Works Published in 2002
Displaying works 1 - 20 of 42 in total
Sorted by most recent date added to the index first, which may not be the same as publication date order.
2002 article
Sweetpotato Genomics at North Carolina State University
PROCEEDINGS OF THE FIRST INTERNATIONAL CONFERENCE ON SWEETPOTATO: FOOD AND HEALTH FOR THE FUTURE, pp. 51–60.
author keywords: Ipomoea batatas; molecular markers; sequencing; DNA library; database; gene expression
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
15. Life on Land
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2002 journal article
Loci for CETP, LPL, LIPC, and APOC3 affect plasma lipoprotein size and subpopulation distribution in Hispanic and non- Hispanic white subjects: the Columbia University BioMarkers Study
Nutrition, Metabolism, and Cardiovascular Diseases, 12(4), 163–172.
Source: NC State University Libraries
Added: August 6, 2018
2002 journal article
Relations of lipoprotein subclass levels and low-density lipoprotein size to progression of coronary artery disease in the pravastatin limitation of atherosclerosis in the coronary arteries (PLAC-I) trial
AMERICAN JOURNAL OF CARDIOLOGY, 90(2), 89–94.
MeSH headings : Anticholesteremic Agents / pharmacology; Cholesterol / blood; Coronary Angiography; Coronary Artery Disease / blood; Coronary Artery Disease / diagnostic imaging; Coronary Artery Disease / drug therapy; Coronary Vessels / drug effects; Disease Progression; Female; Humans; Lipoproteins / blood; Lipoproteins / classification; Lipoproteins / drug effects; Lipoproteins, LDL / blood; Lipoproteins, LDL / drug effects; Male; Middle Aged; Particle Size; Pravastatin / pharmacology; Treatment Outcome
TL;DR:
Results indicate that levels of various lipoprotein subclasses may provide useful information on CAD risk even if levels of traditional risk factors are known.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2002 journal article
Purification and characterization of the fusion protein trypsin-streptavidin expressed in Escherichia coli
JOURNAL OF PROTEIN CHEMISTRY, 21(6), 413–418.
author keywords: affinity column; fusion protein; protein expression; protein purification; trypsin; streptavidin
MeSH headings : Blotting, Western; Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Escherichia coli / genetics; Recombinant Fusion Proteins / chemistry; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / isolation & purification; Streptavidin / chemistry; Trypsin / chemistry
TL;DR:
The molecular size of the soluble purified fusion protein was determined by size-exclusion chromatography using Superose 12 FPLC, indicating that the soluble protein exists as a monomer; thus, the presence of the trypsin domain must prevent the streptavidin domain from tetramer formation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2002 journal article
Modification of the rheological properties of whey protein isolate through the use of an immobilized microbial transglutaminase
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 50(20), 5546–5551.
author keywords: immobilization; transglutaminase; viscosity; whey protein isolate; gelation; cross linking
MeSH headings : Adsorption; Avidin / chemistry; Bacteria / enzymology; Biotinylation; Cross-Linking Reagents; Enzymes, Immobilized; Glass; Microspheres; Milk Proteins / chemistry; Rheology; Solutions; Transglutaminases / metabolism; Viscosity; Whey Proteins
TL;DR:
The functional properties of several batches of WPI were modified using <10 mg of the same enzyme, illustrating the capacity of immobilized enzymes to be used more frequently in applications of this nature.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2002 journal article
Macromolecular assembly of the transition state regulator AbrB in its unbound and complexed states probed by microelectrospray ionization mass spectrometry
ANALYTICAL BIOCHEMISTRY, 306(2), 222–227.
author keywords: AbrB; transition-state regulator; macromolecular assembly; mass spectrometry
MeSH headings : Bacillus subtilis; Bacterial Proteins / chemistry; Bacterial Proteins / metabolism; Chromatography, Gel; DNA / metabolism; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / metabolism; Escherichia coli; Macromolecular Substances; Protein Binding; Recombinant Proteins / chemistry; Recombinant Proteins / metabolism; Spectrometry, Mass, Electrospray Ionization; Transcription Factors / chemistry; Transcription Factors / metabolism
TL;DR:
This study represents the first detailed characterization of the stoichiometry of a transition-state regulator binding to one of its target promoters and demonstrates a capacity for DNA binding, underscoring the role of the N-terminal domain in both DNA recognition and dimerization.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2002 journal article
Cellular protein binds to sequences near the 5 ' terminus of potato virus X RNA that are important for virus replication
VIROLOGY, 301(2), 305–312.
