Works Published in 2003

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Displaying works 21 - 40 of 62 in total

Sorted by most recent date added to the index first, which may not be the same as publication date order.

2003 journal article

NMR analysis of lignins in CAD-deficient plants. Part 1. Incorporation of hydroxycinnamaldehydes and hydroxybenzaldehydes into lignins

ORGANIC & BIOMOLECULAR CHEMISTRY, 1(2), 268–281.

By: H. Kim*, J. Ralph*, F. Lu*, S. Ralph*, A. Boudet*, J. MacKay*, R. Sederoff n, T. Ito* ...

MeSH headings : Alcohol Oxidoreductases / deficiency; Alcohol Oxidoreductases / genetics; Benzaldehydes / chemistry; Benzaldehydes / metabolism; Carbon Isotopes; Cinnamates / chemistry; Cinnamates / metabolism; Dimerization; Down-Regulation; Lignin / biosynthesis; Lignin / chemistry; Nuclear Magnetic Resonance, Biomolecular; Pinus / enzymology; Pinus / genetics; Pinus / metabolism; Plants, Genetically Modified / enzymology; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Tobacco / enzymology; Tobacco / genetics; Tobacco / metabolism
TL;DR: Spectral data from dimers, oligomers, and synthetic polymers have allowed a more substantive assignment of aldehyde components in lignins isolated from a CAD-deficient pine mutant and an antisense-CAD-downregulated transgenic tobacco, suggesting that the aldehydes behave like the traditional monolignols and should probably be regarded as authentic lignin monomers in normal and CAD- deficient plants. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Morphological variants of Sindbis virus produced by a mutation in the capsid protein

VIROLOGY, 307(1), 54–66.

By: D. Ferreira n, R. Hernandez n, M. Horton n & D. Brown n

MeSH headings : Animals; Capsid Proteins / genetics; Cell Line; Cricetinae; Kidney; Microscopy, Electron; Mutation; RNA, Viral / genetics; Sindbis Virus / genetics; Sindbis Virus / physiology; Sindbis Virus / ultrastructure; Transcription, Genetic; Transfection; Viral Plaque Assay
TL;DR: The data presented in the following manuscript show that mutations in the capsid protein can result in the assembly of the virus structural proteins into icosahedra of different triangulation numbers, and support the hypothesis that families of icosadeltahedra would be developed by altering the distance between the points of insertion of the five-fold axis. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Investigating the possibility of monitoring lectin levels in commercial soybean meals intended for poultry feeding using steam-heated soybean meal as a model

POULTRY SCIENCE, 82(4), 648–656.

By: Y. Fasina*, H. Classen*, J. Garlich*, H. Swaisgood* & D. Clare*

author keywords: soybean meal; lectin; urease activity; trypsin inhibitor; protein solubility
MeSH headings : Animal Feed / analysis; Animal Nutritional Physiological Phenomena; Animals; Food Handling / methods; Hydrogen-Ion Concentration; Nutritive Value; Plant Lectins / analysis; Plant Lectins / metabolism; Poultry / metabolism; Soybean Proteins / analysis; Soybean Proteins / metabolism; Temperature; Time Factors; Trypsin Inhibitors / analysis; Urease / metabolism
TL;DR: The denaturation pattern of UA was highly correlated with that of SBL, indicating that UA could be used for monitoring lectin levels in commercial meals, and it was concluded that UA of 0.03 to 0.09 units of pH change are indicative of adequately processed meals that contain negligible Lectin levels. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Heterozygosity for ABCA1 gene mutations: effects on enzymes, apolipoproteins and lipoprotein particle size

ATHEROSCLEROSIS, 171(2), 311–319.

