Works Published in 2006
Displaying works 1 - 20 of 54 in total
Sorted by most recent date added to the index first, which may not be the same as publication date order.
2006 journal article
A colorimetric assay for viral agents that produce cytopathic effects
Journal of Virological Methods, 135(1), 56–65.
author keywords: MTT; TCID50; plaque assay; parvovirus; Sindbis virus
MeSH headings : Animals; Cell Line; Colorimetry / methods; Cricetinae; Cytopathogenic Effect, Viral; Dogs; Formazans / metabolism; Humans; Minute Virus of Mice / pathogenicity; Parvovirus, Canine / pathogenicity; Parvovirus, Porcine / pathogenicity; Reproducibility of Results; Sindbis Virus / pathogenicity; Swine; Tetrazolium Salts / metabolism; Viral Plaque Assay; Viruses / pathogenicity
TL;DR:
MTT has been applied to measure cytopathic effect produced by viral infection for different members of the parvovirus family and an unrelated virus, Sindbis, which is routinely titrated by plaque assay.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Crossref
Added: October 9, 2021
2006 journal article
Uncoupling the Enzymatic and Autoprocessing Activities of Helicobacter pylori γ-Glutamyltranspeptidase
Journal of Biological Chemistry, 281(28), 19029–19037.
MeSH headings : Bacterial Proteins / chemistry; Biochemistry / methods; Helicobacter pylori / enzymology; Hydrogen-Ion Concentration; Hydrolysis; Kinetics; Peptides / chemistry; Protein Structure, Tertiary; Recombinant Proteins / chemistry; Threonine / chemistry; Time Factors; gamma-Glutamyltransferase / chemistry
TL;DR:
The specific contributions of a conserved threonine residue (Thr380) to autoprocessing and hydrolase activities were examined by mutagenesis using both the standard and coexpression systems and indicate that the γ-methyl group of Thr380 orients the hydroxyl group of this conserved residue, which is required for both the processing and hydrology reactions.
(via Semantic Scholar)
www.jbc.org (publisher PDF)
Source: Crossref
Added: October 29, 2020
2006 journal article
Structure, binding interface and hydrophobic transitions of Ca2+-loaded calbindin-D28K
Nature Structural & Molecular Biology, 13(7), 641–647.
MeSH headings : Amino Acid Sequence; Animals; Binding Sites; Calbindins; Calcium / metabolism; Cloning, Molecular; Hydrogen Bonding; Magnetic Resonance Spectroscopy; Models, Molecular; Peptides / chemistry; Protein Folding; Protein Structure, Secondary; Rats; Recombinant Proteins / chemistry; Recombinant Proteins / metabolism; S100 Calcium Binding Protein G / chemistry; S100 Calcium Binding Protein G / metabolism
TL;DR:
The structure, binding interface and hydrophobic characteristics of Ca2+-loaded calbindin-D28K provide the first detailed insights into how this essential protein may function.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Crossref
Added: August 28, 2020
2006 journal article
A preliminary neutron diffraction study of rasburicase, a recombinant urate oxidase enzyme, complexed with 8-azaxanthin.
Structural Biology and Crystallization Communications, 62(Pt 3), 306–309,
MeSH headings : Binding Sites; Crystallization / methods; Deuterium Oxide; Neutron Diffraction / methods; Recombinant Proteins / chemistry; Saccharomyces cerevisiae / metabolism; Urate Oxidase / chemistry; Xanthines / chemistry
TL;DR:
The high quality of the neutron diffraction data collected are suitable for high-resolution structural analysis of rasburicase, which has one of the largest primitive unit-cell volumes and molecular weights so far successfully studied with neutrons.
(via Semantic Scholar)
Source: ORCID
Added: January 6, 2020
2006 journal article
Comparison of hydrogen determination with X-ray and neutron crystallography in a human aldose reductase-inhibitor complex.