MeSH headings : 5' Untranslated Regions / chemistry; 5' Untranslated Regions / metabolism; 5' Untranslated Regions / physiology; Base Sequence; Binding Sites; Molecular Sequence Data; Mutagenesis, Site-Directed; Nucleic Acid Conformation; Plant Proteins / metabolism; Potexvirus / genetics; Potexvirus / physiology; Protein Binding; Protoplasts / metabolism; RNA, Viral / chemistry; RNA, Viral / metabolism; RNA, Viral / physiology; RNA-Binding Proteins / metabolism; Tobacco; Virus Replication
TL;DR:
Electrophoretic mobility shift assays with protoplast protein extracts and RNA sequences from the PVX 5'-NTR show that the 5' region of PVX positive-strand RNA formed complexes with cellular proteins, and suggest that the p54 may function in PVX RNA replication by binding to the5' terminus of the viral genomic RNA.
(via Semantic Scholar)
doi.org (publisher)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2002 journal article
Structural and catalytic response to temperature and cosolvents of carboxylesterase EST1 from the extremely thermoacidophilic archaeon Sulfolobus solfataricus P1
BIOTECHNOLOGY AND BIOENGINEERING, 80(7), 784–793.
author keywords: DMSO; conformational flexibility; enzyme activation; thermostable
MeSH headings : Acetonitriles / chemistry; Carboxylesterase; Carboxylic Ester Hydrolases / biosynthesis; Carboxylic Ester Hydrolases / chemistry; Carboxylic Ester Hydrolases / isolation & purification; Catalysis; Dimethyl Sulfoxide / chemistry; Dimethylformamide / chemistry; Dioxanes / chemistry; Enzyme Activation; Enzyme Stability; Molecular Conformation; Quality Control; Sensitivity and Specificity; Solvents / chemistry; Species Specificity; Structure-Activity Relationship; Sulfolobus / classification; Sulfolobus / enzymology; Temperature
TL;DR:
Results suggest cosolvents may also serve as activators in applications involving thermostable biocatalysts at sub-optimal temperatures, related to small changes in the enzyme's structure resulting in an increase in its conformational flexibility.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
12. Responsible Consumption and Production
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2002 journal article
Comparison of X-ray detectors for a diffraction enhanced imaging system
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT, 491(1-2), 280–290.
author keywords: digital detector; image plate; CCD; point spread function; synchrotron; modulation transfer function; diffraction enhanced imaging
TL;DR:
While all three systems had comparable image quality, the CCDs had faster readout time than the image plate system and the Fuji system had the largest dynamic range and the largest active area.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2002 journal article
Adsorptive immobilization of cytochrome c on indium/tin oxide (ITO): electrochemical evidence for electron transfer-induced conformational changes
ELECTROCHEMISTRY COMMUNICATIONS, 4(2), 177–181.
author keywords: cytochrome c monolayers; indium/tin oxide; redox-linked conformational changes; interfacial electron transfer kinetics; unusual quasireversibility
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2002 journal article
Trypsin imobilization on derivatized cellulose beads by biospecific avidin-biotin interaction and characterization of the immobilized activity
Journal of Food Biochemistry, 26(2), 119–129.