By: J. Kuivenhoven*, G. Hovingh*, A. Tol*, M. Jauhiainen*, C. Ehnholm*, J. Fruchart*, E. Brinton*, J. Otvos n ...

author keywords: HDL; LCAT; CETP; PLTP; ABCA1
MeSH headings : ATP Binding Cassette Transporter 1; ATP-Binding Cassette Transporters / genetics; Analysis of Variance; Apolipoproteins / analysis; Apolipoproteins / metabolism; Case-Control Studies; Codon, Nonsense; Cohort Studies; Female; Follow-Up Studies; Heterozygote; Humans; Hyperlipoproteinemia Type II / epidemiology; Hyperlipoproteinemia Type II / genetics; Hyperlipoproteinemia Type II / metabolism; Lipoproteins / analysis; Lipoproteins / metabolism; Male; Multivariate Analysis; Mutation, Missense; Particle Size; Phosphatidylcholine-Sterol O-Acyltransferase / analysis; Phosphatidylcholine-Sterol O-Acyltransferase / metabolism; Probability; Reference Values; Risk Assessment
TL;DR: Heterozygosity for ABCA1 defects was associated with reduced LCAT activity in absence of effects on PLTP activity, and the effects of compromisedABCA1 function on HDL were more pronounced in women than in men. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Organic solvents order the dynamic switch II in Ras crystals

STRUCTURE, 11(7), 747–751.

By: G. Buhrman n, V. Serrano n & C. Mattos n

MeSH headings : Crystallization; Guanosine Triphosphate / analogs & derivatives; Guanosine Triphosphate / chemistry; Organic Chemicals / chemistry; Solvents / chemistry; ras Proteins / chemistry
TL;DR: The results suggest that hydrophobic solvents can be used in general to order short biologically relevant segments of disordered regions in protein crystals by favoring H-bonding interactions between atoms that are highly solvated and mobile in aqueous solution. (via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Mutations in the procaspase-3 dimer interface affect the activity of the zymogen

BIOCHEMISTRY, 42(42), 12311–12320.

By: C. Pop n, B. Feeney n, A. Tripathy n & A. Clark n

MeSH headings : Base Sequence; Caspase 3; Caspases / chemistry; Caspases / genetics; Caspases / metabolism; DNA Primers; Dimerization; Enzyme Precursors / chemistry; Enzyme Precursors / genetics; Enzyme Precursors / metabolism; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Protein Conformation; Spectrometry, Fluorescence
TL;DR: While the mutations do not affect the dimeric properties of the procaspase, it is shown that the V266E mutation may affect the formation of a loop bundle that is important for stabilizing the active site. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Lipoproteins in the DCCT/EDIC cohort: Associations with diabetic nephropathy

KIDNEY INTERNATIONAL, 64(3), 817–828.

By: A. Jenkins, T. Lyons, D. Zheng, J. Otvos*, D. Lackland, D. McGee, W. Garvey, R. Klein

author keywords: diabetes mellitus; nephropathy; lipoproteins; NMR
MeSH headings : Adolescent; Adult; Albuminuria; Child; Cholesterol, LDL / blood; Cohort Studies; Creatinine / blood; Cross-Sectional Studies; Diabetic Nephropathies / blood; Diabetic Nephropathies / urine; Female; Humans; Lipoproteins / blood; Lipoproteins, IDL; Lipoproteins, LDL / blood; Lipoproteins, VLDL / blood; Magnetic Resonance Spectroscopy; Male; Multivariate Analysis; Sex Characteristics; Triglycerides / blood
TL;DR: Potentially atherogenic lipoprotein profiles are associated with renal dysfunction in type 1 diabetes and further details are gained from NMR analysis. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Characterisation of PtMYB1, an R2R3-MYB from pine xylem

PLANT MOLECULAR BIOLOGY, 53(4), 597–608.

