Works Published in 2003
Displaying works 81 - 100 of 401 in total
Sorted by most recent date added to the index first, which may not be the same as publication date order.
2003 journal article
Enzymatic strategies for the characterization of nucleic acids by electrospray ionization mass spectrometry
Rapid Communications in Mass Spectrometry, 17(24), 2699–2706.
MeSH headings : Exodeoxyribonucleases / chemistry; Isotope Labeling / methods; Nucleic Acids / analysis; Nucleic Acids / chemistry; Oligonucleotides / analysis; Oligonucleotides / chemistry; Polymerase Chain Reaction / methods; Polymorphism, Single Nucleotide; Reproducibility of Results; Restriction Mapping / methods; Sensitivity and Specificity; Sequence Alignment / methods; Sequence Analysis, DNA / methods; Spectrometry, Mass, Electrospray Ionization / methods; Viral Proteins
TL;DR:
This work describes an eclectic array of enzymatic strategies invoked in order to detect single-nucleotide polymorphisms by ESI-MS, although other applications may be envisioned.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 27, 2021
2003 journal article
1H NMR and electrospray mass spectrometry of the mono-ionized bis(2,2′-bis(4,5-dimethylimidazole)chloronitrosylruthenium(II) complex ([Ru(NO)Cl(LH2)2]+, LH2=2,2′-bis(4,5-dimethylimidazole)
Inorganica Chimica Acta, 343, 317–328.
author keywords: ruthenium complexes; nitrosyl complexes; imidazole complexes; electrospray mass spectrometry
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Crossref, ORCID
Added: February 27, 2021
2003 journal article
Characterization of the Major Antigenic Protein 2 of Ehrlichia canis and Ehrlichia chaffeensis and Its Application for Serodiagnosis of Ehrlichiosis
Clinical and Diagnostic Laboratory Immunology, 10(4), 520–524.
MeSH headings : Amino Acid Sequence; Animals; Antigens, Bacterial / genetics; Antigens, Bacterial / immunology; Arkansas / epidemiology; Bacterial Proteins; Cross Reactions; Disease Reservoirs; Dog Diseases / diagnosis; Dog Diseases / immunology; Dogs; Ehrlichia canis / genetics; Ehrlichia canis / immunology; Ehrlichia chaffeensis / genetics; Ehrlichia chaffeensis / immunology; Ehrlichiosis / diagnosis; Ehrlichiosis / epidemiology; Ehrlichiosis / immunology; Ehrlichiosis / veterinary; Enzyme-Linked Immunosorbent Assay; Florida / epidemiology; Genes, Bacterial; Humans; Molecular Sequence Data; North Carolina / epidemiology; Recombinant Fusion Proteins / immunology; Sequence Alignment; Sequence Homology, Amino Acid; Serologic Tests / methods; Species Specificity; Virginia / epidemiology
TL;DR:
Genomic Southern blot analysis using digoxigenin-labeled species-specific probes suggested that map2 is a single-copy gene in both Ehrlichia species, but ELISA and Western blot analysis confirmed that the E. chaffeensis rMAP2 failed to serologically differentiate between E. canis and E. Chaffeensis infections.
(via Semantic Scholar)
cvi.asm.org (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
15. Life on Land
(OpenAlex)
Sources: Crossref, ORCID
Added: February 27, 2021
2003 conference paper
Vesicular Volume, the Fusion Pore and Potential ‘Presynaptic’ Mechanisms Modulating Neurotransmitter Release
In J. Kehr, K. Fuxe, U. Ungerstedt, & T. Svensson (Eds.), Monitoring molecules in neuroscience : proceedings of the 10th International Conference on In Vivo Methods : Karolinska Institutet, Stockholm, Sweden June 24-27, 2003 (pp. 189–191). Stockholm, Sweden: Karolinska University Press.
Ed(s): J. Kehr, K. Fuxe, U. Ungerstedt & T. Svensson
Event: 10th International Conference on In Vivo Methods at Stockholm, Sweden on June 24-27, 2003
Source: NC State University Libraries
Added: February 12, 2021
2003 conference paper
Amperometric Detection of Exocytosis at Cells and Artificial Cells
In J. Kehr, K. Fuxe, U. Ungerstedt, & T. Svensson (Eds.), Monitoring molecules in neuroscience : proceedings of the 10th International Conference on In Vivo Methods : Karolinska Institutet, Stockholm, Sweden June 24-27, 2003 (pp. 171–173). Stockholm, Sweden: Karolinska University Press.
