TY - MGZN TI - A Battle for Survival AU - Nettifee, J.A. T2 - Dutchess Magazine DA - 1990/8// PY - 1990/8// SP - 32–41 ER - TY - JOUR TI - Metabolism of medium- and long-chain fatty acids by isolated hepatocytes from small-for-gestational-age and appropriate-for-gestational-age piglets AU - Odle, J. AU - Benevenga, N.J. AU - Crenshaw, T.D. T2 - FASEB Journal DA - 1990/// PY - 1990/// VL - 4 SP - A658 ER - TY - RPRT TI - Do small for gestational age pigs need a different energy source than appropriate for gestational age pigs? AU - Benevenga, N.J. AU - Odle, J. AU - Crenshaw, T.D. A3 - University of Wisconsin-Madison DA - 1990/// PY - 1990/// SP - 1-3 PB - University of Wisconsin-Madison ER - TY - JOUR TI - Cytologic analysis of tracheal wash specimens and bronchoalveolar lavage fluid in the diagnosis of mycotic infections in dogs. T2 - Journal of the American Veterinary Medical Association DA - 1990/7/1/ PY - 1990/7/1/ ER - TY - CONF TI - Lyme disease in North Carolina AU - Apperson, C.S. AU - Levine, J.F. AU - Nicholson, W.L. T2 - Entomological Society of America C2 - 1990/12// CY - San Antonio, Texas DA - 1990/12// PY - 1990/12// ER - TY - CONF TI - Dermatological Diseases Affecting Fishes of the Pamlico River Estuary, North Carolina AU - Noga, E.J. AU - Levine, J.F. AU - Berkhoff, H.A. AU - Dykstra, M.J. AU - Hawkins, J.H. T2 - International Association for Aquatic Animal Medicine C2 - 1990/5// CY - San Antonio, Texas DA - 1990/5// PY - 1990/5// ER - TY - CONF TI - Ulcerative mycosis in Atlantic menhaden (Brevoortia tyrannus) Pamlico Estuary During 1988 AU - Levine, J.F. AU - Hawkins, J.H. AU - Dykstra, M.J. AU - Camp, J. AU - Cone, R.S. AU - Markwardt, N.M. T2 - American Fisheries Society Meeting C2 - 1990/7// CY - Annapolis, Maryland DA - 1990/7// PY - 1988/7// ER - TY - CONF TI - Ulcerative Lesions on Finfish in the Tar-Pamlico River AU - Levine, J.F. AU - Dykstra, M.J. AU - Noga, E.J. AU - Cone, R.S. AU - Hawkins, J.H. AU - Moye, D.W. T2 - 10th Biennial International Estuarine Research Federation Conference C2 - 1990/10// CY - Baltimore, Maryland DA - 1990/10// PY - 1990/10// ER - TY - CONF TI - Dynamics of Atlantic Menhaden (Brevoortia tyrannus) Infection with Ulcerative Mycosis AU - Levine, J.F. AU - Dykstra, M.J. AU - Noga, E.J. AU - Cone, R.S. AU - Hawkins, J.H. AU - Moye, D.W. T2 - 10th Biennial International Estuarine Research Federation Conference C2 - 1990/10// CY - Baltimore, Maryland DA - 1990/10// PY - 1990/10// ER - TY - CONF TI - Lyme disease in Virginia and North Carolina AU - Sonenshine, D.E. AU - Levine, J.F. AU - Jenkins, S. AU - Nicholson, W. AU - MacCormack, J.N. AU - Turner, R. T2 - Entomological Society of America East Branch Conference C2 - 1990/10// CY - Providence, Rhode Island DA - 1990/10// PY - 1990/10// ER - TY - JOUR TI - Reptilian and avian follicular hierarchies: models for the study of ovarian development. AU - Etches, RJ AU - Petitte, JN T2 - The Journal of experimental zoology. Supplement : published under auspices of the American Society of Zoologists and the Division of Comparative Physiology and Biochemistry AB - The presence of an ovarian follicular hierarchy is a characteristic feature of reptiles and birds. The hierarchy contains follicles at all stages of maturation and therefore, varying degrees of sensitivity to the ovulation-inducing effects of the gonadotropins. In the hen, ovulability is gained as the ability of the follicle to produce androgens and estrogens declines and the ability to produce progesterone increases. In the mature follicle, the granulosa cells are the site of progesterone production whereas the theca cells produce androgens and estrogens. Small follicles that have not yet been (and may never be) recruited into the yolk-filled hierarchy are the major producers of androgens and estrogens within the ovary. In reptiles the ovarian follicular hierarchy includes non-vitellogenic follicles and in some species includes follicles destined to become atretic. These two features distinguish the reptiles from the birds and provide experimental biologists with a unique model to investigate the physiological events that regulate the most common fate of ovarian follicles, atresia. DA - 1990/1// PY - 1990/1// DO - 10.1002/jez.1402560419 UR - http://europepmc.org/abstract/med/1974772 ER - TY - JOUR TI - Production of somatic and germline chimeras in the chicken by transfer of early blastodermal cells. AU - Petitte, JN AU - Clark, ME AU - Liu, G AU - Verrinder, Gibbins AM AU - Etches, RJ T2 - Development (Cambridge, England) DA - 1990/1// PY - 1990/1// VL - 1 UR - http://europepmc.org/abstract/med/2351062 ER - TY - CHAP TI - Microbiological Processing of Coal AU - Olson, G.J. AU - Kelly, R.M. T2 - Biotechnology Applied to Fossil Fuels A2 - Wise, D.L. PY - 1990/// PB - Marcel Dekker ER - TY - RPRT TI - Specific Biocatalysis for Coal Sulfur Speciation and Removal AU - Kelly, R.M. AU - Olson, G.J. DA - 1990/5// PY - 1990/5// M1 - ER/GS-6624 M3 - Electric Power Research Institute Interim Report SN - ER/GS-6624 ER - TY - JOUR TI - Microorganisms at extreme temperatures and pressures: Engineering insights AU - Clark, D.S. AU - Kelly, R.M. T2 - CHEMTECH DA - 1990/// PY - 1990/// VL - 20 IS - 11 SP - 641-648 ER - TY - JOUR TI - Hydrogen-Sulfur Autotrophy in the Hyperthermophilic Archaebacterium,Pyrodictium brockii AU - Pihl, T.D. AU - Schicho, R.N. AU - Black, L.K. AU - Schulman, B.A. AU - Maier, R.J. AU - Kelly, R.M. T2 - Biotechnology and Genetic Engineering Reviews AB - Abstract : This work focused on the elucidation of physiological aspects of the hyperthermophilic archaea Pyrodictium brockii and Pyrococcus furiosus. Continuous cultivation methods were developed for both organisms to facilitate metabolic studies. For P. furiosus, we demonstrated the bioenergetic importance of a novel glycolytic pathway centered around a previously reported tungsten- requiring oxido-reductase. We also showed that sulfur reduction is implicated in P. furiosus bioenergetics and examined this issue in maltose-limited cultures. Patterns of regulation were followed through the induction of several enzyme activities involved in the organism's energetics. hyperthermophiles, microbial physiology. DA - 1990/11// PY - 1990/11// DO - 10.1080/02648725.1990.10647874 VL - 8 IS - 1 SP - 345-378 J2 - Biotechnology and Genetic Engineering Reviews LA - en OP - SN - 0264-8725 2046-5556 UR - http://dx.doi.org/10.1080/02648725.1990.10647874 DB - Crossref ER - TY - JOUR TI - Purification and characterization of an alpha-glucosidase from a hyperthermophilic archaebacterium, Pyrococcus furiosus, exhibiting a temperature optimum of 105 to 115 degrees C AU - Costantino, H R AU - Brown, S H AU - Kelly, R M T2 - Journal of Bacteriology AB - Pyrococcus furiosus is a strictly anaerobic hyperthermophilic archaebacterium with an optimal growth temperature of about 100 degrees C. When this organism was grown in the presence of certain complex carbohydrates, the production of several amylolytic enzymes was noted. These enzymes included an alpha-glucosidase that was located in the cell cytoplasm. This alpha-glucosidase has been purified 310-fold and corresponded to a protein band of 125 kilodaltons as resolved by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme exhibited optimum activity at pH 5.0 to 6.0 and over a temperature range of 105 to 115 degrees C. Kinetic analysis conducted at 108 degrees C revealed hydrolysis of the substrates p-nitrophenyl-alpha-D-glucopyranoside (PNPG), methyl-alpha-D-glucopyranoside, maltose, and isomaltose. Trace activity was detected towards p-nitrophenyl-beta-D-glucopyranoside, and no activity could be detected towards starch or sucrose. Inhibition studies conducted at 108 degrees C with PNPG as the substrate and maltose as the inhibitor yielded a Ki for maltose of 14.3 mM. Preincubation for 30 min at 98 degrees C in 100 mM dithiothreitol and 1.0 M urea had little effect on enzyme activity, whereas preincubation in 1.0% sodium dodecyl sulfate and 1.0 M guanidine hydrochloride resulted in significant loss of enzyme activity. Purified alpha-glucosidase from P. furiosus exhibited remarkable thermostability; incubation of the enzyme at 98 degrees C resulted in a half life of nearly 48 h. DA - 1990/7// PY - 1990/7// DO - 10.1128/jb.172.7.3654-3660.1990 VL - 172 IS - 7 SP - 3654-3660 J2 - J Bacteriol LA - en OP - SN - 0021-9193 1098-5530 UR - http://dx.doi.org/10.1128/jb.172.7.3654-3660.1990 DB - Crossref ER - TY - JOUR TI - Role of Polysulfides in Reduction of Elemental Sulfur by the Hyperthermophilic Archaebacterium Pyrococcus furiosus AU - Blumentals, I. I. AU - Itoh, M. AU - Olson, G. J. AU - Kelly, R. M. T2 - Applied and Environmental Microbiology AB - Polysulfides formed through the breakdown of elemental sulfur or other sulfur compounds were found to be reduced to H 2 S by the hyperthermophilic archaebacterium Pyrococcus furiosus during growth. Metabolism of polysulfides by the organism was dissimilatory, as no incorporation of 35 S-labeled elemental sulfur was detected. However, [ 35 S]cysteine and [ 35 S]methionine were incorporated into cellular protein. Contact between the organism and elemental sulfur is not necessary for metabolism. The sulfide generated from metabolic reduction of polysulfides dissociates to a strong nucleophile, HS − , which in turn opens up the S 8 elemental sulfur ring. In addition to H 2 S, P. furiosus cultures produced methyl mercaptan in a growth-associated fashion. DA - 1990/5// PY - 1990/5// DO - 10.1128/aem.56.5.1255-1262.1990 VL - 56 IS - 5 SP - 1255-1262 J2 - Appl Environ Microbiol LA - en OP - SN - 0099-2240 1098-5336 UR - http://dx.doi.org/10.1128/aem.56.5.1255-1262.1990 DB - Crossref ER - TY - JOUR TI - Characterization of Amylolytic Enzyme Activities Associated with the Hyperthermophilic Archaebacterium Pyrococcus furiosus AU - Brown, Stephen H. AU - Costantino, Henry R. AU - Kelly, Robert M. T2 - Applied and Environmental Microbiology AB - The hyperthermophilic archaebacterium Pyrococcus furiosus produces several amylolytic enzymes in response to the presence of complex carbohydrates in the growth medium. These enzyme activities, α-glucosidase, pullulanase, and α-amylase, were detected in both cell extracts and culture supernatants. All activities were characterized by temperature optima of at least 100°C as well as a high degree of thermostability. The existence of this collection of activities in P. furiosus suggests that polysaccharide availability in its growth environment is a significant aspect of the niche from which it was isolated. DA - 1990/7// PY - 1990/7// DO - 10.1128/aem.56.7.1985-1991.1990 VL - 56 IS - 7 SP - 1985-1991 J2 - Appl Environ Microbiol LA - en OP - SN - 0099-2240 1098-5336 UR - http://dx.doi.org/10.1128/aem.56.7.1985-1991.1990 DB - Crossref ER - TY - JOUR TI - Characterization of sodium dodecyl sulfate-resistant proteolytic activity in the hyperthermophilic archaebacterium Pyrococcus furiosus AU - Blumentals, I I AU - Robinson, A S AU - Kelly, R M T2 - Applied and Environmental Microbiology AB - Cell extracts from Pyrococcus furiosus were found to contain five proteases, two of which (S66 and S102) are resistant to sodium dodecyl sulfate (SDS) denaturation. Cell extracts incubated at 98 degrees C in the presence of 1% SDS for 24 h exhibited substantial cellular proteolysis such that only four proteins could be visualized by amido black-Coomassie brilliant blue staining of SDS-polyacrylamide gels. The SDS-treated extract retained 19% of the initial proteolytic activity as represented by two proteases, S66 (66 kilodaltons [kDa]) and S102 (102 kDa). Immunoblot analysis with guinea pig sera containing antibodies against protease S66 indicated that S66 is related neither to S102 nor to the other proteases. The results of this analysis also suggest that S66 might be the hydrolysis product of a 200-kDa precursor which does not have proteolytic activity. The 24-h SDS-treated extract showed unusually thermostable proteolytic activity; the measured half-life at 98 degrees C was found to be 33 h. Proteases S66 and S102 were also resistant to denaturation by 8 M urea, 80 mM dithiothreitol, and 5% beta-mercaptoethanol. Purified protease S66 was inhibited by phenylmethylsulfonyl fluoride and diisopropyl fluorophosphate but not by EDTA, ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid, or iodoacetic acid. These results indicate that S66 is a serine protease. Amino acid ester hydrolysis studies showed that protease S66 was hydrolytically active towards N-benzoyl-L-arginine ethyl ester. DA - 1990/7// PY - 1990/7// DO - 10.1128/aem.56.7.1992-1998.1990 VL - 56 IS - 7 SP - 1992-1998 J2 - Appl Environ Microbiol LA - en OP - SN - 0099-2240 1098-5336 UR - http://dx.doi.org/10.1128/aem.56.7.1992-1998.1990 DB - Crossref ER - TY - JOUR TI - EFFECT OF HETEROCYCLIC AMINE ADDITIVES ON THE ABSORPTION RATES OF CARBONYL SULFIDE AND CARBON DIOXIDE IN AQUEOUS METHYLDIETHANOLAMINE SOLUTIONS AU - Reilly, J.T. AU - Schubert, C.N. AU - Lindner, J.R. AU - Donohue, M.D. AU - Kelly, R.M. T2 - Chemical Engineering Communications AB - Absorption rates of carbonyl sulfide and carbon dioxide into aqueous methyldiethanolamine solutions with and without heterocyclic amine additives were measured in a stirred cell apparatus. All of the heterocyclic amine additives catalyzed COS absorption more strongly than that for CO2. In instances in which absorption rates were improved by the additives, those with the lowest pKb's were the most effective, although steric factors also apparently influence reaction kinetics. DA - 1990/6// PY - 1990/6// DO - 10.1080/00986449008911445 VL - 93 IS - 1 SP - 181-191 J2 - Chemical Engineering Communications LA - en OP - SN - 0098-6445 1563-5201 UR - http://dx.doi.org/10.1080/00986449008911445 DB - Crossref ER - TY - JOUR TI - The Hyperthermophilic Archaebacterium, Pyrococcus furiosus. AU - Blumentals, I. I. AU - Brown, S. H. AU - Schicho, R. N. AU - Skaja, A. K. AU - Costantino, H. R. AU - Kelly, R. M. T2 - Annals of the New York Academy of Sciences AB - Annals of the New York Academy of SciencesVolume 589, Issue 1 p. 301-314 The Hyperthermophilic Archaebacterium, Pyrococcus furiosus Development of Culturing Protocols, Perspectives on Scaleup, and Potential Applicationsa I. I. BLUMENTALS, I. I. BLUMENTALS Department of Chemical Engineering Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this authorS. H. BROWN, S. H. BROWN Department of Chemical Engineering Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this authorR. N. SCHICHO, R. N. SCHICHO Department of Chemical Engineering Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this authorA. K. SKAJA, A. K. SKAJA Department of Chemical Engineering Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this authorH. R. COSTANTINO, H. R. COSTANTINO Department of Chemical Engineering Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this authorR. M. KELLY, R. M. KELLY Department of Chemical Engineering Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this author I. I. BLUMENTALS, I. I. BLUMENTALS Department of Chemical Engineering Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this authorS. H. BROWN, S. H. BROWN Department of Chemical Engineering Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this authorR. N. SCHICHO, R. N. SCHICHO Department of Chemical Engineering Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this authorA. K. SKAJA, A. K. SKAJA Department of Chemical Engineering Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this authorH. R. COSTANTINO, H. R. COSTANTINO Department of Chemical Engineering Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this authorR. M. KELLY, R. M. KELLY Department of Chemical Engineering Johns Hopkins University Baltimore, Maryland 21218Search for more papers by this author First published: May 1990 https://doi.org/10.1111/j.1749-6632.1990.tb24254.xCitations: 20 a This work was supported in part by the National Science Foundation (Grant No. CBT-8813608). AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat References 1 STETTER, K. O. 1986. In Thermophiles: General, Molecular, and Applied Microbiology. T. D. Brock, Ed. Wiley. New York . 2 Kelly, R. M. & J. W. Deming. 1988. Biotechnol. Prog. 4: 47–62. 3 Fiala, G. & K. O. Stetter. 1986. Arch. Microbiol. 145: 56–60. 4 Zillig, W., I. Holz, H. P. Klenk, J. Trent, S. Wunderl, D. Janekovic, E. Imsel & B. Haas. 1987. Syst. Appl. Microbiol. 9: 62–70. 5 Malik, B., W-W. Su, H. L. Wald, I. I. Blumentals & R. M. Kelly. Biotechnol. Bioeng. 34: 1050–1057. 6 Kester, D. R., I. W. Duedall, D. N. Connore & R. M. Pytkowicz. 1967. Limnol. Oceanogr. 12: 176–178. 7 Hobbie, J. E., R. J. Daley & S. Jasper. 1977. Appl. Environ. Microbiol. 33: 1225–1228. 8 Jannasch, H. W., C. O. Wirsen, S. J. Molyneaux & T. A. Langworthy. 1988. Appl. Environ. Microbiol. 54: 1203–1209. 9 Parameswaran, A. K., W-W. Su, R. N. Schicho, C. N. Provan, B. Malik & R. M. Kelly. 1988. Appl. Biochem. Biotechnol. 18: 53–73. 10 Stetter, K. O., H. Konig & E. Stackebrandt. 1983. Syst. Appl. Microbiol. 4: 535–551. 11 Ng, T. K. & W. F. Kenealy. 1986. In Thermophiles: General, Molecular, and Applied Microbiology. T. D. Brock, Ed. Wiley. New York . 12 Smith, P. F., T. A. Langworthy & M. R. Smith. 1975. J. Bacteriol. 124: 884–892. 13 Brown, S. H., I. I. Blumentals & R. M. Kelly. 1988. AIChE Annual Meeting, Washington, District of Columbia, abstract no. 149k. 14 Schicho, R. N., S. H. Brown, G. J. Olson, E. J. Parks & R. M. Kelly. 1989. Fuel 68: 1368–1375. 15 Pihl, T., R. N. Schicho, R. M. Kelly & R. J. Maier. 1988. Proc. Natl. Acad. Sci. U.S.A. 86: 138–141. 16 Adams, M. W. W. 1988. Personal communication. Department of Biochemistry, University of Georgia . 17 Skaja, A. K., R. Lessick, C. B. Anfinsen & R. M. Kelly. 1988. AIChE Annual Meeting, Washington, District of Columbia, abstract no. 23o. 18 Laderman, K. & C. B. Anfinsen. 1988. Personal communication. Department of Biology, Johns Hopkins University . Citing Literature Volume589, Issue1Biochemical EngineeringMay 1990Pages 301-314 ReferencesRelatedInformation DA - 1990/5// PY - 1990/5// DO - 10.1111/j.1749-6632.1990.tb24254.x VL - 589 IS - 1 Biochemical E SP - 301-314 J2 - Ann NY Acad Sci LA - en OP - SN - 0077-8923 1749-6632 UR - http://dx.doi.org/10.1111/j.1749-6632.1990.tb24254.x DB - Crossref ER - TY - JOUR TI - Cytologic analysis of tracheal wash specimens and bronchoalveolar lavage fluid in the diagnosis of mycotic infections in dogs AU - Hawkins, E.C. AU - DeNicola, D.B. T2 - Journal of the American Veterinary Medical Association DA - 1990/7// PY - 1990/7// VL - 197 IS - 1 SP - 79–83 ER - TY - JOUR TI - Canine muscle disorders AU - Scott‐Moncrieff, J.C. AU - Hawkins, E.C. AU - Cook, J.R., Jr. T2 - Compendium on Continuing Education Practicing Veterinarian DA - 1990/// PY - 1990/// VL - 12 IS - 1 SP - 31–39 ER - TY - CONF TI - Chronic viral upper respiratory disease in cats AU - Hawkins, E.C. C2 - 1990/// C3 - Sheba Feline Medicine Symposium DA - 1990/// SP - 17–26 PB - Kal Kan Foods, Inc ER - TY - JOUR TI - Dermatologie et hormones sexuelles chez les carnivores domestiques. Deuxième partie: étude clinique. (Dermatology and sex hormones in small animals. Second part: clinical studies) AU - Hubert, B. AU - Olivry, T. T2 - Pratique Médicale et Chirurgicale de l’Animal de Compagnie DA - 1990/// PY - 1990/// VL - 25 SP - 483–502 ER - TY - JOUR TI - Dermatologie et hormones sexuelles chez les carnivores domestiques. Première partie: physiopathologie. (Dermatology and sex hormones in small animals. First part: physiopathology) AU - Hubert, B. AU - Olivry, T. T2 - Pratique Médicale et Chirurgicale de l’Animal de Compagnie DA - 1990/// PY - 1990/// VL - 25 SP - 477–482 ER - TY - JOUR TI - Feline ocular disease and the rhinotracheitis virus AU - Nasisse, M.P. AU - Guy, J.S. T2 - Veterinary Medicine Report DA - 1990/// PY - 1990/// VL - 1 IS - 2 SP - 155–165 ER - TY - JOUR TI - Reactivation of latent pseudorabies virus infection in vaccinated commercial sows AU - Cowen, P. AU - Li, S. AU - Guy, J.S. AU - Erickson, G.A. AU - Blanchard, D. T2 - American Journal of Veterinary Research DA - 1990/3// PY - 1990/3// VL - 51 IS - 3 SP - 354–358 ER - TY - JOUR TI - Acute-renal-failure after methylene-blue infusion in a dog AU - Osuna, D.J. AU - Armstrong, P.J. AU - Duncan, D.E. AU - Breitschwerdt, E.B. T2 - Journal of the American Animal Hospital Association DA - 1990/// PY - 1990/// VL - 26 IS - 4 SP - 410-412 ER - TY - JOUR TI - An epizootic of peroneal and tibial neuropathy in walker hound pups AU - Jans, H.E. AU - Kornegay, J.N. AU - Breitschwerdt, E.B. AU - Armstrong, P.J. T2 - Journal of American Veterinary Medical Association DA - 1990/// PY - 1990/// VL - 197 IS - 4 SP - 498-500 ER - TY - JOUR TI - Vascular permeability and coagulation during rickettsia rickettsii infection in dogs AU - Davidson, M.G. AU - Breitschwerdt, E.B. AU - Walker, D.H. AU - Levy, MG AU - Carlson, CS AU - Hardie, EM AU - Grindem, CA AU - Nasisse, MP T2 - American Journal of Veterinary Research DA - 1990/// PY - 1990/// VL - 51 IS - 1 SP - 165-170 ER - TY - JOUR TI - Un cas de dysautonomie féline (Feline dysautonomia: a case report) AU - Kommer, A.L. AU - Gamet, Y. AU - Olivry, T. T2 - L’Action Vétérinaire DA - 1990/4/13/ PY - 1990/4/13/ SP - 21–23 ER - TY - JOUR TI - Polyarthrite chronique évolutive féline. (Feline chronic progressive polyarthritis) AU - Poulet, H. AU - Olivry, T. T2 - L’Action Vétérinaire DA - 1990/4/20/ PY - 1990/4/20/ SP - 12–15 ER - TY - JOUR TI - Plasmocytome non