author keywords: MYB; phenylalanine ammonia-lyase; phenylpropanoid; pine; xylem
MeSH headings : Adenosine Triphosphate / genetics; Adenosine Triphosphate / metabolism; Base Sequence; Binding Sites / genetics; Cloning, Molecular; Cytidine Triphosphate / genetics; Cytidine Triphosphate / metabolism; DNA, Complementary / chemistry; DNA, Complementary / genetics; Electrophoretic Mobility Shift Assay; Molecular Sequence Data; Oligonucleotides / genetics; Oligonucleotides / metabolism; Phylogeny; Pinus / genetics; Plant Proteins / genetics; Plant Proteins / metabolism; Plant Structures / genetics; Protein Binding; Proto-Oncogene Proteins c-myb / genetics; Proto-Oncogene Proteins c-myb / metabolism; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Saccharomyces cerevisiae / genetics; Sequence Analysis, DNA; Transcription, Genetic / genetics
TL;DR: Rec recombinant PtMYB1 protein was able to bind to AC elements and induce AC-element-dependent shifts in the electrophoretic mobility of a plant promoter that contains three AC elements, the minimal PHENYLALANINE AMMONIA-LYASE 2 (PAL2) promoter from Phaseolus vulgaris. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Bioimmobilization of keratinase using Bacillus subtilis and Escherichia coli systems

BIOTECHNOLOGY AND BIOENGINEERING, 81(4), 421–429.

By: J. Wang n, H. Swaisgood n & J. Shih n

author keywords: keratinase; immobilization; streptavidin; fusion protein
MeSH headings : Bacillus subtilis / classification; Bacillus subtilis / enzymology; Bacillus subtilis / genetics; Cells, Cultured; Enzymes, Immobilized / chemical synthesis; Enzymes, Immobilized / genetics; Enzymes, Immobilized / isolation & purification; Enzymes, Immobilized / metabolism; Escherichia coli / classification; Escherichia coli / enzymology; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Macromolecular Substances; Peptide Hydrolases / classification; Peptide Hydrolases / genetics; Peptide Hydrolases / isolation & purification; Peptide Hydrolases / metabolism; Protein Binding; Protein Engineering / methods; Recombinant Fusion Proteins / biosynthesis; Recombinant Fusion Proteins / genetics; Species Specificity; Streptavidin / biosynthesis; Streptavidin / classification; Streptavidin / genetics; Streptavidin / isolation & purification
TL;DR: A new genetic construct was developed to produce the keratinase-streptavidin fusion protein, so that the purification and immobilization of the fusion protein onto a biotinylated matrix can be accomplished in a single step. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Sub-classification of response regulators using the surface characteristics of their receiver domains

FEBS LETTERS, 554(3), 231–236.

author keywords: OmpR sub-families; homology modeling
MeSH headings : Bacillus subtilis / chemistry; Bacillus subtilis / metabolism; Bacterial Proteins / chemistry; Bacterial Proteins / classification; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Escherichia coli / chemistry; Escherichia coli / metabolism; Genes, Regulator; Histidine Kinase; Hydrophobic and Hydrophilic Interactions; Models, Molecular; Protein Kinases / genetics; Protein Kinases / metabolism; Protein Structure, Secondary; Protein Structure, Tertiary; Structural Homology, Protein; Trans-Activators / chemistry; Trans-Activators / classification; Trans-Activators / genetics
TL;DR: Comparison based homology modeling of the receiver domains of the OmpR sub‐family of response regulators in Bacillus subtilis and Escherichia coli suggests further sub‐classification is possible. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Naturally-occurring modification restricts the anticodon domain conformational space of tRNA(Phe)

JOURNAL OF MOLECULAR BIOLOGY, 334(5), 901–918.

By: J. Stuart n, K. Koshlap n, R. Guenther n & P. Agris n

author keywords: methylation; anticodon dynamics; tRNA position 37; codon recognition; frameshifting
MeSH headings : Anticodon; Base Sequence; Molecular Sequence Data; Nuclear Magnetic Resonance, Biomolecular; Nucleic Acid Conformation; RNA, Transfer, Phe / chemistry
TL;DR: A comparison of the resulting structures indicates that modification of position 37 affects the accuracy of decoding and the maintenance of the mRNA reading frame by restricting anticodon loop conformational space. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Conformational change in the 16S rRNA in the Escherichia coli 70S ribosome induced by P/P- and P/E-site tRNA(Phe) binding

BIOCHEMISTRY, 42(49), 14386–14396.

