Works Published in 2009
Displaying works 21 - 40 of 47 in total
Sorted by most recent date added to the index first, which may not be the same as publication date order.
2009 review
Sindbis virus as a model for studies of conformational changes in a metastable virus and the role of conformational changes in in vitro antibody neutralisation
[Review of ]. REVIEWS IN MEDICAL VIROLOGY, 19(5), 257–272.
MeSH headings : Alphavirus Infections / immunology; Animals; Antibodies, Viral / chemistry; Antibodies, Viral / immunology; Neutralization Tests; Protein Conformation; Sindbis Virus / immunology; Viral Proteins / chemistry; Viral Proteins / immunology; Virus Assembly
TL;DR:
A model is presented which could extend the mechanism of antibody induced conformational neutralisation to any metastable virus structure, and this review will focus on the structural aspects of in vitro antibody neutralisation of a metastable alphavirus, Sindbis virus.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2009 article
Overexpression of a directed mutant of 14-3-3 omega in Arabidopsis leaves affects phosphorylation and protein content of nitrate reductase
Oh, M.-H., Huber, J. L., Shen, W., Athwal, G. S., Wu, X., & Huber, S. C. (2009, July). BOTANY, Vol. 87, pp. 691–701.
author keywords: Arabidopsis thaliana; 14-3-3 protein; beta-glucosidase; glutathione S-transferase; two-dimensional difference gel electrophoresis; site-directed mutagenesis
TL;DR:
This work generated transgenic Arabidopsis plants constitutively overexpressing a directed mutant of 14-3-3 isoform ω that inhibits phosphorylated nitrate reductase (pNR) in a largely divalent-cation-independent manner in vitro.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
Hydrogen bonding and packing density are factors most strongly connected to limiting sites of high flexibility in the 16S rRNA in the 30S ribosome
BMC Structural Biology, 9.
Source: NC State University Libraries
Added: August 6, 2018
2009 journal article
Characterization of Conserved and Nonconserved Imprinted Genes in Swine
BIOLOGY OF REPRODUCTION, 81(5), 906–920.
author keywords: assisted reproductive technology; comparative genomic imprinting; epigenetics; gene regulation; genomic imprinting; parthenogenesis; placenta; swine parthenote
MeSH headings : Animals; Female; Fetus / anatomy & histology; Gene Expression Profiling; Gene Expression Regulation, Developmental; Genomic Imprinting / genetics; Oligonucleotide Array Sequence Analysis; Organ Size; Parthenogenesis / genetics; Placenta / anatomy & histology; Polymorphism, Single Nucleotide; Pregnancy; Reverse Transcriptase Polymerase Chain Reaction; Staining and Labeling; Swine / genetics
TL;DR:
To increase the understanding of imprinted genes in swine, a comprehensive analysis of this gene family was carried out using two complementary approaches: expression and phenotypic profiling of parthenogenetic fetuses, and analysis of imprinting by pyrosequencing.
(via Semantic Scholar)
academic.oup.com (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
Analysis of potato virus X replicase and TGBp3 subcellular locations
VIROLOGY, 393(2), 272–285.
author keywords: Potexvirus; Potato virus X; Triple gene block; Replicase
MeSH headings : Cell Membrane / virology; Cerulenin / pharmacology; Endoplasmic Reticulum / metabolism; Endoplasmic Reticulum / virology; Green Fluorescent Proteins / genetics; Green Fluorescent Proteins / metabolism; Microscopy, Confocal; Microscopy, Electron, Transmission; Plant Leaves / ultrastructure; Plant Leaves / virology; Potexvirus / genetics; Potexvirus / metabolism; Protoplasts / virology; RNA-Dependent RNA Polymerase / genetics; RNA-Dependent RNA Polymerase / metabolism; Tobacco / virology; Viral Proteins / genetics; Viral Proteins / metabolism
TL;DR:
The combined data indicate that PVX replication relies on ER-derived membrane recruitment and membrane proliferation, and there is no evidence to indicate that either PVX protein moves into the Golgi apparatus.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
A role for hydrophobicity in a DielsAlder reaction catalyzed by pyridyl-modified RNA
NUCLEIC ACIDS RESEARCH, 37(9), 3074–3082.