Ed(s): J. Kehr, K. Fuxe, U. Ungerstedt & T. Svensson
Event: 10th International Conference on In Vivo Methods at Stockholm, Sweden on June 24-27, 2003
Source: NC State University Libraries
Added: February 12, 2021
2003 journal article
Naturally processed measles virus peptide eluted from class II HLA-DRB1*03 recognized by T lymphocytes from human blood
Virology, 312(2), 495–506.
author keywords: measles-derived peptide; HLA-DRB1*03; measles virus; mass spectrometry; lymphocyte proliferation
MeSH headings : Adolescent; Amino Acid Sequence; Antigen Presentation; Antigens, Viral / analysis; Antigens, Viral / chemistry; Antigens, Viral / immunology; Antigens, Viral / metabolism; Child; Female; HLA-DR Antigens / immunology; HLA-DR Antigens / metabolism; HLA-DRB1 Chains; Humans; Male; Mass Spectrometry; Measles Vaccine / immunology; Measles virus / chemistry; Measles virus / metabolism; Molecular Sequence Data; Peptides / analysis; Peptides / chemistry; Peptides / immunology; Peptides / metabolism; T-Lymphocytes / immunology
TL;DR:
This data provides direct evidence that the antigenic peptide of measles virus was processed by antigen-presenting cells, presented in the context of HLA class II molecules, and was recognized by peripheral blood T cells from healthy individuals previously immunized with measles vaccine.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, ORCID
Added: February 12, 2021
2003 journal article
Coordination-Driven Self-Assembly of Supramolecular Cages: Heteroatom-Containing and Complementary Trigonal Prisms
Journal of the American Chemical Society, 125(32), 9647–9652.
MeSH headings : Anthracenes / chemical synthesis; Anthracenes / chemistry; Benzene Derivatives / chemical synthesis; Benzene Derivatives / chemistry; Cross-Linking Reagents / chemical synthesis; Cross-Linking Reagents / chemistry; Crystallography, X-Ray; Fourier Analysis; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Structure; Organophosphorus Compounds / chemical synthesis; Organophosphorus Compounds / chemistry; Organosilicon Compounds / chemical synthesis; Organosilicon Compounds / chemistry; Spectrometry, Mass, Electrospray Ionization
TL;DR:
The self-assembly of three nanoscopic prisms of approximate size 1 x 4 nm is reported, which completes a pair of nanoscopic complementary 3-D structures.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2003 journal article
Dual Electrospray Ionization Source for Confident Generation of Accurate Mass Tags Using Liquid Chromatography Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
Analytical Chemistry, 75(14), 3411–3418.
MeSH headings : Amino Acid Sequence; Apoproteins / chemistry; Chromatography, Liquid; Cyclotrons; Mass Spectrometry; Molecular Sequence Data; Myoglobin / chemistry; Proteins / chemistry; Proteomics / instrumentation; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectroscopy, Fourier Transform Infrared / instrumentation
TL;DR:
It is argued that the most effective way to account for space-charge effects is to introduce an internal mass calibrant (IMC) using a dual electrospray ionization (ESI) source where the IMC is added from a separate ESI emitter.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2003 journal article
Determination of a correction to improve mass measurement accuracy of isotopically unresolved polymerase chain reaction amplicons by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry
Rapid Communications in Mass Spectrometry, 17(15), 1714–1722.
MeSH headings : Humans; Molecular Weight; Nucleic Acids / analysis; Polymerase Chain Reaction; Proteins / analysis; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization / instrumentation; Spectrometry, Mass, Electrospray Ionization / methods; Spectroscopy, Fourier Transform Infrared / instrumentation; Spectroscopy, Fourier Transform Infrared / methods; Tandem Repeat Sequences / genetics
TL;DR:
A practical correction factor is developed that is applied to the ToC measurement to largely remove the systematic bias introduced by nature and accurately predicts resolving power achievable for Fourier transform ion cyclotron resonance (FT-ICR) mass analysis of large molecules.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2003 journal article
Mass spectral determination of skeletal/cardiac actin isoform ratios in cardiac muscle
Rapid Communications in Mass Spectrometry, 17(13), 1467–1471.
MeSH headings : Actins / analysis; Animals; Cattle; Mass Spectrometry; Muscle, Skeletal / chemistry; Myocardium / chemistry; Protein Isoforms / analysis; Rabbits
TL;DR:
A mass spectrometric technique to measure the ratio of skeletal to cardiac actin and a new dual electrospray ionization source was employed to determine accurate masses of the alpha-skeletal and alpha-cardiac actins.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2003 journal article
Advantages of Thermococcus kodakaraenis (KOD) DNA Polymerase for PCR-mass spectrometry based analyses
Journal of the American Society for Mass Spectrometry, 14(6), 601–604.
MeSH headings : DNA-Directed DNA Polymerase / chemistry; Fourier Analysis; Humans; Polymerase Chain Reaction / methods; Spectrometry, Mass, Electrospray Ionization; Taq Polymerase / chemistry; Thermococcus / enzymology; von Willebrand Factor / chemistry
TL;DR:
KOD DNA polymerase demonstrated a 2- to 3-fold increase in PCR product formation compared to Pfu or Taq, respectively, and generated blunt-ended PCR product which allows facile interpretation of the mass spectrum.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 12, 2021
2003 journal article
Implications of Hydrophobicity and Free Energy of Solvation for Characterization of Nucleic Acids by Electrospray Ionization Mass Spectrometry
Analytical Chemistry, 75(6), 1331–1339.
