2023 article

A normalization method that controls for total RNA abundance affects the identification of differentially expressed genes, revealing bias toward morning-expressed responses

Laosuntisuk, K., Vennapusa, A., Somayanda, I. M., Leman, A. R., Jagadish, S. V. K., & Doherty, C. J. (2023, October 29).

By: K. Laosuntisuk  n, A. Vennapusa *, I. Somayanda *, A. Leman, S. Jagadish * & C. Doherty n 

TL;DR: It is demonstrated that adding external RNA spike-ins as a normalization control produces differences in RNA-Seq analysis compared to traditional normalization methods, even between two times of day in untreated plants. (via Semantic Scholar)
Source: ORCID
Added: February 1, 2024

Abstract