Molecular and Structural Biochemistry

MeSH headings : Apoptosis; Binding Sites; Calcium / chemistry; Caspase 3; Caspase 6; Caspase 7; Caspases / chemistry; Caspases / metabolism; Cations; Dimerization; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Fluorescence Resonance Energy Transfer; Humans; Hydrogen-Ion Concentration; Ions; Magnesium / chemistry; Models, Chemical; Molecular Chaperones / chemistry; Mutagenesis; Mutation; Peptides / chemistry; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Salts / chemistry; Salts / pharmacology; Temperature; Time Factors; Tryptophan / chemistry

TL;DR: The results show that caspase-3 is more sensitive to changes in pH and ion concentrations than is the zymogen and the prodomain acts as an intramolecular chaperone during assembly of the (pro)caspase subunits and increases the efficiency of formation of the native conformation. (via Semantic Scholar)

author keywords: assisted reproductive technology; anterior pituitary; follicle-stimulating hormone; gene regulation; ovulation

MeSH headings : Animals; Cells, Cultured; Doxycycline / pharmacology; Female; Follicle Stimulating Hormone / blood; Follicle Stimulating Hormone / genetics; Follicle Stimulating Hormone, beta Subunit / genetics; Follicle Stimulating Hormone, beta Subunit / metabolism; Genetic Engineering / methods; Male; Mice; Mice, Transgenic; Ovary / drug effects; Ovary / physiology; Ovulation / drug effects; Ovulation / genetics; Ovulation / physiology; Ovulation Induction / methods; Superovulation / genetics

TL;DR: In summary, gene switch technology and normal FSH regulation were combined to effectively enhance ovulation in mice and the same strategy can be used with any genetic switch to increase ovulation (or any highly conserved physiology) in any mammal. (via Semantic Scholar)