TY - JOUR TI - Identification of sucrose synthase as an actin-binding protein AU - Winter, H. AU - L. Huber, J. AU - Huber, S.C. T2 - FEBS Letters AB - Several lines of evidence indicate that sucrose synthase (SuSy) binds both G- and F-actin: (i) presence of SuSy in the Triton X-100-insoluble fraction of microsomal membranes (i.e. crude cytoskeleton fraction); (ii) co-immunoprecipitation of actin with anti-SuSy monoclonal antibodies; (iii) association of SuSy with in situ phalloidin-stabilized F-actin filaments; and (iv) direct binding to F-actin, polymerized in vitro. Aldolase, well known to interact with F-actin, interfered with binding of SuSy, suggesting that a common or overlapping binding site may be involved. We postulate that some of the soluble SuSy in the cytosol may be associated with the actin cytoskeleton in vivo. DA - 1998/// PY - 1998/// DO - 10.1016/S0014-5793(98)00659-0 VL - 430 IS - 3 SP - 205-208 UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-0032479338&partnerID=MN8TOARS KW - actin-binding protein KW - actin cytoskeleton KW - aldolase KW - sucrose synthase KW - phalloidin ER - TY - JOUR TI - Growth dynamics and cytoskeleton organization during stem maturation and gravity-induced stem bending in Zea mays L. AU - Collings, D.A. AU - Winter, H. AU - Wyatt, S.E. AU - Allen, N.S. T2 - Planta DA - 1998/// PY - 1998/// DO - 10.1007/s004250050480 VL - 207 IS - 2 SP - 246-258 UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-0032435367&partnerID=MN8TOARS KW - actin KW - cell elongation KW - gravitropism KW - microtubule KW - pulvinus KW - Zea (gravitropism) ER -