TY - JOUR TI - The Role of the Technician in Poison Control and Treatment AU - Nettifee, J.A. T2 - Veterinary Technician DA - 1987/11// PY - 1987/11// VL - 8 IS - 10 SP - 481–486 ER - TY - JOUR TI - Inhibition of 12-O-Tetradecanoylphorbol-13-acetate Induction of Ornithine Decarboxylase Activity, DNA Synthesis, and Tumor Promotion in Mouse Skin by Ascorbic Acid and Ascorbyl Palmitate AU - Smart, R.C. AU - Huang, M.-T. AU - Han, Z.T. AU - Kaplan, M.C. AU - Focella, A. AU - Conney, A.H. T2 - Cancer Research DA - 1987/// PY - 1987/// VL - 47 IS - 24, Part 1 SP - 6633–6638 ER - TY - JOUR TI - Characterization of purified DT-diaphorase from liver cytosol of 3-methylcholanthrene-treated rats AU - Huang, M.-T. AU - Smart, R.C. AU - Thomas, P.E. AU - Pickett, C.B. AU - Lu, Y.H. T2 - Chemica Scripta DA - 1987/// PY - 1987/// VL - 27A:49-54 ER - TY - JOUR TI - Evaluation of medium- and long-chain triglycerides as energy sources in newborn pigs AU - Odle, J. AU - Benevenga, N.J. AU - Crenshaw, T.D. T2 - Journal of Animal Science DA - 1987/// PY - 1987/// VL - 65 IS - Suppl. 1 SP - 307-308 ER - TY - RPRT TI - Energy for baby pig survival AU - Odle, J. AU - Benevenga, N.J. A3 - University of Wisconsin-Madison DA - 1987/// PY - 1987/// SP - 6–12 PB - University of Wisconsin-Madison ER - TY - JOUR TI - Ascorbic Acid, Alcohol, and Environmental Chemicals AU - Zannoni, V. G. AU - Brodfuehrer, J. I. AU - Smart, R. C. AU - Susick, R. L. T2 - Annals of the New York Academy of Sciences AB - Annals of the New York Academy of SciencesVolume 498, Issue 1 p. 364-388 Ascorbic Acid, Alcohol, and Environmental Chemicalsa V. G. ZANNONI, V. G. ZANNONI Department of Pharmacology University of Michigan Medical School Ann Arbor, Michigan 48109Search for more papers by this authorJ. I. BRODFUEHRER, J. I. BRODFUEHRER Department of Pharmacology University of Michigan Medical School Ann Arbor, Michigan 48109Search for more papers by this authorR. C. SMART, R. C. SMART Department of Pharmacology University of Michigan Medical School Ann Arbor, Michigan 48109 Toxicology Program, North Carolina State University, Raleigh, N.C. 27695.Search for more papers by this authorR. L. SUSICK JR., R. L. SUSICK JR. Department of Pharmacology University of Michigan Medical School Ann Arbor, Michigan 48109 Department of Pathology and Experimental Toxicology, Warner-Lambert/Parke-Davis, Ann Arbor, Mich. 48105.Search for more papers by this author V. G. ZANNONI, V. G. ZANNONI Department of Pharmacology University of Michigan Medical School Ann Arbor, Michigan 48109Search for more papers by this authorJ. I. BRODFUEHRER, J. I. BRODFUEHRER Department of Pharmacology University of Michigan Medical School Ann Arbor, Michigan 48109Search for more papers by this authorR. C. SMART, R. C. SMART Department of Pharmacology University of Michigan Medical School Ann Arbor, Michigan 48109 Toxicology Program, North Carolina State University, Raleigh, N.C. 27695.Search for more papers by this authorR. L. SUSICK JR., R. L. SUSICK JR. Department of Pharmacology University of Michigan Medical School Ann Arbor, Michigan 48109 Department of Pathology and Experimental Toxicology, Warner-Lambert/Parke-Davis, Ann Arbor, Mich. 48105.Search for more papers by this author First published: July 1987 https://doi.org/10.1111/j.1749-6632.1987.tb23775.xCitations: 2 a This work supported in part by grant no. 23007 from Hoffmann-La Roche, Inc., Nutley, NJ. and grant nos. 5M01-RR00042 and 2 P60 AM20572 from the National Institutes of Health. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume498, Issue1Third Conference on Vitamin CJuly 1987Pages 364-388 RelatedInformation DA - 1987/7// PY - 1987/7// DO - 10.1111/j.1749-6632.1987.tb23775.x VL - 498 IS - 1 Third Confere SP - 364-388 J2 - Ann NY Acad Sci LA - en OP - SN - 0077-8923 1749-6632 UR - http://dx.doi.org/10.1111/j.1749-6632.1987.tb23775.x DB - Crossref ER - TY - SOUND TI - Estuarine Monitoring for Ulcerative Mycosis AU - Levine, J.F. DA - 1987/// PY - 1987/// M3 - workshop ER - TY - RPRT TI - Ulcerative Disease Regional Database Master Tutorial AU - Levine, J.F. AU - Lee, L.C. A3 - Florida Department of Environmental Regulation DA - 1987/// PY - 1987/// M3 - Software PB - Florida Department of Environmental Regulation ER - TY - JOUR TI - Sulfur Reduction by the Extremely Thermophilic Archaebacterium Pyrodictium occultum AU - Parameswaran, A. K. AU - Provan, C. N. AU - Sturm, F. J. AU - Kelly, R. M. T2 - Applied and Environmental Microbiology AB - The relationship between growth and biological sulfur reduction for the extremely thermophilic archaebacterium Pyrodictium occultum was studied over a temperature range of 98 to 105°C. The addition of yeast extract (0.2 g/liter) to the medium was found to increase hydrogen sulfide production significantly, especially at higher temperatures. Sulfide production in uninoculated controls with and without yeast extract was noticeable but substantially below the levels observed in samples containing the microorganism. DA - 1987/7// PY - 1987/7// DO - 10.1128/aem.53.7.1690-1693.1987 VL - 53 IS - 7 SP - 1690-1693 J2 - Appl Environ Microbiol LA - en OP - SN - 0099-2240 1098-5336 UR - http://dx.doi.org/10.1128/aem.53.7.1690-1693.1987 DB - Crossref ER - TY - JOUR TI - Growth of Extremely Thermophilic Archaebacteria under Elevated Hyperbaric Conditions AU - Sturm, F. J. AU - Parameswaran, A. K. AU - Provan, C. N. AU - Kelly, R. M. T2 - Annals of the New York Academy of Sciences AB - Annals of the New York Academy of SciencesVolume 506, Issue 1 p. 51-66 Growth of Extremely Thermophilic Archaebacteria under Elevated Hyperbaric Conditionsa F. J. STURM, F. J. STURM Department of Chemical Engineering The Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this authorA. K. PARAMESWARAN, A. K. PARAMESWARAN Department of Chemical Engineering The Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this authorC. N. PROVAN, C. N. PROVAN Department of Chemical Engineering The Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this authorR. M. KELLY, R. M. KELLY Department of Chemical Engineering The Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this author F. J. STURM, F. J. STURM Department of Chemical Engineering The Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this authorA. K. PARAMESWARAN, A. K. PARAMESWARAN Department of Chemical Engineering The Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this authorC. N. PROVAN, C. N. PROVAN Department of Chemical Engineering The Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this authorR. M. KELLY, R. M. KELLY Department of Chemical Engineering The Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this author First published: November 1987 https://doi.org/10.1111/j.1749-6632.1987.tb23809.xCitations: 4 a This work was supported in part through grants from the National Science Foundation (Nos. CPE-8405640, CBT-8507399, and CBT-8513441). F. J. Sturm acknowledges the support of the United States Coast Guard. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat REFERENCES 1 Deming, J. W. 1986. The biotechnological future for newly-described, extremely thermophilic bacteria. Microb. Ecol. 12: 111. 10.1007/BF02153226 PubMedWeb of Science®Google Scholar 2 Belkin, S. & H. W. Jannasch. 1985. A new extremely thermophilic, sulfur-reducing heterotrophic marine bacterium. Arch. Microbiol. 141: 181–186. 10.1007/BF00408055 CASWeb of Science®Google Scholar 3 McFeters, G. A. & J. T. Ulrich. 1972. Effect of temperature on the respiration and cytochromes of an extreme thermophile. J. Bacteriol. 110: 777. CASPubMedWeb of Science®Google Scholar 4 Woese, C. R. & R. S. Wolfe. 1985. The Bacteria. Vol. VIII: xix–xxiii. Academic Press. New York . Google Scholar 5 Holzman, D. 1985. Ribosomal RNA: Where the action is. Mosaic 16 (no. 2): 32–39. Google Scholar 6 Stetter, K. O. & W. Zillig. 1985. Thermoplasma and the thermophilic sulfur-dependent archaebacteria. In The Bacteria. Vol. VIII. C. R. Woese & R. S. Wolfe, Eds.: 85–170. Academic Press. New York . Google Scholar 7 Sturm, F. J., S. A. Hurwitz, J. W. Deming & R. M. Kelly. 1986. Growth of the extreme thermophile Sulfolobus acidocaldarius in a hyperbaric helium bioreactor. Biotechnol. Bioeng. In press. Google Scholar 8 Brierley, J. A. 1966. Contribution of chemolithoautotrophic bacteria to the acid thermal waters of the Geyser springs group in Yellowstone National Park. Ph.D thesis. Montana State University. Bozeman , Montana . Google Scholar 9 Stetter, K. O., H. Konig & E. Stackebrandt. 1983. Pyrodictium gen, nov. A new genus of submarine disc-shaped sulfur-reducing archaebacteria growing optimally at 105 °C. Syst. Appl. Microbiol. 4: 535–551. 10.1016/S0723-2020(83)80011-3 CASPubMedWeb of Science®Google Scholar 10 Stetter, K. O. 1983. Ultrathin mycelia-forming organisms from submarine volcanic areas having an optimum growth temperature of 105 °C. Nature 300: 258. 10.1038/300258a0 Web of Science®Google Scholar 11 Hurwitz, S. A. 1985. The effects of temperature and pressure on the growth of the extreme thermophile Sulfolobus acidocaldarius. Masters thesis. The Johns Hopkins University. Baltimore , Maryland . Google Scholar 12 Sturm, F. J. 1986. Growth of extremely thermophilic archaebacteria in a hyperbaric helium bioreactor. Appendix B. Masters thesis. The Johns Hopkins University. Baltimore , Maryland . Google Scholar 13 Hobbie, J. E., R. J. Daley & S. Jasper. 1977. Use of nucleopore filters for counting bacteria by fluorescence microscopy. Appl. Environ. Microbiol. 33: 1225–1228. 10.1128/AEM.33.5.1225-1228.1977 CASPubMedWeb of Science®Google Scholar 14 Yeh, T. Y., J. R. Godshalk, G. J. Olson & R. M. Kelly. 1986. Use of epifluorescence microscopy for characterizing the activity of Thiobacillus ferrooxidans on iron pyrite. Biotechnol. Bioeng. In press. Google Scholar 15 Hungate, R. E. 1969. A rolltube method for cultivation of strict anaerobes. In Methods in Microbiology. Vol. 3B. J.R. Norris & D.W. Ribbons, Eds.: 117–132. Academic Press. New York . Google Scholar Citing Literature Volume506, Issue1Biochemical Engineering VNovember 1987Pages 51-66 ReferencesRelatedInformation DA - 1987/11// PY - 1987/11// DO - 10.1111/j.1749-6632.1987.tb23809.x VL - 506 IS - 1 Biochemical E SP - 51-66 J2 - Ann NY Acad Sci LA - en OP - SN - 0077-8923 1749-6632 UR - http://dx.doi.org/10.1111/j.1749-6632.1987.tb23809.x DB - Crossref ER - TY - JOUR TI - Effect of Culturing Conditions on the Production of Exotoxin A by Pseudomonas aeruginosa AU - Blumentals, Ilse I. AU - Skaja, Anne K. AU - Kelly, Robert M. AU - Clem, Thomas R. AU - Shiloach, Joseph T2 - Annals of the New York Academy of Sciences AB - Annals of the New York Academy of SciencesVolume 506, Issue 1 p. 663-668 Effect of Culturing Conditions on the Production of Exotoxin A by Pseudomonas aeruginosa ILSE I. BLUMENTALS, ILSE I. BLUMENTALS Department of Chemical Engineering The Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this authorANNE K. SKAJA, ANNE K. SKAJA Department of Chemical Engineering The Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this authorROBERT M. KELLY, ROBERT M. KELLY Department of Chemical Engineering The Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this authorTHOMAS R. CLEM, THOMAS R. CLEM Biomedical Engineering and Instrumentation Branch Division of Research Services National Institutes of Health Bethesda, Maryland 20892Search for more papers by this authorJOSEPH SHILOACH, Corresponding Author JOSEPH SHILOACH Biotechnology Unit National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health Bethesda, Maryland 20892Author to whom correspondence should be addressed.Search for more papers by this author ILSE I. BLUMENTALS, ILSE I. BLUMENTALS Department of Chemical Engineering The Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this authorANNE K. SKAJA, ANNE K. SKAJA Department of Chemical Engineering The Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this authorROBERT M. KELLY, ROBERT M. KELLY Department of Chemical Engineering The Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this authorTHOMAS R. CLEM, THOMAS R. CLEM Biomedical Engineering and Instrumentation Branch Division of Research Services National Institutes of Health Bethesda, Maryland 20892Search for more papers by this authorJOSEPH SHILOACH, Corresponding Author JOSEPH SHILOACH Biotechnology Unit National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health Bethesda, Maryland 20892Author to whom correspondence should be addressed.Search for more papers by this author First published: November 1987 https://doi.org/10.1111/j.1749-6632.1987.tb23864.xCitations: 7AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume506, Issue1Biochemical Engineering VNovember 1987Pages 663-668 RelatedInformation DA - 1987/11// PY - 1987/11// DO - 10.1111/j.1749-6632.1987.tb23864.x VL - 506 IS - 1 Biochemical E SP - 663-668 J2 - Ann NY Acad Sci LA - en OP - SN - 0077-8923 1749-6632 UR - http://dx.doi.org/10.1111/j.1749-6632.1987.tb23864.x DB - Crossref ER - TY - JOUR TI - Development of a defined medium and two-step culturing method for improved exotoxin A yields from Pseudomonas aeruginosa AU - Blumentals, I I AU - Kelly, R M AU - Gorziglia, M AU - Kaufman, J B AU - Shiloach, J T2 - Applied and Environmental Microbiology AB - A two-step method is described for the production of exotoxin A by Pseudomonas aeruginosa in which a defined growth medium is modified for the toxin production phase. As a result, specific exotoxin A yields comparable to those obtained with complex media were achieved. In the development of this two-step process, several divalent metallic cations (Ca2+, Cu2+, and Mn2+), in addition to iron, were found to inhibit the yield of exotoxin A while Ca2+ and glycerol were found to increase yields. Northern blot analysis of total RNA isolates suggests that these effects on exotoxin A yields are based on events at the transcription level. DA - 1987/9// PY - 1987/9// DO - 10.1128/aem.53.9.2013-2020.1987 VL - 53 IS - 9 SP - 2013-2020 J2 - Appl Environ Microbiol LA - en OP - SN - 0099-2240 1098-5336 UR - http://dx.doi.org/10.1128/aem.53.9.2013-2020.1987 DB - Crossref ER - TY - JOUR TI - Use of epifluorescence microscopy for characterizing the activity ofThiobacillus Ferrooxidans on iron pyrite AU - Yeh, Timothy Y. AU - Godshalk, John R. AU - Olson, Gregory J. AU - Kelly, Robert M. T2 - Biotechnology and Bioengineering AB - Abstract The enumeration and characterization of microorganisms attached to solid surfaces have always presented significant difficulties. This is particularly true for micro organisms that are indigenous to coal mines and mineral deposits where metal sulfides are ubiquitous. The complications that arise are the result of the variety of inorganic compounds that are present in these environments, the harsh conditions under which the microorganisms proliferate, and the low cell densities to which they grow. The work presented here suggests that epifluorescence microscopy using acridine orange can be a useful probe to study acidophilic metal‐leaching bacteria. Experiments involving the growth of Thiobacillus ferrooxidans on iron pyrite are described which indicate a relationship between cell fluorescence color and bacterial activity. Both attached and free‐solution cell densities were determined throughout the course of the leaching process and considered along with changes in cell fluorescence color which might be associated with changes in intracellular pH. As such, epifluorescence microscopy, using acridine orange, can be used for assessing the activity of T. ferrooxidans on iron pyrite as well as resolving the controversy concerning the significance of attachment during the leaching process. DA - 1987/7// PY - 1987/7// DO - 10.1002/bit.260300119 VL - 30 IS - 1 SP - 138-146 J2 - Biotechnol. Bioeng. LA - en OP - SN - 0006-3592 1097-0290 UR - http://dx.doi.org/10.1002/bit.260300119 DB - Crossref ER - TY - JOUR TI - Growth of the extreme thermophileSulfolobus acidocaldarius in a hyperbaric helium bioreactor AU - Sturm, F. J. AU - Hurwitz, S. A. AU - Deming, J. W. AU - Kelly, R. M. T2 - Biotechnology and Bioengineering AB - Abstract The relationship between pressure and temperature as it affects microbial growth and metabolism has been examined only for a limited number of bacterial species. Because many newly‐discovered, extremely thermophilic bacteria have been isolated from pressurized environments, this relationship merits closer scrutiny. In this study, the extremely thermophilic bacterium, Sulfolobus acidocaldarius , was cultured successfully in a hyperbaric chamber containing helium and air enriched with 5% carbon dioxide. Over a pressure range of approximately 1–120 bar and a temperature range of 67–80°C, growth was achieved in a heterotrophic medium with the air mixture at partial pressures up to 3.5 bar. Helium was used to obtain the final, desired incubation pressure. No significant growth was noted above 80°C over the same range of hyperbaric pressures, or at 70°C when pressure was applied hydrostatically. Growth experiments conducted under hyperbaric conditions may provide a means to study these bacteria under simulated in situ conditions and simultaneously avoid the complications associated with hydrostatic experiments. Results indicate that hyperbaric helium bioreactors will be important in the study of extremely thermophilic bacteria that are isolated from pressurized environments. DA - 1987/6/20/ PY - 1987/6/20/ DO - 10.1002/bit.260290905 VL - 29 IS - 9 SP - 1066-1074 J2 - Biotechnol. Bioeng. LA - en OP - SN - 0006-3592 1097-0290 UR - http://dx.doi.org/10.1002/bit.260290905 DB - Crossref ER - TY - JOUR TI - Bronchoscopy and bronchoalveolar lavage in the diagnosis of lower respiratory disease in the dog and cat AU - Hawkins, E.C. AU - DeNicola, D.B. T2 - Purdue Veterinary Notes DA - 1987/// PY - 1987/// VL - 155 SP - 3–4 ER - TY - JOUR TI - Le lupus érythémateux discoïde du chien: à propos de 22 observations. (Discoid lupus erythematosus in the dog: 22 cases) AU - Olivry, T. AU - Alhaidari, Z. AU - Carlotti, D. AU - Guaguère, E. AU - Régnier, A. AU - Hubert, B. AU - Magnol, J.P. AU - Oksman, F. T2 - Pratique Médicale et Chirurgicale de l’Animal de Compagnie DA - 1987/// PY - 1987/// VL - 22 SP - 205–214 ER - TY - CONF TI - Avian cryptosporidiosis ‐ An update AU - Ley, D.H. AU - Guy, J.S. AU - Levy, M.G. AU - Bermudez, A. AU - Barnes, H.J. AU - Gerig, T.M. T2 - Western Poultry Disease Conference C2 - 1987/// C3 - Proceedings of the Western Poultry Disease Conference DA - 1987/// PY - 1987/// VL - 36 SP - 49–50 ER - TY - JOUR TI - Antibodies to spotted fever-group rickettsiae in dogs in North Carolina AU - Breitschwerdt, E.B. AU - Moncol, D.J. AU - Corbett, W.T. AU - MacCormack, JN AU - Burgdorfer, W AU - Ford, RB AU - Levy, MG T2 - American Journal of Veterinary Research DA - 1987/// PY - 1987/// VL - 48 IS - 10 SP - 1436-1440 ER - TY - JOUR TI - Rhinitis, pneumonia, and