secrétant chez un chien (Non secreting plasma cell myeloma in a dog) AU - Soussan, O. AU - Olivry, T. T2 - L’Action Vétérinaire DA - 1990/5/4/ PY - 1990/5/4/ SP - 19–22 ER - TY - JOUR TI - What is your diagnosis: Retropharyngeal caseous lymphadenitis in a goat AU - Jones, S.L. AU - Schumacher, J. T2 - Journal of the American Veterinary Medical Association DA - 1990/// PY - 1990/// VL - 197 SP - 395–396 ER - TY - JOUR TI - Growth hormone-responsive dermatosis: myth or reality AU - Olivry, T. T2 - Semaine Vétérinaire DA - 1990/// PY - 1990/// IS - 572 SP - 10 M3 - Editorial ER - TY - JOUR TI - Panniculus adiposus: unfairly forgotten AU - Olivry, T. T2 - Semaine Vétérinaire DA - 1990/// PY - 1990/// IS - 568 SP - 13 M3 - Editorial ER - TY - JOUR TI - Cocker seborrhea: the hopes of synthetic retinoids AU - Olivry, T. T2 - Semaine Vétérinaire DA - 1990/// PY - 1990/// IS - 584 SP - 14 M3 - Editorial ER - TY - JOUR TI - WSAVA meeting: allergology update AU - Olivry, T. T2 - Semaine Vétérinaire DA - 1990/// PY - 1990/// IS - 584 SP - 18 M3 - Editorial ER - TY - JOUR TI - Superficial necrolytic dermatitis: one for all AU - Olivry, T. T2 - Semaine Vétérinaire DA - 1990/// PY - 1990/// IS - 588 SP - 8 M3 - Editorial ER - TY - JOUR TI - Canine sebaceous tumors: toward malignancy AU - Olivry, T. T2 - Semaine Vétérinaire DA - 1990/// PY - 1990/// IS - 594 SP - 8 M3 - Editorial ER - TY - JOUR TI - Parametric spin wave testing by observation of transition processes AU - Smirnov, A.I. AU - Erhart, P. AU - Senning, B. AU - Waldner, F. T2 - Journal of Magnetism and Magnetic Materials AB - Relaxation rates and coupling coefficients of spin waves have been determined in the layered ferromagnet (CH3NH3)2CuCl4. The parameters could be measured by observing the transient response of the parallel pumped microwave absorption to rapid magnetic field changes. DA - 1990/// PY - 1990/// DO - 10.1016/0304-8853(90)90687-L VL - 92 IS - 1 SP - 116-124 UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-0025519602&partnerID=MN8TOARS ER - TY - JOUR TI - EPR tomography of the diffusion kinetic process of the formation of Ag2+ in heterogeneous catalysts AU - Yakimchenko, O.E. AU - Degtyarev, E.N. AU - Smirnov, A.I. AU - Borisov, Yu.V. AU - Bobolev, A.V. T2 - Kinetics and Catalysis DA - 1990/// PY - 1990/// VL - 31 IS - 2 pt 2 SP - 433-435 UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-0025498797&partnerID=MN8TOARS ER - TY - JOUR TI - Platelet Aggregation in Dogs Experimentally Infected with Rickettsia rickettsii AU - Grindem, C.B. AU - Corbett, W.T. AU - Levy, M.G. AU - Davidson, M.G. AU - Breitschwerdt, E.B. T2 - Veterinary Clinical Pathology AB - Summary Platelet aggregation studies were performed on nine Beagle dogs experimentally infected with Rickettsia rickettsii. Platelets from dogs with Rocky Mountain spotted fever tended to be more aggregable than controls. DA - 1990/3// PY - 1990/3// DO - 10.1111/j.1939-165x.1990.tb00537.x VL - 19 IS - 1 SP - 25-28 LA - en OP - SN - 0275-6382 1939-165X UR - http://dx.doi.org/10.1111/j.1939-165x.1990.tb00537.x DB - Crossref ER - TY - JOUR TI - Regression and calibration with nonconstant error variance AU - Davidian, Marie AU - Haaland, Perry D. T2 - Chemometrics and Intelligent Laboratory Systems AB - Davidian, M. and Haaland, P., 1990. Regression and calibration with nonconstant error variance. Chemometrics and Intelligent Laboratory Systems, 9: 231–248. Ordinary least squares regression analysis is generally inappropriate for calibration and regression problems when the usual assumption of constant variance across all observations does not hold. Estimators of regression parameters are of relatively poor quality and the resulting inference can be misleading. The use of standard data transformations is a common alternative but may not provide enough flexibility for some cases. The use of weighted regression with weights estimated from replicates is generally unreliable for reasonable sample sizes. However, when the error variance changes systematically with the mean response or other variables, generalized least squares (GLS) and variance function estimation (VFE) methods can be used. The GLS-VFE approach allows the experimenter to specify a model for the systematic change in variance, estimate unknown parameters, and to use this information to provide more efficient estimates of the regression parameters. In this tutorial, GLS-VFE methods are introduced and described in the context of regression and calibration. An example of calibration for a chemical assay is used to motivate discussion and illustrate the implementation of these methods using standard software packages. DA - 1990/12// PY - 1990/12// DO - 10.1016/0169-7439(90)80074-g VL - 9 IS - 3 SP - 231-248 J2 - Chemometrics and Intelligent Laboratory Systems LA - en OP - SN - 0169-7439 UR - http://dx.doi.org/10.1016/0169-7439(90)80074-g DB - Crossref ER - TY - JOUR TI - Estimation of variance functions in assays with possibly unequal replication and nonnormal data AU - Davidian, M. T2 - Biometrika AB - Estimation of parametric variance functions using transformations of standard deviations based on replication at each design point is common in, but not limited to, assay analysis. It is shown that ignoring unequal replication can lead to bias and inefficiency in estimation. Efficiency comparisons for different transformations for nonnormal distributions are given. A method to account for bias is described that can offer robustness to nonnormality and leads to a comparison of Gini's mean difference to sample standard deviation. A method for computing all of these estimators using standard software is described. DA - 1990/3/1/ PY - 1990/3/1/ DO - 10.1093/biomet/77.1.43 VL - 77 IS - 1 SP - 43-54 J2 - Biometrika LA - en OP - SN - 0006-3444 1464-3510 UR - http://dx.doi.org/10.1093/biomet/77.1.43 DB - Crossref ER - TY - JOUR TI - Bronchoalveolar Lavage in the Evaluation of Pulmonary Disease in the Dog and Cat AU - Hawkins, Eleanor C. AU - DeNicola, Dennis B. AU - Kuehn, Ned F. T2 - Journal of Veterinary Internal Medicine AB - Bronchoalveolar lavage is a diagnostic procedure used to obtain specimens representative of disease processes involving the deep lung. Saline is instilled into an airway in sufficient volumes to bathe the alveoli dependent on that airway. The saline is retrieved by suction along with cellular and acellular material lining the epithelial surfaces of the lung. Cytologic and microbiologic evaluation of the fluid can be used to characterize pulmonary diseases in the dog and cat. DA - 1990/9// PY - 1990/9// DO - 10.1111/j.1939-1676.1990.tb03120.x VL - 4 IS - 5 SP - 267-274 LA - en OP - SN - 0891-6640 1939-1676 UR - http://dx.doi.org/10.1111/j.1939-1676.1990.tb03120.x DB - Crossref ER - TY - JOUR TI - Allergic contact dermatitis in the dog. Principles and diagnosis. AU - Olivry, T. AU - Prélaud, P. AU - Héripret, D. AU - Atlee, B.A. T2 - The Veterinary clinics of North America. Small animal practice AB - Allergic contact dermatitis is a rare hypersensitivity disorder in the dog. Clinical diagnosis is not easy. Primary lesions are transient. Secondary lesions caused by chronic inflammation and self-trauma are commonly present on typical areas, especially in sparsely haired regions and on the feet. A presumptive diagnosis can be made based on the history, clinical signs, and positive standardized closed patch testing. Histopathology and basophil degranulation testing may also be helpful in supporting the diagnosis and in identifying the offending allergens. However, a definitive diagnosis can be made only after restriction-provocation testing. With better understanding of the pathogenesis and clinical signs, and with increased availability of diagnostic tests (especially a standardized closed patch test), increased recognition of this dermatosis is expected. DA - 1990/// PY - 1990/// DO - 10.1016/S0195-5616(90)50154-0 VL - 20 IS - 6 SP - 1443-1456 UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-0025518311&partnerID=MN8TOARS ER - TY - JOUR TI - Comparative pharmacokinetics of doxycycline in cats and dogs AU - Riond, J. L. AU - Vaden, S. L. AU - Riviere, J. E. T2 - Journal of Veterinary Pharmacology and Therapeutics AB - Riond, J.‐L., Vaden, S.L. & Riviere, J.E. Comparative pharmacokinetics of doxycycline in cats and dogs. J. vet. Pharmacol. Therap. 13, 415–424. The disposition of doxycycline hyclate was studied in six adult mixed‐breed female cats and six adult mid‐sized female dogs following a single intravenous administration of 5 mg/kg body weight. Doxycycline volume of the central compartment, area volume of distribution, volume of distribution at steady state, and total body clearance were significantly smaller in cats. The differences were attributed to more extensive binding of doxycycline to plasma protein including albumin in cats. The significant differences in the volume of distribution and total body clearance were not reflected in elimination half‐lives under the conditions of this study (sample size, inhomogeneous population). Doxycycline elimination half‐life was 4.56±0.68 (SEM)h for cats and 6.99±1.09h for dogs. Dosage regimens recommended in the veterinary literature were evaluated by the computer program PETDR. Dr J. E. Riviere, Laboratory of Toxicokinetics, Department of Anatomy, Physiological Sciences, and Radiology, College of Veterinary Medicine, North Carolina Stale University, Raleigh, NC 27606, USA. DA - 1990/12// PY - 1990/12// DO - 10.1111/j.1365-2885.1990.tb00797.x VL - 13 IS - 4 SP - 415-424 J2 - J Vet Pharmacol Ther LA - en OP - SN - 0140-7783 1365-2885 UR - http://dx.doi.org/10.1111/j.1365-2885.1990.tb00797.x DB - Crossref ER - TY - JOUR TI - Statistical-mechanical model of protein precipitation by nonionic polymer AU - Mahadevan, Hari AU - Hall, Carol K. T2 - AIChE Journal AB - Abstract A theoretical approach to predict the solubility of proteins in solutions containing nonionic polymers is presented. The effective protein‐protein interaction due to the presence of the polymer is related to the volume‐exclusion potential of Asakura and Oosawa. Statistical‐mechanical perturbation theory, as originally applied by Gast et al. to model colloidal flocculation, is used to calculate free energies, from which solubility curves for varying protein‐polymer diameter ratios are obtained. The theory correctly predicts all the trends observed in experimental studies of these systems. To explain the influence of processes parameters such as the pH and the ionic strength on protein solubility, the intermolecular potential is improved by the addition of an electrostatic interaction term. It is found that theoretical predictions of the variation in protein solubility, both with the solution pH and the