TL;DR:
It is indicated that cellulose beads can be biotinylated for use as bioselective support for trypsin bioreactors and avidin-biotin interaction.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018
2002 journal article
Current approaches for the study of large proteins by NMR
JOURNAL OF MOLECULAR STRUCTURE, 602, 275–292.
author keywords: NMR; large proteins; structure; methods
TL;DR:
An overview of current methods employed for characterizing larger (>25 kDa) proteins by NMR is presented and the attenuation of T 2 relaxation effects by offsetting dipole–dipole and chemical shift anisotropy relaxation mechanisms is presented.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2002 journal article
Two E2F elements regulate the proliferating cell nuclear antigen promoter differently during leaf development
PLANT CELL, 14(12), 3225–3236.
MeSH headings : Base Sequence; Binding Sites / genetics; Cell Cycle Proteins; DNA-Binding Proteins; E2F Transcription Factors; E2F1 Transcription Factor; Geminiviridae / genetics; Geminiviridae / physiology; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Molecular Sequence Data; Mutation; Plant Leaves / genetics; Plant Leaves / growth & development; Plant Leaves / virology; Plants, Genetically Modified; Proliferating Cell Nuclear Antigen / genetics; Proliferating Cell Nuclear Antigen / metabolism; Promoter Regions, Genetic / genetics; Protein Binding; Response Elements / genetics; Tobacco / genetics; Tobacco / growth & development; Tobacco / virology; Transcription Factors / genetics; Transcription Factors / metabolism
TL;DR:
Examination of the distal element (E2F1) and how it interacts with the E2F2 site to regulate the PCNA promoter demonstrates that both E2f elements contribute to the repression of thePCNA promoter in mature leaves, whereas the E 2F1 site counters the repression activity of the E1F2 element in young leaves.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2002 journal article
Host DNA replication is induced by geminivirus infection of differentiated plant cells
PLANT CELL, 14(12), 2995–3007.
MeSH headings : Aphidicolin / pharmacology; Bromodeoxyuridine / metabolism; Cell Differentiation / genetics; Cell Differentiation / physiology; DNA Replication / drug effects; DNA Replication / genetics; DNA Replication / physiology; DNA, Viral / drug effects; DNA, Viral / metabolism; Geminiviridae / genetics; Geminiviridae / physiology; Hydroxyurea / pharmacology; Immunohistochemistry; In Situ Hybridization; Microscopy, Fluorescence; Tobacco / chemistry; Tobacco / genetics; Tobacco / virology
TL;DR:
5-bromo-2-deoxyuridine incorporation to examine DNA synthesis directly in infected Nicotiana benthamiana plants to determine if viral reprogramming of host replication controls had an impact on host DNA replication and demonstrate that chromosomal DNA is replicated in TGMV-infected cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2002 journal article
Highly conserved modified nucleosides influence Mg2+-dependent tRNA folding
NUCLEIC ACIDS RESEARCH, 30(21), 4751–4760.
MeSH headings : Base Sequence; Electrophoretic Mobility Shift Assay; Hydrogen Bonding; Lead / pharmacology; Magnesium / pharmacology; Models, Molecular; Nucleic Acid Conformation / drug effects; Nucleosides / chemistry; Nucleosides / metabolism; RNA Stability / drug effects; RNA, Fungal / chemistry; RNA, Fungal / genetics; RNA, Fungal / metabolism; RNA, Transfer, Phe / chemistry; RNA, Transfer, Phe / genetics; RNA, Transfer, Phe / metabolism; Ribonuclease T1 / metabolism; Thermodynamics
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2002 journal article
Heterologous array analysis in Pinaceae: hybridization of Pinus taeda cDNA arrays with cDNA from needles and embryogenic cultures of P-taeda, P-sylvestris or Picea abies
COMPARATIVE AND FUNCTIONAL GENOMICS, 3(4), 306–318.
author keywords: cDNA array; conifer; normalization; functional genomics; pine; spruce
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2002 journal article
Diffraction enhanced imaging contrast mechanisms in breast cancer specimens
MEDICAL PHYSICS, 29(10), 2216–2221.
