Molecular Education, Technology and Research Innovation Center

Works Published in 2003

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Displaying all 11 works

Sorted by most recent date added to the index first, which may not be the same as publication date order.

2003 conference paper

Analysis of Aldehydes and Aldehyde-DNA Adducts by HPLC Tandem Mass Spectrometry

Presented at the Naff Symposium on Chemistry and Molecular Biology, University of Kentucky, Lexington, KY.

By: T. Williams, X. Liu & B. Lynn

Event: Naff Symposium on Chemistry and Molecular Biology at University of Kentucky, Lexington, KY

Source: NC State University Libraries
Added: March 6, 2022

2003 journal article

Cancer Biomarkers: How Proteomics is Leading to the Discovery of New Markers

Clinical Laboratory News, 29, 12–16.

By: D. Muddiman, W. Cliby & H. Bergen

Source: NC State University Libraries
Added: March 17, 2021

2003 journal article

Enzymatic strategies for the characterization of nucleic acids by electrospray ionization mass spectrometry

Rapid Communications in Mass Spectrometry, 17(24), 2699–2706.

By: A. Null*, L. Benson* & D. Muddiman*

MeSH headings : Exodeoxyribonucleases / chemistry; Isotope Labeling / methods; Nucleic Acids / analysis; Nucleic Acids / chemistry; Oligonucleotides / analysis; Oligonucleotides / chemistry; Polymerase Chain Reaction / methods; Polymorphism, Single Nucleotide; Reproducibility of Results; Restriction Mapping / methods; Sensitivity and Specificity; Sequence Alignment / methods; Sequence Analysis, DNA / methods; Spectrometry, Mass, Electrospray Ionization / methods; Viral Proteins
TL;DR: This work describes an eclectic array of enzymatic strategies invoked in order to detect single-nucleotide polymorphisms by ESI-MS, although other applications may be envisioned. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 27, 2021

2003 journal article

1H NMR and electrospray mass spectrometry of the mono-ionized bis(2,2′-bis(4,5-dimethylimidazole)chloronitrosylruthenium(II) complex ([Ru(NO)Cl(LH2)2]+, LH2=2,2′-bis(4,5-dimethylimidazole)

Inorganica Chimica Acta, 343, 317–328.

By: T. Stringfield*, K. Somayajula*, D. Muddiman*, J. Flora* & R. Shepherd*

author keywords: ruthenium complexes; nitrosyl complexes; imidazole complexes; electrospray mass spectrometry
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Sources: Crossref, ORCID
Added: February 27, 2021

2003 journal article

Naturally processed measles virus peptide eluted from class II HLA-DRB1*03 recognized by T lymphocytes from human blood

Virology, 312(2), 495–506.

By: I. Ovsyannikova*, K. Johnson*, S. Naylor*, D. Muddiman* & G. Poland*

author keywords: measles-derived peptide; HLA-DRB1*03; measles virus; mass spectrometry; lymphocyte proliferation
MeSH headings : Adolescent; Amino Acid Sequence; Antigen Presentation; Antigens, Viral / analysis; Antigens, Viral / chemistry; Antigens, Viral / immunology; Antigens, Viral / metabolism; Child; Female; HLA-DR Antigens / immunology; HLA-DR Antigens / metabolism; HLA-DRB1 Chains; Humans; Male; Mass Spectrometry; Measles Vaccine / immunology; Measles virus / chemistry; Measles virus / metabolism; Molecular Sequence Data; Peptides / analysis; Peptides / chemistry; Peptides / immunology; Peptides / metabolism; T-Lymphocytes / immunology
TL;DR: This data provides direct evidence that the antigenic peptide of measles virus was processed by antigen-presenting cells, presented in the context of HLA class II molecules, and was recognized by peripheral blood T cells from healthy individuals previously immunized with measles vaccine. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Sources: Crossref, ORCID
Added: February 12, 2021

2003 journal article

Coordination-Driven Self-Assembly of Supramolecular Cages:  Heteroatom-Containing and Complementary Trigonal Prisms

Journal of the American Chemical Society, 125(32), 9647–9652.