MeSH headings : Anthracenes / metabolism; Base Sequence; Catalysis; Ethylene Glycols / metabolism; Hydrophobic and Hydrophilic Interactions; Lactones; Molecular Sequence Data; Nucleic Acid Conformation; Pyridines / chemistry; RNA / chemistry; RNA / metabolism; RNA, Catalytic / chemistry; RNA, Catalytic / metabolism; Sesquiterpenes
TL;DR:
It is shown that DA_TR96, the most active member of the DA22 RNA sequence family, which was selected with pyridyl-modified nucleotides, accelerates a cycloaddition reaction between anthracene and maleimide derivatives with high turnover, and suggests a key role for the hydrophobic effect in the catalytic mechanism.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
A high capacity Alphavirus heterologous gene delivery system
VIROLOGY, 390(2), 368–373.
author keywords: Alphavirus; Gene delivery; Morphological variants
MeSH headings : Animals; Cell Line; Cricetinae; Culicidae; Gene Transfer Techniques; Genetic Vectors; Microscopy, Electron, Transmission; Mutation, Missense; Sindbis Virus / genetics; Viral Proteins / genetics; Virion / ultrastructure
TL;DR:
The first generation prototype larger vehicle, described here, carries a approximately 18 kb cDNA insert, however it is conceivable that RNA as large as 32 kb could be transcribed and packaged, and the resulting cargo containing virus particles are infectious.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
Multiple solvent crystal structures of ribonuclease A: An assessment of the method
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 76(4), 861–881.
author keywords: organic solvents; solvent mapping; plasticity; hydration; protein binding sites
MeSH headings : Animals; Catalytic Domain; Cattle; Computer Simulation; Crystallography, X-Ray; Models, Molecular; Pancreas / enzymology; Protein Binding; Protein Conformation; Ribonuclease, Pancreatic / chemistry; Ribonuclease, Pancreatic / metabolism; Solvents / chemistry; Solvents / metabolism; Water / chemistry; Water / metabolism
TL;DR:
Comparison of the MSCS structures with inhibitor‐bound crystal structures of RNAse A reveals that the organic solvent molecules identify key interactions made by inhibitor molecules, highlighting ligand binding hot‐spots in the active site.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
Differential Incorporation of Cholesterol by Sindbis Virus Grown in Mammalian or Insect Cells
JOURNAL OF VIROLOGY, 83(18), 9113–9121.
MeSH headings : Alphavirus Infections / etiology; Animals; Cell Line; Cell Membrane / chemistry; Cholesterol / analysis; Cholesterol / deficiency; Cholesterol / metabolism; Insecta; Lipids / analysis; Mammals; Mass Spectrometry; Sindbis Virus / metabolism; Sindbis Virus / pathogenicity
TL;DR:
The cholesterol dependence reported for infectivity and assembly of Sindbis virus is a reflection of differences in the insect cell lines used and the methods of delipidation, and the amount of cholesterol in the host cell membrane in and of itself has no effect on the process of virus assembly or on the ability of virus to infect cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
12. Responsible Consumption and Production
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
A Dimeric Structure for Archaeal Box C/D Small Ribonucleoproteins
SCIENCE, 325(5946), 1384–1387.
MeSH headings : Archaeal Proteins / chemistry; Archaeal Proteins / metabolism; Archaeal Proteins / ultrastructure; Base Sequence; Chromosomal Proteins, Non-Histone / chemistry; Methanococcales / chemistry; Microscopy, Electron; Models, Molecular; Molecular Weight; Nucleic Acid Conformation; Protein Multimerization; Protein Structure, Quaternary; Protein Structure, Tertiary; RNA, Archaeal / chemistry; RNA, Archaeal / ultrastructure; Ribonucleoproteins / chemistry; Ribonucleoproteins / metabolism; Ribonucleoproteins / ultrastructure
TL;DR:
The structure of a reconstituted and catalytically active box C/D sRNP from the archaeon Methanocaldococcus jannaschii was determined by single-particle electron microscopy and Mutational analysis demonstrated that this di-sRNP structure was relevant for the enzymatic function of archaeal box C /D sRNPs.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
Structural and Motional Contributions of the Bacillus subtilis ClpC N-Domain to Adaptor Protein Interactions
JOURNAL OF MOLECULAR BIOLOGY, 387(3), 639–652.
author keywords: competence; adaptor protein proteosome interactions; HSP100/Clp N-domain; NMR dynamics
MeSH headings : Adaptor Proteins, Vesicular Transport / chemistry; Adaptor Proteins, Vesicular Transport / genetics; Adaptor Proteins, Vesicular Transport / metabolism; Amino Acid Sequence; Bacillus subtilis / genetics; Bacillus subtilis / metabolism; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Heat-Shock Proteins / chemistry; Heat-Shock Proteins / genetics; Heat-Shock Proteins / metabolism; Models, Molecular; Molecular Sequence Data; Nuclear Magnetic Resonance, Biomolecular; Protein Binding; Protein Structure, Secondary; Sequence Alignment; Static Electricity
TL;DR:
NMR experiments used to map the MecA adaptor protein interaction surface of N-ClpCR reveal that regions involved in the interaction possess conformational flexibility and conformational exchange on the microsecond-to-millisecond timescale.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
Comparison of the transcriptomes of American chestnut (Castanea dentata) and Chinese chestnut (Castanea mollissima) in response to the chestnut blight infection
BMC PLANT BIOLOGY, 9.
