2023 article

Exceptionally high genetic variance of the doubled haploid (DH) population of poplar

Liu, C., Wang, S., Liu, Y., Wang, M., Fan, E., Liu, C., … Qu, G. (2023, May 4). JOURNAL OF FORESTRY RESEARCH.

By: C. Liu*, S. Wang*, Y. Liu*, M. Wang*, E. Fan*, C. Liu*, S. Zhang*, C. Yang* ...

author keywords: Populus; Anther culture; Doubled haploid; Genetic variance; Flow cytometry
TL;DR: This study provides an effective protocol for poplar haploid induction based on an anther culture method and shows that some excellent homozygous lines have the potential to be model plants in genetic and breeding studies. (via Semantic Scholar)
UN Sustainable Development Goal Categories
13. Climate Action (Web of Science)
15. Life on Land (Web of Science)
Source: Web Of Science
Added: May 30, 2023

AbstractDoubled haploid (DH) plants have been widely used for breeding and biological research in crops. Populus spp. have been used as model woody plant species for biological research. However, the induction of DH poplar plants is onerous, and limited biological or breeding work has been carried out on DH individuals or populations. In this study, we provide an effective protocol for poplar haploid induction based on an anther culture method. A total of 96 whole DH plant lines were obtained using an F1 hybrid of Populus simonii × P. nigra as a donor tree. The phenotypes of the DH population showed exceptionally high variance when compared to those of half-sib progeny of the donor tree. Each DH line displayed distinct features compared to those of the other DH lines or the donor tree. Additionally, some excellent homozygous lines have the potential to be model plants in genetic and breeding studies.