MeSH headings : Acetylation / radiation effects; Binding Sites / radiation effects; Cytosine / chemistry; Cytosine / radiation effects; Escherichia coli / chemistry; Escherichia coli / genetics; Escherichia coli / radiation effects; Nucleic Acid Conformation / radiation effects; Peptide Chain Elongation, Translational / genetics; Peptide Chain Elongation, Translational / radiation effects; Photochemistry; Poly U / chemistry; Poly U / genetics; RNA, Bacterial / chemistry; RNA, Bacterial / genetics; RNA, Bacterial / radiation effects; RNA, Messenger / chemistry; RNA, Messenger / genetics; RNA, Messenger / radiation effects; RNA, Ribosomal, 16S / chemistry; RNA, Ribosomal, 16S / genetics; RNA, Ribosomal, 16S / radiation effects; RNA, Transfer, Phe / chemistry; RNA, Transfer, Phe / genetics; RNA, Transfer, Phe / radiation effects; Ribosomes / chemistry; Ribosomes / genetics; Ribosomes / radiation effects; Transcription, Genetic / radiation effects; Ultraviolet Rays
TL;DR: Results indicate a limited structural change in the small subunit around C967 and C1400 during tRNA P-site binding sensitive to the type of mRNA that is used, and indicates an alternative arrangement in this region in empty ribosomes. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Compounds interacting with the ethylene receptor in plants

Plant Biology (Stuttgart, Germany), 5(5), 473–480.

By: E. Sisler & M. Serek

Source: NC State University Libraries
Added: August 6, 2018

2003 journal article

Characterisation of a pine MYB that regulates lignification

PLANT JOURNAL, 36(6), 743–754.

By: A. Patzlaff*, S. McInnis*, A. Courtenay*, C. Surman*, L. Newman*, C. Smith*, M. Bevan*, S. Mansfield* ...

author keywords: lignin; MYB; transcription; wood; pine; phenylpropanoid
MeSH headings : Amino Acid Sequence; Arabidopsis / genetics; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / genetics; Base Sequence; DNA, Complementary / genetics; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / genetics; Gene Expression Regulation, Plant / genetics; Gene Library; Genes, myb / genetics; Lignin / genetics; Molecular Sequence Data; Pinus / genetics; Plant Proteins / chemistry; Plant Proteins / genetics; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; RNA, Plant / genetics; RNA, Plant / isolation & purification; Sequence Alignment; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Tobacco / metabolism; Transcription Factors / chemistry; Transcription Factors / genetics
TL;DR: Electrophoretic mobility shift assays showed that recombinant PtMYB4 protein is able to bind to DNA motifs known as AC elements, consistent with the hypothesis that PtmyB4 is sufficient to induce lignification, and that it may play this role during wood formation in pine. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Using capillary electrophoresis to study methylation effect on RNA-peptide interaction

Acta Biochimica Polonica, 50(3), 857–864.

By: P. Mucha, A. Szyk, P. Rekowski & P. Agris

Source: NC State University Libraries
Added: August 6, 2018

2003 journal article

Serum lipoproteins in the diabetes control and complications trial/epidemiology of diabetes intervention and complications cohort - Associations with gender and glycemia

DIABETES CARE, 26(3), 810–818.