MeSH headings : Deoxyribonucleotides / analysis; Gene Frequency; Hydrophobic and Hydrophilic Interactions; Nucleic Acids / chemistry; Oligonucleotides / analysis; Polymorphism, Single Nucleotide; Solvents; Spectrometry, Mass, Electrospray Ionization / methods; Thermodynamics
TL;DR:
Investigation of the contributions of hydrophobicity and free energy of solvation to relative signal intensities in ESI-MS spectra of nucleic acids found a trend in signal intensity for deoxyribonucleotide triphosphates, oligonucleotides, and PCR amplicons was observed that was consistent with the model.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
7. Affordable and Clean Energy
(OpenAlex)
Sources: Crossref, ORCID
Added: February 12, 2021
2003 journal article
Predicting and Modeling Superintendent Turnover
Journal of School Leadership, 13(3), 328–346.
UN Sustainable Development Goal Categories
1. No Poverty
(OpenAlex)
Sources: Crossref, ORCID
Added: February 9, 2021
2003 journal article
Artificial cells: Unique insights into exocytosis using liposomes and lipid nanotubes
Proceedings of the National Academy of Sciences, 100(2), 400–404.
MeSH headings : Animals; Biological Transport; Exocytosis / physiology; Lipid Metabolism; Liposomes / metabolism; Membrane Fusion; Microscopy, Fluorescence; PC12 Cells; Rats
TL;DR:
The use of liposome–lipid nanotube networks are described to create an artificial cell model that undergoes the later stages of exocytosis and shows that membrane mechanics, without protein intervention, can drive expansion of the fusion pore to the final stage of excycletosis.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: February 5, 2021
2003 journal article
What’s Your Diagnosis?: Equine Cholelithiasis
Journal of the American Veterinary Medical Association, 222(3), 289–290.
MeSH headings : Animals; Anti-Bacterial Agents / therapeutic use; Cholelithiasis / diagnosis; Cholelithiasis / diagnostic imaging; Cholelithiasis / drug therapy; Cholelithiasis / veterinary; Cholestasis / diagnosis; Cholestasis / diagnostic imaging; Cholestasis / drug therapy; Cholestasis / veterinary; Horse Diseases / diagnosis; Horse Diseases / diagnostic imaging; Horse Diseases / drug therapy; Horses; Liver / diagnostic imaging; Liver / enzymology; Liver / pathology; Oxytetracycline / therapeutic use; Ultrasonography
Sources: Web Of Science, ORCID
Added: January 9, 2021
2003 journal article
Directed evolution: Creating new enzymes
The Biochemist, 25(4), 13–15.
Sources: NC State University Libraries, ORCID
Added: January 9, 2021
2003 journal article
The molecular structure and intermolecular interactions of 1,3:2,4-dibenzylidene-D-sorbitol
Molecular Physics, 101(19), 3017–3027.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Crossref, ORCID
Added: January 5, 2021
2003 journal article
Investigation of ligand loading and asymmetric amplification in CHAOx-catalyzed asymmetric diethylzinc additions
Tetrahedron: Asymmetry, 14(22), 3605–3611.
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Sources: Crossref, ORCID
Added: December 24, 2020
2003 chapter
Equine Recurrent Uveitis
In N. R. Robinson (Ed.), Current Therapy in Equine Medicine (5th edition, pp. 468–473). Philadelphia, PA: Saunders.
Ed(s): N. Robinson
Source: NC State University Libraries
Added: December 22, 2020
2003 journal article
Modifying the stereochemistry of an enzyme-catalyzed reaction by directed evolution
Proceedings of the National Academy of Sciences, 100(6), 3143–3148.
MeSH headings : Aldehyde-Lyases / chemistry; Aldehyde-Lyases / genetics; Aldehyde-Lyases / metabolism; Catalytic Domain; Directed Molecular Evolution; Escherichia coli / genetics; Fructosediphosphates / chemistry; Fructosediphosphates / metabolism; Hexosediphosphates / chemistry; Hexosediphosphates / metabolism; Kinetics; Models, Molecular; Mutation; Nuclear Magnetic Resonance, Biomolecular; Recombinant Proteins / chemistry; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Stereoisomerism; Substrate Specificity
TL;DR:
It is shown that directed evolution can be used to alter the stereochemical course of the reaction catalyzed by tagatose-1,6-bisphosphate aldolase, resulting in a 100-fold change in stereospecificity.
(via Semantic Scholar)
Sources: Crossref, ORCID
Added: December 22, 2020
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