defective neutrophil function in the doberman pinscher AU - Breitschwerdt, E.B. AU - Brown, T.T. AU - De Buysscher, EV AU - Andersen, BR AU - Thrall, DE AU - Hager, E AU - Ananaba, G AU - Degen, MA AU - Ward, MD T2 - American Journal of Veterinary Research DA - 1987/// PY - 1987/// VL - 48 IS - 7 SP - 1054-1062 ER - TY - JOUR TI - Antibody activity to babesia canis in dogs in North Carolina AU - Levy, M.G. AU - Breitschwerdt, E.B. AU - Moncol, D.J. T2 - American Journal of Veterinary Research DA - 1987/// PY - 1987/// VL - 48 IS - 3 SP - 339-341 ER - TY - RPRT TI - An integrated data base management program for documentation of ulcerative disease syndrome: the ulcerative disease regional data base. Florida Department of Environmental Regulation AU - Levine, J.F. AU - Cone, R.S. AU - Sandukis, N. AU - Meir, K. AU - Lee, L.C. AU - Hand, J. A3 - Florida Department of Environmental Regulation DA - 1987/// PY - 1987/// PB - Florida Department of Environmental Regulation ER - TY - JOUR TI - Cytosolic water soluble phosphodiester in vertebrate lens AU - Burt, C. T. AU - Levine, Jay T2 - Biochimica et Biophysica Acta AB - The water-soluble phosphate profiles determined by 31P nuclear magnetic resonance (NMR) of a broad range of vertebrate lenses are presented. We identify threonine ethanolamine phosphodiester (TEP) in intact lenses and lens extracts by its chemical shift and its physical and chemical characteristics. Amongst the species studied, we give the first characterization of frog lens where not only serine ethanolamine phosphodiesters (SEP) but TEP are found. DA - 1987/// PY - 1987/// DO - 10.1016/0304-4165(90)90011-k VL - 1033 SP - 189–193 ER - TY - JOUR TI - THE EFFECTS OF OXYGEN ON FERMENTATION IN TRITRICHOMONAS-FETUS KV1 AND ITS VARIANT 1MR-100 WITH DEFECTIVE HYDROGENOSOMES T2 - Journal of General Microbiology DA - 1987/// PY - 1987/// UR - https://publons.com/publon/9840978/ ER - TY - JOUR TI - Monoclonal Gammopathy Associated With Naturally Occurring Canine Ehrlichiosis AU - Breitschwerdt, Edward B. AU - Woody, Benny J. AU - Zerbe, Carole A. AU - Buysscher, Edward V. AU - Barta, Ota T2 - Journal of Veterinary Internal Medicine AB - Clinical, hematologic, and immunologic findings for 14 dogs with Ehrlichia canis monoclonal gammopathy were studied retrospectively. Epistaxis, anemia, thrombocytopenia, hypoalbuminemia, hypergammaglobulinemia, and proteinuria were documented in the majority of these dogs. The serum protein electrophoresis pattern was characterized by a distinct narrow-base monoclonal spike, by a broad-base monoclonal spike, or by a monoclonal spike superimposed on a polyclonal gammopathy. The monoclonal spike disappeared following tetracycline treatment for ehrlichiosis. The long-term prognosis following treatment was generally good. The diagnostic features of monoclonal gammopathy due to myeloma were compared with those of E. canis monoclonal gammopathy. Owing to numerous similarities in clinical, hematologic, and immunologic findings, we conclude that an E. canis antibody titer should be determined in all dogs in which a diagnosis of benign monoclonal gammopathy is contemplated or definitive evidence of myeloma, leukemia, or macroglobulinemia is lacking. DA - 1987/1// PY - 1987/1// DO - 10.1111/j.1939-1676.1987.tb01980.x VL - 1 IS - 1 SP - 2-9 LA - en OP - SN - 0891-6640 1939-1676 UR - http://dx.doi.org/10.1111/j.1939-1676.1987.tb01980.x DB - Crossref ER - TY - JOUR TI - The effects of oxygen on fermentation in Tritrichomonas foetus KV1 and its variant 1MR-100 with defective hydrogenosomes AU - Lloyd, David AU - James, Christopher J AU - Lloyd, Alun L AU - Yarlett, Nigel AU - Yarlett, Nuriza C T2 - Microbiology DA - 1987/// PY - 1987/// VL - 133 IS - 5 SP - 1181-1186 ER - TY - JOUR TI - Experimental Reproduction of Enteritis in Bobwhite Quail (Colinus virginianus) with Cryptosporidium and Reovirus AU - Guy, James S. AU - Levy, Michael G. AU - Ley, David H. AU - Barnes, H. John AU - Gerig, Thomas M. T2 - Avian Diseases AB - Five-day-old bobwhite quails were inoculated with reovirus and Cryptosporidium previously isolated from the intestinal contents of young, commercially raised bobwhite quails experiencing severe enteritis. Quails inoculated with reovirus alone did not develop clinically apparent disease, infection was localized principally in the intestinal tract, and no lesions were detected. Quails inoculated with Cryptosporidium, alone or with reovirus, developed severe enteritis with high mortality and marked growth depression. Cryptosporidia caused blunting of intestinal villi and provoked a mononuclear cell response in the lamina propria. The severity of intestinal lesions correlated with numbers of parasites. An apparent synergistic effect in dually infected quails was indicated by enhanced Cryptosporidium oocyst shedding, greater numbers of cryptosporidia in the intestinal tracts, and systemic reovirus infection. In addition, multifocal liver necrosis was detected in dually infected quails but was absent in quails infected with only reovirus or Cryptosporidium. The results suggest that Cryptosporidium promoted systemic spread of reovirus, and reovirus intensified Cryptosporidium infection, but no significant synergistic effect on mortality or weight gain was detected. The most important agent in the naturally occurring acute enteritis of bobwhite quails was Cryptosporidium. DA - 1987/10// PY - 1987/10// DO - 10.2307/1591021 VL - 31 IS - 4 SP - 713 J2 - Avian Diseases OP - SN - 0005-2086 UR - http://dx.doi.org/10.2307/1591021 DB - Crossref ER - TY - JOUR TI - Influence of Rumen Ammonia Concentration on the Rumen Degradation Rates of Barley and Maize AU - Odle, J. AU - Schaefer, D.M. T2 - British Journal of Nutrition AB - Four rumen-cannulated steers were given barley and maize diets supplemented with graded levels of an ammonium acetate solution. Animals were fed hourly from automatic feeders and water consumption was controlled to achieve steady-state conditions in the rumen. Dacron bags containing rolled barley or ground barley were incubated in the rumen of barley-fed steers, while ground maize and autoclaved maize were incubated in the rumen of maize-fed steers. Fractional degradation rates of dry matter were estimated for each cereal substrate incubated using a single-pol