European Biophysics Journal : EBJ, 35(7), 577–583,
MeSH headings : Aldehyde Reductase / antagonists & inhibitors; Aldehyde Reductase / chemistry; Aldehyde Reductase / metabolism; Binding Sites; Crystallization; Crystallography, X-Ray; Deuterium / chemistry; Humans; Hydrogen / chemistry; Hydrogen Bonding; Models, Molecular; Molecular Structure; NADP / metabolism; Neutron Diffraction; Protein Conformation; Protons
TL;DR:
Both studies enable the determination of protonation states, with a clear advantage for neutrons in the case of less-ordered atoms (B > 5 Å2).
(via Semantic Scholar)
Source: ORCID
Added: January 6, 2020
2006 journal article
A quasi-Laue neutron crystallographic study of d-xylose isomerase
European Biophysics Journal, 35(7), 601–609.
MeSH headings : Aldose-Ketose Isomerases / chemistry; Aldose-Ketose Isomerases / metabolism; Binding Sites; Catalysis; Crystallography, X-Ray; Hydrogen / chemistry; Models, Chemical; Neutron Diffraction; Neutrons; Protein Binding; Streptomyces / enzymology; Xylose / metabolism
TL;DR:
The neutron structure shows unambiguously that residue His 53 is doubly protonated at the active site of d-xylose isomerase, which suggests that the reaction proceeds through an acid catalyzed opening of the sugar ring, which is in accord with the mechanism suggested by Fenn et al.
(via Semantic Scholar)
Source: ORCID
Added: January 6, 2020
2006 journal article
Neutron Laue macromolecular crystallography
European Biophysics Journal, 35(7), 611–620.
MeSH headings : Crystallography / trends; Deuterium / chemistry; Forecasting; Hydrogen Bonding; Macromolecular Substances / chemistry; Models, Molecular; Neutron Diffraction; Neutrons
TL;DR:
Improvements in modern molecular biology now allow fully (per)deuterated protein samples to be produced for neutron scattering that essentially eradicate the large—and ultimately limiting—hydrogen incoherent scattering background that has hampered such studies in the past.
(via Semantic Scholar)
Source: ORCID
Added: January 6, 2020
2006 article
MARCKS related peptide improves airway obstruction related to mucus secretion in a mouse model of asthma.
404nOtfound, Vol. 3, p. A713.
Source: NC State University Libraries
Added: August 6, 2018
2006 patent
Method to inhibit ethylene responses in plants
Washington, DC: U.S. Patent and Trademark Office.
Source: NC State University Libraries
Added: August 6, 2018
2006 journal article
Geminivirus infection up-regulates the expression of two Arabidopsis protein kinases related to yeast SNF1-and mammalian AMPK-activating kinases
PLANT PHYSIOLOGY, 142(4), 1642–1655.
MeSH headings : AMP-Activated Protein Kinase Kinases; Amino Acid Sequence; Animals; Arabidopsis / enzymology; Arabidopsis / genetics; Arabidopsis / virology; Arabidopsis Proteins / chemistry; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Flowers / genetics; Flowers / metabolism; Flowers / virology; Fungal Proteins / chemistry; Geminiviridae / physiology; Gene Expression Regulation, Plant; Genetic Complementation Test; Molecular Sequence Data; Phylogeny; Plant Diseases / virology; Plant Leaves / genetics; Plant Leaves / metabolism; Plant Leaves / virology; Plant Shoots / genetics; Plant Shoots / metabolism; Plant Shoots / virology; Protein Kinases / chemistry; Protein Serine-Threonine Kinases / chemistry; Protein Serine-Threonine Kinases / genetics; Protein Serine-Threonine Kinases / metabolism; RNA, Messenger / metabolism; Sequence Alignment; Two-Hybrid System Techniques; Up-Regulation; Viral Proteins / metabolism
TL;DR:
Both full-length GRIK1 and GRIk2 interact with AL1 in yeast two-hybrid studies, suggesting that the Arabidopsis kinases mediate one or more processes during early plant development and geminivirus infection by activating SNF1-related kinases.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2006 journal article
Collision-induced dissociative chemical cross-linking reagents and methodology: Applications to protein structural characterization using tandem mass spectrometry analysis
ANALYTICAL CHEMISTRY, 78(23), 8059–8068.
