2023 article
Improvements in the detection power of algorithms for analyzing next-generation sequencing based bulked segregant analysis data via estimating thresholds at the genomic region level
Zhang, J., & Panthee, D. R. (2023, March 13).
TL;DR:
This work estimated the confidence intervals/thresholds of the popular algorithms at the genomic region level and increased the detection power of these algorithms by at least 5 folds, which should drastically reduce the sequencing cost of BSA-Seq studies.
(via Semantic Scholar)
open access via doi.org (repository)
Source: ORCID
Added: January 28, 2024
AbstractNext-generation sequencing based bulked segregant analysis (BSA-Seq) has been widely used in identifying genomic regions associated with a trait of interest. However, the most popular algorithms for BSA-Seq data analysis have relatively low detection power, and high sequencing depths are required for the detection of genomic regions linked to the trait. Here we estimated the confidence intervals/thresholds of the popular algorithms at the genomic region level and increased the detection power of these algorithms by at least 5 folds, which should drastically reduce the sequencing cost of BSA-Seq studies.