ionic strength, are in accordance with experimental observations. DA - 1990/10// PY - 1990/10// DO - 10.1002/aic.690361007 VL - 36 IS - 10 SP - 1517-1528 J2 - AIChE J. LA - en OP - SN - 0001-1541 1547-5905 UR - http://dx.doi.org/10.1002/aic.690361007 DB - Crossref ER - TY - JOUR TI - Molecular-dynamics simulation results for the pressure of hard-chain fluids AU - Denlinger, Mark A. AU - Hall, Carol K. T2 - Molecular Physics AB - We present molecular-dynamics simulation results for the equation of state of one-component fluids containing freely jointed hard chains of lengths 2, 4, 8, 16 and 32, and compare these results with the predictions of the generalized Florydimer theory. In addition, we present the predictions of a modification to the generalized Flory-dimer theory which uses accurate values for the exclusion volumes of hard chains. Using a Monte Carlo integration technique, we obtain the conformationally averaged exclusion volumes, mean-squared radii of gyration and mean-squared end-to-end distances of isolated freely jointed hard chains of lengths 3, 4, 5, 6, 7, 8, 15, 16, 17, 31, 32, 33 and 64. This modification to the generalized Flory-dimer theory improves the agreement with simulation results for the longer chains at high densities, but has negligible effect at intermediate densities. DA - 1990/10/20/ PY - 1990/10/20/ DO - 10.1080/00268979000101961 VL - 71 IS - 3 SP - 541-559 J2 - Molecular Physics LA - en OP - SN - 0026-8976 1362-3028 UR - http://dx.doi.org/10.1080/00268979000101961 DB - Crossref ER - TY - JOUR TI - Interfacial tension of polyethyleneglycol-dextran-water systems: influence of temperature and polymer molecular weight AU - Forciniti, D. AU - Hall, C.K. AU - Kula, M.R. T2 - Journal of Biotechnology AB - We explore the influence that polymer molecular weight, total polymer concentration and temperature have on the interfacial tension of aqueous two-phase systems. We report the interfacial tension at 4°C, 25°C and 40°C for sixteen different PEG/Dx/water systems which consist of all possible combinations of the four PEG molecular weights 4,000, 6,000, 10,000 and 20,000 and the four Dx molecular weights 10,000, 40,000, 110,000 and 500,000. Four tie lines were measured for each system. We show that there exists a good linear correlation between the log of the interfacial tension and log of the tie line length, the difference in Dx concentration between the top and bottom phase (ΔDx ≡ [Dx]T − [Dx]B) and the difference in PEG concentration between the top and bottom phase (ΔPEG ≡ [PEG]T − [PEG]B). The linear correlation between the log of the interfacial tension and ΔDx or ΔPEG suggests the possibility of using interfacial tension as an analytical tool to measure phase composition. When systems of the same tie line length are compared, increasing the molecular weight of one of the polymers increases the interfacial tension. We find that systems closer to the critical point (short tie line length) are more sensitive to changes in polymer molecular weight than systems far away from the critical point (long tie line length). The trend of interfacial tension with temperature is less clear than the trend with polymer molecular weight. The double log curves of interfacial tension versus tie line length at different temperatures cross each other in such a way that an increase in the temperature will increase, decrease or not affect the interfacial tension depending on the tie line length that we consider. DA - 1990/11// PY - 1990/11// DO - 10.1016/0168-1656(90)90042-a VL - 16 IS - 3-4 SP - 279-296 J2 - Journal of Biotechnology LA - en OP - SN - 0168-1656 UR - http://dx.doi.org/10.1016/0168-1656(90)90042-a DB - Crossref ER - TY - JOUR TI - Niobium hydride phase behavior studied using the cluster-variation method AU - Soteros, Christine E. AU - Hall, Carol K. T2 - Physical Review B AB - We present several models for NbH phase behavior based on the cluster-variation method. The most important of these are the single-tetragonal-cell (STC) model and the twelve-cluster (TWC) model, which have as their basic clusters the single tetragonal cell and a twelve-sited cluster, respectively. The following conclusions can be drawn from our study of these models. From the STC ground-state analysis we find that, for interactions confined to the single tetragonal cell (a subset of the first 14 shells for the tetrahedral lattice), (1) it is possible to predict the \ensuremath{\beta} and \ensuremath{\zeta} phases but not the \ensuremath{\epsilon} phase, and (2) many-body interactions are needed to eliminate an extraneous pure phase (i.e., a phase that is not observed experimentally) at concentration [H]/[Nb]=0.25, assuming the \ensuremath{\beta}, \ensuremath{\epsilon}', and \ensuremath{\zeta} phases are ground states. These observations suggest that longer-range interactions than those in the STC are important for NbH phase formation and many-body interactions may be necessary to eliminate unwanted phases at lower concentrations. We have also generated phase diagrams that are qualitatively similar (in the ordered regions) to experimental NbH phase diagrams. Our study of the TWC model shows that it is more accurate in comparison with a Monte Carlo model than the existing entropy approximations of Boureau, Richards, and MacGillivray, Soteros, and Hall. Furthermore, using the TWC model it is possible to predict an \ensuremath{\alpha}-\ensuremath{\alpha}'-\ensuremath{\beta} phase envelope that compares well with the Futran and Hall Monte Carlo calculation. This is the first analytic free-energy calculation to predict the \ensuremath{\alpha}-\ensuremath{\alpha}'-\ensuremath{\beta} transition. Using the TWC model we also show that, as Shirley and Hall have conjectured, many-body interactions work to lower the \ensuremath{\alpha}-\ensuremath{\alpha}' critical temperature when compared with calculations including only pair interactions. Since pair-interaction calculations overestimate the critical temperature, this is further evidence of the importance of many-body interactions. DA - 1990/10/1/ PY - 1990/10/1/ DO - 10.1103/physrevb.42.6590 VL - 42 IS - 10 SP - 6590-6611 J2 - Phys. Rev. B LA - en OP - SN - 0163-1829 1095-3795 UR - http://dx.doi.org/10.1103/physrevb.42.6590 DB - Crossref ER - TY - JOUR TI - Exact equations of state for one-dimensional chain fluids AU - Honnell, Kevin G. AU - Hall, Carol K. T2 - Journal of Statistical Physics DA - 1990/11// PY - 1990/11// DO - 10.1007/bf01027302 VL - 61 IS - 3-4 SP - 803-842 J2 - J Stat Phys LA - en OP - SN - 0022-4715 1572-9613 UR - http://dx.doi.org/10.1007/bf01027302 DB - Crossref KW - CHAIN MOLECULE KW - EQUATION OF STATE KW - ONE DIMENSION KW - ISOTHERMAL KW - ISOBARIC ENSEMBLE ER - TY - JOUR TI - Local structure of fluids containing chain‐like molecules: Polymer reference interaction site model with a Yukawa closure AU - Yethiraj, Arun AU - Hall, Carol K. T2 - The Journal of Chemical Physics AB - The local structure of fluids composed of chain-like molecules (modeled as a pearl necklace of freely jointed hard spheres) is investigated via an integral equation approach. A Yukawa closure for the direct correlation function is used in the framework of polymer-RISM theory. This introduces two free parameters which are chosen by matching the theory’s predictions for the compressibility and the contact value of the site–site distribution function, to an equation of state and simulation data, respectively. The theory shows good agreement when compared to Monte Carlo simulations of tangent diatomics, and freely jointed 4-mers, and 8-mers. Methods for estimating the contact value of the site–site distribution function are discussed. DA - 1990/10// PY - 1990/10// DO - 10.1063/1.459650 VL - 93 IS - 7 SP - 5315-5321 J2 - The Journal of Chemical Physics LA - en OP - SN - 0021-9606 1089-7690 UR - http://dx.doi.org/10.1063/1.459650 DB - Crossref ER - TY - JOUR TI - Computer simulation studies of the growth of strained layers by molecular-beam epitaxy AU - Faux, D. A. AU - Gaynor, G. AU - Carson, C. L. AU - Hall, C. K. AU - Bernholc, J. T2 - Physical Review B AB - Two new types of discrete-space Monte Carlo computer simulation are presented for the modeling of the early stages of strained-layer growth by molecular-beam epitaxy. The simulations are more economical on computer resources than continuous-space Monte Carlo or molecular dynamics. Each model is applied to the study of growth onto a substrate in two dimensions with use of Lennard-Jones interatomic potentials. Up to seven layers are deposited for a variety of lattice mismatches, temperatures, and growth rates. Both simulations give similar results. At small lattice mismatches (\ensuremath{\lesssim}4%) the growth is in registry with the substrate, while at high mismatches (\ensuremath{\gtrsim}6%) the growth is incommensurate with the substrate. At intermediate mismatches, a transition from registered to incommensurate growth is observed which commences at the top of the crystal and propagates down to the first layer. Faster growth rates are seen to inhibit this transition. The growth mode is van der Merwe (layer-by-layer) at 2% lattice mismatch, but at larger mismatches Volmer-Weber (island) growth is preferred. The Monte Carlo simulations are assessed in the light of these results and the ease at which they can be extended to three dimensions and to more sophisticated potentials is discussed. DA - 1990/8/15/ PY - 1990/8/15/ DO - 10.1103/physrevb.42.2914 VL - 42 IS - 5 SP - 2914-2922 J2 - Phys. Rev. B LA - en OP - SN - 0163-1829 1095-3795 UR - http://dx.doi.org/10.1103/physrevb.42.2914 DB - Crossref ER - TY - JOUR TI - Site–site correlations in short chain fluids AU - Yethiraj, Arun AU - Hall, Carol K. AU - Honnell, Kevin G. T2 - The Journal of Chemical Physics AB - Intramolecular and intermolecular site–site correlations in short chain fluids are obtained via Monte Carlo simulation for volume fractions ranging between 0.05 and 0.35. The chains are modeled as pearl necklaces of freely jointed hard spheres; chains composed of 4 and 8 beads are studied. The intramolecular distribution between a pair of beads separated by a fixed number of segments along the chain is found to be remarkably independent of the position of the pair along the chain. At low densities the intermolecular site–site pair distribution function at contact is found to be much less than one due to the ‘‘correlation hole’’ effect. The contact value increases as the density is increased, and decreases as the chain length is increased. We use the intramolecular correlations measured to obtain polymer reference interaction site model predictions for the intermolecular site–site distribution function. We find that the theory accurately reproduces the local