Works Published in 1998

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Displaying all 4 works

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1998 journal article

Isolation, characterization, and polymorphism detection of the porcine apolipoprotein E gene

Animal Genetics, 29, 43–47.

By: J. Ramsoondar*, J. Vazques, E. Rucker*, R. Dickson, J. Lunney & J. Piedrahita*

MeSH headings : Animals; Apolipoproteins E / chemistry; Apolipoproteins E / genetics; Apolipoproteins E / isolation & purification; Base Sequence; Chromosome Mapping / veterinary; Cloning, Molecular; DNA Primers; Disease Models, Animal; Exons / genetics; Gene Frequency; In Situ Hybridization, Fluorescence / veterinary; Introns / genetics; Microsatellite Repeats / genetics; Molecular Sequence Data; Protein Sorting Signals / genetics; Restriction Mapping / veterinary; Sequence Analysis, DNA / veterinary; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Swine / genetics
TL;DR: The present report describes the isolation and genetic characterization of the porcine apolipoprotein E (apo-E) gene, which contains a (CG)13 microsatellite marker within intron three that is moderately polymorphic. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018

1998 journal article

Generation of transgenic porcine chimeras using primordial germ cell-derived colonies

BIOLOGY OF REPRODUCTION, 58(5), 1321–1329.

By: J. Piedrahita*, K. Moore*, B. Oetama*, C. Lee*, N. Scales*, J. Ramsoondar*, F. Bazer*, T. Ott*

MeSH headings : Animals; Animals, Genetically Modified / genetics; Animals, Genetically Modified / physiology; Blotting, Southern; Cell Differentiation / physiology; Cells, Cultured; Chimera / genetics; Female; Germ Cells / physiology; Pregnancy; Swine; Transformation, Genetic
TL;DR: Results indicating that porcine EG cell lines can be isolated, genetically transformed, and utilized to make transgenic chimeras are presented. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

1998 journal article

Factors affecting the efficiency of introducing precise genetic changes in ES cells by homologous recombination: tag-and-exchange versus the Cre-loxp system


By: J. Vazquez*, C. Nogues*, E. Rucker* & J. Piedrahita*

author keywords: gene targeting; homologous recombination; Cre-LoxP; site-specific recombination
MeSH headings : Animals; Apolipoproteins E / genetics; Cells, Cultured; Electroporation; Embryo, Mammalian / cytology; Gene Targeting; Genetic Markers; Genetic Vectors; Integrases / metabolism; Mice; Milk Proteins / genetics; Mutation; Plasmids; Recombination, Genetic; Selection, Genetic; Stem Cells; Thymidine Kinase / genetics; Viral Proteins
TL;DR: This work analysed how the distance between two gene markers placed within the region of homology of a targeting vector affects the rate at which both markers are introduced into the locus during the homologous recombination event. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

1998 journal article

Cellular differentiation and I-FABP protein expression modulate fatty acid uptake and diffusion


By: B. Atshaves, W. Foxworth, A. Frolov, J. Roths, A. Kier, B. Oetama, J. Piedrahita*, F. Schroeder

author keywords: embryonic stem cells; intracellular localization; laser cytometry; fluorescence recovery after photobleaching; intestinal fatty acid binding protein
MeSH headings : 4-Chloro-7-nitrobenzofurazan / analogs & derivatives; 4-Chloro-7-nitrobenzofurazan / pharmacokinetics; Animals; Carrier Proteins / biosynthesis; Carrier Proteins / genetics; Cell Differentiation; Cells, Cultured; Clone Cells / metabolism; Diffusion; Fatty Acid-Binding Protein 7; Fatty Acid-Binding Proteins; Fatty Acids / metabolism; Indicators and Reagents / pharmacokinetics; Mice; Myelin P2 Protein / biosynthesis; Myelin P2 Protein / genetics; Neoplasm Proteins; Nerve Tissue Proteins; Polymerase Chain Reaction; Restriction Mapping; Stearates / pharmacokinetics; Stem Cells / metabolism; Transfection
Source: Web Of Science
Added: August 6, 2018

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