By: Y. Kryschenko*, S. Seidel*, D. Muddiman*, A. Nepomuceno* & P. Stang*

MeSH headings : Anthracenes / chemical synthesis; Anthracenes / chemistry; Benzene Derivatives / chemical synthesis; Benzene Derivatives / chemistry; Cross-Linking Reagents / chemical synthesis; Cross-Linking Reagents / chemistry; Crystallography, X-Ray; Fourier Analysis; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Structure; Organophosphorus Compounds / chemical synthesis; Organophosphorus Compounds / chemistry; Organosilicon Compounds / chemical synthesis; Organosilicon Compounds / chemistry; Spectrometry, Mass, Electrospray Ionization
TL;DR: The self-assembly of three nanoscopic prisms of approximate size 1 x 4 nm is reported, which completes a pair of nanoscopic complementary 3-D structures. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021

2003 journal article

Dual Electrospray Ionization Source for Confident Generation of Accurate Mass Tags Using Liquid Chromatography Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Analytical Chemistry, 75(14), 3411–3418.

By: A. Nepomuceno*, D. Muddiman*, H. Bergen*, J. Craighead*, M. Burke*, P. Caskey*, J. Allan*

MeSH headings : Amino Acid Sequence; Apoproteins / chemistry; Chromatography, Liquid; Cyclotrons; Mass Spectrometry; Molecular Sequence Data; Myoglobin / chemistry; Proteins / chemistry; Proteomics / instrumentation; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectroscopy, Fourier Transform Infrared / instrumentation
TL;DR: It is argued that the most effective way to account for space-charge effects is to introduce an internal mass calibrant (IMC) using a dual electrospray ionization (ESI) source where the IMC is added from a separate ESI emitter. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021

2003 journal article

Determination of a correction to improve mass measurement accuracy of isotopically unresolved polymerase chain reaction amplicons by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry

Rapid Communications in Mass Spectrometry, 17(15), 1714–1722.

By: A. Null* & D. Muddiman*

MeSH headings : Humans; Molecular Weight; Nucleic Acids / analysis; Polymerase Chain Reaction; Proteins / analysis; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods; Spectroscopy, Fourier Transform Infrared / instrumentation; Spectroscopy, Fourier Transform Infrared / methods; Tandem Repeat Sequences / genetics
TL;DR: A practical correction factor is developed that is applied to the ToC measurement to largely remove the systematic bias introduced by nature and accurately predicts resolving power achievable for Fourier transform ion cyclotron resonance (FT-ICR) mass analysis of large molecules. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021

2003 journal article

Mass spectral determination of skeletal/cardiac actin isoform ratios in cardiac muscle

Rapid Communications in Mass Spectrometry, 17(13), 1467–1471.

By: H. Bergen*, K. Ajtai*, T. Burghardt*, A. Nepomuceno* & D. Muddiman*

MeSH headings : Actins / analysis; Animals; Cattle; Mass Spectrometry; Muscle, Skeletal / chemistry; Myocardium / chemistry; Protein Isoforms / analysis; Rabbits
TL;DR: A mass spectrometric technique to measure the ratio of skeletal to cardiac actin and a new dual electrospray ionization source was employed to determine accurate masses of the alpha-skeletal and alpha-cardiac actins. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021

2003 journal article

Advantages of Thermococcus kodakaraenis (KOD) DNA Polymerase for PCR-mass spectrometry based analyses

Journal of the American Society for Mass Spectrometry, 14(6), 601–604.

By: L. Benson*, A. Null* & D. Muddiman*

MeSH headings : DNA-Directed DNA Polymerase / chemistry; Fourier Analysis; Humans; Polymerase Chain Reaction / methods; Spectrometry, Mass, Electrospray Ionization; Taq Polymerase / chemistry; Thermococcus / enzymology; von Willebrand Factor / chemistry
TL;DR: KOD DNA polymerase demonstrated a 2- to 3-fold increase in PCR product formation compared to Pfu or Taq, respectively, and generated blunt-ended PCR product which allows facile interpretation of the mass spectrum. (via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021

2003 journal article

Implications of Hydrophobicity and Free Energy of Solvation for Characterization of Nucleic Acids by Electrospray Ionization Mass Spectrometry

Analytical Chemistry, 75(6), 1331–1339.

By: A. Null*, A. Nepomuceno* & D. Muddiman*

MeSH headings : Deoxyribonucleotides / analysis; Gene Frequency; Hydrophobic and Hydrophilic Interactions; Nucleic Acids / chemistry; Oligonucleotides / analysis; Polymorphism, Single Nucleotide; Solvents; Spectrometry, Mass, Electrospray Ionization / methods; Thermodynamics
TL;DR: Investigation of the contributions of hydrophobicity and free energy of solvation to relative signal intensities in ESI-MS spectra of nucleic acids found a trend in signal intensity for deoxyribonucleotide triphosphates, oligonucleotides, and PCR amplicons was observed that was consistent with the model. (via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy (OpenAlex)
Sources: Crossref, ORCID
Added: February 12, 2021

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