exponential decay model. No differences in degradation rate due to the method of feed processing were detected; however, barley was degraded at a faster rate than maize. Furthermore, the minimum rumen ammonia-nitrogen concentration required to maximize the degradation rate of barley (125 mg/l) was greater than that required to maximize the degradation rate of maize (61 mg/l). These results indicate that the optimal NH3-N concentration required to maximize the rate of grain digestion in the rumen is influenced by the chemical or structural characteristics of the grain. DA - 1987/// PY - 1987/// DO - 10.1079/BJN19870016 VL - 57 IS - 1 SP - 127-138 UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-0023233487&partnerID=MN8TOARS ER - TY - JOUR TI - The Effects of Oxygen on Fermentation in Tritrichomonas Foetus KV1 and its Variant 1MR-100 with Defective Hydrogenosomes AU - Lloyd, D. AU - James, C. J. AU - Lloyd, A. L. AU - Yarlett, N. AU - Yarlett, N. C. T2 - Microbiology AB - The effects of low concentrations of O2 on fermentation in the cattle parasite Tritrichomonas foetus KV1 and its variant 1MR-100 were compared using membrane inlet mass spectrometry to measure simultaneously and continuously ethanol, CO2 and H2. In strain KV1 glucose-supported H2 and CO2 production were stimulated by O2 concentrations < 1·4 μm but were inhibited at higher concentrations. Damped oscillatory responses in H2 production indicated the operation of a feedback control system. Measurement of the O2-dependence of O2 consumption rates confirmed the presence of a high-affinity terminal oxidase (apparent K m = 1·6 μm-O2 at 37 °C) and substrate inhibition by O2 at > 8 μm-O2. Successive periods of exposure to O2 resulted in decreased O2 scavenging capacity, as indicated by increasing apparent K m values for O2. The variant strain 1MR-100 which lacks pyruvate: ferredoxin oxidoreductase and hydrogenase showed quite different characteristics: H2 production was not detectable, ethanol formation was inhibited by O2 (K i = 1 μm) and O2-dependence of O2 consumption indicated that no high-affinity oxidase was present (apparent K m = 33 μm-O2). Progressive increases in respiration rates on repeated exposure to low O2 concentrations indicated a capacity for adaptation to aerobiosis. DA - 1987/5/1/ PY - 1987/5/1/ DO - 10.1099/00221287-133-5-1181 VL - 133 IS - 5 SP - 1181-1186 J2 - Microbiology LA - en OP - SN - 1350-0872 1465-2080 UR - http://dx.doi.org/10.1099/00221287-133-5-1181 DB - Crossref ER - TY - JOUR TI - On the pressure equation for chain molecules AU - Honnell, K. G. AU - Hall, C. K. AU - Dickman, R. T2 - The Journal of Chemical Physics AB - The pressure equation is derived for fluids containing chain molecules interacting with site–site potentials. Three models are considered: a flexible chain with harmonic potentials for bond-length and bond-angle variations, a flexible chain in the limit as the force constants for the vibrations become infinite, and a chain where bond angles and lengths are rigidly constrained to their equilibrium values. In the flexible model, the pressure is found to depend on only site–site correlations and contains both inter- and intramolecular contributions. Forces arising from angular potentials, however, do not appear in the equation. In the limit as the force constants for internal vibration become infinite, the intermolecular contribution to the pressure remains a function of pair correlations, but the intramolecular term becomes a function of chain orientation and depends on three-site correlations. The result is consistent with an expression used previously for dimers and trimers, but disagrees with a site–site equation previously used to study longer chains. The equation of state for the rigidly constrained model takes the same functional form as the equation for the infinitely stiff model. However, the two-molecule correlation function appearing in the rigid equation differs from its stiff counterpart as a result of the imposed constraints. DA - 1987/7// PY - 1987/7// DO - 10.1063/1.453562 VL - 87 IS - 1 SP - 664-674 J2 - The Journal of Chemical Physics LA - en OP - SN - 0021-9606 1089-7690 UR - http://dx.doi.org/10.1063/1.453562 DB - Crossref ER - TY - JOUR TI - Cluster-variation calculation for random-field systems: Application to hydrogen in niobium alloys AU - MacGillivray, I. R. AU - Soteros, C. E. AU - Hall, C. K. T2 - Physical Review B AB - The cluster-variation method is applied to random-field lattice systems and specifically used to model the disorder-disorder phase transition of hydrogen in niobium-molybdenum and niobium-vanadium alloys. A small concentration of molybdenum or vanadium in the niobium lattice is treated as adding at each hydrogen site a random energy with a known probability distribution. Pairwise interactions between hydrogen atoms are included out to the first fifty shells on the bcc tetrahedral interstitial lattice, allowing for the effect of site blocking for the first three shells. The results show the small and large depression in the critical temperature for ${\mathrm{Nb}}_{1\mathrm{\ensuremath{-}}\mathrm{y}}$${\mathrm{V}}_{\mathrm{y}}$${\mathrm{H}}_{\mathrm{x}}$ and ${\mathrm{Nb}}_{1\mathrm{\ensuremath{-}}\mathrm{y}}$${\mathrm{Mo}}_{\mathrm{y}}$${\mathrm{H}}_{\mathrm{x}}$, respectively, with increasing concentration of V or Mo, as observed in experiments. Comparison is made with Monte Carlo calculations of other workers with use of the same interaction parameters, and the effects of variations in the random-energy distribution are described. DA - 1987/3/1/ PY - 1987/3/1/ DO - 10.1103/physrevb.35.3545 VL - 35 IS - 7 SP - 3545-3554 J2 - Phys. Rev. B LA - en OP - SN - 0163-1829 UR - http://dx.doi.org/10.1103/physrevb.35.3545 DB - Crossref ER - TY - JOUR TI - Ordered phases in niobium hydride: Predictions using lattice-gas models AU - Hall, C.K. AU - Soteros, C. AU - Macgillivray, I. AU - Shirley, A.I. T2 - Journal of the Less Common Metals AB - A series of studies aimed at extending the Horner-Wagner model for the H-Nb system to higher hydrogen concentrations is described. Using the techniques of lattice statics, ground states analysis, sensitivity analysis, Monte Carlo simulation and the Kikuchi cluster variation method, it is concluded that both long-range electronic interactions