Works Published in 2018

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Displaying all 8 works

Sorted by most recent date added to the index first, which may not be the same as publication date order.

2018 journal article

Metabolic and Transcriptional Profiles of Dunaliella viridis Supplemented With Ammonium Derived From Glutamine

FRONTIERS IN MARINE SCIENCE, 5(AUG).

Contributors: J. Dums n, C. Murphree n, N. Vasani n, D. Young n & H. Sederoff n

author keywords: Dunaliella; nitrogen starvation; ammonium; amino acids; glutamine; biofuel; nitrogen recycling; transcriptome
TL;DR: The results indicate that a continuous, amino acid derived slow release of NH4+ to algae cultures could reduce the amount of synthetic nitrogen needed for growth, but optimization is needed to balance nitrogen starvation and cell division. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: February 18, 2019

2018 conference paper

Tracking Gene Expression via Light Sheet Microscopy and Computer Vision in Living Organisms

2018 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), 818–821.

By: E. Buckner n, C. Ottley n, C. Williams n, A. Luis Balaguer n, C. Melvin n & R. Sozzani n

Event: 2018 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)

MeSH headings : Arabidopsis; Cell Nucleus; Microscopy; Proteins
TL;DR: The solution indicates, segments, and tracks gene expression using a fluorescent imaging channel of a light sheet microscope while determining gene expression location within an organism from a Brightfield (non-fluorescent) imaging channel. (via Semantic Scholar)
Sources: NC State University Libraries, NC State University Libraries
Added: February 8, 2019

2018 chapter

Identifying Differentially Expressed Genes Using Fluorescence-Activated Cell Sorting (FACS) and RNA Sequencing from Low Input Samples

In L. von Stechow & A. Santos Delgado (Eds.), Computational Cell Biology (pp. 139–151).

By: N. Clark n, A. Fisher n & R. Sozzani n

Ed(s): L. Stechow & A. Santos Delgado

author keywords: Protoplasting; Fluorescence activated cell sorting; RNA sequencing; Differential expression; Low input samples
MeSH headings : Arabidopsis / cytology; Arabidopsis / genetics; Arabidopsis / metabolism; Cytoplasm / metabolism; Flow Cytometry / methods; Gene Expression Regulation, Plant / physiology; Plant Roots / cytology; Plant Roots / genetics; Plant Roots / metabolism; RNA, Plant / biosynthesis; RNA, Plant / genetics; Sequence Analysis, RNA / methods
TL;DR: FACS is described to perform fluorescence-activated cell sorting on Arabidopsis root protoplasts to isolate specific cell types in the root and how to extract and process RNA from a very low number of cells for RNA sequencing (RNA seq). (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: February 8, 2019

2018 journal article

Grafting the Indeterminate Tomato Cultivar Moneymaker onto Multifort Rootstock Improves Cold Tolerance

HORTSCIENCE, 53(11), 1610–1617.

By: D. Suchoff n, P. Perkins-Veazie n, H. Sederoff n, J. Schultheis n, M. Kleinhenz*, F. Louws n, C. Gunter n

Contributors: D. Suchoff n, P. Perkins-Veazie n, H. Sederoff n, J. Schultheis n, M. Kleinhenz*, F. Louws n, C. Gunter n

author keywords: Solanum habrochaites; Solanum lycopersicum; herbaceous grafts; root system morphology; suboptimal temperature
TL;DR: The ability for ‘Multifort’ to maintain root growth at suboptimal temperatures may improve root system sink strength, thereby promoting movement of photosynthate from leaf to root even under cold conditions. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
7. Affordable and Clean Energy (OpenAlex)
13. Climate Action (Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: December 17, 2018

2018 journal article

A microfluidic platform for lifelong high-resolution and high throughput imaging of subtle aging phenotypes in C-elegans

LAB ON A CHIP, 18(20).

By: S. Saberi-Bosari n, J. Huayta n & A. San-Miguel n

MeSH headings : Aging; Animals; Caenorhabditis elegans / cytology; Caenorhabditis elegans / physiology; Equipment Design; Lab-On-A-Chip Devices; Molecular Imaging / instrumentation; Phenotype; Temperature
TL;DR: Computer vision was implemented to perform automated unbiased detection of synapses and quantitative analysis of aging-induced synaptic changes, and was able to perform periodic high-resolution microscopy of fluorescently tagged synapses located at the dorsal side of the nematode's tail throughout the worms' lifespan. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, ORCID, NC State University Libraries
Added: November 12, 2018

2018 journal article

Molecular Dynamics Simulations of Kir2.2 Interactions with an Ensemble of Cholesterol Molecules

BIOPHYSICAL JOURNAL, 115(7), 1264–1280.

By: N. Barbera*, M. Ayee*, B. Akpa n & I. Levitan*

Contributors: N. Barbera*, M. Ayee*, B. Akpa n & I. Levitan*

MeSH headings : Binding Sites; Cholesterol / metabolism; Elasticity; Ion Channel Gating; Molecular Dynamics Simulation; Potassium Channels, Inwardly Rectifying / chemistry; Potassium Channels, Inwardly Rectifying / metabolism; Protein Binding; Protein Conformation
TL;DR: Martini coarse-grained simulations are used to analyze the long timescale dynamics of cholesterol with Kir2.2 channels and find that the cholesterol environment surrounding Kir channels forms a complex milieu of different short- and long-term interactions, with multiple cholesterol molecules concurrently interacting with the channel. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries, ORCID
Added: October 16, 2018

2018 journal article

Exposure to heavy metal stress triggers changes in plasmodesmatal permeability via deposition and breakdown of callose

JOURNAL OF EXPERIMENTAL BOTANY, 69(15), 3715–3728.

By: R. O'Lexy*, K. Kasai*, N. Clark n, T. Fujiwara*, R. Sozzani n & K. Gallagher*

author keywords: Callose; copper; iron; nutrient stress; plasmodesmata; root development
MeSH headings : Arabidopsis / drug effects; Arabidopsis / physiology; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Glucan 1,3-beta-Glucosidase / genetics; Glucan 1,3-beta-Glucosidase / metabolism; Glucans / metabolism; Glucosyltransferases / genetics; Glucosyltransferases / metabolism; Metals, Heavy / toxicity; Permeability / drug effects; Plant Roots / drug effects; Plant Roots / physiology; Plasmodesmata / drug effects; Plasmodesmata / metabolism
TL;DR: It is shown that significant changes in plasmodesmatal permeability underlie root responses to nutrient stress and this results in new insights into how plants modify their growth to adapt to changes in their environment. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2018 journal article

GTL1 and DF1 regulate root hair growth through transcriptional repression of ROOT HAIR DEFECTIVE 6-LIKE 4 in Arabidopsis

DEVELOPMENT, 145(3).

author keywords: Root hair; Cell growth; Trihelix transcription factor; Gene regulatory network
MeSH headings : Arabidopsis / genetics; Arabidopsis / growth & development; Arabidopsis / metabolism; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Basic Helix-Loop-Helix Transcription Factors / genetics; Basic Helix-Loop-Helix Transcription Factors / metabolism; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Gene Regulatory Networks; Genes, Plant; Indoleacetic Acids / metabolism; Models, Biological; Mutation; Plant Growth Regulators / metabolism; Plant Roots / growth & development; Plant Roots / metabolism; Plants, Genetically Modified; Promoter Regions, Genetic; Signal Transduction; Transcription Factors / genetics; Transcription Factors / metabolism; Transcription, Genetic
TL;DR: This study uncovers a core regulatory module that fine-tunes the extent of root hair growth by the orchestrated actions of opposing transcription factors. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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