MeSH headings : Amino Acid Sequence; Animals; Aspartic Acid / chemistry; Cross-Linking Reagents / chemistry; Glutathione Transferase / chemistry; Glutathione Transferase / genetics; Glutathione Transferase / metabolism; Models, Molecular; Molecular Sequence Data; Proline / chemistry; Protein Structure, Tertiary; Recombinant Fusion Proteins / chemistry; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Schistosoma japonicum / genetics; Schistosoma japonicum / metabolism; Serum Albumin, Bovine / chemistry; Serum Albumin, Bovine / genetics; Serum Albumin, Bovine / metabolism; Tandem Mass Spectrometry / methods
TL;DR:
Common acquisition approaches such as data-dependent acquisition experiments using ion trap mass spectrometers and product ion spectral analysis using SEQUEST were shown to be compatible with the CID-CXL-MS/MS reagents, obviating the requirement for high resolution and high mass accuracy measurements to identify both intra- and interpeptide cross-links.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2006 journal article
Oral hydration, parotid salivation and the perceived pleasantness of small water volumes
PHYSIOLOGY & BEHAVIOR, 89(5), 724–734.
author keywords: mouth dryness; water temperature; water pleasantness; salivation
MeSH headings : Adult; Analysis of Variance; Dose-Response Relationship, Drug; Euphoria / drug effects; Female; Humans; Male; Mouth / drug effects; Mouth / physiology; Parotid Gland / metabolism; Salivation / drug effects; Salivation / physiology; Taste / physiology; Temperature; Water / pharmacology
TL;DR:
A first experiment found that although the rate of parotid salivation was increased if the mouth had been dried using a warm airflow, the different water temperatures did not induce different rates of parotsied salivation, indicating that enhanced preference for cold water when the mouth is dry is not invariably based in the reward gained from mouth rewetting via increased parotsid saliva flow.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
2006 journal article
Detection of transcriptional difference of porcine imprinted genes using different microarray platforms
BMC Genomics, 7.
Source: NC State University Libraries
Added: August 6, 2018
2006 journal article
tRNA regulation of gene expression: Interactions of an mRNA 5 '-UTR with a regulatory tRNA
RNA, 12(7), 1254–1261.
author keywords: gene regulation; Gram-positive bacteria; tRNA; RNA experimental model
MeSH headings : 5' Untranslated Regions / genetics; Bacillus subtilis / genetics; Base Sequence; Electrophoresis, Polyacrylamide Gel; Gene Expression Regulation; Models, Molecular; Nucleic Acid Conformation; RNA, Bacterial / chemistry; RNA, Bacterial / genetics; RNA, Messenger / chemistry; RNA, Messenger / genetics; RNA, Transfer / chemistry; RNA, Transfer / genetics; RNA, Transfer / metabolism; Spectrometry, Fluorescence
TL;DR:
The bimolecular 5'-UTR and ASL(Gly)(GCC) models mimic the RNA-RNA interaction required for T box gene regulation in vivo.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2006 journal article
Transcriptional and functional analysis of oxalyl-coenzyme A (CoA) decarboxylase and formyl-CoA transferase genes from Lactobacillus acidophilus
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72(3), 1891–1899.