author keywords: radiography; diffraction enhanced imaging; breast cancer; x-ray; contrast mechanisms
MeSH headings : Absorption; Algorithms; Biophysical Phenomena; Biophysics; Breast Neoplasms / diagnostic imaging; Breast Neoplasms / pathology; Carcinoma, Lobular / diagnostic imaging; Carcinoma, Lobular / pathology; Contrast Media / pharmacology; Female; Humans; Models, Statistical; Radiography / methods; Scattering, Radiation; Synchrotrons
TL;DR:
The density difference of fibrils in breast cancer as measured by absorption images correlate well with the density difference derived from refraction angle images of DEI, and theDEI apparent absorption image and the image obtained with the DEI system at the top of the reflectivity curve have much greater contrast than that of the normal radiograph.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2002 journal article
Alternative splicing of a beta(4) subunit proline-rich motif regulates voltage-dependent gating and toxin block of Ca(v)2.1 Ca2+ channels
Journal of Neuroscience, 22(21), 9331–9339.
MeSH headings : Alternative Splicing / physiology; Amino Acid Motifs / physiology; Animals; Brain / metabolism; Calcium Channel Blockers / pharmacology; Calcium Channels, N-Type / drug effects; Calcium Channels, N-Type / genetics; Calcium Channels, N-Type / metabolism; Humans; Ion Channel Gating / drug effects; Ion Channel Gating / physiology; Membrane Potentials / physiology; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Nerve Tissue Proteins / genetics; Oocytes / metabolism; Organ Specificity; Patch-Clamp Techniques; Protein Structure, Tertiary / physiology; Protein Subunits / drug effects; Protein Subunits / genetics; Protein Subunits / metabolism; RNA, Messenger / metabolism; Sequence Deletion; Sequence Homology, Amino Acid; Spinal Cord / metabolism; Structure-Activity Relationship; Xenopus laevis; omega-Conotoxins / pharmacology
TL;DR:
The models reveal that alternative splicing of the β4 N terminus results in dramatic differences in surface charge distribution and localize the proline-rich motif of β4b to an extended arm structure that flanks what would be the equivalent of a highly modified PSD-95 carboxylate binding loop.
(via Semantic Scholar)
Source: NC State University Libraries
Added: August 6, 2018
2002 journal article
Isolation and partial characterization of CD36 from skim milk
JOURNAL OF DAIRY SCIENCE, 85(8), 1903–1908.
author keywords: CD36; milk fat globule membrane; skim milk; sulfhydryl oxidase
MeSH headings : Amino Acid Sequence; Animals; Blotting, Western; CD36 Antigens; Cattle; Centrifugation; Chromatography, Gel; Electrophoresis, Polyacrylamide Gel; Glycolipids / analysis; Glycoproteins / analysis; Glycosylation; Lipid Droplets; Membrane Glycoproteins / chemistry; Membrane Glycoproteins / isolation & purification; Milk / chemistry; Molecular Sequence Data; Molecular Weight; Organic Anion Transporters / chemistry; Organic Anion Transporters / isolation & purification; Ultrafiltration
TL;DR:
CD36, a common milk fat globule membrane glycoprotein, was isolated from skim milk by methods similar to those previously utilized for the isolation of sulfhydryl oxidase, giving similar purity as observed by electrophoresis and suggested tissue-specific glycosylation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2002 journal article
Interaction of RNA with phage display selected peptides analyzed by capillary electrophoresis mobility shift assay
RNA, 8(5), 698–704.
author keywords: anticodon stem and loop; capillary electrophoresis; methylated nucleoside-dependent RNA-peptide; interaction; phage display selected peptides; tRNA
MeSH headings : Amino Acid Sequence; Anticodon / chemistry; Anticodon / genetics; Anticodon / metabolism; Base Sequence; Electrophoresis, Capillary / methods; In Vitro Techniques; Molecular Sequence Data; Nucleic Acid Conformation; Peptide Library; Peptides / chemistry; Peptides / metabolism; RNA, Transfer, Phe / chemistry; RNA, Transfer, Phe / genetics; RNA, Transfer, Phe / metabolism
TL;DR:
Capillary electrophoresis was found to be an efficient and sensitive method for the qualitative analysis of RNA/peptide interaction and should be generally applicable to the study of RNA- peptide (protein) interactions.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
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