structure of the fluid, but significantly overestimates the contact value of the distribution function, especially at low densities. A comparison of freely jointed chain results with simulations of chains with fixed bond angles and torsional rotations treated in the rotational isomeric state approximation shows that the correlation hole is more pronounced in freely jointed chains. We test a superposition approximation used to evaluate the three body term in the pressure equation for chain molecules. We find that the three-body term is sizeable, and that the superposition approximation significantly underestimates the three-body contribution. DA - 1990/9/15/ PY - 1990/9/15/ DO - 10.1063/1.458728 VL - 93 IS - 6 SP - 4453-4461 J2 - The Journal of Chemical Physics LA - en OP - SN - 0021-9606 1089-7690 UR - http://dx.doi.org/10.1063/1.458728 DB - Crossref ER - TY - JOUR TI - Monte-Carlo simulation of polymers confined between flat plates AU - Yethiraj, Arun AU - Hall, Carol K. T2 - Macromolecules AB - ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMonte-Carlo simulation of polymers confined between flat platesArun Yethiraj and Carol K. HallCite this: Macromolecules 1990, 23, 6, 1865–1872Publication Date (Print):March 1, 1990Publication History Published online1 May 2002Published inissue 1 March 1990https://doi.org/10.1021/ma00208a052Request reuse permissionsArticle Views534Altmetric-Citations135LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (896 KB) Get e-Alertsclose Get e-Alerts DA - 1990/11// PY - 1990/11// DO - 10.1021/ma00208a052 VL - 23 IS - 6 SP - 1865-1872 J2 - Macromolecules LA - en OP - SN - 0024-9297 1520-5835 UR - http://dx.doi.org/10.1021/ma00208a052 DB - Crossref ER - TY - JOUR TI - Efficient techniques for computer simulations of heteroepitaxial growth AU - Carson, C. L. AU - Bernholc, J. AU - Faux, D. AU - Hall, C. K. T2 - Applied Physics Letters AB - A new discrete Monte Carlo technique suitable for simulations of the kinetics of heteroepitaxial crystal growth has been developed and tested on a 103 atom system. The technique offers sizable speed advantages over previous simulation methods and allows for realistic three-dimensional studies of the kinetics of both pseudomorphic and misfit growth modes and of the transformation between them. Elements of the method are of general utility and can also be used to substantially improve the efficiency of continuous-space Monte Carlo and molecular dynamics simulations of growth and other atomic transformations. DA - 1990/5/14/ PY - 1990/5/14/ DO - 10.1063/1.103014 VL - 56 IS - 20 SP - 1971-1973 J2 - Appl. Phys. Lett. LA - en OP - SN - 0003-6951 1077-3118 UR - http://dx.doi.org/10.1063/1.103014 DB - Crossref ER - TY - CHAP TI - Theoretical Treatment of Aqueous Two-Phase Extraction by Using Virial Expansions AU - Forciniti, Daniel AU - Hall, Carol k. T2 - ACS Symposium Series AB - A theoretical treatment of aqueous two-phase extraction at the isoelectric point is presented. We extend the constant pressure solution theory of Hill to the prediction of the chemical potential of a species in a system containing solvent, two polymers and protein. The theory leads to osmotic virial-type expansion and gives a fundamental interpretation the osmotic virial coefficients in terms of forces between species. The expansion is identical to the Edmunds-Ogston-type expression only when certain assumptions are made -- one of which is that the solvent non-interacting. The coefficients are calculated using simple excluded volume models for polymer-protein interactions and are then inserted into the expansion to predict isoelectric partition coefficients. The results are compared with trends observed experimentally for protein partition coefficients as functions of protein and polymer molecular weights. PY - 1990/1/24/ DO - 10.1021/bk-1990-0419.ch003 SP - 53-70 OP - PB - American Chemical Society SN - 0841217386 0841212759 UR - http://dx.doi.org/10.1021/bk-1990-0419.ch003 DB - Crossref ER - TY - JOUR TI - A New Monte Carlo Simulation Technique for the Study of Epitaxial Crystal Growth AU - Faux, D. A. AU - Hall, C. K. AU - Bernholc, J. T2 - Molecular Simulation AB - Abstract A new type of Monte Carlo simulation is described which models epitaxial crystal growth on non-lattice-matched substrates. The model allows adatoms to immediately occupy positions of local potential minima which are defined by the particle-particle interactions. Adatoms make discrete hops from one minimum to another. This model therefore combines some of the flexibility of continuous-space Monte Carlo with the speed associated with lattice-like simulations. Details of the Monte Carlo procedure are presented for a two-dimensional system where the particle-particle interactions are assumed to be of the Lennard-Jones type. Some results are presented for the case where the adsorbate crystal lattice is larger than the substrate lattice by 2% and 8%. DA - 1990/5// PY - 1990/5// DO - 10.1080/08927029008022400 VL - 4 IS - 6 SP - 361-369 J2 - Molecular Simulation LA - en OP - SN - 0892-7022 1029-0435 UR - http://dx.doi.org/10.1080/08927029008022400 DB - Crossref KW - Monte Carlo simulation KW - mismatched interfaces KW - crystal growth KW - two-dimensional systems ER - TY - JOUR TI - ON THE ISOLATION OF EMBRYONIC STEM-CELLS - COMPARATIVE BEHAVIOR OF MURINE, PORCINE AND OVINE EMBRYOS AU - PIEDRAHITA, JA AU - ANDERSON, GB AU - BONDURANT, RH T2 - THERIOGENOLOGY AB - The efficiency of isolation and the characteristics of embryo-derived cell lines from murine, porcine, and ovine embryos cultured on STO feeders or homologous embryonic fibroblasts (HEF) feeders were compared. While murine isolated ICM or intact embryos plated on STO or HEF feeders gave rise to cell lines with embryonic stem cell-like (ES-like) morphology, ovine embryos did not. Cell lines with ES-like morphology were isolated from porcine intact embryos and isolated ICM when plated on STO feeders but not when plated on HEF. Neither murine nor porcine ES-like cell lines expressed cytokeratin 18 or vimentin. Unlike murine ES-like cell lines, porcine ES-like cells did not undergo observable differentiation in vitro or in vivo. Cell lines with epithelial-like morphology were isolated from porcine and ovine embryos. Both porcine and ovine epithelial-like cell kines expressed cytokeratin 18. When induced to differentiate in vitro, porcine and ovine epithelial-like cell lines formed vesicular structures. Electron microscopy revealed that the porcine vesicles were composed of polarized epithelial cells, each with a basally-located nucleus and an apical border containing numerous microvilli with a well organized microfilament core. The results of this study show that conditions which allow isolation of ES cells from murine embryos allow the isolation of porcine embryo-derived cell lines sharing some, but not all, the characteristics of murine ES cells. DA - 1990/11// PY - 1990/11// DO - 10.1016/0093-691X(90)90559-C VL - 34 IS - 5 SP - 879-901 SN - 1879-3231 ER - TY - JOUR TI - INFLUENCE OF FEEDER LAYER TYPE ON THE EFFICIENCY OF ISOLATION OF PORCINE EMBRYO-DERIVED CELL-LINES AU - PIEDRAHITA, JA AU - ANDERSON, GB AU - BONDURANT, RH T2 - THERIOGENOLOGY AB - Experiments were conducted to determine the effects of feeder layers composed of different cell types on the efficiency of isolation and the behavior of porcine embryo-derived cell lines. Inner cell masses (ICM) isolated from 7- to 8-d-old embryos were plated on feeder layers composed of Buffalo rat liver cells (BRL), a continuous cell line of murine embryonic fibroblasts (STO), STO combined with BRL at a 9:1 and 1:1 ratio, STO with BRL-conditioned medium (STO + CM), porcine embryonic fibroblasts (PEF), PEF combined with BRL at a 9:1 and 1:1 ratio, porcine uterine epithelial cells (PUE), murine embryonic fibroblasts (MEF), or an epithelial-like porcine embryo-derived cell line (PH3A). It was found that embryo-derived cell lines could be isolated only from the STO and the STO with BRL-conditioned medium treatments. The isolated cell lines were of epithelial-like and embryonic stem cell-like (ES-like) morphology. The feeders tested had an effect on the behavior of plated ICM. Some feeders, represented by PUE, BRL, STO:BRL (1:1), PEF:BRL (1:1), and PH3A, did not promote attachment of the ICM to the feeder layer; others, represented by STO and MEF, allowed attachment, differentiation and proliferation. On PEF feeders the ICM spread onto the feeder layer after attachment without apparent signs of proliferation or differentiation. None of the feeders tested increased the efficiency of isolation or the growth characteristics of embryo-derived (both ES-like and epithelial-like) cell lines over that of STO feeders. DA - 1990/11// PY - 1990/11// DO - 10.1016/0093-691X(90)90558-B VL - 34 IS - 5 SP - 865-877 SN - 1879-3231 ER - TY - JOUR TI - OCULAR INFLAMMATORY EFFECTS OF INTRAVITREALLY INJECTED TUMOR NECROSIS FACTOR-ALPHA AND ENDOTOXIN AU - FLEISHER, LN AU - FERRELL, JB AU - MCGAHAN, MC T2 - INFLAMMATION DA - 1990/6// PY - 1990/6// DO - 10.1007/BF00915816 VL - 14 IS - 3 SP - 325-335 SN - 0360-3997 ER - TY - JOUR TI - COPPER AND ASPIRIN TREATMENT INCREASE THE ANTIOXIDANT ACTIVITY OF PLASMA AU - MCGAHAN, MC T2 - AGENTS AND ACTIONS DA - 1990/8// PY - 1990/8// DO - 10.1007/bf02003222 VL - 31 IS - 1-2 SP - 59-64 SN - 0065-4299 ER - TY - JOUR TI - Pharmacokinetics, inhibition of lymphoblast transformation and subacute oral toxicity of cyclosporine in swine AU - Vaden, S. L. AU - Riviere, J. E. T2 - American Journal of Veterinary Research DA - 1990/// PY - 1990/// VL - 51 SP - 399-403 ER - TY - JOUR TI - Isolated perfused tumored skin flap model AU - Vaden, S. L. AU - Riviere, J. E T2 - Veterinary Cancer Society Newsletter DA - 1990/// PY - 1990/// VL - 14 SP - 1-6 ER - TY - JOUR TI - Serosurvey of shelter dogs in Virginia for antibodies to Borrelia burgdorferi AU - Torrence, Mary E. AU - Jenkins, Suzanne R. AU - Levine, Jay F. AU - Nicholson, William L. AU - Pelzer, Kevin D. T2 - Preventive Veterinary Medicine AB - Increased interest and concern about Lyme disease in the last several years, along with the close proximity of Maryland's endemic foci, has raised questions about the incidence of Lyme disease in Virginia. A canine seroprevalence survey was undertaken in an attempt to estimate the prevalence of borreliosis in Virginia. An ELISA test was used to detect antibodies against Borrelia burgdorferi in sera from 221 shelter dogs from the three different physiographic regions in Virginia. From those animals sampled, only one dog was seropositive. This finding was consistent with other studies which have found limited evidence of Lyme disease in Virginia. DA - 1990/12// PY - 1990/12// DO - 10.1016/0167-5877(90)90049-N