MeSH headings : Carboxy-Lyases / genetics; Carboxy-Lyases / metabolism; Coenzyme A-Transferases / genetics; Coenzyme A-Transferases / metabolism; Gene Expression Regulation, Bacterial; Lactobacillus acidophilus / enzymology; Lactobacillus acidophilus / genetics; Lactobacillus acidophilus / growth & development; Oligonucleotide Array Sequence Analysis; Operon; Oxalates / metabolism; Reverse Transcriptase Polymerase Chain Reaction; Transcription, Genetic
TL;DR:
Transcriptional analysis using cDNA microarrays and reverse transcription-quantitative PCR revealed that mildly acidic conditions were a prerequisite for frc and oxc transcription, andOxalate-dependent induction of these genes occurred only in cells first adapted to subinhibitory concentrations of oxalate and then exposed to pH 5.5.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
2006 review
The discovery and development of compounds counteracting ethylene at the receptor level
[Review of ]. BIOTECHNOLOGY ADVANCES, 24(4), 357–367.
author keywords: ethylene; ethylene agonist; ethylene antagonist; receptor; 1-methylcyclopropene (1-MCP)
MeSH headings : Cyclopropanes / pharmacology; Ethylenes / antagonists & inhibitors; Ethylenes / pharmacology; Flowers / cytology; Flowers / drug effects; Flowers / growth & development; Genes, Plant; Plant Growth Regulators / pharmacology; Plants, Genetically Modified; Receptors, Cell Surface / drug effects; Receptors, Cell Surface / genetics
TL;DR:
The discovery and development of 1-methylcyclopropene (1-MCP) was not done as a single step but rather was found by studying various aspects of ethylene action and the ethylene receptors.
(via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018
2006 journal article
Role of loop bundle hydrogen bonds in the maturation and activity of (pro) caspase-3
BIOCHEMISTRY, 45(44), 13249–13263.
MeSH headings : Amino Acid Sequence; Base Sequence; Caspase 3 / chemistry; Caspase 3 / metabolism; Crystallography; DNA Primers; Hydrogen Bonding; Hydrogen-Ion Concentration; Hydrolysis; Models, Molecular; Molecular Sequence Data; Protein Conformation; Spectrometry, Fluorescence
TL;DR:
It is shown that replacing the residues affects the activity of the procaspase as well as the mature caspase, with D169A and E167A replacements having the largest effects.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2006 journal article
Role of a conserved tripeptide in the endodomain of Sindbis virus glycoprotein E2 in virus assembly and function
JOURNAL OF GENERAL VIROLOGY, 87, 657–664.
MeSH headings : Acids; Amino Acid Sequence; Animals; Cell Fusion; Cell Line; Cricetinae; Molecular Sequence Data; Protein Structure, Tertiary / genetics; Protein Structure, Tertiary / physiology; Sindbis Virus / pathogenicity; Sindbis Virus / physiology; Viral Envelope Proteins / chemistry; Viral Envelope Proteins / genetics; Viral Envelope Proteins / physiology; Virulence; Virus Assembly
TL;DR:
The loss of infectivity in the double mutant is accompanied by the loss of the ability to fuse cells after brief exposure to acid pH, which supports the idea that these residues are vital for production of infectious/functional virus; however, they are dispensable for assembly.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2006 journal article
Polymerization of styrene in gamma-cyclodextrin channels: Lightly rotaxanated polystyrenes with altered stereosequences
POLYMER, 47(20), 6948–6955.
author keywords: cyclodextrin; inclusion compound; polystyrene
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2006 journal article
Peptide aptamers that bind to a geminivirus replication protein interfere with viral replication in plant cells
JOURNAL OF VIROLOGY, 80(12), 5841–5853.
MeSH headings : Aptamers, Peptide / pharmacology; DNA, Viral / analysis; Geminiviridae / chemistry; Geminiviridae / drug effects; Geminiviridae / physiology; Peptide Library; Plant Cells; Plant Viruses / drug effects; Plants / virology; Plants, Genetically Modified; Thioredoxins / genetics; Thioredoxins / metabolism; Transfection; Two-Hybrid System Techniques; Viral Proteins / antagonists & inhibitors; Viral Proteins / genetics; Viral Proteins / metabolism; Viral Proteins / physiology; Virus Replication / drug effects
TL;DR:
The peptide aptamers characterized in these studies represent new tools for studying AL1 function and can serve as the basis for the development of crops with broad-based resistance to single-stranded DNA viruses.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
3. Good Health and Well-being
(OpenAlex)
13. Climate Action
(Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018
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