Characterization of integration sites and transfer DNA structures in Agrobacterium-mediated transgenic events of maize inbred B104
Neelakandan, A. K., Kabahuma, M., Yang, Q., Lopez, M., Wisser, R. J., Balint-Kurti, P., & Lauter, N. (2023, July 31). G3-GENES GENOMES GENETICS.
author keywords: maize; T-DNA; transformation; nonhomologous end-joining; integration; Agrobacterium; genetic engineering; disease resistance; Plant Genetics and Genomics
TL;DR:
A quantitative assessment of Agrobacterium-mediated T-DNA integration in maize is provided with respect to insertion site features, the genomic distribution of T- DNA incorporation, and mechanisms of integration, and the utility of the FPNI-PCR technique is demonstrated.
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