VL - 10 IS - 1-2 SP - 41-46 J2 - Preventive Veterinary Medicine LA - en OP - SN - 0167-5877 UR - http://dx.doi.org/10.1016/0167-5877(90)90049-N DB - Crossref ER - TY - JOUR TI - SPECIES DISTRIBUTION OF ULCERATIVE LESIONS ON FINFISH IN THE TAR-PAMLICO RIVER ESTUARY, NORTH-CAROLINA AU - LEVINE, JF AU - HAWKINS, JH AU - DYKSTRA, MJ AU - NOGA, EJ AU - MOYE, DW AU - CONE, RS T2 - DISEASES OF AQUATIC ORGANISMS DA - 1990/3/6/ PY - 1990/3/6/ DO - 10.3354/dao008001 VL - 8 IS - 1 SP - 1-5 SN - 0177-5103 ER - TY - JOUR TI - Cytosolic water-soluble phosphodiester in vertebrate lens AU - Burt, C AU - Levine, J T2 - Biochimica et Biophysica Acta (BBA) - General Subjects AB - The water-soluble phosphate profiles determined by 31P nuclear magnetic resonance (NMR) of a broad range of vertebrate lenses are presented. We identify threonine ethanolamine phosphodiester (TEP) in intact lenses and lens extracts by its chemical shift and its physical and chemical characteristics. Amongst the species studied, we give the first characterization of frog lens where not only serine ethanolamine phosphodiesters (SEP) but TEP are found. DA - 1990/2/26/ PY - 1990/2/26/ DO - 10.1016/0304-4165(90)90011-k VL - 1033 IS - 2 SP - 189-193 J2 - Biochimica et Biophysica Acta (BBA) - General Subjects LA - en OP - SN - 0304-4165 UR - http://dx.doi.org/10.1016/0304-4165(90)90011-k DB - Crossref ER - TY - JOUR TI - ATTENUATION OF BORRELIA-ANSERINA BY SERIAL PASSAGE IN LIQUID-MEDIUM AU - LEVINE, JF AU - DYKSTRA, MJ AU - NICHOLSON, WL AU - WALKER, RL AU - MASSEY, G T2 - RESEARCH IN VETERINARY SCIENCE AB - Borrelia anserina (Sakharoff) was successfully grown in a liquid medium (Barbour-Stoenner-Kelly) for 39 passages. By the 12th serial passage in medium, infectivity of B anserina for chicks was lost. Electron microscopy did not reveal structural differences between non-infective and infective cultured organisms. Changes in the protein profiles were found by electrophoresis as the organisms were passed in culture. DA - 1990/1// PY - 1990/1// DO - 10.1016/s0034-5288(18)31511-x VL - 48 IS - 1 SP - 64-69 SN - 0034-5288 ER - TY - JOUR TI - OXYGEN METABOLITES STIMULATE RELEASE OF HIGH-MOLECULAR-WEIGHT GLYCOCONJUGATES BY CELL AND ORGAN-CULTURES OF RODENT RESPIRATORY EPITHELIUM VIA AN ARACHIDONIC ACID-DEPENDENT MECHANISM AU - ADLER, KB AU - HOLDENSTAUFFER, WJ AU - REPINE, JE T2 - JOURNAL OF CLINICAL INVESTIGATION AB - Several common pulmonary disorders characterized by mucus hypersecretion and airway obstruction may relate to increased levels of inhaled or endogenously generated oxidants (O2 metabolites) in the respiratory tract. We found that O2 metabolites stimulated release of high-molecular-weight glycoconjugates (HMG) by respiratory epithelial cells in vitro through a mechanism involving cyclooxygenase metabolism of arachidonic acid. Noncytolytic concentrations of chemically generated O2 metabolites (purine + xanthine oxidase) stimulated HMG release by cell and explant cultures of rodent airway epithelium, an effect which is inhibitable by coaddition of specific O2 metabolite scavengers or inhibitors of arachidonic acid metabolism. Addition of O2 metabolites to epithelial cells provoked production of PGF2a, an effect also inhibitable by coaddition of O2 metabolite scavengers or inhibitors of arachidonic acid metabolism. Finally, addition of exogenous PGF2a to cell cultures stimulated HMG release. We conclude that O2 metabolites increase release of respiratory HMG through a mechanism involving cyclooxygenase metabolism of arachidonic acid with production mainly of PGF2a. This mechanism may be fundamental to the pathogenesis of a variety of lung diseases associated with hypersecretion of mucus and/or other epithelial fluids, as well as a basic cellular response to increased oxidants. DA - 1990/1// PY - 1990/1// DO - 10.1172/jci114436 VL - 85 IS - 1 SP - 75-85 SN - 0021-9738 ER - TY - JOUR TI - INHIBITION OF LUNG INJURY, INFLAMMATION, AND INTERSTITIAL PULMONARY FIBROSIS BY POLYETHYLENE GLYCOL-CONJUGATED CATALASE IN A RAPID INHALATION MODEL OF ASBESTOSIS AU - MOSSMAN, BT AU - MARSH, JP AU - SESKO, A AU - HILL, S AU - SHATOS, MA AU - DOHERTY, J AU - PETRUSKA, J AU - ADLER, KB AU - HEMENWAY, D AU - MICKEY, R AU - VACEK, P AU - KAGAN, E T2 - AMERICAN REVIEW OF RESPIRATORY DISEASE AB - Several in vitro studies suggest the involvement of active oxygen metabolites in cell damage caused by asbestos. To determine if lung injury, inflammation, and asbestosis could be inhibited in vivo in a rapid-onset, inhalation model of disease, a novel method of chronic administration of antioxidant enzymes was developed. In brief, Fischer 344 rats were treated with polyethylene glycol-conjugated (PEG-) superoxide dismutase or catalase in osmotic pumps over a 10-day (5 days/wk for 2 wk) or 20-day (5 days/wk for 2 wk) period of exposure to crocidolite asbestos. Control rats included sham-exposed animals and those exposed to asbestos but receiving chemically inactivated enzymes. After 10 days of exposure to asbestos, lactic dehydrogenase (LDH), alkaline phosphatase, and total protein in bronchoalveolar lavage (BAL) were measured in one group of rats. Total and differential cell counts in BAL also were assessed. After 20 days of exposure, lungs of an additional group of rats were evaluated by histopathology and by measurement of hydroxyproline. Asbestos-associated elevations in LDH, protein, and total cell numbers in BAL were reduced in rats receiving PEG-catalase. Decreases in numbers of alveolar macrophages, polymorphonuclear leukocytes, and lymphocytes occurred in these animals. Exposure to asbestos for 20 days caused significant increases in both the amount of hydroxyproline in lung and the severity and extent of fibrotic lesions as determined by histopathology. These indicators of asbestosis were inhibited in a dosage-dependent fashion in rats receiving PEG-catalase. Use of inactivated PEG-catalase failed to boost serum levels of catalase and did not inhibit asbestos-induced elevation of hydroxyproline in lung. These results confirm the importance of active oxygen species in asbestos-associated lung injury and suggest the possible future use of a therapeutic approach to clinical asbestosis. DA - 1990/5// PY - 1990/5// DO - 10.1164/ajrccm/141.5_Pt_1.1266 VL - 141 IS - 5 SP - 1266-1271 SN - 0003-0805 ER - TY - CHAP TI - Contractile cells in normal and fibrotic lungs. AU - Adler, K. B. AU - Low, R. B. AU - Leslie, K. O. AU - Mitchell, J. AU - Evans, J. N. T2 - Pathology reviews. AB - Interstitial pulmonary fibrosis represents a group of fibrosing lung diseases, often of unknown etiology, characterized by hypercellularity and deposition of connective tissue within the interstitium of the alveolar wall (32, 65). Increased “stiffness” of the lung leads to reduced lung volumes and lowered dynamic compliance (80). In interstitial fibrosis, as well as several other forms of lung injury, there is an apparent increase in smooth muscle (SM) cells organized into bundles within areas of the lung where these cells normally are present but difficult to detect, such as the alveolar duct (69, 89, 119). This increase in acinar SM can be quite prominent, and, in some cases, the degree of parenchymal SM hyperplasia is sufficiently excessive to be referred to as “muscular cirrhosis of the lung” (2, 7, 33, 110, 152). PY - 1990/// DO - 10.1007/978-1-4612-0485-5_3 SP - 25-37 ER - TY - JOUR TI - CHARACTERIZATION OF GUINEA-PIG TRACHEAL EPITHELIAL-CELLS MAINTAINED IN BIPHASIC ORGANOTYPIC CULTURE - CELLULAR COMPOSITION AND BIOCHEMICAL-ANALYSIS OF RELEASED GLYCOCONJUGATES AU - ADLER, KB AU - CHENG, PW AU - KIM, KC T2 - AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY AB - An air-liquid interface (biphasic) primary culture system in which guinea pig tracheal epithelial cells maintain morphologic characteristics of differentiated epithelium has been developed in this laboratory. In this report, we compared quantitatively cell populations of 8-day cultures to those of epithelial mucosa in intact trachea. In addition, high molecular weight glycoconjugates released by the cultured cells were isolated and characterized. Quantitative morphometric analysis revealed similar volume densities of ciliated, secretory, basal, and “other” cells in cultures and in intact tracheal surface epithelium, although the cultures tended to have smaller cells and contained fewer basal cells. High molecular weight glycoconjugates released apically by cell cultures and excluded from Sepharose CL-4B columns contained approximately 5% hyaluronic acid but undetectable amounts of other proteoglycans, such as chondroitin sulfate, heparan sulfate, and dermatan sulfate. The hyaluronidase-resistant glycoconjugates exhibited a peak buoyant density at 1.49 g/ml on cesium chloride density gradient centrifugation and were shown to contain mucin-type carbohydrate to peptide linkages (i.e., GalNAc to ser/thr) and an amino acid composition typical of respiratory mucins. The results indicate that this organotypic cell culture system mimics quite closely morphology of mucosal epithelium in intact airways and that the cells release high molecular weight glycoconjugates with biochemical properties of mucin-type glycoproteins. Thus, this in vitro system appears well-suited for studies of mucin secretion and other functions of respiratory epithelial cells. DA - 1990/2// PY - 1990/2// DO - 10.1165/ajrcmb/2.2.145 VL - 2 IS - 2 SP - 145-154 SN - 1044-1549 ER - TY - JOUR TI - Alveolar Type II cell response in rats exposed to aerosols of alpha cristobalite. AU - Low, R. B. AU - Leslie, K. O. AU - Hemenway, D. R. AU - Absher, P. M. AU - Alder, K. B. AU - Giancola, M. S. AU - Vacek, P. M. T2 - American Journal of Pathology DA - 1990/// PY - 1990/// VL - 136 SP - 923-931 ER - TY - JOUR TI - Specific interactions with extracellular matrix may influence epithelial repair mechanisms in the pulmonary alveolus. AU - Sannes, P. AU - Peters, B. AU - Adler, K. T2 - Proceedings of the 30th Aspen Lung Conference. DA - 1990/// PY - 1990/// VL - 30 SP - 24 ER - TY - JOUR TI - Oxygen metabolites stimulate inositol lipid turnover in guinea pig airway epithelial cells in organotypic culture. AU - Adler, K. B. AU - Akley, N. J. AU - Lee, J. T2 - American Review of Respiratory Disease DA - 1990/// PY - 1990/// VL - 141 SP - A107 ER - TY - JOUR TI - Interactions between extracellular matrix, airway and alveolar epithelium: alterations in fibrosis and possible pathogenetic significance. AU - Adler, K. B. AU - Peters, B. P. AU - Krunkosky, T. AU - Sannes, P. L. T2 - Proceedings of the Sixth International Colloquium Pulmonary Fibrosis. DA - 1990/// PY - 1990/// VL - 6 SP - 39 ER - TY - JOUR TI - PATHOLOGICAL AND MICROBIOLOGICAL FEATURES OF AEROMONAS-SALMONICIDA INFECTION