and many-body elastic interactions are necessary to predict the ordered phases β and ϵ in addition to the disordered phases α and α'. DA - 1987/3// PY - 1987/3// DO - 10.1016/0022-5088(87)90126-3 VL - 130 SP - 319-327 J2 - Journal of the Less Common Metals LA - en OP - SN - 0022-5088 UR - http://dx.doi.org/10.1016/0022-5088(87)90126-3 DB - Crossref ER - TY - JOUR TI - Alternating Lateralized Stimulation Technique for Performing Somatosensory Evoked-Potentials AU - Goldberg, G. AU - Schmier, M.G. T2 - Electroencephalography and Clinical Neurophysiology DA - 1987/11// PY - 1987/11// VL - 67 IS - 5 SP - 102-103 ER - TY - JOUR TI - PREVENTING REGENERATION OF INFRAORBITAL AXONS DOES NOT ALTER THE GANGLIONIC OR TRANSGANGLIONIC CONSEQUENCES OF NEONATAL TRANSECTION OF THIS TRIGEMINAL BRANCH AU - CHIAIA, NL AU - HESS, PR AU - RHOADES, RW T2 - DEVELOPMENTAL BRAIN RESEARCH AB - Retrograde and transganglionic tracing with a combination of horseradish peroxidase (HRP) and wheatgerm agglutinin (WGA) — conjugated HRP (WGA-HRP) was employed to determine whether transection of the infraorbital (IO) nerve on the day of birth and prevention of regeneration by retransecting it at weekly intervals until the time of a terminal anatomical experiment had effects upon ganglion cell survival and innervation of the brainstem by this trigeminal (V) branch that differed from those which followed a single transection of the same nerve on the day of birth without any attempt to prevent peripheral regeneration of the cut axons. Counts of labelled ganglion cells and examination of the brainstem labelling produced by application of HRP and WGA-HRP to the IO nerve proximal to the point of transection(s) at 6 weeks of age demonstrated no differential effects of preventing regeneration of the cut nerve. In animals subjected to a single transection of the nerve (n = 9), we counted an average of 5001.2 (S.D. = 1286.9) labelled ganglion cells and these had an average diameter of 22.7 μm (S.D. = 6.3). In the rats (n = 9) that sustained multiple nerve cuts, the average number of labelled ganglion cells was 4447.8 (S.D. = 1060.9). The mean diameter for these primary afferent neurons was 21.5 μm (S.D. = 6.6). Neither of these values were significantly different from those from the rats subjected to a single nerve cut. The cell counts from both of these groups were significantly lower than those obtained after application of HRP and WGA-HRP to the IO nerve in normal rats (n = 3, ¯X = 12,553.3, S.D. = 1454.8), but the average cell diameter in the normals (X = 23.2, S.D. = 6.6) was not significantly greater than that in the nerve-damaged animals. The pattern of brainstem labelling observed in the rats subjected to multiple nerve cuts was the same as that in the rats which sustained a single transection of the IO nerve on the day of birth16. Very little terminal labelling was observed in nucleus principalis, subnucleus oralis, subnucleus interpolaris or the magnocellular portion of caudalis. There was, however, very heavy labelling in laminae I and II of the latter nucleus. DA - 1987/11// PY - 1987/11// DO - 10.1016/0165-3806(87)90066-6 VL - 36 IS - 1 SP - 75-88 SN - 0165-3806 ER - TY - JOUR TI - MORPHOLOGICAL-CHARACTERISTICS OF LOW-THRESHOLD PRIMARY AFFERENTS IN THE TRIGEMINAL SUBNUCLEI INTERPOLARIS AND CAUDALIS (THE MEDULLARY DORSAL HORN) OF THE GOLDEN-HAMSTER AU - CHIAIA, NL AU - HESS, PR AU - HOSOI, M AU - RHOADES, RW T2 - JOURNAL OF COMPARATIVE NEUROLOGY AB - Abstract Intra‐axonal recording and horseradish peroxidase (HRP) injection techniques were employed to define the response characteristics of low‐threshold, rapidly conducting trigeminal primary afferents and the morphological features of their axon arbors in subnucleus interpolaris and subnucleus caudalis (or the medullary dorsal horn; these last two terms are used synonomously throughout the paper). A total of 61 such afferents were characterized and recovered. Of these, ten gave rapidly adapting (RA) and 17 slowly adapting (SA type I) responses to vibrissa deflection. Twenty were sensitive to guard hair deflection and 14 were responsive to indentation of the hairy skin. The vibrissa‐sensitive primary afferents were all quite similar morphologically. Primary collaterals proceeded directly, in a radial fashion, to their zone of termination and gave rise to dense and compact arbors. These tended to be larger in the medullary dorsal horn (MDH) than in interpolaris and they also gave rise to more boutons in the former nucleus. Guard hair afferents generally had smaller arbors and gave rise to fewer boutons than vibrissa‐sensitive axons. Like vibrissa afferents, their arbor were generally circumscribed in both interpolaris and MDH, but they were larger in the latter nucleus. Skin‐sensitive afferents had arbors that tended to be somewhat larger than those of vibrissa‐ or guard‐hair‐related fibers. Unlike the other fiber types, the arbors of skin‐sensitive afferents were on average larger in interpolaris than MDH. Quantitative analysis of the morphological data from well‐filled examples from each of these four functional types verified our qualitative impressions regarding differences between interpolaris and MDH collaterals of a given fiber type. Statistical comparison of data from different functional classes indicated trends that supported our qualitative impressions, but none of these was statistically significant. The topography of the trigeminal primary afferent input to interpolaris was organized such that the head was inverted and fibers with caudal receptive fields terminated in the lateral portion of the nucleus. This was true for all of the functional afferent types that we examined. Vibrissa‐related fibers differed from nonvibrissa afferents in that they tended to avoid the most rostral portion of interpolaris. In the MDH, the primary afferent representation of the head was also inverted, but fibers with caudal facial receptive fields tended to terminate medially rather than laterally. The primary afferent input to the MDH also provided some support for the “onion‐leaf” somatotopic organization that has been suggested for this nucleus (e.g., Kunc, '70). Fibers with rostral receptive fields tended to terminate in the rostral portion of the MDH while primary afferents with caudal fields gave