2023 journal article
Blood Supplementation Enhances Bartonella henselae Growth and Molecular Detection of Bacterial DNA in Liquid Culture
Microbiology Spectrum.
Ed(s): H. Mostafa
MeSH headings : Humans; Animals; Horses / genetics; Dogs; Sheep; Bartonella henselae / genetics; DNA, Bacterial / genetics; DNA, Bacterial / analysis; Bartonella Infections / diagnosis; Bartonella Infections / microbiology; Bartonella Infections / veterinary; Bartonella / genetics; Dietary Supplements; Mammals
TL;DR:
The addition of sheep blood to liquid culture media increased the Bartonella henselae DNA concentration compared to controls, additionally improving PCR direct detection sensitivity.
(via Semantic Scholar)
doi.org (publisher)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: May 26, 2023
This study aims to improve diagnostic detection of Bartonella henselae . Patient samples are combined with enriched bacterial cultures aimed at growing Bartonella henselae for the best possible chance at detection. However, current Bartonella growth methods could be improved. The DNA extraction method used by most laboratories should also be optimized. Sheep blood was added to increase the growth of Bartonella henselae and multiple DNA extraction methods were to be compared to each other.