IN THE AMERICAN EEL (ANGUILLA-ROSTRATA) AU - NOGA, EJ AU - BERKHOFF, HA T2 - FISH PATHOLOGY AB - An ulcerative skin disease which is very similar clinically to that reported in Japanese eel (Anguilla japonica) has caused morbidity in both wild and cultured populations of American eel (A. rostrata) in North Carolina, USA. Lesions appear to begin as localized, depigmented foci which spread to form large patches of necrotic skin up to 16 cm2 in area. The depigmented patches detach at the dermo-epidermal junction, forming large ulcers that expose underlying muscle. The infection commonly affects the head, producing cranial swelling and corneal edema. A mild to severe, primarily mononuclear infiltrate is seen, most prominently in large ulcers. Many lesions had extensive collagen deposition, which contributed to the tissue swelling. Culture of skin lesions in various stages of development revealed the consistent presence of bacterial isolates that were biochemically and immunologically identified as Aeromonas salmonicida. This agent was the predominant organism by colony type in 17 of 20 skin cultures and usually appeared to constitute over 90% of the colonies present. Kidney samples were free of infection. DA - 1990/9// PY - 1990/9// DO - 10.3147/jsfp.25.127 VL - 25 IS - 3 SP - 127-132 SN - 0388-788X ER - TY - JOUR TI - Epidemiology of ulcerative mycosis in Atlantic menhaden in the Tar-Pamlico River estuary, North Carolina AU - Levine, Jay AU - Hawkins, J. H. AU - Dykstra, M. J. AU - Noga, E. J. AU - Moye, D. W. AU - Cone, R. S. T2 - Journal of Aquatic Animal Health AB - Ulcerative mycosis was observed on Atlantic menhaden Brevoortia tyrannus collected in pound-net and trawl samples obtained from the Tar-Pamlico River estuary, North Carolina, during May 1985-April 1987 and in cast-net samples obtained during October-November 1986. The disease was most prevalent on Atlantic menhaden caught in pound nets during November 1986 and January 1987, and in trawls during May 1986 and November 1986. Disease was also most prevalent among yearlings in the spring and was probably acquired in the estuary. DA - 1990/// PY - 1990/// DO - 10.1577/1548-8667(1990)002<0162:eoumia>2.3.co;2 VL - 2 IS - 3 SP - 162 ER - TY - JOUR TI - EPIDEMIC ORAL ULCERATION IN LARGEMOUTH BASS (MICROPTERUS-SALMOIDES) ASSOCIATED WITH THE LEECH MYZOBDELLA-LUGUBRIS AU - NOGA, EJ AU - BULLIS, RA AU - MILLER, GC T2 - JOURNAL OF WILDLIFE DISEASES AB - An epidemic of severe ulcerations of the tongue and buccal cavity was documented in subadult and adult largemouth bass (Micropterus salmoides) from Currituck Sound, North Carolina (USA). Other external clinical signs were not present. Between November 1986 and May 1987, reports from fishermen indicated that as many as 90% of large (greater than 300 mm total length) bass were affected. Older fish were the most commonly involved. The leech, Myzobdella lugubris (= Illinobdella moorei), was consistently present on or near the lesions. Lesions were heavily infected with several different bacteria that were apparently secondary invaders. Stressful (high) salinity or an interruption in the normal migratory cycle of the parasite are suggested as possible causes for this condition in these bass. DA - 1990/1// PY - 1990/1// DO - 10.7589/0090-3558-26.1.132 VL - 26 IS - 1 SP - 132-134 SN - 1943-3700 ER - TY - JOUR TI - ATTEMPTED EHRLICHIA-RISTICII TRANSMISSION WITH DERMACENTOR-VARIABILIS (ACARI, IXODIDAE) AU - LEVINE, JF AU - LEVY, MG AU - NICHOLSON, WL AU - GAGER, RB T2 - JOURNAL OF MEDICAL ENTOMOLOGY AB - Larval Dermacentor variabilis (Say) (n = 327) were fed on Balb/C mice inoculated with Ehrlichia risticii, the etiologic agent of equine monocytic ehrlichiosis (Potomac horse fever). All mice displayed clinical signs of E. risticii infection at the time of feeding. After molting, resulting nymphs (n = 74) were fed on susceptible mice. No clinical signs were observed, and the mice remained seronegative for 6 wk after feeding. DA - 1990/9// PY - 1990/9// DO - 10.1093/jmedent/27.5.931 VL - 27 IS - 5 SP - 931-933 SN - 1938-2928 ER - TY - JOUR TI - RATIONAL DESIGN AND SYNTHESIS OF POLARIZED KETONES AS INHIBITORS OF JUVENILE-HORMONE ESTERASE - IMPORTANCE OF JUVENILE-HORMONE MIMICRY AU - ROE, RM AU - LINDERMAN, RJ AU - LONIKAR, M AU - VENKATESH, K AU - ABDELAAL, YAI AU - LEAZER, J AU - UPCHURCH, L T2 - JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY AB - ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRational design and synthesis of polarized ketones as inhibitors of juvenile hormone esterase: importance of juvenile hormone mimicryR. Michael Roe, Russell J. Linderman, Madhu Lonikar, Krishnappa Venkatesh, Yehia A. I. Abdel-Aal, Johnnie Leazer, and Lisa UpchurchCite this: J. Agric. Food Chem. 1990, 38, 5, 1274–1278Publication Date (Print):May 1, 1990Publication History Published online1 May 2002Published inissue 1 May 1990https://doi.org/10.1021/jf00095a027RIGHTS & PERMISSIONSArticle Views76Altmetric-Citations14LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (656 KB) Get e-Alertsclose Get e-Alerts DA - 1990/5// PY - 1990/5// DO - 10.1021/jf00095a027 VL - 38 IS - 5 SP - 1274-1278 SN - 0021-8561 ER - TY - JOUR TI - HUMAN RECOMBINANT INTERLEUKIN-2 INDUCES MATURATION AND ACTIVATION SIGNALS FOR FELINE EOSINOPHILS INVIVO AU - TOMPKINS, MB AU - NOVOTNEY, C AU - GRINDEM, CB AU - PAGE, R AU - ENGLISH, R AU - NELSON, P AU - TOMPKINS, WAF T2 - JOURNAL OF LEUKOCYTE BIOLOGY AB - Abstract Immunotherapy, with interleukin-2 (IL-2) or IL-2 plus lymphokine-activated killer (LAK) cells, has been used to treat cancer and acquired immunodeficiency syndrome (AIDS) in man. Similarities between feline leukemia virus (FeLV) infection in the cat and human immunodeficiency virus (HIV) infection in man have prompted immunotherapeutic studies in the cat. To develop baseline data on hematological responses to infused IL-2, cats were given daily (1–14 days) i.v. injections of 5 × 104 U/kg of recombinant human IL-2 (rHulL-2). Complete blood cell (CBC) counts were done weekly. Red blood cell (RBC), neutrophil, and lymphocyte numbers did not change appreciably over the course of the study. In contrast, rHulL-2 caused an eosinophilia in all but the 1 day treatment group. Treatment for 3 days generated a transient eosinophilia on day 7 that returned to baseline by 3 weeks. Five day and 7 day treatments generated an eosinophilia by day 7 that peaked on day 14 and returned to normal values by day 28. Treatment of cats for 14 days did not increase the magnitude or duration of the eosinophilia beyond the 5 or 7 day treatments. Bone marrow (BM) biopsies from rHulL-2-treated cats revealed a marked selective hyperplasia of eosinophil precursors. In the 5 day treatment group, all maturation stages of eosinophils were elevated by week 1 of treatment. By week 2, the early stages had returned to normal, whereas the late stage cells remained elevated, suggesting an ordered maturation response. Numbers of all eosinophil precursors approximated pretreatment numbers by weeks 3–4. Thus the BM hyperplasia preceded the blood eosinophilia by 1 week, suggesting that an enhanced maturation response of BM eosinophil precursors is a major contributor to the rHulL-2-induced blood eosinophilia. In addition to a maturation signal, rHulL-2 induces a potent activation signal for eosinophils as measured by a decrease in density and an increase in longevity in culture. The significance of the activated eosinophil in the therapeutic or toxicologic response to rHulL-2 infusion is discussed. DA - 1990/12// PY - 1990/12// DO - 10.1002/jlb.48.6.531 VL - 48 IS - 6 SP - 531-540 SN - 0741-5400 ER - TY - JOUR TI - CHARACTERIZATION OF MONOCLONAL-ANTIBODIES TO FELINE LYMPHOCYTES-T AND THEIR USE IN THE ANALYSIS OF LYMPHOCYTE TISSUE DISTRIBUTION IN THE CAT AU - TOMPKINS, MB AU - GEBHARD, DH AU - BINGHAM, HR AU - HAMILTON, MJ AU - DAVIS, WC AU - TOMPKINS, WAF T2 - VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY AB - We describe the development of three monoclonal antibodies to feline T lymphocytes. Antibody 1.572 stains 93% of feline thymocytes, 49% of lymph node, and 65% of spleen lymphocytes. Two-color analysis shows 1.572 does not stain Ig-bearing cells, and 1.572-positive lymphocytes plus Ig-positive lymphocytes make up approximately 90% of peripheral blood lymphocytes (PBL), suggesting that 1.572 is a pan-T cell marker. The other two monoclonal antibodies, 3.357 and CAT30A, stain a smaller population of thymocytes (59%) of which 40% express both antigens. The 3.357 antigen is found on 23% of lymph node and 47% of spleen lymphocytes, while the CAT30A antigen is found on 29% of lymph node and 19% of spleen lymphocytes. Two-color analysis shows that 3.357 and CAT30A stain mutually exclusive subpopulations of 1.572-positive cells. Using thymocytes as an antigen source, antibody 3.357 precipitated a molecule of 66,000 molecular weight (Mw) under nonreducing conditions and a heterodimer of 32,000 and 34,000 under reducing conditions, suggesting that 3.357 recognizes the feline CD8 homologue. Antibody CAT30A precipitated a molecule of 55,000 Mw under both reducing and nonreducing conditions, which suggests it recognizes the feline CD4 homologue. Analysis of PBL profiles of 35 normal cats using the three monoclonal antibodies indicates that the distribution of feline PBL subpopulations is similar to man, including the CAT30A:3.357 ratio (1.74), which is identical to reported CD4:CD8 ratios in man. Based on these data, the feline CD4 and CD8 homologues are similar to those reported in other species. DA - 1990/12// PY - 1990/12// DO - 10.1016/0165-2427(90)90115-9 VL - 26 IS - 4 SP - 305-317 SN - 0165-2427 ER - TY - JOUR TI - A TOMATO SPOTTED WILT-LIKE VIRUS WITH A SEROLOGICALLY DISTINCT N-PROTEIN AU - LAW, MD AU - MOYER, JW T2 - JOURNAL OF GENERAL VIROLOGY AB - A serologically distinct member of the tomato spotted wilt virus (TSWV) group was isolated from the hybrid flower crop New Guinea impatiens (Impatiens sp.) and termed TSWV-I. TSVW-I type isolates have frequently been detected in a wide variety of flower crops throughout the United States. TSWV-shares many characteristics with TSWV, such as symptomatology and possession of three ssRNA species (L, M and S of 8·3 kb, 5·2 kb and 3·4 kb, respectively) and three structural proteins (G1, G2 and N of 78K, 52K and 28K respectively). The TSWV-I G1 and G2 glycoproteins were serologically related to the respective proteins of TSWV, but the TSWV-I nucleocapsid or N protein was serologically unrelated to that of TSWV. Hybridization analysis under high stringency conditions revealed no hybridization between clones of TSWV-1 S and M and the S and M RNAs of TSWV, respectively and in addition, a TSWV S clone hybridized only with TSWV S