MeSH headings : Ascomycota; DNA, Complementary / genetics; Expressed Sequence Tags; Fagaceae / genetics; Fagaceae / microbiology; Gene Expression Profiling; Gene Expression Regulation, Plant; Gene Library; Genes, Plant; Genomics; Plant Diseases / genetics; RNA, Plant / genetics; Sequence Analysis, DNA
TL;DR:
This study sequenced the transcriptome from fungal infected and healthy stem tissues collected from blight-sensitive American chestnut and blight-resistant Chinese chestnut trees using ultra high throughput pyrosequencing to identify genes involved in resistance to Cryphonectria parasitica, resulting in the identification of a large set of cDNA unigenes.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
15. Life on Land
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
An Efficient Organic Solvent Based Extraction Method for the Proteomic Analysis of Arabidopsis Plasma Membranes
JOURNAL OF PROTEOME RESEARCH, 8(6), 2752–2767.
author keywords: Proteomics; mass spectrometry; liquid chromatography; strong cation exchanges; phase partitioning; membrane proteins; transporters; Arabidopsis; methanol
MeSH headings : Amino Acid Sequence; Arabidopsis / chemistry; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / isolation & purification; Arabidopsis Proteins / metabolism; Chemical Fractionation / methods; Chloroform; Chlorophyll; Chromatography, Ion Exchange; Databases, Protein; Membrane Proteins / chemistry; Membrane Proteins / isolation & purification; Membrane Proteins / metabolism; Methanol; Molecular Sequence Data; Peptide Fragments / chemistry; Peptide Fragments / metabolism; Protein Kinases / isolation & purification; Protein Kinases / metabolism; Proteomics / methods; Solvents / chemistry; Tandem Mass Spectrometry; Trypsin / metabolism
TL;DR:
The combined use of methanol and chloroform provides an effective method to study membrane proteins and can be readily applied to other tissues and cells types for proteomic analysis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2009 review
Practical approaches to protein folding and assembly: Spectroscopic strategies in thermodynamics and kinetics
[Review of ]. Methods in enzymology: biothermodynamics,vol 455, part a, 455, 1–39.
Source: NC State University Libraries
Added: August 6, 2018
2009 journal article
Mass Spectrometry Bioinformatics: Tools for Navigating the Proteomics Landscape
CURRENT ANALYTICAL CHEMISTRY, 5(2), 131–143.
author keywords: Mass spectrometry; Peptide identification; Product ion spectrum; Database searching; Proteomics; Bioinformatics
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2009 review
Genomics of growth traits in forest trees
[Review of ]. CURRENT OPINION IN PLANT BIOLOGY, 12(2), 148–156.
MeSH headings : Genomics; Plants, Genetically Modified; Population Dynamics; Quantitative Trait Loci / genetics; Quantitative Trait, Heritable; Trees / genetics; Trees / growth & development
TL;DR:
Genetics and genomics are unifying disciplines that will serve well to dissect the variables and mechanisms of tree growth and development.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
2009 review
Advances in Quantitative Mass Spectrometry Analysis: Weighing in on Isotope Coding and Label-Free Approaches for Expression and Functional Proteomics
[Review of ]. CURRENT ANALYTICAL CHEMISTRY, 5(2), 166–185.
author keywords: Mass spectrometry; Quantitative proteomics; Phosphoproteomics; Isotope coding; Label-free quantitation; Absolute quantitation
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
Fragment-based identification of druggable 'hot spots' of proteins using Fourier domain correlation techniques
BIOINFORMATICS, 25(5), 621–627.
MeSH headings : Algorithms; Binding Sites; Crystallography, X-Ray; Internet; Models, Molecular; Protein Conformation; Protein Interaction Mapping; Proteins / chemistry
TL;DR:
The FTMAP algorithm is developed that performs global search of the entire protein surface for regions that bind a number of small organic probe molecules and shows that the major CSs trace out the shape of the first approved renin inhibitor, aliskiren.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2009 journal article
EARLY FLORAL DEVELOPMENT OF HELICONIA LATISPATHA (HELICONIACEAE), A KEY TAXON FOR UNDERSTANDING THE EVOLUTION OF FLOWER DEVELOPMENT IN THE ZINGIBERALES
AMERICAN JOURNAL OF BOTANY, 96(3), 580–593.
author keywords: floral development; floral evolution; Heliconia latispatha; heterochrony; morphology; nectaries; oblique zygomorphy; Zingiberales
TL;DR:
Here, epi-illumination light microscopy and reconstruction of serial sections are used to investigate the ontogeny of the Heliconia flower with emphasis on the staminode, and a comparison of floral structure and development across Zingiberales is presented to better understand the evolution of the flower in this charismatic group of tropical plants.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018
2009 review
Cancer and the tumor microenvironment: A review of an essential relationship
[Review of ]. Cancer Chemotherapy and Pharmacology, 63(4), 571–582.
Source: NC State University Libraries
Added: August 6, 2018
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