Molecular Education, Technology and Research Innovation Center

Works Published in 2000

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Sorted by most recent date added to the index first, which may not be the same as publication date order.

2000 journal article

An experimental and theoretical study of the gas-phase decomposition of monoprotonated peptide nucleic acids

Journal of the American Society for Mass Spectrometry, 11(7), 615–625.

By: J. Flora*, D. Shillady* & D. Muddiman* 

MeSH headings : Acetylation; Cyclotrons; Fourier Analysis; Gas Chromatography-Mass Spectrometry; Molecular Weight; Peptide Nucleic Acids / chemistry; Protons; Terminology as Topic
TL;DR: Electrospray ionization coupled with Fourier transform ion cyclotron resonance mass spectrometry shows great potential as a tool for the characterization and structural elucidation of these oligonucleotides. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 27, 2021

2000 journal article

A dual electrospray ionization source combined with hexapole accumulation to achieve high mass accuracy of biopolymers in Fourier transform ion cyclotron resonance mass spectrometry

Journal of the American Society for Mass Spectrometry, 11(10), 876–883.

By: J. Hannis* & D. Muddiman* 

MeSH headings : Biopolymers / chemistry; Calibration; Cyclotrons; Fourier Analysis; Mass Spectrometry; Reverse Transcriptase Polymerase Chain Reaction; Spectrophotometry, Ultraviolet
TL;DR: An ESI source employed with hexapole accumulation and gated trapping provides a novel method of using an internal standard to achieve high mass accuracies in Fourier transform ion cyclotron resonance mass spectrometry, illustrating this method’s use for characterizing large biomolecules such as those encountered in genomics and proteomics related research. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2000 journal article

Preparation of single-stranded PCR products for electrospray ionization mass spectrometry using the DNA repair enzyme lambda exonuclease

The Analyst, 125(4), 619–626.

By: A. Null*, J. Hannis* & D. Muddiman* 

MeSH headings : Base Sequence; DNA, Single-Stranded; Exodeoxyribonucleases; Mass Spectrometry; Molecular Sequence Data; Polymerase Chain Reaction / methods; Tyrosine 3-Monooxygenase / genetics; Viral Proteins
TL;DR: Lambda exonuclease clearly provides mass spectrometrists with a method to generate single-stranded PCR products and shows that one strand is preferentially adenylated over the other, which cannot be determined from the mass spectrum of the double-Stranded PCR product alone. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 12, 2021

2000 entry reference

Absolute Quantification of Proteins by Mass Spectrometry

By: C. Shuford  n & D. Muddiman n 

TL;DR: All phases of the PC-IDMS workflow, from the SIL peptide production platforms to data analysis, are described and characterized in detail and an emphasis is placed on the practical quantitative aspects for each step of the workflow and their implication on quantitative accuracy. (via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: January 18, 2021

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