By: A. Jenkins*, T. Lyons*, D. Zheng*, J. Otvos n, D. Lackland*, D. McGee*, W. Garvey*, R. Klein*

MeSH headings : Adult; Blood Glucose; Cholesterol, HDL / blood; Cholesterol, LDL / blood; Cholesterol, VLDL / blood; Cohort Studies; Diabetes Mellitus, Type 1 / blood; Diabetes Mellitus, Type 1 / complications; Diabetes Mellitus, Type 1 / epidemiology; Female; Glycated Hemoglobin / analysis; Humans; Hyperglycemia / blood; Hyperglycemia / complications; Hyperglycemia / epidemiology; Lipoproteins / blood; Lipoproteins, LDL / metabolism; Magnetic Resonance Spectroscopy; Male; Middle Aged; Risk Factors; Sex Distribution; Triglycerides / blood
TL;DR: Male gender and poor glycemia are associated with a potentially more atherogenic NMR lipoprotein profile and LDL susceptibility to in vitro oxidation in type 1 diabetes. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

RpoS-dependent stress response and exoenzyme production in Vibrio vulnificus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 69(10), 6114–6120.

By: A. Hulsmann*, T. Rosche*, I. Kong*, H. Hassan n, D. Beam* & J. Oliver*

MeSH headings : Culture Media; Enzymes / biosynthesis; Heat-Shock Response; Humans; Hydrogen Peroxide / pharmacology; Hydrogen-Ion Concentration; Molecular Sequence Data; Movement; Mutation; Osmolar Concentration; Vibrio vulnificus / enzymology; Vibrio vulnificus / genetics; Vibrio vulnificus / growth & development; Vibrio vulnificus / physiology
TL;DR: These studies suggest that rpoS in V. vulnificus is important for adaptation to environmental changes and may have a role in virulence. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Molecular characterization and functional analysis of the manganese-containing superoxide dismutase gene (sodA) from Streptococcus thermophilus AO54

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 420(1), 103–113.

By: J. Andrus n, S. Bowen n, T. Klaenhammer n & H. Hassan n

author keywords: Streptococcus thermophilus; superoxide dismutase; MnSOD; sodA sequence; sodA mutant; SOD-null mutant; oxygen sensitivity; manganese ions; DNA polymerase III
MeSH headings : Amino Acid Sequence; Gene Expression Regulation, Enzymologic / drug effects; Magnesium Chloride / pharmacology; Molecular Sequence Data; Mutagenesis, Site-Directed; Sequence Alignment; Sequence Analysis, Protein; Species Specificity; Streptococcus / chemistry; Streptococcus / drug effects; Streptococcus / enzymology; Streptococcus / genetics; Structure-Activity Relationship; Superoxide Dismutase / chemistry; Superoxide Dismutase / genetics; Superoxide Dismutase / metabolism
TL;DR: The results suggest that sodA is essential for the aerobic growth of S. thermophilus and in the absence of functional SodA, manganese ions may provide partial protection against oxygen toxicity. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Efficient RNA 2 '-O-methylation requires juxtaposed and symmetrically assembled archaeal box C/D and C '/D ' RNPs

EMBO JOURNAL, 22(15), 3930–3940.

By: E. Tran n, . Zhang & E. Maxwell

author keywords: Archaea; box C; D RNP; ribonucleotide methylation; snoRNA; sRNA
MeSH headings : Archaea / metabolism; Archaeal Proteins / chemistry; Archaeal Proteins / metabolism; Base Sequence; Methylation; Molecular Sequence Data; Nucleic Acid Conformation; Protein Binding; RNA, Archaeal / chemistry; RNA, Archaeal / metabolism; Ribonucleoproteins / metabolism
TL;DR: In vitro assembly of an archaeal box C/D sRNP using recombinant core proteins L7, Nop56/58 and fibrillarin has yielded an RNA:protein enzyme that guides methylation from both the terminal box C-D core and internal C′/D′ RNP complexes. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Efficacy of new inhibitors of ethylene perception in improvement of display life of kalanchoe (Kalanchoe blossfeldiana Poelln.) flowers

Postharvest Biology and Technology, 30(2), 169–176.

By: Z. Kebenei, E. Sisler, T. Winkelmann & M. Serek

Source: NC State University Libraries
Added: August 6, 2018

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