RNA. The cytopathology of TSWV-1 also differed from that of TSWV. TSWV-1- infected tissue primarily contained filamentous structures arranged in paracrystalline arrays, which were also observed by immunosorbent electron microscopy of tissue extracts. The filamentous structures were only trapped by TSWV-1 antibodies. The conserved serological relatedness between TSWV types for G1 and G2, but not N, is consistent with serological analyses of the nairovirus and phlebovirus genera of the Bunyaviridae, the virus family that TSWV most closely resembles. DA - 1990/4// PY - 1990/4// DO - 10.1099/0022-1317-71-4-933 VL - 71 SP - 933-938 SN - 0022-1317 ER - TY - JOUR TI - Glucose, glutamine and inorganic phosphate in early development of the pig embryo in vitro AU - Petters, R. M. AU - Johnson, B. H. AU - Reed, M. L. AU - Archibong, A. E. T2 - Journal of Reproduction & Fertility AB - Summary. Pig embryos at the 1- or 2-cell stage (before the 'block' to development in vitro) were cultured in 8 different media derived from Krebs'–Ringer–bicarbonate medium. A 2 × 2 × 2 factorial arrangement was used for the treatments, with glucose, glutamine and phosphate being the major effects tested. Embryos were obtained from sows approximately 44–48 h after the observation of oestrus, with the majority being at the 1-cell stage. Embryos from each female were randomly assigned to each treatment. After in-vitro culture, all embryos were scored for the stage of development attained and stained to determine final cell number. Significant effects were evident due to female, glucose, glutamine, a phosphate × glucose interaction and a glutamine × glucose interaction. None of the media components tested was inhibitory to embryo development. The greatest development (45–60% morula or blastocyst) was achieved with glucose and glutamine (both alone and in combination) in the media, demonstrating that an amino acid can serve as the sole energy source for complete preimplantation embryonic development in vitro. Keywords: embryo; pig; in vitro culture; glutamine; glucose; phosphate DA - 1990/// PY - 1990/// DO - 10.1530/jrf.0.0890269 VL - 89 IS - 1 SP - 269 ER - TY - JOUR TI - Glomerulonephritis AU - Vaden, S. L. T2 - Kal Kan Forum DA - 1990/// PY - 1990/// VL - 9 IS - 4 SP - 3 ER - TY - JOUR TI - Distribution of equine infectious anemia in equids in southeastern United States AU - Loftin, M. K. AU - Levine, J. F. AU - McGinn, T. AU - Coggins, L. T2 - Journal of the American Veterinary Medical Association DA - 1990/// PY - 1990/// VL - 197 IS - 8 SP - 1018-120 ER - TY - JOUR TI - VIRULENCE OF INFECTIOUS LARYNGOTRACHEITIS VIRUSES - COMPARISON OF MODIFIED-LIVE VACCINE VIRUSES AND NORTH-CAROLINA FIELD ISOLATES AU - GUY, JS AU - BARNES, HJ AU - MORGAN, LM T2 - AVIAN DISEASES AB - Virulence of six modified-live (ML) infectious laryngotracheitis (ILT) vaccine viruses was compared with that of 11 field isolates (indistinguishable from vaccine viruses by DNA restriction endonuclease analyses) by intratracheal exposure of 4-week-old, specific-pathogen-free chickens. Virulence of ILT viruses was based on an intratracheal pathogenicity index, mortality, and tracheal lesions. Intratracheal pathogenicity indices for ML vaccine viruses ranged from 0.0 to 0.14, while those for field isolates were 0.20 to 0.82. Mortality was a consistent clinical feature of field isolates; all produced mortality, with seven of the 11 isolates causing two or more deaths per inoculation group. In contrast, only one of six ML vaccine viruses produced mortality (one death per inoculation group). In general, tracheal lesions were more severe in chickens inoculated with field isolates and were produced more consistently than in chickens inoculated with vaccine viruses. These studies indicate that virulence of ILT field isolates was greater than that of ML vaccine viruses. Together with previous restriction endonuclease analyses, these findings suggest the possibility that field isolates originated from ML vaccine viruses through reversion to parental-type virulence. DA - 1990/// PY - 1990/// DO - 10.2307/1591340 VL - 34 IS - 1 SP - 106-113 SN - 0005-2086 ER - TY - JOUR TI - Tick-borne zoonoses AU - Breitschwerdt, E. B. T2 - Veterinary Technician DA - 1990/// PY - 1990/// VL - 11 IS - 4 SP - 249 ER - TY - JOUR TI - SUBTOTAL CANINE PROSTATECTOMY WITH THE NEODYMIUM - YTTRIUM-ALUMINUM-GARNET LASER AU - HARDIE, EM AU - STONE, EA AU - SPAULDING, KA AU - CULLEN, JM T2 - VETERINARY SURGERY AB - A technique was developed for subtotal prostatectomy in dogs with the neodymium:yttrium‐aluminum‐garnet (Nd:YAG) laser. In six normal dogs, full‐thickness necrosis of the prostate occurred if the central‐lateral region within 5 mm of the urethra was photoablated at 60 watts for 1 second. Moderate to superficial necrosis occurred when the prostate within 5 mm of the urethra was photoablated at 35 watts for 2 seconds or 60 watts for 0.5 second. At necropsy, leakage of the urethra occurred in two dogs at sites treated at 60 watts for 1 second. In a clinical study, complications associated with subtotal prostatectomy with the Nd:YAG laser (n‐6) were compared with complications associated with prostatic drainage (n = 6) in dogs with prostatic disease. Intraoperative death (2/6 dogs) and nocturnal incontinence (4/4 surviving dogs) occurred with subtotal prostatectomy. Uncontrolled prostatic infection (2/6 dogs) occurred with prostatic drainage and resulted in the death of one dog on day 11. Four of five dogs surviving prostatic drainage developed recurrent urinary tract infection. DA - 1990/// PY - 1990/// DO - 10.1111/j.1532-950X.1990.tb01205.x VL - 19 IS - 5 SP - 348-355 SN - 0161-3499 ER - TY - JOUR TI - Pharmacokinetics of flunixin meglumine in lactating cattle after single and multiple intramuscular and intravenous administrations AU - Anderson, K. L. AU - Neff-Davis, C. A. AU - Davis, L. E. AU - Bass, V. D. T2 - American Journal of Veterinary Research DA - 1990/// PY - 1990/// VL - 51 IS - 9 SP - 1464 ER - TY - JOUR TI - Microbiological analysis of bulk-tank milks on blood agar: Comparison with regulatory methods and influence of sample collection and handling factors AU - Anderson, K. L. AU - Walker, R. L. AU - Wesen, D. P. T2 - Dairy, Food and Environmental Sanitation DA - 1990/// PY - 1990/// VL - 10 IS - 4 SP - 213 ER - TY - JOUR TI - Kinetics of IgM and IgG responses to experimental and naturally required Rickettsia rickettsii infection in dogs AU - Breitschwerdt, E. B. AU - Levy, M. G. AU - Davidson, M. G. AU - Walker, D. H. AU - Burgdorfer, W. AU - Curtis, B. C. AU - Babineau, C. A. T2 - American Journal of Veterinary Research DA - 1990/// PY - 1990/// VL - 51 IS - 8 SP - 1312 ER - TY - JOUR TI - Geographic Occurrence of Ixodes scapularis and Amblyomma americanum (Acari: Ixodidae) Infesting White-tailed Deer in North Carolina AU - Apperson, Charles S. AU - Levine, Jay F. AU - Nicholson, William L. T2 - Journal of Wildlife Diseases AB - A state-wide survey to determine the occurrence and comparative numbers of ticks infecting white-tailed deer (Odocoileus virginianus) was conducted in North Carolina (USA). One thousand six hundred twenty nine deer were examined in 60 of 100 counties; with the exception of one county in the piedmont region, all tick-infested deer occurred in the coastal plain. Ixodes scapularis (46%) and Amblyomma americanum (53%) were the most prevalent species encountered and accounted for more than 98% of the 4,286 ticks collected. Some specimens of Dermacentor albipictus and Amblyomma maculatum also were collected. DA - 1990/10// PY - 1990/10// DO - 10.7589/0090-3558-26.4.550 VL - 26 IS - 4 SP - 550-553 J2 - Journal of Wildlife Diseases LA - en OP - SN - 0090-3558 UR - http://dx.doi.org/10.7589/0090-3558-26.4.550 DB - Crossref ER - TY - JOUR TI - ENDOTOXIC-SHOCK .2. A REVIEW OF TREATMENT AU - HARDIE, EM AU - KRUSEELLIOTT, K T2 - JOURNAL OF VETERINARY INTERNAL MEDICINE AB - Treatment of endotoxemia is difficult because of the numerous mediators involved in the body's response to endotoxin. There are three possible approaches in treating endotoxemia. The interaction of endotoxin with target cells can be blocked by inducing tolerance, decreasing plasma endotoxin concentrations, or interfering with endotoxin binding. Once endotoxin has interacted with target cells, endogenous mediators can be blocked with a huge variety of drugs. The effects of corticosteroids, cyclooxy‐genase blockers, leukotriene blockers, platelet activating factor blockers, tumor necrosis factor blockers, oxygen radical scavengers, opiate antagonists, antihistamines, calcium channel blockers are detailed. Supportive care of the endotoxemic patient continues to be a critical aspect of treatment. Controversies regarding solutions to use for volume support, vasoactive and cardiostimulant drugs, metabolic support, and treatment of disseminated intravascular coagulation are reviewed. DA - 1990/// PY - 1990/// DO - 10.1111/j.1939-1676.1990.tb03128.x VL - 4 IS - 6 SP - 306-314 SN - 0891-6640 ER - TY - JOUR TI - ENDOTOXIC-SHOCK .1. A REVIEW OF CAUSES AU - HARDIE, EM AU - KRUSEELLIOTT, K T2 - JOURNAL OF VETERINARY INTERNAL MEDICINE AB - Endotoxic shock is a complex phenomenon resulting from systemic release of inflammatory mediators. Endotoxin interacts with inflammatory cells, platelets, and vascular endothelium. Cytokines, such as tumor necrosis factor and interleukins, and lipid mediators (platelet activating factor, thromboxane, prostacyclin, leukotrienes) are released. These primary mediators act synergistically to cause many of the harmful effects associated with endotoxemia. Multiple secondary mediators are released in response to the primary mediators, compounding the damage. The end result is the species-specific clinical syndrome recognized as endotoxemia. DA - 1990/// PY - 1990/// DO - 10.1111/j.1939-1676.1990.tb03119.x VL - 4 IS - 5 SP - 258-266 SN - 0891-6640 ER - TY - CHAP TI - Ecological genetics and integrated pest management AU - Gould, F. T2 - Agroecology A2 - C. R. Carroll, J. H. Vandermeer A2 - Rosset, P. PY - 1990/// SP - 441 PB - New York : McGraw Hill SN - 007052923X ER - TY - JOUR TI - Hepatic neoplasms in aflatoxin B1-treated, congenital duck hepatitis B virus-infected, and virus-free Pekin ducks AU - Cullen, J. M. AU - Marion, P. L. AU - Sherman, G. J. AU - Hong, X. AU - Newbold, J. E. T2 - Cancer Research DA - 1990/// PY - 1990/// VL - 50 IS - 13 SP - 4072 ER - TY - JOUR TI - Differential down-regulation of epidermal protein kinase C (PKC) by tetradecanoylphorbol-13-acetate and diacylglycerol: Association with epidermal hyperplasia and tumor promotion AU - Hansen, L. A. AU - Monteiro-Riviere, N. A. AU - Smart, R. C. T2 - Cancer Research DA - 1990/// PY - 1990/// VL - 50 SP - 5740–5745 ER -