to the index first, which may not be the same as publication date order.
2016 chapter
Aerobic Degradation of Gasoline Ether Oxygenates
In Aerobic Utilization of Hydrocarbons, Oils and Lipids.
TL;DR:
This review summarizes the main microorganisms, enzymes, and pathways involved in each of these processes and highlights research areas where there is both clear consensus and areas where results are more ambiguous and likely require further investigation.
(via Semantic Scholar)
Source: ORCID
Added: April 28, 2020
2016 journal article
The CRISPR RNA-guided surveillance complex in Escherichia coli accommodates extended RNA spacers
NUCLEIC ACIDS RESEARCH, 44(15), 7385–7394.
MeSH headings : CRISPR-Associated Proteins / metabolism; CRISPR-Cas Systems / genetics; DNA / metabolism; Escherichia coli / genetics; Escherichia coli Proteins / metabolism; Gene Expression Regulation, Bacterial; Gene Silencing; Macromolecular Substances / chemistry; Macromolecular Substances / metabolism; RNA, Bacterial / chemistry; RNA, Bacterial / genetics; RNA, Guide, Kinetoplastida / chemistry; RNA, Guide, Kinetoplastida / genetics; Transcription, Genetic
TL;DR:
It is found that extending the spacer portion of the crRNA resulted in larger Cascade complexes with altered stoichiometry and preserved in vitro binding affinity for target DNA, and spacer length can be modified to fine-tune Cascade activity.
(via Semantic Scholar)
doi.org (publisher)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
Engineering genes with CRISPR-Cas9
Chemical Engineering Progress , 112(9), 36–41.
Source: NC State University Libraries
Added: August 6, 2018
2016 review
Current and future prospects for CRISPR-based tools in bacteria
[Review of ]. BIOTECHNOLOGY AND BIOENGINEERING, 113(5), 930–943.
author keywords: antimicrobials; Cas9; genetic control; genetic circuits; genome engineering; undomesticated microbes
MeSH headings : Anti-Bacterial Agents / pharmacology; Bacteria / drug effects; Bacteria / genetics; Bacterial Infections / drug therapy; CRISPR-Cas Systems; Clustered Regularly Interspaced Short Palindromic Repeats; Drug Discovery; Drug Resistance, Bacterial; Gene Editing / methods; Gene Expression Regulation, Bacterial; Genetic Engineering / methods; Genome, Bacterial; Humans
TL;DR:
Overall, CRISPR‐Cas systems have formed the basis of an ever‐expanding genetic toolbox and hold tremendous potential for the future understanding and engineering of the bacterial world.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
Activity-Based Protein Profiling of Ammonia Monooxygenase in Nitrosomonas europaea
Appl. Environ. Microbiol., 82(8), 2270–2279.
MeSH headings : Aerobiosis; Ammonia / metabolism; Chromatography, Liquid; Mass Spectrometry; Nitrosomonas europaea / chemistry; Nitrosomonas europaea / enzymology; Oxidoreductases / analysis; Oxygen / metabolism; Proteome / analysis
TL;DR:
An activity-based protein profiling method for this enzyme using 1,7-octadiyne (17OD) as a probe is described, and peptide fragments from AmoA were the predominant peptides detected in 17OD-treated samples.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018
2016 article
Rethinking the Hierarchy of Sugar Utilization in Bacteria
Beisel, C. L., & Afroz, T. (2016, February). JOURNAL OF BACTERIOLOGY, Vol. 198, pp. 374–376.
MeSH headings : Bacteria / metabolism; Carbohydrate Metabolism / physiology; Carbohydrates / classification; Gene Expression Regulation, Bacterial
TL;DR:
Findings from studying the preferential consumption of l-arabinose over d-xylose in Escherichia coli can guide efforts to engineer the simultaneous utilization of multiple sugars.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2016 journal article
Identifying and Visualizing Functional PAM Diversity across CRISPR-Cas Systems
MOLECULAR CELL, 62(1), 137–147.
MeSH headings : Bacillus / chemistry; Bacillus / metabolism; CRISPR-Associated Proteins / chemistry; CRISPR-Associated Proteins / genetics; CRISPR-Cas Systems; Escherichia coli / chemistry; Escherichia coli / metabolism; Francisella / chemistry; Francisella / metabolism; High-Throughput Screening Assays / methods; Protein Structure, Tertiary; Streptococcus thermophilus / chemistry; Streptococcus thermophilus / metabolism
TL;DR:
An in vivo, positive, and tunable screen termed PAM-SCANR (PAM screen achieved by NOT-gate repression) to elucidate functional PAMs as well as an interactive visualization scheme termed the PAM wheel to convey individual PAM sequences and their activities to accelerate ability to understand and exploit the multitude of CRISPR-Cas systems in nature.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
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