off most of their collaterals in the caudal MDH and the rostral portion of the first cervical segment. There were, however, a number of exceptions to this generalization. DA - 1987/10/22/ PY - 1987/10/22/ DO - 10.1002/cne.902640407 VL - 264 IS - 4 SP - 527-546 SN - 0021-9967 ER - TY - CHAP TI - Development and lesion induced reorganization of the peripheral projections of the trigeminal ganglion in rat AU - Rhoades, R. W. AU - Mooney, R. D. AU - Chiaia, N. L. AU - Szczepanik, A. M. AU - Hess, P. R. AU - Renehan, W. E. AU - Klein, B. G. AU - Nagele, R. G. AU - Jacquin, M. F. T2 - Effects of injury on trigeminal and spinal somatosensory systems A2 - L. M. Pubols, A2 - Sessle, B. J. PY - 1987/// SP - 49-60 PB - New York: Alan R. Liss, Inc. SN - 0845127322 ER - TY - JOUR TI - THE DETERMINATION OF PLATINUM IN BIOLOGICAL-MATERIALS BY ELECTROTHERMAL ATOMIC-ABSORPTION SPECTROSCOPY AU - MCGAHAN, MC AU - TYCZKOWSKA, K T2 - SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY AB - Methods for determination of platinum in body tissues and fluids by electrothermal atomic absorption spectroscopy are described. Serum and urine could be analyzed without pretreatment or dilution. Wet and dry ashing techniques for tissue digestion were compared. Dry ashing tissues in a furnace resulted in significant and unexplained losses of analyte, whereas there was complete recovery of platinum added to the tissues when the tissues were wet ashed. The wet ashing technique is fast and convenient and requires minimal sample treatment. DA - 1987/// PY - 1987/// DO - 10.1016/0584-8547(87)80122-2 VL - 42 IS - 5 SP - 665-668 SN - 0584-8547 ER - TY - JOUR TI - CYSTEINE - AN EFFECTIVE MATRIX MODIFIER FOR DETERMINATION OF GOLD IN BIOLOGICAL SAMPLES BY ELECTROTHERMAL ATOMIC-ABSORPTION SPECTROPHOTOMETRY AU - MATTHEWS, DO AU - MCGAHAN, MC T2 - SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY AB - A method was developed for determination of gold in body fluids and tissues using electrothermal atomic absorption spectrophotometry with Zeeman effect background correction. There was excellent recovery of gold added to undiluted blood plasma. However, there was generally poor recovery of gold added to the intraocular fluids and tissue digests. Addition of plasma proteins and amino acids as matrix modifiers increased both the absorbance signal of the standard and the recovery of gold added to intraocular fluids and tissue digests. Cysteine (20 mgml) was found to be the most effective matrix modifier in all cases. This signal increasing effect may be limited to gold since neither protein nor amino acids were effective in increasing absorbance signals of other elements, including Fe, Pb and Cu. DA - 1987/// PY - 1987/// DO - 10.1016/0584-8547(87)80101-5 VL - 42 IS - 7 SP - 909-913 SN - 0584-8547 ER - TY - JOUR TI - Rectal transmission of bovine leukemia virus in cattle and sheep AU - Henry, E. AU - Levine, J. F. AU - Coggins, L. T2 - American Journal of Veterinary Research DA - 1987/// PY - 1987/// VL - 48 SP - 634-636 ER - TY - JOUR TI - Pathologic manifestations of experimental Babesia microti infection in hamsters AU - Cullen, J. AU - Levine, J. F. T2 - Laboratory Animal Science DA - 1987/// PY - 1987/// VL - 37 SP - 640-643 ER - TY - JOUR TI - A green algal (Chlorophycophytal) infection of the exoskeleton and associated organ structures in the horseshoe crab, Limulus polyphemus AU - Leibovitz, L. AU - Lewbart, G. A. T2 - Biological Bulletin (Lancaster, Pa.) DA - 1987/// PY - 1987/// VL - 173 SP - 430 ER - TY - JOUR TI - Superoxide anion stimulates arachidonic acid dependent secretion of mucin by explant and cell cultures of rodent respiratory epithelium. AU - Adler, K. B. AU - Repine, J. R. T2 - American Review of Respiratory Disease DA - 1987/// PY - 1987/// VL - 135 SP - A164 ER - TY - JOUR TI - PLATELET-ACTIVATING-FACTOR STIMULATES SECRETION OF MUCIN BY EXPLANTS OF RODENT AIRWAYS IN ORGAN-CULTURE AU - ADLER, KB AU - SCHWARZ, JE AU - ANDERSON, WH AU - WELTON, AF T2 - EXPERIMENTAL LUNG RESEARCH AB - Platelet activating factor (PAF; 1-o-alkyl-2-0-acetyl-sn-glycero-3-phosphocholine-3-phosphocholine) a potential mediator of anaphylaxis, stimulates secretion of mucin by explants of trachea from four separate rodent species (guinea pig, rat, rabbit, ferret) in organ culture. Enhanced secretion is not a result of cell damage or release of histamine by cells within the explants (e.g., platelets). It is inhibited by equimolar concentrations of the potent PAF-receptor antagonist, Ro 19-3704. PAF provokes production of immunoreactive peptidyl leukotrienes (ir-LTC4, LTD4, LTE4) within the explants. The stimulatory effect of PAF on mucin secretion is blocked by equimolar concentrations of nordihydroguiaretic acid (NDGA) a "mixed" inhibitor of both cyclo- and lipoxygenase pathways of arachidonic acid metabolism. Leukotrienes are localized within tracheobronchial epithelium by immunohistochemical staining, and physical removal of epithelium from explants inhibits production of leukotrienes in vitro under nonstimulated conditions and after exposure to PAF. In addition, the stimulatory effect of PAF on mucin secretion is not altered by FPL-55712, a receptor antagonist of LTD4. These results are consistent with the hypothesis that PAF stimulates secretion of mucin by activating biosynthesis of lipoxygenase products (e.g., peptidyl leukotrienes) within epithelial cells of the respiratory mucosa. DA - 1987/// PY - 1987/// DO - 10.3109/01902148709064307 VL - 13 IS - 1 SP - 25-43 SN - 0190-2148 ER - TY - JOUR TI - Modulation of rabbit airway smooth muscle responsiveness by respiratory epithelium: involvement of an inhibitory metabolite of arachidonic acid. AU - Butler, G. B. AU - Adler, K. B. AU - Evans, J. N. AU - Szarek, J. T2 - American Review of Respiratory Disease DA - 1987/// PY - 1987/// VL - 135 SP - 1099-1104 ER - TY - JOUR TI - Dosage dependent inhibition of lung injury, inflammation and fibrosis by polyethylene glycol (PEG)-conjugated catalase in rats exposed by inhalation to asbestos. AU - Mossman, B. T. AU - Marsh, J. P. AU - Gilbert R., Hardwick AU - D. H., Sesko AU - A., Weller AU - A., Hill AU - S., Shatos AU - M. A., Doherty AU - J., Adler AU - K. B., Hemenway AU - D., Mickey AU - R., Bergeron AU - M., AU - Kagan, E. T2 - American Review of Respiratory