North Carolina Agricultural Research Service

Works Published in 2011

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2011 journal article

Validation of the H2S method to detect bacteria of fecal origin by cultured and molecular methods

Applied Microbiology and Biotechnology, 92(6), 1287–1295.

By: L. McMahan*, A. Devine*, A. Grunden n & M. Sobsey*

author keywords: H2S test; TRFLP; Microbial water quality
MeSH headings : Amplified Fragment Length Polymorphism Analysis / methods; Bacteria / chemistry; Bacteria / genetics; Bacteria / isolation & purification; Bacteria / metabolism; Biochemistry / methods; Feces / microbiology; Hydrogen Sulfide / analysis; Hydrogen Sulfide / metabolism; Polymorphism, Restriction Fragment Length; Water Microbiology; Water Pollution / analysis
TL;DR: This research determined whether or not the H2S test did correctly identify sewage-contaminated waters by being the first to use culturing and molecular methods to identify the types and numbers of fecal indicator organisms, pathogens, and other microbes present in sewage samples with positive H1N1 test results. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Crossref, ORCID
Added: September 8, 2020

2011 journal article

Improving the Catalytic Activity of Hyperthermophilic Pyrococcus horikoshii Prolidase for Detoxification of Organophosphorus Nerve Agents over a Broad Range of Temperatures

ARCHAEA-AN INTERNATIONAL MICROBIOLOGICAL JOURNAL, 2011.

By: C. Theriot*, R. Semcer n, S. Shah & A. Grunden n

MeSH headings : Biotransformation; Chemical Warfare Agents / metabolism; Dipeptidases / genetics; Dipeptidases / metabolism; Enzyme Stability; Hydrogen-Ion Concentration; Mutant Proteins / genetics; Mutant Proteins / metabolism; Mutation; Organophosphates / metabolism; Pyrococcus horikoshii / chemistry; Pyrococcus horikoshii / enzymology; Pyrococcus horikoshii / metabolism; Sarin / metabolism; Soman / metabolism; Temperature
TL;DR: Four Ph1prol mutants were isolated which had greater thermostability and improved activity over a broader range of temperatures against Xaa-Pro dipeptides and OP nerve agents compared to wild type Pyrococcus prolidases. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2011 journal article

Determination of aflatoxin risk components for in-shell Brazil nuts

FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 28(9), 1242–1260.

By: E. Vargas*, E. Santos*, T. Whitaker n & A. Slate n

author keywords: survey; exposure; risk assessment; mycotoxins; aflatoxins
MeSH headings : Aflatoxins / chemistry; Bertholletia / chemistry; Brazil; Food Analysis; Food Contamination / analysis; Food Handling; Nuts / chemistry
TL;DR: Rotten nuts were found to be a high-risk fraction for aflatoxin in in-shell Brazil nut lots, and the highest correlations were found between the a Flatoxins concentration inin in- shell Brazil nuts samples and the a flatoxin concentration in all defective fractions. (via Semantic Scholar)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Effect of Simulated Aminocyclopyrachlor Drift on Flue-Cured Tobacco

WEED TECHNOLOGY, 25(4), 609–615.

author keywords: Off-target movement; synthetic auxin
TL;DR: Flue-cured tobacco is sensitive to foliar and soil residues of off-target synthetic auxin drift, and herbicide rates and application timings caused significant visual tobacco injury, ranging from slight to severe with increasing herbicide drift rates. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
3. Good Health and Well-being (OpenAlex)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2011 journal article

Pectobacterium carotovorum Elicits Plant Cell Death with DspE/F but the P. carotovorum DspE Does Not Suppress Callose or Induce Expression of Plant Genes Early in Plant-Microbe Interactions

MOLECULAR PLANT-MICROBE INTERACTIONS, 24(7), 773–786.

By: H. Kim, P. Thammarat, S. Lommel*, C. Hogan & A. Charkowski

MeSH headings : Amino Acid Sequence; Bacterial Proteins / metabolism; Bacterial Secretion Systems / genetics; Bacterial Secretion Systems / physiology; Cell Death; Gene Expression Profiling; Gene Expression Regulation; Gene Expression Regulation, Plant; Glucans / metabolism; Host-Pathogen Interactions; Mitogen-Activated Protein Kinases / metabolism; Mutation; Pectobacterium carotovorum / genetics; Pectobacterium carotovorum / metabolism; Pectobacterium carotovorum / pathogenicity; Phenotype; Plant Diseases / genetics; Plant Diseases / microbiology; Plant Immunity; Plant Leaves / microbiology; Pseudomonas syringae / genetics; Pseudomonas syringae / pathogenicity; Sequence Alignment; Tobacco / cytology; Tobacco / genetics; Tobacco / metabolism; Tobacco / microbiology; Virulence Factors
TL;DR: A model where Pectobacterium carotovorum uses the T3SS to induce plant cell death in order to promote leaf maceration rather than to suppress plant immunity is supported. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: August 6, 2018

2011 review

Hydrolysis of organophosphorus compounds by microbial enzymes

[Review of ]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 89(1), 35–43.

By: C. Theriot n & A. Grunden n

author keywords: Organophosphorus compound; OP nerve agent; Pesticide; OPAA; OPH; Phosphotriesterase; Prolidase
MeSH headings : Aryldialkylphosphatase / chemistry; Aryldialkylphosphatase / genetics; Aryldialkylphosphatase / metabolism; Bacteria / chemistry; Bacteria / enzymology; Bacterial Proteins / chemistry; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Biocatalysis; Hydrolysis; Kinetics; Organophosphorus Compounds / chemistry; Substrate Specificity
TL;DR: An update of what is experimentally known about OPH and OPAA to include their structures, substrate specificity, and catalytic properties is provided. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
Sources: Web Of Science, ORCID
Added: August 6, 2018

2011 journal article

The Red clover necrotic mosaic virus Capsid as a Multifunctional Cell Targeting Plant Viral Nanoparticle

BIOCONJUGATE CHEMISTRY, 22(1), 67–73.

By: D. Lockney n, R. Guenther n, L. Loo n, W. Overton n, R. Antonelli n, J. Clark n, M. Hu n, C. Luft n, S. Lommel n, S. Franzen n

MeSH headings : Amino Acid Sequence; Biological Availability; Biological Transport; Capsid / chemistry; Capsid / metabolism; Doxorubicin / chemistry; Doxorubicin / pharmacokinetics; Fluorescent Dyes / chemistry; HeLa Cells; Humans; Models, Molecular; Nanoparticles / chemistry; Peptides / chemistry; Peptides / metabolism; Plants / virology; Protein Conformation; Surface Properties; Tombusviridae
TL;DR: It is shown that targeting peptides (less than 16 amino acids) can be conjugated to the capsid using the heterobifunctional chemical linker sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC). (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: August 6, 2018

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