Works Published in 2017
Displaying all 5 works
Sorted by most recent date added to the index first, which may not be the same as publication date order.
2017 journal article
Toward a genetic tool development pipeline for host-associated bacteria
Current Opinion in Microbiology, 38, 156–164.
MeSH headings : Bacteria / genetics; Gastrointestinal Microbiome; Genetics, Microbial / methods; Host-Pathogen Interactions; Humans; Molecular Biology / methods; Symbiosis
TL;DR:
The current challenges to rendering culturable, non-model members of microbial communities genetically tractable are discussed - including overcoming barriers to DNA delivery, achieving predictable gene expression, and applying CRISPR-based tools - and recent efforts to create generalized pipelines that simplify and expedite the tool-development process are details.
(via Semantic Scholar)
www.ncbi.nlm.nih.gov (repository)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Crossref
Added: February 24, 2020
2017 journal article
Advancing the design and delivery of CRISPR antimicrobials
Current Opinion in Biomedical Engineering, 4, 57–64.
author keywords: Bacteriophage; Cas9; CRISPR-Cas systems; sgRNA; Tail-fiber proteins
TL;DR:
The current efforts toward repurposing CRISPR nucleases as programmable antimicrobials may help quell the spread of antimicrobial resistance and provide a tool for the manipulation of complex microbial communities.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Crossref
Added: February 24, 2020
2017 journal article
What is the role of circuit design in the advancement of synthetic biology? Part 1 enabling real-world applications and therapeutics
Cell Systems, 4(4), 370–372.
Source: NC State University Libraries
Added: August 6, 2018
2017 journal article
Short DNA Containing chi Sites Enhances DNA Stability and Gene Expression in E-coli Cell-Free Transcription-Translation Systems
BIOTECHNOLOGY AND BIOENGINEERING, 114(9), 2137–2141.
author keywords: Escherichia coli; gene circuits; prototyping; RecBCD; synthetic biology; TXTL
MeSH headings : Cell-Free System / physiology; DNA, Bacterial / genetics; Escherichia coli / genetics; Exodeoxyribonuclease V / genetics; Gene Expression Regulation, Bacterial / genetics; Genetic Engineering / methods; Protein Biosynthesis / genetics; Transcription, Genetic / genetics
TL;DR:
It is shown that double-stranded DNA encoding χ sites-eight base-pair sequences preferentially bound by the RecBCD recombination machinery-stabilizes linear DNA and greatly enhances the TXTL-based expression and activity of a fluorescent reporter gene, simple regulatory cascades, and T7 bacteriophage particles.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2017 review
Deciphering, Communicating, and Engineering the CRISPR PAM
[Review of ]. JOURNAL OF MOLECULAR BIOLOGY, 429(2), 177–191.
author keywords: Cas9; Cpf1; CRISPR-Cas systems; PFS; rPAM
MeSH headings : Amino Acid Motifs; CRISPR-Cas Systems; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; DNA, Bacterial / genetics; Gene Library; Genetic Loci; Plasmids / genetics; Protein Engineering; RNA / genetics; RNA / metabolism; Staphylococcus epidermidis / genetics; Streptococcus thermophilus / genetics
TL;DR:
The properties of the CRISPR PAM and the emerging tools for determining, visualizing, and engineering PAM recognition are discussed and a standard means of orienting the PAM is proposed to simplify how its location and sequence are communicated.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
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