2024 journal article
Taxanorm: a novel taxa-specific normalization approach for microbiome data
BMC BIOINFORMATICS, 25(1).
author keywords: Sequencing depth; Normalization; Microbiome; High-throughput sequencing
open access via doi.org (publisher)
Source: Web Of Science
Added: September 23, 2024
In high-throughput sequencing studies, sequencing depth, which quantifies the total number of reads, varies across samples. Unequal sequencing depth can obscure true biological signals of interest and prevent direct comparisons between samples. To remove variability due to differential sequencing depth, taxa counts are usually normalized before downstream analysis. However, most existing normalization methods scale counts using size factors that are sample specific but not taxa specific, which can result in over- or under-correction for some taxa.