Disease DA - 1987/// PY - 1987/// VL - 135 SP - A165 ER - TY - JOUR TI - Alterations in pulmonary vascular responsiveness following hyperoxic injury to the lung. AU - Coflesky, J. T. AU - Adler, K. B. AU - Evans, J. N. T2 - Proceedings of the 27th Aspen Lung Conference. DA - 1987/// PY - 1987/// SP - 18 ER - TY - JOUR TI - Alpha smooth muscle actin distribution in developing and adult lung. AU - Leslie, K. O. AU - Woodcock-Mitchell, J. AU - Mitchell, J. AU - Adler, K. AU - Low, R. AU - Skalli, O. AU - Gabbiani, G. T2 - Journal of Cell Biology DA - 1987/// PY - 1987/// VL - 105 SP - 25a ER - TY - JOUR TI - A new chamber system for maintaining differentiated guinea pig respiratory epithelial cells between air and liquid phases. AU - Adler, K. B. AU - Schwarz, J. E. AU - Whitcutt, J. M. AU - Wu, R. T2 - Biotechniques DA - 1987/// PY - 1987/// VL - 5 SP - 462-467 ER - TY - JOUR TI - CHARACTERIZATION OF THE JUVENILE-HORMONE ESTERASES DURING EMBRYOGENESIS OF THE HOUSE CRICKET, ACHETA-DOMESTICUS AU - ROE, RM AU - CRAWFORD, CL AU - CLIFFORD, CW AU - WOODRING, JP AU - SPARKS, TC AU - HAMMOCK, BD T2 - INTERNATIONAL JOURNAL OF INVERTEBRATE REPRODUCTION AND DEVELOPMENT AB - Summary Juvenile hormone (JH) and α-naphthyl acetate (α-NA) esterase activity was measured on a daily basis during embryogenesis of the house cricket, Acheta domesticus. In eggs dissected from the lateral oviducts and embryos through blastokinesis, there were elevated levels of nonspecific JH esterase activity. The JH esterase activity could not be resolved from the α-NA esterase activity by gel filtration chromatography and the metabolism of both substrates was inhibited equally by 0,0-diisopropyl phosphorofluoridate (DFP). From blastokinesis through egg hatch, the JH esterase activity was maintained at relatively low levels and was resolved from the α-NA esterase activity by gel filtration. The α-NA esterase activity was inhibited by DFP while the JH esterase activity was relatively unaffected. Low JH titers in eggs must be maintained through blastokinesis for normal development. Elevated JH esterase activity in eggs during this period appears to have a functional role in the metabolism of maternal JH in the egg. DA - 1987/7// PY - 1987/7// DO - 10.1080/01688170.1987.10510302 VL - 12 IS - 1 SP - 57-71 SN - 0165-1269 ER - TY - PAT TI - Ecologically improved process of protecting certain crops from damage by soil-inhabiting insect pests and product produced thereby AU - Gould, Fred AU - Pour, D. C. AU - Villani, M. G. C2 - 1987/// DA - 1987/// PY - 1987/// DO - 10.1016/0734-9750(87)90865-2 ER - TY - JOUR TI - VARIANCE FUNCTION ESTIMATION AU - DAVIDIAN, M AU - CARROLL, RJ T2 - JOURNAL OF THE AMERICAN STATISTICAL ASSOCIATION AB - Abstract Heteroscedastic regression models are used in fields including economics, engineering, and the biological and physical sciences. Often, the heteroscedasticity is modeled as a function of the covariates or the regression and other structural parameters. Standard asymptotic theory implies that how one estimates the variance function, in particular the structural parameters, has no effect on the first-order properties of the regression parameter estimates; there is evidence, however, both in practice and higher-order theory to suggest that how one estimates the variance function does matter. Further, in some settings, estimation of the variance function is of independent interest or plays an important role in estimation of other quantities. In this article, we study variance function estimation in a unified way, focusing on common methods proposed in the statistical and other literature, to make both general observations and compare different estimation schemes. We show that there are significant differences in both efficiency and robustness for many common methods. We develop a general theory for variance function estimation, focusing on estimation of the structural parameters and including most methods in common use in our development. The general qualitative conclusions are these. First, most variance function estimation procedures can be looked upon as regressions with “responses” being transformations of absolute residuals from a preliminary fit or sample standard deviations from replicates at a design point. Our conclusion is that the former is typically more efficient, but not uniformly so. Second, for variance function estimates based on transformations of absolute residuals, we show that efficiency is a monotone function of the efficiency of the fit from which the residuals are formed, at least for symmetric errors. Our conclusion is that one should iterate so that residuals are based on generalized least squares. Finally, robustness issues are of even more importance here than in estimation of a regression function for the mean. The loss of efficiency of the standard method away from the normal distribution is much more rapid than in the regression problem. As an example of the type of model and estimation methods we consider, for observation-covariate pairs (Yi, xi ), one may model the variance as proportional to a power of the mean response, for example, Where f(xi , β) is the possibly nonlinear mean function and θ is the structural parameter of interest. “Regression methods” for estimation of θ and σ based on residuals for some regression fit involve minimizing a sum of squares where some function T of the ‖ri ‖ plays the role of the “responses” and an appropriate function of the variance plays the role of the “regression function.” For example, if T(x) = x 2, the responses would be ri 2, and the form of the regression function would be suggested by the aproximate fact . One could weight the sum of squares appropriately by considering the approximate variance of ri 2. For the case of replication at each xi , some methods suggest replacing the ri , in the function T by sample standard deviations at each xi . Other functions T, such as T(x) = x or log x have also been proposed. DA - 1987/12// PY - 1987/12// DO - 10.2307/2289384 VL - 82 IS - 400 SP - 1079-1091 SN - 0162-1459 ER - TY - JOUR TI - Magnetic resonance studies on the noncyclic phosphodiesters and other lipid related metabolites AU - Burt, C. T. AU - Hill, C. H. AU - London, R. E. AU - Levine, J. F. T2 - FAEEB DA - 1987/// PY - 1987/// VL - 1 SP - 22-31 ER -