2024 article

Offline Two-Dimensional Liquid Chromatography-Mass Spectrometry for Deep Annotation of the Fecal Metabolome Following Fecal Microbiota Transplantation

Anderson, B. G., Raskind, A., Hissong, R., Dougherty, M. K., McGill, S. K., Gulati, A. S., … Evans, C. R. (2024, May 16). JOURNAL OF PROTEOME RESEARCH.

By: B. Anderson *, A. Raskind *, R. Hissong*, M. Dougherty *, S. McGill *, A. Gulati *, C. Theriot n, R. Kennedy *, C. Evans *

author keywords: Clostridioides difficile; C. diff; HILIC; RPLC; LC x LC; untargeted metabolomics; compound identification; bile acids; LC-MS; MS/MS
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: May 28, 2024

Biological interpretation of untargeted LC-MS-based metabolomics data depends on accurate compound identification, but current techniques fall short of identifying most features that can be detected. The human fecal metabolome is complex, variable, incompletely annotated, and serves as an ideal matrix to evaluate novel compound identification methods. We devised an experimental strategy for compound annotation using multidimensional chromatography and semiautomated feature alignment and applied these methods to study the fecal metabolome in the context of fecal microbiota transplantation (FMT) for recurrent